TY  - JOUR
AU  - Nišavić, Jakov
AU  - Milić, Nenad
AU  - Radalj, Andrea
AU  - Mirilović, Milorad
AU  - Vejnović, Branislav
AU  - Ćosić, Milivoje
AU  - Knežević, Aleksandra
AU  - Veljović, Ljubiša
AU  - Živulj, Aleksandar
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2357
AB  - The objective was to expand and update the knowledge on the presence and genotype diversity of porcine circoviruses 2 and 3 (PCV2 and PCV3) in the wild boar populations from the hunting grounds in northeastern Serbia. The presence of PCV3 was not determined, and PCV2 was confirmed in 40.32% of the organ samples from 124 wild boars hunted from 2018 to 2019, indicating their significance in virus circulation since traditional pig farms with irregular PCV2 vaccination strategies are widespread in this region. The most prevalent genotype was PCV2d, followed by PCV2b and PCV2a in 55.6%, 38.9%, and 5.5% of the examined samples, respectively. Nucleotide sequences of the detected strains were homogenous within the genotype and clustered within the subgroups PCV2d-2, PCV2b-1A/B, and PCV2a-2D with high identity to European, Chinese, and Serbian domestic pig sequences suggesting their origin. Wild boars presented with no clinical or pathological signs of infection, implying that these animals might be less susceptible to disease, particularly since the cofactors present in pig farming systems that support the disease development are absent in the wild. The high PCV2 detection frequency demonstrates the importance of wildlife monitoring to track virus population dynamics, especially in regions with free-range pig farming in order to plan adequate disease control strategies.
T2  - Veterinarni Medicina
T1  - Detection and characterisation of porcine circoviruses in wild boars in northeastern Serbia
VL  - 67
DO  - 10.17221/32/2021-VETMED
ER  - 

@article{
author = "Nišavić, Jakov and Milić, Nenad and Radalj, Andrea and Mirilović, Milorad and Vejnović, Branislav and Ćosić, Milivoje and Knežević, Aleksandra and Veljović, Ljubiša and Živulj, Aleksandar",
year = "2022",
abstract = "The objective was to expand and update the knowledge on the presence and genotype diversity of porcine circoviruses 2 and 3 (PCV2 and PCV3) in the wild boar populations from the hunting grounds in northeastern Serbia. The presence of PCV3 was not determined, and PCV2 was confirmed in 40.32% of the organ samples from 124 wild boars hunted from 2018 to 2019, indicating their significance in virus circulation since traditional pig farms with irregular PCV2 vaccination strategies are widespread in this region. The most prevalent genotype was PCV2d, followed by PCV2b and PCV2a in 55.6%, 38.9%, and 5.5% of the examined samples, respectively. Nucleotide sequences of the detected strains were homogenous within the genotype and clustered within the subgroups PCV2d-2, PCV2b-1A/B, and PCV2a-2D with high identity to European, Chinese, and Serbian domestic pig sequences suggesting their origin. Wild boars presented with no clinical or pathological signs of infection, implying that these animals might be less susceptible to disease, particularly since the cofactors present in pig farming systems that support the disease development are absent in the wild. The high PCV2 detection frequency demonstrates the importance of wildlife monitoring to track virus population dynamics, especially in regions with free-range pig farming in order to plan adequate disease control strategies.",
journal = "Veterinarni Medicina",
title = "Detection and characterisation of porcine circoviruses in wild boars in northeastern Serbia",
volume = "67",
doi = "10.17221/32/2021-VETMED"
}

Nišavić, J., Milić, N., Radalj, A., Mirilović, M., Vejnović, B., Ćosić, M., Knežević, A., Veljović, L.,& Živulj, A.. (2022). Detection and characterisation of porcine circoviruses in wild boars in northeastern Serbia. in Veterinarni Medicina, 67.
https://doi.org/10.17221/32/2021-VETMED

Nišavić J, Milić N, Radalj A, Mirilović M, Vejnović B, Ćosić M, Knežević A, Veljović L, Živulj A. Detection and characterisation of porcine circoviruses in wild boars in northeastern Serbia. in Veterinarni Medicina. 2022;67.
doi:10.17221/32/2021-VETMED .

