TY  - JOUR
AU  - Janjić, Filip
AU  - Beletić, Anđelo
AU  - Radaković, Milena
AU  - Spariosu, Kristina
AU  - Diklić, Miloš
AU  - Francuski Andrić, Jelena
AU  - Radonjić, Vladimir
AU  - Ajtić, Jelena
AU  - Kovačević-Filipović, Milica
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2338
AB  - The highest number of acute Babesia canis cases in dogs is recorded over the February-May (Feb-May) period, which also represents the optimal climate conditions for tick activity in Belgrade, Serbia. A possibility that the acute phase response is more intense in dogs developing the disease in the Feb-May period compared with the response in other time periods of the year was tested. A total of 63 client-owned dogs with acute B. canis infection were enrolled and the routine hematology and biochemistry parameters-serum amyloid A (SAA), IgG against B. canis, level of parasitemia, ceruloplasmin (CER), paraoxonase-1 (PON-1), and fibrinogen-were measured. Acute phase indexes (API) were calculated as (SAA×CER)/(Iron×PON-1) and (SAA×CER)/(Albumin×Iron). Statistics included Kruskal-Wallis test and logistic regression analysis. The results showed that in the Feb-May period, the following parameters were lower: creatinine, albumin, iron, and level of parasitemia. Furthermore, increased API values were more probable in the Feb-May than in the other periods. Together, higher acute phase response intensity and presumptive hemodilution in the Feb-May period indicate a more acute course of B. canis infection than in other time periods of the year.
PB  - Springer
T2  - International Journal of Biometeorology
T1  - Seasonal differences in the intensity of acute phase response in dogs infected with Babesia canis
DO  - 10.1007/s00484-021-02228-4
ER  - 

@article{
author = "Janjić, Filip and Beletić, Anđelo and Radaković, Milena and Spariosu, Kristina and Diklić, Miloš and Francuski Andrić, Jelena and Radonjić, Vladimir and Ajtić, Jelena and Kovačević-Filipović, Milica",
year = "2022",
abstract = "The highest number of acute Babesia canis cases in dogs is recorded over the February-May (Feb-May) period, which also represents the optimal climate conditions for tick activity in Belgrade, Serbia. A possibility that the acute phase response is more intense in dogs developing the disease in the Feb-May period compared with the response in other time periods of the year was tested. A total of 63 client-owned dogs with acute B. canis infection were enrolled and the routine hematology and biochemistry parameters-serum amyloid A (SAA), IgG against B. canis, level of parasitemia, ceruloplasmin (CER), paraoxonase-1 (PON-1), and fibrinogen-were measured. Acute phase indexes (API) were calculated as (SAA×CER)/(Iron×PON-1) and (SAA×CER)/(Albumin×Iron). Statistics included Kruskal-Wallis test and logistic regression analysis. The results showed that in the Feb-May period, the following parameters were lower: creatinine, albumin, iron, and level of parasitemia. Furthermore, increased API values were more probable in the Feb-May than in the other periods. Together, higher acute phase response intensity and presumptive hemodilution in the Feb-May period indicate a more acute course of B. canis infection than in other time periods of the year.",
publisher = "Springer",
journal = "International Journal of Biometeorology",
title = "Seasonal differences in the intensity of acute phase response in dogs infected with Babesia canis",
doi = "10.1007/s00484-021-02228-4"
}

Janjić, F., Beletić, A., Radaković, M., Spariosu, K., Diklić, M., Francuski Andrić, J., Radonjić, V., Ajtić, J.,& Kovačević-Filipović, M.. (2022). Seasonal differences in the intensity of acute phase response in dogs infected with Babesia canis. in International Journal of Biometeorology
Springer..
https://doi.org/10.1007/s00484-021-02228-4

Janjić F, Beletić A, Radaković M, Spariosu K, Diklić M, Francuski Andrić J, Radonjić V, Ajtić J, Kovačević-Filipović M. Seasonal differences in the intensity of acute phase response in dogs infected with Babesia canis. in International Journal of Biometeorology. 2022;.
doi:10.1007/s00484-021-02228-4 .

Janjić, Filip, Beletić, Anđelo, Radaković, Milena, Spariosu, Kristina, Diklić, Miloš, Francuski Andrić, Jelena, Radonjić, Vladimir, Ajtić, Jelena, Kovačević-Filipović, Milica, "Seasonal differences in the intensity of acute phase response in dogs infected with Babesia canis" in International Journal of Biometeorology (2022),
https://doi.org/10.1007/s00484-021-02228-4 . .

TY  - CONF
AU  - Janjić, Filip
AU  - Beletić, Anđelo
AU  - Radaković, Milena
AU  - Spariosu, Kristina
AU  - Francuski Andrić, Jelena
AU  - Ajtić, Jelena
AU  - Kovačević-Filipović, Milica
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2488
AB  - Background: Canine babesiosis is a disease with a seasonal character. We have
previously observed that in Belgrade, the capital of the Republic of Serbia, 60% of
cases with the acute-phase response (APR) give a notable maximum between mid-
February and May, the so-called “high season”. The other two peak seasons with
less pronounced maxima are in winter, from January to mid-February, and in
autumn, from October to December. Objectives: Based on clinical observations, we
postulate that dogs infected with B. canis in the “high season” develop a more
intense APR than dogs in the other two seasons. Material and Methods: To test this
hypothesis, we compare the relative parasite load and the level of acute-phase
proteins and reactants, their indexes (calculated as a combination of
concentrations of fast and slow positive, and fast and slow negative acute phase
reactants), as well as the concentration of coagulation factors, complete blood
count, and biochemistry among dogs that developed the disease in the autumn,
winter, and “high season”. Results: The relative parasite load is the lowest, and APR
Indexes are the highest in dogs infected in “high season”. These findings are
accompanied by relatively low creatinine and urea, implying that dogs in “high
season” had hemodilution, thus further suggesting short incubation. Unlike other
acute-phase proteins, fibrinogen showed the lowest concentration in “high
season”. Conclusion: Future research will help unveil the connection between
more intense APR and relatively low parasite load and fibrinogen concentration.
C3  - 24th Annual Congress of the European Society of Veterinary Clinical Pathology, Belgrade, Serbia, October 5-8
T1  - Canine babesiosis in different seasons - a fertile ground for multiple questions
SP  - 78
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2488
ER  - 

