Quantitative PCR assessment of Lotmaria passim in Apis mellifera colonies co-infected naturally with Nosema ceranae
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2018
Authors
Vejnović, BranislavStevanović, Jevrosima
Schwarz, Ryan S.
Aleksić, Nevenka
Mirilović, Milorad
Jovanović, Nemanja M.
Stanimirović, Zoran
Article (Published version)
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A recently described trypanosomatid species Lotmaria passim and the microsporidium Nosema ceranae infect the honey bee (Apis mellifera), but the interspecific dynamic of these two common gut parasites is unknown. In this study, a real-time qPCR assay was developed to enable the specific detection and quantification of L. passim. The annual dynamics of N. ceranae and L. passim infections were evaluated in ten A. mellifera colonies naturally infected with both parasites at one apiary in Serbia from March 2016 to March 2017. Ten samples (60 bees abdomens) were taken from each colony on 8 sampling occasions. L. passim infection level was evaluated with qPCR, while N. ceranae infection was measured by spore counts. N. ceranae infection level was significantly higher in comparison with that of L. passim (spore or cell equivalents/bee, respectively). Significant positive correlation between infection levels of the parasite species indicates their similar annual dynamics, whilst the difference...s in the levels of infection between particular months point to a seasonal pattern in the incidence of both parasites. The assay which has been developed and validated creates opportunity for detailed study of L. passim infection kinetics and the improvement in the management practices in beekeeping related to these two parasites.
Keywords:
Apis mellifera / Co-infection / Interspecific dynamic / Lotmaria passim / Nosema ceranae / qPCRSource:
Journal of Invertebrate Pathology, 2018, 151, 76-81Publisher:
- Academic Press Inc Elsevier Science, San Diego
Funding / projects:
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.1016/j.jip.2017.11.003
ISSN: 0022-2011
PubMed: 29113738
WoS: 000424857300009
Scopus: 2-s2.0-85033564300
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Fakultet veterinarske medicineTY - JOUR AU - Vejnović, Branislav AU - Stevanović, Jevrosima AU - Schwarz, Ryan S. AU - Aleksić, Nevenka AU - Mirilović, Milorad AU - Jovanović, Nemanja M. AU - Stanimirović, Zoran PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1640 AB - A recently described trypanosomatid species Lotmaria passim and the microsporidium Nosema ceranae infect the honey bee (Apis mellifera), but the interspecific dynamic of these two common gut parasites is unknown. In this study, a real-time qPCR assay was developed to enable the specific detection and quantification of L. passim. The annual dynamics of N. ceranae and L. passim infections were evaluated in ten A. mellifera colonies naturally infected with both parasites at one apiary in Serbia from March 2016 to March 2017. Ten samples (60 bees abdomens) were taken from each colony on 8 sampling occasions. L. passim infection level was evaluated with qPCR, while N. ceranae infection was measured by spore counts. N. ceranae infection level was significantly higher in comparison with that of L. passim (spore or cell equivalents/bee, respectively). Significant positive correlation between infection levels of the parasite species indicates their similar annual dynamics, whilst the differences in the levels of infection between particular months point to a seasonal pattern in the incidence of both parasites. The assay which has been developed and validated creates opportunity for detailed study of L. passim infection kinetics and the improvement in the management practices in beekeeping related to these two parasites. PB - Academic Press Inc Elsevier Science, San Diego T2 - Journal of Invertebrate Pathology T1 - Quantitative PCR assessment of Lotmaria passim in Apis mellifera colonies co-infected naturally with Nosema ceranae VL - 151 SP - 76 EP - 81 DO - 10.1016/j.jip.2017.11.003 ER -
@article{ author = "Vejnović, Branislav and Stevanović, Jevrosima and Schwarz, Ryan S. and Aleksić, Nevenka and Mirilović, Milorad and Jovanović, Nemanja M. and Stanimirović, Zoran", year = "2018", abstract = "A recently described trypanosomatid species Lotmaria passim and the microsporidium Nosema ceranae infect the honey bee (Apis mellifera), but the interspecific dynamic of these two common gut parasites is unknown. In this study, a real-time qPCR assay was developed to enable the specific detection and quantification of L. passim. The annual dynamics of N. ceranae and L. passim infections were evaluated in ten A. mellifera colonies naturally infected with both parasites at one apiary in Serbia from March 2016 to March 2017. Ten samples (60 bees abdomens) were taken from each colony on 8 sampling occasions. L. passim infection level was evaluated with qPCR, while N. ceranae infection was measured by spore counts. N. ceranae infection level was significantly higher in comparison with that of L. passim (spore or cell equivalents/bee, respectively). Significant positive correlation between infection levels of the parasite species indicates their similar annual dynamics, whilst the differences in the levels of infection between particular months point to a seasonal pattern in the incidence of both parasites. The assay which has been developed and validated creates opportunity for detailed study of L. passim infection kinetics and the improvement in the management practices in beekeeping related to these two parasites.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Journal of Invertebrate Pathology", title = "Quantitative PCR assessment of Lotmaria passim in Apis mellifera colonies co-infected naturally with Nosema ceranae", volume = "151", pages = "76-81", doi = "10.1016/j.jip.2017.11.003" }
Vejnović, B., Stevanović, J., Schwarz, R. S., Aleksić, N., Mirilović, M., Jovanović, N. M.,& Stanimirović, Z.. (2018). Quantitative PCR assessment of Lotmaria passim in Apis mellifera colonies co-infected naturally with Nosema ceranae. in Journal of Invertebrate Pathology Academic Press Inc Elsevier Science, San Diego., 151, 76-81. https://doi.org/10.1016/j.jip.2017.11.003
Vejnović B, Stevanović J, Schwarz RS, Aleksić N, Mirilović M, Jovanović NM, Stanimirović Z. Quantitative PCR assessment of Lotmaria passim in Apis mellifera colonies co-infected naturally with Nosema ceranae. in Journal of Invertebrate Pathology. 2018;151:76-81. doi:10.1016/j.jip.2017.11.003 .
Vejnović, Branislav, Stevanović, Jevrosima, Schwarz, Ryan S., Aleksić, Nevenka, Mirilović, Milorad, Jovanović, Nemanja M., Stanimirović, Zoran, "Quantitative PCR assessment of Lotmaria passim in Apis mellifera colonies co-infected naturally with Nosema ceranae" in Journal of Invertebrate Pathology, 151 (2018):76-81, https://doi.org/10.1016/j.jip.2017.11.003 . .