Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle)
Abstract
Eleven microsatellite loci (TGLA227.BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA023, ETH3, ETH225, BM1824) were evaluated for their use in paternity testing in the Yugoslav Pied cattle (YU Simmental cattle) population in Serbia. A total of 40 animals were tested. At the 11 tested loci, a total of 91 alleles were detected. The mean number of alleles per locus was 8.273. Polymorphism information content (PIC) values ranged from 0.58 to 0.88 with the mean value of 0.72. The most informative loci were: TGLAS3 (14 alleles, PIC = 0.88), TGLA227(11 alleles, PIC = 0.82), INRA023 (11 alleles, PIC = 0.86), BM2113 (9 alleles, PIC = 0.80). Combined power of discrimination (CPD) for the 11 microsatellite loci was 0.999. The results of the present study confirm that the analysed set of 11 microsatellite markers recommended by ISAG is suitable for paternity testing in Yugoslav Pied cattle in Serbia.
Keywords:
molecular markers / informativeness / bovines / pedigree / SerbiaSource:
Czech Journal of Animal Science, 2010, 55, 6, 221-226Publisher:
- Czech Academy Agricultural Sciences, Prague
Funding / projects:
- Ecophysiological and genetic investigations of domestic animals and bees for the purpose of increasing reproductive traits and disease resistance (RS-MESTD-MPN2006-2010-143022)
- Ministry of Education and Science of Republic of Serbia [20011]
DOI: 10.17221/183/2009-CJAS
ISSN: 1212-1819
WoS: 000279340300001
Scopus: 2-s2.0-77954149948
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Stevanović, Jevrosima AU - Stanimirović, Zoran AU - Dimitrijević, Vladimir AU - Maletić, Milan PY - 2010 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/717 AB - Eleven microsatellite loci (TGLA227.BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA023, ETH3, ETH225, BM1824) were evaluated for their use in paternity testing in the Yugoslav Pied cattle (YU Simmental cattle) population in Serbia. A total of 40 animals were tested. At the 11 tested loci, a total of 91 alleles were detected. The mean number of alleles per locus was 8.273. Polymorphism information content (PIC) values ranged from 0.58 to 0.88 with the mean value of 0.72. The most informative loci were: TGLAS3 (14 alleles, PIC = 0.88), TGLA227(11 alleles, PIC = 0.82), INRA023 (11 alleles, PIC = 0.86), BM2113 (9 alleles, PIC = 0.80). Combined power of discrimination (CPD) for the 11 microsatellite loci was 0.999. The results of the present study confirm that the analysed set of 11 microsatellite markers recommended by ISAG is suitable for paternity testing in Yugoslav Pied cattle in Serbia. PB - Czech Academy Agricultural Sciences, Prague T2 - Czech Journal of Animal Science T1 - Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle) VL - 55 IS - 6 SP - 221 EP - 226 DO - 10.17221/183/2009-CJAS ER -
@article{ author = "Stevanović, Jevrosima and Stanimirović, Zoran and Dimitrijević, Vladimir and Maletić, Milan", year = "2010", abstract = "Eleven microsatellite loci (TGLA227.BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA023, ETH3, ETH225, BM1824) were evaluated for their use in paternity testing in the Yugoslav Pied cattle (YU Simmental cattle) population in Serbia. A total of 40 animals were tested. At the 11 tested loci, a total of 91 alleles were detected. The mean number of alleles per locus was 8.273. Polymorphism information content (PIC) values ranged from 0.58 to 0.88 with the mean value of 0.72. The most informative loci were: TGLAS3 (14 alleles, PIC = 0.88), TGLA227(11 alleles, PIC = 0.82), INRA023 (11 alleles, PIC = 0.86), BM2113 (9 alleles, PIC = 0.80). Combined power of discrimination (CPD) for the 11 microsatellite loci was 0.999. The results of the present study confirm that the analysed set of 11 microsatellite markers recommended by ISAG is suitable for paternity testing in Yugoslav Pied cattle in Serbia.", publisher = "Czech Academy Agricultural Sciences, Prague", journal = "Czech Journal of Animal Science", title = "Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle)", volume = "55", number = "6", pages = "221-226", doi = "10.17221/183/2009-CJAS" }
Stevanović, J., Stanimirović, Z., Dimitrijević, V.,& Maletić, M.. (2010). Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle). in Czech Journal of Animal Science Czech Academy Agricultural Sciences, Prague., 55(6), 221-226. https://doi.org/10.17221/183/2009-CJAS
Stevanović J, Stanimirović Z, Dimitrijević V, Maletić M. Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle). in Czech Journal of Animal Science. 2010;55(6):221-226. doi:10.17221/183/2009-CJAS .
Stevanović, Jevrosima, Stanimirović, Zoran, Dimitrijević, Vladimir, Maletić, Milan, "Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle)" in Czech Journal of Animal Science, 55, no. 6 (2010):221-226, https://doi.org/10.17221/183/2009-CJAS . .