TY  - GEN
AU  - Vasilev, Dragan
AU  - Glišić, Marija
AU  - Glišić, Milica
AU  - Teodorović, Vlado
AU  - Suvajdžić, Branko
AU  - Vasilev, Saša
AU  - Trbović, Dejana
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3023
AB  - Ovo tehničko rešenje urađeno je u
okviru Projekata: „Unapređenje i razvoj higijenskih i tehnoloških postupaka u proizvodnji
namirnica životinjskog porekla u cilju dobijanja kvalitetnih i bezbednih proizvoda konkurentnih
na svetskom tržištu“ (Ev. broj III46009), rukovodilac projekta prof. dr Mirela Iličić, Tehnološki
fakultet, Univerzitet u Novom Sadu, Novi Sad, kao i projekta „Odabrane biološke opasnosti za
bezbednost/kvalitet hrane animalnog porekla i kontrolne mere od farme do potrošača“ (Ev. broj
TR31034). Rukovodilac projekta Prof. dr Bojan Blagojević, Poljoprivredni fakultet, Univerzitet u
Novom Sadu.
T2  - 2020
T1  - Funkcionalna fermentisana kobasica sa inulinom i lanenim uljem ”Light-Omega”
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3023
ER  - 

@misc{
author = "Vasilev, Dragan and Glišić, Marija and Glišić, Milica and Teodorović, Vlado and Suvajdžić, Branko and Vasilev, Saša and Trbović, Dejana",
abstract = "Ovo tehničko rešenje urađeno je u
okviru Projekata: „Unapređenje i razvoj higijenskih i tehnoloških postupaka u proizvodnji
namirnica životinjskog porekla u cilju dobijanja kvalitetnih i bezbednih proizvoda konkurentnih
na svetskom tržištu“ (Ev. broj III46009), rukovodilac projekta prof. dr Mirela Iličić, Tehnološki
fakultet, Univerzitet u Novom Sadu, Novi Sad, kao i projekta „Odabrane biološke opasnosti za
bezbednost/kvalitet hrane animalnog porekla i kontrolne mere od farme do potrošača“ (Ev. broj
TR31034). Rukovodilac projekta Prof. dr Bojan Blagojević, Poljoprivredni fakultet, Univerzitet u
Novom Sadu.",
journal = "2020",
title = "Funkcionalna fermentisana kobasica sa inulinom i lanenim uljem ”Light-Omega”",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3023"
}

Vasilev, D., Glišić, M., Glišić, M., Teodorović, V., Suvajdžić, B., Vasilev, S.,& Trbović, D..Funkcionalna fermentisana kobasica sa inulinom i lanenim uljem ”Light-Omega”. in 2020.
https://hdl.handle.net/21.15107/rcub_veterinar_3023

Vasilev D, Glišić M, Glišić M, Teodorović V, Suvajdžić B, Vasilev S, Trbović D. Funkcionalna fermentisana kobasica sa inulinom i lanenim uljem ”Light-Omega”. in 2020..
https://hdl.handle.net/21.15107/rcub_veterinar_3023 .

Vasilev, Dragan, Glišić, Marija, Glišić, Milica, Teodorović, Vlado, Suvajdžić, Branko, Vasilev, Saša, Trbović, Dejana, "Funkcionalna fermentisana kobasica sa inulinom i lanenim uljem ”Light-Omega”" in 2020,
https://hdl.handle.net/21.15107/rcub_veterinar_3023 .

TY  - CONF
AU  - Vasilev, Saša
AU  - Suvajdžić, Branko
AU  - Sabljić, Ljiljana
AU  - Mitić, Ivana
AU  - Karabasil, Nedjeljko
AU  - Vejnović, Branislav
AU  - Mirilović, Milorad
AU  - Petrović, Miloš
AU  - Vasilev, Dragan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3080
AB  - U zemljama Evropske unije redovno se sprovode redovne kontrole kvaliteta
pregleda uzoraka mesa na prisustvo larvi Trichinella (PT). U ovim ispitivanjima
učestvuju sve laboratorije u kojima se koristi metod veštačke digestije mesa. Prvu
ovakvu proveru u Srbiji uspešno je organizovao INEP 2017 godine. U narednim
godinama redovno se sprovode ovakve kontrole. Tokom 2022 Fakultet
veterinarske medicine uspešno je organizovao ovakvu kontrolu za laboratorije
veterinarskih specijalističkih instituta, nekih stanica i klanica. Krajem 2022 INEP
je od strane Ministarstva poljoprivrede šumarstva i vodoprivrede, Uprave za
veterinu imenovan kao Nacionalna referentna laboratorija za otkrivanje trihinele
u mesu životinja (NRLT). Tokom 2023 godine NRLT Srbije je organizovala novu
kontrolu kvaliteta rada. Naša iskustva pokazuju da redovno učešće u eksternim
kontrolama kvaliteta pregleda dovodi do postizanja boljih rezultata laboratorija
kroz vreme i bolje zaštite potrošača. Potrebno je da sve laboratorije u Srbiji (koje
se bave pregledom mesa na prisustvo larvi trihinela u mesu) ali i u okruženju
učestvuju u ovakvim proverama najmanje jednom godišnje, kao i vanredno kad
kod imaju novog člana tima. Takođe, poželjno je da sve ove laboratorije imaju
referentni materijal (larve trihinela u alkoholu) i po potrebi zatraže probne uzorke
radi treninga.
AB  - In the European Union countries regular quality controls (PT) are carried out for
the examination of meat samples for the presence of Trichinella larvae. All
laboratories that use the method of artificial digestion of meat participate in these
tests. The first such control in Serbia was successfully organized by INEP in 2017.
In the following years, such controls were carried out regularly. During 2022, the
Faculty of Veterinary Medicine successfully organized control for laboratories
from veterinary specialist institutes, some veterinary stations and
slaughterhouses. At the end of 2022, INEP was appointed by the Ministry of
Agriculture, Forestry and Water Management, Veterinary Directorate as the
National Reference Laboratory for the detection of Trichinella in meat (NRLT).
During 2023, NRLT organized a new quality control. Our experience shows that
regular participation in external quality control leads to better laboratory results
over time and better consumer protection. It is necessary that all laboratories in
Serbia (which control meat for the presence of Trichinella larvae) and in the
surrounding countries participate in such controls at least once a year, as well as
aditionally when they have a new team member. Also, it is desirable that all these
laboratories have a reference material (Trichinella larvae in alcohol) and, if
necessary, request test samples for training purposes.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023
T1  - Kontrola kvaliteta pregleda uzoraka mesa na prisustvo larvi Trichinella – iskustvo iz Srbije
T1  - Quality control of examination of meat samples for the presence of Trichinella larvae - experience from Serbia
SP  - 136
EP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3080
ER  - 

