Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers
2017
Аутори
Kovacić, MarijanaMarković, Dragana
Maslovarić, Irina
Obrenović, Sonja
Grujic-Milanović, Jelica
Arsić, Aleksandra
Milanović, Zorana
Savić, Olivera
Fratrić, Natalija
Ilić, Vesna
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Calf bronchopneumonia is complex multifactorial disease and for its accurate diagnosis and therapy, besides clinical examination, microbiologic, hematologic and biochemical analyses could be necessary. In general, additional analyses are not implemented, mainly because the disease biomarkers are not defined. To establish which analysis might be useful for determining the severity of the disease, we analyzed 23 three-month old calves with mild clinical signs of bronchopneumonia and 15 age-matched healthy calves. Pasteurella multocida was isolated from deep nasal swabs of diseased calves. Peripheral blood erythrocyte and leukocyte count of bronchopneumonic and healthy calves showed no difference. Serum proteins, lipoproteins and lipids were analyzed with spectrophotometry, agarose gel electrophoresis, non-reducing SDSPAGE, gel zymography, and thin-layer chromatography. The bronchopneumonic calves had an increased level of circulating immune complexes and a globulins, which contain some o...f the positive acute phase proteins. In diseased calves the increased concentration of total. globulins (IgG), due to an increased concentration of anionic. globulins (predominately IgG1), was detected. The increased concentration of anionic. globulins followed by increased concentration of transferrin (negative acute phase protein) and HDL cholesterol, decreased concentration of LDL-cholesterol, unchanged activity of matrix metalloproteases and leukocyte counts might reflect the obvious absence of generalized inflammation. A positive correlation was found between the acquired results and the appearance of mild clinical signs. Therefore, we believe that the parameters analyzed in the peripheral blood could be applied as reliable disease markers to distinguish between severe (inflammatory) and mild forms of calf bronchopneumonia and to predict a better outcome for these calves.
Кључне речи:
biomarkers / calf bronchopneumonia / serum lipids / serum proteinsИзвор:
Acta Veterinaria-Beograd, 2017, 67, 2, 207-227Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
- Регенеративни и модулаторни потенцијал адултних матичних ћелија (RS-MESTD-Basic Research (BR or ON)-175062)
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
- Испитивање патогенезе хематолошких малигнитета (RS-MESTD-Basic Research (BR or ON)-175053)
DOI: 10.1515/acve-2017-0018
ISSN: 0567-8315
WoS: 000406311800006
Scopus: 2-s2.0-85021394474
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Kovacić, Marijana AU - Marković, Dragana AU - Maslovarić, Irina AU - Obrenović, Sonja AU - Grujic-Milanović, Jelica AU - Arsić, Aleksandra AU - Milanović, Zorana AU - Savić, Olivera AU - Fratrić, Natalija AU - Ilić, Vesna PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1478 AB - Calf bronchopneumonia is complex multifactorial disease and for its accurate diagnosis and therapy, besides clinical examination, microbiologic, hematologic and biochemical analyses could be necessary. In general, additional analyses are not implemented, mainly because the disease biomarkers are not defined. To establish which analysis might be useful for determining the severity of the disease, we analyzed 23 three-month old calves with mild clinical signs of bronchopneumonia and 15 age-matched healthy calves. Pasteurella multocida was isolated from deep nasal swabs of diseased calves. Peripheral blood erythrocyte and leukocyte count of bronchopneumonic and healthy calves showed no difference. Serum proteins, lipoproteins and lipids were analyzed with spectrophotometry, agarose gel electrophoresis, non-reducing SDSPAGE, gel zymography, and thin-layer chromatography. The bronchopneumonic calves had an increased level of circulating immune complexes and a globulins, which contain some of the positive acute phase proteins. In diseased calves the increased concentration of total. globulins (IgG), due to an increased concentration of anionic. globulins (predominately IgG1), was detected. The increased concentration of anionic. globulins followed by increased concentration of transferrin (negative acute phase protein) and HDL cholesterol, decreased concentration of LDL-cholesterol, unchanged activity of matrix metalloproteases and leukocyte counts might reflect the obvious absence of generalized inflammation. A positive correlation was found between the acquired results and the appearance of mild clinical signs. Therefore, we believe that the parameters analyzed in the peripheral blood could be applied as reliable disease markers to distinguish between severe (inflammatory) and mild forms of calf bronchopneumonia and to predict a better outcome for these calves. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers VL - 67 IS - 2 SP - 207 EP - 227 DO - 10.1515/acve-2017-0018 ER -
@article{ author = "Kovacić, Marijana and Marković, Dragana and Maslovarić, Irina and Obrenović, Sonja and Grujic-Milanović, Jelica and Arsić, Aleksandra and Milanović, Zorana and Savić, Olivera and Fratrić, Natalija and Ilić, Vesna", year = "2017", abstract = "Calf bronchopneumonia is complex multifactorial disease and for its accurate diagnosis and therapy, besides clinical examination, microbiologic, hematologic and biochemical analyses could be necessary. In general, additional analyses are not implemented, mainly because the disease biomarkers are not defined. To establish which analysis might be useful for determining the severity of the disease, we analyzed 23 three-month old calves with mild clinical signs of bronchopneumonia and 15 age-matched healthy calves. Pasteurella multocida was isolated from deep nasal swabs of diseased calves. Peripheral blood erythrocyte and leukocyte count of bronchopneumonic and healthy calves showed no difference. Serum proteins, lipoproteins and lipids were analyzed with spectrophotometry, agarose gel electrophoresis, non-reducing SDSPAGE, gel zymography, and thin-layer chromatography. The bronchopneumonic calves had an increased level of circulating immune complexes and a globulins, which contain some of the positive acute phase proteins. In diseased calves the increased concentration of total. globulins (IgG), due to an increased concentration of anionic. globulins (predominately IgG1), was detected. The increased concentration of anionic. globulins followed by increased concentration of transferrin (negative acute phase protein) and HDL cholesterol, decreased concentration of LDL-cholesterol, unchanged activity of matrix metalloproteases and leukocyte counts might reflect the obvious absence of generalized inflammation. A positive correlation was found between the acquired results and the appearance of mild clinical signs. Therefore, we believe that the parameters analyzed in the peripheral blood could be applied as reliable disease markers to distinguish between severe (inflammatory) and mild forms of calf bronchopneumonia and to predict a better outcome for these calves.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers", volume = "67", number = "2", pages = "207-227", doi = "10.1515/acve-2017-0018" }
Kovacić, M., Marković, D., Maslovarić, I., Obrenović, S., Grujic-Milanović, J., Arsić, A., Milanović, Z., Savić, O., Fratrić, N.,& Ilić, V.. (2017). Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(2), 207-227. https://doi.org/10.1515/acve-2017-0018
Kovacić M, Marković D, Maslovarić I, Obrenović S, Grujic-Milanović J, Arsić A, Milanović Z, Savić O, Fratrić N, Ilić V. Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers. in Acta Veterinaria-Beograd. 2017;67(2):207-227. doi:10.1515/acve-2017-0018 .
Kovacić, Marijana, Marković, Dragana, Maslovarić, Irina, Obrenović, Sonja, Grujic-Milanović, Jelica, Arsić, Aleksandra, Milanović, Zorana, Savić, Olivera, Fratrić, Natalija, Ilić, Vesna, "Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers" in Acta Veterinaria-Beograd, 67, no. 2 (2017):207-227, https://doi.org/10.1515/acve-2017-0018 . .