Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats
2015
Autori
Roksandić, DragutinRadovanović, Anita
Danilović-Luković, Jelena
Marković, Danica
Kovačević-Filipović, Milica
Colić, Miodrag
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The purpose of this work was to investigate the influence of hypothyroidism on spleen tissue morphology and immune cell density in fourteen-day-old juvenile rats. Hypothyroidism in pups (n=10) was induced by administration of propylthiouracil (PTU) in drinking water (1.5 mg/L) to their mothers during pregnancy and period of lactation. Fourteen-day-old pups were sacrificed and the thyroid-stimulating hormone (TSH) serum concentration and thyroid activation index (Ia) were determined. Increased serum level of TSH and increased Ia showed that pups from PTU treated mothers were hypothyroid. White and red spleen pulp, marginal zone and connective tissue volume density has been assessed by using the stereological method. Using immunohistochemistry, the present CD3+ T lymphocytes, CD45RA+ B lymphocytes and CD68+ macrophages were quantified. A significant reduction of volume density of the periarteriolar lymphocyte sheath (Vv(PALS)) and lymphatic follicles (Vvf) due to depletion of T and B lym...phocytes respectively, was observed in the spleens of hypothyroid pups compared to controls. The volume density of the red pulp (Vvrp), marginal zone (Vvmz) and connective tissue (Vvct) was increased, as well as the number of CD68+ macrophages in the spleens of hypothyroid pups compared to controls. These results indicate that thyroid hormones might be important for normal development of both, specific and innate immune cells in the spleen during prenatal and early postnatal period.
Ključne reči:
spleen / hypothyroidism / stereology / immunohistochemistry / ratIzvor:
Acta Veterinaria-Beograd, 2015, 65, 2, 246-259Izdavač:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Finansiranje / projekti:
- Antioksidativna zaštita i potencijali za diferencijaciju i regeneraciju mezenhimalnih matičnih ćelija iz različitih tkiva tokom procesa starenja (RS-MESTD-Basic Research (BR or ON)-175061)
DOI: 10.1515/acve-2015-0021
ISSN: 0567-8315
WoS: 000356832100009
Scopus: 2-s2.0-84933511782
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Roksandić, Dragutin AU - Radovanović, Anita AU - Danilović-Luković, Jelena AU - Marković, Danica AU - Kovačević-Filipović, Milica AU - Colić, Miodrag PY - 2015 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1231 AB - The purpose of this work was to investigate the influence of hypothyroidism on spleen tissue morphology and immune cell density in fourteen-day-old juvenile rats. Hypothyroidism in pups (n=10) was induced by administration of propylthiouracil (PTU) in drinking water (1.5 mg/L) to their mothers during pregnancy and period of lactation. Fourteen-day-old pups were sacrificed and the thyroid-stimulating hormone (TSH) serum concentration and thyroid activation index (Ia) were determined. Increased serum level of TSH and increased Ia showed that pups from PTU treated mothers were hypothyroid. White and red spleen pulp, marginal zone and connective tissue volume density has been assessed by using the stereological method. Using immunohistochemistry, the present CD3+ T lymphocytes, CD45RA+ B lymphocytes and CD68+ macrophages were quantified. A significant reduction of volume density of the periarteriolar lymphocyte sheath (Vv(PALS)) and lymphatic follicles (Vvf) due to depletion of T and B lymphocytes respectively, was observed in the spleens of hypothyroid pups compared to controls. The volume density of the red pulp (Vvrp), marginal zone (Vvmz) and connective tissue (Vvct) was increased, as well as the number of CD68+ macrophages in the spleens of hypothyroid pups compared to controls. These results indicate that thyroid hormones might be important for normal development of both, specific and innate immune cells in the spleen during prenatal and early postnatal period. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats VL - 65 IS - 2 SP - 246 EP - 259 DO - 10.1515/acve-2015-0021 ER -
@article{ author = "Roksandić, Dragutin and Radovanović, Anita and Danilović-Luković, Jelena and Marković, Danica and Kovačević-Filipović, Milica and Colić, Miodrag", year = "2015", abstract = "The purpose of this work was to investigate the influence of hypothyroidism on spleen tissue morphology and immune cell density in fourteen-day-old juvenile rats. Hypothyroidism in pups (n=10) was induced by administration of propylthiouracil (PTU) in drinking water (1.5 mg/L) to their mothers during pregnancy and period of lactation. Fourteen-day-old pups were sacrificed and the thyroid-stimulating hormone (TSH) serum concentration and thyroid activation index (Ia) were determined. Increased serum level of TSH and increased Ia showed that pups from PTU treated mothers were hypothyroid. White and red spleen pulp, marginal zone and connective tissue volume density has been assessed by using the stereological method. Using immunohistochemistry, the present CD3+ T lymphocytes, CD45RA+ B lymphocytes and CD68+ macrophages were quantified. A significant reduction of volume density of the periarteriolar lymphocyte sheath (Vv(PALS)) and lymphatic follicles (Vvf) due to depletion of T and B lymphocytes respectively, was observed in the spleens of hypothyroid pups compared to controls. The volume density of the red pulp (Vvrp), marginal zone (Vvmz) and connective tissue (Vvct) was increased, as well as the number of CD68+ macrophages in the spleens of hypothyroid pups compared to controls. These results indicate that thyroid hormones might be important for normal development of both, specific and innate immune cells in the spleen during prenatal and early postnatal period.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats", volume = "65", number = "2", pages = "246-259", doi = "10.1515/acve-2015-0021" }
Roksandić, D., Radovanović, A., Danilović-Luković, J., Marković, D., Kovačević-Filipović, M.,& Colić, M.. (2015). Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(2), 246-259. https://doi.org/10.1515/acve-2015-0021
Roksandić D, Radovanović A, Danilović-Luković J, Marković D, Kovačević-Filipović M, Colić M. Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats. in Acta Veterinaria-Beograd. 2015;65(2):246-259. doi:10.1515/acve-2015-0021 .
Roksandić, Dragutin, Radovanović, Anita, Danilović-Luković, Jelena, Marković, Danica, Kovačević-Filipović, Milica, Colić, Miodrag, "Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats" in Acta Veterinaria-Beograd, 65, no. 2 (2015):246-259, https://doi.org/10.1515/acve-2015-0021 . .