TY  - JOUR
AU  - Šarić, Ljubiša
AU  - Premović, Tamara
AU  - Šarić, Bojana
AU  - Čabarkapa, Ivana
AU  - Todorić, Olja
AU  - Miljanić, Jelena
AU  - Lazarević, Jasmina
AU  - Karabasil, Nedjeljko
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2556
AB  - The aim of this study was to examine the microbiological quality of raw donkey milk of an indigenous Serbian breed as well as the changes in the microbial populations during storage at 4 °C. In addition, antibacterial activity of donkey milk against E. coli, L. monocytogenes and S. aureus at 15 °C as well as the content of the two main antibacterial proteins lysozyme and lactoferrin were investigated. Microbiological examination of 137 individual milk samples collected over a period of 21 months showed good microbiological quality since foodborne pathogens such as Salmonella spp. and L. monocytogenes were not detected in any of the analyzed samples, while the number of E. coli, Enterobacteriaceae, total coliform bacteria, sulfite-reducing Clostridia and aerobic sporogenic bacteria was below the limit of quantification (<1 cfu mL−1). During the six-days storage at 4 °C, total bacterial counts and the counts of lactic acid bacteria remained at the initial level while pathogenic bacteria were not detected. The strongest antibacterial activity of the tested milk was observed against E. coli, while S. aureus was the least sensitive to milk antibacterial compounds. Although further research is needed to fully elucidate the antibacterial mechanism and synergistic activity of different compounds in donkey milk, the high content lysozyme (2.63 ± 0.03 g L−1) and lactoferrin (15.48 mg L−1) observed in tested milk could contribute to its strong antibacterial activity and extension of the storage period during which it can be safely consumed.
PB  - MDPI
T2  - Animals
T1  - Microbiological Quality of Raw Donkey Milk from Serbia and Its Antibacterial Properties at Pre-Cooling Temperature
VL  - 13
IS  - 3
SP  - 327
DO  - 10.3390/ani13030327
ER  - 

@article{
author = "Šarić, Ljubiša and Premović, Tamara and Šarić, Bojana and Čabarkapa, Ivana and Todorić, Olja and Miljanić, Jelena and Lazarević, Jasmina and Karabasil, Nedjeljko",
year = "2023",
abstract = "The aim of this study was to examine the microbiological quality of raw donkey milk of an indigenous Serbian breed as well as the changes in the microbial populations during storage at 4 °C. In addition, antibacterial activity of donkey milk against E. coli, L. monocytogenes and S. aureus at 15 °C as well as the content of the two main antibacterial proteins lysozyme and lactoferrin were investigated. Microbiological examination of 137 individual milk samples collected over a period of 21 months showed good microbiological quality since foodborne pathogens such as Salmonella spp. and L. monocytogenes were not detected in any of the analyzed samples, while the number of E. coli, Enterobacteriaceae, total coliform bacteria, sulfite-reducing Clostridia and aerobic sporogenic bacteria was below the limit of quantification (<1 cfu mL−1). During the six-days storage at 4 °C, total bacterial counts and the counts of lactic acid bacteria remained at the initial level while pathogenic bacteria were not detected. The strongest antibacterial activity of the tested milk was observed against E. coli, while S. aureus was the least sensitive to milk antibacterial compounds. Although further research is needed to fully elucidate the antibacterial mechanism and synergistic activity of different compounds in donkey milk, the high content lysozyme (2.63 ± 0.03 g L−1) and lactoferrin (15.48 mg L−1) observed in tested milk could contribute to its strong antibacterial activity and extension of the storage period during which it can be safely consumed.",
publisher = "MDPI",
journal = "Animals",
title = "Microbiological Quality of Raw Donkey Milk from Serbia and Its Antibacterial Properties at Pre-Cooling Temperature",
volume = "13",
number = "3",
pages = "327",
doi = "10.3390/ani13030327"
}

Šarić, L., Premović, T., Šarić, B., Čabarkapa, I., Todorić, O., Miljanić, J., Lazarević, J.,& Karabasil, N.. (2023). Microbiological Quality of Raw Donkey Milk from Serbia and Its Antibacterial Properties at Pre-Cooling Temperature. in Animals
MDPI., 13(3), 327.
https://doi.org/10.3390/ani13030327

Šarić L, Premović T, Šarić B, Čabarkapa I, Todorić O, Miljanić J, Lazarević J, Karabasil N. Microbiological Quality of Raw Donkey Milk from Serbia and Its Antibacterial Properties at Pre-Cooling Temperature. in Animals. 2023;13(3):327.
doi:10.3390/ani13030327 .

Šarić, Ljubiša, Premović, Tamara, Šarić, Bojana, Čabarkapa, Ivana, Todorić, Olja, Miljanić, Jelena, Lazarević, Jasmina, Karabasil, Nedjeljko, "Microbiological Quality of Raw Donkey Milk from Serbia and Its Antibacterial Properties at Pre-Cooling Temperature" in Animals, 13, no. 3 (2023):327,
https://doi.org/10.3390/ani13030327 . .

TY  - JOUR
AU  - Tomanić, Dragana
AU  - Božin, Biljana
AU  - Čabarkapa, Ivana
AU  - Kladar, Nebojša
AU  - Radinović, Miodrag
AU  - Maletić, Milan
AU  - Kovačević, Zorana
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2458
AB  - Mastitis is one of the most common and costly diseases affecting dairy cows worldwide. Since antibiotic resistance has become a global threat to both animal and human health, it is becoming more urgent to continuously search for new therapeutical alternatives for the control and treatment of bovine mastitis. Hence, our research aimed to test the therapeutic use of two essential oils (EOs) based on their chemical composition, antibacterial and antioxidant potential. The present study was conducted by collecting milk samples from the cows diagnosed with clinical or subclinical mastitis with the aim of isolating and identifying bacterial strains. The antioxidant potential of essential oils of Menthae piperitae (MP) and Melissa officinalis (MO) was evaluated in several in vitro assays. In the MP EO, a total of 38 compounds were identified, with menthol as the dominant compound, whereas in MO EO 51 compounds were identified. Furthermore, the values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) have been used to quantitatively measure the antibacterial activity of each essential oil. In accordance with which, MP EO samples exhibited a higher degree of antibacterial activity than MO EO. Thus, EOs have been shown to be promising alternatives to antibiotics because of their  availability,  biodegradability,  and lower risk of  side effects as compared with conventional, antimicrobial treatment. Nevertheless,  further  clinical  studies  are  needed  to  test  the  potential  role  of   EOs  in  treating mastitis in dairy cows.
PB  - Vaculty of Veterinary Medicine, University of Belgrade
T2  - Acta Veterinaria
T1  - Chemical composition, antioxidant and antibacterial activity of two different essential oils against mastitis associated pathogens
T1  - Hemijski sastav, antioksidativni potencijal i antibakterijska aktivnost dva različita etarska ulja protiv uzročnika mastitisa
VL  - 72
IS  - 1
SP  - 45
EP  - 58
DO  - 10.2478/acve-2022-0004
ER  - 

