Examination of the activity of HN and F glycoprotein antigens of the outer envelope of Newcastle disease virus by using fusional, hemolytic, hemagglutination and hemadsorption tests, in vitro
Ispitivanje aktivnosti glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti primenom testova fuzije, hemolize, hemaglutinacije i hemadsorpcije, in vitro
Abstract
The objective of our study was to examine fusional, hemolytic, hemagglutination and hemadsorption activities of the surface glycoprotein HN and F antigens of Newcastle disease (ND) virus, in vitro. The samples of activated ND virions, induced Vero cell fusion after 6h, 12h, 24h and 48h. After 24h of treatment of the inoculated Vero cells with dilutions of the specific immune sera against ND virus, cell fusion was not registered at dilutions of 1:2 and 1:4. The ND virion samples, activated with 0.025 g/dL trypsin-versen, induced hemolysis of chicken erythrocytes at antigen dilutions of 1:4 and 1:8. The samples of activated ND virions, expressed an intensive hemagglutinating activity of 256 HAU/0,1 mL. After treatment of the abovementioned samples with specific immune sera against ND virus, hemolytic activities were not detected at immune sera dilution of 1:32. The hemadsorption of chicken erythrocytes at the surface of inoculated Vero cells was detected after 6h of inoculation with acti...vated ND viruses. After 24h of treatment of inoculated Vero cells with dilutions of specific immune sera against ND virus, the hemadsorption of chicken erythrocytes was not registered at immune sera dilution of 1:64. These results showed the possibility to use fusion, hemolytic and hemadsorption tests for the detection of immunologically important glycoprotein antigens of ND viruses and their identification with specific immune sera.
Cilj ovih istraživanja je bilo ispitivanje fuzionih, hemolitičkih, hemaglutinacionih i hemadsorpcionih aktivnosti glikoproteinskih antigena virusa Newcastle bolesti, in vitro. Rezultati testova ćelijske fuzije pokazali su da je virus Newcastle bolesti posle aktivacije sa 0,025 g/dl tripsin-versena indukovao fuziju Vero ćelija posle 6h, 12h, 24h i 48 od inokulacije virusa. Tretiranjem inokulisanih Vero ćelija sa razređenjima imunih seruma protiv virusa Newcastle bolesti, ustanovljena je inhibicija ćelijske fuzije u razređenjima seruma od 1:2 i 1:4. Uzorci virusa Newcastle bolesti, indukovali su posle aktivisanja sa 0,025 g/dl tripsin-versena, hemolizu eritrocita kokoši u razređenjima antigena od 1:4 do1:8. Isti uzorci su ispoljili i intenzivne hemaglutinacione aktivnosti posle aktivacije tripsin-versenom koje su iznosile 256 HJ/0,1 ml za virus Newcastle bolesti. Posle tretiranja uzoraka aktivisanih virusa Newcastle bolesti sa razređenjima specifičnih imunih seruma, nije ustanovljena nji...hova hemolitička aktivnost do razređenja seruma od 1:32. Posle 6h, 12h i 24h od inokulacije Vero ćelija aktivisanim uzorcima virusa Newcastle bolesti, ustanovljena je pojava hemadsorpcije eritrocita kokoši u različitim razređenjima antigena virusa. Posle 24h od tretiranja inokulisanih Vero ćelija sa razređenjima specifičnog imunog seruma protiv virusa Newcastle bolesti, nije ustanovljena po-java hemadsorpcije eritrocita kokoši do razređenja imunog seruma od 1:64. Ovi rezultati su ukazali na mogućnost korišćenja fuzionog, hemolitičnog i hemadsorpcionog testa za brzu detekciju imunološki značajnih glikoproteinskih antigena virusa Newcastle bolesti i njihovu identifikaciju primenom specifičnih imunih seruma.
