KIT receptor expression in canine cutaneous mast cell tumors (CMCTS) without c-kit mutation
Ekspresija KIT receptora na kutanim mastocitomima pasa bez c-kit mutacija
2016
Authors
Vučićević, IvanaMarinković, Darko
Kukolj, Vladimir
Vučićević, Miloš
Mirilović, Milorad
Nešić, Slađan
Aleksić-Kovačević, Sanja
Article (Published version)
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Histopathological examination, grading, immunohistochemical staining and molecular genetic examinations are the proposed criteria that should be used for cutaneous mast cell tumors (CMCTs) classification. The presence of aberrant CD117 expression and mutations of the c-kit proto-oncogene could be an indicative parameter for final histological grading. Determination of the connection between the localization of KIT receptor expression and the histological grade of CMCTs without c-kit proto-oncogene mutations was the main goal of this study. The study included twenty four CMCTs and six control skin samples from 30 dogs of different ages, breed and sex. Formalinfixed and paraffin-embedded tissue samples were stained with hematoxylin-eosin and toluidine blue and immunohistochemically tested for CD117 expression. DNA was extracted from the same paraffin blocks and subsequent polymerase chain reaction amplification was performed using PE1 and PE2 primers. Degree of malignancy was determined ...based on the presence of mitotic figures, multinucleated cells, bizarre nuclei and karyomegaly in 10 high power fields. Based on histological features, fourteen of 24 CMCTs were of a high histological grade, while ten were classified as a lowgrade malignancy. CD117 cytoplasmic expression was observed in nine of fourteen high-grade malignancy CMCTs, which confirms the link between the aberrant CD117 expression and increased cell proliferation.
Histopatološko ispitivanje, gradacija, imunohistohemijsko bojenje i molekularno genetska ispitivanja su predloženi kriterijumi za klasifikaciju kutanih mastocitoma pasa. Prisustvo aberantne ekspresije CD117 i mutacija c-kit protoonkogena moglo bi da bude indikativni parameter za finalnu histopatološku klasifikaciju mastocitoma. Glavni cilj ispitivanja je određivanje povezanosti mesta ekspresije KIT receptora i histološkog gradusa mastocitoma bez c-kit mutacija. Ispitivanjima su obuhvaćena 24 mastocitoma i 6 kontrolnih isečaka kože uzorkovanih od ukupno 30 odraslih pasa različitog uzrasta, rase i pola. Uzorci fiksirani u formalinu i ukalupljeni u parafinske kalupe su bojeni hematoksilin-eozinom, toluidin plavo metodom i imunohistohemijski primenom CD117 antitela. DNK je izolovana iz istih parafinskih kalupa, a PCR amplifikacija je izvršena primenom specifičnih prajmera PE1 i PE2. Stepen malignosti je utvrđen na osnovu prisustva mitotskih figura, multinuklearnih ćelija, ćelija sa bizarni...m jedrom i kariomegalije na 10 polja velikog uveličanja. Na osnovu histopatoloških osobina, 14 od 24 mastocitoma su svrstani u visoki stepen maligniteta, dok je 10 mastocitoma niskog stepena maligniteta. Citoplazmatska ekspresija CD117 je uočena kod 9 od 14 mastocitoma visokog stepena maligniteta, što potvrđuje vezu između aberantne ekspresije CD117 i povećane ćelijske proliferacije.
