Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia
2019
Authors
Vasić, AnaZdravković, Nemanja
Anita, Dragos
Bojkovski, Jovan
Marinov, Mihai
Mathis, Alexander
Niculaua, Marius
Oslobanu, Elena Luanda
Pavlović, Ivan
Petrić, Dusan
Pflueger, Valentin
Pudar, Dubravka
Savuta, Gheorghe
Simeunović, Predrag
Veronesi, Eva
Silaghi, Cornelia
Anita, Adriana
Anton, Ioana Alexandra
Cimpan, Andrei
Ciuca, Lavinia
Crivei, Luciana
Cojkić, Aleksandar
Davitkov, Darko
Drašković, Vladimir
Gajić, Bojan
Glavinić, Uroš
Ivanescu, Maria-Larisa
Kavran, Mihaela
Lupu, Andrei-Cristian
Mindru, Raluca
Porea, Daniela
Prodanović, Radiša
Radanović, Oliver
Raileanu, Cristian
Raileanu, Stefan
Ristanić, Marko
Roman, Constantin
Stanišić, Ljubodrag
Vaselek, Slavica
Đurić, Miloje
Article (Published version)
Metadata
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BackgroundCulicoides (Diptera: Ceratopogonidae) is a genus of small biting midges (also known as no-see ums) that currently includes 1368 described species. They are proven or suspected vectors for important pathogens affecting animals such as bluetongue virus (BTV) and Schmallenberg virus (SBV). Currently little information is available on the species of Culicoides present in Serbia. Thus, the aim of this study was to examine species diversity, host preference and the presence of BTV and SBV RNA in Culicoides from the Stara Planina Nature Park in south-eastern Serbia.ResultsIn total 19,887 individual Culicoides were collected during three nights of trapping at two farm sites and pooled into six groups (Obsoletus group, Pulicaris group, Others group and further each group according to the blood-feeding status to freshly engorged and non-engorged). Species identification was done on subsamples of 592 individual Culicoides specimens by morphological and molecular methods (MALDI-TOF mass ...spectrometry and PCR/sequencing). At least 22 Culicoides species were detected. Four animal species (cow, sheep, goat and common blackbird) as well as humans were identified as hosts of Culicoides biting midges. The screening of 8291 Culicoides specimens in 99 pools for the presence of BTV and SBV RNA by reverse-transcription quantitative PCR were negative.ConclusionsThe biodiversity of Culicoides species in the natural reserve Stara Planina was high with at least 22 species present. The presence of C. imicola Kieffer was not recorded in this area. Culicoides showed opportunistic feeding behaviour as determined by host preference. The absence of SBV and BTV viral RNA correlates with the absence of clinical disease in the field during the time of sampling. These data are the direct outcome of a training programme within the Institutional Partnership Project AMSAR: Arbovirus monitoring, research and surveillance-capacity building on mosquitoes and biting midges funded by the programme SCOPES of the Swiss National Science Foundation.
Keywords:
Culicoides spp / BTV / SBV / Host preference / Serbia / Capacity building / Train the trainers conceptSource:
Parasites & Vectors, 2019, 12, 61-Publisher:
- BMC, London
Funding / projects:
- SCOPES programme of the Swiss National Science Foundation (SNFS)
- Swiss Agency for Development and Cooperation (SDC)
DOI: 10.1186/s13071-019-3292-3
ISSN: 1756-3305
PubMed: 30683145
WoS: 000456777200001
Scopus: 2-s2.0-85060519002
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Vasić, Ana AU - Zdravković, Nemanja AU - Anita, Dragos AU - Bojkovski, Jovan AU - Marinov, Mihai AU - Mathis, Alexander AU - Niculaua, Marius AU - Oslobanu, Elena Luanda AU - Pavlović, Ivan AU - Petrić, Dusan AU - Pflueger, Valentin AU - Pudar, Dubravka AU - Savuta, Gheorghe AU - Simeunović, Predrag AU - Veronesi, Eva AU - Silaghi, Cornelia AU - Anita, Adriana AU - Anton, Ioana Alexandra AU - Cimpan, Andrei AU - Ciuca, Lavinia AU - Crivei, Luciana AU - Cojkić, Aleksandar AU - Davitkov, Darko AU - Drašković, Vladimir AU - Gajić, Bojan