Levamisole receptor phosphorylation: effects of kinase antagonists on membrane potential responses in Ascaris suum suggest that CaM kinase and tyrosine kinase regulate sensitivity to levamisole
Само за регистроване кориснике
2002
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A two-micropipette current-clamp technique was used
to record electrophysiological responses from the somatic
muscle of Ascaris suum. Levamisole and acetylcholine
were applied to the bag region of the muscle using a
microperfusion system. Depolarizations produced by 10 s
applications of 10 µmol l–1 levamisole or 20 s applications
of 10 µmol l–1 acetylcholine were recorded. The effect on
the peak membrane potential change of the kinase
antagonists H-7, staurosporine, KN-93 and genistein was
observed. H-7 (30 µmol l–1), a non-selective antagonist of
protein kinases A, C and G but which has little effect on
Ca2+/calmodulin-dependent kinase II (CaM kinase II), did
not produce a significant effect on the peak response to
levamisole or acetylcholine. Staurosporine (1 µmol l–1), a
non-selective kinase antagonist that has effects on protein
kinases A, C and G, CaM kinase and tyrosine kinase,
reduced the mean peak membrane potential response to
levamisole from 6.8 mV to 3.9 mV ...(P<0.0001) and the
mean response to acetylcholine from 5.5 mV to 2.8 mV
(P=0.0016). The difference between the effects of H-7 and
staurosporine suggested the involvement of CaM kinase II
and/or tyrosine kinase. KN-93, a selective CaM kinase II
antagonist, reduced the mean peak response to levamisole
from 6.2 mV to 2.7 mV (P=0.035) and the mean peak
response of acetylcholine from 4.7 mV to 2.0 mV
(P=0.0004). The effects indicated the involvement of CaM
kinase II in the phosphorylation of levamisole and
acetylcholine receptors. The effect of extracellular Ca2+ on
the response to levamisole was assessed by comparing
responses to levamisole in normal and in low-Ca2+ bathing
solutions. The response to levamisole was greater in the
presence of Ca2+, an effect that may be explained by
stimulation of CaM kinase II. Genistein (90 µmol l–1), a
selective tyrosine kinase antagonist, reduced peak
membrane potential responses to levamisole from a mean
of 6.4 mV to 3.3 mV (P=0.001). This effect indicated the
involvement of tyrosine kinase in maintaining the
receptor.
Кључне речи:
levamisole, kinase antagonist, H-7, KN-93, genistein, staurosporine, Ascaris summ, nematode, CaM kinase, tyrosine kinase, membrane potential response.Извор:
Journal of Experimental Biology, 2002, 205, 3979-3988Издавач:
- The Company of Biologists
Финансирање / пројекти:
- NIH to R.J.M.: RO1 A147194-02.
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Trailović, Saša AU - Robertson, Alan P. AU - Clark, Cheryl L. AU - Martin, Richard J. PY - 2002 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2624 AB - A two-micropipette current-clamp technique was used to record electrophysiological responses from the somatic muscle of Ascaris suum. Levamisole and acetylcholine were applied to the bag region of the muscle using a microperfusion system. Depolarizations produced by 10 s applications of 10 µmol l–1 levamisole or 20 s applications of 10 µmol l–1 acetylcholine were recorded. The effect on the peak membrane potential change of the kinase antagonists H-7, staurosporine, KN-93 and genistein was observed. H-7 (30 µmol l–1), a non-selective antagonist of protein kinases A, C and G but which has little effect on Ca2+/calmodulin-dependent kinase II (CaM kinase II), did not produce a significant effect on the peak response to levamisole or acetylcholine. Staurosporine (1 µmol l–1), a non-selective kinase antagonist that has effects on protein kinases A, C and G, CaM kinase and tyrosine kinase, reduced the mean peak membrane potential response to levamisole from 6.8 mV to 3.9 mV (P<0.0001) and the mean response to acetylcholine from 5.5 mV to 2.8 mV (P=0.0016). The difference between the effects of H-7 and staurosporine suggested the involvement of CaM kinase II and/or tyrosine kinase. KN-93, a selective CaM kinase II antagonist, reduced the mean peak response to levamisole from 6.2 mV to 2.7 mV (P=0.035) and the mean peak response of acetylcholine from 4.7 mV to 2.0 mV (P=0.0004). The effects indicated the involvement of CaM kinase II in the phosphorylation of levamisole and acetylcholine receptors. The effect of extracellular Ca2+ on the response to levamisole was assessed by comparing