Keywords:
dogs / mast cell tumor / morphology / classification / CD117Source:
Acta Veterinaria-Beograd, 2016, 66, 2, 222-233Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.1515/acve-2016-0019
ISSN: 0567-8315
WoS: 000384770600007
Scopus: 2-s2.0-84978828723
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Vučićević, Ivana AU - Marinković, Darko AU - Kukolj, Vladimir AU - Vučićević, Miloš AU - Mirilović, Milorad AU - Nešić, Slađan AU - Aleksić-Kovačević, Sanja PY - 2016 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1361 AB - Histopathological examination, grading, immunohistochemical staining and molecular genetic examinations are the proposed criteria that should be used for cutaneous mast cell tumors (CMCTs) classification. The presence of aberrant CD117 expression and mutations of the c-kit proto-oncogene could be an indicative parameter for final histological grading. Determination of the connection between the localization of KIT receptor expression and the histological grade of CMCTs without c-kit proto-oncogene mutations was the main goal of this study. The study included twenty four CMCTs and six control skin samples from 30 dogs of different ages, breed and sex. Formalinfixed and paraffin-embedded tissue samples were stained with hematoxylin-eosin and toluidine blue and immunohistochemically tested for CD117 expression. DNA was extracted from the same paraffin blocks and subsequent polymerase chain reaction amplification was performed using PE1 and PE2 primers. Degree of malignancy was determined based on the presence of mitotic figures, multinucleated cells, bizarre nuclei and karyomegaly in 10 high power fields. Based on histological features, fourteen of 24 CMCTs were of a high histological grade, while ten were classified as a lowgrade malignancy. CD117 cytoplasmic expression was observed in nine of fourteen high-grade malignancy CMCTs, which confirms the link between the aberrant CD117 expression and increased cell proliferation. AB - Histopatološko ispitivanje, gradacija, imunohistohemijsko bojenje i molekularno genetska ispitivanja su predloženi kriterijumi za klasifikaciju kutanih mastocitoma pasa. Prisustvo aberantne ekspresije CD117 i mutacija c-kit protoonkogena moglo bi da bude indikativni parameter za finalnu histopatološku klasifikaciju mastocitoma. Glavni cilj ispitivanja je određivanje povezanosti mesta ekspresije KIT receptora i histološkog gradusa mastocitoma bez c-kit mutacija. Ispitivanjima su obuhvaćena 24 mastocitoma i 6 kontrolnih isečaka kože uzorkovanih od ukupno 30 odraslih pasa različitog uzrasta, rase i pola. Uzorci fiksirani u formalinu i ukalupljeni u parafinske kalupe su bojeni hematoksilin-eozinom, toluidin plavo metodom i imunohistohemijski primenom CD117 antitela. DNK je izolovana iz istih parafinskih kalupa, a PCR amplifikacija je izvršena primenom specifičnih prajmera PE1 i PE2. Stepen malignosti je utvrđen na osnovu prisustva mitotskih figura, multinuklearnih ćelija, ćelija sa bizarnim jedrom i kariomegalije na 10 polja velikog uveličanja. Na osnovu histopatoloških osobina, 14 od 24 mastocitoma su svrstani u visoki stepen maligniteta, dok je 10 mastocitoma niskog stepena maligniteta. Citoplazmatska ekspresija CD117 je uočena kod 9 od 14 mastocitoma visokog stepena maligniteta, što potvrđuje vezu između aberantne ekspresije CD117 i povećane ćelijske proliferacije. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - KIT receptor expression in canine cutaneous mast cell tumors (CMCTS) without c-kit mutation T1 - Ekspresija KIT receptora na kutanim mastocitomima pasa bez c-kit mutacija VL - 66 IS - 2 SP - 222 EP - 233 DO - 10.1515/acve-2016-0019 ER -
