Cytogenetic changes in human lymphocytes induced by alphachlorhydrine
Citogenetičke promene limfocita čoveka indukovane alfahlorhidrinom
2010
Authors
Teodorović, RadislavaStanimirović, Zoran
Radenković-Damnjanović, Brana
Vučinić, Marijana
Janković, Ljiljana
Đorđević, Milutin
Mirilović, Milorad
Article (Published version)
Metadata
Show full item recordAbstract
This paper deals with the results of the investigation carried out with regard to genotoxic properties of Alphachlorhydrine administered at doses of 7.5, 15 and 30 mg/kg of cell culture-human lymphocytes, during an exposition time of three days, under in vitro conditions. The assessment of the genotoxic effects of the tested chemosterilants was performed on the basis of numerical and structural aberrations in three experimental groups, in addition to one control group for each dose. On the basis of the obtained results it can be conculded that Alphachlorhydrine, administered in the abovementioned doses, induced genotoxic effects on human lymphocyte cells. The number of numeric aberrations increased with a dose increase, and the statistical analysis showed significant differences (p lt 0.01) in relation to the control group, but not between the applied doses (p lt 0.05). Analysis of the obtained results demonstarted that a dose increase during the same exposition time, resulted in a sta...tistically significant increase (p lt 0.01) of structural aberrations in human lymphocyte cells; the highest level being achieved with the highest administered dose (30 mg/kg c.c.). As for Robertsonian translocations, there was a statistical significance with relation to the control group only in the case of the highest administered dose (30 mg/kg c.c.). Statistically significant differences (p lt 0.01) were recorded between the 30 mg/kg c.c. and 7.5 mg/kg c.c. doses. Also there were statistically significant differences between the levels of gaps and fragments in comparison to Robertsonian translocations (p lt 0.01); while the numbers of gaps and fragments were not significantly different.
U ovom radu su izvršena ispitivanja genotoksičnih svojstava Alfahlorhidrina u različitim dozama: 7.5, 15 i 30 mg/kg kulture ćelija - limfocita čoveka, tokom ekspozicije od 3 dana, u uslovima 'in vitro'. Procena genotoksičnih efekata, ispitivanih hemosterilanata, je vršena na osnovu numeričkih i strukturnih aberacija na tri eksperimentalne grupe sa po jednom kontrolnom za svaku dozu. Na osnovu rezultata naših ispitivanja može se zaključiti da Alfahlorhidrin u testiranim dozama dovodi do genotoksičnih efekata na ćelijama limfocita čoveka. Broj numeričkih aberacija se povećavao sa rastom doze i statističkom analizom su utvrđene signifikantne razlike (p lt 0,01) u odnosu na kontrolne grupe, ali ne i između primenjenih doza (p>0,05). U slučaju poliploidija nije utvrđena statistički značajna razlika u odnosu na kontrolnu grupu (p>0,05). Analizirajući dobijene rezultate utvrđeno je da sa povećanjem doze pri istoj ekspoziciji dolazi do statistički značajnog (p lt 0,01) povećanja broja struktur...nih aberacija na ćelijama limfocita čoveka, pri čemu najviši nivo je postignut kod najviše primenjene doze (30 mg/kg c.c.). Kada su u pitanju Robertsonove translokacije statistička značajnost u odnosu na kontrolnu grupu utvrđena je tek kod najviše primenjene doze (30 mg/kg c.c.). Statistički značajne razlike (p lt 0,01) su zabeležene između doza od 30 mg/kg c.c i 7,5 mg c.c. Takođe statistički značajne razlike između nivoa otvora i fragmenata mogu se uporediti sa Robertsonovim translokacijama (p lt 0,01), dok se broj otvora fragmenata neće značajno promeniti.