responses to levamisole in normal and in low-Ca2+ bathing solutions. The response to levamisole was greater in the presence of Ca2+, an effect that may be explained by stimulation of CaM kinase II. Genistein (90 µmol l–1), a selective tyrosine kinase antagonist, reduced peak membrane potential responses to levamisole from a mean of 6.4 mV to 3.3 mV (P=0.001). This effect indicated the involvement of tyrosine kinase in maintaining the receptor. PB - The Company of Biologists T2 - Journal of Experimental Biology T1 - Levamisole receptor phosphorylation: effects of kinase antagonists on membrane potential responses in Ascaris suum suggest that CaM kinase and tyrosine kinase regulate sensitivity to levamisole VL - 205 SP - 3979 EP - 3988 DO - 10.1242/jeb.205.24.3979 ER -
@article{ author = "Trailović, Saša and Robertson, Alan P. and Clark, Cheryl L. and Martin, Richard J.", year = "2002", abstract = "A two-micropipette current-clamp technique was used to record electrophysiological responses from the somatic muscle of Ascaris suum. Levamisole and acetylcholine were applied to the bag region of the muscle using a microperfusion system. Depolarizations produced by 10 s applications of 10 µmol l–1 levamisole or 20 s applications of 10 µmol l–1 acetylcholine were recorded. The effect on the peak membrane potential change of the kinase antagonists H-7, staurosporine, KN-93 and genistein was observed. H-7 (30 µmol l–1), a non-selective antagonist of protein kinases A, C and G but which has little effect on Ca2+/calmodulin-dependent kinase II (CaM kinase II), did not produce a significant effect on the peak response to levamisole or acetylcholine. Staurosporine (1 µmol l–1), a non-selective kinase antagonist that has effects on protein kinases A, C and G, CaM kinase and tyrosine kinase, reduced the mean peak membrane potential response to levamisole from 6.8 mV to 3.9 mV (P<0.0001) and the mean response to acetylcholine from 5.5 mV to 2.8 mV (P=0.0016). The difference between the effects of H-7 and staurosporine suggested the involvement of CaM kinase II and/or tyrosine kinase. KN-93, a selective CaM kinase II antagonist, reduced the mean peak response to levamisole from 6.2 mV to 2.7 mV (P=0.035) and the mean peak response of acetylcholine from 4.7 mV to 2.0 mV (P=0.0004). The effects indicated the involvement of CaM kinase II in the phosphorylation of levamisole and acetylcholine receptors. The effect of extracellular Ca2+ on the response to levamisole was assessed by comparing responses to levamisole in normal and in low-Ca2+ bathing solutions. The response to levamisole was greater in the presence of Ca2+, an effect that may be explained by stimulation of CaM kinase II. Genistein (90 µmol l–1), a selective tyrosine kinase antagonist, reduced peak membrane potential responses to levamisole from a mean of 6.4 mV to 3.3 mV (P=0.001). This effect indicated the involvement of tyrosine kinase in maintaining the receptor.", publisher = "The Company of Biologists", journal = "Journal of Experimental Biology", title = "Levamisole receptor phosphorylation: effects of kinase antagonists on membrane potential responses in Ascaris suum suggest that CaM kinase and tyrosine kinase regulate sensitivity to levamisole", volume = "205", pages = "3979-3988", doi = "10.1242/jeb.205.24.3979" }
Trailović, S., Robertson, A. P., Clark, C. L.,& Martin, R. J.. (2002). Levamisole receptor phosphorylation: effects of kinase antagonists on membrane potential responses in Ascaris suum suggest that CaM kinase and tyrosine kinase regulate sensitivity to levamisole. in Journal of Experimental Biology The Company of Biologists., 205, 3979-3988. https://doi.org/10.1242/jeb.205.24.3979
Trailović S, Robertson AP, Clark CL, Martin RJ. Levamisole receptor phosphorylation: effects of kinase antagonists on membrane potential responses in Ascaris suum suggest that CaM kinase and tyrosine kinase regulate sensitivity to levamisole. in Journal of Experimental Biology. 2002;205:3979-3988. doi:10.1242/jeb.205.24.3979 .
Trailović, Saša, Robertson, Alan P., Clark, Cheryl L., Martin, Richard J., "Levamisole receptor phosphorylation: effects of kinase antagonists on membrane potential responses in Ascaris suum suggest that CaM kinase and tyrosine kinase regulate sensitivity to levamisole" in Journal of Experimental Biology, 205 (2002):3979-3988, https://doi.org/10.1242/jeb.205.24.3979 . .
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