The assessment of tolerability of prolonged oral eugenol administration in rats
Ispitivanje podnošljivosti produžene peroralne primene eugenola kod pacova
Abstract
The potential toxicity and general tolerability of eugenol following two-week or four-week continuous p.o. administration to rats has been investigated. An experiment was performed on 72 male rats of the Wistar strain. Four groups of rats were treated with different doses of eugenol (10 mg/kg bm/day, 50 mg/kg, 200 mg/kg and 400 mg/kg bm/day), the fifth group was administered vehicle (0.5 % methylcellulose, propylene glycol and water), and the sixth group comprised absolutely untreated controls. The corresponding doses of eugenol and vehicle were applied using a gastric probe in a volume of 1 ml/100 g body mass. The general tolerability of eugenol was evaluated on the basis of the daily intake of water and food, body mass, general health condition, behaviour, and lethality in the course of the experiment. In the investigated doses, eugenol applied p.o. in the course of two or four weeks does not influence significantly the intake of food, water, or body mass of rats. The dose of 400 mg/...kg/day produced undesired reactions (agitation and hyperesthesia) that were first observed on day 21 and lasted until the end of the experiment. Low subacute toxicity of eugenol was established following p.o. administration to rats. Eugenol in doses of 200 and 400 mg/kg tm/day has a low toxic potential and is safe for administration to this animal species.
Ispitivana je potencijalna toksičnost, odnosno opšta podnošljivost eugenola posle dvonedeljne i četvoronedeljne kontinuirane p.o. primene. Ogled je izveden na 72 mužjaka pacova soja vistar. Četiri grupe pacova tretirane su različitim dozama eugenola (10 mg/kg tm/dan, 50 mg/kg, 200 mg/kg i 400 mg/kg tm/dan), peta grupa dobijala je vehikulum (0,5 % metil-celuloza, propilen-glikol i voda), a šesta je bila kontrolna grupa. Odgovarajuće doze eugenola i vehikuluma aplikovane su gastričnom sondom u volumenu od 1 ml/100 g telesne mase. Opšta podnošljivost eugenola procenjivana je na osnovu dnevnog unosa vode i hrane, telesne mase, opšteg zdravstvenog stanja, ponaš anja i letaliteta tokom ogleda. U ispitivanim dozama eugenol primenjivan p.o. tokom dve ili četiri nedelje ne utiče značajno na unos hrane, vode i telesnu masu pacova. Doza od 400 mg/kg/dan dovela je do neželjenih reakcija (uznemirenost i hiperestezija) koje su prvi put zapažene 21. dana i trajale su do kraja ogleda. Ustanovljena je ...niska subakutna toksičnost eugenola posle p.o. primene kod pacova. Doze eugenola od 200 i 400 mg/kg tm/dan imaju nizak toksični potencijal i bezbedne su za primenu kod ove vrste životinja.
Keywords:
eugenol / toxicity / rat / food and water intake / eugenol / toksičnost / pacov / unos hrane i vodeSource:
Veterinarski Glasnik, 2012, 66, 3-4, 185-197Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
- Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Jezdimirović, Milanka AU - Aleksić, Nevenka AU - Trailović, Saša AU - Ivanović, Saša AU - Jezdimirović, Nemanja PY - 2012 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/862 AB - The potential toxicity and general tolerability of eugenol following two-week or four-week continuous p.o. administration to rats has been investigated. An experiment was performed on 72 male rats of the Wistar strain. Four groups of rats were treated with different doses of eugenol (10 mg/kg bm/day, 50 mg/kg, 200 mg/kg and 400 mg/kg bm/day), the fifth group was administered vehicle (0.5 % methylcellulose, propylene glycol and water), and the sixth group comprised absolutely untreated controls. The corresponding doses of eugenol and vehicle were applied using a gastric probe in a volume of 1 ml/100 g body mass. The general tolerability of eugenol was evaluated on the basis of the daily intake of water and food, body mass, general health condition, behaviour, and lethality in the course of the experiment. In the investigated doses, eugenol applied p.o. in the course of two or four weeks does not influence significantly the intake of food, water, or body mass of rats. The dose of 400 mg/kg/day produced undesired reactions (agitation and hyperesthesia) that were first observed on day 21 and lasted until the end of the experiment. Low subacute toxicity of eugenol was established following p.o. administration to rats. Eugenol in doses of 200 and 400 mg/kg tm/day has a low toxic potential and is safe for administration to this animal species. AB - Ispitivana je potencijalna toksičnost, odnosno opšta podnošljivost eugenola posle dvonedeljne i četvoronedeljne kontinuirane p.o. primene. Ogled je izveden na 72 mužjaka pacova soja vistar. Četiri grupe pacova tretirane su različitim dozama eugenola (10 mg/kg tm/dan, 50 mg/kg, 200 mg/kg i 400 mg/kg tm/dan), peta grupa dobijala je vehikulum (0,5 % metil-celuloza, propilen-glikol i voda), a