TY  - JOUR
AU  - Savić, Božidar
AU  - Zdravković, Namanja
AU  - Radanović, Oliver
AU  - Jezdimirović, Nemanja
AU  - Kureljušić, Branislav
AU  - Stevančević, Ognjen
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2512
AB  - Salmonella enterica subspecies enterica serovar Choleraesuis is rarely detected in Europe, but the clinical
disease has been reported in wild boars. We describe here the clinical findings, pathologic changes, and microbiologic features
of swine salmonellosis caused by S. enterica serovar Choleraesuis in weaned piglets in Serbia. In April 2019, on a large
farrow-to-finish pig farm, increased mortality was reported in weaned piglets, marked by lethargy, anorexia, pyrexia, and
respiratory distress. Gross pathology revealed dermal cyanosis, mesenteric lymphadenopathy, splenomegaly, hepatomegaly,
interstitial pneumonia, and colitis. By direct culturing of lung, liver, spleen, and lymph nodes, S. enterica ser. Choleraesuis
variant Kunzendorf was isolated after years of absence of the disease in pig farms in Europe. The source of this salmonellosis
outbreak caused by S. enterica ser. Choleraesuis remains unknown.
PB  - SAGE Publications
T2  - Journal of Veterinary Diagnostic Investigation
T1  - A Salmonella enterica subspecies enterica serovar Choleraesuis outbreak in weaned piglets in Serbia: clinical signs, pathologic changes, and microbiologic features
VL  - 33
IS  - 5
SP  - 993
EP  - 996
DO  - 10.1177/10406387211025507
ER  - 

@article{
author = "Savić, Božidar and Zdravković, Namanja and Radanović, Oliver and Jezdimirović, Nemanja and Kureljušić, Branislav and Stevančević, Ognjen",
year = "2021",
abstract = "Salmonella enterica subspecies enterica serovar Choleraesuis is rarely detected in Europe, but the clinical
disease has been reported in wild boars. We describe here the clinical findings, pathologic changes, and microbiologic features
of swine salmonellosis caused by S. enterica serovar Choleraesuis in weaned piglets in Serbia. In April 2019, on a large
farrow-to-finish pig farm, increased mortality was reported in weaned piglets, marked by lethargy, anorexia, pyrexia, and
respiratory distress. Gross pathology revealed dermal cyanosis, mesenteric lymphadenopathy, splenomegaly, hepatomegaly,
interstitial pneumonia, and colitis. By direct culturing of lung, liver, spleen, and lymph nodes, S. enterica ser. Choleraesuis
variant Kunzendorf was isolated after years of absence of the disease in pig farms in Europe. The source of this salmonellosis
outbreak caused by S. enterica ser. Choleraesuis remains unknown.",
publisher = "SAGE Publications",
journal = "Journal of Veterinary Diagnostic Investigation",
title = "A Salmonella enterica subspecies enterica serovar Choleraesuis outbreak in weaned piglets in Serbia: clinical signs, pathologic changes, and microbiologic features",
volume = "33",
number = "5",
pages = "993-996",
doi = "10.1177/10406387211025507"
}

Savić, B., Zdravković, N., Radanović, O., Jezdimirović, N., Kureljušić, B.,& Stevančević, O.. (2021). A Salmonella enterica subspecies enterica serovar Choleraesuis outbreak in weaned piglets in Serbia: clinical signs, pathologic changes, and microbiologic features. in Journal of Veterinary Diagnostic Investigation
SAGE Publications., 33(5), 993-996.
https://doi.org/10.1177/10406387211025507

Savić B, Zdravković N, Radanović O, Jezdimirović N, Kureljušić B, Stevančević O. A Salmonella enterica subspecies enterica serovar Choleraesuis outbreak in weaned piglets in Serbia: clinical signs, pathologic changes, and microbiologic features. in Journal of Veterinary Diagnostic Investigation. 2021;33(5):993-996.
doi:10.1177/10406387211025507 .

