Maletić, Jelena

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  • Maletić, Jelena (17)

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Kretanje nalaza salmonela kod živine na beogradskom epizootiološkom području u periodu 2017 - 2024. godine

Zdravković, Nemanja; Radanović, Oliver; Spalević, Ljiljana; Maletić, Jelena; Ninković, Milan; Marjanović, Đorđe; Medić, Dragana

(Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze, 2024)

TY  - CONF
AU  - Zdravković, Nemanja
AU  - Radanović, Oliver
AU  - Spalević, Ljiljana
AU  - Maletić, Jelena
AU  - Ninković, Milan
AU  - Marjanović, Đorđe
AU  - Medić, Dragana
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3855
AB  - Салмонелозе живине су скуп болести коју узрокују Грам-негативне бактерије из 
породице Enterobacteriaceae и врсте Salmonella enterica. Од око 2500 описаних 
серотипова ове врсте, око 10 % изоловано из живине. Посебан значај салмонеле 
добијају својим карактером, јер поједини серотипови могу угрозити здравље 
људи, доводећи до гастроинтестиналних токсоинфекција услед конзумације 
живинских производа. Описан је вертикални пренос трансоваријалним путем на 
потомство, али и хоризонтални и то најчешће излучивањем у фецесу. Као највећа 
опасност по људско здравље, издваја се налаз салмонела у месу, јајима или на 
љусци живинских јаја. Поједина живина може бити заражена салмонелама а да 
не показује клиничке знаке болести, али чине значајан извор ширења заразе, док 
клинички симптоми варирају од дигестивних проблема (бели пролив, 
маладсорпција), до бактеријемије и угинућа.
Циљ овог испитивања је био ретроспективно прикаже присуство бактерија рода 
Salmonella enterica subspecies enterica разних серотопова, код живине на 
београдском епизоотиолошком подручју. Испитивањем су обухваћени узорци:
ембрионирана јаја, лешеви једнодневних пилића, фецес бројлера и носиља, у 
последњих шест година. Салмонеле су изоловане стандардним микробиолошким 
методама праћено серолошком типизацијом.
У испитиваном број позитивних узорака салмонела живине се кретао од 879 што 
је забележено у 2017. преко 65 2018.године, док су значајно нижи налази 
забележени током 2016. и 2021. године са 7 позитивних узорака, док је 2022. 
године забележено 4, а током 2023. године 2 позитивна узорка. Изоловани 
серотипови у наведеном периоду су S. Enteritidis, S. Infantis, S. Mbandaka, S. 
Senftenberg, S. Typhimurium, S. Agona, S. Taksony и S. Shiongwe. Највише 
позитивних узорака је установљено у фецесу носиља, затим фецесу бројлера, 
лешевима једнодневних пилића и ембрионираним јајима.
Потпуно искорењивање салмонела из производње је тежак циљ због присуства 
великог броја серотипова салмонела и различитих извора заразе, али је зато 
потребно донети правилну стратегију контроле за спречавање ризика од 
контаминације. Превенција је најбољи алат за контролу салмонела: хигијена, 
биосигурност и тамо где је изводљиво - вакцинација. Велика је одговорност на 
узгајивачима на примени постојећих и унапређењу нових стандарда.
AB  - Poultry salmonellosеs are group of diseases caused by Gram-negative bacteria from the
Enterobacteriaceae and species Salmonella enterica. Of about 2500 serotypes of this
species, about 10% are isolated from poultry. Special significance of salmonella comes
from their character, as some serotypes can endanger human health leading to
gastrointestinal toxin infections due to the consumption of poultry products. Vertical
transmission is described by transovarian route to offspring, but also horizontal
transmission, the most often by faecal secretion. The greatest risk for human well-being
is the finding of Salmonella in poultry meat, in the eggs and on the eggshells. Some poultry
can be infected with Salmonella without showing any clinical signs of the disease, but
nevertheless being a significant source of infection, while clinical symptoms range from
digestive problems (white diarrhoea, malabsorption), to bacteraemia and death. The aim
of this paper is to retrospective presence of bacteria Salmonella enterica subspecies
enterica in poultry in the Belgrade epizootiological area. Observation included samples
of: embryonic eggs, day-old chicken cadavers, broiler and laying hens faeces samples
investigated in six-year period. Salmonellae were isolated by standard microbiological
methods followed by serological typing.
In the examined number of positive samples of poultry salmonella ranged from 879
recorded in 2017 to 65 in 2018, while significantly lower findings were recorded in 2016
and 2021 with 7 positive samples, while 4 were recorded in 2022, and during 2023, 2
positive samples. The serotypes isolated in the mentioned period are S. Enteritidis, S.
Infantis, S. Mbandaka, S. Senftenberg, S. Typhimurium, S. Agona, S. Taksony and S.
Shiongwe. The most positive samples were found in the feces of laying hens, followed by
the feces of broilers, carcasses of one-day-old chickens and embryonated eggs.
Complete eradication of Salmonella from production is a difficult goal because of the
presence of a large number of Salmonella serotypes and various sources of infection, but
a proper control strategy must therefore be executed to prevent the risk of contamination.
Prevention is the best tool for controlling Salmonella: hygiene, biosecurity and
vaccination. It is a great responsibility of the breeders to apply the existing standards and
to improve the new ones.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024
T1  - Kretanje nalaza salmonela kod živine na beogradskom epizootiološkom području u periodu 2017 - 2024. godine
T1  - Trends in salmonella poultry findings in Belgrade epizoological area during 2017 - 2024 period
SP  - 170
EP  - 171
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3855
ER  - 
@conference{
author = "Zdravković, Nemanja and Radanović, Oliver and Spalević, Ljiljana and Maletić, Jelena and Ninković, Milan and Marjanović, Đorđe and Medić, Dragana",
year = "2024",
abstract = "Салмонелозе живине су скуп болести коју узрокују Грам-негативне бактерије из 
породице Enterobacteriaceae и врсте Salmonella enterica. Од око 2500 описаних 
серотипова ове врсте, око 10 % изоловано из живине. Посебан значај салмонеле 
добијају својим карактером, јер поједини серотипови могу угрозити здравље 
људи, доводећи до гастроинтестиналних токсоинфекција услед конзумације 
живинских производа. Описан је вертикални пренос трансоваријалним путем на 
потомство, али и хоризонтални и то најчешће излучивањем у фецесу. Као највећа 
опасност по људско здравље, издваја се налаз салмонела у месу, јајима или на 
љусци живинских јаја. Поједина живина може бити заражена салмонелама а да 
не показује клиничке знаке болести, али чине значајан извор ширења заразе, док 
клинички симптоми варирају од дигестивних проблема (бели пролив, 
маладсорпција), до бактеријемије и угинућа.
Циљ овог испитивања је био ретроспективно прикаже присуство бактерија рода 
Salmonella enterica subspecies enterica разних серотопова, код живине на 
београдском епизоотиолошком подручју. Испитивањем су обухваћени узорци:
ембрионирана јаја, лешеви једнодневних пилића, фецес бројлера и носиља, у 
последњих шест година. Салмонеле су изоловане стандардним микробиолошким 
методама праћено серолошком типизацијом.
У испитиваном број позитивних узорака салмонела живине се кретао од 879 што 
је забележено у 2017. преко 65 2018.године, док су значајно нижи налази 
забележени током 2016. и 2021. године са 7 позитивних узорака, док је 2022. 
године забележено 4, а током 2023. године 2 позитивна узорка. Изоловани 
серотипови у наведеном периоду су S. Enteritidis, S. Infantis, S. Mbandaka, S. 
Senftenberg, S. Typhimurium, S. Agona, S. Taksony и S. Shiongwe. Највише 
позитивних узорака је установљено у фецесу носиља, затим фецесу бројлера, 
лешевима једнодневних пилића и ембрионираним јајима.
Потпуно искорењивање салмонела из производње је тежак циљ због присуства 
великог броја серотипова салмонела и различитих извора заразе, али је зато 
потребно донети правилну стратегију контроле за спречавање ризика од 
контаминације. Превенција је најбољи алат за контролу салмонела: хигијена, 
биосигурност и тамо где је изводљиво - вакцинација. Велика је одговорност на 
узгајивачима на примени постојећих и унапређењу нових стандарда., Poultry salmonellosеs are group of diseases caused by Gram-negative bacteria from the
Enterobacteriaceae and species Salmonella enterica. Of about 2500 serotypes of this
species, about 10% are isolated from poultry. Special significance of salmonella comes
from their character, as some serotypes can endanger human health leading to
gastrointestinal toxin infections due to the consumption of poultry products. Vertical
transmission is described by transovarian route to offspring, but also horizontal
transmission, the most often by faecal secretion. The greatest risk for human well-being
is the finding of Salmonella in poultry meat, in the eggs and on the eggshells. Some poultry
can be infected with Salmonella without showing any clinical signs of the disease, but
nevertheless being a significant source of infection, while clinical symptoms range from
digestive problems (white diarrhoea, malabsorption), to bacteraemia and death. The aim
of this paper is to retrospective presence of bacteria Salmonella enterica subspecies
enterica in poultry in the Belgrade epizootiological area. Observation included samples
of: embryonic eggs, day-old chicken cadavers, broiler and laying hens faeces samples
investigated in six-year period. Salmonellae were isolated by standard microbiological
methods followed by serological typing.
In the examined number of positive samples of poultry salmonella ranged from 879
recorded in 2017 to 65 in 2018, while significantly lower findings were recorded in 2016
and 2021 with 7 positive samples, while 4 were recorded in 2022, and during 2023, 2
positive samples. The serotypes isolated in the mentioned period are S. Enteritidis, S.
Infantis, S. Mbandaka, S. Senftenberg, S. Typhimurium, S. Agona, S. Taksony and S.
Shiongwe. The most positive samples were found in the feces of laying hens, followed by
the feces of broilers, carcasses of one-day-old chickens and embryonated eggs.
Complete eradication of Salmonella from production is a difficult goal because of the
presence of a large number of Salmonella serotypes and various sources of infection, but
a proper control strategy must therefore be executed to prevent the risk of contamination.
Prevention is the best tool for controlling Salmonella: hygiene, biosecurity and
vaccination. It is a great responsibility of the breeders to apply the existing standards and
to improve the new ones.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024",
title = "Kretanje nalaza salmonela kod živine na beogradskom epizootiološkom području u periodu 2017 - 2024. godine, Trends in salmonella poultry findings in Belgrade epizoological area during 2017 - 2024 period",
pages = "170-171",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3855"
}
Zdravković, N., Radanović, O., Spalević, L., Maletić, J., Ninković, M., Marjanović, Đ.,& Medić, D.. (2024). Kretanje nalaza salmonela kod živine na beogradskom epizootiološkom području u periodu 2017 - 2024. godine. in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 170-171.
https://hdl.handle.net/21.15107/rcub_veterinar_3855
Zdravković N, Radanović O, Spalević L, Maletić J, Ninković M, Marjanović Đ, Medić D. Kretanje nalaza salmonela kod živine na beogradskom epizootiološkom području u periodu 2017 - 2024. godine. in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024. 2024;:170-171.
https://hdl.handle.net/21.15107/rcub_veterinar_3855 .
Zdravković, Nemanja, Radanović, Oliver, Spalević, Ljiljana, Maletić, Jelena, Ninković, Milan, Marjanović, Đorđe, Medić, Dragana, "Kretanje nalaza salmonela kod živine na beogradskom epizootiološkom području u periodu 2017 - 2024. godine" in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024 (2024):170-171,
https://hdl.handle.net/21.15107/rcub_veterinar_3855 .

Fowl Adenovirus Infection-Potential Cause of a Suppressed Humoral Immune Response of Broilers to Newcastle Disease Vaccination

Maletić, Jelena; Spalević, Ljiljana; Kureljušić, Branislav; Veljović, Ljubiša; Maksimović-Zorić, Jelena; Maletić, Milan; Milićević, Vesna

(Sciendo, 2023)

TY  - JOUR
AU  - Maletić, Jelena
AU  - Spalević, Ljiljana
AU  - Kureljušić, Branislav
AU  - Veljović, Ljubiša
AU  - Maksimović-Zorić, Jelena
AU  - Maletić, Milan
AU  - Milićević, Vesna
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2815
AB  - Fowl adenovirus infections have a significant economic impact, especially in the production of broilers. It is considered the leading cause of three syndromes: Adenoviral gizzard erosions and ulcerations, inclusion body hepatitis, and hepatitis-hydropericardium syndrome. A critical feature of this virus is its immunosuppressive effect, via suppressing humoral and cellular immunity. In this study, we examined the humoral immune response after administration of the Newcastle disease vaccine in broiler flocks with previously confirmed seroconversion against Fowl adenovirus. The study was conducted on 5 farms. A total of 220 chickens, five weeks of age, showing no clinical signs of the disease, were included in this study. The control group consisted of 20 chickens from a negative farm. Chickens were vaccinated with commercially available live NDV vaccines between 11 and 13 days of life. ELISA determined the presence of specific antibodies against FAdV in a total of 130/200 (65%) blood sera. Depending on the farm, seroprevalence ranged from 30-100%. The presence of specific antibodies against NDV was determined three weeks after vaccination using the hemagglutination inhibition assay. A positive hemagglutination inhibition (HI) titer (≥ 16) was found in 41/200 (20.5%) sera, which was significantly less compared to the control farm, where a positive HI titer was found in 20/20 (100%) sera. The results of our study indicate the immunosuppressive effect of FAdV in subclinically infected birds and highlight the need for its diagnosis, prevention, and control
PB  - Sciendo
T2  - Acta Veterinaria
T1  - Fowl Adenovirus Infection-Potential Cause of a Suppressed Humoral Immune Response of Broilers to Newcastle Disease Vaccination
VL  - 73
IS  - 1
SP  - 133
EP  - 142
DO  - 10.2478/acve-2023-0010
ER  - 
@article{
author = "Maletić, Jelena and Spalević, Ljiljana and Kureljušić, Branislav and Veljović, Ljubiša and Maksimović-Zorić, Jelena and Maletić, Milan and Milićević, Vesna",
year = "2023",
abstract = "Fowl adenovirus infections have a significant economic impact, especially in the production of broilers. It is considered the leading cause of three syndromes: Adenoviral gizzard erosions and ulcerations, inclusion body hepatitis, and hepatitis-hydropericardium syndrome. A critical feature of this virus is its immunosuppressive effect, via suppressing humoral and cellular immunity. In this study, we examined the humoral immune response after administration of the Newcastle disease vaccine in broiler flocks with previously confirmed seroconversion against Fowl adenovirus. The study was conducted on 5 farms. A total of 220 chickens, five weeks of age, showing no clinical signs of the disease, were included in this study. The control group consisted of 20 chickens from a negative farm. Chickens were vaccinated with commercially available live NDV vaccines between 11 and 13 days of life. ELISA determined the presence of specific antibodies against FAdV in a total of 130/200 (65%) blood sera. Depending on the farm, seroprevalence ranged from 30-100%. The presence of specific antibodies against NDV was determined three weeks after vaccination using the hemagglutination inhibition assay. A positive hemagglutination inhibition (HI) titer (≥ 16) was found in 41/200 (20.5%) sera, which was significantly less compared to the control farm, where a positive HI titer was found in 20/20 (100%) sera. The results of our study indicate the immunosuppressive effect of FAdV in subclinically infected birds and highlight the need for its diagnosis, prevention, and control",
publisher = "Sciendo",
journal = "Acta Veterinaria",
title = "Fowl Adenovirus Infection-Potential Cause of a Suppressed Humoral Immune Response of Broilers to Newcastle Disease Vaccination",
volume = "73",
number = "1",
pages = "133-142",
doi = "10.2478/acve-2023-0010"
}
Maletić, J., Spalević, L., Kureljušić, B., Veljović, L., Maksimović-Zorić, J., Maletić, M.,& Milićević, V.. (2023). Fowl Adenovirus Infection-Potential Cause of a Suppressed Humoral Immune Response of Broilers to Newcastle Disease Vaccination. in Acta Veterinaria
Sciendo., 73(1), 133-142.
https://doi.org/10.2478/acve-2023-0010
Maletić J, Spalević L, Kureljušić B, Veljović L, Maksimović-Zorić J, Maletić M, Milićević V. Fowl Adenovirus Infection-Potential Cause of a Suppressed Humoral Immune Response of Broilers to Newcastle Disease Vaccination. in Acta Veterinaria. 2023;73(1):133-142.
doi:10.2478/acve-2023-0010 .
Maletić, Jelena, Spalević, Ljiljana, Kureljušić, Branislav, Veljović, Ljubiša, Maksimović-Zorić, Jelena, Maletić, Milan, Milićević, Vesna, "Fowl Adenovirus Infection-Potential Cause of a Suppressed Humoral Immune Response of Broilers to Newcastle Disease Vaccination" in Acta Veterinaria, 73, no. 1 (2023):133-142,
https://doi.org/10.2478/acve-2023-0010 . .