@conference{
author = "Vasilev, Saša and Suvajdžić, Branko and Sabljić, Ljiljana and Mitić, Ivana and Karabasil, Nedjeljko and Vejnović, Branislav and Mirilović, Milorad and Petrović, Miloš and Vasilev, Dragan",
year = "2023",
abstract = "U zemljama Evropske unije redovno se sprovode redovne kontrole kvaliteta
pregleda uzoraka mesa na prisustvo larvi Trichinella (PT). U ovim ispitivanjima
učestvuju sve laboratorije u kojima se koristi metod veštačke digestije mesa. Prvu
ovakvu proveru u Srbiji uspešno je organizovao INEP 2017 godine. U narednim
godinama redovno se sprovode ovakve kontrole. Tokom 2022 Fakultet
veterinarske medicine uspešno je organizovao ovakvu kontrolu za laboratorije
veterinarskih specijalističkih instituta, nekih stanica i klanica. Krajem 2022 INEP
je od strane Ministarstva poljoprivrede šumarstva i vodoprivrede, Uprave za
veterinu imenovan kao Nacionalna referentna laboratorija za otkrivanje trihinele
u mesu životinja (NRLT). Tokom 2023 godine NRLT Srbije je organizovala novu
kontrolu kvaliteta rada. Naša iskustva pokazuju da redovno učešće u eksternim
kontrolama kvaliteta pregleda dovodi do postizanja boljih rezultata laboratorija
kroz vreme i bolje zaštite potrošača. Potrebno je da sve laboratorije u Srbiji (koje
se bave pregledom mesa na prisustvo larvi trihinela u mesu) ali i u okruženju
učestvuju u ovakvim proverama najmanje jednom godišnje, kao i vanredno kad
kod imaju novog člana tima. Takođe, poželjno je da sve ove laboratorije imaju
referentni materijal (larve trihinela u alkoholu) i po potrebi zatraže probne uzorke
radi treninga., In the European Union countries regular quality controls (PT) are carried out for
the examination of meat samples for the presence of Trichinella larvae. All
laboratories that use the method of artificial digestion of meat participate in these
tests. The first such control in Serbia was successfully organized by INEP in 2017.
In the following years, such controls were carried out regularly. During 2022, the
Faculty of Veterinary Medicine successfully organized control for laboratories
from veterinary specialist institutes, some veterinary stations and
slaughterhouses. At the end of 2022, INEP was appointed by the Ministry of
Agriculture, Forestry and Water Management, Veterinary Directorate as the
National Reference Laboratory for the detection of Trichinella in meat (NRLT).
During 2023, NRLT organized a new quality control. Our experience shows that
regular participation in external quality control leads to better laboratory results
over time and better consumer protection. It is necessary that all laboratories in
Serbia (which control meat for the presence of Trichinella larvae) and in the
surrounding countries participate in such controls at least once a year, as well as
aditionally when they have a new team member. Also, it is desirable that all these
laboratories have a reference material (Trichinella larvae in alcohol) and, if
necessary, request test samples for training purposes.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023",
title = "Kontrola kvaliteta pregleda uzoraka mesa na prisustvo larvi Trichinella – iskustvo iz Srbije, Quality control of examination of meat samples for the presence of Trichinella larvae - experience from Serbia",
pages = "136-137",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3080"
}

Vasilev, S., Suvajdžić, B., Sabljić, L., Mitić, I., Karabasil, N., Vejnović, B., Mirilović, M., Petrović, M.,& Vasilev, D.. (2023). Kontrola kvaliteta pregleda uzoraka mesa na prisustvo larvi Trichinella – iskustvo iz Srbije. in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023
Banja Luka : Veterinarska komora Republike Srpske., 136-137.
https://hdl.handle.net/21.15107/rcub_veterinar_3080

Vasilev S, Suvajdžić B, Sabljić L, Mitić I, Karabasil N, Vejnović B, Mirilović M, Petrović M, Vasilev D. Kontrola kvaliteta pregleda uzoraka mesa na prisustvo larvi Trichinella – iskustvo iz Srbije. in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023. 2023;:136-137.
https://hdl.handle.net/21.15107/rcub_veterinar_3080 .

Vasilev, Saša, Suvajdžić, Branko, Sabljić, Ljiljana, Mitić, Ivana, Karabasil, Nedjeljko, Vejnović, Branislav, Mirilović, Milorad, Petrović, Miloš, Vasilev, Dragan, "Kontrola kvaliteta pregleda uzoraka mesa na prisustvo larvi Trichinella – iskustvo iz Srbije" in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023 (2023):136-137,
https://hdl.handle.net/21.15107/rcub_veterinar_3080 .

