TY  - JOUR
AU  - Pajić, Marko
AU  - Todorović, Dalibor
AU  - Knežević, Slobodan
AU  - Prunić, Bojana
AU  - Velhner, Maja
AU  - Ostojić Andrić, Dušica
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2902
AB  - Coccidiosis is a significant poultry disease caused by the Eimeria species. This study aims to
determine the prevalence of Eimeria spp. on broiler farms in Vojvodina, along with the identification
of parasite species, and assess the implemented biosecurity measures. The study was conducted on
100 broiler chicken farms (28 small-sized; 34 medium-sized; 38 large-sized farms) from June 2018 to
December 2021. One pooled sample of faeces was collected from three to six-week-old chickens from
each farm, and assessment of biosecurity measures was carried out using a questionnaire. Using the
PCR method, DNA of Eimeria was found in 59 samples (59%), while 41 samples (41%) were negative.
Four species of Eimeria were identified, and their prevalence was the following: E. acervulina (37%),
E. maxima (17%), E. mitis (25%) and E. tenella (48%). A significant difference (p < 0.05) was established
in the number of oocysts in flocks from small-sized farms compared to medium-sized farms. It was
found that regular implementation of disinfection, disinsection and deratisation measures, as well as
all the biosecurity measures, can significantly reduce the occurrence of coccidiosis. These results will
help to develop better strategies for the control and prevention of coccidiosis on farms.
PB  - MDPI
T2  - Life
T1  - Molecular Investigation of Eimeria Species in Broiler Farms in the Province of Vojvodina, Serbia
VL  - 13
IS  - 4
SP  - 1
EP  - 13
DO  - 10.3390/life13041039
ER  - 

@article{
author = "Pajić, Marko and Todorović, Dalibor and Knežević, Slobodan and Prunić, Bojana and Velhner, Maja and Ostojić Andrić, Dušica and Stanimirović, Zoran",
year = "2023",
abstract = "Coccidiosis is a significant poultry disease caused by the Eimeria species. This study aims to
determine the prevalence of Eimeria spp. on broiler farms in Vojvodina, along with the identification
of parasite species, and assess the implemented biosecurity measures. The study was conducted on
100 broiler chicken farms (28 small-sized; 34 medium-sized; 38 large-sized farms) from June 2018 to
December 2021. One pooled sample of faeces was collected from three to six-week-old chickens from
each farm, and assessment of biosecurity measures was carried out using a questionnaire. Using the
PCR method, DNA of Eimeria was found in 59 samples (59%), while 41 samples (41%) were negative.
Four species of Eimeria were identified, and their prevalence was the following: E. acervulina (37%),
E. maxima (17%), E. mitis (25%) and E. tenella (48%). A significant difference (p < 0.05) was established
in the number of oocysts in flocks from small-sized farms compared to medium-sized farms. It was
found that regular implementation of disinfection, disinsection and deratisation measures, as well as
all the biosecurity measures, can significantly reduce the occurrence of coccidiosis. These results will
help to develop better strategies for the control and prevention of coccidiosis on farms.",
publisher = "MDPI",
journal = "Life",
title = "Molecular Investigation of Eimeria Species in Broiler Farms in the Province of Vojvodina, Serbia",
volume = "13",
number = "4",
pages = "1-13",
doi = "10.3390/life13041039"
}

Pajić, M., Todorović, D., Knežević, S., Prunić, B., Velhner, M., Ostojić Andrić, D.,& Stanimirović, Z.. (2023). Molecular Investigation of Eimeria Species in Broiler Farms in the Province of Vojvodina, Serbia. in Life
MDPI., 13(4), 1-13.
https://doi.org/10.3390/life13041039

Pajić M, Todorović D, Knežević S, Prunić B, Velhner M, Ostojić Andrić D, Stanimirović Z. Molecular Investigation of Eimeria Species in Broiler Farms in the Province of Vojvodina, Serbia. in Life. 2023;13(4):1-13.
doi:10.3390/life13041039 .

Pajić, Marko, Todorović, Dalibor, Knežević, Slobodan, Prunić, Bojana, Velhner, Maja, Ostojić Andrić, Dušica, Stanimirović, Zoran, "Molecular Investigation of Eimeria Species in Broiler Farms in the Province of Vojvodina, Serbia" in Life, 13, no. 4 (2023):1-13,
https://doi.org/10.3390/life13041039 . .