Seroprevalence of Schmallenberg virus in sheep in Belgrade epizootic area

Veljović, Ljubiša; Maksimović Zorić, Jelena; Glišić, Dimitrije; Nišavić, Jakov; Maletić, Jelena; Milićević, Vesna

(University of Belgrade - Faculty of Veterinary Medicine, 2023)

TY  - JOUR
AU  - Veljović, Ljubiša
AU  - Maksimović Zorić, Jelena
AU  - Glišić, Dimitrije
AU  - Nišavić, Jakov
AU  - Maletić, Jelena
AU  - Milićević, Vesna
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3704
AB  - Schmallenberg disease is an infectious disease of cattle, sheep, and goats of viral
aetiology caused by the Schmallenberg virus that belongs to the family Bunyaviridae and
the genus Ortobunyavirus. Schmallenberg disease is a vector-borne disease transmitted by
midges from the genus Culicoides but also by other hematophagous insects. The disease
has a seasonal character and most often occurs in the warm months, from late spring
to autumn. In this study, we investigated the seroprevalence of Schmallenberg disease
in sheep in the epizootic area of Belgrade in the period from 2017 to 2022. A total of
600 sheep serums from the serum bank were tested, 100 serum samples from each year.
Sera were tested by commercial ELISA test for the detection of specifi c antibodies
against the Schmallenberg virus. The results showed an average annual seroprevalence
of 24.5% in sheep in the Belgrade area in these six years with an increasing trend
predicted for the next years.
PB  - University of Belgrade - Faculty of Veterinary Medicine
T2  - Acta Veterinaria
T1  - Seroprevalence of Schmallenberg virus in sheep in Belgrade epizootic area
VL  - 73
IS  - 4
SP  - 502
EP  - 510
DO  - 10.2478/acve-2023-0038
ER  - 
@article{
author = "Veljović, Ljubiša and Maksimović Zorić, Jelena and Glišić, Dimitrije and Nišavić, Jakov and Maletić, Jelena and Milićević, Vesna",
year = "2023",
abstract = "Schmallenberg disease is an infectious disease of cattle, sheep, and goats of viral
aetiology caused by the Schmallenberg virus that belongs to the family Bunyaviridae and
the genus Ortobunyavirus. Schmallenberg disease is a vector-borne disease transmitted by
midges from the genus Culicoides but also by other hematophagous insects. The disease
has a seasonal character and most often occurs in the warm months, from late spring
to autumn. In this study, we investigated the seroprevalence of Schmallenberg disease
in sheep in the epizootic area of Belgrade in the period from 2017 to 2022. A total of
600 sheep serums from the serum bank were tested, 100 serum samples from each year.
Sera were tested by commercial ELISA test for the detection of specifi c antibodies
against the Schmallenberg virus. The results showed an average annual seroprevalence
of 24.5% in sheep in the Belgrade area in these six years with an increasing trend
predicted for the next years.",
publisher = "University of Belgrade - Faculty of Veterinary Medicine",
journal = "Acta Veterinaria",
title = "Seroprevalence of Schmallenberg virus in sheep in Belgrade epizootic area",
volume = "73",
number = "4",
pages = "502-510",
doi = "10.2478/acve-2023-0038"
}
Veljović, L., Maksimović Zorić, J., Glišić, D., Nišavić, J., Maletić, J.,& Milićević, V.. (2023). Seroprevalence of Schmallenberg virus in sheep in Belgrade epizootic area. in Acta Veterinaria
University of Belgrade - Faculty of Veterinary Medicine., 73(4), 502-510.
https://doi.org/10.2478/acve-2023-0038
Veljović L, Maksimović Zorić J, Glišić D, Nišavić J, Maletić J, Milićević V. Seroprevalence of Schmallenberg virus in sheep in Belgrade epizootic area. in Acta Veterinaria. 2023;73(4):502-510.
doi:10.2478/acve-2023-0038 .
Veljović, Ljubiša, Maksimović Zorić, Jelena, Glišić, Dimitrije, Nišavić, Jakov, Maletić, Jelena, Milićević, Vesna, "Seroprevalence of Schmallenberg virus in sheep in Belgrade epizootic area" in Acta Veterinaria, 73, no. 4 (2023):502-510,
https://doi.org/10.2478/acve-2023-0038 . .

Efficiency of two protocols of resynchronization of estrus and ovulation in high-producing dairy cows at peak lactation

Đorđević, Milan; Blagojević, Jovan; Radinović, Miodrag; Cincović, Marko; Maletić, Jelena; Kreszinger, Mario; Maletić, Milan

(Sciendo, 2022)

TY  - JOUR
AU  - Đorđević, Milan
AU  - Blagojević, Jovan
AU  - Radinović, Miodrag
AU  - Cincović, Marko
AU  - Maletić, Jelena
AU  - Kreszinger, Mario
AU  - Maletić, Milan
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2832
AB  - The reproductive efficiency of the cows was monitored after two resynchronization protocols: Ovsynch (OVS) and Double Ovsynch (DOS). The research initially included 70 HF cows who entered the first synchronization protocol - Presynch. Cows that did not conceive after the first synchronization were divided into two groups and introduced to two resynchronization protocols. In the first group of cows (n=35), the DOS protocol began with the application of GnRH on day 22 after the Presynch TAI (Timed Artificial Insemination), and seven days later pregnancy check-up was done and PGF2α was applied only to non-pregnant cows (n=23), which remained in the study. In the second group of cows, the OVS protocol started on day 32 after Presynch TAI only in non-pregnant animals (n=20). Progesterone (P4) concentration was determined at the time of application of GnRH1, PGF2α and GnRH2 in both groups of cows, and then 30 days after Resynch TAI, ultrasound pregnancy diagnosis was done. A higher percentage of pregnant cows were recorded in the OVS group compared to the DOS group (45% and 35%, respectively). The concentration of P4 in the serum of cows in the DOS group during the first measurement (GnRH1) was significantly higher than the value in cows that did not conceive (p<0.05), while in the third measurement (GnRH2) the average concentration of P4 in conceiving cows was significantly lower (p<0.001) compared to cows that did not coincive. The open days period was significantly longer in pregnant cows that were resynchronized using the DOS protocol compared to cows from the OVS protocol. In conclusion, the OVS protocol of estrus resynchronization in dairy cows proved to be more successful than the DOS protocol. However, considering the advantages the OVS, it is needed to determine which day of the sexual cycle is the best to start resynchronization.
PB  - Sciendo
T2  - Acta Veterinaria
T1  - Efficiency of two protocols of resynchronization of estrus and ovulation in high-producing dairy cows at peak lactation
VL  - 72
VL  - 3
SP  - 362
SP  - 374
DO  - 10.2478/acve-2022-0029
ER  - 
@article{
author = "Đorđević, Milan and Blagojević, Jovan and Radinović, Miodrag and Cincović, Marko and Maletić, Jelena and Kreszinger, Mario and Maletić, Milan",
year = "2022",
abstract = "The reproductive efficiency of the cows was monitored after two resynchronization protocols: Ovsynch (OVS) and Double Ovsynch (DOS). The research initially included 70 HF cows who entered the first synchronization protocol - Presynch. Cows that did not conceive after the first synchronization were divided into two groups and introduced to two resynchronization protocols. In the first group of cows (n=35), the DOS protocol began with the application of GnRH on day 22 after the Presynch TAI (Timed Artificial Insemination), and seven days later pregnancy check-up was done and PGF2α was applied only to non-pregnant cows (n=23), which remained in the study. In the second group of cows, the OVS protocol started on day 32 after Presynch TAI only in non-pregnant animals (n=20). Progesterone (P4) concentration was determined at the time of application of GnRH1, PGF2α and GnRH2 in both groups of cows, and then 30 days after Resynch TAI, ultrasound pregnancy diagnosis was done. A higher percentage of pregnant cows were recorded in the OVS group compared to the DOS group (45% and 35%, respectively). The concentration of P4 in the serum of cows in the DOS group during the first measurement (GnRH1) was significantly higher than the value in cows that did not conceive (p<0.05), while in the third measurement (GnRH2) the average concentration of P4 in conceiving cows was significantly lower (p<0.001) compared to cows that did not coincive. The open days period was significantly longer in pregnant cows that were resynchronized using the DOS protocol compared to cows from the OVS protocol. In conclusion, the OVS protocol of estrus resynchronization in dairy cows proved to be more successful than the DOS protocol. However, considering the advantages the OVS, it is needed to determine which day of the sexual cycle is the best to start resynchronization.",
publisher = "Sciendo",
journal = "Acta Veterinaria",
title = "Efficiency of two protocols of resynchronization of estrus and ovulation in high-producing dairy cows at peak lactation",
volume = "72, 3",
pages = "362-374",
doi = "10.2478/acve-2022-0029"
}
Đorđević, M., Blagojević, J., Radinović, M., Cincović, M., Maletić, J., Kreszinger, M.,& Maletić, M.. (2022). Efficiency of two protocols of resynchronization of estrus and ovulation in high-producing dairy cows at peak lactation. in Acta Veterinaria
Sciendo., 72, 362.
https://doi.org/10.2478/acve-2022-0029
Đorđević M, Blagojević J, Radinović M, Cincović M, Maletić J, Kreszinger M, Maletić M. Efficiency of two protocols of resynchronization of estrus and ovulation in high-producing dairy cows at peak lactation. in Acta Veterinaria. 2022;72:362.
doi:10.2478/acve-2022-0029 .
Đorđević, Milan, Blagojević, Jovan, Radinović, Miodrag, Cincović, Marko, Maletić, Jelena, Kreszinger, Mario, Maletić, Milan, "Efficiency of two protocols of resynchronization of estrus and ovulation in high-producing dairy cows at peak lactation" in Acta Veterinaria, 72 (2022):362,
https://doi.org/10.2478/acve-2022-0029 . .
1

Prevalencija virusnih uzročnika proliva kod teladi na farmama muznih krava u Srbiji

Milićević, Vesna; Maletić, Jelena; Kureljušić, Branislav; Maksimović Zorić, Jelena; Veljović, Ljubiša; Glišić, Dimitrije; Jezdimirović, Nemanja; Maletić, Milan

(Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze, 2022)