AU - Glavinić, Uroš AU - Ivanescu, Maria-Larisa AU - Kavran, Mihaela AU - Lupu, Andrei-Cristian AU - Mindru, Raluca AU - Porea, Daniela AU - Prodanović, Radiša AU - Radanović, Oliver AU - Raileanu, Cristian AU - Raileanu, Stefan AU - Ristanić, Marko AU - Roman, Constantin AU - Stanišić, Ljubodrag AU - Vaselek, Slavica AU - Đurić, Miloje PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1740 AB - BackgroundCulicoides (Diptera: Ceratopogonidae) is a genus of small biting midges (also known as no-see ums) that currently includes 1368 described species. They are proven or suspected vectors for important pathogens affecting animals such as bluetongue virus (BTV) and Schmallenberg virus (SBV). Currently little information is available on the species of Culicoides present in Serbia. Thus, the aim of this study was to examine species diversity, host preference and the presence of BTV and SBV RNA in Culicoides from the Stara Planina Nature Park in south-eastern Serbia.ResultsIn total 19,887 individual Culicoides were collected during three nights of trapping at two farm sites and pooled into six groups (Obsoletus group, Pulicaris group, Others group and further each group according to the blood-feeding status to freshly engorged and non-engorged). Species identification was done on subsamples of 592 individual Culicoides specimens by morphological and molecular methods (MALDI-TOF mass spectrometry and PCR/sequencing). At least 22 Culicoides species were detected. Four animal species (cow, sheep, goat and common blackbird) as well as humans were identified as hosts of Culicoides biting midges. The screening of 8291 Culicoides specimens in 99 pools for the presence of BTV and SBV RNA by reverse-transcription quantitative PCR were negative.ConclusionsThe biodiversity of Culicoides species in the natural reserve Stara Planina was high with at least 22 species present. The presence of C. imicola Kieffer was not recorded in this area. Culicoides showed opportunistic feeding behaviour as determined by host preference. The absence of SBV and BTV viral RNA correlates with the absence of clinical disease in the field during the time of sampling. These data are the direct outcome of a training programme within the Institutional Partnership Project AMSAR: Arbovirus monitoring, research and surveillance-capacity building on mosquitoes and biting midges funded by the programme SCOPES of the Swiss National Science Foundation. PB - BMC, London T2 - Parasites & Vectors T1 - Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia VL - 12 SP - 61 DO - 10.1186/s13071-019-3292-3 ER -
@article{ author = "Vasić, Ana and Zdravković, Nemanja and Anita, Dragos and Bojkovski, Jovan and Marinov, Mihai and Mathis, Alexander and Niculaua, Marius and Oslobanu, Elena Luanda and Pavlović, Ivan and Petrić, Dusan and Pflueger, Valentin and Pudar, Dubravka and Savuta, Gheorghe and Simeunović, Predrag and Veronesi, Eva and Silaghi, Cornelia and Anita, Adriana and Anton, Ioana Alexandra and Cimpan, Andrei and Ciuca, Lavinia and Crivei, Luciana and Cojkić, Aleksandar and Davitkov, Darko and Drašković, Vladimir and Gajić, Bojan and Glavinić, Uroš and Ivanescu, Maria-Larisa and Kavran, Mihaela and Lupu, Andrei-Cristian and Mindru, Raluca and Porea, Daniela and Prodanović, Radiša and Radanović, Oliver and Raileanu, Cristian and Raileanu, Stefan and Ristanić, Marko and Roman, Constantin and Stanišić, Ljubodrag and Vaselek, Slavica and Đurić, Miloje", year = "2019", abstract = "BackgroundCulicoides (Diptera: Ceratopogonidae) is a genus of small biting midges (also known as no-see ums) that currently includes 1368 described species. They are proven or suspected vectors for important pathogens affecting animals such as bluetongue virus (BTV) and Schmallenberg virus (SBV). Currently little information is available on the species of Culicoides present in Serbia. Thus, the aim of this study was to