Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees
Само за регистроване кориснике
2017
Аутори
Orcić, SnežanaNikolić, Tatjana
Purac, Jelena
Sikoparija, Branko
Blagojević, Duško P.
Vukasinović, Elvira
Plavsa, Nada
Stevanović, Jevrosima
Kojić, Danijela
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The recent decline in managed honey bee populations, Apis mellifera L. (Hymenoptera: Apidae), has caused scientific, ecological, and economic concern. Research into the formation of reactive oxygen species (ROS), antioxidative defense mechanisms, and oxidative stress can contribute to our understanding of bee survival and conservation of this species. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) enzymes together with levels of malondialdehyde (MDA) were measured in summer and winter honey bees sampled from three colonies. One colony was stationary (C1), entering the winter period having accumulated Robinia pseudoacacia L. (Fabaceae) honey, and two were migratory (C2 and C3), entering the winter period with mainly Tilia (Malvaceae) and Brassica (Brassicaceae) honey, respectively. Compared to summer workers, winter worker bees had decreased SOD and GST activity, and MDA level, whereas CAT activity increased in all three colonies. We also d...emonstrated that seasonality is the main factor responsible for changes in antioxidant enzymes and MDA levels in worker honey bees. Overall, our results indicate a difference between summer and winter worker bees, pointing at a reduced level of antioxidant enzyme defenses during overwintering which may be due to a decrease in production of ROS. The decreased levels of MDA measured in winter honey bees confirm this. As ROS are actively used by insects as a defense mechanism to fight pathogens, we suggest that reduced production of ROS contributes to higher susceptibility of winter honey bees to infections and reduced overwinter survival.
Кључне речи:
oxidative stress / superoxide dismutase / catalase / glutathione S-transferase / floral composition of honey / melyssopalinology / Apis mellifera / Hymenoptera / ApidaeИзвор:
Entomologia Experimentalis et Applicata, 2017, 165, 2-3, 120-128Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Молекуларни механизми редокс сигналинга у хомеостази, адаптацији и патологији (RS-MESTD-Basic Research (BR or ON)-173014)
DOI: 10.1111/eea.12633
ISSN: 0013-8703
WoS: 000419907700002
Scopus: 2-s2.0-85040747078
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Orcić, Snežana AU - Nikolić, Tatjana AU - Purac, Jelena AU - Sikoparija, Branko AU - Blagojević, Duško P. AU - Vukasinović, Elvira AU - Plavsa, Nada AU - Stevanović, Jevrosima AU - Kojić, Danijela PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1537 AB - The recent decline in managed honey bee populations, Apis mellifera L. (Hymenoptera: Apidae), has caused scientific, ecological, and economic concern. Research into the formation of reactive oxygen species (ROS), antioxidative defense mechanisms, and oxidative stress can contribute to our understanding of bee survival and conservation of this species. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) enzymes together with levels of malondialdehyde (MDA) were measured in summer and winter honey bees sampled from three colonies. One colony was stationary (C1), entering the winter period having accumulated Robinia pseudoacacia L. (Fabaceae) honey, and two were migratory (C2 and C3), entering the winter period with mainly Tilia (Malvaceae) and Brassica (Brassicaceae) honey, respectively. Compared to summer workers, winter worker bees had decreased SOD and GST activity, and MDA level, whereas CAT activity increased in all three colonies. We also demonstrated that seasonality is the main factor responsible for changes in antioxidant enzymes and MDA levels in worker honey bees. Overall, our results indicate a difference between summer and winter worker bees, pointing at a reduced level of antioxidant enzyme defenses during overwintering which may be due to a decrease in production of ROS. The decreased levels of MDA measured in winter honey bees confirm this. As ROS are actively used by insects as a defense mechanism to fight pathogens, we suggest that reduced production of ROS contributes to higher susceptibility of winter honey bees to infections and reduced overwinter survival. PB - Wiley, Hoboken T2 - Entomologia Experimentalis et Applicata T1 - Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees VL - 165 IS - 2-3 SP - 120 EP - 128 DO - 10.1111/eea.12633 ER -
@article{ author = "Orcić, Snežana and Nikolić, Tatjana and Purac, Jelena and Sikoparija, Branko and Blagojević, Duško P. and Vukasinović, Elvira and Plavsa, Nada and Stevanović, Jevrosima and Kojić, Danijela", year = "2017", abstract = "The recent decline in managed honey bee populations, Apis mellifera L. (Hymenoptera: Apidae), has caused scientific, ecological, and economic concern. Research into the formation of reactive oxygen species (ROS), antioxidative defense mechanisms, and oxidative stress can contribute to our understanding of bee survival and conservation of this species. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) enzymes together with levels of malondialdehyde (MDA) were measured in summer and winter honey bees sampled from three colonies. One colony was stationary (C1), entering the winter period having accumulated Robinia pseudoacacia L. (Fabaceae) honey, and two were migratory (C2 and C3), entering the winter period with mainly Tilia (Malvaceae) and Brassica (Brassicaceae) honey, respectively. Compared to summer workers, winter worker bees had decreased SOD and GST activity, and MDA level, whereas CAT activity increased in all three colonies. We also demonstrated that seasonality is the main factor responsible for changes in antioxidant enzymes and MDA levels in worker honey bees. Overall, our results indicate a difference between summer and winter worker bees, pointing at a reduced level of antioxidant enzyme defenses during overwintering which may be due to a decrease in production of ROS. The decreased levels of MDA measured in winter honey bees confirm this. As ROS are actively used by insects as a defense mechanism to fight pathogens, we suggest that reduced production of ROS contributes to higher susceptibility of winter honey bees to infections and reduced overwinter survival.", publisher = "Wiley, Hoboken", journal = "Entomologia Experimentalis et Applicata", title = "Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees", volume = "165", number = "2-3", pages = "120-128", doi = "10.1111/eea.12633" }
Orcić, S., Nikolić, T., Purac, J., Sikoparija, B., Blagojević, D. P., Vukasinović, E., Plavsa, N., Stevanović, J.,& Kojić, D.. (2017). Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees. in Entomologia Experimentalis et Applicata Wiley, Hoboken., 165(2-3), 120-128. https://doi.org/10.1111/eea.12633
Orcić S, Nikolić T, Purac J, Sikoparija B, Blagojević DP, Vukasinović E, Plavsa N, Stevanović J, Kojić D. Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees. in Entomologia Experimentalis et Applicata. 2017;165(2-3):120-128. doi:10.1111/eea.12633 .
Orcić, Snežana, Nikolić, Tatjana, Purac, Jelena, Sikoparija, Branko, Blagojević, Duško P., Vukasinović, Elvira, Plavsa, Nada, Stevanović, Jevrosima, Kojić, Danijela, "Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees" in Entomologia Experimentalis et Applicata, 165, no. 2-3 (2017):120-128, https://doi.org/10.1111/eea.12633 . .