Savić, Božidar, Zdravković, Namanja, Radanović, Oliver, Jezdimirović, Nemanja, Kureljušić, Branislav, Stevančević, Ognjen, "A Salmonella enterica subspecies enterica serovar Choleraesuis outbreak in weaned piglets in Serbia: clinical signs, pathologic changes, and microbiologic features" in Journal of Veterinary Diagnostic Investigation, 33, no. 5 (2021):993-996,
https://doi.org/10.1177/10406387211025507 . .

TY  - JOUR
AU  - Maksimović Zorić, Jelena
AU  - Milićević, Vesna
AU  - Stevančević, Ognjen
AU  - Chiapponi, Chiara
AU  - Potkonjak, Aleksandar
AU  - Stojanac, Nenad
AU  - Kureljušić, Branislav
AU  - Veljović, Lubiša
AU  - Radosavljević, Vladimir
AU  - Savić, Božidar
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1987
AB  - Pigs are very important for the epidemiology of influenza A viruses, being commonly infected with the lineages of most adapted H1N1, H3N2, H1N2 swine subtypes. Epidemiological complexity of swine influenza is increasing by a periodic spillover of human or avian viruses in the pig population when genetic shifts can occur. The objectives of this research were to determine the presence of the influenza A virus in nasal and tracheobronchial swabs and lung tissue samples of ill and dead pigs on commercial farms, to determine circulating subtypes and characterize them through the phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes. A total of 255 samples collected from 13 farms were analyzed by means of real-time RT-PCR. The genome of influenza A virus was detected in 24 samples, which represented a 61.5% prevalence at the farms level (influenza A virus was confirmed in 8 out of 13 farms included in this study). Based on HA and NA gene sequences of 8 viruses, the circulation of H1N1 and H3N2 subtypes of influenza A viruses were determined. In addition, one farm exhibited a time separated circulation of H1N1 and H3N2 virus subtypes. Using Influenza Research Database, our viruses of the H1 subtype were classified into 1C.2.1 and 1A.3.3.2. clade. Based on the nucleotide sequences of HA genes, three viruses of the H1N1 subtype belong to the H1N1pdm09 lineage, and the other four to Eurasian “avian-like” H1avN1 lineage; while based on NA genes sequences, these seven viruses belong to Eurasian “avian-like” H1avN1 lineage. Both HA and NA genes of the virus of the H3N2 subtype belonged to the A/swine/ Gent/1/1984-like H3N2 lineage.
AB  - Svinje su veoma važne u epidemiologiji infl uenca A virusa, jer je većina zapata širom sveta zaražena nekim od tri podtipa (H1N1, H1N2 ili H3N2). Pored toga, one su prijemčive i za ljudske i ptičije infl eunca A viruse, usled čega u njihovom organizmu može doći do genetskog reasortiranja i stvaranja genotipski i fenotipski novih virusa. Cilj ovog istraživanja je bio da se analizom nosnih i traeo-bronhijalnih briseva i pluća poreklom od bolesnih i ugilnulih svinja ispita prisustvo infl uenca A virusa, da se odrede cirkulišući podtipovi i fi logenetski okarakterišu kroz analizu sekvenci HA i NA gena. Tokom ovog istraživanja sa 13 farmi je sakupljeno i metodom realtime RT-PCR pregledano je 255 uzoraka. Genom infl uenca A virusa je utvrđen u 24 uzorka. Prevalencija virusa na nivou farmi iznosila je 61.5%. Kompletno je sekvenciran genom 8 virusa koji su poticali sa sedam farmi. Na osnovu sekvenci HA i NA gena potvrđena je cirkulacija H1N1 i H3N2 podtipova. Na jednoj farmi ustanovlejna je cirkulacija oba podtipa, ali u različitim periodima ispitivanja. Na osnovu sekvence HA gena, sedam virusa H1 podtipa su grupisani u 1C.2.1 i 1A.3.3.2 genske grupe i H1N1pdm09 i evroazijsku “avian-like” H1avN1 linije. Na osnovu sekvence neuraminidaza gena ovih sedam H1N1 virusa su grupisani u evroazijsku “avian-like” H1avN1 liniju. Virus H3N2 podtipa na osnovu sekvenci HA i NA gena pripada liniji A/Swine/ Gent/1/1984 - “like” liniji.
PB  - Sciendo
T2  - Acta Veterinaria-Beograd
T1  - Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018
T1  - Filogenetska analiza HA i Na gena virusa influence svinja u Srbiji od 2016 do 2018 godine
VL  - 70
IS  - 1
SP  - 110
EP  - 125
DO  - 10.2478/acve-2020-0008
ER  - 