Nišavić, Jakov, Milić, Nenad, Radalj, Andrea, Mirilović, Milorad, Vejnović, Branislav, Ćosić, Milivoje, Knežević, Aleksandra, Veljović, Ljubiša, Živulj, Aleksandar, "Detection and characterisation of porcine circoviruses in wild boars in northeastern Serbia" in Veterinarni Medicina, 67 (2022),
https://doi.org/10.17221/32/2021-VETMED . .

TY  - JOUR
AU  - Nišavić, Jakov
AU  - Milić, Nenad
AU  - Radalj, Andrea
AU  - Krnjaić, Dejan
AU  - Milićević, Dragan
AU  - Knežević, Aleksandra
AU  - Radojičić, Marina
AU  - Obrenović, Sonja
AU  - Ćosić, Milivoje
AU  - Tešović, Bojana
AU  - Benković, Damir
AU  - Živulj Aleksandar
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2044
AB  - Porcine parvoviruses (PPVs) are diverse and persistently evolving viruses found in domestic pigs and wild boars. Porcine parvovirus 1 (PPV1) causes reproductive problems in adult animals, although the veterinary relevance of PPV2, PPV3, and PPV4 has not been clarified. The detection and sequence analysis of PPVs circulating in wild boar populations in Serbia was performed to determine their phylogenetic relationships and prevalence in 122 organ samples collected during 2018. The DNA of PPV1, PPV2, and PPV3 was detected in 56.6% of the examined samples, whilst PPV4 was not identified. Overall, PPV3 was the most prevalent in 69.6% of the positive samples, followed by PPV1 in 63.8%, and PPV2 in 21.7% samples. Single infections were more common, although concurrent infections were confirmed in 34.8% samples for two, and 10.1% samples for three viruses. Sequence analysis of wild boar PPV1 showed no significant nucleotide differences from domestic pig PPV1 strains detected in Europe and the USA, however separate clustering from strains from China and the NADL-2 strain was demonstrated. Examination of the selected PPV2 sequences might suggest a certain geographical distribution of genetically diverse PPV2 strains considering high similarities to the strains from neighboring countries, and variability in comparison with other reported PPV2 sequences from different parts of the world. Wild boar PPV3 sequences clustered separately from most of the strains detected in wild boars, as well as the original porcine hokovirus strain. It is further noted that genetically different PPV3 strains circulate amongst Serbian domestic pigs and wild boars.
AB  - Parvovirusi svinja predstavljaju genetski različite viruse koji izazivaju infekcije doma-ćih i divljih svinja. Parvovirus svinja 1 (PPV1) dovodi do pojave reproduktivnih pro-blema  kod  odraslih  jedinki,  dok  klinički  značaj  PPV2,  PPV3  i  PPV4  još  uvek  nije  u  potpunosti  razjašnjen.  Izvršena  je  detekcija  i  analiza  genetskih  sekvenci  parvovirusa  koji  cirkulišu  u  populaciji  divljih  svinja  u  Srbiji  u  cilju  njihove  fi  logenetske  analize  i  određivanja zastupljenosti u ukupno 122 uzorka organa prikupljenih tokom 2018. go-dine. Prisustvo DNK PPV1, PPV2 i PPV3 detektovano je u 56,6% ispitanih uzoraka, pri čemu  prisustvo  PPV4  nije  utvrđeno.  Među  pozitivnim  uzorcima,  PPV3  je  pro-centualno najzastupljeniji virus detektovan u 69,6%, dok je prisustvo  PPV1 i PPV2 utvrđeno u 63,8%, odnosno u 21,7% pozitivnih uzoraka. Infekcije jednim virusom su češće identifi kovane, međutim, prisustvo mešovitih infekcija sa dva, odnosno tri par-vovirusa zabeleženo je u 34,8% i 10,1% uzoraka. Analizom genetskih sekvenci PPV1 detektovanih kod divljih svinja nisu utvrđene značajnije razlike u odnosu na analogne sekvence PPV1 poreklom od domaćih svinja iz Evrope i SAD, međutim zabeleženo je izdvajanje u zaseban klaster u odnosu na kineske sojeve virusa i soj NADL-2. Ispi-tivanjem sekvenci PPV2 utvrđena je izvesna geografska distribucija genetski različitih sojeva navedenog virusa s obzirom na njihovu veliku sličnost sa sojevima virusa iz su-sednih zemalja. Sekvence PPV3 detektovanih kod divljih svinja su se na fi logenetskom stablu izdvajale u zaseban klaster u odnosu na većinu dostupnih sekvenci navedenog virusa detektovanih kod divljih svinja. Pored toga, zabeleženo je da genetski različiti sojevi PPV3 cirkulišu u populacijama divljih i domaćih svinja u Srbiji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in Serbia
T1  - Genetska analiza i distribucija parvovirusa (ppvs) detektovanih u organima divljih svinja u Srbiji
VL  - 71
IS  - 1
SP  - 32
EP  - 46
DO  - 10.2478/acve-2021-0003
ER  - 