TY  - CONF
AU  - Vasilev, Saša
AU  - Suvajdžić, Branko
AU  - Mirilović, Milorad
AU  - Ćirović, Duško
AU  - Vejnović, Branislav
AU  - Plavšić, Budimir
AU  - Vasilev, Dragan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3241
AB  - Porast broja ljudske populacije u svetu i nastale promene prirodnog okruženja
povećavaju mogućnost za interakciju između ljudi i životinja, što može dovesti do
pojave i širenja različitih zaraznih bolesti. Bolesti divljih životinja mogu da utiču utiču
osim na same divlje životinje i na javno zdravlje, ekonomiju ali i na očuvanje
biodiverziteta. Bolesti koje se mogu preneti sa divljih i domaćih životinja na čoveka,
koje se označavaju kao zoonoze, mogu biti izazvane određenim parazitima. Parazitske
nematode iz roda Trichinella su uzročnici infekcije kod životinja ali i zoonotske bolesti
trihineloze, koja predstavlja ozbiljan rizik po ljudsko zdravlje. Životinje zaražene
trihinelom, osim u težim slučajevima, ne pokazuju spoljne znake infekcije. Brojne vrste
divljih životinja mogu nositi larve trihinela. Divlje životinje se mogu zaraziti
konzumiranjem drugih inficiranih divljih životinja ili otpadaka koji sadrže zaraženo
meso. Rod Trichinella obuhvata 10 vrsta: T. spiralis, T. nativa, T. britovi, T. murrelli, T.
nelsoni, T. patagoniensis i T. chanchalensis (vrste koje stvaraju kapsulu), T.
pseudospiralis, T. papuae i T. zimbabvensis (neinkapsulirane vrste) kao i tri genotipa
(Trichinella T6, T8 i T9). Ove nematode su rasprostranjene širom sveta i mogu
inficirati širok spektar vrsta životinja, a prenose se u domaćem i silvatičnom ciklusu.
Do 2009. godine T. spiralis se smatrala jedinom vrstom iz roda Trichinella koji je
prisutan kod domaćih i divljih životinja u Srbiji. Tada je primenom molekularne
analize potvrđeno prisustvo T. britovi kod lisica i vukova u Srbiji, pored već
potvrđenog prisustva T. spiralis. Ove analize su bile početak opsežnih ispitivanja
rasprostranjenosti trihinele kod divljih životinja u našoj zemlji. Ustanovljeno je da
postoji infekcija trihinelom kod nekoliko vrsta divljih životinja (vuk, lisica, šakal, divlja
svinja, divlja mačka, jazavac, kuna, mrki tvor, vidra), što omogućava održavanje ovog parazita u silvatičnom krugu ali i eventualno prenošenje Trichinella spp. do svinja koje
se gaje u dvorištima ili puštaju u prirodu. Podaci pokazuju da je prisutna infekcija
trihinelom kod 6% od 469 ispitanih divljih mesojeda prikupljenih u periodu od 1994.
do 2013. godine u Srbiji. Novija istraživanja su pokazala da je polovina (49,5%)
ispitanih vukova u Srbiji zaražena sa T. britovi. Utvrđeno je da je 16,5% zlatnih šakala
bilo zaraženo trihinelom, od kojih je više od dve trećine imalo larve T. spiralis i trećina
T. britovi. Ovakva istraživanja su pokazala da postoji rizik da nekoliko vrsta divljih
životinja može preneti trihinelu na populaciju svinja u slobodnom uzgoju i u dvorištu
i na taj način doprineti održavanju i širenju infekcije.Od četiri vrste trihinele (T.
spiralis, T. britovi, T. pseudospiralis i T. nativa) identifikovanih u Evropi do sada, u Srbiji
kod divljih životinja dokazano je prisustvo dve vrste (T. spiralis i T. britovi). Radi
sprečavanja kruženja trihinele u lovištima neophodno je kao i za sprečavanje drugih
zaraznih bolesti pridržavati se svih biosigurnosnih mera (odgovorni lov, primena
sanitarnih mera tokom svih faza lova, evisceracija ulovljenih životinja na za to
predviđenim mestima i bezbedno odlaganje, nakon uzimanja trofeja od mesojeda
obavezno neškodljivo uklanjanje trupova, postepeno smanjenje štetočina, bolesne
životinje i sve leševe divljih životinja nađenih u lovištu potrebno je odmah prijaviti
nadležnom veterinaru) u lovištu za dobro gazdovanje lovištem. Što se tiče prekidanja
lanca kruženja trihinele izuzetno je važno da lovci ne hrane divlje životinje mesom ili
proizvodima od mesa (kobasicama, nekuvanim mesom, ostacima hrane koji sadrže
meso - pomije) koji nisu pregledani na prisustvo larvi trihinele ili čak sadrže ove larve.
Osim toga važno je napomenuti da u skladu sa propisima uzorak mišića svake
odstreljene divlje svinje mora da bude ispitan na prisustvo larvi trihinela metodom
veštačke digestije. Znacaj ove mere je ne samo zbog otkrivanja pozitivnih divljih svinja
i sprečavanja infekcije ljudi već i zbog neskodljivog uklanjanja pozitivnih i prekidanja
lanca širenja u lovistu.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
T1  - Trihinela kod divljih životinja u Srbiji
SP  - 248
EP  - 249
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3241
ER  - 

@conference{
author = "Vasilev, Saša and Suvajdžić, Branko and Mirilović, Milorad and Ćirović, Duško and Vejnović, Branislav and Plavšić, Budimir and Vasilev, Dragan",
year = "2023",
abstract = "Porast broja ljudske populacije u svetu i nastale promene prirodnog okruženja
povećavaju mogućnost za interakciju između ljudi i životinja, što može dovesti do
pojave i širenja različitih zaraznih bolesti. Bolesti divljih životinja mogu da utiču utiču
osim na same divlje životinje i na javno zdravlje, ekonomiju ali i na očuvanje
biodiverziteta. Bolesti koje se mogu preneti sa divljih i domaćih životinja na čoveka,
koje se označavaju kao zoonoze, mogu biti izazvane određenim parazitima. Parazitske
nematode iz roda Trichinella su uzročnici infekcije kod životinja ali i zoonotske bolesti
trihineloze, koja predstavlja ozbiljan rizik po ljudsko zdravlje. Životinje zaražene
trihinelom, osim u težim slučajevima, ne pokazuju spoljne znake infekcije. Brojne vrste
divljih životinja mogu nositi larve trihinela. Divlje životinje se mogu zaraziti
konzumiranjem drugih inficiranih divljih životinja ili otpadaka koji sadrže zaraženo
meso. Rod Trichinella obuhvata 10 vrsta: T. spiralis, T. nativa, T. britovi, T. murrelli, T.
nelsoni, T. patagoniensis i T. chanchalensis (vrste koje stvaraju kapsulu), T.
pseudospiralis, T. papuae i T. zimbabvensis (neinkapsulirane vrste) kao i tri genotipa
(Trichinella T6, T8 i T9). Ove nematode su rasprostranjene širom sveta i mogu
inficirati širok spektar vrsta životinja, a prenose se u domaćem i silvatičnom ciklusu.
Do 2009. godine T. spiralis se smatrala jedinom vrstom iz roda Trichinella koji je
prisutan kod domaćih i divljih životinja u Srbiji. Tada je primenom molekularne
analize potvrđeno prisustvo T. britovi kod lisica i vukova u Srbiji, pored već
potvrđenog prisustva T. spiralis. Ove analize su bile početak opsežnih ispitivanja
rasprostranjenosti trihinele kod divljih životinja u našoj zemlji. Ustanovljeno je da
postoji infekcija trihinelom kod nekoliko vrsta divljih životinja (vuk, lisica, šakal, divlja
svinja, divlja mačka, jazavac, kuna, mrki tvor, vidra), što omogućava održavanje ovog parazita u silvatičnom krugu ali i eventualno prenošenje Trichinella spp. do svinja koje
se gaje u dvorištima ili puštaju u prirodu. Podaci pokazuju da je prisutna infekcija
trihinelom kod 6% od 469 ispitanih divljih mesojeda prikupljenih u periodu od 1994.
do 2013. godine u Srbiji. Novija istraživanja su pokazala da je polovina (49,5%)
ispitanih vukova u Srbiji zaražena sa T. britovi. Utvrđeno je da je 16,5% zlatnih šakala
bilo zaraženo trihinelom, od kojih je više od dve trećine imalo larve T. spiralis i trećina
T. britovi. Ovakva istraživanja su pokazala da postoji rizik da nekoliko vrsta divljih
životinja može preneti trihinelu na populaciju svinja u slobodnom uzgoju i u dvorištu
i na taj način doprineti održavanju i širenju infekcije.Od četiri vrste trihinele (T.
spiralis, T. britovi, T. pseudospiralis i T. nativa) identifikovanih u Evropi do sada, u Srbiji
kod divljih životinja dokazano je prisustvo dve vrste (T. spiralis i T. britovi). Radi
sprečavanja kruženja trihinele u lovištima neophodno je kao i za sprečavanje drugih
zaraznih bolesti pridržavati se svih biosigurnosnih mera (odgovorni lov, primena
sanitarnih mera tokom svih faza lova, evisceracija ulovljenih životinja na za to
predviđenim mestima i bezbedno odlaganje, nakon uzimanja trofeja od mesojeda
obavezno neškodljivo uklanjanje trupova, postepeno smanjenje štetočina, bolesne
životinje i sve leševe divljih životinja nađenih u lovištu potrebno je odmah prijaviti
nadležnom veterinaru) u lovištu za dobro gazdovanje lovištem. Što se tiče prekidanja
lanca kruženja trihinele izuzetno je važno da lovci ne hrane divlje životinje mesom ili
proizvodima od mesa (kobasicama, nekuvanim mesom, ostacima hrane koji sadrže
meso - pomije) koji nisu pregledani na prisustvo larvi trihinele ili čak sadrže ove larve.
Osim toga važno je napomenuti da u skladu sa propisima uzorak mišića svake
odstreljene divlje svinje mora da bude ispitan na prisustvo larvi trihinela metodom
veštačke digestije. Znacaj ove mere je ne samo zbog otkrivanja pozitivnih divljih svinja
i sprečavanja infekcije ljudi već i zbog neskodljivog uklanjanja pozitivnih i prekidanja
lanca širenja u lovistu.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023",
title = "Trihinela kod divljih životinja u Srbiji",
pages = "248-249",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3241"
}