@article{
author = "Maksimović Zorić, Jelena and Milićević, Vesna and Stevančević, Ognjen and Chiapponi, Chiara and Potkonjak, Aleksandar and Stojanac, Nenad and Kureljušić, Branislav and Veljović, Lubiša and Radosavljević, Vladimir and Savić, Božidar",
year = "2020",
abstract = "Pigs are very important for the epidemiology of influenza A viruses, being commonly infected with the lineages of most adapted H1N1, H3N2, H1N2 swine subtypes. Epidemiological complexity of swine influenza is increasing by a periodic spillover of human or avian viruses in the pig population when genetic shifts can occur. The objectives of this research were to determine the presence of the influenza A virus in nasal and tracheobronchial swabs and lung tissue samples of ill and dead pigs on commercial farms, to determine circulating subtypes and characterize them through the phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes. A total of 255 samples collected from 13 farms were analyzed by means of real-time RT-PCR. The genome of influenza A virus was detected in 24 samples, which represented a 61.5% prevalence at the farms level (influenza A virus was confirmed in 8 out of 13 farms included in this study). Based on HA and NA gene sequences of 8 viruses, the circulation of H1N1 and H3N2 subtypes of influenza A viruses were determined. In addition, one farm exhibited a time separated circulation of H1N1 and H3N2 virus subtypes. Using Influenza Research Database, our viruses of the H1 subtype were classified into 1C.2.1 and 1A.3.3.2. clade. Based on the nucleotide sequences of HA genes, three viruses of the H1N1 subtype belong to the H1N1pdm09 lineage, and the other four to Eurasian “avian-like” H1avN1 lineage; while based on NA genes sequences, these seven viruses belong to Eurasian “avian-like” H1avN1 lineage. Both HA and NA genes of the virus of the H3N2 subtype belonged to the A/swine/ Gent/1/1984-like H3N2 lineage., Svinje su veoma važne u epidemiologiji infl uenca A virusa, jer je većina zapata širom sveta zaražena nekim od tri podtipa (H1N1, H1N2 ili H3N2). Pored toga, one su prijemčive i za ljudske i ptičije infl eunca A viruse, usled čega u njihovom organizmu može doći do genetskog reasortiranja i stvaranja genotipski i fenotipski novih virusa. Cilj ovog istraživanja je bio da se analizom nosnih i traeo-bronhijalnih briseva i pluća poreklom od bolesnih i ugilnulih svinja ispita prisustvo infl uenca A virusa, da se odrede cirkulišući podtipovi i fi logenetski okarakterišu kroz analizu sekvenci HA i NA gena. Tokom ovog istraživanja sa 13 farmi je sakupljeno i metodom realtime RT-PCR pregledano je 255 uzoraka. Genom infl uenca A virusa je utvrđen u 24 uzorka. Prevalencija virusa na nivou farmi iznosila je 61.5%. Kompletno je sekvenciran genom 8 virusa koji su poticali sa sedam farmi. Na osnovu sekvenci HA i NA gena potvrđena je cirkulacija H1N1 i H3N2 podtipova. Na jednoj farmi ustanovlejna je cirkulacija oba podtipa, ali u različitim periodima ispitivanja. Na osnovu sekvence HA gena, sedam virusa H1 podtipa su grupisani u 1C.2.1 i 1A.3.3.2 genske grupe i H1N1pdm09 i evroazijsku “avian-like” H1avN1 linije. Na osnovu sekvence neuraminidaza gena ovih sedam H1N1 virusa su grupisani u evroazijsku “avian-like” H1avN1 liniju. Virus H3N2 podtipa na osnovu sekvenci HA i NA gena pripada liniji A/Swine/ Gent/1/1984 - “like” liniji.",
publisher = "Sciendo",
journal = "Acta Veterinaria-Beograd",
title = "Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018, Filogenetska analiza HA i Na gena virusa influence svinja u Srbiji od 2016 do 2018 godine",
volume = "70",
number = "1",
pages = "110-125",
doi = "10.2478/acve-2020-0008"
}

