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Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum
dc.creator | Tubić Vukajlović, Jovana | |
dc.creator | Kosanić, Marijana | |
dc.creator | Ranković, Branislav | |
dc.creator | Stanojković, Tatjana | |
dc.creator | Marković, Aleksandra | |
dc.creator | Grujičić, Darko | |
dc.creator | Đelić, Ninoslav | |
dc.creator | Radaković, Milena | |
dc.creator | Milošević-Djordjević, Olivera | |
dc.date.accessioned | 2021-12-22T07:28:21Z | |
dc.date.available | 2021-12-22T07:28:21Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 0014-8237 | |
dc.identifier.uri | https://vet-erinar.vet.bg.ac.rs/handle/123456789/2318 | |
dc.description.abstract | Bioactivity of acetone extract of Leccinum scabrum was evaluated. Antioxidant activity was evaluated by free radical scavenging activity, reducing power and determination of total phenolic content. The microdilution plate method was used to detect antimicrobial activity. Cytotoxic activity was tested by MTT assay. Genotoxic potential was evaluated by comet assay. Results showed a stronger free radical scavenging activity, but the reducing power was less pronounced. The total content of phenols in the extract was 14.91 µg PE/mg. The minimum inhibitory concentration related to the tested microorganisms fluctuated in the range 0.039-10 mg/mL. The most sensitive was Bacillus cereus. A strong cytotoxic activity was revealed with IC50 values ranging between 73.64 and 200.00 µg/mL, among which HeLa was the most sensitive. Results showed that the extract was not genotoxic and it decreased the H2O2-induced GDI values in comparison to the positive control. L. scabrum possesses bioactivities such as antioxidant, antimicrobial, cytotoxic, no genotoxic and protective against H2O2, and in the highest tested concentration it showed a synergistic activity. | |
dc.description.abstract | Studiul prezintă evaluarea activității biologice a extractului în acetonă obținut din Leccinum scabrum. Activitatea antioxidantă a fost evaluată prin determinarea capacității de chelatare a radicalilor liberi, a capacității reducătoare și a conținutului total în fenoli. Activitatea antimicrobiană a fost testată prin metoda microdiluției, activitatea citotoxică prin metoda MTT, iar potențialul genotoxic a fost determinat prin calcularea indexului de deteriorare genetică. Conținutul total de fenoli a fost 14,91 μg PE/mg. Concentrația minimă inhibitorie a variat în intervalul 0,039 - 10 mg/mL. Activitatea citotoxică pronunțată a fost corelată cu valorile IC50 în domeniul 73,64 - 200,00 μg/mL. Rezultatele au arătat că extractul nu este genotoxic și au scăzut valorile indexului de deteriorare genetică-indus de H2O2 , în comparație cu controlul pozitiv. L. scabrum posedă activități antioxidantă, antimicrobiană, citotoxică șı protectoare împotriva H2O2. | |
dc.language | English | |
dc.publisher | Societatea de Științe Farmaceutice din România | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/20094/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200122/RS// | |
dc.rights | openAccess | |
dc.source | Farmacia | |
dc.subject | Acetone extract | |
dc.subject | Biological activities | |
dc.subject | Mushroom Leccinum scabrum | |
dc.title | Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum | |
dc.type | article | en |
dc.rights.license | ARR | |
dc.citation.volume | 69 | |
dc.citation.issue | 5 | |
dc.citation.spage | 974 | |
dc.citation.epage | 979 | |
dc.citation.rank | M23 | |
dc.identifier.wos | 000713322700022 | |
dc.identifier.doi | 10.31925/FARMACIA.2021.5.22 | |
dc.identifier.scopus | 2-s2.0-85120774323 | |
dc.identifier.fulltext | http://veterinar.vet.bg.ac.rs/bitstream/id/6415/art-22-Vukajlovic_Milosevic-Djordjevic_974-979.pdf | |
dc.type.version | publishedVersion |