@article{
author = "Nišavić, Jakov and Milić, Nenad and Radalj, Andrea and Krnjaić, Dejan and Milićević, Dragan and Knežević, Aleksandra and Radojičić, Marina and Obrenović, Sonja and Ćosić, Milivoje and Tešović, Bojana and Benković, Damir and Živulj Aleksandar",
year = "2021",
abstract = "Porcine parvoviruses (PPVs) are diverse and persistently evolving viruses found in domestic pigs and wild boars. Porcine parvovirus 1 (PPV1) causes reproductive problems in adult animals, although the veterinary relevance of PPV2, PPV3, and PPV4 has not been clarified. The detection and sequence analysis of PPVs circulating in wild boar populations in Serbia was performed to determine their phylogenetic relationships and prevalence in 122 organ samples collected during 2018. The DNA of PPV1, PPV2, and PPV3 was detected in 56.6% of the examined samples, whilst PPV4 was not identified. Overall, PPV3 was the most prevalent in 69.6% of the positive samples, followed by PPV1 in 63.8%, and PPV2 in 21.7% samples. Single infections were more common, although concurrent infections were confirmed in 34.8% samples for two, and 10.1% samples for three viruses. Sequence analysis of wild boar PPV1 showed no significant nucleotide differences from domestic pig PPV1 strains detected in Europe and the USA, however separate clustering from strains from China and the NADL-2 strain was demonstrated. Examination of the selected PPV2 sequences might suggest a certain geographical distribution of genetically diverse PPV2 strains considering high similarities to the strains from neighboring countries, and variability in comparison with other reported PPV2 sequences from different parts of the world. Wild boar PPV3 sequences clustered separately from most of the strains detected in wild boars, as well as the original porcine hokovirus strain. It is further noted that genetically different PPV3 strains circulate amongst Serbian domestic pigs and wild boars., Parvovirusi svinja predstavljaju genetski različite viruse koji izazivaju infekcije doma-ćih i divljih svinja. Parvovirus svinja 1 (PPV1) dovodi do pojave reproduktivnih pro-blema  kod  odraslih  jedinki,  dok  klinički  značaj  PPV2,  PPV3  i  PPV4  još  uvek  nije  u  potpunosti  razjašnjen.  Izvršena  je  detekcija  i  analiza  genetskih  sekvenci  parvovirusa  koji  cirkulišu  u  populaciji  divljih  svinja  u  Srbiji  u  cilju  njihove  fi  logenetske  analize  i  određivanja zastupljenosti u ukupno 122 uzorka organa prikupljenih tokom 2018. go-dine. Prisustvo DNK PPV1, PPV2 i PPV3 detektovano je u 56,6% ispitanih uzoraka, pri čemu  prisustvo  PPV4  nije  utvrđeno.  Među  pozitivnim  uzorcima,  PPV3  je  pro-centualno najzastupljeniji virus detektovan u 69,6%, dok je prisustvo  PPV1 i PPV2 utvrđeno u 63,8%, odnosno u 21,7% pozitivnih uzoraka. Infekcije jednim virusom su češće identifi kovane, međutim, prisustvo mešovitih infekcija sa dva, odnosno tri par-vovirusa zabeleženo je u 34,8% i 10,1% uzoraka. Analizom genetskih sekvenci PPV1 detektovanih kod divljih svinja nisu utvrđene značajnije razlike u odnosu na analogne sekvence PPV1 poreklom od domaćih svinja iz Evrope i SAD, međutim zabeleženo je izdvajanje u zaseban klaster u odnosu na kineske sojeve virusa i soj NADL-2. Ispi-tivanjem sekvenci PPV2 utvrđena je izvesna geografska distribucija genetski različitih sojeva navedenog virusa s obzirom na njihovu veliku sličnost sa sojevima virusa iz su-sednih zemalja. Sekvence PPV3 detektovanih kod divljih svinja su se na fi logenetskom stablu izdvajale u zaseban klaster u odnosu na većinu dostupnih sekvenci navedenog virusa detektovanih kod divljih svinja. Pored toga, zabeleženo je da genetski različiti sojevi PPV3 cirkulišu u populacijama divljih i domaćih svinja u Srbiji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in Serbia, Genetska analiza i distribucija parvovirusa (ppvs) detektovanih u organima divljih svinja u Srbiji",
volume = "71",
number = "1",
pages = "32-46",
doi = "10.2478/acve-2021-0003"
}