Maksimović Zorić, J., Milićević, V., Stevančević, O., Chiapponi, C., Potkonjak, A., Stojanac, N., Kureljušić, B., Veljović, L., Radosavljević, V.,& Savić, B.. (2020). Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018. in Acta Veterinaria-Beograd
Sciendo., 70(1), 110-125.
https://doi.org/10.2478/acve-2020-0008

Maksimović Zorić J, Milićević V, Stevančević O, Chiapponi C, Potkonjak A, Stojanac N, Kureljušić B, Veljović L, Radosavljević V, Savić B. Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018. in Acta Veterinaria-Beograd. 2020;70(1):110-125.
doi:10.2478/acve-2020-0008 .

Maksimović Zorić, Jelena, Milićević, Vesna, Stevančević, Ognjen, Chiapponi, Chiara, Potkonjak, Aleksandar, Stojanac, Nenad, Kureljušić, Branislav, Veljović, Lubiša, Radosavljević, Vladimir, Savić, Božidar, "Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018" in Acta Veterinaria-Beograd, 70, no. 1 (2020):110-125,
https://doi.org/10.2478/acve-2020-0008 . .

TY  - JOUR
AU  - Savić, Božidar
AU  - Milićević, Vesna
AU  - Radanović, Oliver
AU  - Zdravković, Nemanja
AU  - Stevančević, Ognjen
AU  - Kureljušić, Branislav
AU  - Nešić, Ksenija
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1990
AB  - Backgroud: The presence of PCV3 genome has been detected in pigs affected by different clinical and pathological conditions as well as in healthy animals. Its presence has been reported in many countries of North and South America, Asia and Europe. However, there is no evidence of the presence and genetic characteristics of PCV3 in many European countries and especially the countries of the Balkan Peninsula. Purpose: The major objective of this study was to investigate the presence and obtain further genetic characterization of PCV-3 in the pig populations in Serbia. Methods: To demonstrate the presence of PCV-3 DNA a conventional PCR assay was performed. The samples where no PCR product was observed (n=32), were further tested with a real-time PCR assay. The six PCR samples that were strongly positive for PCV-3 were subjected to amplification and sequencing of their entire cap genes and complete viral genome. Results: We report on the first identification, genetic diversity and potential association in pathogenesis of some systemic and respiratory swine diseases of PCV-3 in Serbia. Conclusion: The results imply that PCV-3 circulates widely in the pig population and has a high similarity with previously reported isolates. Detected PCV-3 can be associated with some swine systemic and respiratory diseases but these associations are strongly influenced by the clinical or pathological condition of the animals. Our findings demonstrate that there are certain PCV-3 loads in pigs suffering from active PRRSV infection, Glässer’s disease, APP pleuropneumonia, pneumonic pasteurellosis and PRDC; however, the significance of this viral load, as well as the mechanism by which PCV-3 may act as a secondary agent in aggravating the severity during co-infections of these pathogens, requires further research.
PB  - Springer
T2  - Archives of Virology
T1  - Identification and genetic characterization of porcine circovirus 3 on pig farms in Serbia
VL  - 165
IS  - 1
SP  - 193
EP  - 199
DO  - 10.1007/s00705-019-04455-y
ER  - 