@article{
author = "Tomanić, Dragana and Božin, Biljana and Čabarkapa, Ivana and Kladar, Nebojša and Radinović, Miodrag and Maletić, Milan and Kovačević, Zorana",
year = "2022",
abstract = "Mastitis is one of the most common and costly diseases affecting dairy cows worldwide. Since antibiotic resistance has become a global threat to both animal and human health, it is becoming more urgent to continuously search for new therapeutical alternatives for the control and treatment of bovine mastitis. Hence, our research aimed to test the therapeutic use of two essential oils (EOs) based on their chemical composition, antibacterial and antioxidant potential. The present study was conducted by collecting milk samples from the cows diagnosed with clinical or subclinical mastitis with the aim of isolating and identifying bacterial strains. The antioxidant potential of essential oils of Menthae piperitae (MP) and Melissa officinalis (MO) was evaluated in several in vitro assays. In the MP EO, a total of 38 compounds were identified, with menthol as the dominant compound, whereas in MO EO 51 compounds were identified. Furthermore, the values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) have been used to quantitatively measure the antibacterial activity of each essential oil. In accordance with which, MP EO samples exhibited a higher degree of antibacterial activity than MO EO. Thus, EOs have been shown to be promising alternatives to antibiotics because of their  availability,  biodegradability,  and lower risk of  side effects as compared with conventional, antimicrobial treatment. Nevertheless,  further  clinical  studies  are  needed  to  test  the  potential  role  of   EOs  in  treating mastitis in dairy cows.",
publisher = "Vaculty of Veterinary Medicine, University of Belgrade",
journal = "Acta Veterinaria",
title = "Chemical composition, antioxidant and antibacterial activity of two different essential oils against mastitis associated pathogens, Hemijski sastav, antioksidativni potencijal i antibakterijska aktivnost dva različita etarska ulja protiv uzročnika mastitisa",
volume = "72",
number = "1",
pages = "45-58",
doi = "10.2478/acve-2022-0004"
}

Tomanić, D., Božin, B., Čabarkapa, I., Kladar, N., Radinović, M., Maletić, M.,& Kovačević, Z.. (2022). Chemical composition, antioxidant and antibacterial activity of two different essential oils against mastitis associated pathogens. in Acta Veterinaria
Vaculty of Veterinary Medicine, University of Belgrade., 72(1), 45-58.
https://doi.org/10.2478/acve-2022-0004

Tomanić D, Božin B, Čabarkapa I, Kladar N, Radinović M, Maletić M, Kovačević Z. Chemical composition, antioxidant and antibacterial activity of two different essential oils against mastitis associated pathogens. in Acta Veterinaria. 2022;72(1):45-58.
doi:10.2478/acve-2022-0004 .

Tomanić, Dragana, Božin, Biljana, Čabarkapa, Ivana, Kladar, Nebojša, Radinović, Miodrag, Maletić, Milan, Kovačević, Zorana, "Chemical composition, antioxidant and antibacterial activity of two different essential oils against mastitis associated pathogens" in Acta Veterinaria, 72, no. 1 (2022):45-58,
https://doi.org/10.2478/acve-2022-0004 . .

TY  - JOUR
AU  - Kovačević, Zorana
AU  - Kladar, Nebojša
AU  - Čabarkapa, Ivana
AU  - Radinović, Miodrag
AU  - Maletić, Milan
AU  - Erdeljan, Mihajlo
AU  - Božin, Biljana
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2390
AB  - Mastitis represents a heavy burden for the dairy sector worldwide with high economic and animal welfare impact. Antibiotic treatment is an important component of mastitis control programs. However, emergence and transfer of antimicrobial-resistant (AMR) bacteria is becoming a growing concern. Therefore, the development of novel agents is required for prevention and treatment of mastitis. Hence, our aim was to assess the antibacterial properties of two essential oils (EOs) obtained from oregano (Origanum vulgare L., Lamiaceae) and mountain savory (Satureja montana L., Lamiaceae) against mastitis-associated bacteria in Serbia. The chemical composition and antioxidant potential of these EOs were also evaluated. The present study was conducted on strains derived from aseptic milk samples collected from Holstein-Friesian cows with clinical or subclinical mastitis, during the morning milking. Clinical mastitis was assessed by clinical examination, while subclinical mastitis was confirmed using somatic cell count in the milk samples. The microdilution method was used to determine the antibacterial activity, while antioxidant potential of the EOs was evaluated in several in vitro assays. The values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were used to quantitatively measure the antibacterial activity of each EO. MIC/MBC ranged from 0.78/6.25 and 0.39/0.78 mg/mL for oregano and mountain savory, respectively. A total of 25 compounds were identified in the oregano EO, while 47 were identified in winter savory EO, among which aromatic oxygenated monoterpenes were the most abundant compounds. The tested EOs have shown promising antimicrobial activity and could be considered as one of the treatment approaches in mastitis-affected cows.
PB  - MDPI
T2  - Antibiotics
T1  - New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives
VL  - 10
IS  - 12
DO  - 10.3390/antibiotics10121460
ER  - 