Vasilev, S., Suvajdžić, B., Mirilović, M., Ćirović, D., Vejnović, B., Plavšić, B.,& Vasilev, D.. (2023). Trihinela kod divljih životinja u Srbiji. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 248-249.
https://hdl.handle.net/21.15107/rcub_veterinar_3241

Vasilev S, Suvajdžić B, Mirilović M, Ćirović D, Vejnović B, Plavšić B, Vasilev D. Trihinela kod divljih životinja u Srbiji. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023. 2023;:248-249.
https://hdl.handle.net/21.15107/rcub_veterinar_3241 .

Vasilev, Saša, Suvajdžić, Branko, Mirilović, Milorad, Ćirović, Duško, Vejnović, Branislav, Plavšić, Budimir, Vasilev, Dragan, "Trihinela kod divljih životinja u Srbiji" in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023 (2023):248-249,
https://hdl.handle.net/21.15107/rcub_veterinar_3241 .

TY  - JOUR
AU  - Vasilev, Saša
AU  - Ciupescu, Laurentiu Mihai
AU  - Lalkovski, Nikolay
AU  - Balic, Davor
AU  - Vasilev, Dragan
AU  - Marucci, Gianluca
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3134
AB  - In some Southeastern European (SEE) countries, like Serbia, Romania, and Bulgaria, trichinellosis is one of the
most important foodborne zoonotic diseases. In those countries, EU regulation and local authorities require the
staff of laboratories performing official controls on meat to be properly trained and to check their competence by
participating regularly in proficiency testing (PT). PTs are organized by National Reference Laboratories for
Trichinella of each country and involve all official laboratories testing meat. In Romania and Bulgaria, the organization
of PT for the detection of Trichinella larvae in meat by Magnetic Stirrer Method (MSM) started in
2012. In Croatia and Serbia PT was first organized in 2015 and 2017, respectively. This study presents data on
the performance obtained by official laboratories of SEE countries that organize PT at national level and compares
the performance obtained by laboratories belonging to different countries. Results suggest that the constant
participation in PT leads to an increase in the performance of participating laboratories by positively affecting
the staff accuracy in sample testing by MSM. Since the percentage of recovered larvae was in some cases suboptimal
(<80%) and occasionally very poor (<40%), there is room for improvement. The regular participation in
PT by laboratories involved in official controls on meat intended for human consumption is fundamental to
guarantee consumer safety.
PB  - Elsevier
T2  - Veterinary Parasitology
T1  - Trichinella proficiency testing in Southeastern European countries
VL  - 320
SP  - 109982
DO  - 10.1016/j.vetpar.2023.109982
ER  - 

@article{
author = "Vasilev, Saša and Ciupescu, Laurentiu Mihai and Lalkovski, Nikolay and Balic, Davor and Vasilev, Dragan and Marucci, Gianluca",
year = "2023",
abstract = "In some Southeastern European (SEE) countries, like Serbia, Romania, and Bulgaria, trichinellosis is one of the
most important foodborne zoonotic diseases. In those countries, EU regulation and local authorities require the
staff of laboratories performing official controls on meat to be properly trained and to check their competence by
participating regularly in proficiency testing (PT). PTs are organized by National Reference Laboratories for
Trichinella of each country and involve all official laboratories testing meat. In Romania and Bulgaria, the organization
of PT for the detection of Trichinella larvae in meat by Magnetic Stirrer Method (MSM) started in
2012. In Croatia and Serbia PT was first organized in 2015 and 2017, respectively. This study presents data on
the performance obtained by official laboratories of SEE countries that organize PT at national level and compares
the performance obtained by laboratories belonging to different countries. Results suggest that the constant
participation in PT leads to an increase in the performance of participating laboratories by positively affecting
the staff accuracy in sample testing by MSM. Since the percentage of recovered larvae was in some cases suboptimal
(<80%) and occasionally very poor (<40%), there is room for improvement. The regular participation in
PT by laboratories involved in official controls on meat intended for human consumption is fundamental to
guarantee consumer safety.",
publisher = "Elsevier",
journal = "Veterinary Parasitology",
title = "Trichinella proficiency testing in Southeastern European countries",
volume = "320",
pages = "109982",
doi = "10.1016/j.vetpar.2023.109982"
}

Vasilev, S., Ciupescu, L. M., Lalkovski, N., Balic, D., Vasilev, D.,& Marucci, G.. (2023). Trichinella proficiency testing in Southeastern European countries. in Veterinary Parasitology
Elsevier., 320, 109982.
https://doi.org/10.1016/j.vetpar.2023.109982

Vasilev S, Ciupescu LM, Lalkovski N, Balic D, Vasilev D, Marucci G. Trichinella proficiency testing in Southeastern European countries. in Veterinary Parasitology. 2023;320:109982.
doi:10.1016/j.vetpar.2023.109982 .

Vasilev, Saša, Ciupescu, Laurentiu Mihai, Lalkovski, Nikolay, Balic, Davor, Vasilev, Dragan, Marucci, Gianluca, "Trichinella proficiency testing in Southeastern European countries" in Veterinary Parasitology, 320 (2023):109982,
https://doi.org/10.1016/j.vetpar.2023.109982 . .