@article{
author = "Savić, Božidar and Milićević, Vesna and Radanović, Oliver and Zdravković, Nemanja and Stevančević, Ognjen and Kureljušić, Branislav and Nešić, Ksenija",
year = "2020",
abstract = "Backgroud: The presence of PCV3 genome has been detected in pigs affected by different clinical and pathological conditions as well as in healthy animals. Its presence has been reported in many countries of North and South America, Asia and Europe. However, there is no evidence of the presence and genetic characteristics of PCV3 in many European countries and especially the countries of the Balkan Peninsula. Purpose: The major objective of this study was to investigate the presence and obtain further genetic characterization of PCV-3 in the pig populations in Serbia. Methods: To demonstrate the presence of PCV-3 DNA a conventional PCR assay was performed. The samples where no PCR product was observed (n=32), were further tested with a real-time PCR assay. The six PCR samples that were strongly positive for PCV-3 were subjected to amplification and sequencing of their entire cap genes and complete viral genome. Results: We report on the first identification, genetic diversity and potential association in pathogenesis of some systemic and respiratory swine diseases of PCV-3 in Serbia. Conclusion: The results imply that PCV-3 circulates widely in the pig population and has a high similarity with previously reported isolates. Detected PCV-3 can be associated with some swine systemic and respiratory diseases but these associations are strongly influenced by the clinical or pathological condition of the animals. Our findings demonstrate that there are certain PCV-3 loads in pigs suffering from active PRRSV infection, Glässer’s disease, APP pleuropneumonia, pneumonic pasteurellosis and PRDC; however, the significance of this viral load, as well as the mechanism by which PCV-3 may act as a secondary agent in aggravating the severity during co-infections of these pathogens, requires further research.",
publisher = "Springer",
journal = "Archives of Virology",
title = "Identification and genetic characterization of porcine circovirus 3 on pig farms in Serbia",
volume = "165",
number = "1",
pages = "193-199",
doi = "10.1007/s00705-019-04455-y"
}

Savić, B., Milićević, V., Radanović, O., Zdravković, N., Stevančević, O., Kureljušić, B.,& Nešić, K.. (2020). Identification and genetic characterization of porcine circovirus 3 on pig farms in Serbia. in Archives of Virology
Springer., 165(1), 193-199.
https://doi.org/10.1007/s00705-019-04455-y

Savić B, Milićević V, Radanović O, Zdravković N, Stevančević O, Kureljušić B, Nešić K. Identification and genetic characterization of porcine circovirus 3 on pig farms in Serbia. in Archives of Virology. 2020;165(1):193-199.
doi:10.1007/s00705-019-04455-y .

Savić, Božidar, Milićević, Vesna, Radanović, Oliver, Zdravković, Nemanja, Stevančević, Ognjen, Kureljušić, Branislav, Nešić, Ksenija, "Identification and genetic characterization of porcine circovirus 3 on pig farms in Serbia" in Archives of Virology, 165, no. 1 (2020):193-199,
https://doi.org/10.1007/s00705-019-04455-y . .

TY  - JOUR
AU  - Tomić, Zdravko
AU  - Stojanac, Nenad
AU  - Cincović, Marko
AU  - Novakov, Nikolina
AU  - Kovačević, Zorana
AU  - Stevancević, Ognjen
AU  - Aleksić, Jelena
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1584
AB  - Determining the post-mortem interval (PMI) is often a very demanding and delicate job which requires a good knowledge of postmortem changes. In this study, 20 domestic pig carcasses (Sus scrofa) whose death occurred within 8 h before the start of the study were simultaneously laid at the same geographical location, but in different environments (on the ground surface - S; buried in the ground - G; placed in a crate and buried in the ground - C; submerged in water - W; and hanging in the air - A). One carcass from each group was sampled on days 14, 28, 120 and 190 from the beginning of the experiment, and on that occasion, a detailed analysis of postmortem changes and an autopsy was carried out. The difference in the rate of decomposition among groups was statistically significant. The fastest decomposition occurred in carcasses placed in a crate and buried, because during the winter period the temperature in the air was below 0 degrees C. At that time, the decomposition process and the insect activity were slowed or stopped on carcasses in groups S, A, W, and G to some extent, while the ground and wooden crate were good thermal insulators for group C carcasses arid provided better conditions for insect activity.
PB  - Kafkas Univ, Veteriner Fakultesi Dergisi, Kars
T2  - Kafkas Üniversitesi Veteriner Fakültesi Dergisi
T1  - The Influence of Carcass Microlocation on the Speed of Postmortem Changes and Carcass Decomposition
VL  - 24
IS  - 5
SP  - 655
EP  - 662
DO  - 10.9775/kvfd.2018.19670
ER  - 