@conference{
author = "Janjić, Filip and Beletić, Anđelo and Radaković, Milena and Spariosu, Kristina and Francuski Andrić, Jelena and Ajtić, Jelena and Kovačević-Filipović, Milica",
year = "2022",
abstract = "Background: Canine babesiosis is a disease with a seasonal character. We have
previously observed that in Belgrade, the capital of the Republic of Serbia, 60% of
cases with the acute-phase response (APR) give a notable maximum between mid-
February and May, the so-called “high season”. The other two peak seasons with
less pronounced maxima are in winter, from January to mid-February, and in
autumn, from October to December. Objectives: Based on clinical observations, we
postulate that dogs infected with B. canis in the “high season” develop a more
intense APR than dogs in the other two seasons. Material and Methods: To test this
hypothesis, we compare the relative parasite load and the level of acute-phase
proteins and reactants, their indexes (calculated as a combination of
concentrations of fast and slow positive, and fast and slow negative acute phase
reactants), as well as the concentration of coagulation factors, complete blood
count, and biochemistry among dogs that developed the disease in the autumn,
winter, and “high season”. Results: The relative parasite load is the lowest, and APR
Indexes are the highest in dogs infected in “high season”. These findings are
accompanied by relatively low creatinine and urea, implying that dogs in “high
season” had hemodilution, thus further suggesting short incubation. Unlike other
acute-phase proteins, fibrinogen showed the lowest concentration in “high
season”. Conclusion: Future research will help unveil the connection between
more intense APR and relatively low parasite load and fibrinogen concentration.",
journal = "24th Annual Congress of the European Society of Veterinary Clinical Pathology, Belgrade, Serbia, October 5-8",
title = "Canine babesiosis in different seasons - a fertile ground for multiple questions",
pages = "78-79",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2488"
}

Janjić, F., Beletić, A., Radaković, M., Spariosu, K., Francuski Andrić, J., Ajtić, J.,& Kovačević-Filipović, M.. (2022). Canine babesiosis in different seasons - a fertile ground for multiple questions. in 24th Annual Congress of the European Society of Veterinary Clinical Pathology, Belgrade, Serbia, October 5-8, 78-79.
https://hdl.handle.net/21.15107/rcub_veterinar_2488

Janjić F, Beletić A, Radaković M, Spariosu K, Francuski Andrić J, Ajtić J, Kovačević-Filipović M. Canine babesiosis in different seasons - a fertile ground for multiple questions. in 24th Annual Congress of the European Society of Veterinary Clinical Pathology, Belgrade, Serbia, October 5-8. 2022;:78-79.
https://hdl.handle.net/21.15107/rcub_veterinar_2488 .

Janjić, Filip, Beletić, Anđelo, Radaković, Milena, Spariosu, Kristina, Francuski Andrić, Jelena, Ajtić, Jelena, Kovačević-Filipović, Milica, "Canine babesiosis in different seasons - a fertile ground for multiple questions" in 24th Annual Congress of the European Society of Veterinary Clinical Pathology, Belgrade, Serbia, October 5-8 (2022):78-79,
https://hdl.handle.net/21.15107/rcub_veterinar_2488 .

TY  - CONF
AU  - Janjić, Filip
AU  - Spariosu, Kristina
AU  - Kitanović, Sara
AU  - Radaković, Milena
AU  - Francuski Andrić, Jelena
AU  - Beletić, Anđelo
AU  - Kovačević Filipović, Milica
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2586
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 33. Savetovanje Veterinara Srbije, Zlatibor, 08 - 11. Septembar 2022
T1  - Retrospektivna analiza prevalence anemija i faktori rizika kod pasa i mačaka u toku 2021–2022. godine na klinici za male životinje Fakulteta Veterinarske Medicine Univerziteta u Beogradu
SP  - 312
EP  - 313
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2586
ER  - 

@conference{
author = "Janjić, Filip and Spariosu, Kristina and Kitanović, Sara and Radaković, Milena and Francuski Andrić, Jelena and Beletić, Anđelo and Kovačević Filipović, Milica",
year = "2022",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "33. Savetovanje Veterinara Srbije, Zlatibor, 08 - 11. Septembar 2022",
title = "Retrospektivna analiza prevalence anemija i faktori rizika kod pasa i mačaka u toku 2021–2022. godine na klinici za male životinje Fakulteta Veterinarske Medicine Univerziteta u Beogradu",
pages = "312-313",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2586"
}

Janjić, F., Spariosu, K., Kitanović, S., Radaković, M., Francuski Andrić, J., Beletić, A.,& Kovačević Filipović, M.. (2022). Retrospektivna analiza prevalence anemija i faktori rizika kod pasa i mačaka u toku 2021–2022. godine na klinici za male životinje Fakulteta Veterinarske Medicine Univerziteta u Beogradu. in 33. Savetovanje Veterinara Srbije, Zlatibor, 08 - 11. Septembar 2022
Beograd : Srpsko veterinarsko društvo., 312-313.
https://hdl.handle.net/21.15107/rcub_veterinar_2586

Janjić F, Spariosu K, Kitanović S, Radaković M, Francuski Andrić J, Beletić A, Kovačević Filipović M. Retrospektivna analiza prevalence anemija i faktori rizika kod pasa i mačaka u toku 2021–2022. godine na klinici za male životinje Fakulteta Veterinarske Medicine Univerziteta u Beogradu. in 33. Savetovanje Veterinara Srbije, Zlatibor, 08 - 11. Septembar 2022. 2022;:312-313.
https://hdl.handle.net/21.15107/rcub_veterinar_2586 .