TY  - CONF
AU  - Milićević, Vesna
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
AU  - Maksimović Zorić, Jelena
AU  - Veljović, Ljubiša
AU  - Glišić, Dimitrije
AU  - Jezdimirović, Nemanja
AU  - Maletić, Milan
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3178
AB  - Неонатална дијареја телади је један од најважнијих здравствених проблема са којима се суочава савремена говедарска производња и која узрокује значајне економске губитке због високог морбидитета и морталитета. Иако болест могу изазвати инфективни и неинфективни фактори, различити патогени, укључујући вирусе, бактерије и протозое, сматрају се примарним узрочницима овог обољења. Ипак, коинфекција инфективних агенаса је најчешћи узрок дијареје код телади. Овом студијом су истраживани главни вирусни патогени који изазивају неонаталну дијареју телади у Србији. Шездесет четири узорка фецеса сакупљена су од телади са дијарејом, до 3 месеца старости, са 13 фарми музних крава и тестирана на главне вирусне инфективне агенсе, укључујући говеђи ротавирус (БоРоВ), говеђи коронавирус (БоКоВ) и вирус вирусне дијареје говеда (БВДВ) коришћењем ланчане реакције полимеразе - ПЦР. Резултати ПЦР-а су показали да је 15.4% фарми било позитивно на БВДВ и БоКоВ, док је на 76.9% фарми откривен БоРоВ. Истовремена циркулација БВДВ, БоРоВ и БоКоВ детектована је на 1 фарми, а циркулација БВДВ и БоРоВ на другој. Поред тога, коинфекција БВДВ и БоРоВ је потврђена код 2 телади, док је коинфекција БоКоВ и БоРоВ откривена код 1 телета са исте фарме. Код једног телета са друге фарме откривена је коинфекција БВДВ и БоРоВ. Ови резултати су показали да је инфекција БоРоВ код телади најзаступљенија без обзира на примену вакцинације у контроли пролива, а углавном због велике разноликости сојева ротавируса типа А. Резултати ове студије могу помоћи да се скрене пажња на важност и потребу тачне дијагнозе неонаталне дијареје телади и преиспитивања спроведених мера за њену контролу.
AB  - Neonatal calf diarrhoea (NCD) is one of the most important health issues facing the modern dairy industry and causes substantial economic losses due to high morbidity and mortality. Although the disease can be caused by infectious and non-infectious factors, various pathogens, including viruses, bacteria and protozoa, are considered as the primary causes of NDT. However, co-infection of these infectious agents is most common in calves with diarrhoea. The present study investigated the main viral pathogens causing NCD among calves in Serbia. Sixty-four faecal samples were collected from diarrheic calves from 13 dairy farms and tested for major viral infectious agents, including bovine rotavirus (BoRoV), bovine coronavirus (BoCoV), and bovine viral diarrhoea virus (BVDV) using polymerase chain reaction (PCR). The PCR results showed that 15.4% of farms were positive for BVDV and BoCoV, while at 76.9% of farms BoRoV was detected. Simultaneous circulation of BVDV, BoRoV and BoCoV was detected on 1 farm, and circulation of BVDV and BoRoV on another one. Furthermore, coinfection of BVDV and BoRoV was confirmed in 2 calves, whereas coinfection of BoCoV and BoRoV was detected in 1 calf from the same farm. BVDV and BoRoV coinfection was detected in one calf from another farm. These results showed that BoRoV infection in neonatal calves is the most prevalent despite applied vaccination, mainly due to the high diversity of Rotavirus A strains. The results of this study may help to draw attention to the importance and the need for accurate diagnosis of NCD and reconsideration of implemented measures to control NCD.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022
T1  - Prevalencija virusnih uzročnika proliva kod teladi na farmama muznih krava u Srbiji
T1  - Prevalence of viral diarrhea in calves on dairy farms in Serbia
SP  - 82
EP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3178
ER  - 
@conference{
author = "Milićević, Vesna and Maletić, Jelena and Kureljušić, Branislav and Maksimović Zorić, Jelena and Veljović, Ljubiša and Glišić, Dimitrije and Jezdimirović, Nemanja and Maletić, Milan",
year = "2022",
abstract = "Неонатална дијареја телади је један од најважнијих здравствених проблема са којима се суочава савремена говедарска производња и која узрокује значајне економске губитке због високог морбидитета и морталитета. Иако болест могу изазвати инфективни и неинфективни фактори, различити патогени, укључујући вирусе, бактерије и протозое, сматрају се примарним узрочницима овог обољења. Ипак, коинфекција инфективних агенаса је најчешћи узрок дијареје код телади. Овом студијом су истраживани главни вирусни патогени који изазивају неонаталну дијареју телади у Србији. Шездесет четири узорка фецеса сакупљена су од телади са дијарејом, до 3 месеца старости, са 13 фарми музних крава и тестирана на главне вирусне инфективне агенсе, укључујући говеђи ротавирус (БоРоВ), говеђи коронавирус (БоКоВ) и вирус вирусне дијареје говеда (БВДВ) коришћењем ланчане реакције полимеразе - ПЦР. Резултати ПЦР-а су показали да је 15.4% фарми било позитивно на БВДВ и БоКоВ, док је на 76.9% фарми откривен БоРоВ. Истовремена циркулација БВДВ, БоРоВ и БоКоВ детектована је на 1 фарми, а циркулација БВДВ и БоРоВ на другој. Поред тога, коинфекција БВДВ и БоРоВ је потврђена код 2 телади, док је коинфекција БоКоВ и БоРоВ откривена код 1 телета са исте фарме. Код једног телета са друге фарме откривена је коинфекција БВДВ и БоРоВ. Ови резултати су показали да је инфекција БоРоВ код телади најзаступљенија без обзира на примену вакцинације у контроли пролива, а углавном због велике разноликости сојева ротавируса типа А. Резултати ове студије могу помоћи да се скрене пажња на важност и потребу тачне дијагнозе неонаталне дијареје телади и преиспитивања спроведених мера за њену контролу., Neonatal calf diarrhoea (NCD) is one of the most important health issues facing the modern dairy industry and causes substantial economic losses due to high morbidity and mortality. Although the disease can be caused by infectious and non-infectious factors, various pathogens, including viruses, bacteria and protozoa, are considered as the primary causes of NDT. However, co-infection of these infectious agents is most common in calves with diarrhoea. The present study investigated the main viral pathogens causing NCD among calves in Serbia. Sixty-four faecal samples were collected from diarrheic calves from 13 dairy farms and tested for major viral infectious agents, including bovine rotavirus (BoRoV), bovine coronavirus (BoCoV), and bovine viral diarrhoea virus (BVDV) using polymerase chain reaction (PCR). The PCR results showed that 15.4% of farms were positive for BVDV and BoCoV, while at 76.9% of farms BoRoV was detected. Simultaneous circulation of BVDV, BoRoV and BoCoV was detected on 1 farm, and circulation of BVDV and BoRoV on another one. Furthermore, coinfection of BVDV and BoRoV was confirmed in 2 calves, whereas coinfection of BoCoV and BoRoV was detected in 1 calf from the same farm. BVDV and BoRoV coinfection was detected in one calf from another farm. These results showed that BoRoV infection in neonatal calves is the most prevalent despite applied vaccination, mainly due to the high diversity of Rotavirus A strains. The results of this study may help to draw attention to the importance and the need for accurate diagnosis of NCD and reconsideration of implemented measures to control NCD.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022",
title = "Prevalencija virusnih uzročnika proliva kod teladi na farmama muznih krava u Srbiji, Prevalence of viral diarrhea in calves on dairy farms in Serbia",
pages = "82-83",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3178"
}
Milićević, V., Maletić, J., Kureljušić, B., Maksimović Zorić, J., Veljović, L., Glišić, D., Jezdimirović, N.,& Maletić, M.. (2022). Prevalencija virusnih uzročnika proliva kod teladi na farmama muznih krava u Srbiji. in XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 82-83.
https://hdl.handle.net/21.15107/rcub_veterinar_3178
Milićević V, Maletić J, Kureljušić B, Maksimović Zorić J, Veljović L, Glišić D, Jezdimirović N, Maletić M. Prevalencija virusnih uzročnika proliva kod teladi na farmama muznih krava u Srbiji. in XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022. 2022;:82-83.
https://hdl.handle.net/21.15107/rcub_veterinar_3178 .
Milićević, Vesna, Maletić, Jelena, Kureljušić, Branislav, Maksimović Zorić, Jelena, Veljović, Ljubiša, Glišić, Dimitrije, Jezdimirović, Nemanja, Maletić, Milan, "Prevalencija virusnih uzročnika proliva kod teladi na farmama muznih krava u Srbiji" in XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022 (2022):82-83,
https://hdl.handle.net/21.15107/rcub_veterinar_3178 .

Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens

Maletić, Jelena; Kureljušić, Branislav; Spalević, Ljiljana; Vučićević, Ivana; Veljović, Ljubiša; Milovanović, Bojan; Milićević, Vesna

(2022)

TY  - CONF
AU  - Maletić, Jelena
AU  - Kureljušić, Branislav
AU  - Spalević, Ljiljana
AU  - Vučićević, Ivana
AU  - Veljović, Ljubiša
AU  - Milovanović, Bojan
AU  - Milićević, Vesna
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2866
AB  - Marek’s disease virus and avian leucosis virus are causes of contagious, immunosuppressive, and oncogenic diseases. Many
reports have described single virus infection, but detailed description of co-infection with Marek’s disease virus and avian
leucosis virus has been provided in a few previous reports. The aim of this study was to determine the cause of health disorders
in a flock of the Banat Naked Neck hens at the age of 11 weeks. Clinical examination of the flock showed signs of cachexia,
apathy, dropped wings, nervous symptoms in the form of bilateral leg paresis, sporadic diarrhoea, and the mortality reaching
20%. Post-mortem examinations showed the following changes: chronic pseudomembranous typhlitis, intestinal ascaridiasis,
splenomegaly, granulomatous pneumonia, multiple yellowish caseous foci in the liver, and white tumour masses in the heart.
Histopathological examination of the spleen, proventriculus and heart tissue revealed polymorphic infiltrates, while
granulomatous inflammation was observed in the lung and liver. Gomori methenamine silver stain method revealed septate and
arborized hyphae resembling the Aspergillus spp. fungi in the liver and lung granulomas. Bacteriological examination of faeces
revealed the presence of Escherichia coli, and parasitological examination of the small intestine determined the presence of
oocyst of Eimeria acervulina and Eimeria tenella in the caecum. The genomes of Marek's disease virus and avian leucosis
virus were determined by polymerase chain reaction in tissue samples. In this case, specific pathological lesions, as well as
molecular finding confirmed co-infection with Marek’s disease virus and avian leucosis virus.
AB  - Virus Marekove bolesti i virus ptičje leukoze uzrokuju zarazne, imunosupresivne i onkogene bolesti. Mnoga izvješća
opisuju infekciju pojedinim virusom, ali tek mali broj izvješća podrobnije opisuje koinfekciju virusom Marekove bolesti i
virusom ptičje leukoze. Cilj ovog istraživanja bio je utvrditi uzrok zdravstvenih poremećaja u jatu kokoši pasmine Banatski
gološijan u dobi od 11 tjedana. Klinički pregled jata pokazao je znakove kaheksije, apatije, spuštena krila, živčane
simptome u obliku pareze nogu, sporadični proljev te 20%-tnu smrtnost. Postmortem pregledi pokazali su sljedeće
promjene: kronični pseudomembranski tiflitis, crijevnu askaridijazu, splenomegaliju, granulomatoznu pneumoniju,
višestruka žućkasta sirasta žarišta u jetri i bijele tumorske tvorbe u srcu. Patohistološki pregled tkivnih uzoraka slezene,
proventrikla i srca otkrio je polimorfne infiltrate, dok je u plućima i jetri zabilježena granulomatozna upala. Metenamin
srebrno bojenje po Gomoriju otkrilo je septirane i arborizirane hife nalik gljivama Aspergillus spp. u jetrenim i plućnim
granulomima. Bakteriološke pretrage fecesa pokazale su prisutnost Escherichia coli, dok je parazitološka pretraga tankog
crijeva utvrdila prisutnost oocita Eimeria acervulina te Eimeria tenella u cekumu. PCR-om su genomi virusa Marekove
bolesti i virusa ptičje leukoze utvrđeni u tkivnim uzorcima. U ovom istraživanju su specifične patološke promjene i
molekularni nalazi potvrdili koinfekciju virusom Marekove bolesti i virusom ptičje leukoze.
C3  - XIV Symposium poultry days 2022 With International Participation, Croatia, Poreč, May 11‐14, 2022
T1  - Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens
T1  - Koinfekcija virusom Marekove bolesti i virusom ptičje leukoze kod kokoši pasmine banatski gološijan
SP  - 210
EP  - 217
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2866
ER  - 
@conference{
author = "Maletić, Jelena and Kureljušić, Branislav and Spalević, Ljiljana and Vučićević, Ivana and Veljović, Ljubiša and Milovanović, Bojan and Milićević, Vesna",
year = "2022",
abstract = "Marek’s disease virus and avian leucosis virus are causes of contagious, immunosuppressive, and oncogenic diseases. Many
reports have described single virus infection, but detailed description of co-infection with Marek’s disease virus and avian
leucosis virus has been provided in a few previous reports. The aim of this study was to determine the cause of health disorders
in a flock of the Banat Naked Neck hens at the age of 11 weeks. Clinical examination of the flock showed signs of cachexia,
apathy, dropped wings, nervous symptoms in the form of bilateral leg paresis, sporadic diarrhoea, and the mortality reaching
20%. Post-mortem examinations showed the following changes: chronic pseudomembranous typhlitis, intestinal ascaridiasis,
splenomegaly, granulomatous pneumonia, multiple yellowish caseous foci in the liver, and white tumour masses in the heart.
Histopathological examination of the spleen, proventriculus and heart tissue revealed polymorphic infiltrates, while
granulomatous inflammation was observed in the lung and liver. Gomori methenamine silver stain method revealed septate and
arborized hyphae resembling the Aspergillus spp. fungi in the liver and lung granulomas. Bacteriological examination of faeces
revealed the presence of Escherichia coli, and parasitological examination of the small intestine determined the presence of
oocyst of Eimeria acervulina and Eimeria tenella in the caecum. The genomes of Marek's disease virus and avian leucosis
virus were determined by polymerase chain reaction in tissue samples. In this case, specific pathological lesions, as well as
molecular finding confirmed co-infection with Marek’s disease virus and avian leucosis virus., Virus Marekove bolesti i virus ptičje leukoze uzrokuju zarazne, imunosupresivne i onkogene bolesti. Mnoga izvješća
opisuju infekciju pojedinim virusom, ali tek mali broj izvješća podrobnije opisuje koinfekciju virusom Marekove bolesti i
virusom ptičje leukoze. Cilj ovog istraživanja bio je utvrditi uzrok zdravstvenih poremećaja u jatu kokoši pasmine Banatski
gološijan u dobi od 11 tjedana. Klinički pregled jata pokazao je znakove kaheksije, apatije, spuštena krila, živčane
simptome u obliku pareze nogu, sporadični proljev te 20%-tnu smrtnost. Postmortem pregledi pokazali su sljedeće
promjene: kronični pseudomembranski tiflitis, crijevnu askaridijazu, splenomegaliju, granulomatoznu pneumoniju,
višestruka žućkasta sirasta žarišta u jetri i bijele tumorske tvorbe u srcu. Patohistološki pregled tkivnih uzoraka slezene,
proventrikla i srca otkrio je polimorfne infiltrate, dok je u plućima i jetri zabilježena granulomatozna upala. Metenamin
srebrno bojenje po Gomoriju otkrilo je septirane i arborizirane hife nalik gljivama Aspergillus spp. u jetrenim i plućnim
granulomima. Bakteriološke pretrage fecesa pokazale su prisutnost Escherichia coli, dok je parazitološka pretraga tankog
crijeva utvrdila prisutnost oocita Eimeria acervulina te Eimeria tenella u cekumu. PCR-om su genomi virusa Marekove
bolesti i virusa ptičje leukoze utvrđeni u tkivnim uzorcima. U ovom istraživanju su specifične patološke promjene i
molekularni nalazi potvrdili koinfekciju virusom Marekove bolesti i virusom ptičje leukoze.",
journal = "XIV Symposium poultry days 2022 With International Participation, Croatia, Poreč, May 11‐14, 2022",
title = "Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens, Koinfekcija virusom Marekove bolesti i virusom ptičje leukoze kod kokoši pasmine banatski gološijan",
pages = "210-217",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2866"
}
Maletić, J., Kureljušić, B., Spalević, L., Vučićević, I., Veljović, L., Milovanović, B.,& Milićević, V.. (2022). Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens. in XIV Symposium poultry days 2022 With International Participation, Croatia, Poreč, May 11‐14, 2022, 210-217.
https://hdl.handle.net/21.15107/rcub_veterinar_2866
Maletić J, Kureljušić B, Spalević L, Vučićević I, Veljović L, Milovanović B, Milićević V. Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens. in XIV Symposium poultry days 2022 With International Participation, Croatia, Poreč, May 11‐14, 2022. 2022;:210-217.
https://hdl.handle.net/21.15107/rcub_veterinar_2866 .
Maletić, Jelena, Kureljušić, Branislav, Spalević, Ljiljana, Vučićević, Ivana, Veljović, Ljubiša, Milovanović, Bojan, Milićević, Vesna, "Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens" in XIV Symposium poultry days 2022 With International Participation, Croatia, Poreč, May 11‐14, 2022 (2022):210-217,
https://hdl.handle.net/21.15107/rcub_veterinar_2866 .