TY  - CONF
AU  - Vasilev, Saša
AU  - Ćupić, Vitomir
AU  - Eoliae, M.
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/594
PB  - Wiley, Hoboken
C3  - Journal of Veterinary Pharmacology and Therapeutics
T1  - The effect of nitro-aspirins (ncx4016 and ncx4040) on the apoptosis of rat neutrophils in vivo
VL  - 32
SP  - 219
EP  - 219
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_594
ER  - 

@conference{
author = "Vasilev, Saša and Ćupić, Vitomir and Eoliae, M.",
year = "2009",
publisher = "Wiley, Hoboken",
journal = "Journal of Veterinary Pharmacology and Therapeutics",
title = "The effect of nitro-aspirins (ncx4016 and ncx4040) on the apoptosis of rat neutrophils in vivo",
volume = "32",
pages = "219-219",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_594"
}

Vasilev, S., Ćupić, V.,& Eoliae, M.. (2009). The effect of nitro-aspirins (ncx4016 and ncx4040) on the apoptosis of rat neutrophils in vivo. in Journal of Veterinary Pharmacology and Therapeutics
Wiley, Hoboken., 32, 219-219.
https://hdl.handle.net/21.15107/rcub_veterinar_594

Vasilev S, Ćupić V, Eoliae M. The effect of nitro-aspirins (ncx4016 and ncx4040) on the apoptosis of rat neutrophils in vivo. in Journal of Veterinary Pharmacology and Therapeutics. 2009;32:219-219.
https://hdl.handle.net/21.15107/rcub_veterinar_594 .

Vasilev, Saša, Ćupić, Vitomir, Eoliae, M., "The effect of nitro-aspirins (ncx4016 and ncx4040) on the apoptosis of rat neutrophils in vivo" in Journal of Veterinary Pharmacology and Therapeutics, 32 (2009):219-219,
https://hdl.handle.net/21.15107/rcub_veterinar_594 .

TY  - CONF
AU  - Vasilev, Saša
AU  - Vučević, Dragana
AU  - Gašić, Sonja
AU  - Vasilijić, Sasa
AU  - Ćupić, Vitomir
AU  - Colić, Miodrag
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/542
PB  - Elsevier Ireland Ltd, Clare
C3  - Toxicology Letters
T1  - The effect of nitro-aspirin (NCX4040) on the apoptosis of neutrophils in vivo
VL  - 180
SP  - S220
EP  - S220
DO  - 10.1016/j.toxlet.2008.06.151
ER  - 

@conference{
author = "Vasilev, Saša and Vučević, Dragana and Gašić, Sonja and Vasilijić, Sasa and Ćupić, Vitomir and Colić, Miodrag",
year = "2008",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Toxicology Letters",
title = "The effect of nitro-aspirin (NCX4040) on the apoptosis of neutrophils in vivo",
volume = "180",
pages = "S220-S220",
doi = "10.1016/j.toxlet.2008.06.151"
}

Vasilev, S., Vučević, D., Gašić, S., Vasilijić, S., Ćupić, V.,& Colić, M.. (2008). The effect of nitro-aspirin (NCX4040) on the apoptosis of neutrophils in vivo. in Toxicology Letters
Elsevier Ireland Ltd, Clare., 180, S220-S220.
https://doi.org/10.1016/j.toxlet.2008.06.151

Vasilev S, Vučević D, Gašić S, Vasilijić S, Ćupić V, Colić M. The effect of nitro-aspirin (NCX4040) on the apoptosis of neutrophils in vivo. in Toxicology Letters. 2008;180:S220-S220.
doi:10.1016/j.toxlet.2008.06.151 .

Vasilev, Saša, Vučević, Dragana, Gašić, Sonja, Vasilijić, Sasa, Ćupić, Vitomir, Colić, Miodrag, "The effect of nitro-aspirin (NCX4040) on the apoptosis of neutrophils in vivo" in Toxicology Letters, 180 (2008):S220-S220,
https://doi.org/10.1016/j.toxlet.2008.06.151 . .

TY  - JOUR
AU  - Vasilev, Saša
AU  - Vučević, Dragana
AU  - Gašić, Sonja
AU  - Majstorović, Ivana
AU  - Vasilijić, S.
AU  - Ćupić, Vitomir
AU  - Čolić, M.
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/557
AB  - Apoptosis of neutrophil granulocytes is a critical event in the resolution of inflammation. Neutrophils have a short lifespan which can be modulated by aspirin. In this work we studied the effect of a nitroaspirin (NCX4040) on apoptosis of inflammatory granulocytes. This nitro-aspirin has been synthesized in attempt to reduce the side effects of aspirin in the gastrointestinal tract. Inflammatory granulocytes have been isolated from polyvinyl sponges implanted under the skin of Albino Oxford (AO) rats. Inflammatory cells that were isolated 20 hours later were about 95% neutrophil granulocytes. The cells were cultivated 24h with different concentrations of NCX4040 ranging from 0.01 μM to 10μM. After that period, apoptosis of neutrophils was performed by using morphological criteria, as well as by flow cytometry (after staining the cells with propidium iodide). We found that NCX4040 at concentrations from 0.25 to 10 μM induced the apoptosis of rat inflammatory granulocytes in a dose-dependent manner. Also, in these concentrations NCX4040 decreased production of nitric oxide in the cells culture supernatants. In conclusion, our results suggest that antiinflammatory properties of NO-aspirins are additionally potentiated by their proapoptotic effect on granulocytes, which could be a novel mechanism of their action.
AB  - Neutrofilni granulociti su deo imunog sistema, uključeni u odbranu organizma od mikroorganizama. Oni imaju najkraći životni vek među leukocitima, koji se može modulisati citokinima i farmakološkim agensima. Efekat novog derivata aspirina, NCX4040, na apoptozu inflamatornih granulocita do sada nije ispitivan. Zbog toga smo u ovom radu ispitivali efekat nitro-aspirina (NCX4040) na apoptozu inflamatornih neutrofilnih granulocita pacova. Inflamatorni granulociti su izolovani iz polivinilskih sunđera implantiranih potkožno pacovima Albino Oxford (AO) soja. Inflamatorne ćelije koje su izolovane 20 sati kasnije, najvećim delom (više od 95 %) su bili neutrofilni granulociti. Ove ćelije su kultivisane 24 sata sa NCX4040 u koncentracijama od 0.01 μM do 10 μM. Supernatanti kultura neutrofila su sakupljani, i korišćeni za merenje koncentracije NO. Ćelije su bojene propidijum jodidom i apoptoza je analizirana na protočnom citofluorimetru, kao i pomoću morfoloških kriterijuma. Ustanovili smo da u koncentracijama od 0.25 μM do 10 μM ovaj nitro-aspirin indukuje, na dozno zavisan način, apoptozu neutrofilnih granulocita. Apoptoza indukovana nitro-aspirinom je bila u pozitivnoj korelaciji sa smanjenom produkcijom NO.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The effect of a new nitro-aspirin on apoptosis of neutrophil granulocytes
T1  - Efekat novog nitro-aspirina na apoptozu neutrofilnih granulocita
VL  - 58
IS  - 5-6
SP  - 449
EP  - 457
DO  - 10.2298/AVB0806449V
ER  - 