Janjić, Filip, Spariosu, Kristina, Kitanović, Sara, Radaković, Milena, Francuski Andrić, Jelena, Beletić, Anđelo, Kovačević Filipović, Milica, "Retrospektivna analiza prevalence anemija i faktori rizika kod pasa i mačaka u toku 2021–2022. godine na klinici za male životinje Fakulteta Veterinarske Medicine Univerziteta u Beogradu" in 33. Savetovanje Veterinara Srbije, Zlatibor, 08 - 11. Septembar 2022 (2022):312-313,
https://hdl.handle.net/21.15107/rcub_veterinar_2586 .

TY  - JOUR
AU  - Spariosu, Kristina
AU  - Janjić, Filip
AU  - Francuski Andrić, Jelena
AU  - Radaković, Milena
AU  - Beletić, Anđelo
AU  - Kovačević-Filipović, Milica
AU  - Milanović, Svetlana
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2132
AB  - Hemolysis and systemic acute inflammation characterize canine babesiosis caused by the intraerythrocytic protozoan parasite Babesia canis. Our hypothesis was that blood redox homeostasis of patients that suffered acute B. canis infection might be disturbed even after treatment with imidocarb-dipropionate and successful clinical recovery. Eight owner dogs with acute B. canis infection were used for this study. We analyzed the complete blood count, acute phase proteins (ceruloplasmin, haptoglobin, paraoxonase-1) in the serum, antioxidant enzymes (catalase and glutathione peroxidase) in the erythrocytes, and oxidative stress markers (malondialdehyde in erythrocytes and thiol groups in serum) at presentation and 15 days after treatment. Results were evaluated by corresponding statistical tests. At presentation, anemia, low/normal leukocyte count and severe thrombocytopenia occurred together with increased ceruloplasmin, haptoglobin levels within the reference interval, decreased paraoxonase-1 and compromised antioxidant defense in the red blood cells. After treatment and successful clinical recovery, hematological values generally fitted within the reference intervals, acute phase proteins were within the physiological levels in the majority of cases and the activities of the antioxidant enzymes were increased. However, elevated malondialdehyde levels indicated increased oxidative damage of erythrocytes that remained as a deleterious sequel despite a successful clinical recovery of the dogs.
AB  - Babezioza pasa, koju izaziva protozoa Babesia canis, praćena je hemolizom i sistemskom akutnom infl amacijom. Naša hipoteza je da kod pasa sa akutnom B. canis infekcijom, čak i nakon terapije imidokarb-dipropionatom i uspešnog kliničkog oporavka, postoji poremećaj redoks homeostaze. Studija uključuje osam vlasničkih pasa akutno infi ciranih babezijom. Analizirani su kompletna krvna slika, proteini akutne faze (ceruloplazmin, haptoglobin i paraoksonaza-1) u serumu, antioksidativni enzimi (katalaza i glutation peroksidaza) u eritrocitima i markeri oksidativnog stresa (malondialdehid u eritrocitima, tiolne grupe u serumu) na prezentaciji i 15 dana nakon terapije. Za procenu značajnosti rezultata primenjeni su odgovarajući statistički testovi. Anemija, nizak/normalan broj leukocita i teška trombocitopenija praćeni povišenom koncentracijom ceruloplazmina, haptoglobinom u fi ziološkim intervalima i sniženim nivoom paraoksonaze-1, kao i kompromitovana antioksidativna zaštita, uočeni su na prezentaciji. Nakon terapije i uspešnog kliničkog oporavka, hematološke vrednosti su bile u fi ziološkom opsegu, kao i proteini akutne faze kod većine pacijenata, a aktivnost antioksidativnih enzima je pojačana. Međutim, povišena koncentracija malondialdehida ukazuje na povećano oštećenje eritrocita koje je zaostalo uprkos uspešnom kliničkom oporavku pasa.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Relationship between changes in hematological parameters, levels of acute phase proteins and redox homeostasis during acute Babesia canis infection in dogs
T1  - Povezanost promena hematoloških parametara, nivoa proteina akutne faze i redoks homeostaze tokom akutne infekcije pasa sa babesia canis
VL  - 71
IS  - 2
SP  - 158
EP  - 169
DO  - 10.2478/acve-2021-0014
ER  - 

