@article{
author = "Jezdimirović, Milanka and Aleksić, Nevenka and Milovanović, Mirjana and Stojanović, Dragica and Jezdimirović, Nemanja",
year = "2013",
abstract = "Ispitivano je moguće hematotoksično, hepatotoksično i nefrotoksičano dejstvo eugenola
kod pacova posle dvonedeljne i četvoronedeljne kontinuirane p.o. primene.
Ogled je izveden na 72 mužjaka pacova soja Wistar podeljenih u šest grupa. Četiri
grupe tretirane su različitim dozama eugenola (10 mg/kg tm/dan, 50 mg/kg tm/dan,
200 mg/kg tm/dan i 400 mg/kg tm/dan). Peta, kontrolna grupa dobijala je vehikulum
(0,5% metil-celuloza, 20% propilen-glikol i voda), a šesta je bila apsolutna, netretirana
kontrola. Eugenol i vehikulum aplikovani su gastričnom sondom, svakodnevno tokom
četiri nedelje u količini od 1 ml/100 g telesne mase pacova. Krv je uzorkovana
kardijalnom punkcijom anestetisanih pacova 14. i 28. dana ogleda u cilju određivanja
hematoloških i biohemijskih parametara krvi: hematokrit, broj eritrocita, leukocita i
trombocita, leukocitna formula, koncentracija hemoglobina, MCV, MCH, koncentracija
proteina, albumina, uree, kreatinina, kao i aktivnost alanin-aminotransferaze (ALT),
alkalne fosfataze (ALP) i kreatin-kinaze (CK). Rezultati su pokazali da eugenol primenjivan tokom dve i četiri nedelje u dozama
od 10, 50, 200 i 400 mg/kg tm/dan ne ispoljava hematotoksično, hepatotoksično niti
nefrotoksično dejstvo. Eugenol davan tokom četiri nedelje ne utiče značajno na broj
eritrocita, koncentraciju hemoglobina, hematokrit, zapreminu eritrocita, broj
leukocita i leukocitnu formulu. Primenjivan tokom dve nedelje, prouzrokuje značajno
povećanje mase hemoglobina po eritrocitu. Ovaj efekat je nespecifičan i ne zavisi niti
doze, niti od dužine tretmana. Doze eugenola od 10 i 200 mg/kg/dan primenjivane
tokom četiri nedelje prouzrokuju značajno smanjenje broja trombocita, dok najviša
ispitivana doza (400 mg/kg/dan) prouzrokuje značajno povećanje, u poređenju sa
brojem trombocita kod pacova tretiranih dozom od 10 i 200 mg/kg/dan. Promene
broja trombocita izazvane eugenolom kvalitativno su različite i ne zavise od doze, niti
od dužine tretmana. Eugenol primenjivan tokom dve i četiri nedelje ne utiče značajno na proteinemiju, albuminemiju, koncentraciju uree i kreatinina, kao i aktivnost ALP u
serumu pacova.
Tri ispitivane doze eugenola (10, 50 i 200 mg/kg/dan) značajno smanjuju, dok
najviša doza povećava aktivnost ALT. Eugenol aplikovan tokom četiri nedelje u dozi
od 200 i 400 mg/kg prouzrokuje statistički značajno smanjenje aktivnosti CK., The possible haematotoxic, hepatotoxic and nephrotoxic effects of eugenol in
rats after two-week and the four-week continuous use were tested. The experiment
was conducted on 72 male Wistar rats divided into six equal groups. Four were
treated with different doses of eugenol (10 mg/kg bw/day, 50 mg/kg/d, 200 mg/kg
bw/d and 400 mg/kg bw/d), the control group received the vehikulum (0.5%
methyl cellulose, 20% propylene glycol and water), and the sixth group was the
absolute, untreated control. Eugenol and the vehikulum were administered with a
gastric probe, on a daily basis, for four weeks at a rate of 1 ml/100 g body weight of
rats. Blood was collected by cardiac puncture of the anesthetised rats on days 14
and 28 in order to assess the haematological and biochemical blood parameters:
haematocrit, red blood cell count, white blood cell and platelet counts, leukocyte
formula, hemoglobin, MCV, MCH, total protein concentration, albumin, urea,
creatinine, and the activity of the alanine aminotransferase (ALT), alkaline
phosphatase (ALP) and creatine kinase (CK).
The results showed that, having been applied for two and four weeks at doses of 10,
50, 200 and 400 mg/kg bw/day, eugenol did not show any haematotoxic, hepatotoxic
or nephrotoxic effects. Eugenol administered for four weeks does not significantly
affect the number of erythrocytes, haemoglobin concentration, haematocrit, red blood cell volume, leukocyte count and leukocyte formula. Applied for two weeks it caused a
significant increase in the mass of haemoglobin in erythrocytes. This effect is not
specific and does not depend on the dose or the treatment duration. Eugenol in doses
of 10 and 200 mg/kg/day applied for four weeks caused a significant reduction in the
number of platelets, whilst the highest dose tested (400 mg/kg/day) caused a
significant increase in comparison to the number of platelets in rats treated with 10
and 200 mg/kg/day. Changes in the number of platelets induced by eugenol
qualitatively different and are not related to dose or length of treatment.
Eugenol applied for two and four weeks does not significantly affect proteinaemia,
albuminaemia, serum urea and creatinine concentrations, and serum ALP
activity in rats. Three assessed doses of eugenol (10, 50 and 200 mg/kg/d)
significantly decreased, whereas the highest dose increased the ALT activity.
However, eugenol applied for four weeks at a dose of 200 and 400 mg/kg/d caused
a statistically significant decrease in CK activity.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Uticaj produžene peroralne primene eugenola na hematološke i neke biohemijske parametre krvi kod pacova, Effect of extended oral administration of eugenol on hematological and some biochemical blood parameters in rats",
volume = "13",
number = "2",
pages = "131-142",
doi = "10.7251/VJRS1302131J"
}