@article{
author = "Tomić, Zdravko and Stojanac, Nenad and Cincović, Marko and Novakov, Nikolina and Kovačević, Zorana and Stevancević, Ognjen and Aleksić, Jelena",
year = "2018",
abstract = "Determining the post-mortem interval (PMI) is often a very demanding and delicate job which requires a good knowledge of postmortem changes. In this study, 20 domestic pig carcasses (Sus scrofa) whose death occurred within 8 h before the start of the study were simultaneously laid at the same geographical location, but in different environments (on the ground surface - S; buried in the ground - G; placed in a crate and buried in the ground - C; submerged in water - W; and hanging in the air - A). One carcass from each group was sampled on days 14, 28, 120 and 190 from the beginning of the experiment, and on that occasion, a detailed analysis of postmortem changes and an autopsy was carried out. The difference in the rate of decomposition among groups was statistically significant. The fastest decomposition occurred in carcasses placed in a crate and buried, because during the winter period the temperature in the air was below 0 degrees C. At that time, the decomposition process and the insect activity were slowed or stopped on carcasses in groups S, A, W, and G to some extent, while the ground and wooden crate were good thermal insulators for group C carcasses arid provided better conditions for insect activity.",
publisher = "Kafkas Univ, Veteriner Fakultesi Dergisi, Kars",
journal = "Kafkas Üniversitesi Veteriner Fakültesi Dergisi",
title = "The Influence of Carcass Microlocation on the Speed of Postmortem Changes and Carcass Decomposition",
volume = "24",
number = "5",
pages = "655-662",
doi = "10.9775/kvfd.2018.19670"
}

Tomić, Z., Stojanac, N., Cincović, M., Novakov, N., Kovačević, Z., Stevancević, O.,& Aleksić, J.. (2018). The Influence of Carcass Microlocation on the Speed of Postmortem Changes and Carcass Decomposition. in Kafkas Üniversitesi Veteriner Fakültesi Dergisi
Kafkas Univ, Veteriner Fakultesi Dergisi, Kars., 24(5), 655-662.
https://doi.org/10.9775/kvfd.2018.19670

Tomić Z, Stojanac N, Cincović M, Novakov N, Kovačević Z, Stevancević O, Aleksić J. The Influence of Carcass Microlocation on the Speed of Postmortem Changes and Carcass Decomposition. in Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2018;24(5):655-662.
doi:10.9775/kvfd.2018.19670 .

Tomić, Zdravko, Stojanac, Nenad, Cincović, Marko, Novakov, Nikolina, Kovačević, Zorana, Stevancević, Ognjen, Aleksić, Jelena, "The Influence of Carcass Microlocation on the Speed of Postmortem Changes and Carcass Decomposition" in Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 24, no. 5 (2018):655-662,
https://doi.org/10.9775/kvfd.2018.19670 . .