@article{
author = "Vasilev, Saša and Vučević, Dragana and Gašić, Sonja and Majstorović, Ivana and Vasilijić, S. and Ćupić, Vitomir and Čolić, M.",
year = "2008",
abstract = "Apoptosis of neutrophil granulocytes is a critical event in the resolution of inflammation. Neutrophils have a short lifespan which can be modulated by aspirin. In this work we studied the effect of a nitroaspirin (NCX4040) on apoptosis of inflammatory granulocytes. This nitro-aspirin has been synthesized in attempt to reduce the side effects of aspirin in the gastrointestinal tract. Inflammatory granulocytes have been isolated from polyvinyl sponges implanted under the skin of Albino Oxford (AO) rats. Inflammatory cells that were isolated 20 hours later were about 95% neutrophil granulocytes. The cells were cultivated 24h with different concentrations of NCX4040 ranging from 0.01 μM to 10μM. After that period, apoptosis of neutrophils was performed by using morphological criteria, as well as by flow cytometry (after staining the cells with propidium iodide). We found that NCX4040 at concentrations from 0.25 to 10 μM induced the apoptosis of rat inflammatory granulocytes in a dose-dependent manner. Also, in these concentrations NCX4040 decreased production of nitric oxide in the cells culture supernatants. In conclusion, our results suggest that antiinflammatory properties of NO-aspirins are additionally potentiated by their proapoptotic effect on granulocytes, which could be a novel mechanism of their action., Neutrofilni granulociti su deo imunog sistema, uključeni u odbranu organizma od mikroorganizama. Oni imaju najkraći životni vek među leukocitima, koji se može modulisati citokinima i farmakološkim agensima. Efekat novog derivata aspirina, NCX4040, na apoptozu inflamatornih granulocita do sada nije ispitivan. Zbog toga smo u ovom radu ispitivali efekat nitro-aspirina (NCX4040) na apoptozu inflamatornih neutrofilnih granulocita pacova. Inflamatorni granulociti su izolovani iz polivinilskih sunđera implantiranih potkožno pacovima Albino Oxford (AO) soja. Inflamatorne ćelije koje su izolovane 20 sati kasnije, najvećim delom (više od 95 %) su bili neutrofilni granulociti. Ove ćelije su kultivisane 24 sata sa NCX4040 u koncentracijama od 0.01 μM do 10 μM. Supernatanti kultura neutrofila su sakupljani, i korišćeni za merenje koncentracije NO. Ćelije su bojene propidijum jodidom i apoptoza je analizirana na protočnom citofluorimetru, kao i pomoću morfoloških kriterijuma. Ustanovili smo da u koncentracijama od 0.25 μM do 10 μM ovaj nitro-aspirin indukuje, na dozno zavisan način, apoptozu neutrofilnih granulocita. Apoptoza indukovana nitro-aspirinom je bila u pozitivnoj korelaciji sa smanjenom produkcijom NO.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The effect of a new nitro-aspirin on apoptosis of neutrophil granulocytes, Efekat novog nitro-aspirina na apoptozu neutrofilnih granulocita",
volume = "58",
number = "5-6",
pages = "449-457",
doi = "10.2298/AVB0806449V"
}

Vasilev, S., Vučević, D., Gašić, S., Majstorović, I., Vasilijić, S., Ćupić, V.,& Čolić, M.. (2008). The effect of a new nitro-aspirin on apoptosis of neutrophil granulocytes. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 58(5-6), 449-457.
https://doi.org/10.2298/AVB0806449V

Vasilev S, Vučević D, Gašić S, Majstorović I, Vasilijić S, Ćupić V, Čolić M. The effect of a new nitro-aspirin on apoptosis of neutrophil granulocytes. in Acta Veterinaria-Beograd. 2008;58(5-6):449-457.
doi:10.2298/AVB0806449V .

Vasilev, Saša, Vučević, Dragana, Gašić, Sonja, Majstorović, Ivana, Vasilijić, S., Ćupić, Vitomir, Čolić, M., "The effect of a new nitro-aspirin on apoptosis of neutrophil granulocytes" in Acta Veterinaria-Beograd, 58, no. 5-6 (2008):449-457,
https://doi.org/10.2298/AVB0806449V . .

TY  - JOUR
AU  - Vasilev, Saša
AU  - Vučević, Dragana
AU  - Gašić, Sonja
AU  - Majstorović, Ivana
AU  - Vasilijić, S.
AU  - Čolić, M.
AU  - Ćupić, Vitomir
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/463
AB  - Apoptosis of neutrophil granulocytes is a critical event in the resolution of inflammation. Neutrophils have a short lifespan which can be modulated by aspirin. In this work we studied the effect of a nitroaspirin (NCX4016) on apoptosis of inflammatory granulocytes. This aspirin analogue has been synthesized to reduce the side effects of aspirin in the gastrointestinal tract. Inflammatory granulocytes have been isolated from polyvinyl sponges implanted under the skin of Albino Oxford (AO) rats. Inflammatory cells that were isolated 20 hours later were about 95% neutrophil granulocytes. The cells were cultivated 24h with different concentrations of NCX4016 ranging from 0.25 μM to 500 μM. After that period, apoptosis of neutrophils was assessed by morphological criteria, as well as by flow cytometry (after staining the cells with propidium iodide). We found that NCX4016 at concentrations from 50 to 500 μM induced the apoptosis of rat inflammatory granulocytes in a dose-dependent manner. However, at concentrations from 1 μM to 10 μM it inhibited apoptosis. In conclusion, our results suggest that anti-inflammatory properties of the NO-aspirin are additionally potentiated by the proapoptotic effect on granulocytes, which could be a novel mechanism of their action.
AB  - Neutrofilni granulociti imaju najkraći životni vek među leukocitima, koji se može modulisati citokinima i farmakološkim agensima. Efekat nitro-aspirina na apoptozu inflamatornih granulocita do sada nije ispitivan. Zbog toga smo u ovom radu ispitivali efekat nitro-aspirina (NCX-4016) na apoptozu inflamatornih neutrofilnih granulocita pacova. Inflamatorni granulociti su izolovani iz polivinilskih sunđera potkožno implantiranih pacovima Albino Oxford (AO) soja. Inflamatorne ćelije koje su izolovane 20 sati kasnije, najvećim delom (više od 95 %) su bili neutrofilni granulociti. Ove ćelije su kultivisane 24 sata sa NCX-4016 u koncentracijama od 0,25 μM do 500 μM. Supernatanti kultura su sakupljani i korišćeni za merenje koncentracije NO. Ćelije su bojene propidijum jodidom i apoptoza je analizirana na protočnom citofluorimetru, kao i pomoću morfoloških kriterijuma. Ustanovljeno je da u koncentracijama od 1 μM do 10 μM nitro-aspirin inhibiše, a u koncentracijama višim od 50 μM indukuje apoptozu ovih ćelija, na dozno zavistan način. Nitro-aspirinom indukovana apoptoza je u pozitivnoj korelaciji sa smanjenom produkcijom NO.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The effect of a nitro-aspirine on apoptosis of neutrophil granulocytes
T1  - Efekat nitro-aspirina na apoptozu neutrofilnih granulocita
VL  - 57
IS  - 5-6
SP  - 403
EP  - 412
DO  - 10.2298/AVB0706403V
ER  - 