Keywords:
Alphachlorhydrine / cytogenetic / genotoxic effects / human / lymphocytesSource:
Acta Veterinaria-Beograd, 2010, 60, 5-6, 619-624Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
DOI: 10.2298/AVB1006619T
ISSN: 0567-8315
WoS: 000285772100017
Scopus: 2-s2.0-78651328533
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Fakultet veterinarske medicineTY - JOUR AU - Teodorović, Radislava AU - Stanimirović, Zoran AU - Radenković-Damnjanović, Brana AU - Vučinić, Marijana AU - Janković, Ljiljana AU - Đorđević, Milutin AU - Mirilović, Milorad PY - 2010 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/665 AB - This paper deals with the results of the investigation carried out with regard to genotoxic properties of Alphachlorhydrine administered at doses of 7.5, 15 and 30 mg/kg of cell culture-human lymphocytes, during an exposition time of three days, under in vitro conditions. The assessment of the genotoxic effects of the tested chemosterilants was performed on the basis of numerical and structural aberrations in three experimental groups, in addition to one control group for each dose. On the basis of the obtained results it can be conculded that Alphachlorhydrine, administered in the abovementioned doses, induced genotoxic effects on human lymphocyte cells. The number of numeric aberrations increased with a dose increase, and the statistical analysis showed significant differences (p lt 0.01) in relation to the control group, but not between the applied doses (p lt 0.05). Analysis of the obtained results demonstarted that a dose increase during the same exposition time, resulted in a statistically significant increase (p lt 0.01) of structural aberrations in human lymphocyte cells; the highest level being achieved with the highest administered dose (30 mg/kg c.c.). As for Robertsonian translocations, there was a statistical significance with relation to the control group only in the case of the highest administered dose (30 mg/kg c.c.). Statistically significant differences (p lt 0.01) were recorded between the 30 mg/kg c.c. and 7.5 mg/kg c.c. doses. Also there were statistically significant differences between the levels of gaps and fragments in comparison to Robertsonian translocations (p lt 0.01); while the numbers of gaps and fragments were not significantly different. AB - U ovom radu su izvršena ispitivanja genotoksičnih svojstava Alfahlorhidrina u različitim dozama: 7.5, 15 i 30 mg/kg kulture ćelija - limfocita čoveka, tokom ekspozicije od 3 dana, u uslovima 'in vitro'. Procena genotoksičnih efekata, ispitivanih hemosterilanata, je vršena na osnovu numeričkih i strukturnih aberacija na tri eksperimentalne grupe sa po jednom kontrolnom za svaku dozu. Na osnovu rezultata naših ispitivanja može se zaključiti da Alfahlorhidrin u testiranim dozama dovodi do genotoksičnih efekata na ćelijama limfocita čoveka. Broj numeričkih aberacija se povećavao sa rastom doze i statističkom analizom su utvrđene signifikantne razlike (p lt 0,01) u odnosu na kontrolne grupe, ali ne i između primenjenih doza (p>0,05). U slučaju poliploidija nije utvrđena statistički značajna razlika u odnosu na kontrolnu grupu (p>0,05). Analizirajući dobijene rezultate utvrđeno je da sa povećanjem doze pri istoj ekspoziciji dolazi do statistički značajnog (p lt 0,01) povećanja broja strukturnih aberacija na ćelijama limfocita čoveka, pri čemu najviši nivo je postignut kod najviše primenjene doze (30 mg/kg c.c.). Kada su u pitanju Robertsonove translokacije statistička značajnost u odnosu na kontrolnu grupu utvrđena je tek kod najviše primenjene doze (30 mg/kg c.c.). Statistički značajne razlike (p lt 0,01) su zabeležene između doza od 30 mg/kg c.c i 7,5 mg c.c. Takođe statistički značajne razlike između nivoa otvora i fragmenata mogu se uporediti sa Robertsonovim translokacijama (p lt 0,01), dok se broj otvora fragmenata neće značajno promeniti. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Cytogenetic changes in human lymphocytes induced by alphachlorhydrine T1 - Citogenetičke promene limfocita čoveka indukovane alfahlorhidrinom VL - 60 IS - 5-6 SP - 619 EP - 624 DO - 10.2298/AVB1006619T ER -
