Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves
Само за регистроване кориснике
2017
Аутори
Jovanović, LjubomirPantelić, Marija
Prodanović, Radiša
Vujanac, Ivan
Đurić, Miloje
Tepavcević, Snežana
Vranješ-Đurić, Sanja
Korićanac, Goran
Kirovski, Danijela
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The objective of this study was to investigate the effects of peroral administration of chromium-enriched yeast on glucose tolerance in Holstein calves, assessed by insulin signaling pathway molecule determination and intravenous glucose tolerance test (IVGTT). Twenty-four Holstein calves, aged 1 month, were chosen for the study and divided into two groups: the PoCr group (n = 12) that perorally received 0.04 mg of Cr/kg of body mass daily, for 70 days, and the NCr group (n = 12) that received no chromium supplementation. Skeletal tissue samples from each calf were obtained on day 0 and day 70 of the experiment. Chromium supplementation increased protein content of the insulin beta-subunit receptor, phosphorylation of insulin receptor substrate 1 at Tyrosine 632, phosphorylation of Akt at Serine 473, glucose transporter-4, and AMP-activated protein kinase in skeletal muscle tissue, while phosphorylation of insulin receptor substrate 1 at Serine 307 was not affected by chromium treatmen...t. Results obtained during IVGTT, which was conducted on days 0, 30, 50, and 70, suggested an increased insulin sensitivity and, consequently, a better utilization of glucose in the PoCr group. Lower basal concentrations of glucose and insulin in the PoCr group on days 30 and 70 were also obtained. Our results indicate that chromium supplementation improves glucose utilization in calves by enhancing insulin intracellular signaling in the skeletal muscle tissue.
Кључне речи:
Calves / Chromium / Insulin / Signaling pathway / AMP-activated protein kinaseИзвор:
Biological Trace Element Research, 2017, 180, 2, 223-232Издавач:
- Humana Press Inc, Totowa
Финансирање / пројекти:
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
- Улога стероидних хормона у неуроендокриној адаптацији на стрес и патофизиологији метаболичког синдрома - молекуларни механизми и клиничке импликације (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
- Дефинисање кластера молекулских биомаркера за побољшану дијагностику и терапију поремећаја расположења (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41029)
DOI: 10.1007/s12011-017-1007-1
ISSN: 0163-4984
PubMed: 28378114
WoS: 000414196600006
Scopus: 2-s2.0-85017180340
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Jovanović, Ljubomir AU - Pantelić, Marija AU - Prodanović, Radiša AU - Vujanac, Ivan AU - Đurić, Miloje AU - Tepavcević, Snežana AU - Vranješ-Đurić, Sanja AU - Korićanac, Goran AU - Kirovski, Danijela PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1539 AB - The objective of this study was to investigate the effects of peroral administration of chromium-enriched yeast on glucose tolerance in Holstein calves, assessed by insulin signaling pathway molecule determination and intravenous glucose tolerance test (IVGTT). Twenty-four Holstein calves, aged 1 month, were chosen for the study and divided into two groups: the PoCr group (n = 12) that perorally received 0.04 mg of Cr/kg of body mass daily, for 70 days, and the NCr group (n = 12) that received no chromium supplementation. Skeletal tissue samples from each calf were obtained on day 0 and day 70 of the experiment. Chromium supplementation increased protein content of the insulin beta-subunit receptor, phosphorylation of insulin receptor substrate 1 at Tyrosine 632, phosphorylation of Akt at Serine 473, glucose transporter-4, and AMP-activated protein kinase in skeletal muscle tissue, while phosphorylation of insulin receptor substrate 1 at Serine 307 was not affected by chromium treatment. Results obtained during IVGTT, which was conducted on days 0, 30, 50, and 70, suggested an increased insulin sensitivity and, consequently, a better utilization of glucose in the PoCr group. Lower basal concentrations of glucose and insulin in the PoCr group on days 30 and 70 were also obtained. Our results indicate that chromium supplementation improves glucose utilization in calves by enhancing insulin intracellular signaling in the skeletal muscle tissue. PB - Humana Press Inc, Totowa T2 - Biological Trace Element Research T1 - Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves VL - 180 IS - 2 SP - 223 EP - 232 DO - 10.1007/s12011-017-1007-1 ER -
@article{ author = "Jovanović, Ljubomir and Pantelić, Marija and Prodanović, Radiša and Vujanac, Ivan and Đurić, Miloje and Tepavcević, Snežana and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela", year = "2017", abstract = "The objective of this study was to investigate the effects of peroral administration of chromium-enriched yeast on glucose tolerance in Holstein calves, assessed by insulin signaling pathway molecule determination and intravenous glucose tolerance test (IVGTT). Twenty-four Holstein calves, aged 1 month, were chosen for the study and divided into two groups: the PoCr group (n = 12) that perorally received 0.04 mg of Cr/kg of body mass daily, for 70 days, and the NCr group (n = 12) that received no chromium supplementation. Skeletal tissue samples from each calf were obtained on day 0 and day 70 of the experiment. Chromium supplementation increased protein content of the insulin beta-subunit receptor, phosphorylation of insulin receptor substrate 1 at Tyrosine 632, phosphorylation of Akt at Serine 473, glucose transporter-4, and AMP-activated protein kinase in skeletal muscle tissue, while phosphorylation of insulin receptor substrate 1 at Serine 307 was not affected by chromium treatment. Results obtained during IVGTT, which was conducted on days 0, 30, 50, and 70, suggested an increased insulin sensitivity and, consequently, a better utilization of glucose in the PoCr group. Lower basal concentrations of glucose and insulin in the PoCr group on days 30 and 70 were also obtained. Our results indicate that chromium supplementation improves glucose utilization in calves by enhancing insulin intracellular signaling in the skeletal muscle tissue.", publisher = "Humana Press Inc, Totowa", journal = "Biological Trace Element Research", title = "Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves", volume = "180", number = "2", pages = "223-232", doi = "10.1007/s12011-017-1007-1" }
Jovanović, L., Pantelić, M., Prodanović, R., Vujanac, I., Đurić, M., Tepavcević, S., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D.. (2017). Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves. in Biological Trace Element Research Humana Press Inc, Totowa., 180(2), 223-232. https://doi.org/10.1007/s12011-017-1007-1
Jovanović L, Pantelić M, Prodanović R, Vujanac I, Đurić M, Tepavcević S, Vranješ-Đurić S, Korićanac G, Kirovski D. Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves. in Biological Trace Element Research. 2017;180(2):223-232. doi:10.1007/s12011-017-1007-1 .
Jovanović, Ljubomir, Pantelić, Marija, Prodanović, Radiša, Vujanac, Ivan, Đurić, Miloje, Tepavcević, Snežana, Vranješ-Đurić, Sanja, Korićanac, Goran, Kirovski, Danijela, "Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves" in Biological Trace Element Research, 180, no. 2 (2017):223-232, https://doi.org/10.1007/s12011-017-1007-1 . .