@article{
author = "Kovačević, Zorana and Kladar, Nebojša and Čabarkapa, Ivana and Radinović, Miodrag and Maletić, Milan and Erdeljan, Mihajlo and Božin, Biljana",
year = "2021",
abstract = "Mastitis represents a heavy burden for the dairy sector worldwide with high economic and animal welfare impact. Antibiotic treatment is an important component of mastitis control programs. However, emergence and transfer of antimicrobial-resistant (AMR) bacteria is becoming a growing concern. Therefore, the development of novel agents is required for prevention and treatment of mastitis. Hence, our aim was to assess the antibacterial properties of two essential oils (EOs) obtained from oregano (Origanum vulgare L., Lamiaceae) and mountain savory (Satureja montana L., Lamiaceae) against mastitis-associated bacteria in Serbia. The chemical composition and antioxidant potential of these EOs were also evaluated. The present study was conducted on strains derived from aseptic milk samples collected from Holstein-Friesian cows with clinical or subclinical mastitis, during the morning milking. Clinical mastitis was assessed by clinical examination, while subclinical mastitis was confirmed using somatic cell count in the milk samples. The microdilution method was used to determine the antibacterial activity, while antioxidant potential of the EOs was evaluated in several in vitro assays. The values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were used to quantitatively measure the antibacterial activity of each EO. MIC/MBC ranged from 0.78/6.25 and 0.39/0.78 mg/mL for oregano and mountain savory, respectively. A total of 25 compounds were identified in the oregano EO, while 47 were identified in winter savory EO, among which aromatic oxygenated monoterpenes were the most abundant compounds. The tested EOs have shown promising antimicrobial activity and could be considered as one of the treatment approaches in mastitis-affected cows.",
publisher = "MDPI",
journal = "Antibiotics",
title = "New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives",
volume = "10",
number = "12",
doi = "10.3390/antibiotics10121460"
}

Kovačević, Z., Kladar, N., Čabarkapa, I., Radinović, M., Maletić, M., Erdeljan, M.,& Božin, B.. (2021). New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives. in Antibiotics
MDPI., 10(12).
https://doi.org/10.3390/antibiotics10121460

Kovačević Z, Kladar N, Čabarkapa I, Radinović M, Maletić M, Erdeljan M, Božin B. New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives. in Antibiotics. 2021;10(12).
doi:10.3390/antibiotics10121460 .

Kovačević, Zorana, Kladar, Nebojša, Čabarkapa, Ivana, Radinović, Miodrag, Maletić, Milan, Erdeljan, Mihajlo, Božin, Biljana, "New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives" in Antibiotics, 10, no. 12 (2021),
https://doi.org/10.3390/antibiotics10121460 . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Aleksić, Nevenka
AU  - Vidaković, Suzana
AU  - Ljubojević, Dragana
AU  - Čabarkapa, Ivana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1713
AB  - Commercial feed intended for dogs and cats is an almost unrecognised source of human infection with various serovars of Salmonella enterica. However, people may catch the infection both via direct contact with contaminated pet feed and by contact with pets, which usually shed Salmonella without signs of infection. A relatively new trend of feeding dogs and cats with raw feed is considered to be a special risk owing to the fact that it usually contains foodborne pathogens, such as Salmonella spp., Escherichia coli and Campylobacter spp. Nonetheless, the epidemiological data do not support this idea. In the current review relevant data on the significance of pet feed in the outbreak of human salmonellosis are discussed and the recommendations for the prevention of the infection originating from these sources are suggested.
AB  - Komercijalna hrana za pse i mačke je skoro nepoznat izvor infekcije ljudi bakterijama iz roda Salmonella. Ljudi se mogu inficirati direktnim kontaktom sa kontaminiranom hranom za kućne ljubimce ili kontaktom sa kućnim ljubimcima koji uobičajeno izlučuju salmonele bez kliničkih znakova infekcije. Iako se novi trend ishrane pasa i mačaka sirovom hranom sa tog aspekta razmatra kao poseban rizik (jer ova hrana uobičajeno sadrži hranom prenosive patogene kao što su Salmonella spp., Escherichia coli i Campylobacter spp.), epidemiološki podaci ne podržavaju ovakav stav. U ovom radu sumiramo relevatne podatke o značaju hrane za kućne ljubimce u pojavi salmoneloza ljudi, kao i preporuke za prevenciju pojave infekcija izazvanih salmonelama iz ovog izvora.
PB  - Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad
T2  - Food and Feed Research
T1  - Salmonella spp. in pet feed and risk it poses to humans
T1  - Salmonella spp. u hrani za kućne ljubimce i rizik koji predstavlja za zdravlje ljudi
VL  - 46
IS  - 1
SP  - 137
EP  - 145
DO  - 10.5937/FFR1901137M
ER  - 

@article{
author = "Milanov, Dubravka and Aleksić, Nevenka and Vidaković, Suzana and Ljubojević, Dragana and Čabarkapa, Ivana",
year = "2019",
abstract = "Commercial feed intended for dogs and cats is an almost unrecognised source of human infection with various serovars of Salmonella enterica. However, people may catch the infection both via direct contact with contaminated pet feed and by contact with pets, which usually shed Salmonella without signs of infection. A relatively new trend of feeding dogs and cats with raw feed is considered to be a special risk owing to the fact that it usually contains foodborne pathogens, such as Salmonella spp., Escherichia coli and Campylobacter spp. Nonetheless, the epidemiological data do not support this idea. In the current review relevant data on the significance of pet feed in the outbreak of human salmonellosis are discussed and the recommendations for the prevention of the infection originating from these sources are suggested., Komercijalna hrana za pse i mačke je skoro nepoznat izvor infekcije ljudi bakterijama iz roda Salmonella. Ljudi se mogu inficirati direktnim kontaktom sa kontaminiranom hranom za kućne ljubimce ili kontaktom sa kućnim ljubimcima koji uobičajeno izlučuju salmonele bez kliničkih znakova infekcije. Iako se novi trend ishrane pasa i mačaka sirovom hranom sa tog aspekta razmatra kao poseban rizik (jer ova hrana uobičajeno sadrži hranom prenosive patogene kao što su Salmonella spp., Escherichia coli i Campylobacter spp.), epidemiološki podaci ne podržavaju ovakav stav. U ovom radu sumiramo relevatne podatke o značaju hrane za kućne ljubimce u pojavi salmoneloza ljudi, kao i preporuke za prevenciju pojave infekcija izazvanih salmonelama iz ovog izvora.",
publisher = "Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad",
journal = "Food and Feed Research",
title = "Salmonella spp. in pet feed and risk it poses to humans, Salmonella spp. u hrani za kućne ljubimce i rizik koji predstavlja za zdravlje ljudi",
volume = "46",
number = "1",
pages = "137-145",
doi = "10.5937/FFR1901137M"
}

Milanov, D., Aleksić, N., Vidaković, S., Ljubojević, D.,& Čabarkapa, I.. (2019). Salmonella spp. in pet feed and risk it poses to humans. in Food and Feed Research
Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad., 46(1), 137-145.
https://doi.org/10.5937/FFR1901137M

Milanov D, Aleksić N, Vidaković S, Ljubojević D, Čabarkapa I. Salmonella spp. in pet feed and risk it poses to humans. in Food and Feed Research. 2019;46(1):137-145.
doi:10.5937/FFR1901137M .

