Functionalization of polycaprolactone/hydroxyapatite scaffolds with Usnea lethariiformis extract by using supercritical CO2
Само за регистроване кориснике
2016
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Investigation of an integrated supercritical fluid extraction and supercritical solvent impregnation process for fabrication of microporous polycaprolactone-hydroxyapatite (PCL-HA) scaffolds with antibacterial activity is presented. The HA content and particle size as well as the operating conditions of the integrated process is optimized regarding the amount of impregnated antibacterial agent (Usnea lethariiformis extract) in the PCL-HA matrix, scaffold morphology and antibacterial activity against methicillin resistant Staphylococcus aureus (MRSA) strains. High pressure differential scanning calorimetry (HP-DSC) assay reveals that an increasing amount of HA results in decreasing melting temperature as well as crystallinity at an operating pressure of 17 MPa. The PCL-HA composites with micrometric sizes of the HA particles are convenient for being processed by the integrated process due to the simple preparation, a good interaction between the PCL matrix and filler and the advantageou...s impact on sorption. The scaffold obtained from PCL-HA with 20% of the HA shows the highest impregnation yield at 17 MPa and 35 degrees C (5.9%) and subsequently also the best bactericidal effect on the tested MRSA strains at an initial bacterial inoculum of 2 x 10(-4) CFU/mL
Извор:
Materials Science & Engineering C-Materials for Biological Applications, 2016, 58, 204-212Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- National Research Council (CONICET, Argentina)Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) [PIP1851]
- University of Mar del Plata (Argentina) [15G/388]
- Technical University of Hamburg, Hamburg (Germany)
- Функционални физиолошки активни биљни материјали са додатом вредношћу за примену у фармацеутској и прехрамбеној индустрији (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45017)
DOI: 10.1016/j.msec.2015.08.024
ISSN: 0928-4931
PubMed: 26478304
WoS: 000364247500025
Scopus: 2-s2.0-84940556078
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Fanovich, M. A. AU - Ivanović, J. AU - Žižović, Irena AU - Mišić, Dušan AU - Jaeger, Philip PY - 2016 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1369 AB - Investigation of an integrated supercritical fluid extraction and supercritical solvent impregnation process for fabrication of microporous polycaprolactone-hydroxyapatite (PCL-HA) scaffolds with antibacterial activity is presented. The HA content and particle size as well as the operating conditions of the integrated process is optimized regarding the amount of impregnated antibacterial agent (Usnea lethariiformis extract) in the PCL-HA matrix, scaffold morphology and antibacterial activity against methicillin resistant Staphylococcus aureus (MRSA) strains. High pressure differential scanning calorimetry (HP-DSC) assay reveals that an increasing amount of HA results in decreasing melting temperature as well as crystallinity at an operating pressure of 17 MPa. The PCL-HA composites with micrometric sizes of the HA particles are convenient for being processed by the integrated process due to the simple preparation, a good interaction between the PCL matrix and filler and the advantageous impact on sorption. The scaffold obtained from PCL-HA with 20% of the HA shows the highest impregnation yield at 17 MPa and 35 degrees C (5.9%) and subsequently also the best bactericidal effect on the tested MRSA strains at an initial bacterial inoculum of 2 x 10(-4) CFU/mL PB - Elsevier Science Bv, Amsterdam T2 - Materials Science & Engineering C-Materials for Biological Applications T1 - Functionalization of polycaprolactone/hydroxyapatite scaffolds with Usnea lethariiformis extract by using supercritical CO2 VL - 58 SP - 204 EP - 212 DO - 10.1016/j.msec.2015.08.024 ER -
@article{ author = "Fanovich, M. A. and Ivanović, J. and Žižović, Irena and Mišić, Dušan and Jaeger, Philip", year = "2016", abstract = "Investigation of an integrated supercritical fluid extraction and supercritical solvent impregnation process for fabrication of microporous polycaprolactone-hydroxyapatite (PCL-HA) scaffolds with antibacterial activity is presented. The HA content and particle size as well as the operating conditions of the integrated process is optimized regarding the amount of impregnated antibacterial agent (Usnea lethariiformis extract) in the PCL-HA matrix, scaffold morphology and antibacterial activity against methicillin resistant Staphylococcus aureus (MRSA) strains. High pressure differential scanning calorimetry (HP-DSC) assay reveals that an increasing amount of HA results in decreasing melting temperature as well as crystallinity at an operating pressure of 17 MPa. The PCL-HA composites with micrometric sizes of the HA particles are convenient for being processed by the integrated process due to the simple preparation, a good interaction between the PCL matrix and filler and the advantageous impact on sorption. The scaffold obtained from PCL-HA with 20% of the HA shows the highest impregnation yield at 17 MPa and 35 degrees C (5.9%) and subsequently also the best bactericidal effect on the tested MRSA strains at an initial bacterial inoculum of 2 x 10(-4) CFU/mL", publisher = "Elsevier Science Bv, Amsterdam", journal = "Materials Science & Engineering C-Materials for Biological Applications", title = "Functionalization of polycaprolactone/hydroxyapatite scaffolds with Usnea lethariiformis extract by using supercritical CO2", volume = "58", pages = "204-212", doi = "10.1016/j.msec.2015.08.024" }
Fanovich, M. A., Ivanović, J., Žižović, I., Mišić, D.,& Jaeger, P.. (2016). Functionalization of polycaprolactone/hydroxyapatite scaffolds with Usnea lethariiformis extract by using supercritical CO2. in Materials Science & Engineering C-Materials for Biological Applications Elsevier Science Bv, Amsterdam., 58, 204-212. https://doi.org/10.1016/j.msec.2015.08.024
Fanovich MA, Ivanović J, Žižović I, Mišić D, Jaeger P. Functionalization of polycaprolactone/hydroxyapatite scaffolds with Usnea lethariiformis extract by using supercritical CO2. in Materials Science & Engineering C-Materials for Biological Applications. 2016;58:204-212. doi:10.1016/j.msec.2015.08.024 .
Fanovich, M. A., Ivanović, J., Žižović, Irena, Mišić, Dušan, Jaeger, Philip, "Functionalization of polycaprolactone/hydroxyapatite scaffolds with Usnea lethariiformis extract by using supercritical CO2" in Materials Science & Engineering C-Materials for Biological Applications, 58 (2016):204-212, https://doi.org/10.1016/j.msec.2015.08.024 . .