Induction of micronuclei by cadmium chloride in AO rats depends on age and sex
2014
Authors
Popović-Bubujuk, SlavicaMuster, Adrian
Đelić, Ninoslav
Kataranovski, Dragan
Anđelković, Marko
Article (Published version)
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Cadmium (Cd) is one of the most toxic industrial and metal in the environment which may cause severe genotoxic effects. The aim of the work was an evaluation of genotoxic effects of CdCl2 in genetically pure Albino Oxford (AO) rats, depending on sex, age and dosage. Experimental animals were treated intraperitoneally with three different concentrations of CdCl2: 0.5, 1, and 2 mg/kg of CdCl2, while the control animals received equal volume of sterile phosphate buffered saline. The individuals of both sexes were treated at the age of 3, 6 and 12 month. Frequency of micronuclei formation was evaluated in polychromatic erythrocytes (PCEs), 24h hours after the treatment. The results showed that CdCl2 caused a concentration-dependent increase of micronucleus frequency. The most significant differences were found between ages of 3/12 and 6/12 months at 0.5 and 1.0 mg CdCl2 concentrations. Namely, 3 month old males had higher frequency of MNi in comparison to 12 month old males, whereas in fem...ales it was the opposite. Likewise, 6 months old males exbibited greater sensistivity to CdCl2 in comparison to 12 month old rats, and in the females it was the opposite. Sex differences were further confirmed as slightly stronger genotoxic effects in 12 months old females treated with 0.5 and 1 mg/kg of CdCl2. Therefore, the genotoxic effects of cadmium in AO rats depend on concentration, age and sex.
Keywords:
cadmium / micronuclei / Albino Oxford ratsSource:
Genetika, 2014, 46, 3, 1003-1012Publisher:
- Društvo genetičara Srbije, Beograd
Funding / projects:
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
- Dynamics of gene pool, genetic and phenotypic variability of populations, determined by the environmental changes (RS-MESTD-Basic Research (BR or ON)-173012)
DOI: 10.2298/GENSR1403003P
ISSN: 0534-0012
WoS: 000348608200031
Scopus: 2-s2.0-84922155724
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Popović-Bubujuk, Slavica AU - Muster, Adrian AU - Đelić, Ninoslav AU - Kataranovski, Dragan AU - Anđelković, Marko PY - 2014 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1097 AB - Cadmium (Cd) is one of the most toxic industrial and metal in the environment which may cause severe genotoxic effects. The aim of the work was an evaluation of genotoxic effects of CdCl2 in genetically pure Albino Oxford (AO) rats, depending on sex, age and dosage. Experimental animals were treated intraperitoneally with three different concentrations of CdCl2: 0.5, 1, and 2 mg/kg of CdCl2, while the control animals received equal volume of sterile phosphate buffered saline. The individuals of both sexes were treated at the age of 3, 6 and 12 month. Frequency of micronuclei formation was evaluated in polychromatic erythrocytes (PCEs), 24h hours after the treatment. The results showed that CdCl2 caused a concentration-dependent increase of micronucleus frequency. The most significant differences were found between ages of 3/12 and 6/12 months at 0.5 and 1.0 mg CdCl2 concentrations. Namely, 3 month old males had higher frequency of MNi in comparison to 12 month old males, whereas in females it was the opposite. Likewise, 6 months old males exbibited greater sensistivity to CdCl2 in comparison to 12 month old rats, and in the females it was the opposite. Sex differences were further confirmed as slightly stronger genotoxic effects in 12 months old females treated with 0.5 and 1 mg/kg of CdCl2. Therefore, the genotoxic effects of cadmium in AO rats depend on concentration, age and sex. PB - Društvo genetičara Srbije, Beograd T2 - Genetika T1 - Induction of micronuclei by cadmium chloride in AO rats depends on age and sex VL - 46 IS - 3 SP - 1003 EP - 1012 DO - 10.2298/GENSR1403003P ER -
@article{ author = "Popović-Bubujuk, Slavica and Muster, Adrian and Đelić, Ninoslav and Kataranovski, Dragan and Anđelković, Marko", year = "2014", abstract = "Cadmium (Cd) is one of the most toxic industrial and metal in the environment which may cause severe genotoxic effects. The aim of the work was an evaluation of genotoxic effects of CdCl2 in genetically pure Albino Oxford (AO) rats, depending on sex, age and dosage. Experimental animals were treated intraperitoneally with three different concentrations of CdCl2: 0.5, 1, and 2 mg/kg of CdCl2, while the control animals received equal volume of sterile phosphate buffered saline. The individuals of both sexes were treated at the age of 3, 6 and 12 month. Frequency of micronuclei formation was evaluated in polychromatic erythrocytes (PCEs), 24h hours after the treatment. The results showed that CdCl2 caused a concentration-dependent increase of micronucleus frequency. The most significant differences were found between ages of 3/12 and 6/12 months at 0.5 and 1.0 mg CdCl2 concentrations. Namely, 3 month old males had higher frequency of MNi in comparison to 12 month old males, whereas in females it was the opposite. Likewise, 6 months old males exbibited greater sensistivity to CdCl2 in comparison to 12 month old rats, and in the females it was the opposite. Sex differences were further confirmed as slightly stronger genotoxic effects in 12 months old females treated with 0.5 and 1 mg/kg of CdCl2. Therefore, the genotoxic effects of cadmium in AO rats depend on concentration, age and sex.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika", title = "Induction of micronuclei by cadmium chloride in AO rats depends on age and sex", volume = "46", number = "3", pages = "1003-1012", doi = "10.2298/GENSR1403003P" }
Popović-Bubujuk, S., Muster, A., Đelić, N., Kataranovski, D.,& Anđelković, M.. (2014). Induction of micronuclei by cadmium chloride in AO rats depends on age and sex. in Genetika Društvo genetičara Srbije, Beograd., 46(3), 1003-1012. https://doi.org/10.2298/GENSR1403003P
Popović-Bubujuk S, Muster A, Đelić N, Kataranovski D, Anđelković M. Induction of micronuclei by cadmium chloride in AO rats depends on age and sex. in Genetika. 2014;46(3):1003-1012. doi:10.2298/GENSR1403003P .
Popović-Bubujuk, Slavica, Muster, Adrian, Đelić, Ninoslav, Kataranovski, Dragan, Anđelković, Marko, "Induction of micronuclei by cadmium chloride in AO rats depends on age and sex" in Genetika, 46, no. 3 (2014):1003-1012, https://doi.org/10.2298/GENSR1403003P . .