Application of standard and molecular methods for the diagnosis of Newcastle disease
2012
Authors
Vidanović, DejanŠekler, Milanko
Polaček, Vladimir
Vasković, Nikola
Ašanin, Ružica
Milić, Nenad
Nišavić, Jakov
Article (Published version)
Metadata
Show full item recordAbstract
Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007. Newcastle disease virus strains were isolated from four samples. The identification of isolated strains was done by using the hemagglutination and hemagglutination-inhibition tests. The nucleic acid of the ND virus was identified in all the four samples It was confirmed that all the isolated strains were velogenic strains. Analysis of the nucleotide sequences of the gene encoding the F cleavage site of the fusion F protein showed the presence of motifs 112RRQKRFIG119, characteristic for the velogenic strains of the ND virus. Phylogenetic analysis of the F gene sequences revealed that all isolated strains of the virus belong to class II and genotype VIId.
Keywords:
Newcastle disease virus / RT-PCR / Real Time RT-PCRSource:
Archives of Biological Sciences, 2012, 64, 4, 1433-1437Publisher:
- Srpsko biološko društvo, Beograd, i dr.
Funding / projects:
- Development and application of molecular methods based on polymerase chain reaction (PCR) in rapid and direct identification of Newcastle disease virus strains and examination of immunogenicity of subunit vaccine prepared from their antigens (RS-MESTD-Technological Development (TD or TR)-31008)
DOI: 10.2298/ABS1204433V
ISSN: 0354-4664
WoS: 000312046200025
Scopus: 2-s2.0-84867795676
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Vidanović, Dejan AU - Šekler, Milanko AU - Polaček, Vladimir AU - Vasković, Nikola AU - Ašanin, Ružica AU - Milić, Nenad AU - Nišavić, Jakov PY - 2012 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/945 AB - Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007. Newcastle disease virus strains were isolated from four samples. The identification of isolated strains was done by using the hemagglutination and hemagglutination-inhibition tests. The nucleic acid of the ND virus was identified in all the four samples It was confirmed that all the isolated strains were velogenic strains. Analysis of the nucleotide sequences of the gene encoding the F cleavage site of the fusion F protein showed the presence of motifs 112RRQKRFIG119, characteristic for the velogenic strains of the ND virus. Phylogenetic analysis of the F gene sequences revealed that all isolated strains of the virus belong to class II and genotype VIId. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Application of standard and molecular methods for the diagnosis of Newcastle disease VL - 64 IS - 4 SP - 1433 EP - 1437 DO - 10.2298/ABS1204433V ER -
@article{ author = "Vidanović, Dejan and Šekler, Milanko and Polaček, Vladimir and Vasković, Nikola and Ašanin, Ružica and Milić, Nenad and Nišavić, Jakov", year = "2012", abstract = "Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007. Newcastle disease virus strains were isolated from four samples. The identification of isolated strains was done by using the hemagglutination and hemagglutination-inhibition tests. The nucleic acid of the ND virus was identified in all the four samples It was confirmed that all the isolated strains were velogenic strains. Analysis of the nucleotide sequences of the gene encoding the F cleavage site of the fusion F protein showed the presence of motifs 112RRQKRFIG119, characteristic for the velogenic strains of the ND virus. Phylogenetic analysis of the F gene sequences revealed that all isolated strains of the virus belong to class II and genotype VIId.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Application of standard and molecular methods for the diagnosis of Newcastle disease", volume = "64", number = "4", pages = "1433-1437", doi = "10.2298/ABS1204433V" }
Vidanović, D., Šekler, M., Polaček, V., Vasković, N., Ašanin, R., Milić, N.,& Nišavić, J.. (2012). Application of standard and molecular methods for the diagnosis of Newcastle disease. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 64(4), 1433-1437. https://doi.org/10.2298/ABS1204433V
Vidanović D, Šekler M, Polaček V, Vasković N, Ašanin R, Milić N, Nišavić J. Application of standard and molecular methods for the diagnosis of Newcastle disease. in Archives of Biological Sciences. 2012;64(4):1433-1437. doi:10.2298/ABS1204433V .
Vidanović, Dejan, Šekler, Milanko, Polaček, Vladimir, Vasković, Nikola, Ašanin, Ružica, Milić, Nenad, Nišavić, Jakov, "Application of standard and molecular methods for the diagnosis of Newcastle disease" in Archives of Biological Sciences, 64, no. 4 (2012):1433-1437, https://doi.org/10.2298/ABS1204433V . .