Biosigurnost i zdravstvena zaštita na komercijalnim farmama svinja

Bojkovski, Jovan; Becskei, Zsolt; Kureljušić, Branislav; Pavlović, Ivan; Zdravković, Nemanja; Prodanov-Radulović, Jasna; Vasiljević, Teodora; Angjelovski, Branko; Plut, Jan; Dobrosavljević, Ivan; Maletić, Jelena; Đedović, Suzana; Stanković, Branislav

(Beograd : Udruženje veterinara praktičara Srbije, 2022)

TY  - CONF
AU  - Bojkovski, Jovan
AU  - Becskei, Zsolt
AU  - Kureljušić, Branislav
AU  - Pavlović, Ivan
AU  - Zdravković, Nemanja
AU  - Prodanov-Radulović, Jasna
AU  - Vasiljević, Teodora
AU  - Angjelovski, Branko
AU  - Plut, Jan
AU  - Dobrosavljević, Ivan
AU  - Maletić, Jelena
AU  - Đedović, Suzana
AU  - Stanković, Branislav
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2890
AB  - Na komercijalnim faramama svinja, zdravstvena zaštita pojedinačnih životinja sve više gubi na značaju. U današnjim uslovima, kada je prusutna velika koncentracija životinja na relativno malom prostoru, takvo stanje zahteva zdravstvenu zaštitu na nivou cele farme. Poznata je važnost biosigurnosnih mera u svinjarstvu, koje imaju za cilj da spreče unošenje patogena na farmu i širenje infekcije unutar
same farme. Naime, prisutnost različitih uzgojnih bolesti, baketrijske ili virusne etiologije, dovodi do pada proizvodnih rezultata i zahteva dodatni napor zaposlenih na farmi, uz povećan utrošak lekova, što posledično umanjuje ekonomsku dobit farme. Prihvaćeno je da ključni element, u sprovođenju biosigurnosnih mera, predstavlja spremnost zaposlenih na farmi da sprovedu mere koje će sa visokom sigurnošću obezbediti
zaštitu od unošenja i širenja patogena na farmi. Sprovođenje ovih mera zahteva poznavanje zdravstvenog statusa svinja. Jedan od najčešćih faktora rizika je kupovina nerastova, nazimica ili odlučene prasadi nepoznatog zdravstvenog statusa. Stabilizovanju
zdravstvenog statusa svinja, pored već pomenutih mera, doprinose i programi vakcinacije. Promocija dobrog zdravlja svinja na komercijalnim farmama obuhvata primenu
sistema profilaktičkih mera, pored dobrog odabira rase, osmišljenosti veličine zapata i
načina primene biotehnoloških mera u procesu proizvodnje.
AB  - On commercial pig farms, the health care of individual animals is
losing importance. In today’s production conditions, a large concentration of animals is
relatively present in a small area. This situation requires health care at the farm level.
It is well known that biosecurity measures in pig farming are necessary and aimed to
prevent introduction of pathogens on a commercial farm and also for preventing spreading the infection within the farm itself. Namely, the presence of different breeding
diseases of bacterial or viral etiology leads to a decrease in production. That requires
additional efforts of farm employees and the use of medicines, and this situation ultimately reduces the economic profit of the farm. It is recognized that key elements in implementing biosecurity are the willingness of farm employees to implement measures
that will provide high security with protection against the introduction and spread of
pathogens on the farm. Implementation of the measures requires knowledge of the pigs’
health status on the farm. One of the most common risk factors on the farm is purchasing boars, sows or piglets raised with unknown health status. The promotion of good
pig health on commercial farms involves usage of different prophylactic measures, in
addition to the good breeding selection, the sense of the size of plot, and the way how
biotechnological measures are applied in the pig production process.
PB  - Beograd : Udruženje veterinara praktičara Srbije
C3  - IV Simpozijum sa međunarodnim učešćem - Zdravstvena zaštita i reprodukcija papkara, kopitara, živine i mesojeda, Beograd, 8 - 9. april 2022
T1  - Biosigurnost i zdravstvena zaštita na komercijalnim farmama svinja
SP  - 79
EP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2890
ER  - 
@conference{
author = "Bojkovski, Jovan and Becskei, Zsolt and Kureljušić, Branislav and Pavlović, Ivan and Zdravković, Nemanja and Prodanov-Radulović, Jasna and Vasiljević, Teodora and Angjelovski, Branko and Plut, Jan and Dobrosavljević, Ivan and Maletić, Jelena and Đedović, Suzana and Stanković, Branislav",
year = "2022",
abstract = "Na komercijalnim faramama svinja, zdravstvena zaštita pojedinačnih životinja sve više gubi na značaju. U današnjim uslovima, kada je prusutna velika koncentracija životinja na relativno malom prostoru, takvo stanje zahteva zdravstvenu zaštitu na nivou cele farme. Poznata je važnost biosigurnosnih mera u svinjarstvu, koje imaju za cilj da spreče unošenje patogena na farmu i širenje infekcije unutar
same farme. Naime, prisutnost različitih uzgojnih bolesti, baketrijske ili virusne etiologije, dovodi do pada proizvodnih rezultata i zahteva dodatni napor zaposlenih na farmi, uz povećan utrošak lekova, što posledično umanjuje ekonomsku dobit farme. Prihvaćeno je da ključni element, u sprovođenju biosigurnosnih mera, predstavlja spremnost zaposlenih na farmi da sprovedu mere koje će sa visokom sigurnošću obezbediti
zaštitu od unošenja i širenja patogena na farmi. Sprovođenje ovih mera zahteva poznavanje zdravstvenog statusa svinja. Jedan od najčešćih faktora rizika je kupovina nerastova, nazimica ili odlučene prasadi nepoznatog zdravstvenog statusa. Stabilizovanju
zdravstvenog statusa svinja, pored već pomenutih mera, doprinose i programi vakcinacije. Promocija dobrog zdravlja svinja na komercijalnim farmama obuhvata primenu
sistema profilaktičkih mera, pored dobrog odabira rase, osmišljenosti veličine zapata i
načina primene biotehnoloških mera u procesu proizvodnje., On commercial pig farms, the health care of individual animals is
losing importance. In today’s production conditions, a large concentration of animals is
relatively present in a small area. This situation requires health care at the farm level.
It is well known that biosecurity measures in pig farming are necessary and aimed to
prevent introduction of pathogens on a commercial farm and also for preventing spreading the infection within the farm itself. Namely, the presence of different breeding
diseases of bacterial or viral etiology leads to a decrease in production. That requires
additional efforts of farm employees and the use of medicines, and this situation ultimately reduces the economic profit of the farm. It is recognized that key elements in implementing biosecurity are the willingness of farm employees to implement measures
that will provide high security with protection against the introduction and spread of
pathogens on the farm. Implementation of the measures requires knowledge of the pigs’
health status on the farm. One of the most common risk factors on the farm is purchasing boars, sows or piglets raised with unknown health status. The promotion of good
pig health on commercial farms involves usage of different prophylactic measures, in
addition to the good breeding selection, the sense of the size of plot, and the way how
biotechnological measures are applied in the pig production process.",
publisher = "Beograd : Udruženje veterinara praktičara Srbije",
journal = "IV Simpozijum sa međunarodnim učešćem - Zdravstvena zaštita i reprodukcija papkara, kopitara, živine i mesojeda, Beograd, 8 - 9. april 2022",
title = "Biosigurnost i zdravstvena zaštita na komercijalnim farmama svinja",
pages = "79-94",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2890"
}
Bojkovski, J., Becskei, Z., Kureljušić, B., Pavlović, I., Zdravković, N., Prodanov-Radulović, J., Vasiljević, T., Angjelovski, B., Plut, J., Dobrosavljević, I., Maletić, J., Đedović, S.,& Stanković, B.. (2022). Biosigurnost i zdravstvena zaštita na komercijalnim farmama svinja. in IV Simpozijum sa međunarodnim učešćem - Zdravstvena zaštita i reprodukcija papkara, kopitara, živine i mesojeda, Beograd, 8 - 9. april 2022
Beograd : Udruženje veterinara praktičara Srbije., 79-94.
https://hdl.handle.net/21.15107/rcub_veterinar_2890
Bojkovski J, Becskei Z, Kureljušić B, Pavlović I, Zdravković N, Prodanov-Radulović J, Vasiljević T, Angjelovski B, Plut J, Dobrosavljević I, Maletić J, Đedović S, Stanković B. Biosigurnost i zdravstvena zaštita na komercijalnim farmama svinja. in IV Simpozijum sa međunarodnim učešćem - Zdravstvena zaštita i reprodukcija papkara, kopitara, živine i mesojeda, Beograd, 8 - 9. april 2022. 2022;:79-94.
https://hdl.handle.net/21.15107/rcub_veterinar_2890 .
Bojkovski, Jovan, Becskei, Zsolt, Kureljušić, Branislav, Pavlović, Ivan, Zdravković, Nemanja, Prodanov-Radulović, Jasna, Vasiljević, Teodora, Angjelovski, Branko, Plut, Jan, Dobrosavljević, Ivan, Maletić, Jelena, Đedović, Suzana, Stanković, Branislav, "Biosigurnost i zdravstvena zaštita na komercijalnim farmama svinja" in IV Simpozijum sa međunarodnim učešćem - Zdravstvena zaštita i reprodukcija papkara, kopitara, živine i mesojeda, Beograd, 8 - 9. april 2022 (2022):79-94,
https://hdl.handle.net/21.15107/rcub_veterinar_2890 .

Assessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceive

Bajagić, Bojana; Mrkun, Janko; Kirovski, Danijela; Savić, Đorđe; Budimir, Draženko; Maletić, Jelena; Maletić, Milan

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2021)

TY  - JOUR
AU  - Bajagić, Bojana
AU  - Mrkun, Janko
AU  - Kirovski, Danijela
AU  - Savić, Đorđe
AU  - Budimir, Draženko
AU  - Maletić, Jelena
AU  - Maletić, Milan
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2327
AB  - diagnoses and their accuracy to predict pregnancy. The study was performed on 51
postpartum cross breed dairy cows with pathological puerperium. Artificial insemination
(AI) was done in spontaneous estrus. Endometrium samples were taken by cytotape
(CT), cervical mucus (CM) samples by Metricheck and smears for crystallization degree
were obtained at AI day. Blood serum samples for progesterone (P4) concentration
determination were taken at AI day and 17 days later. Cows were examined by
ultrasound 35 days after AI to confirm pregnancy. Percent (%) of polymorphnuclear
leukocytes (PMNs) taken by CT were expressed by ROC curve (with cut-off point set
at 1%). Out of the total of 51 cows involved in the study, 13.73% true positive and
41.17% true negative cows were found (54.90%). The content of CM sampled with
Metricheck was altered in 11 cows (21.57%), and one of them was pregnant (1.96%).
An average macroscopic CM value for pregnant cows was 0.06±0.24 vs. 0.39±0.79
for non-pregnant (p<0.05). An average value of CM crystallization in pregnant cows
was 2.50±1.04 vs. 1.85±1.15 in non-pregnant (p<0.05). P4 concentration was not
statistically different at AI day, but was statistically significantly higher 17 days later
in pregnant cows (p = 0.002). CT alterations may be a reliable diagnostic tool for
SCE diagnosis, while macroscopic CM alterations may be used for CE detection. P4
determination is beneficial in pregnancy diagnosis while CM crystallization degree is
helpful in determination of best time for AI.
AB  - Cilj studije bio je procena različitih metoda za dijagnostiku supkliničkog endometritisa
(SKE) i njihove pouzdanosti za predviđanje graviditeta. U studiju je bila uključena 51
postpartalna mlečna krava mešane rase sa patološkim puerperijem. Veštačko osemenjavanje
(VO) rađeno je u spontanom estrusu. Na dan VO uzeti su uzorci endometrijuma
dobijeni cytotape (CT) tehnikom, kao i uzorci cervikalne sluzi (CS) dobijeni
upotrebom Metričeka. Razmaz CS, urađen isti dan, korišten je za ispitivanje stepena
kristalizacije. Uzorci krvi za određivanje koncentracije progesterona (P4) uzeti su na
dan VO i 17 dana kasnije. Krave su pregledane ultrazvukom 35 dana nakon VO radi
potvrđivanja graviditeta. Procenat (%) polimorfonuklearnih leukocita (PMNs) dobijenih CT tehnikom je izražen ROC krivom (granična vrednost je određena na 1%).
Od ukupno 54,90% krava uključenih u studiju, utvrđeno je 13,73% stvarno pozitivnih
i 41,17% stvarno negativnih krava. Izmenjen sadržaj CS uzorkovane Metričekom
utvrđen je kod jedanaest krava (21,57%), ali samo jedna (1,96%) je bila gravidna. Prosečna
makroskopska vrednost CS kod gravidnih krava je bila 0,06±0,24 u odnosu na
0,39±0,79 kod negravidnih (p<0,05). Prosečna vrednost kristalizacije CS kod gravidnih
krava je bila 2,50±1,04 u odnosu na 1,85±1,15 kod negravidnih (p<0,05). Koncentracija
P4 nije se statistički razlikovala na dan VO, ali je bila statistički značajno
veća 17 dana kasnije kod gravidnih životinja (p = 0.002). Promene utvrđene CT mogu
biti indikator SKE, a promene CS dobijene Metričekom mogu biti indikator kliničkog
endometritisa. Određivanje P4 korisno je u dijagnostici graviditeta, a kristalizacija CS
može se koristiti kao pomoćni metod za određivanje najpovoljnijeg vremena za VO.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria-Beograd
T1  - Assessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceive
T1  - Procena različitih dijagnostičkih metoda za identifikaciju supkliničkog endometritisa mlečnih krava sa patološkim puerperijumom i njihova pouzdanost u predikciji graviditeta
VL  - 71
IS  - 4
SP  - 462
EP  - 476
DO  - 10.2478/acve-2021-0038
ER  - 
@article{
author = "Bajagić, Bojana and Mrkun, Janko and Kirovski, Danijela and Savić, Đorđe and Budimir, Draženko and Maletić, Jelena and Maletić, Milan",
year = "2021",
abstract = "diagnoses and their accuracy to predict pregnancy. The study was performed on 51
postpartum cross breed dairy cows with pathological puerperium. Artificial insemination
(AI) was done in spontaneous estrus. Endometrium samples were taken by cytotape
(CT), cervical mucus (CM) samples by Metricheck and smears for crystallization degree
were obtained at AI day. Blood serum samples for progesterone (P4) concentration
determination were taken at AI day and 17 days later. Cows were examined by
ultrasound 35 days after AI to confirm pregnancy. Percent (%) of polymorphnuclear
leukocytes (PMNs) taken by CT were expressed by ROC curve (with cut-off point set
at 1%). Out of the total of 51 cows involved in the study, 13.73% true positive and
41.17% true negative cows were found (54.90%). The content of CM sampled with
Metricheck was altered in 11 cows (21.57%), and one of them was pregnant (1.96%).
An average macroscopic CM value for pregnant cows was 0.06±0.24 vs. 0.39±0.79
for non-pregnant (p<0.05). An average value of CM crystallization in pregnant cows
was 2.50±1.04 vs. 1.85±1.15 in non-pregnant (p<0.05). P4 concentration was not
statistically different at AI day, but was statistically significantly higher 17 days later
in pregnant cows (p = 0.002). CT alterations may be a reliable diagnostic tool for
SCE diagnosis, while macroscopic CM alterations may be used for CE detection. P4
determination is beneficial in pregnancy diagnosis while CM crystallization degree is
helpful in determination of best time for AI., Cilj studije bio je procena različitih metoda za dijagnostiku supkliničkog endometritisa
(SKE) i njihove pouzdanosti za predviđanje graviditeta. U studiju je bila uključena 51
postpartalna mlečna krava mešane rase sa patološkim puerperijem. Veštačko osemenjavanje
(VO) rađeno je u spontanom estrusu. Na dan VO uzeti su uzorci endometrijuma
dobijeni cytotape (CT) tehnikom, kao i uzorci cervikalne sluzi (CS) dobijeni
upotrebom Metričeka. Razmaz CS, urađen isti dan, korišten je za ispitivanje stepena
kristalizacije. Uzorci krvi za određivanje koncentracije progesterona (P4) uzeti su na
dan VO i 17 dana kasnije. Krave su pregledane ultrazvukom 35 dana nakon VO radi
potvrđivanja graviditeta. Procenat (%) polimorfonuklearnih leukocita (PMNs) dobijenih CT tehnikom je izražen ROC krivom (granična vrednost je određena na 1%).
Od ukupno 54,90% krava uključenih u studiju, utvrđeno je 13,73% stvarno pozitivnih
i 41,17% stvarno negativnih krava. Izmenjen sadržaj CS uzorkovane Metričekom
utvrđen je kod jedanaest krava (21,57%), ali samo jedna (1,96%) je bila gravidna. Prosečna
makroskopska vrednost CS kod gravidnih krava je bila 0,06±0,24 u odnosu na
0,39±0,79 kod negravidnih (p<0,05). Prosečna vrednost kristalizacije CS kod gravidnih
krava je bila 2,50±1,04 u odnosu na 1,85±1,15 kod negravidnih (p<0,05). Koncentracija
P4 nije se statistički razlikovala na dan VO, ali je bila statistički značajno
veća 17 dana kasnije kod gravidnih životinja (p = 0.002). Promene utvrđene CT mogu
biti indikator SKE, a promene CS dobijene Metričekom mogu biti indikator kliničkog
endometritisa. Određivanje P4 korisno je u dijagnostici graviditeta, a kristalizacija CS
može se koristiti kao pomoćni metod za određivanje najpovoljnijeg vremena za VO.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria-Beograd",
title = "Assessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceive, Procena različitih dijagnostičkih metoda za identifikaciju supkliničkog endometritisa mlečnih krava sa patološkim puerperijumom i njihova pouzdanost u predikciji graviditeta",
volume = "71",
number = "4",
pages = "462-476",
doi = "10.2478/acve-2021-0038"
}
Bajagić, B., Mrkun, J., Kirovski, D., Savić, Đ., Budimir, D., Maletić, J.,& Maletić, M.. (2021). Assessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceive. in Acta veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(4), 462-476.
https://doi.org/10.2478/acve-2021-0038
Bajagić B, Mrkun J, Kirovski D, Savić Đ, Budimir D, Maletić J, Maletić M. Assessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceive. in Acta veterinaria-Beograd. 2021;71(4):462-476.
doi:10.2478/acve-2021-0038 .
Bajagić, Bojana, Mrkun, Janko, Kirovski, Danijela, Savić, Đorđe, Budimir, Draženko, Maletić, Jelena, Maletić, Milan, "Assessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceive" in Acta veterinaria-Beograd, 71, no. 4 (2021):462-476,
https://doi.org/10.2478/acve-2021-0038 . .
2