@article{ author = "Vučićević, Ivana and Marinković, Darko and Kukolj, Vladimir and Vučićević, Miloš and Mirilović, Milorad and Nešić, Slađan and Aleksić-Kovačević, Sanja", year = "2016", abstract = "Histopathological examination, grading, immunohistochemical staining and molecular genetic examinations are the proposed criteria that should be used for cutaneous mast cell tumors (CMCTs) classification. The presence of aberrant CD117 expression and mutations of the c-kit proto-oncogene could be an indicative parameter for final histological grading. Determination of the connection between the localization of KIT receptor expression and the histological grade of CMCTs without c-kit proto-oncogene mutations was the main goal of this study. The study included twenty four CMCTs and six control skin samples from 30 dogs of different ages, breed and sex. Formalinfixed and paraffin-embedded tissue samples were stained with hematoxylin-eosin and toluidine blue and immunohistochemically tested for CD117 expression. DNA was extracted from the same paraffin blocks and subsequent polymerase chain reaction amplification was performed using PE1 and PE2 primers. Degree of malignancy was determined based on the presence of mitotic figures, multinucleated cells, bizarre nuclei and karyomegaly in 10 high power fields. Based on histological features, fourteen of 24 CMCTs were of a high histological grade, while ten were classified as a lowgrade malignancy. CD117 cytoplasmic expression was observed in nine of fourteen high-grade malignancy CMCTs, which confirms the link between the aberrant CD117 expression and increased cell proliferation., Histopatološko ispitivanje, gradacija, imunohistohemijsko bojenje i molekularno genetska ispitivanja su predloženi kriterijumi za klasifikaciju kutanih mastocitoma pasa. Prisustvo aberantne ekspresije CD117 i mutacija c-kit protoonkogena moglo bi da bude indikativni parameter za finalnu histopatološku klasifikaciju mastocitoma. Glavni cilj ispitivanja je određivanje povezanosti mesta ekspresije KIT receptora i histološkog gradusa mastocitoma bez c-kit mutacija. Ispitivanjima su obuhvaćena 24 mastocitoma i 6 kontrolnih isečaka kože uzorkovanih od ukupno 30 odraslih pasa različitog uzrasta, rase i pola. Uzorci fiksirani u formalinu i ukalupljeni u parafinske kalupe su bojeni hematoksilin-eozinom, toluidin plavo metodom i imunohistohemijski primenom CD117 antitela. DNK je izolovana iz istih parafinskih kalupa, a PCR amplifikacija je izvršena primenom specifičnih prajmera PE1 i PE2. Stepen malignosti je utvrđen na osnovu prisustva mitotskih figura, multinuklearnih ćelija, ćelija sa bizarnim jedrom i kariomegalije na 10 polja velikog uveličanja. Na osnovu histopatoloških osobina, 14 od 24 mastocitoma su svrstani u visoki stepen maligniteta, dok je 10 mastocitoma niskog stepena maligniteta. Citoplazmatska ekspresija CD117 je uočena kod 9 od 14 mastocitoma visokog stepena maligniteta, što potvrđuje vezu između aberantne ekspresije CD117 i povećane ćelijske proliferacije.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "KIT receptor expression in canine cutaneous mast cell tumors (CMCTS) without c-kit mutation, Ekspresija KIT receptora na kutanim mastocitomima pasa bez c-kit mutacija", volume = "66", number = "2", pages = "222-233", doi = "10.1515/acve-2016-0019" }
Vučićević, I., Marinković, D., Kukolj, V., Vučićević, M., Mirilović, M., Nešić, S.,& Aleksić-Kovačević, S.. (2016). KIT receptor expression in canine cutaneous mast cell tumors (CMCTS) without c-kit mutation. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(2), 222-233. https://doi.org/10.1515/acve-2016-0019
Vučićević I, Marinković D, Kukolj V, Vučićević M, Mirilović M, Nešić S, Aleksić-Kovačević S. KIT receptor expression in canine cutaneous mast cell tumors (CMCTS) without c-kit mutation. in Acta Veterinaria-Beograd. 2016;66(2):222-233. doi:10.1515/acve-2016-0019 .
Vučićević, Ivana, Marinković, Darko, Kukolj, Vladimir, Vučićević, Miloš, Mirilović, Milorad, Nešić, Slađan, Aleksić-Kovačević, Sanja, "KIT receptor expression in canine cutaneous mast cell tumors (CMCTS) without c-kit mutation" in Acta Veterinaria-Beograd, 66, no. 2 (2016):222-233, https://doi.org/10.1515/acve-2016-0019 . .