Nišavić, J., Milić, N., Radalj, A., Krnjaić, D., Milićević, D., Knežević, A., Radojičić, M., Obrenović, S., Ćosić, M., Tešović, B., Benković, D.,& Živulj Aleksandar. (2021). Genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(1), 32-46.
https://doi.org/10.2478/acve-2021-0003

Nišavić J, Milić N, Radalj A, Krnjaić D, Milićević D, Knežević A, Radojičić M, Obrenović S, Ćosić M, Tešović B, Benković D, Živulj Aleksandar. Genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in Serbia. in Acta Veterinaria-Beograd. 2021;71(1):32-46.
doi:10.2478/acve-2021-0003 .

Nišavić, Jakov, Milić, Nenad, Radalj, Andrea, Krnjaić, Dejan, Milićević, Dragan, Knežević, Aleksandra, Radojičić, Marina, Obrenović, Sonja, Ćosić, Milivoje, Tešović, Bojana, Benković, Damir, Živulj Aleksandar, "Genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in Serbia" in Acta Veterinaria-Beograd, 71, no. 1 (2021):32-46,
https://doi.org/10.2478/acve-2021-0003 . .

TY  - JOUR
AU  - Nišavić, Jakov
AU  - Knežević, Aleksandra
AU  - Stanojević, M.
AU  - Milić, Nenad
AU  - Radalj, Andrea
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1645
AB  - The presence of bovine herpesvirus 1 (BoHV-1) was examined in 110 samples of bovine nasal swabs collected between October 2007 and November 2015. BoHV-1 was detected in four samples by virus isolation and polymerase chain reaction (PCR). The phylogenetic analysis of glycoprotein B (gB) gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar to each other and branched with BoHV-1 strains isolated in Egypt and USA. BoHV-1 strains isolated in Israel, India, Brasil and USA belonging to the second branch were 100% similar to each other and 98% to 99% similar to the strains from Serbia. The phylogenetic analysis of the tk gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar and grouped together in one branch. BoHV-1 strains from USA and Australia clustered with Serbian BoHV-1 strains sharing similarity of 99%. BoHV-1 strains from India and USA clustered separately. The presented work is one of the first reports of the phylogenetic analysis of BoHV-1 isolates from Serbia.
PB  - Ecole Nationale Veterinaire Toulouse, Toulouse Cedex 3
T2  - Revue De Medecine Veterinaire
T1  - Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia
VL  - 169
IS  - 7-9
SP  - 180
EP  - 184
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1645
ER  - 

@article{
author = "Nišavić, Jakov and Knežević, Aleksandra and Stanojević, M. and Milić, Nenad and Radalj, Andrea",
year = "2018",
abstract = "The presence of bovine herpesvirus 1 (BoHV-1) was examined in 110 samples of bovine nasal swabs collected between October 2007 and November 2015. BoHV-1 was detected in four samples by virus isolation and polymerase chain reaction (PCR). The phylogenetic analysis of glycoprotein B (gB) gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar to each other and branched with BoHV-1 strains isolated in Egypt and USA. BoHV-1 strains isolated in Israel, India, Brasil and USA belonging to the second branch were 100% similar to each other and 98% to 99% similar to the strains from Serbia. The phylogenetic analysis of the tk gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar and grouped together in one branch. BoHV-1 strains from USA and Australia clustered with Serbian BoHV-1 strains sharing similarity of 99%. BoHV-1 strains from India and USA clustered separately. The presented work is one of the first reports of the phylogenetic analysis of BoHV-1 isolates from Serbia.",
publisher = "Ecole Nationale Veterinaire Toulouse, Toulouse Cedex 3",
journal = "Revue De Medecine Veterinaire",
title = "Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia",
volume = "169",
number = "7-9",
pages = "180-184",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1645"
}

Nišavić, J., Knežević, A., Stanojević, M., Milić, N.,& Radalj, A.. (2018). Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia. in Revue De Medecine Veterinaire
Ecole Nationale Veterinaire Toulouse, Toulouse Cedex 3., 169(7-9), 180-184.
https://hdl.handle.net/21.15107/rcub_veterinar_1645

Nišavić J, Knežević A, Stanojević M, Milić N, Radalj A. Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia. in Revue De Medecine Veterinaire. 2018;169(7-9):180-184.
https://hdl.handle.net/21.15107/rcub_veterinar_1645 .

