The effect of a new nitro-aspirin on apoptosis of neutrophil granulocytes
Efekat novog nitro-aspirina na apoptozu neutrofilnih granulocita
2008
Autori
Vasilev, SašaVučević, Dragana
Gašić, Sonja
Majstorović, Ivana
Vasilijić, S.
Ćupić, Vitomir
Čolić, M.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Apoptosis of neutrophil granulocytes is a critical event in the resolution of inflammation. Neutrophils have a short lifespan which can be modulated by aspirin. In this work we studied the effect of a nitroaspirin (NCX4040) on apoptosis of inflammatory granulocytes. This nitro-aspirin has been synthesized in attempt to reduce the side effects of aspirin in the gastrointestinal tract. Inflammatory granulocytes have been isolated from polyvinyl sponges implanted under the skin of Albino Oxford (AO) rats. Inflammatory cells that were isolated 20 hours later were about 95% neutrophil granulocytes. The cells were cultivated 24h with different concentrations of NCX4040 ranging from 0.01 μM to 10μM. After that period, apoptosis of neutrophils was performed by using morphological criteria, as well as by flow cytometry (after staining the cells with propidium iodide). We found that NCX4040 at concentrations from 0.25 to 10 μM induced the apoptosis of rat inflammatory granulocytes in a dose-depe...ndent manner. Also, in these concentrations NCX4040 decreased production of nitric oxide in the cells culture supernatants. In conclusion, our results suggest that antiinflammatory properties of NO-aspirins are additionally potentiated by their proapoptotic effect on granulocytes, which could be a novel mechanism of their action.
Neutrofilni granulociti su deo imunog sistema, uključeni u odbranu organizma od mikroorganizama. Oni imaju najkraći životni vek među leukocitima, koji se može modulisati citokinima i farmakološkim agensima. Efekat novog derivata aspirina, NCX4040, na apoptozu inflamatornih granulocita do sada nije ispitivan. Zbog toga smo u ovom radu ispitivali efekat nitro-aspirina (NCX4040) na apoptozu inflamatornih neutrofilnih granulocita pacova. Inflamatorni granulociti su izolovani iz polivinilskih sunđera implantiranih potkožno pacovima Albino Oxford (AO) soja. Inflamatorne ćelije koje su izolovane 20 sati kasnije, najvećim delom (više od 95 %) su bili neutrofilni granulociti. Ove ćelije su kultivisane 24 sata sa NCX4040 u koncentracijama od 0.01 μM do 10 μM. Supernatanti kultura neutrofila su sakupljani, i korišćeni za merenje koncentracije NO. Ćelije su bojene propidijum jodidom i apoptoza je analizirana na protočnom citofluorimetru, kao i pomoću morfoloških kriterijuma. Ustanovili smo da u ko...ncentracijama od 0.25 μM do 10 μM ovaj nitro-aspirin indukuje, na dozno zavisan način, apoptozu neutrofilnih granulocita. Apoptoza indukovana nitro-aspirinom je bila u pozitivnoj korelaciji sa smanjenom produkcijom NO.
Ključne reči:
nitro-aspirin / neutrophil granulocytes / apoptosisIzvor:
Acta Veterinaria-Beograd, 2008, 58, 5-6, 449-457Izdavač:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
DOI: 10.2298/AVB0806449V
ISSN: 0567-8315
WoS: 000262356200004
Scopus: 2-s2.0-58249120736
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Vasilev, Saša AU - Vučević, Dragana AU - Gašić, Sonja AU - Majstorović, Ivana AU - Vasilijić, S. AU - Ćupić, Vitomir AU - Čolić, M. PY - 2008 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/557 AB - Apoptosis of neutrophil granulocytes is a critical event in the resolution of inflammation. Neutrophils have a short lifespan which can be modulated by aspirin. In this work we studied the effect of a nitroaspirin (NCX4040) on apoptosis of inflammatory granulocytes. This nitro-aspirin has been synthesized in attempt to reduce the side effects of aspirin in the gastrointestinal tract. Inflammatory granulocytes have been isolated from polyvinyl sponges implanted under the skin of Albino Oxford (AO) rats. Inflammatory cells that were isolated 20 hours later were about 95% neutrophil granulocytes. The cells were cultivated 24h with different concentrations of NCX4040 ranging from 0.01 μM to 10μM. After that period, apoptosis of neutrophils was performed by using morphological criteria, as well as by flow cytometry (after staining the cells with propidium iodide). We found that NCX4040 at concentrations from 0.25 to 10 μM induced the apoptosis of rat inflammatory granulocytes in a dose-dependent manner. Also, in these concentrations NCX4040 decreased production of nitric oxide in the cells culture supernatants. In conclusion, our results suggest that antiinflammatory properties of NO-aspirins are additionally potentiated by their proapoptotic effect on granulocytes, which could be a novel mechanism of their action. AB - Neutrofilni granulociti su deo imunog sistema, uključeni u odbranu organizma od mikroorganizama. Oni imaju najkraći životni vek među leukocitima, koji se može modulisati citokinima i farmakološkim agensima. Efekat novog derivata aspirina, NCX4040, na apoptozu inflamatornih