Milanov, Dubravka, Aleksić, Nevenka, Vidaković, Suzana, Ljubojević, Dragana, Čabarkapa, Ivana, "Salmonella spp. in pet feed and risk it poses to humans" in Food and Feed Research, 46, no. 1 (2019):137-145,
https://doi.org/10.5937/FFR1901137M . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Petrović, Tamaš
AU  - Todorović, Dalibor
AU  - Aleksić, Nevenka
AU  - Čabarkapa, Ivana
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1642
AB  - Clostridium perfringens is a Gram-positive, endospore-forming, anaerobic rod, ubiquitous in nature. C. perfringens strains can produce about 17 toxins. Many of them can lead to miscellaneous diseases, among which the enteric ailment may be the most common and is of utmost importance. In the present work 34 strains of C. perfringens isolated from feed and one from a cow suspected to have died of clostridial infection were subjected to molecular analysis. In order to detect the genotypes, the following genes coding for toxins were targetted: cpa, cpb, cpb2, cpe, etx and iap. The multiplex PCR assay revealed that all C. perfringens isolates from animal feed were of type A and b2-toxinogenic type A strains, possessing only the cpa (n=21), or both the cpa and the cpb2 genes (n=13). The importance of C. perfringens toxins α and β-2 in the pathogenesis of enterotoxemia is discussed and the regulation on the detection of this bacteria in animal feed questioned. The use of PCR in practise could enable the toxin-genotyping of C. perfringens isolates and, thus, provide a real basis for the establishment of maximum acceptable limits of this bacteria in feed.
AB  - Clostridium perfringens je Gram-pozitivna, anaerobna, sporulišuća, štapićasta bakterija, ubikvitarno rasprostranjena u prirodi. Kod različitih sojeva C. Perfringens, do danas, identifikovano je oko 17 vrsta toksina. C. perfringens je uzročni agens različitih oboljenja (sindroma), ali su crevne infekcije/intoksikacije najčešće i od najvećeg značaja za zdravlje farmski gajenih životinja. U ovom radu prikazujemo rezultate ispitivanja sojeva C. perfringens poreklom iz hrane za životinje (n=34) i jednog izolata iz organa krave uginule sa znacima enterotoksemije na prisustvo gena: cpa, cpb, cpb2, cpe, etx i iap primenom multipleks PCR tehnike. Svi sojevi C. perfringens izolovani iz hrane za životinje, identifikovani su kao tip A koji poseduje samo cpa gen (n=21) ili tip A koji produkuje b2-toksin, odnosno ima cpa i cpb2 gene (n=13). U radu diskutujemo o ulozi alfa (α) i beta-2 (β2) toksina u patogenezi enterotoksemija domaćih životinja, kao i aktuelnom zakonskom propisu po kojem ova vrsta bakterije ne sme biti prisutna u hrani za životinje. Primena PCR tehnike u svakodnevnoj praksi omogućila bi toksin-genotipizaciju sojeva C. perfringens, a time i realne osnove za uspostavljanje graničnih dozvoljenih vrednosti za ovu vrstu bakterije u hrani za životinje.
PB  - Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad
T2  - Food and Feed Research
T1  - Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals
T1  - Toksin genotipizacija sojeva Clostridium perfringens izolovanih iz hrane za životinje i njihov značaj u etiologiji enterotoksemija domaćih životinja
VL  - 45
IS  - 1
SP  - 67
EP  - 76
DO  - 10.5937/FFR1801067M
ER  - 

@article{
author = "Milanov, Dubravka and Petrović, Tamaš and Todorović, Dalibor and Aleksić, Nevenka and Čabarkapa, Ivana",
year = "2018",
abstract = "Clostridium perfringens is a Gram-positive, endospore-forming, anaerobic rod, ubiquitous in nature. C. perfringens strains can produce about 17 toxins. Many of them can lead to miscellaneous diseases, among which the enteric ailment may be the most common and is of utmost importance. In the present work 34 strains of C. perfringens isolated from feed and one from a cow suspected to have died of clostridial infection were subjected to molecular analysis. In order to detect the genotypes, the following genes coding for toxins were targetted: cpa, cpb, cpb2, cpe, etx and iap. The multiplex PCR assay revealed that all C. perfringens isolates from animal feed were of type A and b2-toxinogenic type A strains, possessing only the cpa (n=21), or both the cpa and the cpb2 genes (n=13). The importance of C. perfringens toxins α and β-2 in the pathogenesis of enterotoxemia is discussed and the regulation on the detection of this bacteria in animal feed questioned. The use of PCR in practise could enable the toxin-genotyping of C. perfringens isolates and, thus, provide a real basis for the establishment of maximum acceptable limits of this bacteria in feed., Clostridium perfringens je Gram-pozitivna, anaerobna, sporulišuća, štapićasta bakterija, ubikvitarno rasprostranjena u prirodi. Kod različitih sojeva C. Perfringens, do danas, identifikovano je oko 17 vrsta toksina. C. perfringens je uzročni agens različitih oboljenja (sindroma), ali su crevne infekcije/intoksikacije najčešće i od najvećeg značaja za zdravlje farmski gajenih životinja. U ovom radu prikazujemo rezultate ispitivanja sojeva C. perfringens poreklom iz hrane za životinje (n=34) i jednog izolata iz organa krave uginule sa znacima enterotoksemije na prisustvo gena: cpa, cpb, cpb2, cpe, etx i iap primenom multipleks PCR tehnike. Svi sojevi C. perfringens izolovani iz hrane za životinje, identifikovani su kao tip A koji poseduje samo cpa gen (n=21) ili tip A koji produkuje b2-toksin, odnosno ima cpa i cpb2 gene (n=13). U radu diskutujemo o ulozi alfa (α) i beta-2 (β2) toksina u patogenezi enterotoksemija domaćih životinja, kao i aktuelnom zakonskom propisu po kojem ova vrsta bakterije ne sme biti prisutna u hrani za životinje. Primena PCR tehnike u svakodnevnoj praksi omogućila bi toksin-genotipizaciju sojeva C. perfringens, a time i realne osnove za uspostavljanje graničnih dozvoljenih vrednosti za ovu vrstu bakterije u hrani za životinje.",
publisher = "Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad",
journal = "Food and Feed Research",
title = "Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals, Toksin genotipizacija sojeva Clostridium perfringens izolovanih iz hrane za životinje i njihov značaj u etiologiji enterotoksemija domaćih životinja",
volume = "45",
number = "1",
pages = "67-76",
doi = "10.5937/FFR1801067M"
}

Milanov, D., Petrović, T., Todorović, D., Aleksić, N.,& Čabarkapa, I.. (2018). Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals. in Food and Feed Research
Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad., 45(1), 67-76.
https://doi.org/10.5937/FFR1801067M

Milanov D, Petrović T, Todorović D, Aleksić N, Čabarkapa I. Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals. in Food and Feed Research. 2018;45(1):67-76.
doi:10.5937/FFR1801067M .