@article{
author = "Spariosu, Kristina and Janjić, Filip and Francuski Andrić, Jelena and Radaković, Milena and Beletić, Anđelo and Kovačević-Filipović, Milica and Milanović, Svetlana",
year = "2021",
abstract = "Hemolysis and systemic acute inflammation characterize canine babesiosis caused by the intraerythrocytic protozoan parasite Babesia canis. Our hypothesis was that blood redox homeostasis of patients that suffered acute B. canis infection might be disturbed even after treatment with imidocarb-dipropionate and successful clinical recovery. Eight owner dogs with acute B. canis infection were used for this study. We analyzed the complete blood count, acute phase proteins (ceruloplasmin, haptoglobin, paraoxonase-1) in the serum, antioxidant enzymes (catalase and glutathione peroxidase) in the erythrocytes, and oxidative stress markers (malondialdehyde in erythrocytes and thiol groups in serum) at presentation and 15 days after treatment. Results were evaluated by corresponding statistical tests. At presentation, anemia, low/normal leukocyte count and severe thrombocytopenia occurred together with increased ceruloplasmin, haptoglobin levels within the reference interval, decreased paraoxonase-1 and compromised antioxidant defense in the red blood cells. After treatment and successful clinical recovery, hematological values generally fitted within the reference intervals, acute phase proteins were within the physiological levels in the majority of cases and the activities of the antioxidant enzymes were increased. However, elevated malondialdehyde levels indicated increased oxidative damage of erythrocytes that remained as a deleterious sequel despite a successful clinical recovery of the dogs., Babezioza pasa, koju izaziva protozoa Babesia canis, praćena je hemolizom i sistemskom akutnom infl amacijom. Naša hipoteza je da kod pasa sa akutnom B. canis infekcijom, čak i nakon terapije imidokarb-dipropionatom i uspešnog kliničkog oporavka, postoji poremećaj redoks homeostaze. Studija uključuje osam vlasničkih pasa akutno infi ciranih babezijom. Analizirani su kompletna krvna slika, proteini akutne faze (ceruloplazmin, haptoglobin i paraoksonaza-1) u serumu, antioksidativni enzimi (katalaza i glutation peroksidaza) u eritrocitima i markeri oksidativnog stresa (malondialdehid u eritrocitima, tiolne grupe u serumu) na prezentaciji i 15 dana nakon terapije. Za procenu značajnosti rezultata primenjeni su odgovarajući statistički testovi. Anemija, nizak/normalan broj leukocita i teška trombocitopenija praćeni povišenom koncentracijom ceruloplazmina, haptoglobinom u fi ziološkim intervalima i sniženim nivoom paraoksonaze-1, kao i kompromitovana antioksidativna zaštita, uočeni su na prezentaciji. Nakon terapije i uspešnog kliničkog oporavka, hematološke vrednosti su bile u fi ziološkom opsegu, kao i proteini akutne faze kod većine pacijenata, a aktivnost antioksidativnih enzima je pojačana. Međutim, povišena koncentracija malondialdehida ukazuje na povećano oštećenje eritrocita koje je zaostalo uprkos uspešnom kliničkom oporavku pasa.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Relationship between changes in hematological parameters, levels of acute phase proteins and redox homeostasis during acute Babesia canis infection in dogs, Povezanost promena hematoloških parametara, nivoa proteina akutne faze i redoks homeostaze tokom akutne infekcije pasa sa babesia canis",
volume = "71",
number = "2",
pages = "158-169",
doi = "10.2478/acve-2021-0014"
}

Spariosu, K., Janjić, F., Francuski Andrić, J., Radaković, M., Beletić, A., Kovačević-Filipović, M.,& Milanović, S.. (2021). Relationship between changes in hematological parameters, levels of acute phase proteins and redox homeostasis during acute Babesia canis infection in dogs. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(2), 158-169.
https://doi.org/10.2478/acve-2021-0014

Spariosu K, Janjić F, Francuski Andrić J, Radaković M, Beletić A, Kovačević-Filipović M, Milanović S. Relationship between changes in hematological parameters, levels of acute phase proteins and redox homeostasis during acute Babesia canis infection in dogs. in Acta Veterinaria-Beograd. 2021;71(2):158-169.
doi:10.2478/acve-2021-0014 .

Spariosu, Kristina, Janjić, Filip, Francuski Andrić, Jelena, Radaković, Milena, Beletić, Anđelo, Kovačević-Filipović, Milica, Milanović, Svetlana, "Relationship between changes in hematological parameters, levels of acute phase proteins and redox homeostasis during acute Babesia canis infection in dogs" in Acta Veterinaria-Beograd, 71, no. 2 (2021):158-169,
https://doi.org/10.2478/acve-2021-0014 . .

TY  - JOUR
AU  - Beletić, Anđelo
AU  - Janjić, Filip
AU  - Radaković, Milena
AU  - Spariosu, Kristina
AU  - Francuski Andrić, Jelena
AU  - Chandrashekar, Ramaswamy
AU  - Tyrrell, Phyllis
AU  - Radonjić, Vladimir
AU  - Balint, Bela
AU  - Ajtić, Jelena
AU  - Kovačević-Filipović, Milica
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2015
AB  - The common signs of canine babesiosis caused by an infection with Babesia canis are fever, anorexia, lethargy, pulse alterations, anemia, and occasionally mild icterus. Dogs with these clinical signs can be divided into two groups: those with acute-phase reaction and those with systemic inflammatory response syndrome (SIRS). Factors associated with the occurrence of SIRS in canine babesiosis have not been thoroughly researched. This article outlines a cross-sectional study of 54 client-owned dogs with an acute B. canis infection, and evaluates the differences in age, gender, laboratory findings, parasite load, and seroreactivity against B. canis between the SIRS and the SIRS-free dogs. We have analyzed a complete blood count, serum biochemistry, serum amyloid A, ceruloplasmin, paraoxonase-1, serology, and PCR testing using standard methodologies. The frequency of SIRS among the investigated dogs reached 0.59. Male dogs and those seronegative against B. canis, were more frequent in the SIRS group, whilst age and parasite load could not be associated with the presence of SIRS. Dogs with SIRS had a lower count of total leukocytes, neutrophils, lymphocytes, and monocytes, and a lower concentration of iron and bilirubin compared with SIRS-free dogs. No significant differences in the concentration of acute-phase proteins have been noticed to exist between the groups of dogs. Further, the seronegative dogs had a lower count of lymphocytes and monocytes and a higher parasite load than the seroreactive dogs. Multivariate logistic regression analysis has identified leukopenia (<6 × 109/L) and monocytopenia (<0.2 × 109/L) as independent associates of SIRS in the investigated dogs, thus implying that these routine tests could be used as reliable markers for SIRS.
PB  - Elsevier
T2  - Veterinary Parasitology
T1  - Systemic inflammatory response syndrome in dogs naturally infected with Babesia canis: Association with the parasite load and host factors
VL  - 291
DO  - 10.1016/j.vetpar.2021.109366
ER  - 