granulocita do sada nije ispitivan. Zbog toga smo u ovom radu ispitivali efekat nitro-aspirina (NCX4040) na apoptozu inflamatornih neutrofilnih granulocita pacova. Inflamatorni granulociti su izolovani iz polivinilskih sunđera implantiranih potkožno pacovima Albino Oxford (AO) soja. Inflamatorne ćelije koje su izolovane 20 sati kasnije, najvećim delom (više od 95 %) su bili neutrofilni granulociti. Ove ćelije su kultivisane 24 sata sa NCX4040 u koncentracijama od 0.01 μM do 10 μM. Supernatanti kultura neutrofila su sakupljani, i korišćeni za merenje koncentracije NO. Ćelije su bojene propidijum jodidom i apoptoza je analizirana na protočnom citofluorimetru, kao i pomoću morfoloških kriterijuma. Ustanovili smo da u koncentracijama od 0.25 μM do 10 μM ovaj nitro-aspirin indukuje, na dozno zavisan način, apoptozu neutrofilnih granulocita. Apoptoza indukovana nitro-aspirinom je bila u pozitivnoj korelaciji sa smanjenom produkcijom NO. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - The effect of a new nitro-aspirin on apoptosis of neutrophil granulocytes T1 - Efekat novog nitro-aspirina na apoptozu neutrofilnih granulocita VL - 58 IS - 5-6 SP - 449 EP - 457 DO - 10.2298/AVB0806449V ER -
@article{ author = "Vasilev, Saša and Vučević, Dragana and Gašić, Sonja and Majstorović, Ivana and Vasilijić, S. and Ćupić, Vitomir and Čolić, M.", year = "2008", abstract = "Apoptosis of neutrophil granulocytes is a critical event in the resolution of inflammation. Neutrophils have a short lifespan which can be modulated by aspirin. In this work we studied the effect of a nitroaspirin (NCX4040) on apoptosis of inflammatory granulocytes. This nitro-aspirin has been synthesized in attempt to reduce the side effects of aspirin in the gastrointestinal tract. Inflammatory granulocytes have been isolated from polyvinyl sponges implanted under the skin of Albino Oxford (AO) rats. Inflammatory cells that were isolated 20 hours later were about 95% neutrophil granulocytes. The cells were cultivated 24h with different concentrations of NCX4040 ranging from 0.01 μM to 10μM. After that period, apoptosis of neutrophils was performed by using morphological criteria, as well as by flow cytometry (after staining the cells with propidium iodide). We found that NCX4040 at concentrations from 0.25 to 10 μM induced the apoptosis of rat inflammatory granulocytes in a dose-dependent manner. Also, in these concentrations NCX4040 decreased production of nitric oxide in the cells culture supernatants. In conclusion, our results suggest that antiinflammatory properties of NO-aspirins are additionally potentiated by their proapoptotic effect on granulocytes, which could be a novel mechanism of their action., Neutrofilni granulociti su deo imunog sistema, uključeni u odbranu organizma od mikroorganizama. Oni imaju najkraći životni vek među leukocitima, koji se može modulisati citokinima i farmakološkim agensima. Efekat novog derivata aspirina, NCX4040, na apoptozu inflamatornih granulocita do sada nije ispitivan. Zbog toga smo u ovom radu ispitivali efekat nitro-aspirina (NCX4040) na apoptozu inflamatornih neutrofilnih granulocita pacova. Inflamatorni granulociti su izolovani iz polivinilskih sunđera implantiranih potkožno pacovima Albino Oxford (AO) soja. Inflamatorne ćelije koje su izolovane 20 sati kasnije, najvećim delom (više od 95 %) su bili neutrofilni granulociti. Ove ćelije su kultivisane 24 sata sa NCX4040 u koncentracijama od 0.01 μM do 10 μM. Supernatanti kultura neutrofila su sakupljani, i korišćeni za merenje koncentracije NO. Ćelije su bojene propidijum jodidom i apoptoza je analizirana na protočnom citofluorimetru, kao i pomoću morfoloških kriterijuma. Ustanovili smo da u koncentracijama od 0.25 μM do 10 μM ovaj nitro-aspirin indukuje, na dozno zavisan način, apoptozu neutrofilnih granulocita. Apoptoza indukovana nitro-aspirinom je bila u pozitivnoj korelaciji sa smanjenom produkcijom NO.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "The effect of a new nitro-aspirin on apoptosis of neutrophil granulocytes, Efekat novog nitro-aspirina na apoptozu neutrofilnih granulocita", volume = "58", number = "5-6", pages = "449-457", doi = "10.2298/AVB0806449V" }
Vasilev, S., Vučević, D., Gašić, S., Majstorović, I., Vasilijić, S., Ćupić, V.,& Čolić, M.. (2008). The effect of a new nitro-aspirin on apoptosis of neutrophil granulocytes. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 58(5-6), 449-457. https://doi.org/10.2298/AVB0806449V
Vasilev S, Vučević D, Gašić S, Majstorović I, Vasilijić S, Ćupić V, Čolić M. The effect of a new nitro-aspirin on apoptosis of neutrophil granulocytes. in Acta Veterinaria-Beograd. 2008;58(5-6):449-457. doi:10.2298/AVB0806449V .
Vasilev, Saša, Vučević, Dragana, Gašić, Sonja, Majstorović, Ivana, Vasilijić, S., Ćupić, Vitomir, Čolić, M., "The effect of a new nitro-aspirin on apoptosis of neutrophil granulocytes" in Acta Veterinaria-Beograd, 58, no. 5-6 (2008):449-457, https://doi.org/10.2298/AVB0806449V . .