Milanov, Dubravka, Petrović, Tamaš, Todorović, Dalibor, Aleksić, Nevenka, Čabarkapa, Ivana, "Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals" in Food and Feed Research, 45, no. 1 (2018):67-76,
https://doi.org/10.5937/FFR1801067M . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Ljubojević, Dragana
AU  - Čabarkapa, Ivana
AU  - Karabasil, Nedjeljko
AU  - Velhner, Maja
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1470
AB  - Bacteria belonging to the genus Salmonella are one of the major foodborne pathogens, primarily due to their health and economic impact. The main sources of human infection are poultry products. Salmonella may enter various stages of the poultry production process in many different ways. Biofilm is just one of the recently recognised risk factors, which significantly contributes to the persistence of these bacteria in poultry premises, hatchery cabinets, transportation vehicles, and food and feed factory environment. The control of Salmonella in poultry production is a highly complex and challenging process, additionally complicated by the fact that to date no efficient methods have been developed to eliminate biofilms or prevent their formation. In vitro investigations revealed that the ability to produce biofilm varies between Salmonella serovars, strains of the same serovar and under various environmental conditions. This paper briefly summarises literature data concerning biofilms produced by Salmonella serovars and their influence on the efficacy of control and eradication strategies in the poultry production chain.
PB  - Eugen Ulmer Gmbh Co, Stuttgart
T2  - European Poultry Science
T1  - Biofilm as risk factor for Salmonella contamination in various stages of poultry production
VL  - 81
DO  - 10.1399/eps.2017.190
ER  - 

@article{
author = "Milanov, Dubravka and Ljubojević, Dragana and Čabarkapa, Ivana and Karabasil, Nedjeljko and Velhner, Maja",
year = "2017",
abstract = "Bacteria belonging to the genus Salmonella are one of the major foodborne pathogens, primarily due to their health and economic impact. The main sources of human infection are poultry products. Salmonella may enter various stages of the poultry production process in many different ways. Biofilm is just one of the recently recognised risk factors, which significantly contributes to the persistence of these bacteria in poultry premises, hatchery cabinets, transportation vehicles, and food and feed factory environment. The control of Salmonella in poultry production is a highly complex and challenging process, additionally complicated by the fact that to date no efficient methods have been developed to eliminate biofilms or prevent their formation. In vitro investigations revealed that the ability to produce biofilm varies between Salmonella serovars, strains of the same serovar and under various environmental conditions. This paper briefly summarises literature data concerning biofilms produced by Salmonella serovars and their influence on the efficacy of control and eradication strategies in the poultry production chain.",
publisher = "Eugen Ulmer Gmbh Co, Stuttgart",
journal = "European Poultry Science",
title = "Biofilm as risk factor for Salmonella contamination in various stages of poultry production",
volume = "81",
doi = "10.1399/eps.2017.190"
}

Milanov, D., Ljubojević, D., Čabarkapa, I., Karabasil, N.,& Velhner, M.. (2017). Biofilm as risk factor for Salmonella contamination in various stages of poultry production. in European Poultry Science
Eugen Ulmer Gmbh Co, Stuttgart., 81.
https://doi.org/10.1399/eps.2017.190

Milanov D, Ljubojević D, Čabarkapa I, Karabasil N, Velhner M. Biofilm as risk factor for Salmonella contamination in various stages of poultry production. in European Poultry Science. 2017;81.
doi:10.1399/eps.2017.190 .

Milanov, Dubravka, Ljubojević, Dragana, Čabarkapa, Ivana, Karabasil, Nedjeljko, Velhner, Maja, "Biofilm as risk factor for Salmonella contamination in various stages of poultry production" in European Poultry Science, 81 (2017),
https://doi.org/10.1399/eps.2017.190 . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Ljubojević, Dragana
AU  - Čabarkapa, Ivana
AU  - Aleksić, Nevenka
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1317
AB  - For decades intensive husbandry has more or less been based on the use of antibiotics in sub-minimum inhibitory concentrations (sub-MIC) aimed at growth promotion. Continuous exposure of animal intestinal microbiota, including opportunistic zoonotic pathogens, to sub-MIC poses a pressure to selection and spread of bacteria strains with developed mechanism of antibiotic resistance. These bacteria may be transferred to people either by direct contact with farm animals or indirectly, via the food chain. Although in the EU a ban on the use of antibiotics as growth promoters was imposed in 2006, in many countries, including the largest producers and consumers of antibiotics in the world, it has yet to be done. Given that we are faced with a global problem of the loss of the efficacy of several antibiotic classes which are available for the treatment of human bacterial infections, it is unacceptable that antibiotic use in husbandry is not under global control. Reduction in antibiotic use in clinical practice in human medicine remains in dispute, but non-therapeutic use in husbandry remains a field in which much can be done to contribute substantially to the extension of antibiotic effectiveness and health care of future generations.
AB  - Intenzivna stočarska proizvodnja se decenijama u većoj ili manjoj meri bazira na upotrebi antibiotika u subinhibitornim koncentracijama za promociju rasta. Kontinuirano izlaganje mikrobiota digestivnog trakta životinja (uključujući i oportunističke zoonotske patogene) sub-inhibitornim koncentracijama antibiotika, predstavlja pritisak na selekciju i širenje sojeva bakterija sa mehanizmima rezistencije na antibiotike. Na ljude se ove bakterije mogu preneti direktnim kontaktom sa farmskim životinjama ili na posredan način, preko lanca ishrane. Iako je u zemljama Evropske unije upotreba antibiotika za promociju rasta životinja zabranjena 2006. godine, u mnogim državama, uključujući najveće proizvođače i potrošače antibiotika u svetu, ova praksa se zadržala do danas. Gubitak efikasnosti antibiotika koji su trenutno na raspolaganju za lečenje bakterijskih infekcija kod ljudi je rastući problem, zbog čega je neprihvatljivo da upotreba antibiotika u stočarskoj proizvodnji nije pod globalnom kontrolom. Klinička praksa u humanoj medicini ostavlja diskutabilan prostor za redukciju primene antibiotika, ali je neterapeutska upotreba u stočarstvu oblast u okviru koje se može značajno doprineti produžavanju veka upotrebljivosti pojedinih klasa antibiotika i očuvanju zdravlja budućih generacija.
PB  - Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad
T2  - Food and Feed Research
T1  - Impact of antibiotics used as growth promoters on bacterial resistance
T1  - Uticaj antibiotika koji se koriste kao stimulatori rasta kod životinja na rezistenciju bakterija
VL  - 43
IS  - 2
SP  - 83
EP  - 92
DO  - 10.5937/FFR1602083M
ER  - 