TY  - JOUR
AU  - Potkonjak, Aleksandar
AU  - Vračar, Vuk
AU  - Stančić, Ivan
AU  - Spasojević-Kosić, Ljubica
AU  - Bacić, Dragan
AU  - Cincović, Marko
AU  - Toholj, Bojan
AU  - Stevančević, Ognjen
AU  - Ristić, Zoran
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1139
AB  - The aim of this study was to determine the prevalence of coinfection with pathogens Bartonella henselae, feline immunodeficiency virus, and feline leukemia virus in stray cats from the area of Novi Sad and Belgrade, Serbia. Each of 60 individual cats was clinically examined and the blood sampled. Therewithal an epidemiological survey was made. Blood sera were separated by centrifugation and serologically tested in order to determine the presence of Bartonella henselae specific antibodies (by direct immunofluorescence assay), feline immunodeficiency virus specific antibodies (by rapid test SNAP Combo) and feline leukemia virus antigens (by rapid test SNAP Combo). Of the 60 cat sera, serologically examined using IFA test, 33 (55%) were positive for the presence of IgG specific to B. henselae antigens. A total of 13 (27%) of the 60 tested cat sera were positive for the presence of specific antibodies to FIV antigens. None of the 60 tested cat sera were positive for the presence of FeLV antigen. Of the 33 cat sera which contained IgG antibodies to B. henselae, 6 cat sera also gave a positive reaction to the presence of specific IgG antibodies to FIV; this was a coinfection seroprevalence of 10% in the total population of studied cats. The results obtained in this study indicate the presence of B. henselae and FIV coinfection in cats from Serbia, without FeLV positive cats. An increase in the manifestations of clinical symptoms in cats in which the serological tests determined coinfection with B. henselae and FIV is evident compared to those seropositive only to B. henselae.
AB  - Cilj ovog istraživanja je da se utvrdi koinfekcija uzročnicima Bartonella henselae, virus imunodeficijencije mačaka i virus leukemije mačaka, kod uličnih mačaka s područja Novog Sada i Beograda, Srbija. Od svake od ukupno 60 jedinki uključenih u istraživanje uzeti su uzorci pune venske krvi. Paralelno s uzimanjem uzoraka obavljen je klinički pregled mačaka i sačinjena je epidemiološka anketa. Krvni serumi izdvojeni su centrifugiranjem i serološki su testirani u cilju utvrđivanja prisustva specifičnih antitela na uzročnike Bartonella henselae (testom indirektne imunofl uorescencije-IFA) i virus mačije imunodeficijencije (FIV) (brzim testom SNAP Combo), kao i antigena virusa leukemije mačaka (FeLV) (brzim testom SNAP Combo). Od 60 krvnih seruma mačaka, serološki pregledanih korišćenjem IFA testa, 33 (55%) je bilo pozitivno na prisustvo specifičnih IgG antitela na antigene uzročnika Bartonella henselae. Od 60 ispitivanih uzoraka seruma mačaka, 13 je dalo pozitivnu reakciju na prisustvo specifičnih antitela na antigene FIV. Niti u jednom od 60 ispitivanih uzoraka seruma nije utvrđeno prisustvo antigena FeLV. Od 33 krvna seruma mačaka, u kojima je ustanovljeno prisustvo specifičnih IgG antitela na B. henselae, 6 krvnih seruma dalo je pozitivnu reakciju i na prisustvo specifičnih IgG antitela na uzročnika virusne imunodeficijencije mačaka, što predstavlja seroprevalenciju koinfekcije od 10% u ukupnoj populaciji ispitivanih mačaka. Rezultati dobijeni u ovom istraživanju ukazuju na prisustvo koinfekcije uzročnicima Bartonella henselae i FIV kod mačaka u Srbiji, bez FeLV pozitivnih jedinki. Evidentan je porast manifestacije kliničkih simptoma kod mačaka kod kojih je primenom seroloških testova utvrđena koinfekcija izazvana uzročnicima B. henselae i FIV, u poređenju sa mačkama kod kojih je ustanovljena infekcija izazvana samo uzročnikom B. henselae.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Occurrence of Bartonella henselae, FeLV and FIV infection in 60 stray cats from Serbia
T1  - Pojava Bartonella henselae, FeLV i FIV infekcije kod 60 uličnih mačaka u Srbiji
VL  - 64
IS  - 3
SP  - 378
EP  - 385
DO  - 10.2478/acve-2014-0036
ER  - 