@article{
author = "Beletić, Anđelo and Janjić, Filip and Radaković, Milena and Spariosu, Kristina and Francuski Andrić, Jelena and Chandrashekar, Ramaswamy and Tyrrell, Phyllis and Radonjić, Vladimir and Balint, Bela and Ajtić, Jelena and Kovačević-Filipović, Milica",
year = "2021",
abstract = "The common signs of canine babesiosis caused by an infection with Babesia canis are fever, anorexia, lethargy, pulse alterations, anemia, and occasionally mild icterus. Dogs with these clinical signs can be divided into two groups: those with acute-phase reaction and those with systemic inflammatory response syndrome (SIRS). Factors associated with the occurrence of SIRS in canine babesiosis have not been thoroughly researched. This article outlines a cross-sectional study of 54 client-owned dogs with an acute B. canis infection, and evaluates the differences in age, gender, laboratory findings, parasite load, and seroreactivity against B. canis between the SIRS and the SIRS-free dogs. We have analyzed a complete blood count, serum biochemistry, serum amyloid A, ceruloplasmin, paraoxonase-1, serology, and PCR testing using standard methodologies. The frequency of SIRS among the investigated dogs reached 0.59. Male dogs and those seronegative against B. canis, were more frequent in the SIRS group, whilst age and parasite load could not be associated with the presence of SIRS. Dogs with SIRS had a lower count of total leukocytes, neutrophils, lymphocytes, and monocytes, and a lower concentration of iron and bilirubin compared with SIRS-free dogs. No significant differences in the concentration of acute-phase proteins have been noticed to exist between the groups of dogs. Further, the seronegative dogs had a lower count of lymphocytes and monocytes and a higher parasite load than the seroreactive dogs. Multivariate logistic regression analysis has identified leukopenia (<6 × 109/L) and monocytopenia (<0.2 × 109/L) as independent associates of SIRS in the investigated dogs, thus implying that these routine tests could be used as reliable markers for SIRS.",
publisher = "Elsevier",
journal = "Veterinary Parasitology",
title = "Systemic inflammatory response syndrome in dogs naturally infected with Babesia canis: Association with the parasite load and host factors",
volume = "291",
doi = "10.1016/j.vetpar.2021.109366"
}

Beletić, A., Janjić, F., Radaković, M., Spariosu, K., Francuski Andrić, J., Chandrashekar, R., Tyrrell, P., Radonjić, V., Balint, B., Ajtić, J.,& Kovačević-Filipović, M.. (2021). Systemic inflammatory response syndrome in dogs naturally infected with Babesia canis: Association with the parasite load and host factors. in Veterinary Parasitology
Elsevier., 291.
https://doi.org/10.1016/j.vetpar.2021.109366

Beletić A, Janjić F, Radaković M, Spariosu K, Francuski Andrić J, Chandrashekar R, Tyrrell P, Radonjić V, Balint B, Ajtić J, Kovačević-Filipović M. Systemic inflammatory response syndrome in dogs naturally infected with Babesia canis: Association with the parasite load and host factors. in Veterinary Parasitology. 2021;291.
doi:10.1016/j.vetpar.2021.109366 .

Beletić, Anđelo, Janjić, Filip, Radaković, Milena, Spariosu, Kristina, Francuski Andrić, Jelena, Chandrashekar, Ramaswamy, Tyrrell, Phyllis, Radonjić, Vladimir, Balint, Bela, Ajtić, Jelena, Kovačević-Filipović, Milica, "Systemic inflammatory response syndrome in dogs naturally infected with Babesia canis: Association with the parasite load and host factors" in Veterinary Parasitology, 291 (2021),
https://doi.org/10.1016/j.vetpar.2021.109366 . .

TY  - JOUR
AU  - Spariosu, Kristina
AU  - Janjić, Filip
AU  - Radaković, Milena
AU  - Francuski Andrić, Jelena
AU  - Beletić, Anđelo
AU  - Kovačević-Filipović, Milica
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2240
AB  - Inflammation is a hallmark of the acute Babesia canis infection. Promatrix metalloproteinase (proMMP)-2 and -9 are involved in inflammation, but their levels have not been analyzed in canine babesiosis. We hypothesized that in dogs infected with B. canis, serum proMMP-2 and -9 levels change between presentation and recovery. Degree of the change differs if dogs develop systemic inflammatory response syndrome (SIRS). This study included 24 dogs with an acute B. canis infection, at presentation and after two weeks. We used routine hematology and biochemistry methods, spectrophotometry for the acute-phase proteins, microscopy for parasitemia and zymography for (pro)MMPs. In vitro endothelial cells and leukocyte short-term cultures, and platelet lysates were used to detect specific MMP activity. Statistical analyses included Wilcoxon test for paired samples, Mann-Whitney U test and Spearman’s rank correlation. Our results showed that endothelial cells, leukocytes and platelets are the source of proMMP-2 and proMMP-9. Furthermore, both proMMPs were lower at presentation than after recovery (p < 0.001). At presentation, proMMP-9 levels correlated with parasitemia (rho = -0.616, p = 0.009), total leukocyte (rho = 0.704, p < 0.001) and neutrophil counts (rho = 0.741, p < 0.001). Extent of alterations in proMMP-2 levels between presentation and recovery was lower (p = 0.038) in dogs with SIRS than in non-SIRS dogs, while levels of proMMP-9 were comparable between these groups. Our conclusion is that during the acute B. canis infection, low serum levels of proMMP-2 and proMMP-9 at presentation reflect thrombocytopenia and leukopenia. Decreased proMMP-2 level could be associated with SIRS.
PB  - Elsevier
T2  - Veterinary Parasitology
T1  - Low serum levels of promatrix metalloproteinase-2 and -9 occur during acute Babesia canis infection in dogs
VL  - 300
SP  - 109612
DO  - 10.1016/j.vetpar.2021.109612
ER  - 