@article{ author = "Teodorović, Radislava and Stanimirović, Zoran and Radenković-Damnjanović, Brana and Vučinić, Marijana and Janković, Ljiljana and Đorđević, Milutin and Mirilović, Milorad", year = "2010", abstract = "This paper deals with the results of the investigation carried out with regard to genotoxic properties of Alphachlorhydrine administered at doses of 7.5, 15 and 30 mg/kg of cell culture-human lymphocytes, during an exposition time of three days, under in vitro conditions. The assessment of the genotoxic effects of the tested chemosterilants was performed on the basis of numerical and structural aberrations in three experimental groups, in addition to one control group for each dose. On the basis of the obtained results it can be conculded that Alphachlorhydrine, administered in the abovementioned doses, induced genotoxic effects on human lymphocyte cells. The number of numeric aberrations increased with a dose increase, and the statistical analysis showed significant differences (p lt 0.01) in relation to the control group, but not between the applied doses (p lt 0.05). Analysis of the obtained results demonstarted that a dose increase during the same exposition time, resulted in a statistically significant increase (p lt 0.01) of structural aberrations in human lymphocyte cells; the highest level being achieved with the highest administered dose (30 mg/kg c.c.). As for Robertsonian translocations, there was a statistical significance with relation to the control group only in the case of the highest administered dose (30 mg/kg c.c.). Statistically significant differences (p lt 0.01) were recorded between the 30 mg/kg c.c. and 7.5 mg/kg c.c. doses. Also there were statistically significant differences between the levels of gaps and fragments in comparison to Robertsonian translocations (p lt 0.01); while the numbers of gaps and fragments were not significantly different., U ovom radu su izvršena ispitivanja genotoksičnih svojstava Alfahlorhidrina u različitim dozama: 7.5, 15 i 30 mg/kg kulture ćelija - limfocita čoveka, tokom ekspozicije od 3 dana, u uslovima 'in vitro'. Procena genotoksičnih efekata, ispitivanih hemosterilanata, je vršena na osnovu numeričkih i strukturnih aberacija na tri eksperimentalne grupe sa po jednom kontrolnom za svaku dozu. Na osnovu rezultata naših ispitivanja može se zaključiti da Alfahlorhidrin u testiranim dozama dovodi do genotoksičnih efekata na ćelijama limfocita čoveka. Broj numeričkih aberacija se povećavao sa rastom doze i statističkom analizom su utvrđene signifikantne razlike (p lt 0,01) u odnosu na kontrolne grupe, ali ne i između primenjenih doza (p>0,05). U slučaju poliploidija nije utvrđena statistički značajna razlika u odnosu na kontrolnu grupu (p>0,05). Analizirajući dobijene rezultate utvrđeno je da sa povećanjem doze pri istoj ekspoziciji dolazi do statistički značajnog (p lt 0,01) povećanja broja strukturnih aberacija na ćelijama limfocita čoveka, pri čemu najviši nivo je postignut kod najviše primenjene doze (30 mg/kg c.c.). Kada su u pitanju Robertsonove translokacije statistička značajnost u odnosu na kontrolnu grupu utvrđena je tek kod najviše primenjene doze (30 mg/kg c.c.). Statistički značajne razlike (p lt 0,01) su zabeležene između doza od 30 mg/kg c.c i 7,5 mg c.c. Takođe statistički značajne razlike između nivoa otvora i fragmenata mogu se uporediti sa Robertsonovim translokacijama (p lt 0,01), dok se broj otvora fragmenata neće značajno promeniti.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Cytogenetic changes in human lymphocytes induced by alphachlorhydrine, Citogenetičke promene limfocita čoveka indukovane alfahlorhidrinom", volume = "60", number = "5-6", pages = "619-624", doi = "10.2298/AVB1006619T" }
Teodorović, R., Stanimirović, Z., Radenković-Damnjanović, B., Vučinić, M., Janković, L., Đorđević, M.,& Mirilović, M.. (2010). Cytogenetic changes in human lymphocytes induced by alphachlorhydrine. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(5-6), 619-624. https://doi.org/10.2298/AVB1006619T
Teodorović R, Stanimirović Z, Radenković-Damnjanović B, Vučinić M, Janković L, Đorđević M, Mirilović M. Cytogenetic changes in human lymphocytes induced by alphachlorhydrine. in Acta Veterinaria-Beograd. 2010;60(5-6):619-624. doi:10.2298/AVB1006619T .
Teodorović, Radislava, Stanimirović, Zoran, Radenković-Damnjanović, Brana, Vučinić, Marijana, Janković, Ljiljana, Đorđević, Milutin, Mirilović, Milorad, "Cytogenetic changes in human lymphocytes induced by alphachlorhydrine" in Acta Veterinaria-Beograd, 60, no. 5-6 (2010):619-624, https://doi.org/10.2298/AVB1006619T . .