Keywords:
Newcastle disease virus / fusion test / hemagglutination / hemolysis / hemadsorptionSource:
Acta Veterinaria-Beograd, 2007, 57, 1, 3-10Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
DOI: 10.2298/AVB0701003N
ISSN: 0567-8315
WoS: 000245603000001
Scopus: 2-s2.0-34247572339
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Fakultet veterinarske medicineTY - JOUR AU - Nišavić, Jakov AU - Milić, Nenad AU - Veljović, Ljubiša PY - 2007 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/479 AB - The objective of our study was to examine fusional, hemolytic, hemagglutination and hemadsorption activities of the surface glycoprotein HN and F antigens of Newcastle disease (ND) virus, in vitro. The samples of activated ND virions, induced Vero cell fusion after 6h, 12h, 24h and 48h. After 24h of treatment of the inoculated Vero cells with dilutions of the specific immune sera against ND virus, cell fusion was not registered at dilutions of 1:2 and 1:4. The ND virion samples, activated with 0.025 g/dL trypsin-versen, induced hemolysis of chicken erythrocytes at antigen dilutions of 1:4 and 1:8. The samples of activated ND virions, expressed an intensive hemagglutinating activity of 256 HAU/0,1 mL. After treatment of the abovementioned samples with specific immune sera against ND virus, hemolytic activities were not detected at immune sera dilution of 1:32. The hemadsorption of chicken erythrocytes at the surface of inoculated Vero cells was detected after 6h of inoculation with activated ND viruses. After 24h of treatment of inoculated Vero cells with dilutions of specific immune sera against ND virus, the hemadsorption of chicken erythrocytes was not registered at immune sera dilution of 1:64. These results showed the possibility to use fusion, hemolytic and hemadsorption tests for the detection of immunologically important glycoprotein antigens of ND viruses and their identification with specific immune sera. AB - Cilj ovih istraživanja je bilo ispitivanje fuzionih, hemolitičkih, hemaglutinacionih i hemadsorpcionih aktivnosti glikoproteinskih antigena virusa Newcastle bolesti, in vitro. Rezultati testova ćelijske fuzije pokazali su da je virus Newcastle bolesti posle aktivacije sa 0,025 g/dl tripsin-versena indukovao fuziju Vero ćelija posle 6h, 12h, 24h i 48 od inokulacije virusa. Tretiranjem inokulisanih Vero ćelija sa razređenjima imunih seruma protiv virusa Newcastle bolesti, ustanovljena je inhibicija ćelijske fuzije u razređenjima seruma od 1:2 i 1:4. Uzorci virusa Newcastle bolesti, indukovali su posle aktivisanja sa 0,025 g/dl tripsin-versena, hemolizu eritrocita kokoši u razređenjima antigena od 1:4 do1:8. Isti uzorci su ispoljili i intenzivne hemaglutinacione aktivnosti posle aktivacije tripsin-versenom koje su iznosile 256 HJ/0,1 ml za virus Newcastle bolesti. Posle tretiranja uzoraka aktivisanih virusa Newcastle bolesti sa razređenjima specifičnih imunih seruma, nije ustanovljena njihova hemolitička aktivnost do razređenja seruma od 1:32. Posle 6h, 12h i 24h od inokulacije Vero ćelija aktivisanim uzorcima virusa Newcastle bolesti, ustanovljena je pojava hemadsorpcije eritrocita kokoši u različitim razređenjima antigena virusa. Posle 24h od tretiranja inokulisanih Vero ćelija sa razređenjima specifičnog imunog seruma protiv virusa Newcastle bolesti, nije ustanovljena po-java hemadsorpcije eritrocita kokoši do razređenja imunog seruma od 1:64. Ovi rezultati su ukazali na mogućnost korišćenja fuzionog, hemolitičnog i hemadsorpcionog testa za brzu detekciju imunološki značajnih glikoproteinskih antigena virusa Newcastle bolesti i njihovu identifikaciju primenom specifičnih imunih seruma. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Examination of the activity of HN and F glycoprotein antigens of the outer envelope of Newcastle disease virus by using fusional, hemolytic, hemagglutination and hemadsorption tests, in vitro T1 - Ispitivanje aktivnosti glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti primenom testova fuzije, hemolize, hemaglutinacije i hemadsorpcije, in vitro VL - 57 IS - 1 SP - 3 EP - 10 DO - 10.2298/AVB0701003N ER -