@article{
author = "Spariosu, Kristina and Janjić, Filip and Radaković, Milena and Francuski Andrić, Jelena and Beletić, Anđelo and Kovačević-Filipović, Milica",
year = "2021",
abstract = "Inflammation is a hallmark of the acute Babesia canis infection. Promatrix metalloproteinase (proMMP)-2 and -9 are involved in inflammation, but their levels have not been analyzed in canine babesiosis. We hypothesized that in dogs infected with B. canis, serum proMMP-2 and -9 levels change between presentation and recovery. Degree of the change differs if dogs develop systemic inflammatory response syndrome (SIRS). This study included 24 dogs with an acute B. canis infection, at presentation and after two weeks. We used routine hematology and biochemistry methods, spectrophotometry for the acute-phase proteins, microscopy for parasitemia and zymography for (pro)MMPs. In vitro endothelial cells and leukocyte short-term cultures, and platelet lysates were used to detect specific MMP activity. Statistical analyses included Wilcoxon test for paired samples, Mann-Whitney U test and Spearman’s rank correlation. Our results showed that endothelial cells, leukocytes and platelets are the source of proMMP-2 and proMMP-9. Furthermore, both proMMPs were lower at presentation than after recovery (p < 0.001). At presentation, proMMP-9 levels correlated with parasitemia (rho = -0.616, p = 0.009), total leukocyte (rho = 0.704, p < 0.001) and neutrophil counts (rho = 0.741, p < 0.001). Extent of alterations in proMMP-2 levels between presentation and recovery was lower (p = 0.038) in dogs with SIRS than in non-SIRS dogs, while levels of proMMP-9 were comparable between these groups. Our conclusion is that during the acute B. canis infection, low serum levels of proMMP-2 and proMMP-9 at presentation reflect thrombocytopenia and leukopenia. Decreased proMMP-2 level could be associated with SIRS.",
publisher = "Elsevier",
journal = "Veterinary Parasitology",
title = "Low serum levels of promatrix metalloproteinase-2 and -9 occur during acute Babesia canis infection in dogs",
volume = "300",
pages = "109612",
doi = "10.1016/j.vetpar.2021.109612"
}

Spariosu, K., Janjić, F., Radaković, M., Francuski Andrić, J., Beletić, A.,& Kovačević-Filipović, M.. (2021). Low serum levels of promatrix metalloproteinase-2 and -9 occur during acute Babesia canis infection in dogs. in Veterinary Parasitology
Elsevier., 300, 109612.
https://doi.org/10.1016/j.vetpar.2021.109612

Spariosu K, Janjić F, Radaković M, Francuski Andrić J, Beletić A, Kovačević-Filipović M. Low serum levels of promatrix metalloproteinase-2 and -9 occur during acute Babesia canis infection in dogs. in Veterinary Parasitology. 2021;300:109612.
doi:10.1016/j.vetpar.2021.109612 .

Spariosu, Kristina, Janjić, Filip, Radaković, Milena, Francuski Andrić, Jelena, Beletić, Anđelo, Kovačević-Filipović, Milica, "Low serum levels of promatrix metalloproteinase-2 and -9 occur during acute Babesia canis infection in dogs" in Veterinary Parasitology, 300 (2021):109612,
https://doi.org/10.1016/j.vetpar.2021.109612 . .

TY  - JOUR
AU  - Milanović, Zorana
AU  - Beletić, Anđelo
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Andrić, Nenad
AU  - Božović, Anja Ilić
AU  - Spariosu, Kristina
AU  - Radaković, Milena
AU  - Ajtić, Jelena
AU  - Kovačević-Filipović, Milica
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1875
AB  - Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.
PB  - Elsevier Science Bv, Amsterdam
T2  - Veterinary Parasitology
T1  - Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis
VL  - 282
SP  - 109140
DO  - 10.1016/j.vetpar.2020.109140
ER  - 

@article{
author = "Milanović, Zorana and Beletić, Anđelo and Vekić, Jelena and Zeljković, Aleksandra and Andrić, Nenad and Božović, Anja Ilić and Spariosu, Kristina and Radaković, Milena and Ajtić, Jelena and Kovačević-Filipović, Milica",
year = "2020",
abstract = "Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Veterinary Parasitology",
title = "Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis",
volume = "282",
pages = "109140",
doi = "10.1016/j.vetpar.2020.109140"
}

Milanović, Z., Beletić, A., Vekić, J., Zeljković, A., Andrić, N., Božović, A. I., Spariosu, K., Radaković, M., Ajtić, J.,& Kovačević-Filipović, M.. (2020). Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis. in Veterinary Parasitology
Elsevier Science Bv, Amsterdam., 282, 109140.
https://doi.org/10.1016/j.vetpar.2020.109140

Milanović Z, Beletić A, Vekić J, Zeljković A, Andrić N, Božović AI, Spariosu K, Radaković M, Ajtić J, Kovačević-Filipović M. Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis. in Veterinary Parasitology. 2020;282:109140.
doi:10.1016/j.vetpar.2020.109140 .