TY  - JOUR
AU  - Prunić, Bojana
AU  - Milanov, Dubravka
AU  - Velhner, Maja
AU  - Pajić, Marko
AU  - Pavlović, Ljiljana
AU  - Mišić, Dušan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1399
AB  - Introduction: Novel molecular techniques applied in biotechnology research have provided sound evidence on clonal persistence of distinct serovars of Salmonella in feed factory environments, over long periods of time (months, even years), which can be responsible for repeated in-house contamination of final products. In this study, we examined the possibility of clonal persistence of isolates of three Salmonella serovars that have been repeatedly identified in animal feed samples from three feed factories throughout a two-year period. Methodology: The isolates Salmonella enterica serovars Tennessee (n = 7), Montevideo (n = 8), and Infantis (n = 4) were tested for genetic diversity using pulsed-field gel electrophoresis (PFGE) and multicellular behavior patterns by applying the Congo red agar test. Results: SpeI and XbaI macro-restriction profiles indicated that isolates S. Montevideo and S. Infantis were identical, whereas isolates of S. Tennessee demonstrated greater genetic diversity, although the genetic differences did not exceed 10%. All Salmonella serovars demonstrated the ability to produce predominant matrix compounds essential for biofilm formation, curli fimbriae and cellulose. Conclusions: The identification of identical clones of S. Montevideo and S. Infantis, as well as the minor genetic diversity of S. Tennessee, which have been repeatedly isolated from animal feed in three production plants throughout a two-year period, indirectly suggests the possibility of their persistence in feed factory environments. Their ability to express the key biofilm matrix components further supports this hypothesis.
PB  - J Infection Developing Countries, Tramaniglio
T2  - Journal of Infection in Developing Countries
T1  - Clonal persistence of Salmonella enterica serovars Montevideo, Tennessee, and Infantis in feed factories
VL  - 10
IS  - 6
SP  - 662
EP  - 666
DO  - 10.3855/jidc.7313
ER  - 

@article{
author = "Prunić, Bojana and Milanov, Dubravka and Velhner, Maja and Pajić, Marko and Pavlović, Ljiljana and Mišić, Dušan",
year = "2016",
abstract = "Introduction: Novel molecular techniques applied in biotechnology research have provided sound evidence on clonal persistence of distinct serovars of Salmonella in feed factory environments, over long periods of time (months, even years), which can be responsible for repeated in-house contamination of final products. In this study, we examined the possibility of clonal persistence of isolates of three Salmonella serovars that have been repeatedly identified in animal feed samples from three feed factories throughout a two-year period. Methodology: The isolates Salmonella enterica serovars Tennessee (n = 7), Montevideo (n = 8), and Infantis (n = 4) were tested for genetic diversity using pulsed-field gel electrophoresis (PFGE) and multicellular behavior patterns by applying the Congo red agar test. Results: SpeI and XbaI macro-restriction profiles indicated that isolates S. Montevideo and S. Infantis were identical, whereas isolates of S. Tennessee demonstrated greater genetic diversity, although the genetic differences did not exceed 10%. All Salmonella serovars demonstrated the ability to produce predominant matrix compounds essential for biofilm formation, curli fimbriae and cellulose. Conclusions: The identification of identical clones of S. Montevideo and S. Infantis, as well as the minor genetic diversity of S. Tennessee, which have been repeatedly isolated from animal feed in three production plants throughout a two-year period, indirectly suggests the possibility of their persistence in feed factory environments. Their ability to express the key biofilm matrix components further supports this hypothesis.",
publisher = "J Infection Developing Countries, Tramaniglio",
journal = "Journal of Infection in Developing Countries",
title = "Clonal persistence of Salmonella enterica serovars Montevideo, Tennessee, and Infantis in feed factories",
volume = "10",
number = "6",
pages = "662-666",
doi = "10.3855/jidc.7313"
}

Prunić, B., Milanov, D., Velhner, M., Pajić, M., Pavlović, L.,& Mišić, D.. (2016). Clonal persistence of Salmonella enterica serovars Montevideo, Tennessee, and Infantis in feed factories. in Journal of Infection in Developing Countries
J Infection Developing Countries, Tramaniglio., 10(6), 662-666.
https://doi.org/10.3855/jidc.7313

Prunić B, Milanov D, Velhner M, Pajić M, Pavlović L, Mišić D. Clonal persistence of Salmonella enterica serovars Montevideo, Tennessee, and Infantis in feed factories. in Journal of Infection in Developing Countries. 2016;10(6):662-666.
doi:10.3855/jidc.7313 .

Prunić, Bojana, Milanov, Dubravka, Velhner, Maja, Pajić, Marko, Pavlović, Ljiljana, Mišić, Dušan, "Clonal persistence of Salmonella enterica serovars Montevideo, Tennessee, and Infantis in feed factories" in Journal of Infection in Developing Countries, 10, no. 6 (2016):662-666,
https://doi.org/10.3855/jidc.7313 . .