@article{
author = "Milanov, Dubravka and Ljubojević, Dragana and Čabarkapa, Ivana and Aleksić, Nevenka",
year = "2016",
abstract = "For decades intensive husbandry has more or less been based on the use of antibiotics in sub-minimum inhibitory concentrations (sub-MIC) aimed at growth promotion. Continuous exposure of animal intestinal microbiota, including opportunistic zoonotic pathogens, to sub-MIC poses a pressure to selection and spread of bacteria strains with developed mechanism of antibiotic resistance. These bacteria may be transferred to people either by direct contact with farm animals or indirectly, via the food chain. Although in the EU a ban on the use of antibiotics as growth promoters was imposed in 2006, in many countries, including the largest producers and consumers of antibiotics in the world, it has yet to be done. Given that we are faced with a global problem of the loss of the efficacy of several antibiotic classes which are available for the treatment of human bacterial infections, it is unacceptable that antibiotic use in husbandry is not under global control. Reduction in antibiotic use in clinical practice in human medicine remains in dispute, but non-therapeutic use in husbandry remains a field in which much can be done to contribute substantially to the extension of antibiotic effectiveness and health care of future generations., Intenzivna stočarska proizvodnja se decenijama u većoj ili manjoj meri bazira na upotrebi antibiotika u subinhibitornim koncentracijama za promociju rasta. Kontinuirano izlaganje mikrobiota digestivnog trakta životinja (uključujući i oportunističke zoonotske patogene) sub-inhibitornim koncentracijama antibiotika, predstavlja pritisak na selekciju i širenje sojeva bakterija sa mehanizmima rezistencije na antibiotike. Na ljude se ove bakterije mogu preneti direktnim kontaktom sa farmskim životinjama ili na posredan način, preko lanca ishrane. Iako je u zemljama Evropske unije upotreba antibiotika za promociju rasta životinja zabranjena 2006. godine, u mnogim državama, uključujući najveće proizvođače i potrošače antibiotika u svetu, ova praksa se zadržala do danas. Gubitak efikasnosti antibiotika koji su trenutno na raspolaganju za lečenje bakterijskih infekcija kod ljudi je rastući problem, zbog čega je neprihvatljivo da upotreba antibiotika u stočarskoj proizvodnji nije pod globalnom kontrolom. Klinička praksa u humanoj medicini ostavlja diskutabilan prostor za redukciju primene antibiotika, ali je neterapeutska upotreba u stočarstvu oblast u okviru koje se može značajno doprineti produžavanju veka upotrebljivosti pojedinih klasa antibiotika i očuvanju zdravlja budućih generacija.",
publisher = "Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad",
journal = "Food and Feed Research",
title = "Impact of antibiotics used as growth promoters on bacterial resistance, Uticaj antibiotika koji se koriste kao stimulatori rasta kod životinja na rezistenciju bakterija",
volume = "43",
number = "2",
pages = "83-92",
doi = "10.5937/FFR1602083M"
}

Milanov, D., Ljubojević, D., Čabarkapa, I.,& Aleksić, N.. (2016). Impact of antibiotics used as growth promoters on bacterial resistance. in Food and Feed Research
Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad., 43(2), 83-92.
https://doi.org/10.5937/FFR1602083M

Milanov D, Ljubojević D, Čabarkapa I, Aleksić N. Impact of antibiotics used as growth promoters on bacterial resistance. in Food and Feed Research. 2016;43(2):83-92.
doi:10.5937/FFR1602083M .

Milanov, Dubravka, Ljubojević, Dragana, Čabarkapa, Ivana, Aleksić, Nevenka, "Impact of antibiotics used as growth promoters on bacterial resistance" in Food and Feed Research, 43, no. 2 (2016):83-92,
https://doi.org/10.5937/FFR1602083M . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Mišić, Dušan
AU  - Čabarkapa, Ivana
AU  - Ljubojević, Dragana
AU  - Živkov-Baloš, Milica
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1326
AB  - All natural antibiotics available to modern medicine are products of soil-dwelling bacteria and fungi. In addition, all resistance genes which are being detected in human pathogens existed in soil bacteria even before antibiotics were discovered and brought into use. However, the concentrations of natural antibiotics in soil are usually subinhibitory - insufficient for the selection of resistant subpopulations of microorganisms. The consumption of organic fertilisers for agricultural soil amendment increases proportionally to the consumers' growing demand for organically produced food. Manure originating from industrial pig, cattle and poultry farms is not only the source of nutrients which stimulate the vital functions of soil microorganisms, but also of antibiotics and bacteria harbouring various resistance mechanisms. The application of organic fertilizer leads to disruption of the natural balance between bacterial communities in the soil through several mechanisms, and influences the increase in the prevalence of resistance genes and promotes their horizontal transfer. Whether as-yet-unknown resistance genes in soil bacteria may pose threat to human health if transferred from commensal bacteria in the environment to pathogen species, or migrate to clinical settings via food chain or in some other possible route - remains an open question.
AB  - Svi prirodni antibiotici koji su na raspolaganju savremenoj medicini su produkti bakterija i glivica kojima je zemljište prirodno stanište, a svi geni rezistencije koji se danas ustanovljavaju kod humanih patogena postojali su kod bakterija u zemljištu i pre otkrića i upotrebe antibiotika. Međutim, koncentracije prirodnih antibiotika u zemljištu uobičajeno su na nivou subihnibitornih, a time i nedovoljne za selekciju rezistentnih subpopulacija mikroorganizama. Upotreba organskog đubriva za fertilizaciju poljoprivrednog zemljišta, povećava se srazmerno porastu zahteva potrošača za organski proizvednom hranom. Organsko đubrivo poreklom sa industrijalizovanih farmi svinja, goveda i živine, nije samo izvor nutrijenata koji podstiču životne funkcije mikroorganizama u zemljištu, već i antibiotika i bakterija sa raznovrsnim mehanizmima rezistencije. Dodavanje organskog đubriva preko više mehanizama remeti prirodnu ravnotežu zajednice bakterija u zemljištu, utiče na porast prevalencije gena rezistencije i podstiče njihov horizontalni transfer. Otvoreno je pitanje da li do sada neotkriveni geni rezistencije zemljišnih bakterija mogu biti nova pretnja ljudskom zdravlju ukoliko se prenesu sa komensalnih bakterija iz okruženja na patogene vrste, ili lancem ishrane i drugim mogućim putevima, migriraju u kliničke uslove.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Natural antibiotic resistance genes in soil bacteria and influence of organic fertilisers on their prevalence and horizontal transfer
T1  - Geni rezistencije na antibiotike kod bakterija u zemljištu i uticaj primene organskog đubriva na njihovu prevalenciju i horzontalni transfer
VL  - 9
IS  - 2
SP  - 3
EP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1326
ER  - 

