Effects of agmatine on chlorpromazine-induced neuronal injury in rat
2018
Аутори
Dejanović, BratislavVukovic-Dejanović, Vesna
Ninković, Milica
Lavrnja, Irena
Stojanović, Ivana
Pavlović, Miloš
Begović, Vesna
Mirković, Dšsko
Stevanović, Ivana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This study was aimed to study the potentially beneficial effects of agmatine on oxidative/nitrosative stress development in the brain of Wistar rats during subacute chlorpromazine treatment. The animals were divided into control (0.9% saline), chlorpromazine (38.7 mg/kg b.w.), chlorpromazine+agmatine (agmatine 75 mg/kg b.w. immediately after chlorpromazine, 38.7 mg/ kg b.w. i.p.) and agmatine (75 mg/kg b.w.) groups. All the tested substances were administered intraperitoneally for 15 consecutive days and the rats were sacrificed by decapitation on day 15. Subacute administration of chlorpromazine resulted in increased lipid peroxidation, nitric oxide concentration and superoxide anion production, while completely damaging the antioxidant defence system in the cerebral cortex, striatum, and hippocampus. However, the combined treatment with chlorpromazine and agmatine significantly attenuated the oxidative/nitrosative stress indices and restored the antioxidant capacity to the control va...lues in all of the examined brain regions. Western blot analysis supported biochemical findings in all groups, but the most notable changes were found in the hippocampus. Our results suggest potentially beneficial effects of agmatine, which may be useful in the modified antioxidant approach in chlorpromazine-therapy.
Кључне речи:
Antioxidant defence / brain / oxidative stressИзвор:
Acta Veterinaria - Brno, 2018, 87, 2, 145-153Издавач:
- Veterinarni A Farmaceuticka Univerzita Brno, Brno
Финансирање / пројекти:
- Military Medical Academy [MPhiBMA1/18-20]
- Ћелијска и молекулска основа неуроинфламације: потенцијала циљна места за транслациону медицину и терапију (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41014)
DOI: 10.2754/avb201887020145
ISSN: 0001-7213
WoS: 000438898800007
Scopus: 2-s2.0-85049248429
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Dejanović, Bratislav AU - Vukovic-Dejanović, Vesna AU - Ninković, Milica AU - Lavrnja, Irena AU - Stojanović, Ivana AU - Pavlović, Miloš AU - Begović, Vesna AU - Mirković, Dšsko AU - Stevanović, Ivana PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1653 AB - This study was aimed to study the potentially beneficial effects of agmatine on oxidative/nitrosative stress development in the brain of Wistar rats during subacute chlorpromazine treatment. The animals were divided into control (0.9% saline), chlorpromazine (38.7 mg/kg b.w.), chlorpromazine+agmatine (agmatine 75 mg/kg b.w. immediately after chlorpromazine, 38.7 mg/ kg b.w. i.p.) and agmatine (75 mg/kg b.w.) groups. All the tested substances were administered intraperitoneally for 15 consecutive days and the rats were sacrificed by decapitation on day 15. Subacute administration of chlorpromazine resulted in increased lipid peroxidation, nitric oxide concentration and superoxide anion production, while completely damaging the antioxidant defence system in the cerebral cortex, striatum, and hippocampus. However, the combined treatment with chlorpromazine and agmatine significantly attenuated the oxidative/nitrosative stress indices and restored the antioxidant capacity to the control values in all of the examined brain regions. Western blot analysis supported biochemical findings in all groups, but the most notable changes were found in the hippocampus. Our results suggest potentially beneficial effects of agmatine, which may be useful in the modified antioxidant approach in chlorpromazine-therapy. PB - Veterinarni A Farmaceuticka Univerzita Brno, Brno T2 - Acta Veterinaria - Brno T1 - Effects of agmatine on chlorpromazine-induced neuronal injury in rat VL - 87 IS - 2 SP - 145 EP - 153 DO - 10.2754/avb201887020145 ER -
@article{ author = "Dejanović, Bratislav and Vukovic-Dejanović, Vesna and Ninković, Milica and Lavrnja, Irena and Stojanović, Ivana and Pavlović, Miloš and Begović, Vesna and Mirković, Dšsko and Stevanović, Ivana", year = "2018", abstract = "This study was aimed to study the potentially beneficial effects of agmatine on oxidative/nitrosative stress development in the brain of Wistar rats during subacute chlorpromazine treatment. The animals were divided into control (0.9% saline), chlorpromazine (38.7 mg/kg b.w.), chlorpromazine+agmatine (agmatine 75 mg/kg b.w. immediately after chlorpromazine, 38.7 mg/ kg b.w. i.p.) and agmatine (75 mg/kg b.w.) groups. All the tested substances were administered intraperitoneally for 15 consecutive days and the rats were sacrificed by decapitation on day 15. Subacute administration of chlorpromazine resulted in increased lipid peroxidation, nitric oxide concentration and superoxide anion production, while completely damaging the antioxidant defence system in the cerebral cortex, striatum, and hippocampus. However, the combined treatment with chlorpromazine and agmatine significantly attenuated the oxidative/nitrosative stress indices and restored the antioxidant capacity to the control values in all of the examined brain regions. Western blot analysis supported biochemical findings in all groups, but the most notable changes were found in the hippocampus. Our results suggest potentially beneficial effects of agmatine, which may be useful in the modified antioxidant approach in chlorpromazine-therapy.", publisher = "Veterinarni A Farmaceuticka Univerzita Brno, Brno", journal = "Acta Veterinaria - Brno", title = "Effects of agmatine on chlorpromazine-induced neuronal injury in rat", volume = "87", number = "2", pages = "145-153", doi = "10.2754/avb201887020145" }
Dejanović, B., Vukovic-Dejanović, V., Ninković, M., Lavrnja, I., Stojanović, I., Pavlović, M., Begović, V., Mirković, D.,& Stevanović, I.. (2018). Effects of agmatine on chlorpromazine-induced neuronal injury in rat. in Acta Veterinaria - Brno Veterinarni A Farmaceuticka Univerzita Brno, Brno., 87(2), 145-153. https://doi.org/10.2754/avb201887020145
Dejanović B, Vukovic-Dejanović V, Ninković M, Lavrnja I, Stojanović I, Pavlović M, Begović V, Mirković D, Stevanović I. Effects of agmatine on chlorpromazine-induced neuronal injury in rat. in Acta Veterinaria - Brno. 2018;87(2):145-153. doi:10.2754/avb201887020145 .
Dejanović, Bratislav, Vukovic-Dejanović, Vesna, Ninković, Milica, Lavrnja, Irena, Stojanović, Ivana, Pavlović, Miloš, Begović, Vesna, Mirković, Dšsko, Stevanović, Ivana, "Effects of agmatine on chlorpromazine-induced neuronal injury in rat" in Acta Veterinaria - Brno, 87, no. 2 (2018):145-153, https://doi.org/10.2754/avb201887020145 . .