In vivo investigation of soft tissue response of novel silver/poly(vinyl alcohol)/ graphene and silver/poly(vinyl alcohol)/chitosan/graphene hydrogels aimed for medical applications - the first experience
2018
Аутори
Lužajić Božinovski, TijanaMarković, Danica
Todorović, Vera
Prokić, Bogomir Bolka
Milošević, Ivan
Drndarević, Neda
Nesović, Katarina
Kyong, Yop Rhee
Miskovic-Stanković, Vesna
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this paper, we have shown for the first time the soft tissue response of novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) and silver/poly(vinyl alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) nanocomposite hydrogels aimed for medical applications. These novel hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices as described in our previously published works. Both Ag/PVA/Gr and Ag/PVA/CHI/Gr, as well as controls Ag/PVA, Ag/PVA/CHI and commercial Suprasorb (c) hydrogel discs, were implanted in the subcutaneous tissue of rats. Implants with the surrounding tissue were dissected after post-implantation on days 7, 15, 30 and 60, and then processed for histological examination. The tissue irritation index (TIrI) score, according to ISO 10993-6, 2007, as well as the number of leukocytes in the peri-implant zone and connective tissue capsule thickness were examined. The results show that each TIrI score, the leukocyte number around the... implanted materials and capsule thickness gradually decreased during the observation period. At the endpoint of follow-up, the Ag/PVA/CHI/Gr implant was surrounded with a thinner capsule, while both the TIrI score and the number of leukocytes of the peri-implant zone were greater compared to the Ag/PVA/Gr implant. Despite the observed differences, we can conclude that our in vivo experiment suggested that both novel hydrogels were biocompatible and suitable for medical use.
Кључне речи:
biocompatibility / nanocomposite hydrogels / rat / soft tissue responseИзвор:
Acta Veterinaria-Beograd, 2018, 68, 3, 321-339Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
- Синтеза, развој технологија добијања и примена наноструктурних мултифункционалних материјала дефинисаних својстава (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45019)
- Basic Science Research Program through the National research Foundation of Korea - Ministry of Education, Science and Technology [2016R1A2B4016034]
DOI: 10.2478/acve-2018-0027
ISSN: 0567-8315
WoS: 000446384500007
Scopus: 2-s2.0-85056163172
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Lužajić Božinovski, Tijana AU - Marković, Danica AU - Todorović, Vera AU - Prokić, Bogomir Bolka AU - Milošević, Ivan AU - Drndarević, Neda AU - Nesović, Katarina AU - Kyong, Yop Rhee AU - Miskovic-Stanković, Vesna PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1587 AB - In this paper, we have shown for the first time the soft tissue response of novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) and silver/poly(vinyl alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) nanocomposite hydrogels aimed for medical applications. These novel hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices as described in our previously published works. Both Ag/PVA/Gr and Ag/PVA/CHI/Gr, as well as controls Ag/PVA, Ag/PVA/CHI and commercial Suprasorb (c) hydrogel discs, were implanted in the subcutaneous tissue of rats. Implants with the surrounding tissue were dissected after post-implantation on days 7, 15, 30 and 60, and then processed for histological examination. The tissue irritation index (TIrI) score, according to ISO 10993-6, 2007, as well as the number of leukocytes in the peri-implant zone and connective tissue capsule thickness were examined. The results show that each TIrI score, the leukocyte number around the implanted materials and capsule thickness gradually decreased during the observation period. At the endpoint of follow-up, the Ag/PVA/CHI/Gr implant was surrounded with a thinner capsule, while both the TIrI score and the number of leukocytes of the peri-implant zone were greater compared to the Ag/PVA/Gr implant. Despite the observed differences, we can conclude that our in vivo experiment suggested that both novel hydrogels were biocompatible and suitable for medical use. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - In vivo investigation of soft tissue response of novel silver/poly(vinyl alcohol)/ graphene and silver/poly(vinyl alcohol)/chitosan/graphene hydrogels aimed for medical applications - the first experience VL - 68 IS - 3 SP - 321 EP - 339 DO - 10.2478/acve-2018-0027 ER -
@article{ author = "Lužajić Božinovski, Tijana and Marković, Danica and Todorović, Vera and Prokić, Bogomir Bolka and Milošević, Ivan and Drndarević, Neda and Nesović, Katarina and Kyong, Yop Rhee and Miskovic-Stanković, Vesna", year = "2018", abstract = "In this paper, we have shown for the first time the soft tissue response of novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) and silver/poly(vinyl alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) nanocomposite hydrogels aimed for medical applications. These novel hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices as described in our previously published works. Both Ag/PVA/Gr and Ag/PVA/CHI/Gr, as well as controls Ag/PVA, Ag/PVA/CHI and commercial Suprasorb (c) hydrogel discs, were implanted in the subcutaneous tissue of rats. Implants with the surrounding tissue were dissected after post-implantation on days 7, 15, 30 and 60, and then processed for histological examination. The tissue irritation index (TIrI) score, according to ISO 10993-6, 2007, as well as the number of leukocytes in the peri-implant zone and connective tissue capsule thickness were examined. The results show that each TIrI score, the leukocyte number around the implanted materials and capsule thickness gradually decreased during the observation period. At the endpoint of follow-up, the Ag/PVA/CHI/Gr implant was surrounded with a thinner capsule, while both the TIrI score and the number of leukocytes of the peri-implant zone were greater compared to the Ag/PVA/Gr implant. Despite the observed differences, we can conclude that our in vivo experiment suggested that both novel hydrogels were biocompatible and suitable for medical use.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "In vivo investigation of soft tissue response of novel silver/poly(vinyl alcohol)/ graphene and silver/poly(vinyl alcohol)/chitosan/graphene hydrogels aimed for medical applications - the first experience", volume = "68", number = "3", pages = "321-339", doi = "10.2478/acve-2018-0027" }
Lužajić Božinovski, T., Marković, D., Todorović, V., Prokić, B. B., Milošević, I., Drndarević, N., Nesović, K., Kyong, Y. R.,& Miskovic-Stanković, V.. (2018). In vivo investigation of soft tissue response of novel silver/poly(vinyl alcohol)/ graphene and silver/poly(vinyl alcohol)/chitosan/graphene hydrogels aimed for medical applications - the first experience. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(3), 321-339. https://doi.org/10.2478/acve-2018-0027
Lužajić Božinovski T, Marković D, Todorović V, Prokić BB, Milošević I, Drndarević N, Nesović K, Kyong YR, Miskovic-Stanković V. In vivo investigation of soft tissue response of novel silver/poly(vinyl alcohol)/ graphene and silver/poly(vinyl alcohol)/chitosan/graphene hydrogels aimed for medical applications - the first experience. in Acta Veterinaria-Beograd. 2018;68(3):321-339. doi:10.2478/acve-2018-0027 .
Lužajić Božinovski, Tijana, Marković, Danica, Todorović, Vera, Prokić, Bogomir Bolka, Milošević, Ivan, Drndarević, Neda, Nesović, Katarina, Kyong, Yop Rhee, Miskovic-Stanković, Vesna, "In vivo investigation of soft tissue response of novel silver/poly(vinyl alcohol)/ graphene and silver/poly(vinyl alcohol)/chitosan/graphene hydrogels aimed for medical applications - the first experience" in Acta Veterinaria-Beograd, 68, no. 3 (2018):321-339, https://doi.org/10.2478/acve-2018-0027 . .