@article{
author = "Vasilev, Saša and Vučević, Dragana and Gašić, Sonja and Majstorović, Ivana and Vasilijić, S. and Čolić, M. and Ćupić, Vitomir",
year = "2007",
abstract = "Apoptosis of neutrophil granulocytes is a critical event in the resolution of inflammation. Neutrophils have a short lifespan which can be modulated by aspirin. In this work we studied the effect of a nitroaspirin (NCX4016) on apoptosis of inflammatory granulocytes. This aspirin analogue has been synthesized to reduce the side effects of aspirin in the gastrointestinal tract. Inflammatory granulocytes have been isolated from polyvinyl sponges implanted under the skin of Albino Oxford (AO) rats. Inflammatory cells that were isolated 20 hours later were about 95% neutrophil granulocytes. The cells were cultivated 24h with different concentrations of NCX4016 ranging from 0.25 μM to 500 μM. After that period, apoptosis of neutrophils was assessed by morphological criteria, as well as by flow cytometry (after staining the cells with propidium iodide). We found that NCX4016 at concentrations from 50 to 500 μM induced the apoptosis of rat inflammatory granulocytes in a dose-dependent manner. However, at concentrations from 1 μM to 10 μM it inhibited apoptosis. In conclusion, our results suggest that anti-inflammatory properties of the NO-aspirin are additionally potentiated by the proapoptotic effect on granulocytes, which could be a novel mechanism of their action., Neutrofilni granulociti imaju najkraći životni vek među leukocitima, koji se može modulisati citokinima i farmakološkim agensima. Efekat nitro-aspirina na apoptozu inflamatornih granulocita do sada nije ispitivan. Zbog toga smo u ovom radu ispitivali efekat nitro-aspirina (NCX-4016) na apoptozu inflamatornih neutrofilnih granulocita pacova. Inflamatorni granulociti su izolovani iz polivinilskih sunđera potkožno implantiranih pacovima Albino Oxford (AO) soja. Inflamatorne ćelije koje su izolovane 20 sati kasnije, najvećim delom (više od 95 %) su bili neutrofilni granulociti. Ove ćelije su kultivisane 24 sata sa NCX-4016 u koncentracijama od 0,25 μM do 500 μM. Supernatanti kultura su sakupljani i korišćeni za merenje koncentracije NO. Ćelije su bojene propidijum jodidom i apoptoza je analizirana na protočnom citofluorimetru, kao i pomoću morfoloških kriterijuma. Ustanovljeno je da u koncentracijama od 1 μM do 10 μM nitro-aspirin inhibiše, a u koncentracijama višim od 50 μM indukuje apoptozu ovih ćelija, na dozno zavistan način. Nitro-aspirinom indukovana apoptoza je u pozitivnoj korelaciji sa smanjenom produkcijom NO.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The effect of a nitro-aspirine on apoptosis of neutrophil granulocytes, Efekat nitro-aspirina na apoptozu neutrofilnih granulocita",
volume = "57",
number = "5-6",
pages = "403-412",
doi = "10.2298/AVB0706403V"
}

Vasilev, S., Vučević, D., Gašić, S., Majstorović, I., Vasilijić, S., Čolić, M.,& Ćupić, V.. (2007). The effect of a nitro-aspirine on apoptosis of neutrophil granulocytes. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(5-6), 403-412.
https://doi.org/10.2298/AVB0706403V

Vasilev S, Vučević D, Gašić S, Majstorović I, Vasilijić S, Čolić M, Ćupić V. The effect of a nitro-aspirine on apoptosis of neutrophil granulocytes. in Acta Veterinaria-Beograd. 2007;57(5-6):403-412.
doi:10.2298/AVB0706403V .

Vasilev, Saša, Vučević, Dragana, Gašić, Sonja, Majstorović, Ivana, Vasilijić, S., Čolić, M., Ćupić, Vitomir, "The effect of a nitro-aspirine on apoptosis of neutrophil granulocytes" in Acta Veterinaria-Beograd, 57, no. 5-6 (2007):403-412,
https://doi.org/10.2298/AVB0706403V . .

TY  - JOUR
AU  - Vasilev, Saša
AU  - Majstorović, Ivana
AU  - Gašić, Sonja
AU  - Vučević, Dragana
AU  - Vasilijić, S.
AU  - Ćupić, Vitomir
AU  - Čolić, M.
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/422
AB  - Neutrophils are a part of the immune system, and they are involved in host defence against microorganisms. Neutrophil granulocytes have the shortest lifespan among leukocytes, which can be modulated by cytokines and pharmacological agents. The effect of aspirin on apoptosis of inflammatory granulocytes has not been studied in detail yet, and therefore was the chosen subject of this study. Inflammatory granulocytes have been isolated from polyvinyl sponges implanted under the skin of Albino Oxford (AO) rats. Inflammatory cells that were isolated 20 hours later were more than 95% neutrophil granulocytes. The cells were cultivated 24 h with different concentrations of aspirin ranging from 1 µM to 10 mM. After the cultivation period, apoptosis of neutrophils was assessed by morphological criteria, as well as by flow cytometry (after staining the cells with propidium iodide). We found that at concentrations from 0,1 mM to 2,5 mM aspirin inhibited apoptosis of granulocytes, but at 10 mM aspirin induced apoptosis of these cells.
AB  - Neutrofilni granulociti su deo imunog sistema, uključenog u odbranu od mikroorganizama. Oni imaju najkraći životni vek među leukocitima, koji se može modulisati citokinima i farmakološkim agensima, a do sada nije ispitivan efekat aspirina na apoptozu inflamatornih granulocita. Zbog toga je u ovoj studiji ispitivan efekat aspirina na apoptozu inflamatornih neutrofilnih granulocita pacova. Inflamatorni granulociti su izolovani iz polivinilskih sunđera, potkožno implantiranih, pacovima Albino Oxford (AO) soja. Inflamatorne ćelije, izolovane 20 sati kasnije, najvećim delom (više od 95 %) predstavljaju neutrofilne granulocite. Ove ćelije su kultivisane 24 sata sa aspirinom u koncentracijama od 1 µM do 10 mM. Posle ovog perioda supernatanti su sakupljani i korišćeni za merenje koncentracije NO. Ćelije su bojene propidijum jodidom i apoptoza je analizirana na protočnom citofluorimetru, kao i pomoću morfoloških kriterijuma. Ustanovljeno je da u koncentracijama od 0,1 do 2,5 mM aspirin inhibira, a samo u visokim koncentracijama (10 mM) indukuje apoptozu ovih ćelija. Aspirinom indukovana apoptoza je bila u pozitivnoj korelaciji sa smanjenom produkcijom NO.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The effects of aspirin on apoptosis of neutrophil granulocytes
T1  - Efekti aspirina na apoptozu neutrofilnih granulocita
VL  - 56
IS  - 5-6
SP  - 413
EP  - 421
DO  - 10.2298/AVB0606413V
ER  - 

