Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro
2017
Autori
Bošnjak-Neumuller, JasnaĐelić, Ninoslav
Radaković, Milena
Kolarević, Stoimir
Mitić-Ćulafić, Dragana
Dajic-Stevanović, Zora
Vukovic-Gacić, Branka
Knežević-Vukčević, Jelena
Stanimirović, Zoran
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
There is increasing evidence that substances which are normally present in human or animal bodies may, under the certain circumstances, exhibit deleterious effects on genetic material, therefore acting as endogenous mutagenic agents. Since hormones represent one of the best studied endogenous mutagens, some research focused on the possible role of thyroid hormone in mutagenesis and carcinogenesis. Indeed, thyroid hormones accelerate aerobic metabolism and production of reactive oxygen species (ROS) and, therefore, may exhibit mutagenic effects in various test systems on mammalian cells. However, possible mutagenic effects on prokaryotic DNA has not been investigated so far. Hence, the aim of this research was to compare the sensitivity of TA 100 Salmonella typhimurium with and without metabolic activation with S9 fraction, and human lymphocytes to possible genotoxic effects of triiodothyronine (T-3). Therefore, we used the reverse mutation assay on S. typhimurium (Ames test) and in vit...ro Comet assay in isolated peripheral blood human lymphocytes. In both tests-systems a broad spectrum of T-3 concentrations was applied. The obtained results showed absence of genotoxic effects of T-3 in bacterial reverse mutation assay and very profound genotoxic effects in human lymphocytes at concentrations higher than 15 mu M. We only observed cytotoxic effects in bacterial system at very high T-3 concentrations (300 and 500 mu M). In conclusion, T-3 was unable to increase the level of reverse mutations in Ames test both with and without S9 mix. Therefore, it seems that ROS production in mitochondria may be the primary cause of DNA damage caused by T-3 in mammalian cells.
Ključne reči:
Comet assay / DNA damage / human lymphocytes / triiodothyronine / TA100 Salmonella typhimuriumIzvor:
Genetika, 2017, 49, 2, 387-397Izdavač:
- Društvo genetičara Srbije, Beograd
Finansiranje / projekti:
- Molekularno-genetička i ekofiziološka istraživanja u zaštiti autohtonih animalnih genetičkih resursa, očuvanju dobrobiti, zdravlja i reprodukcije gajenih životinja i proizvodnji bezbedne hrane (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.2298/GENSR1702387B
ISSN: 0534-0012
WoS: 000418533000002
Scopus: 2-s2.0-85033578412
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Bošnjak-Neumuller, Jasna AU - Đelić, Ninoslav AU - Radaković, Milena AU - Kolarević, Stoimir AU - Mitić-Ćulafić, Dragana AU - Dajic-Stevanović, Zora AU - Vukovic-Gacić, Branka AU - Knežević-Vukčević, Jelena AU - Stanimirović, Zoran PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1548 AB - There is increasing evidence that substances which are normally present in human or animal bodies may, under the certain circumstances, exhibit deleterious effects on genetic material, therefore acting as endogenous mutagenic agents. Since hormones represent one of the best studied endogenous mutagens, some research focused on the possible role of thyroid hormone in mutagenesis and carcinogenesis. Indeed, thyroid hormones accelerate aerobic metabolism and production of reactive oxygen species (ROS) and, therefore, may exhibit mutagenic effects in various test systems on mammalian cells. However, possible mutagenic effects on prokaryotic DNA has not been investigated so far. Hence, the aim of this research was to compare the sensitivity of TA 100 Salmonella typhimurium with and without metabolic activation with S9 fraction, and human lymphocytes to possible genotoxic effects of triiodothyronine (T-3). Therefore, we used the reverse mutation assay on S. typhimurium (Ames test) and in vitro Comet assay in isolated peripheral blood human lymphocytes. In both tests-systems a broad spectrum of T-3 concentrations was applied. The obtained results showed absence of genotoxic effects of T-3 in bacterial reverse mutation assay and very profound genotoxic effects in human lymphocytes at concentrations higher than 15 mu M. We only observed cytotoxic effects in bacterial system at very high T-3 concentrations (300 and 500 mu M). In conclusion, T-3 was unable to increase the level of reverse mutations in Ames test both with and without S9 mix. Therefore, it seems that ROS production in mitochondria may be the primary cause of DNA damage caused by T-3 in mammalian cells. PB - Društvo genetičara Srbije, Beograd T2 - Genetika T1 - Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro VL - 49 IS - 2 SP - 387 EP - 397 DO - 10.2298/GENSR1702387B ER -
@article{ author = "Bošnjak-Neumuller, Jasna and Đelić, Ninoslav and Radaković, Milena and Kolarević, Stoimir and Mitić-Ćulafić, Dragana and Dajic-Stevanović, Zora and Vukovic-Gacić, Branka and Knežević-Vukčević, Jelena and Stanimirović, Zoran", year = "2017", abstract = "There is increasing evidence that substances which are normally present in human or animal bodies may, under the certain circumstances, exhibit deleterious effects on genetic material, therefore acting as endogenous mutagenic agents. Since hormones represent one of the best studied endogenous mutagens, some research focused on the possible role of thyroid hormone in mutagenesis and carcinogenesis. Indeed, thyroid hormones accelerate aerobic metabolism and production of reactive oxygen species (ROS) and, therefore, may exhibit mutagenic effects in various test systems on mammalian cells. However, possible mutagenic effects on prokaryotic DNA has not been investigated so far. Hence, the aim of this research was to compare the sensitivity of TA 100 Salmonella typhimurium with and without metabolic activation with S9 fraction, and human lymphocytes to possible genotoxic effects of triiodothyronine (T-3). Therefore, we used the reverse mutation assay on S. typhimurium (Ames test) and in vitro Comet assay in isolated peripheral blood human lymphocytes. In both tests-systems a broad spectrum of T-3 concentrations was applied. The obtained results showed absence of genotoxic effects of T-3 in bacterial reverse mutation assay and very profound genotoxic effects in human lymphocytes at concentrations higher than 15 mu M. We only observed cytotoxic effects in bacterial system at very high T-3 concentrations (300 and 500 mu M). In conclusion, T-3 was unable to increase the level of reverse mutations in Ames test both with and without S9 mix. Therefore, it seems that ROS production in mitochondria may be the primary cause of DNA damage caused by T-3 in mammalian cells.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika", title = "Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro", volume = "49", number = "2", pages = "387-397", doi = "10.2298/GENSR1702387B" }
Bošnjak-Neumuller, J., Đelić, N., Radaković, M., Kolarević, S., Mitić-Ćulafić, D., Dajic-Stevanović, Z., Vukovic-Gacić, B., Knežević-Vukčević, J.,& Stanimirović, Z.. (2017). Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro. in Genetika Društvo genetičara Srbije, Beograd., 49(2), 387-397. https://doi.org/10.2298/GENSR1702387B
Bošnjak-Neumuller J, Đelić N, Radaković M, Kolarević S, Mitić-Ćulafić D, Dajic-Stevanović Z, Vukovic-Gacić B, Knežević-Vukčević J, Stanimirović Z. Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro. in Genetika. 2017;49(2):387-397. doi:10.2298/GENSR1702387B .
Bošnjak-Neumuller, Jasna, Đelić, Ninoslav, Radaković, Milena, Kolarević, Stoimir, Mitić-Ćulafić, Dragana, Dajic-Stevanović, Zora, Vukovic-Gacić, Branka, Knežević-Vukčević, Jelena, Stanimirović, Zoran, "Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro" in Genetika, 49, no. 2 (2017):387-397, https://doi.org/10.2298/GENSR1702387B . .