@article{
author = "Milanov, Dubravka and Mišić, Dušan and Čabarkapa, Ivana and Ljubojević, Dragana and Živkov-Baloš, Milica",
year = "2016",
abstract = "All natural antibiotics available to modern medicine are products of soil-dwelling bacteria and fungi. In addition, all resistance genes which are being detected in human pathogens existed in soil bacteria even before antibiotics were discovered and brought into use. However, the concentrations of natural antibiotics in soil are usually subinhibitory - insufficient for the selection of resistant subpopulations of microorganisms. The consumption of organic fertilisers for agricultural soil amendment increases proportionally to the consumers' growing demand for organically produced food. Manure originating from industrial pig, cattle and poultry farms is not only the source of nutrients which stimulate the vital functions of soil microorganisms, but also of antibiotics and bacteria harbouring various resistance mechanisms. The application of organic fertilizer leads to disruption of the natural balance between bacterial communities in the soil through several mechanisms, and influences the increase in the prevalence of resistance genes and promotes their horizontal transfer. Whether as-yet-unknown resistance genes in soil bacteria may pose threat to human health if transferred from commensal bacteria in the environment to pathogen species, or migrate to clinical settings via food chain or in some other possible route - remains an open question., Svi prirodni antibiotici koji su na raspolaganju savremenoj medicini su produkti bakterija i glivica kojima je zemljište prirodno stanište, a svi geni rezistencije koji se danas ustanovljavaju kod humanih patogena postojali su kod bakterija u zemljištu i pre otkrića i upotrebe antibiotika. Međutim, koncentracije prirodnih antibiotika u zemljištu uobičajeno su na nivou subihnibitornih, a time i nedovoljne za selekciju rezistentnih subpopulacija mikroorganizama. Upotreba organskog đubriva za fertilizaciju poljoprivrednog zemljišta, povećava se srazmerno porastu zahteva potrošača za organski proizvednom hranom. Organsko đubrivo poreklom sa industrijalizovanih farmi svinja, goveda i živine, nije samo izvor nutrijenata koji podstiču životne funkcije mikroorganizama u zemljištu, već i antibiotika i bakterija sa raznovrsnim mehanizmima rezistencije. Dodavanje organskog đubriva preko više mehanizama remeti prirodnu ravnotežu zajednice bakterija u zemljištu, utiče na porast prevalencije gena rezistencije i podstiče njihov horizontalni transfer. Otvoreno je pitanje da li do sada neotkriveni geni rezistencije zemljišnih bakterija mogu biti nova pretnja ljudskom zdravlju ukoliko se prenesu sa komensalnih bakterija iz okruženja na patogene vrste, ili lancem ishrane i drugim mogućim putevima, migriraju u kliničke uslove.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Natural antibiotic resistance genes in soil bacteria and influence of organic fertilisers on their prevalence and horizontal transfer, Geni rezistencije na antibiotike kod bakterija u zemljištu i uticaj primene organskog đubriva na njihovu prevalenciju i horzontalni transfer",
volume = "9",
number = "2",
pages = "3-16",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1326"
}

Milanov, D., Mišić, D., Čabarkapa, I., Ljubojević, D.,& Živkov-Baloš, M.. (2016). Natural antibiotic resistance genes in soil bacteria and influence of organic fertilisers on their prevalence and horizontal transfer. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 9(2), 3-16.
https://hdl.handle.net/21.15107/rcub_veterinar_1326

Milanov D, Mišić D, Čabarkapa I, Ljubojević D, Živkov-Baloš M. Natural antibiotic resistance genes in soil bacteria and influence of organic fertilisers on their prevalence and horizontal transfer. in Arhiv veterinarske medicine. 2016;9(2):3-16.
https://hdl.handle.net/21.15107/rcub_veterinar_1326 .