Milanović, Zorana, Beletić, Anđelo, Vekić, Jelena, Zeljković, Aleksandra, Andrić, Nenad, Božović, Anja Ilić, Spariosu, Kristina, Radaković, Milena, Ajtić, Jelena, Kovačević-Filipović, Milica, "Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis" in Veterinary Parasitology, 282 (2020):109140,
https://doi.org/10.1016/j.vetpar.2020.109140 . .

TY  - JOUR
AU  - Janjić, Filip
AU  - Milanović, Zorana
AU  - Ilić Božović, Anja
AU  - Andrić, Nenad
AU  - Spariosu, Kristina
AU  - Ajtić, Jelena
AU  - Beletić, Anđelo
AU  - Kovačević-Filipović, Milica
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2214
AB  - Haematologic abnormalities possibly associated with exposure to vector-borne pathogens are rarely reported in clinically healthy outdoor dogs. Therefore, we analysed changes in the complete blood count (CBC) of clinically healthy outdoor dogs seroreactive to Anaplasma spp. and Babesia spp., with or without microfilariosis. Stray, shelter and hunting dogs, 81 in total, that were polymerase chain reaction negative for Anaplasma spp. and Babesia spp. were divided into groups according to their seroreactive status and results of a modified Knott’s test: seronegative to both Anaplasma spp. and Babesia spp. SN (N=26); seroreactive to A. phagocythophilum SR-A (N=12); seroreactive to B. canis, B. gibsoni and/or B. vogeli SR-B (N=25); and seroreactive to both of the pathogens SR-AB (N=8). These four groups were negative to microfilariosis, unlike the fifth group, seroreactive to either or to both of the pathogens and with microfilariosis SR-M (N=10). The frequencies of CBC alterations among all analysed dogs were: 0.35 – leucocytosis, 0.44 – granulocytosis, 0.28 – anaemia, 0.74 – microcytosis, 0.37 – increased mean cell haemoglobin concentration (MCHC) and 0.33 – thrombocytopenia. The frequency of alterations did not differ across the groups. An exception was the SR-M group wherein increased MCHC peaked with a frequency of 0.80, while in the 
other four groups, the frequency ranged between 0.10 and 0.50. Clinically healthy outdoor dogs have multiple CBC abnormalities, consistent with stress and low-level chronic inflammation, but not associated with a previous exposure to Anaplasma spp. or Babesia spp. The presence of microfilaria increases haemolysis in vitro.
AB  - Klinički zdravi psi koji borave na otvorenom i koji su izloženi patogenima koje prenose vektori mogu imati hematološke premećaje vezane za dejstvo tih patogena. Ipak, hematološke pretrage se retko izvode na toj populaciji pasa. Stoga je cilj našeg rada bio da utvrdimo promene u krvnoj slici zdravih pasa lutalica, pasa smeštenih u azilima i lovačkih pasa, seroreaktivnih na uzročnike bolesti iz rodova Anaplasma i Babesia, sa ili bez mikrofilarioze. U ovom radu je ispitana krvna slika 81 psa. U studiju su uključeni psi kod kojih lančanom reakcijom polimerizacije nije pokazano prisustvo DNK Anaplasma spp. i Babesia spp. Psi su prema serološkom statusu raspoređeni u sledeće grupe: seronegativni na Anaplasma spp. i Babesia spp. SN (N=26), seroreaktivni na A. phagocytophilum SR-A (N=12), seroreaktivni na B. canis, B. gibsoni i/ili B. vogeli SR-B (N=25) i seroreaktivni na oba navedena patogena SR-AB (N=8). Ove četiri grupe su bile negativne na mikrofilariozu, dok je peta grupa, seroreaktivna na neki ili oba patogena imala i mikrofilarije SR-M (N=10). Učestalost promena kompletne krvne slike među svim ispitivanim psima je bila sledeća: 0,35 za leukocitozu, 0,44 za granulocitozu, 0,28 za anemiju, 0,74 za mikrocitozu, 0,37 za povećanu prosečnu koncentraciju hemoglobina u eritrocitima i 0,33 za trombocitopeniju. Grupe se nisu razlikovale u pogledu učestalosti navedenih promena. Izuzetak je grupa SR-M u kojoj je učestalost povećanja vrednosti za prosečnu koncentraciju hemoglobina u eritrocitima iznosila 0,80. U ostale četiri grupe ta učestalost je iznosila između 0,10 i 0,50. Zaključak je da ispitivani psi imaju višestruke poremećaje krvne slike koji najverovatnije odražavaju stres i nizak stepen hronične inflamacije, ali ne i vezu sa prethodnim kontaktom sa Anaplasma spp. i Babesia spp. Prisustvo mikrofilarija izaziva hemolizu in vitro.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Hаematologic indices in clinically healthy outdoor dogs exposed to vector-borne pathogens
T1  - Hematološki parametri kod klinički zdravih pasa koji borave na otvorenom i izloženi su patogenima koje prenose vektori
VL  - 74
IS  - 2
SP  - 178
EP  - 186
DO  - 10.2298/VETGL191216005J
ER  - 