Retained bovine placenta – various treatments and their effects

Maletić, Milan; Spasojević, Filip; Blagojević, Jovan; Aleksić, Nevenka; Vakanjac, Slobodanka; Maletić, Jelena; Mrkun, Janko

(Fakultet veterinarske medicine, 2021)

TY  - JOUR
AU  - Maletić, Milan
AU  - Spasojević, Filip
AU  - Blagojević, Jovan
AU  - Aleksić, Nevenka
AU  - Vakanjac, Slobodanka
AU  - Maletić, Jelena
AU  - Mrkun, Janko
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2277
AB  - Given the influence of retentio secundinarum (RS) on the reproductive performance of cows, the current work aimed to assess the effect of various approaches to the treatment of retained placenta on the main reproductive parameters, primarily on the interval from parturition to the first service, and the insemination index. The trial involved 120 dairy cows in which the placenta was not expelled within 24 h postpartum. The first group (G1, n=60) contained cows in which the retained placenta was not extirpated, but they were administered antibiotics. The second group (G2, n=60) comprised cows in which the placenta was removed manually, and were also administered antibiotics. At the end of the study, 53 cows remained in G1 and 54 cows in G2. According to medicament therapy, both groups were divided into three sub-groups treated with: procaine benzylpenicillin, chlortetracycline hydrochloride or ceftiofur. Comparison of the performance of G1 and G2 cows showed significantly better fertility parameters occurred in the animals from which the retained placenta was extracted manually. The pregnancy rate in the G2 group was remarkably higher than in the G1 group. The average service interval was significantly lower (p<0.05) in the G2 group compared to the G1 group. The best results were obtained with the i.m. administration of ceftiofur after the removal of the placenta.
AB  - Uzimajući u obzir uticaj RS (retentio secundinarum) na reproduktivne performanse krava,
cilj ovog rada je bio da se proceni efekat različitih pristupa lečenju zadržane posteljice
na glavne reproduktivne parametre, pre svega na interval od porođaja do koncepcije
i indeks osemenjavanja. U ispitivanju je učestvovalo 120 muznih krava kod kojih
nije došlo do izbacivanja posteljice 24 sata nakon porođaja. Prva grupa (G1, n = 60)
uključivala je krave kod kojih zadržana placenta nije manuelno uklanjana, već je terapija
podrazumevala samo aplikaciju antibiotika. Drugu grupu (G2, n = 60) činile su krave
kojima je posteljica odstranjena manuelno i uz to primenjena antibiotska terapija. Do
kraja eksperimenta je u prvoj oglednoj grupi ostalo 53 krave, a u drugoj grupi 54
krave. Prema vrsti medikamentozne terapije, obe grupe su podeljene u tri podgrupe
i lečene su prokain-benzilpenicilinom, hlortetraciklin hidrohloridom i ceftiofurom.
Upoređivanjem reproduktivnih performansi prve i druge grupe krava, primetni su
značajno bolji parametri plodnosti kod životinja kojima je manuelno izvađena zadržana
posteljica i kojima su uz to aplikovani antibiotici. Broj gravidnih životinja u G2 grupi
bio veći u odnosu na G1 grupu. Prosečno trajanje servis perioda bilo je statistički
značajno kraće (p<0,05) u G2 grupi u poređenju sa G1 grupom. Najbolji rezultati
postignuti su i.m. primenom ceftiofura nakon uklanjanja placente.
PB  - Fakultet veterinarske medicine
T2  - Veterinarski Glasnik
T1  - Retained bovine placenta – various treatments and their effects
T1  - Zaostala posteljica kod goveda – različiti pristupi terapiji i njihovi efekti
VL  - 00
SP  - 1
EP  - 10
DO  - 10.2298/VETGL201205003M
ER  - 
@article{
author = "Maletić, Milan and Spasojević, Filip and Blagojević, Jovan and Aleksić, Nevenka and Vakanjac, Slobodanka and Maletić, Jelena and Mrkun, Janko",
year = "2021",
abstract = "Given the influence of retentio secundinarum (RS) on the reproductive performance of cows, the current work aimed to assess the effect of various approaches to the treatment of retained placenta on the main reproductive parameters, primarily on the interval from parturition to the first service, and the insemination index. The trial involved 120 dairy cows in which the placenta was not expelled within 24 h postpartum. The first group (G1, n=60) contained cows in which the retained placenta was not extirpated, but they were administered antibiotics. The second group (G2, n=60) comprised cows in which the placenta was removed manually, and were also administered antibiotics. At the end of the study, 53 cows remained in G1 and 54 cows in G2. According to medicament therapy, both groups were divided into three sub-groups treated with: procaine benzylpenicillin, chlortetracycline hydrochloride or ceftiofur. Comparison of the performance of G1 and G2 cows showed significantly better fertility parameters occurred in the animals from which the retained placenta was extracted manually. The pregnancy rate in the G2 group was remarkably higher than in the G1 group. The average service interval was significantly lower (p<0.05) in the G2 group compared to the G1 group. The best results were obtained with the i.m. administration of ceftiofur after the removal of the placenta., Uzimajući u obzir uticaj RS (retentio secundinarum) na reproduktivne performanse krava,
cilj ovog rada je bio da se proceni efekat različitih pristupa lečenju zadržane posteljice
na glavne reproduktivne parametre, pre svega na interval od porođaja do koncepcije
i indeks osemenjavanja. U ispitivanju je učestvovalo 120 muznih krava kod kojih
nije došlo do izbacivanja posteljice 24 sata nakon porođaja. Prva grupa (G1, n = 60)
uključivala je krave kod kojih zadržana placenta nije manuelno uklanjana, već je terapija
podrazumevala samo aplikaciju antibiotika. Drugu grupu (G2, n = 60) činile su krave
kojima je posteljica odstranjena manuelno i uz to primenjena antibiotska terapija. Do
kraja eksperimenta je u prvoj oglednoj grupi ostalo 53 krave, a u drugoj grupi 54
krave. Prema vrsti medikamentozne terapije, obe grupe su podeljene u tri podgrupe
i lečene su prokain-benzilpenicilinom, hlortetraciklin hidrohloridom i ceftiofurom.
Upoređivanjem reproduktivnih performansi prve i druge grupe krava, primetni su
značajno bolji parametri plodnosti kod životinja kojima je manuelno izvađena zadržana
posteljica i kojima su uz to aplikovani antibiotici. Broj gravidnih životinja u G2 grupi
bio veći u odnosu na G1 grupu. Prosečno trajanje servis perioda bilo je statistički
značajno kraće (p<0,05) u G2 grupi u poređenju sa G1 grupom. Najbolji rezultati
postignuti su i.m. primenom ceftiofura nakon uklanjanja placente.",
publisher = "Fakultet veterinarske medicine",
journal = "Veterinarski Glasnik",
title = "Retained bovine placenta – various treatments and their effects, Zaostala posteljica kod goveda – različiti pristupi terapiji i njihovi efekti",
volume = "00",
pages = "1-10",
doi = "10.2298/VETGL201205003M"
}
Maletić, M., Spasojević, F., Blagojević, J., Aleksić, N., Vakanjac, S., Maletić, J.,& Mrkun, J.. (2021). Retained bovine placenta – various treatments and their effects. in Veterinarski Glasnik
Fakultet veterinarske medicine., 00, 1-10.
https://doi.org/10.2298/VETGL201205003M
Maletić M, Spasojević F, Blagojević J, Aleksić N, Vakanjac S, Maletić J, Mrkun J. Retained bovine placenta – various treatments and their effects. in Veterinarski Glasnik. 2021;00:1-10.
doi:10.2298/VETGL201205003M .
Maletić, Milan, Spasojević, Filip, Blagojević, Jovan, Aleksić, Nevenka, Vakanjac, Slobodanka, Maletić, Jelena, Mrkun, Janko, "Retained bovine placenta – various treatments and their effects" in Veterinarski Glasnik, 00 (2021):1-10,
https://doi.org/10.2298/VETGL201205003M . .
1

Delovanje nespecifičnih činilaca životne sredine na zdravstveno stanje svinja sa komercijalnih farmi (pregled istraživanja)

Bojkovski, Jovan; Prodanov-Radulović, Jasna; Živkov Baloš, Milica; Pavlović, Ivan; Zdravković, Nemanja; Maletić, Jelena; Dobrosavljević, Ivan

(Požarevac : Sitograf RM : Veterinarski specijalistički institut, 2021)