examine species diversity, host preference and the presence of BTV and SBV RNA in Culicoides from the Stara Planina Nature Park in south-eastern Serbia.ResultsIn total 19,887 individual Culicoides were collected during three nights of trapping at two farm sites and pooled into six groups (Obsoletus group, Pulicaris group, Others group and further each group according to the blood-feeding status to freshly engorged and non-engorged). Species identification was done on subsamples of 592 individual Culicoides specimens by morphological and molecular methods (MALDI-TOF mass spectrometry and PCR/sequencing). At least 22 Culicoides species were detected. Four animal species (cow, sheep, goat and common blackbird) as well as humans were identified as hosts of Culicoides biting midges. The screening of 8291 Culicoides specimens in 99 pools for the presence of BTV and SBV RNA by reverse-transcription quantitative PCR were negative.ConclusionsThe biodiversity of Culicoides species in the natural reserve Stara Planina was high with at least 22 species present. The presence of C. imicola Kieffer was not recorded in this area. Culicoides showed opportunistic feeding behaviour as determined by host preference. The absence of SBV and BTV viral RNA correlates with the absence of clinical disease in the field during the time of sampling. These data are the direct outcome of a training programme within the Institutional Partnership Project AMSAR: Arbovirus monitoring, research and surveillance-capacity building on mosquitoes and biting midges funded by the programme SCOPES of the Swiss National Science Foundation.", publisher = "BMC, London", journal = "Parasites & Vectors", title = "Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia", volume = "12", pages = "61", doi = "10.1186/s13071-019-3292-3" }
Vasić, A., Zdravković, N., Anita, D., Bojkovski, J., Marinov, M., Mathis, A., Niculaua, M., Oslobanu, E. L., Pavlović, I., Petrić, D., Pflueger, V., Pudar, D., Savuta, G., Simeunović, P., Veronesi, E., Silaghi, C., Anita, A., Anton, I. A., Cimpan, A., Ciuca, L., Crivei, L., Cojkić, A., Davitkov, D., Drašković, V., Gajić, B., Glavinić, U., Ivanescu, M., Kavran, M., Lupu, A., Mindru, R., Porea, D., Prodanović, R., Radanović, O., Raileanu, C., Raileanu, S., Ristanić, M., Roman, C., Stanišić, L., Vaselek, S.,& Đurić, M.. (2019). Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia. in Parasites & Vectors BMC, London., 12, 61. https://doi.org/10.1186/s13071-019-3292-3
Vasić A, Zdravković N, Anita D, Bojkovski J, Marinov M, Mathis A, Niculaua M, Oslobanu EL, Pavlović I, Petrić D, Pflueger V, Pudar D, Savuta G, Simeunović P, Veronesi E, Silaghi C, Anita A, Anton IA, Cimpan A, Ciuca L, Crivei L, Cojkić A, Davitkov D, Drašković V, Gajić B, Glavinić U, Ivanescu M, Kavran M, Lupu A, Mindru R, Porea D, Prodanović R, Radanović O, Raileanu C, Raileanu S, Ristanić M, Roman C, Stanišić L, Vaselek S, Đurić M. Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia. in Parasites & Vectors. 2019;12:61. doi:10.1186/s13071-019-3292-3 .
Vasić, Ana, Zdravković, Nemanja, Anita, Dragos, Bojkovski, Jovan, Marinov, Mihai, Mathis, Alexander, Niculaua, Marius, Oslobanu, Elena Luanda, Pavlović, Ivan, Petrić, Dusan, Pflueger, Valentin, Pudar, Dubravka, Savuta, Gheorghe, Simeunović, Predrag, Veronesi, Eva, Silaghi, Cornelia, Anita, Adriana, Anton, Ioana Alexandra, Cimpan, Andrei, Ciuca, Lavinia, Crivei, Luciana, Cojkić, Aleksandar, Davitkov, Darko, Drašković, Vladimir, Gajić, Bojan, Glavinić, Uroš, Ivanescu, Maria-Larisa, Kavran, Mihaela, Lupu, Andrei-Cristian, Mindru, Raluca, Porea, Daniela, Prodanović, Radiša, Radanović, Oliver, Raileanu, Cristian, Raileanu, Stefan, Ristanić, Marko, Roman, Constantin, Stanišić, Ljubodrag, Vaselek, Slavica, Đurić, Miloje, "Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia" in Parasites & Vectors, 12 (2019):61, https://doi.org/10.1186/s13071-019-3292-3 . .