@article{ author = "Nišavić, Jakov and Milić, Nenad and Veljović, Ljubiša", year = "2007", abstract = "The objective of our study was to examine fusional, hemolytic, hemagglutination and hemadsorption activities of the surface glycoprotein HN and F antigens of Newcastle disease (ND) virus, in vitro. The samples of activated ND virions, induced Vero cell fusion after 6h, 12h, 24h and 48h. After 24h of treatment of the inoculated Vero cells with dilutions of the specific immune sera against ND virus, cell fusion was not registered at dilutions of 1:2 and 1:4. The ND virion samples, activated with 0.025 g/dL trypsin-versen, induced hemolysis of chicken erythrocytes at antigen dilutions of 1:4 and 1:8. The samples of activated ND virions, expressed an intensive hemagglutinating activity of 256 HAU/0,1 mL. After treatment of the abovementioned samples with specific immune sera against ND virus, hemolytic activities were not detected at immune sera dilution of 1:32. The hemadsorption of chicken erythrocytes at the surface of inoculated Vero cells was detected after 6h of inoculation with activated ND viruses. After 24h of treatment of inoculated Vero cells with dilutions of specific immune sera against ND virus, the hemadsorption of chicken erythrocytes was not registered at immune sera dilution of 1:64. These results showed the possibility to use fusion, hemolytic and hemadsorption tests for the detection of immunologically important glycoprotein antigens of ND viruses and their identification with specific immune sera., Cilj ovih istraživanja je bilo ispitivanje fuzionih, hemolitičkih, hemaglutinacionih i hemadsorpcionih aktivnosti glikoproteinskih antigena virusa Newcastle bolesti, in vitro. Rezultati testova ćelijske fuzije pokazali su da je virus Newcastle bolesti posle aktivacije sa 0,025 g/dl tripsin-versena indukovao fuziju Vero ćelija posle 6h, 12h, 24h i 48 od inokulacije virusa. Tretiranjem inokulisanih Vero ćelija sa razređenjima imunih seruma protiv virusa Newcastle bolesti, ustanovljena je inhibicija ćelijske fuzije u razređenjima seruma od 1:2 i 1:4. Uzorci virusa Newcastle bolesti, indukovali su posle aktivisanja sa 0,025 g/dl tripsin-versena, hemolizu eritrocita kokoši u razređenjima antigena od 1:4 do1:8. Isti uzorci su ispoljili i intenzivne hemaglutinacione aktivnosti posle aktivacije tripsin-versenom koje su iznosile 256 HJ/0,1 ml za virus Newcastle bolesti. Posle tretiranja uzoraka aktivisanih virusa Newcastle bolesti sa razređenjima specifičnih imunih seruma, nije ustanovljena njihova hemolitička aktivnost do razređenja seruma od 1:32. Posle 6h, 12h i 24h od inokulacije Vero ćelija aktivisanim uzorcima virusa Newcastle bolesti, ustanovljena je pojava hemadsorpcije eritrocita kokoši u različitim razređenjima antigena virusa. Posle 24h od tretiranja inokulisanih Vero ćelija sa razređenjima specifičnog imunog seruma protiv virusa Newcastle bolesti, nije ustanovljena po-java hemadsorpcije eritrocita kokoši do razređenja imunog seruma od 1:64. Ovi rezultati su ukazali na mogućnost korišćenja fuzionog, hemolitičnog i hemadsorpcionog testa za brzu detekciju imunološki značajnih glikoproteinskih antigena virusa Newcastle bolesti i njihovu identifikaciju primenom specifičnih imunih seruma.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Examination of the activity of HN and F glycoprotein antigens of the outer envelope of Newcastle disease virus by using fusional, hemolytic, hemagglutination and hemadsorption tests, in vitro, Ispitivanje aktivnosti glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti primenom testova fuzije, hemolize, hemaglutinacije i hemadsorpcije, in vitro", volume = "57", number = "1", pages = "3-10", doi = "10.2298/AVB0701003N" }
Nišavić, J., Milić, N.,& Veljović, L.. (2007). Examination of the activity of HN and F glycoprotein antigens of the outer envelope of Newcastle disease virus by using fusional, hemolytic, hemagglutination and hemadsorption tests, in vitro. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(1), 3-10. https://doi.org/10.2298/AVB0701003N
Nišavić J, Milić N, Veljović L. Examination of the activity of HN and F glycoprotein antigens of the outer envelope of Newcastle disease virus by using fusional, hemolytic, hemagglutination and hemadsorption tests, in vitro. in Acta Veterinaria-Beograd. 2007;57(1):3-10. doi:10.2298/AVB0701003N .
Nišavić, Jakov, Milić, Nenad, Veljović, Ljubiša, "Examination of the activity of HN and F glycoprotein antigens of the outer envelope of Newcastle disease virus by using fusional, hemolytic, hemagglutination and hemadsorption tests, in vitro" in Acta Veterinaria-Beograd, 57, no. 1 (2007):3-10, https://doi.org/10.2298/AVB0701003N . .