šesta je bila kontrolna grupa. Odgovarajuće doze eugenola i vehikuluma aplikovane su gastričnom sondom u volumenu od 1 ml/100 g telesne mase. Opšta podnošljivost eugenola procenjivana je na osnovu dnevnog unosa vode i hrane, telesne mase, opšteg zdravstvenog stanja, ponaš anja i letaliteta tokom ogleda. U ispitivanim dozama eugenol primenjivan p.o. tokom dve ili četiri nedelje ne utiče značajno na unos hrane, vode i telesnu masu pacova. Doza od 400 mg/kg/dan dovela je do neželjenih reakcija (uznemirenost i hiperestezija) koje su prvi put zapažene 21. dana i trajale su do kraja ogleda. Ustanovljena je niska subakutna toksičnost eugenola posle p.o. primene kod pacova. Doze eugenola od 200 i 400 mg/kg tm/dan imaju nizak toksični potencijal i bezbedne su za primenu kod ove vrste životinja. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Veterinarski Glasnik T1 - The assessment of tolerability of prolonged oral eugenol administration in rats T1 - Ispitivanje podnošljivosti produžene peroralne primene eugenola kod pacova VL - 66 IS - 3-4 SP - 185 EP - 197 DO - 10.2298/VETGL1204185J ER -
@article{ author = "Jezdimirović, Milanka and Aleksić, Nevenka and Trailović, Saša and Ivanović, Saša and Jezdimirović, Nemanja", year = "2012", abstract = "The potential toxicity and general tolerability of eugenol following two-week or four-week continuous p.o. administration to rats has been investigated. An experiment was performed on 72 male rats of the Wistar strain. Four groups of rats were treated with different doses of eugenol (10 mg/kg bm/day, 50 mg/kg, 200 mg/kg and 400 mg/kg bm/day), the fifth group was administered vehicle (0.5 % methylcellulose, propylene glycol and water), and the sixth group comprised absolutely untreated controls. The corresponding doses of eugenol and vehicle were applied using a gastric probe in a volume of 1 ml/100 g body mass. The general tolerability of eugenol was evaluated on the basis of the daily intake of water and food, body mass, general health condition, behaviour, and lethality in the course of the experiment. In the investigated doses, eugenol applied p.o. in the course of two or four weeks does not influence significantly the intake of food, water, or body mass of rats. The dose of 400 mg/kg/day produced undesired reactions (agitation and hyperesthesia) that were first observed on day 21 and lasted until the end of the experiment. Low subacute toxicity of eugenol was established following p.o. administration to rats. Eugenol in doses of 200 and 400 mg/kg tm/day has a low toxic potential and is safe for administration to this animal species., Ispitivana je potencijalna toksičnost, odnosno opšta podnošljivost eugenola posle dvonedeljne i četvoronedeljne kontinuirane p.o. primene. Ogled je izveden na 72 mužjaka pacova soja vistar. Četiri grupe pacova tretirane su različitim dozama eugenola (10 mg/kg tm/dan, 50 mg/kg, 200 mg/kg i 400 mg/kg tm/dan), peta grupa dobijala je vehikulum (0,5 % metil-celuloza, propilen-glikol i voda), a šesta je bila kontrolna grupa. Odgovarajuće doze eugenola i vehikuluma aplikovane su gastričnom sondom u volumenu od 1 ml/100 g telesne mase. Opšta podnošljivost eugenola procenjivana je na osnovu dnevnog unosa vode i hrane, telesne mase, opšteg zdravstvenog stanja, ponaš anja i letaliteta tokom ogleda. U ispitivanim dozama eugenol primenjivan p.o. tokom dve ili četiri nedelje ne utiče značajno na unos hrane, vode i telesnu masu pacova. Doza od 400 mg/kg/dan dovela je do neželjenih reakcija (uznemirenost i hiperestezija) koje su prvi put zapažene 21. dana i trajale su do kraja ogleda. Ustanovljena je niska subakutna toksičnost eugenola posle p.o. primene kod pacova. Doze eugenola od 200 i 400 mg/kg tm/dan imaju nizak toksični potencijal i bezbedne su za primenu kod ove vrste životinja.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Veterinarski Glasnik", title = "The assessment of tolerability of prolonged oral eugenol administration in rats, Ispitivanje podnošljivosti produžene peroralne primene eugenola kod pacova", volume = "66", number = "3-4", pages = "185-197", doi = "10.2298/VETGL1204185J" }
Jezdimirović, M., Aleksić, N., Trailović, S., Ivanović, S.,& Jezdimirović, N.. (2012). The assessment of tolerability of prolonged oral eugenol administration in rats. in Veterinarski Glasnik Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3-4), 185-197. https://doi.org/10.2298/VETGL1204185J
Jezdimirović M, Aleksić N, Trailović S, Ivanović S, Jezdimirović N. The assessment of tolerability of prolonged oral eugenol administration in rats. in Veterinarski Glasnik. 2012;66(3-4):185-197. doi:10.2298/VETGL1204185J .
Jezdimirović, Milanka, Aleksić, Nevenka, Trailović, Saša, Ivanović, Saša, Jezdimirović, Nemanja, "The assessment of tolerability of prolonged oral eugenol administration in rats" in Veterinarski Glasnik, 66, no. 3-4 (2012):185-197, https://doi.org/10.2298/VETGL1204185J . .