@article{
author = "Janjić, Filip and Milanović, Zorana and Ilić Božović, Anja and Andrić, Nenad and Spariosu, Kristina and Ajtić, Jelena and Beletić, Anđelo and Kovačević-Filipović, Milica",
year = "2020",
abstract = "Haematologic abnormalities possibly associated with exposure to vector-borne pathogens are rarely reported in clinically healthy outdoor dogs. Therefore, we analysed changes in the complete blood count (CBC) of clinically healthy outdoor dogs seroreactive to Anaplasma spp. and Babesia spp., with or without microfilariosis. Stray, shelter and hunting dogs, 81 in total, that were polymerase chain reaction negative for Anaplasma spp. and Babesia spp. were divided into groups according to their seroreactive status and results of a modified Knott’s test: seronegative to both Anaplasma spp. and Babesia spp. SN (N=26); seroreactive to A. phagocythophilum SR-A (N=12); seroreactive to B. canis, B. gibsoni and/or B. vogeli SR-B (N=25); and seroreactive to both of the pathogens SR-AB (N=8). These four groups were negative to microfilariosis, unlike the fifth group, seroreactive to either or to both of the pathogens and with microfilariosis SR-M (N=10). The frequencies of CBC alterations among all analysed dogs were: 0.35 – leucocytosis, 0.44 – granulocytosis, 0.28 – anaemia, 0.74 – microcytosis, 0.37 – increased mean cell haemoglobin concentration (MCHC) and 0.33 – thrombocytopenia. The frequency of alterations did not differ across the groups. An exception was the SR-M group wherein increased MCHC peaked with a frequency of 0.80, while in the 
other four groups, the frequency ranged between 0.10 and 0.50. Clinically healthy outdoor dogs have multiple CBC abnormalities, consistent with stress and low-level chronic inflammation, but not associated with a previous exposure to Anaplasma spp. or Babesia spp. The presence of microfilaria increases haemolysis in vitro., Klinički zdravi psi koji borave na otvorenom i koji su izloženi patogenima koje prenose vektori mogu imati hematološke premećaje vezane za dejstvo tih patogena. Ipak, hematološke pretrage se retko izvode na toj populaciji pasa. Stoga je cilj našeg rada bio da utvrdimo promene u krvnoj slici zdravih pasa lutalica, pasa smeštenih u azilima i lovačkih pasa, seroreaktivnih na uzročnike bolesti iz rodova Anaplasma i Babesia, sa ili bez mikrofilarioze. U ovom radu je ispitana krvna slika 81 psa. U studiju su uključeni psi kod kojih lančanom reakcijom polimerizacije nije pokazano prisustvo DNK Anaplasma spp. i Babesia spp. Psi su prema serološkom statusu raspoređeni u sledeće grupe: seronegativni na Anaplasma spp. i Babesia spp. SN (N=26), seroreaktivni na A. phagocytophilum SR-A (N=12), seroreaktivni na B. canis, B. gibsoni i/ili B. vogeli SR-B (N=25) i seroreaktivni na oba navedena patogena SR-AB (N=8). Ove četiri grupe su bile negativne na mikrofilariozu, dok je peta grupa, seroreaktivna na neki ili oba patogena imala i mikrofilarije SR-M (N=10). Učestalost promena kompletne krvne slike među svim ispitivanim psima je bila sledeća: 0,35 za leukocitozu, 0,44 za granulocitozu, 0,28 za anemiju, 0,74 za mikrocitozu, 0,37 za povećanu prosečnu koncentraciju hemoglobina u eritrocitima i 0,33 za trombocitopeniju. Grupe se nisu razlikovale u pogledu učestalosti navedenih promena. Izuzetak je grupa SR-M u kojoj je učestalost povećanja vrednosti za prosečnu koncentraciju hemoglobina u eritrocitima iznosila 0,80. U ostale četiri grupe ta učestalost je iznosila između 0,10 i 0,50. Zaključak je da ispitivani psi imaju višestruke poremećaje krvne slike koji najverovatnije odražavaju stres i nizak stepen hronične inflamacije, ali ne i vezu sa prethodnim kontaktom sa Anaplasma spp. i Babesia spp. Prisustvo mikrofilarija izaziva hemolizu in vitro.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Hаematologic indices in clinically healthy outdoor dogs exposed to vector-borne pathogens, Hematološki parametri kod klinički zdravih pasa koji borave na otvorenom i izloženi su patogenima koje prenose vektori",
volume = "74",
number = "2",
pages = "178-186",
doi = "10.2298/VETGL191216005J"
}

Janjić, F., Milanović, Z., Ilić Božović, A., Andrić, N., Spariosu, K., Ajtić, J., Beletić, A.,& Kovačević-Filipović, M.. (2020). Hаematologic indices in clinically healthy outdoor dogs exposed to vector-borne pathogens. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 74(2), 178-186.
https://doi.org/10.2298/VETGL191216005J

Janjić F, Milanović Z, Ilić Božović A, Andrić N, Spariosu K, Ajtić J, Beletić A, Kovačević-Filipović M. Hаematologic indices in clinically healthy outdoor dogs exposed to vector-borne pathogens. in Veterinarski Glasnik. 2020;74(2):178-186.
doi:10.2298/VETGL191216005J .

Janjić, Filip, Milanović, Zorana, Ilić Božović, Anja, Andrić, Nenad, Spariosu, Kristina, Ajtić, Jelena, Beletić, Anđelo, Kovačević-Filipović, Milica, "Hаematologic indices in clinically healthy outdoor dogs exposed to vector-borne pathogens" in Veterinarski Glasnik, 74, no. 2 (2020):178-186,
https://doi.org/10.2298/VETGL191216005J . .