Milanov, Dubravka, Mišić, Dušan, Čabarkapa, Ivana, Ljubojević, Dragana, Živkov-Baloš, Milica, "Natural antibiotic resistance genes in soil bacteria and influence of organic fertilisers on their prevalence and horizontal transfer" in Arhiv veterinarske medicine, 9, no. 2 (2016):3-16,
https://hdl.handle.net/21.15107/rcub_veterinar_1326 .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Velhner, Maja
AU  - Karabasil, Nedjeljko
AU  - Čabarkapa, Ivana
AU  - Suvajdžić, Ljiljana
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1181
AB  - Animal feed is the first link in the food chain and one of the possible source of Salmonella for food producing animals and consequently, humans consuming products of animal origin. The assessment of the importance and role of Salmonella organisms commonly detected in animal feed in epidemic outbreaks of salmonellosis is highly intricate. This is mainly due to the fact that isolates are rarely identified (typed) to the serovar level, thus, the relevant data on both animal feed and food of animal origin are lacking. In the framework of the 2-year project granted by the Ministry of Science and Technological Development of the Republic of Serbia, all Salmonella isolates originating from animal feed were typed to the serovar level in the National Reference Laboratory for Salmonella. Eighteen different serovars have been identified, whereas 15% of all isolates included serovar Montevideo. Frequent isolation of S. ser. Montevideo from animal feed originating from feed mills in our epizootic area (South Bačka and Srem district), encouraged our attempt to summarize and present the available data on the importance of Montevideo serovar in the outbreaks of clinical salmonellosis in humans and to review the reports on individual epidemiological studies aimed at detecting infection sources and establishing relevant facts on emerging antimicrobial resistance of Salmonella. Moreover, this article emphasizes the need and importance of an extensive Salmonella monitoring program at national level, which would encompass all links of the food chain including animal feed and feed processing plants as well.
AB  - Hrana za životinje je prva karika u lancu ishrane i jedan od mogućih izvora Salmonella za životinje, a posredno i ljude koji konzumiraju proizvode životinjskog porekla. Značaj i ulogu salmonela koje se ustanovljavaju u hrani za životinje u pojavi epidemija salmoneloza veoma je teško proceniti, jer se izolati retko tipiziraju do serotipa i pravi podaci nedostaju i za hranu za životinje i za namirnice animalnog porekla. U okviru realizacije projekta finansiranog od strane Ministarstva za nauku i tehnološki razvoj Republike Srbije, tokom dve godine su svi izolati Salmonella spp. iz hrane za životinje potvrđeni i tipizirani do serotipa u Nacionalnoj referentnoj laboratoriji za Salmonella. Identifikovano je 18 različitih serotipova, a 15% svih izolata odnosio se na Salmonella ser. Montevideo. Zbog učestale izolacije Salmonella ser. Montevideo iz hrane za životinje poreklom iz fabrika stočne hrane na našem epizootiološkom području (Južnobački i Sremski okrug), u ovom radu iznosimo dostupne podatke o značaju ovog serotipa u pojavi kliničkih salmoneloza kod ljudi, prikaze pojedinačnih epidemioloških istraživanja u cilju utvrđivanja izvora infekcije i relevantne činjenice o rastućoj antimikrobnoj rezistenciji Salmonella. Takođe, u radu ističemo potrebu za jedinstvenim monitoringom nad salmonelama na nacionalnom nivou, koji će uključiti sve karike u lancu ishrane, počevši od hrane za životinje i objekata za njihovu proizvodnju.
PB  - Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad
T2  - Food and Feed Research
T1  - Epidemiological significance of Salmonella enterica serovar Montevideo and the potential role of feed for their entry into the food chain
T1  - Epidemiološki značaj Salmonella enterica serovar Montevideo i potencijalna uloga hrane za životinje za njen ulazak u lanac ishrane
VL  - 42
IS  - 2
SP  - 155
EP  - 162
DO  - 10.5937/FFR1502155M
ER  - 

@article{
author = "Milanov, Dubravka and Velhner, Maja and Karabasil, Nedjeljko and Čabarkapa, Ivana and Suvajdžić, Ljiljana",
year = "2015",
abstract = "Animal feed is the first link in the food chain and one of the possible source of Salmonella for food producing animals and consequently, humans consuming products of animal origin. The assessment of the importance and role of Salmonella organisms commonly detected in animal feed in epidemic outbreaks of salmonellosis is highly intricate. This is mainly due to the fact that isolates are rarely identified (typed) to the serovar level, thus, the relevant data on both animal feed and food of animal origin are lacking. In the framework of the 2-year project granted by the Ministry of Science and Technological Development of the Republic of Serbia, all Salmonella isolates originating from animal feed were typed to the serovar level in the National Reference Laboratory for Salmonella. Eighteen different serovars have been identified, whereas 15% of all isolates included serovar Montevideo. Frequent isolation of S. ser. Montevideo from animal feed originating from feed mills in our epizootic area (South Bačka and Srem district), encouraged our attempt to summarize and present the available data on the importance of Montevideo serovar in the outbreaks of clinical salmonellosis in humans and to review the reports on individual epidemiological studies aimed at detecting infection sources and establishing relevant facts on emerging antimicrobial resistance of Salmonella. Moreover, this article emphasizes the need and importance of an extensive Salmonella monitoring program at national level, which would encompass all links of the food chain including animal feed and feed processing plants as well., Hrana za životinje je prva karika u lancu ishrane i jedan od mogućih izvora Salmonella za životinje, a posredno i ljude koji konzumiraju proizvode životinjskog porekla. Značaj i ulogu salmonela koje se ustanovljavaju u hrani za životinje u pojavi epidemija salmoneloza veoma je teško proceniti, jer se izolati retko tipiziraju do serotipa i pravi podaci nedostaju i za hranu za životinje i za namirnice animalnog porekla. U okviru realizacije projekta finansiranog od strane Ministarstva za nauku i tehnološki razvoj Republike Srbije, tokom dve godine su svi izolati Salmonella spp. iz hrane za životinje potvrđeni i tipizirani do serotipa u Nacionalnoj referentnoj laboratoriji za Salmonella. Identifikovano je 18 različitih serotipova, a 15% svih izolata odnosio se na Salmonella ser. Montevideo. Zbog učestale izolacije Salmonella ser. Montevideo iz hrane za životinje poreklom iz fabrika stočne hrane na našem epizootiološkom području (Južnobački i Sremski okrug), u ovom radu iznosimo dostupne podatke o značaju ovog serotipa u pojavi kliničkih salmoneloza kod ljudi, prikaze pojedinačnih epidemioloških istraživanja u cilju utvrđivanja izvora infekcije i relevantne činjenice o rastućoj antimikrobnoj rezistenciji Salmonella. Takođe, u radu ističemo potrebu za jedinstvenim monitoringom nad salmonelama na nacionalnom nivou, koji će uključiti sve karike u lancu ishrane, počevši od hrane za životinje i objekata za njihovu proizvodnju.",
publisher = "Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad",
journal = "Food and Feed Research",
title = "Epidemiological significance of Salmonella enterica serovar Montevideo and the potential role of feed for their entry into the food chain, Epidemiološki značaj Salmonella enterica serovar Montevideo i potencijalna uloga hrane za životinje za njen ulazak u lanac ishrane",
volume = "42",
number = "2",
pages = "155-162",
doi = "10.5937/FFR1502155M"
}

Milanov, D., Velhner, M., Karabasil, N., Čabarkapa, I.,& Suvajdžić, L.. (2015). Epidemiological significance of Salmonella enterica serovar Montevideo and the potential role of feed for their entry into the food chain. in Food and Feed Research
Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad., 42(2), 155-162.
https://doi.org/10.5937/FFR1502155M

Milanov D, Velhner M, Karabasil N, Čabarkapa I, Suvajdžić L. Epidemiological significance of Salmonella enterica serovar Montevideo and the potential role of feed for their entry into the food chain. in Food and Feed Research. 2015;42(2):155-162.
doi:10.5937/FFR1502155M .

Milanov, Dubravka, Velhner, Maja, Karabasil, Nedjeljko, Čabarkapa, Ivana, Suvajdžić, Ljiljana, "Epidemiological significance of Salmonella enterica serovar Montevideo and the potential role of feed for their entry into the food chain" in Food and Feed Research, 42, no. 2 (2015):155-162,
https://doi.org/10.5937/FFR1502155M . .