Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes
Samo za registrovane korisnike
2009
Autori
Bajić, VladanStanimirović, Zoran
Stevanović, Jevrosima
Spremo-Potparević, Biljana
Zivković, L.
Milicević, Z.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3′,5′ cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assay (CA) on human peripheral blood lymphocytes of healthy individuals and by bone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 μm) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p.) injection of 8-Cl-cAMP in doses of 10,80 and 150 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of respon...se when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP. These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.
Ključne reči:
8-Cl-cAMP / antitumor agents / micronuclei / premature centromere separationIzvor:
Journal of BUON, 2009, 14, 1, 71-77Izdavač:
- Balkan Union of Oncology (B.U.ON.)
Finansiranje / projekti:
- Evaluacija dejstva hormona i citostatika prmenom citogenetičkih analiza i Komet testa (RS-MESTD-MPN2006-2010-143018)
- Ekofiziološka i genetička istraživanja domaćih životinja i pčela u funkciji povećanja reproduktivnih svojstava i otpornosti na bolesti (RS-MESTD-MPN2006-2010-143022)
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Bajić, Vladan AU - Stanimirović, Zoran AU - Stevanović, Jevrosima AU - Spremo-Potparević, Biljana AU - Zivković, L. AU - Milicević, Z. PY - 2009 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/614 AB - Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3′,5′ cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assay (CA) on human peripheral blood lymphocytes of healthy individuals and by bone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 μm) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p.) injection of 8-Cl-cAMP in doses of 10,80 and 150 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP. These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents. PB - Balkan Union of Oncology (B.U.ON.) T2 - Journal of BUON T1 - Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes VL - 14 IS - 1 SP - 71 EP - 77 UR - https://hdl.handle.net/21.15107/rcub_veterinar_614 ER -
@article{ author = "Bajić, Vladan and Stanimirović, Zoran and Stevanović, Jevrosima and Spremo-Potparević, Biljana and Zivković, L. and Milicević, Z.", year = "2009", abstract = "Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3′,5′ cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assay (CA) on human peripheral blood lymphocytes of healthy individuals and by bone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 μm) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p.) injection of 8-Cl-cAMP in doses of 10,80 and 150 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP. These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.", publisher = "Balkan Union of Oncology (B.U.ON.)", journal = "Journal of BUON", title = "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes", volume = "14", number = "1", pages = "71-77", url = "https://hdl.handle.net/21.15107/rcub_veterinar_614" }
Bajić, V., Stanimirović, Z., Stevanović, J., Spremo-Potparević, B., Zivković, L.,& Milicević, Z.. (2009). Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON Balkan Union of Oncology (B.U.ON.)., 14(1), 71-77. https://hdl.handle.net/21.15107/rcub_veterinar_614
Bajić V, Stanimirović Z, Stevanović J, Spremo-Potparević B, Zivković L, Milicević Z. Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON. 2009;14(1):71-77. https://hdl.handle.net/21.15107/rcub_veterinar_614 .
Bajić, Vladan, Stanimirović, Zoran, Stevanović, Jevrosima, Spremo-Potparević, Biljana, Zivković, L., Milicević, Z., "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes" in Journal of BUON, 14, no. 1 (2009):71-77, https://hdl.handle.net/21.15107/rcub_veterinar_614 .