TY  - CONF
AU  - Bojkovski, Jovan
AU  - Prodanov-Radulović, Jasna
AU  - Živkov Baloš, Milica
AU  - Pavlović, Ivan
AU  - Zdravković, Nemanja
AU  - Maletić, Jelena
AU  - Dobrosavljević, Ivan
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3425
AB  - U dužem vremenskom periodu, na velikim komercijlnim farmama svinja praćeno je
prisustvo hemijskih zagađivača životne sredine (teški metali) i njihov uticaj na zdravstveno
stanje životinja.Prisustvo teških metala (Cd, Pb, Hg) u organizmu dovodi često do
ireverzibilnih promena i pojave toksičnosti. Metodom atomske apsorpcione spektrometrije
dokazano je prisustvo sadržaja Pb, Cd, Hg u krvi i ejakulatu nerastova. Rezultati istraživanja
pokazuju da postoji opasnost od kontaminacije stočne hrane teškim metalima i njihovom
deponovanju u organizmu životinja, kao i opasanost po reproduktivnu sposobnost nerastova.
Urađena je i distribucija navedenih metala u krvnoj plazmi zalučene prasadi. Dobijeni rezultati
ukazuju da postoji razlika u resorpciji teških metala (Pb, Cd, Hg) u zavisnosti od starosti
životinje. Naša preporuka za farme industrijskog tipa je da treba da se deluje na smanjenje
rizika od dejstva teških metala, radi na uvođenju višestepenog monitoring kvaliteta sirovina i
gotovih proizvoda, kao i na primeni adekvatnih protektora od toksičnih dejstava ovih agenasa.
PB  - Požarevac : Sitograf RM : Veterinarski specijalistički institut
C3  - 18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021
T1  - Delovanje nespecifičnih činilaca životne sredine na zdravstveno stanje svinja sa komercijalnih farmi (pregled istraživanja)
SP  - 50
EP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3425
ER  - 
@conference{
author = "Bojkovski, Jovan and Prodanov-Radulović, Jasna and Živkov Baloš, Milica and Pavlović, Ivan and Zdravković, Nemanja and Maletić, Jelena and Dobrosavljević, Ivan",
year = "2021",
abstract = "U dužem vremenskom periodu, na velikim komercijlnim farmama svinja praćeno je
prisustvo hemijskih zagađivača životne sredine (teški metali) i njihov uticaj na zdravstveno
stanje životinja.Prisustvo teških metala (Cd, Pb, Hg) u organizmu dovodi često do
ireverzibilnih promena i pojave toksičnosti. Metodom atomske apsorpcione spektrometrije
dokazano je prisustvo sadržaja Pb, Cd, Hg u krvi i ejakulatu nerastova. Rezultati istraživanja
pokazuju da postoji opasnost od kontaminacije stočne hrane teškim metalima i njihovom
deponovanju u organizmu životinja, kao i opasanost po reproduktivnu sposobnost nerastova.
Urađena je i distribucija navedenih metala u krvnoj plazmi zalučene prasadi. Dobijeni rezultati
ukazuju da postoji razlika u resorpciji teških metala (Pb, Cd, Hg) u zavisnosti od starosti
životinje. Naša preporuka za farme industrijskog tipa je da treba da se deluje na smanjenje
rizika od dejstva teških metala, radi na uvođenju višestepenog monitoring kvaliteta sirovina i
gotovih proizvoda, kao i na primeni adekvatnih protektora od toksičnih dejstava ovih agenasa.",
publisher = "Požarevac : Sitograf RM : Veterinarski specijalistički institut",
journal = "18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021",
title = "Delovanje nespecifičnih činilaca životne sredine na zdravstveno stanje svinja sa komercijalnih farmi (pregled istraživanja)",
pages = "50-50",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3425"
}
Bojkovski, J., Prodanov-Radulović, J., Živkov Baloš, M., Pavlović, I., Zdravković, N., Maletić, J.,& Dobrosavljević, I.. (2021). Delovanje nespecifičnih činilaca životne sredine na zdravstveno stanje svinja sa komercijalnih farmi (pregled istraživanja). in 18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021
Požarevac : Sitograf RM : Veterinarski specijalistički institut., 50-50.
https://hdl.handle.net/21.15107/rcub_veterinar_3425
Bojkovski J, Prodanov-Radulović J, Živkov Baloš M, Pavlović I, Zdravković N, Maletić J, Dobrosavljević I. Delovanje nespecifičnih činilaca životne sredine na zdravstveno stanje svinja sa komercijalnih farmi (pregled istraživanja). in 18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021. 2021;:50-50.
https://hdl.handle.net/21.15107/rcub_veterinar_3425 .
Bojkovski, Jovan, Prodanov-Radulović, Jasna, Živkov Baloš, Milica, Pavlović, Ivan, Zdravković, Nemanja, Maletić, Jelena, Dobrosavljević, Ivan, "Delovanje nespecifičnih činilaca životne sredine na zdravstveno stanje svinja sa komercijalnih farmi (pregled istraživanja)" in 18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021 (2021):50-50,
https://hdl.handle.net/21.15107/rcub_veterinar_3425 .

Procena biosigurnosti na farmi visokomlečnih krava

Bojkovski, Jovan; Arsić, Sveta; Vakanjac, Slobodanka; Becskei, Zsolt; Zdravković, Nemanja; Ninković, Milan; Maletić, Jelena; Stanišić, Ljubodrag; Đurić, Miloje; Dobrosavljević, Ivan; Bugarski, Dejan; Stanković, Branislav

(Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila, 2021)

TY  - CONF
AU  - Bojkovski, Jovan
AU  - Arsić, Sveta
AU  - Vakanjac, Slobodanka
AU  - Becskei, Zsolt
AU  - Zdravković, Nemanja
AU  - Ninković, Milan
AU  - Maletić, Jelena
AU  - Stanišić, Ljubodrag
AU  - Đurić, Miloje
AU  - Dobrosavljević, Ivan
AU  - Bugarski, Dejan
AU  - Stanković, Branislav
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3698
AB  - Biosigurnost, dobrobit, dobra proizvođačka praksa i analiza rizika u kritičnim
kontrolim tačkama su veoma značajni elementi u intenzivnoj govedarskoj proizvodnji.
U zaštiti zdravlja goveda i uspehu same proizvodnje, presudna je planska
primena biosigurnosnih mera. Cilj ovog rada je da se iznese procena biosigurnosnih
mera na farmama visokomlečnih krava i da se ukaže na moguće propuste.
Takođe je diskutovano i o mogućim biosigurnosnom rešenjima na farmi krava, kao
i o rutinskim postupcima u zaštiti od potencijalne biološke kontaminacije i preporukama
koje se odnose na sprovođenje biosigurnosnih mera.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - 12. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 7 - 10. oktobar 2021
T1  - Procena biosigurnosti na farmi visokomlečnih krava
SP  - 191
EP  - 199
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3698
ER  - 
@conference{
author = "Bojkovski, Jovan and Arsić, Sveta and Vakanjac, Slobodanka and Becskei, Zsolt and Zdravković, Nemanja and Ninković, Milan and Maletić, Jelena and Stanišić, Ljubodrag and Đurić, Miloje and Dobrosavljević, Ivan and Bugarski, Dejan and Stanković, Branislav",
year = "2021",
abstract = "Biosigurnost, dobrobit, dobra proizvođačka praksa i analiza rizika u kritičnim
kontrolim tačkama su veoma značajni elementi u intenzivnoj govedarskoj proizvodnji.
U zaštiti zdravlja goveda i uspehu same proizvodnje, presudna je planska
primena biosigurnosnih mera. Cilj ovog rada je da se iznese procena biosigurnosnih
mera na farmama visokomlečnih krava i da se ukaže na moguće propuste.
Takođe je diskutovano i o mogućim biosigurnosnom rešenjima na farmi krava, kao
i o rutinskim postupcima u zaštiti od potencijalne biološke kontaminacije i preporukama
koje se odnose na sprovođenje biosigurnosnih mera.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "12. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 7 - 10. oktobar 2021",
title = "Procena biosigurnosti na farmi visokomlečnih krava",
pages = "191-199",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3698"
}
Bojkovski, J., Arsić, S., Vakanjac, S., Becskei, Z., Zdravković, N., Ninković, M., Maletić, J., Stanišić, L., Đurić, M., Dobrosavljević, I., Bugarski, D.,& Stanković, B.. (2021). Procena biosigurnosti na farmi visokomlečnih krava. in 12. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 7 - 10. oktobar 2021
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 191-199.
https://hdl.handle.net/21.15107/rcub_veterinar_3698
Bojkovski J, Arsić S, Vakanjac S, Becskei Z, Zdravković N, Ninković M, Maletić J, Stanišić L, Đurić M, Dobrosavljević I, Bugarski D, Stanković B. Procena biosigurnosti na farmi visokomlečnih krava. in 12. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 7 - 10. oktobar 2021. 2021;:191-199.
https://hdl.handle.net/21.15107/rcub_veterinar_3698 .
Bojkovski, Jovan, Arsić, Sveta, Vakanjac, Slobodanka, Becskei, Zsolt, Zdravković, Nemanja, Ninković, Milan, Maletić, Jelena, Stanišić, Ljubodrag, Đurić, Miloje, Dobrosavljević, Ivan, Bugarski, Dejan, Stanković, Branislav, "Procena biosigurnosti na farmi visokomlečnih krava" in 12. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 7 - 10. oktobar 2021 (2021):191-199,
https://hdl.handle.net/21.15107/rcub_veterinar_3698 .

The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions

Petrović, Slobodan; Maletić, Milan; Lakić, Nada; Aleksić, Nevenka; Maletić, Jelena; Ristanić, Marko; Stanimirović, Zoran

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2020)

TY  - JOUR
AU  - Petrović, Slobodan
AU  - Maletić, Milan
AU  - Lakić, Nada
AU  - Aleksić, Nevenka
AU  - Maletić, Jelena
AU  - Ristanić, Marko
AU  - Stanimirović, Zoran
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2036
AB  - The aim of the current research was to assess the effects of the feed additive made
of lyophilised melon juice (source of superoxide dismutase, SOD) and inactivated live
Saccharomyces cerevisiae (strain R397) cells added to the feed via the product containing
high levels of organically bound selenium (source of selenium-dependant glutathione
peroxidase, Se-GPx) on the semen quality of bulls in heat stress conditions. The 15
bulls chosen for the experiment were assigned to three equal groups (control –group
C; treated group M, given the source of SOD; and group A, treated with the source
of Se-GPx). The research was conducted in summer. The activities of SOD and SeGPx in seminal plasma were determined spectrophotometrically. Computer-assisted
semen analysis was done to determine the sperm counts, motility and velocity. The
temperature and humidity were recorded with a digital data logger.
The average SOD activity in the control bulls was significantly lower than in M
(p<0.001) and A (p<0.001), whilst the average activities in the treated groups did not
differ significantly (p=0.784). Higher average SOD activity compared to the control in
the treated groups showed that both feed additives increased the antioxidative capacity
of the seminal fluid. The average GPx activity in the control was significantly lower
than in groups A (p=0.001) and M (p=0.005), whilst the two treatments did not lead to
significantly different results (p=0.701). The analysis of relations between the activity of
each enzyme and sperm motility and progressive motility in each of the bulls failed to
detect a significant correlation. The analysis of the relation between THI (temperaturehumidity index) and the activity of the antioxidative enzymes revealed that the increase
in THI coincided with the decrease in the SOD activity in the control group, but with
its increase in the treated groups (p>0.05). In all of the three groups with the increase
in THI there was an increase in GPx activity (p>0.05). It can be concluded that in all
of the three groups of bulls there was an increase in the activity of both enzymes in the seminal plasma, but the increase was significantly lower in the control. Thus, the
antioxidative capacity of the seminal plasma of untreated bulls was proven to be lower
in comparison with those of the treated animals.
AB  - Cilj ovog istraživanja bio je ispitivanje efekta liofi lizovanog preparata voćne pulpe dinje (izvor superoksid dismutase – SOD) i inaktivisanih živih ćelija kvasca Sacharomycess cere-visae (soj R397) dodatih u hranu kroz preparat koji sadrži visok nivo organski vezanog selena (izvor selen zavisne glutation-peroksidaze – Se-GPx) na kvalitet semena bikova u uslovima toplotnog stresa. U ogledje bilo uključeno 15 bikova podeljenih u tri grupe (kontrolna – C grupa, ogledna grupa-M kojoj je dodavan izvor SOD – i ogledna grupa –  A  tretirana  izvorom  Se-GPx).  Ogled  je  sproveden  u  letnjem  periodu.  Aktivnosti  SOD i Se-GPx u seminalnoj plazmi određivane su spektofotometrijski. Za utvrđivanje koncentracija spermatozoida, pokretljivosti i brzinskih parametara korišćena je CASA (computer-assisted semen analysis), a za merenje temeperature i vlažnosti koristili smo digi-talni data loger.Prosečna aktivnost SOD u kontrolnoj grupi bikova bila je značajno niža od one u M grupi (p<0,001) i A grupi (p<0,001), dok se prosečne vrednosti u tretiranim grupama nisu međusobno značajno razlikovale (p=0,784). Više prosečne vrednosti SOD su u tretiranim grupama u odnosu na kontrolnu ukazuju da su oba dodatka hrani povećala antioksidativni kapacitet semene plazme. Prosečna aktivnost GPx za kontrolnu grupu je bila značajno niža nego u A (p=0,001) i M grupi bikova (p=0.005), dok se među-sobno M i A grupa nisu značajno razlikovale (p=0,701). Analizom korelacije između aktivnosti svakog od enzima i procenta pokretljivih i progresivno pokretljivih sperma-tozoida kod svakog bika ponaosob nije ustanovljena značajna povezanost. Analizom veze  između  THI  (temperature-humidity  index)  i  aktivnosti  antioksidativnih  enzima  uočeno  je  da  je  sa  porastom  THI  u  kontrolnoj  grupi  došlo  do  smanjenja  aktivnosti  SOD, dok je u tretiranim grupama nastalo povećanje aktivnosti SOD (p>0.05). U sve tri  grupe  bikova  povećanjem  THI  povećavala  se  i  aktivnost  GPx  (p>0.05).  Može  se  zaključiti da je kod sve tri grupe bikova došlo do povećanja aktivnosti oba enzima u semenoj plazmi, ali je povećanje značajno niže u kontrolnoj grupi nego u tretiranim grupama. Time je dokazan manji antioksidativni kapacitet semene plazme netretiranih bikova u odnosu na tretirane.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions
T1  - Efekat dodavanja antioksidanasa u hrani na određene parametre kvaliteta semena bikova u uslovima toplotnog stresa
VL  - 70
IS  - 4
SP  - 453
EP  - 470
DO  - 10.2478/acve-2020-0034
ER  - 
@article{
author = "Petrović, Slobodan and Maletić, Milan and Lakić, Nada and Aleksić, Nevenka and Maletić, Jelena and Ristanić, Marko and Stanimirović, Zoran",
year = "2020",
abstract = "The aim of the current research was to assess the effects of the feed additive made
of lyophilised melon juice (source of superoxide dismutase, SOD) and inactivated live
Saccharomyces cerevisiae (strain R397) cells added to the feed via the product containing
high levels of organically bound selenium (source of selenium-dependant glutathione
peroxidase, Se-GPx) on the semen quality of bulls in heat stress conditions. The 15
bulls chosen for the experiment were assigned to three equal groups (control –group
C; treated group M, given the source of SOD; and group A, treated with the source
of Se-GPx). The research was conducted in summer. The activities of SOD and SeGPx in seminal plasma were determined spectrophotometrically. Computer-assisted
semen analysis was done to determine the sperm counts, motility and velocity. The
temperature and humidity were recorded with a digital data logger.
The average SOD activity in the control bulls was significantly lower than in M
(p<0.001) and A (p<0.001), whilst the average activities in the treated groups did not
differ significantly (p=0.784). Higher average SOD activity compared to the control in
the treated groups showed that both feed additives increased the antioxidative capacity
of the seminal fluid. The average GPx activity in the control was significantly lower
than in groups A (p=0.001) and M (p=0.005), whilst the two treatments did not lead to
significantly different results (p=0.701). The analysis of relations between the activity of
each enzyme and sperm motility and progressive motility in each of the bulls failed to
detect a significant correlation. The analysis of the relation between THI (temperaturehumidity index) and the activity of the antioxidative enzymes revealed that the increase
in THI coincided with the decrease in the SOD activity in the control group, but with
its increase in the treated groups (p>0.05). In all of the three groups with the increase
in THI there was an increase in GPx activity (p>0.05). It can be concluded that in all
of the three groups of bulls there was an increase in the activity of both enzymes in the seminal plasma, but the increase was significantly lower in the control. Thus, the
antioxidative capacity of the seminal plasma of untreated bulls was proven to be lower
in comparison with those of the treated animals., Cilj ovog istraživanja bio je ispitivanje efekta liofi lizovanog preparata voćne pulpe dinje (izvor superoksid dismutase – SOD) i inaktivisanih živih ćelija kvasca Sacharomycess cere-visae (soj R397) dodatih u hranu kroz preparat koji sadrži visok nivo organski vezanog selena (izvor selen zavisne glutation-peroksidaze – Se-GPx) na kvalitet semena bikova u uslovima toplotnog stresa. U ogledje bilo uključeno 15 bikova podeljenih u tri grupe (kontrolna – C grupa, ogledna grupa-M kojoj je dodavan izvor SOD – i ogledna grupa –  A  tretirana  izvorom  Se-GPx).  Ogled  je  sproveden  u  letnjem  periodu.  Aktivnosti  SOD i Se-GPx u seminalnoj plazmi određivane su spektofotometrijski. Za utvrđivanje koncentracija spermatozoida, pokretljivosti i brzinskih parametara korišćena je CASA (computer-assisted semen analysis), a za merenje temeperature i vlažnosti koristili smo digi-talni data loger.Prosečna aktivnost SOD u kontrolnoj grupi bikova bila je značajno niža od one u M grupi (p<0,001) i A grupi (p<0,001), dok se prosečne vrednosti u tretiranim grupama nisu međusobno značajno razlikovale (p=0,784). Više prosečne vrednosti SOD su u tretiranim grupama u odnosu na kontrolnu ukazuju da su oba dodatka hrani povećala antioksidativni kapacitet semene plazme. Prosečna aktivnost GPx za kontrolnu grupu je bila značajno niža nego u A (p=0,001) i M grupi bikova (p=0.005), dok se među-sobno M i A grupa nisu značajno razlikovale (p=0,701). Analizom korelacije između aktivnosti svakog od enzima i procenta pokretljivih i progresivno pokretljivih sperma-tozoida kod svakog bika ponaosob nije ustanovljena značajna povezanost. Analizom veze  između  THI  (temperature-humidity  index)  i  aktivnosti  antioksidativnih  enzima  uočeno  je  da  je  sa  porastom  THI  u  kontrolnoj  grupi  došlo  do  smanjenja  aktivnosti  SOD, dok je u tretiranim grupama nastalo povećanje aktivnosti SOD (p>0.05). U sve tri  grupe  bikova  povećanjem  THI  povećavala  se  i  aktivnost  GPx  (p>0.05).  Može  se  zaključiti da je kod sve tri grupe bikova došlo do povećanja aktivnosti oba enzima u semenoj plazmi, ali je povećanje značajno niže u kontrolnoj grupi nego u tretiranim grupama. Time je dokazan manji antioksidativni kapacitet semene plazme netretiranih bikova u odnosu na tretirane.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions, Efekat dodavanja antioksidanasa u hrani na određene parametre kvaliteta semena bikova u uslovima toplotnog stresa",
volume = "70",
number = "4",
pages = "453-470",
doi = "10.2478/acve-2020-0034"
}
Petrović, S., Maletić, M., Lakić, N., Aleksić, N., Maletić, J., Ristanić, M.,& Stanimirović, Z.. (2020). The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(4), 453-470.
https://doi.org/10.2478/acve-2020-0034
Petrović S, Maletić M, Lakić N, Aleksić N, Maletić J, Ristanić M, Stanimirović Z. The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions. in Acta Veterinaria-Beograd. 2020;70(4):453-470.
doi:10.2478/acve-2020-0034 .
Petrović, Slobodan, Maletić, Milan, Lakić, Nada, Aleksić, Nevenka, Maletić, Jelena, Ristanić, Marko, Stanimirović, Zoran, "The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions" in Acta Veterinaria-Beograd, 70, no. 4 (2020):453-470,
https://doi.org/10.2478/acve-2020-0034 . .
2
2