@article{
author = "Vasilev, Saša and Majstorović, Ivana and Gašić, Sonja and Vučević, Dragana and Vasilijić, S. and Ćupić, Vitomir and Čolić, M.",
year = "2006",
abstract = "Neutrophils are a part of the immune system, and they are involved in host defence against microorganisms. Neutrophil granulocytes have the shortest lifespan among leukocytes, which can be modulated by cytokines and pharmacological agents. The effect of aspirin on apoptosis of inflammatory granulocytes has not been studied in detail yet, and therefore was the chosen subject of this study. Inflammatory granulocytes have been isolated from polyvinyl sponges implanted under the skin of Albino Oxford (AO) rats. Inflammatory cells that were isolated 20 hours later were more than 95% neutrophil granulocytes. The cells were cultivated 24 h with different concentrations of aspirin ranging from 1 µM to 10 mM. After the cultivation period, apoptosis of neutrophils was assessed by morphological criteria, as well as by flow cytometry (after staining the cells with propidium iodide). We found that at concentrations from 0,1 mM to 2,5 mM aspirin inhibited apoptosis of granulocytes, but at 10 mM aspirin induced apoptosis of these cells., Neutrofilni granulociti su deo imunog sistema, uključenog u odbranu od mikroorganizama. Oni imaju najkraći životni vek među leukocitima, koji se može modulisati citokinima i farmakološkim agensima, a do sada nije ispitivan efekat aspirina na apoptozu inflamatornih granulocita. Zbog toga je u ovoj studiji ispitivan efekat aspirina na apoptozu inflamatornih neutrofilnih granulocita pacova. Inflamatorni granulociti su izolovani iz polivinilskih sunđera, potkožno implantiranih, pacovima Albino Oxford (AO) soja. Inflamatorne ćelije, izolovane 20 sati kasnije, najvećim delom (više od 95 %) predstavljaju neutrofilne granulocite. Ove ćelije su kultivisane 24 sata sa aspirinom u koncentracijama od 1 µM do 10 mM. Posle ovog perioda supernatanti su sakupljani i korišćeni za merenje koncentracije NO. Ćelije su bojene propidijum jodidom i apoptoza je analizirana na protočnom citofluorimetru, kao i pomoću morfoloških kriterijuma. Ustanovljeno je da u koncentracijama od 0,1 do 2,5 mM aspirin inhibira, a samo u visokim koncentracijama (10 mM) indukuje apoptozu ovih ćelija. Aspirinom indukovana apoptoza je bila u pozitivnoj korelaciji sa smanjenom produkcijom NO.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The effects of aspirin on apoptosis of neutrophil granulocytes, Efekti aspirina na apoptozu neutrofilnih granulocita",
volume = "56",
number = "5-6",
pages = "413-421",
doi = "10.2298/AVB0606413V"
}

Vasilev, S., Majstorović, I., Gašić, S., Vučević, D., Vasilijić, S., Ćupić, V.,& Čolić, M.. (2006). The effects of aspirin on apoptosis of neutrophil granulocytes. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(5-6), 413-421.
https://doi.org/10.2298/AVB0606413V

Vasilev S, Majstorović I, Gašić S, Vučević D, Vasilijić S, Ćupić V, Čolić M. The effects of aspirin on apoptosis of neutrophil granulocytes. in Acta Veterinaria-Beograd. 2006;56(5-6):413-421.
doi:10.2298/AVB0606413V .

Vasilev, Saša, Majstorović, Ivana, Gašić, Sonja, Vučević, Dragana, Vasilijić, S., Ćupić, Vitomir, Čolić, M., "The effects of aspirin on apoptosis of neutrophil granulocytes" in Acta Veterinaria-Beograd, 56, no. 5-6 (2006):413-421,
https://doi.org/10.2298/AVB0606413V . .

TY  - CONF
AU  - Vasilev, Saša
AU  - Majstorović, Ivana
AU  - Vučević, Dragana
AU  - Gašić, Sonja
AU  - Vasilijić, S.
AU  - Bufan, Biljana
AU  - Ćupić, Vitomir
AU  - Colić, M.
PY  - 2005
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/333
PB  - Elsevier Ireland Ltd, Clare
C3  - Toxicology Letters
T1  - The effect of NCX 4016 and NCX 4040, two nitric oxide-donating aspirin derivatives, on apoptosis of neutrophil granulocytes in vitro
VL  - 158
SP  - S229
EP  - S229
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_333
ER  - 

@conference{
author = "Vasilev, Saša and Majstorović, Ivana and Vučević, Dragana and Gašić, Sonja and Vasilijić, S. and Bufan, Biljana and Ćupić, Vitomir and Colić, M.",
year = "2005",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Toxicology Letters",
title = "The effect of NCX 4016 and NCX 4040, two nitric oxide-donating aspirin derivatives, on apoptosis of neutrophil granulocytes in vitro",
volume = "158",
pages = "S229-S229",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_333"
}

Vasilev, S., Majstorović, I., Vučević, D., Gašić, S., Vasilijić, S., Bufan, B., Ćupić, V.,& Colić, M.. (2005). The effect of NCX 4016 and NCX 4040, two nitric oxide-donating aspirin derivatives, on apoptosis of neutrophil granulocytes in vitro. in Toxicology Letters
Elsevier Ireland Ltd, Clare., 158, S229-S229.
https://hdl.handle.net/21.15107/rcub_veterinar_333

Vasilev S, Majstorović I, Vučević D, Gašić S, Vasilijić S, Bufan B, Ćupić V, Colić M. The effect of NCX 4016 and NCX 4040, two nitric oxide-donating aspirin derivatives, on apoptosis of neutrophil granulocytes in vitro. in Toxicology Letters. 2005;158:S229-S229.
https://hdl.handle.net/21.15107/rcub_veterinar_333 .

Vasilev, Saša, Majstorović, Ivana, Vučević, Dragana, Gašić, Sonja, Vasilijić, S., Bufan, Biljana, Ćupić, Vitomir, Colić, M., "The effect of NCX 4016 and NCX 4040, two nitric oxide-donating aspirin derivatives, on apoptosis of neutrophil granulocytes in vitro" in Toxicology Letters, 158 (2005):S229-S229,
https://hdl.handle.net/21.15107/rcub_veterinar_333 .