@article{
author = "Potkonjak, Aleksandar and Vračar, Vuk and Stančić, Ivan and Spasojević-Kosić, Ljubica and Bacić, Dragan and Cincović, Marko and Toholj, Bojan and Stevančević, Ognjen and Ristić, Zoran",
year = "2014",
abstract = "The aim of this study was to determine the prevalence of coinfection with pathogens Bartonella henselae, feline immunodeficiency virus, and feline leukemia virus in stray cats from the area of Novi Sad and Belgrade, Serbia. Each of 60 individual cats was clinically examined and the blood sampled. Therewithal an epidemiological survey was made. Blood sera were separated by centrifugation and serologically tested in order to determine the presence of Bartonella henselae specific antibodies (by direct immunofluorescence assay), feline immunodeficiency virus specific antibodies (by rapid test SNAP Combo) and feline leukemia virus antigens (by rapid test SNAP Combo). Of the 60 cat sera, serologically examined using IFA test, 33 (55%) were positive for the presence of IgG specific to B. henselae antigens. A total of 13 (27%) of the 60 tested cat sera were positive for the presence of specific antibodies to FIV antigens. None of the 60 tested cat sera were positive for the presence of FeLV antigen. Of the 33 cat sera which contained IgG antibodies to B. henselae, 6 cat sera also gave a positive reaction to the presence of specific IgG antibodies to FIV; this was a coinfection seroprevalence of 10% in the total population of studied cats. The results obtained in this study indicate the presence of B. henselae and FIV coinfection in cats from Serbia, without FeLV positive cats. An increase in the manifestations of clinical symptoms in cats in which the serological tests determined coinfection with B. henselae and FIV is evident compared to those seropositive only to B. henselae., Cilj ovog istraživanja je da se utvrdi koinfekcija uzročnicima Bartonella henselae, virus imunodeficijencije mačaka i virus leukemije mačaka, kod uličnih mačaka s područja Novog Sada i Beograda, Srbija. Od svake od ukupno 60 jedinki uključenih u istraživanje uzeti su uzorci pune venske krvi. Paralelno s uzimanjem uzoraka obavljen je klinički pregled mačaka i sačinjena je epidemiološka anketa. Krvni serumi izdvojeni su centrifugiranjem i serološki su testirani u cilju utvrđivanja prisustva specifičnih antitela na uzročnike Bartonella henselae (testom indirektne imunofl uorescencije-IFA) i virus mačije imunodeficijencije (FIV) (brzim testom SNAP Combo), kao i antigena virusa leukemije mačaka (FeLV) (brzim testom SNAP Combo). Od 60 krvnih seruma mačaka, serološki pregledanih korišćenjem IFA testa, 33 (55%) je bilo pozitivno na prisustvo specifičnih IgG antitela na antigene uzročnika Bartonella henselae. Od 60 ispitivanih uzoraka seruma mačaka, 13 je dalo pozitivnu reakciju na prisustvo specifičnih antitela na antigene FIV. Niti u jednom od 60 ispitivanih uzoraka seruma nije utvrđeno prisustvo antigena FeLV. Od 33 krvna seruma mačaka, u kojima je ustanovljeno prisustvo specifičnih IgG antitela na B. henselae, 6 krvnih seruma dalo je pozitivnu reakciju i na prisustvo specifičnih IgG antitela na uzročnika virusne imunodeficijencije mačaka, što predstavlja seroprevalenciju koinfekcije od 10% u ukupnoj populaciji ispitivanih mačaka. Rezultati dobijeni u ovom istraživanju ukazuju na prisustvo koinfekcije uzročnicima Bartonella henselae i FIV kod mačaka u Srbiji, bez FeLV pozitivnih jedinki. Evidentan je porast manifestacije kliničkih simptoma kod mačaka kod kojih je primenom seroloških testova utvrđena koinfekcija izazvana uzročnicima B. henselae i FIV, u poređenju sa mačkama kod kojih je ustanovljena infekcija izazvana samo uzročnikom B. henselae.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Occurrence of Bartonella henselae, FeLV and FIV infection in 60 stray cats from Serbia, Pojava Bartonella henselae, FeLV i FIV infekcije kod 60 uličnih mačaka u Srbiji",
volume = "64",
number = "3",
pages = "378-385",
doi = "10.2478/acve-2014-0036"
}

Potkonjak, A., Vračar, V., Stančić, I., Spasojević-Kosić, L., Bacić, D., Cincović, M., Toholj, B., Stevančević, O.,& Ristić, Z.. (2014). Occurrence of Bartonella henselae, FeLV and FIV infection in 60 stray cats from Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(3), 378-385.
https://doi.org/10.2478/acve-2014-0036

Potkonjak A, Vračar V, Stančić I, Spasojević-Kosić L, Bacić D, Cincović M, Toholj B, Stevančević O, Ristić Z. Occurrence of Bartonella henselae, FeLV and FIV infection in 60 stray cats from Serbia. in Acta Veterinaria-Beograd. 2014;64(3):378-385.
doi:10.2478/acve-2014-0036 .

Potkonjak, Aleksandar, Vračar, Vuk, Stančić, Ivan, Spasojević-Kosić, Ljubica, Bacić, Dragan, Cincović, Marko, Toholj, Bojan, Stevančević, Ognjen, Ristić, Zoran, "Occurrence of Bartonella henselae, FeLV and FIV infection in 60 stray cats from Serbia" in Acta Veterinaria-Beograd, 64, no. 3 (2014):378-385,
https://doi.org/10.2478/acve-2014-0036 . .