Najčešći propusti u obezbeđivanju biosigurnosti na farmi svinja

Bojkovski, Jovan; Prodanov-Radulović, Jasna; Vakanjac, Slobodanka; Becskei, Zsolt; Zdravković, Nemanja; Stanišić, Ljubodrag; Đurić, Miloje; Nedić, Svetlana; Dobrosavljević, Ivan; Maletić, Jelena

(Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila, 2020)

TY  - CONF
AU  - Bojkovski, Jovan
AU  - Prodanov-Radulović, Jasna
AU  - Vakanjac, Slobodanka
AU  - Becskei, Zsolt
AU  - Zdravković, Nemanja
AU  - Stanišić, Ljubodrag
AU  - Đurić, Miloje
AU  - Nedić, Svetlana
AU  - Dobrosavljević, Ivan
AU  - Maletić, Jelena
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3535
AB  - Biosigurnost, doborbit, dobra proizvođačka praksa i analiza rizika u kritičnim
kontrolim tačkama su veoma značajni elementi u intenzivnoj proizvodnji svinja.
Planska primena biosigurnosnih mera je presudna u zaštiti zdravlja svinja i uspehu same proizvodnje. Cilj ovog preglednog rada je da prikaže procenu biosigurnosnih mera na komercijalnim farmama svinja i da ukaže na moguće propuste na
farmama. U radu je diskutovano o mogućim rešenjima biosigurnosti na farmi i o
rutinskim postupcima u zaštiti od moguće biološke kontaminacije na komercijalnim farmama kao i odnosu proizvođača prema zaštiti zdravlja svinja.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020
T1  - Najčešći propusti u obezbeđivanju biosigurnosti na farmi svinja
SP  - 185
EP  - 195
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3535
ER  - 
@conference{
author = "Bojkovski, Jovan and Prodanov-Radulović, Jasna and Vakanjac, Slobodanka and Becskei, Zsolt and Zdravković, Nemanja and Stanišić, Ljubodrag and Đurić, Miloje and Nedić, Svetlana and Dobrosavljević, Ivan and Maletić, Jelena",
year = "2020",
abstract = "Biosigurnost, doborbit, dobra proizvođačka praksa i analiza rizika u kritičnim
kontrolim tačkama su veoma značajni elementi u intenzivnoj proizvodnji svinja.
Planska primena biosigurnosnih mera je presudna u zaštiti zdravlja svinja i uspehu same proizvodnje. Cilj ovog preglednog rada je da prikaže procenu biosigurnosnih mera na komercijalnim farmama svinja i da ukaže na moguće propuste na
farmama. U radu je diskutovano o mogućim rešenjima biosigurnosti na farmi i o
rutinskim postupcima u zaštiti od moguće biološke kontaminacije na komercijalnim farmama kao i odnosu proizvođača prema zaštiti zdravlja svinja.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020",
title = "Najčešći propusti u obezbeđivanju biosigurnosti na farmi svinja",
pages = "185-195",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3535"
}
Bojkovski, J., Prodanov-Radulović, J., Vakanjac, S., Becskei, Z., Zdravković, N., Stanišić, L., Đurić, M., Nedić, S., Dobrosavljević, I.,& Maletić, J.. (2020). Najčešći propusti u obezbeđivanju biosigurnosti na farmi svinja. in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 185-195.
https://hdl.handle.net/21.15107/rcub_veterinar_3535
Bojkovski J, Prodanov-Radulović J, Vakanjac S, Becskei Z, Zdravković N, Stanišić L, Đurić M, Nedić S, Dobrosavljević I, Maletić J. Najčešći propusti u obezbeđivanju biosigurnosti na farmi svinja. in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020. 2020;:185-195.
https://hdl.handle.net/21.15107/rcub_veterinar_3535 .
Bojkovski, Jovan, Prodanov-Radulović, Jasna, Vakanjac, Slobodanka, Becskei, Zsolt, Zdravković, Nemanja, Stanišić, Ljubodrag, Đurić, Miloje, Nedić, Svetlana, Dobrosavljević, Ivan, Maletić, Jelena, "Najčešći propusti u obezbeđivanju biosigurnosti na farmi svinja" in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020 (2020):185-195,
https://hdl.handle.net/21.15107/rcub_veterinar_3535 .

The role of veterinarian in the monitoring programs of mastitis control

Maletić, Milan; Magaš, Vladimir; Maletić, Jelena

(Iop Publishing Ltd, Bristol, 2017)

TY  - CONF
AU  - Maletić, Milan
AU  - Magaš, Vladimir
AU  - Maletić, Jelena
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1515
AB  - Mastitis is the most common and the most expensive disease of dairy cows. It is followed by a large number of direct and indirect costs that burden the farms budget and lead to major economic and health losses. The veterinarian at the farm plays a key role in implementing a protocol of biosecurity measures, a protocol of control, therapy, and the suppression of clinical and subclinical mastitis. In order to successfully implement these measures, a good communication between a veterinarian and a farm staff who performs milking procedures is necessary in order to detect and treat all cases of mastitis in time.
PB  - Iop Publishing Ltd, Bristol
C3  - 59th International Meat Industry Conference (MEATCON2017)
T1  - The role of veterinarian in the monitoring programs of mastitis control
VL  - 85
SP  - UNSP 012035
DO  - 10.1088/1755-1315/85/1/012035
ER  - 
@conference{
author = "Maletić, Milan and Magaš, Vladimir and Maletić, Jelena",
year = "2017",
abstract = "Mastitis is the most common and the most expensive disease of dairy cows. It is followed by a large number of direct and indirect costs that burden the farms budget and lead to major economic and health losses. The veterinarian at the farm plays a key role in implementing a protocol of biosecurity measures, a protocol of control, therapy, and the suppression of clinical and subclinical mastitis. In order to successfully implement these measures, a good communication between a veterinarian and a farm staff who performs milking procedures is necessary in order to detect and treat all cases of mastitis in time.",
publisher = "Iop Publishing Ltd, Bristol",
journal = "59th International Meat Industry Conference (MEATCON2017)",
title = "The role of veterinarian in the monitoring programs of mastitis control",
volume = "85",
pages = "UNSP 012035",
doi = "10.1088/1755-1315/85/1/012035"
}
Maletić, M., Magaš, V.,& Maletić, J.. (2017). The role of veterinarian in the monitoring programs of mastitis control. in 59th International Meat Industry Conference (MEATCON2017)
Iop Publishing Ltd, Bristol., 85, UNSP 012035.
https://doi.org/10.1088/1755-1315/85/1/012035
Maletić M, Magaš V, Maletić J. The role of veterinarian in the monitoring programs of mastitis control. in 59th International Meat Industry Conference (MEATCON2017). 2017;85:UNSP 012035.
doi:10.1088/1755-1315/85/1/012035 .
Maletić, Milan, Magaš, Vladimir, Maletić, Jelena, "The role of veterinarian in the monitoring programs of mastitis control" in 59th International Meat Industry Conference (MEATCON2017), 85 (2017):UNSP 012035,
https://doi.org/10.1088/1755-1315/85/1/012035 . .
2
1
1

Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms

Bojkovski, Jovan; Ilić, Vesna; Vasić, A.; Maletić, Jelena; Vakanjac, Slobodanka; Savić, S.; Štukelj, Marina; Zdravković, Nemanja; Tzika, E. D.; Niculae, Mihaela; Milanov, Dubravka

(Hellenic Veterinary Medical Soc, Athens, 2017)

TY  - JOUR
AU  - Bojkovski, Jovan
AU  - Ilić, Vesna
AU  - Vasić, A.
AU  - Maletić, Jelena
AU  - Vakanjac, Slobodanka
AU  - Savić, S.
AU  - Štukelj, Marina
AU  - Zdravković, Nemanja
AU  - Tzika, E. D.
AU  - Niculae, Mihaela
AU  - Milanov, Dubravka
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1525
AB  - The aim of the present study was to determine the porcine circovirus type 2 (PCV2) prevalence in boars in 3 farms in Serbia and their possible relation with alterations of reproductive parameters and blood biochemical parameters [total protein, urea, creatinine and aspartate transaminase levels (AST)]. The prevalence of (PCV2) was evaluated by the presence of specific antibodies. An ELISA assay was used for the detection of PCV2 antibodies in 58 boars' blood sera from Serbian commercial farms, among which 48 were from and reared in the Serbian farms, and 10 were imported from different European Union (EU) countries. Anti PCV2 IgM and/or IgG were detected in sera of 51 (87.93%) boars. Based on the type of antibodies (IgM and IgG PCV2 antibodies), it was concluded that chronic PCV2 infection was the predominant type in tested boars. The imported boars did not have an active infection titer. Biochemical tests in blood didn't show significant differences between PCV2 positive and negative boars. The recorded high prevalence of PCV2 antibodies among boars indirectly proved that PCV2 has been wide spread in the examined farms. Significant difference in the frequency of active, recent and chronic PCV2 infection in boars was found among three farms (p  lt  0.05). Imported boars did not show significant difference in terms of active, recent or chronic PCV2 infection in comparison with boars deriving from Serbian farms (p > 0.05). Among boars of three different breeds (Landrace, Duroc and Yorkshire), a significantly higher prevalence of PCV2 antibodies was revealed in Landrace boars (p=0.003). Reproductive parameters of sows that were inseminated with PCV2 positive or negative boars' semen, showed similar farrowing rate, as well as percentage of live and stillborn piglets, thus indicating that the observed chronic PCV2 infection did not affect significantly the breeding capability of boars.
PB  - Hellenic Veterinary Medical Soc, Athens
T2  - Journal of the Hellenic Veterinary Medical Society
T1  - Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms
VL  - 68
IS  - 3
SP  - 347
EP  - 354
DO  - 10.12681/jhvms.15488
ER  - 
@article{
author = "Bojkovski, Jovan and Ilić, Vesna and Vasić, A. and Maletić, Jelena and Vakanjac, Slobodanka and Savić, S. and Štukelj, Marina and Zdravković, Nemanja and Tzika, E. D. and Niculae, Mihaela and Milanov, Dubravka",
year = "2017",
abstract = "The aim of the present study was to determine the porcine circovirus type 2 (PCV2) prevalence in boars in 3 farms in Serbia and their possible relation with alterations of reproductive parameters and blood biochemical parameters [total protein, urea, creatinine and aspartate transaminase levels (AST)]. The prevalence of (PCV2) was evaluated by the presence of specific antibodies. An ELISA assay was used for the detection of PCV2 antibodies in 58 boars' blood sera from Serbian commercial farms, among which 48 were from and reared in the Serbian farms, and 10 were imported from different European Union (EU) countries. Anti PCV2 IgM and/or IgG were detected in sera of 51 (87.93%) boars. Based on the type of antibodies (IgM and IgG PCV2 antibodies), it was concluded that chronic PCV2 infection was the predominant type in tested boars. The imported boars did not have an active infection titer. Biochemical tests in blood didn't show significant differences between PCV2 positive and negative boars. The recorded high prevalence of PCV2 antibodies among boars indirectly proved that PCV2 has been wide spread in the examined farms. Significant difference in the frequency of active, recent and chronic PCV2 infection in boars was found among three farms (p  lt  0.05). Imported boars did not show significant difference in terms of active, recent or chronic PCV2 infection in comparison with boars deriving from Serbian farms (p > 0.05). Among boars of three different breeds (Landrace, Duroc and Yorkshire), a significantly higher prevalence of PCV2 antibodies was revealed in Landrace boars (p=0.003). Reproductive parameters of sows that were inseminated with PCV2 positive or negative boars' semen, showed similar farrowing rate, as well as percentage of live and stillborn piglets, thus indicating that the observed chronic PCV2 infection did not affect significantly the breeding capability of boars.",
publisher = "Hellenic Veterinary Medical Soc, Athens",
journal = "Journal of the Hellenic Veterinary Medical Society",
title = "Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms",
volume = "68",
number = "3",
pages = "347-354",
doi = "10.12681/jhvms.15488"
}
Bojkovski, J., Ilić, V., Vasić, A., Maletić, J., Vakanjac, S., Savić, S., Štukelj, M., Zdravković, N., Tzika, E. D., Niculae, M.,& Milanov, D.. (2017). Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms. in Journal of the Hellenic Veterinary Medical Society
Hellenic Veterinary Medical Soc, Athens., 68(3), 347-354.
https://doi.org/10.12681/jhvms.15488
Bojkovski J, Ilić V, Vasić A, Maletić J, Vakanjac S, Savić S, Štukelj M, Zdravković N, Tzika ED, Niculae M, Milanov D. Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms. in Journal of the Hellenic Veterinary Medical Society. 2017;68(3):347-354.
doi:10.12681/jhvms.15488 .
Bojkovski, Jovan, Ilić, Vesna, Vasić, A., Maletić, Jelena, Vakanjac, Slobodanka, Savić, S., Štukelj, Marina, Zdravković, Nemanja, Tzika, E. D., Niculae, Mihaela, Milanov, Dubravka, "Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms" in Journal of the Hellenic Veterinary Medical Society, 68, no. 3 (2017):347-354,
https://doi.org/10.12681/jhvms.15488 . .

Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro

Maletić, Jelena; Đelić, Ninoslav; Radaković, Milena; Maletić, Milan; Lakić, Nada; Kukolj, Vladimir; Aleksić, Nevenka; Anđelković, Marko; Stanimirović, Zoran

(Društvo genetičara Srbije, Beograd, 2015)

TY  - JOUR
AU  - Maletić, Jelena
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Maletić, Milan
AU  - Lakić, Nada
AU  - Kukolj, Vladimir
AU  - Aleksić, Nevenka
AU  - Anđelković, Marko
AU  - Stanimirović, Zoran
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1183
AB  - Tulathromycin is a relatively new semi-synthetic macrolide antibiotic, a member of the triamilide group, approved primarly for the treatment of respiratory diseases in cattle and swine. Various genotoxicological studies indicated that tulathromycin is not genotoxic, but no available published data originate from the single-cell gel electrophoresis (Comet) assay. Therefore, the objective of this study was to examine whether it can induce primary DNA damage using in vitro Comet assay in isolated rat lymphocytes. Lymphocytes were treated with a broad spectrum of tulathromycin concentrations (from 1 to 100 mu M) and co-treatment with an antioxidant, catalase (100 IU/mL and 500 IU/mL) was performed. The highest concentrations of tulathromycin (50 and 100 mu M) caused significant increase of DNA damage in rat lymphocytes and catalase did not significantly reduce the DNA-damaging effect of tulathromycin. The results of this study indicate that tulathromycin induces genotoxic effects at high concentrations, that catalase does not exert protective effect in this case.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro
VL  - 47
IS  - 1
SP  - 339
EP  - 348
DO  - 10.2298/GENSR1501339M
ER  - 
@article{
author = "Maletić, Jelena and Đelić, Ninoslav and Radaković, Milena and Maletić, Milan and Lakić, Nada and Kukolj, Vladimir and Aleksić, Nevenka and Anđelković, Marko and Stanimirović, Zoran",
year = "2015",
abstract = "Tulathromycin is a relatively new semi-synthetic macrolide antibiotic, a member of the triamilide group, approved primarly for the treatment of respiratory diseases in cattle and swine. Various genotoxicological studies indicated that tulathromycin is not genotoxic, but no available published data originate from the single-cell gel electrophoresis (Comet) assay. Therefore, the objective of this study was to examine whether it can induce primary DNA damage using in vitro Comet assay in isolated rat lymphocytes. Lymphocytes were treated with a broad spectrum of tulathromycin concentrations (from 1 to 100 mu M) and co-treatment with an antioxidant, catalase (100 IU/mL and 500 IU/mL) was performed. The highest concentrations of tulathromycin (50 and 100 mu M) caused significant increase of DNA damage in rat lymphocytes and catalase did not significantly reduce the DNA-damaging effect of tulathromycin. The results of this study indicate that tulathromycin induces genotoxic effects at high concentrations, that catalase does not exert protective effect in this case.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro",
volume = "47",
number = "1",
pages = "339-348",
doi = "10.2298/GENSR1501339M"
}
Maletić, J., Đelić, N., Radaković, M., Maletić, M., Lakić, N., Kukolj, V., Aleksić, N., Anđelković, M.,& Stanimirović, Z.. (2015). Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro. in Genetika
Društvo genetičara Srbije, Beograd., 47(1), 339-348.
https://doi.org/10.2298/GENSR1501339M
Maletić J, Đelić N, Radaković M, Maletić M, Lakić N, Kukolj V, Aleksić N, Anđelković M, Stanimirović Z. Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro. in Genetika. 2015;47(1):339-348.
doi:10.2298/GENSR1501339M .
Maletić, Jelena, Đelić, Ninoslav, Radaković, Milena, Maletić, Milan, Lakić, Nada, Kukolj, Vladimir, Aleksić, Nevenka, Anđelković, Marko, Stanimirović, Zoran, "Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro" in Genetika, 47, no. 1 (2015):339-348,
https://doi.org/10.2298/GENSR1501339M . .
1
1

Евалуација генотоксичних ефеката и патоморфолошких промена код пацова након апликације тулатромицина

Maletić, Jelena

(Универзитет у Београду, Факултет ветеринарске медицине, 2015)

TY  - THES
AU  - Maletić, Jelena
PY  - 2015
UR  - https://nardus.mpn.gov.rs/handle/123456789/4221
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=2308
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:10213/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=47521807
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3792
AB  - Примена антибиотика у хуманој и ветеринарској медицини праћена је појавомрезистенције бактерија, због чега се непрекидно ради на развоју новихантимикробних лекова. Неопходно је да антибиотици, поред тога што имајуодговарајући спектар дејства, не испољавају значајније токсичне и генотоксичнеефекте.Циљеви овог истраживања били су да се испитају генотоксични ефекатитулатромицина на изолованим лимфоцитима пацова применом in vitro Комет теста,као и проучавање карактера и степена патоморфолошких промена у органима (јетра,бубрег, срце, тестиси и плућа) након супкутане (s.c.) апликације лека пацовима сојаWistar.У комет тесту се користило седам експерименталних концентрацијатулатромицина (у распону од 1-100 μМ), при чему је утврђено да највишеконцентрације (50 μM и 100 μM) тулатромицина доводе до генотоксичних ефекататако што значајно повећавају ниво ДНК оштећења у поређењу са негативномконтролом.Са циљем испитивања способности ћелија да поправе оштећења ДНК насталадејством тулатромицина, изведен је ко-третман са два ДНК инхибитора репарације:хидроксиуреа (HU) и цитозин арабинозид (AraC). Утврђено је да нема значајнеразлике између ефеката третмана са самим тулатромицином и третмана саинхибиторима, што значи да тулатромицин у лимфоцитима пацова не изазиваоштећења ДНК молекула која се могу поправити ћелијским механизмимарепарације.Као додатак, изведен је ко-третман са каталазом са циљем да се утврди да ли јеOH• индуковао оштећења ДНК молекула. Утврђено је да каталаза не редукујеоштећења ДНК настала дејством тулатромицина, што указује на то да каталаза неиспољава протективни ефекат у овом случају...
AB  - The use of antibiotics in human and veterinary medicine is accompanied by theemergence of bacterial resistance, which is constantly working on the development of newantimicrobial drugs. It is essential that antibiotics, in addition to having the appropriaterange of effects, do not show significant toxic and genotoxic effects.The objectives of this study were to investigate the genotoxic effects oftulathromycin in isolated rat lymphocytes using in vitro Comet assay, as well as thecharacter and degree of pathological changes in the organs (liver, kidney, heart, lung, andtestes) after subcutaneous (s.c.) application of the drug to rats Wistar strain.The Comet assay is used seven experimental concentration of tulathromycin (rangingfrom 1-100 μМ), and it was found that the highest concentrations (50 μМ and 100 μМ) oftulathromycin cause genotoxic effects by significantly increasing the level of DNA damagein comparison with the negative control.To test the ability of cells to repair DNA damage caused tulatromycin fact, weperformed co-treatment with two inhibitors of DNA reparation: hydroxyurea (HU) andcytosine arabinoside (AraC). It has been found that there is no significant differencebetween the effects of treatment with a treatment with tulathromycin and inhibitors, whichmeans that in lymphocytes of rats tulatromycin does not cause damage to the DNAmolecules that can be fixed by the cell reparation mechanisms.In addition, we performed co-treatment with catalase, in order to determine whetherthe OH• induced damage to DNA molecules. It was found that catalase did not reduce theeffect of DNA damage caused by tulathromycin, which indicates that catalase does notexhibit a protective effect in this case.In order to determine character and degree of pathological changes in the organs(liver, kidney, heart, lung, and testes), we performed the investigation on rats that receiveddaily tulatromycin s.c. during ten days in different doses: therapeutic dose (2.5 mg / kg) -group E1, three times doses (7.5 mg / kg) - a group of E2, ten (25 mg / kg) - a group E3,and thirty (75 mg / kg) - group E4 times exceeding therapeutic...
PB  - Универзитет у Београду, Факултет ветеринарске медицине
T2  - Универзитет у Београду
T1  - Евалуација генотоксичних ефеката и патоморфолошких промена код пацова након апликације тулатромицина
T1  - Еvaluation of genotoxic effects and pathomorphological in rats after application of tulathromycin
UR  - https://hdl.handle.net/21.15107/rcub_nardus_4221
ER  - 
@phdthesis{
author = "Maletić, Jelena",
year = "2015",
abstract = "Примена антибиотика у хуманој и ветеринарској медицини праћена је појавомрезистенције бактерија, због чега се непрекидно ради на развоју новихантимикробних лекова. Неопходно је да антибиотици, поред тога што имајуодговарајући спектар дејства, не испољавају значајније токсичне и генотоксичнеефекте.Циљеви овог истраживања били су да се испитају генотоксични ефекатитулатромицина на изолованим лимфоцитима пацова применом in vitro Комет теста,као и проучавање карактера и степена патоморфолошких промена у органима (јетра,бубрег, срце, тестиси и плућа) након супкутане (s.c.) апликације лека пацовима сојаWistar.У комет тесту се користило седам експерименталних концентрацијатулатромицина (у распону од 1-100 μМ), при чему је утврђено да највишеконцентрације (50 μM и 100 μM) тулатромицина доводе до генотоксичних ефекататако што значајно повећавају ниво ДНК оштећења у поређењу са негативномконтролом.Са циљем испитивања способности ћелија да поправе оштећења ДНК насталадејством тулатромицина, изведен је ко-третман са два ДНК инхибитора репарације:хидроксиуреа (HU) и цитозин арабинозид (AraC). Утврђено је да нема значајнеразлике између ефеката третмана са самим тулатромицином и третмана саинхибиторима, што значи да тулатромицин у лимфоцитима пацова не изазиваоштећења ДНК молекула која се могу поправити ћелијским механизмимарепарације.Као додатак, изведен је ко-третман са каталазом са циљем да се утврди да ли јеOH• индуковао оштећења ДНК молекула. Утврђено је да каталаза не редукујеоштећења ДНК настала дејством тулатромицина, што указује на то да каталаза неиспољава протективни ефекат у овом случају..., The use of antibiotics in human and veterinary medicine is accompanied by theemergence of bacterial resistance, which is constantly working on the development of newantimicrobial drugs. It is essential that antibiotics, in addition to having the appropriaterange of effects, do not show significant toxic and genotoxic effects.The objectives of this study were to investigate the genotoxic effects oftulathromycin in isolated rat lymphocytes using in vitro Comet assay, as well as thecharacter and degree of pathological changes in the organs (liver, kidney, heart, lung, andtestes) after subcutaneous (s.c.) application of the drug to rats Wistar strain.The Comet assay is used seven experimental concentration of tulathromycin (rangingfrom 1-100 μМ), and it was found that the highest concentrations (50 μМ and 100 μМ) oftulathromycin cause genotoxic effects by significantly increasing the level of DNA damagein comparison with the negative control.To test the ability of cells to repair DNA damage caused tulatromycin fact, weperformed co-treatment with two inhibitors of DNA reparation: hydroxyurea (HU) andcytosine arabinoside (AraC). It has been found that there is no significant differencebetween the effects of treatment with a treatment with tulathromycin and inhibitors, whichmeans that in lymphocytes of rats tulatromycin does not cause damage to the DNAmolecules that can be fixed by the cell reparation mechanisms.In addition, we performed co-treatment with catalase, in order to determine whetherthe OH• induced damage to DNA molecules. It was found that catalase did not reduce theeffect of DNA damage caused by tulathromycin, which indicates that catalase does notexhibit a protective effect in this case.In order to determine character and degree of pathological changes in the organs(liver, kidney, heart, lung, and testes), we performed the investigation on rats that receiveddaily tulatromycin s.c. during ten days in different doses: therapeutic dose (2.5 mg / kg) -group E1, three times doses (7.5 mg / kg) - a group of E2, ten (25 mg / kg) - a group E3,and thirty (75 mg / kg) - group E4 times exceeding therapeutic...",
publisher = "Универзитет у Београду, Факултет ветеринарске медицине",
journal = "Универзитет у Београду",
title = "Евалуација генотоксичних ефеката и патоморфолошких промена код пацова након апликације тулатромицина, Еvaluation of genotoxic effects and pathomorphological in rats after application of tulathromycin",
url = "https://hdl.handle.net/21.15107/rcub_nardus_4221"
}
Maletić, J.. (2015). Евалуација генотоксичних ефеката и патоморфолошких промена код пацова након апликације тулатромицина. in Универзитет у Београду
Универзитет у Београду, Факултет ветеринарске медицине..
https://hdl.handle.net/21.15107/rcub_nardus_4221
Maletić J. Евалуација генотоксичних ефеката и патоморфолошких промена код пацова након апликације тулатромицина. in Универзитет у Београду. 2015;.
https://hdl.handle.net/21.15107/rcub_nardus_4221 .
Maletić, Jelena, "Евалуација генотоксичних ефеката и патоморфолошких промена код пацова након апликације тулатромицина" in Универзитет у Београду (2015),
https://hdl.handle.net/21.15107/rcub_nardus_4221 .