Immunohistochemical investigation of the Bursa of Fabricius in chickens experimentally infected by eimeria tenella

Abstract

The aim of this investigation was to detect and examine the distribution of the CD3-T lymphocyte cell population of the Bursa Fabricius in chickens experimentally infected with Eimeria tenella. Slices of archived samples of Bursa were examined using the of direct peroxidase (DP) method. CD3-T lymphocytes were detected with human anti-CD3 antibody. They were localized in the cortico-medullary layer of Bursa follicles in uninfected chickens. The immunoreactivity of bursal tissue was directly correlated with the stage of development of E. tenella, with the highest intensity in the epithelium of the folds and in the layers of follicles between 3 and 5 days after the infection, predominantly in the cortico-medullary layer of the follicles. These results demonstrate the existence of an early cellular immunological response to infection with E. tenella.

Ispitivanja u ovom radu su imala za cilj detekciju i utvrđivanje distribucije CD3-T limfocitne populacije u Fabricijusovoj burzi pilića veštački inficiranih sa E. tenella. Za izvođenje imunohistohemijskih ispitivanja korišćeni su parafinski isečci burzi, koji su nakon standardne procedure obrađeni metodom direktne peroksidaze (DP). CD3-T limfocitna populacija detektovana humanim CD3 antitelom, lokalizovana je u kortiko-medularnom sloju folikula burze, kod neinficiranih pilića. Imunoreaktivnost tkiva burze u direktnoj je zavisnosti od stadijuma razvoja E. tenella, a najintenzivnija je u epitelu plike i slojevima folikula između 3. i 5. dana posle infekcije, sa dominacijom u kortiko-medularnom sloju folikula. Rezultati dobijeni u okviru navedenih ispitivanja dokazuju postojanje ranog imunološkog odgovora na infekciju prouzrokovanu sa E. tenella, koji je celularnog karaktera.