Nišavić, Jakov, Knežević, Aleksandra, Stanojević, M., Milić, Nenad, Radalj, Andrea, "Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia" in Revue De Medecine Veterinaire, 169, no. 7-9 (2018):180-184,
https://hdl.handle.net/21.15107/rcub_veterinar_1645 .

TY  - CONF
AU  - Nišavić, Jakov
AU  - Milić, Nenad
AU  - Radalj, Andrea
AU  - Knežević, Aleksandra
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2269
AB  - UVOD: Goveđi herpesvirus 1 (BHV-1) pripada rodu Varicellovirus, podfamiliji Alphaherpesvirinae
i familiji Herpesviridae. Kod goveda izaziva infektivni goveđi rinotraheitis, odnosno infektivni
pustulozni vulvovaginitis. Virus izaziva značajne ekonomske štete u govedarstvu.
CILJ: Cilj ispitivanja je bila molekularna detekcija i karakterizacija sojeva BHV-1 izolovanih kod
goveda na teritoriji Republike Srbije i upoređivanje dobijenih sekvenci sa analognim sekvencama
sojeva BHV-1 iz drugih geografskih regiona.
METOD: Ispitano je 110 uzoraka nosnih briseva poreklom od nevakcinisanih goveda iz različitih
regiona u Republici Srbiji. Izolacija i identifikacija virusa BHV-1 vršena je korišćenjem ćelijske
linije Vero. Metoda PCR je izvođena uz korišćenje prajmera za delove gena koji kodiraju sintezu
glikoproteina B (gB) i timidin-kinaze (TK) BHV-1. Određivanje redosleda nukleotida dela TK i gB
gena BHV-1 je vršena metodom direktnog sekvenciranja po Sanger-u, a dobijene nukleotidne
sekvence su upoređivane sa analognim sekvencama delova prethodno navedenih gena
referentnih i izolovanih sojeva BHV-1 dostupnih u banci gena
(http://www.ncbi.nlm.nih.gov/BLAST/).
REZULTAT: Primenom metoda izolacije virusa i PCR, utvrđeno je prisustvo BHV-1 kod četiri
uzorka nosnih briseva goveda. Filogenetska analiza dela gB gena je pokazala da su nukleotidne
sekvence sojeva BHV-1 poreklom iz Srbije grupisane zajedno sa sojevima virusa poreklom iz
Egipta i SAD. Nukleotidne sekvence dela TK gena četiri izolovana soja BHV-1 poreklom iz Srbije
su međusobno imale visok stepen sličnosti i grupisane su zajedno na filogenetskom stablu,
odnosno sa sojevima virusa poreklom iz SAD i Australije.
ZAKLJUČAK: Dobijeni rezultati su pokazali visok stepen sličnosti između sojeva BHV-1 poreklom
iz Srbije i sojeva navedenog virusa poreklom iz drugih delova sveta. Pored toga, rezultati
navedenih ispitivanja su ukazali na potrebu daljih opsežnijih istraživanja koja bi imala za cilj
utvrđivanje prevalencije i molekularnih karakteristika sojeva BHV-1 kod goveda na teritoriji
Republike Srbije.
AB  - INTRODUCTION: Bovine herpesvirus 1 (BHV-1) is a member of the genus Varicellovirus of the
Alphaherpesvirinae subfamily and Herpesviridae family. It is an important bovine pathogen
causing infectious bovine rinotracheitis and infectious pustular vulvovaginitis and leads to major
economic losses in the cattle industry.
AIM: Molecular detection and characterization of BHV-1 strains isolated from cattle originating
from The Republic of Serbia and the comparison of obtained sequences with viral sequences from
other geographic regions.
METHOD: A total of 110 nasal swabs from non-vaccinated animals raised in different regions of
Serbia were examined. Vero cell line was used for virus isolation and identification. PCR was
performed using primers for the parts of the BHV-1 genome coding the synthesis of glycoprotein
B (gB) and thymidine kinase (TK). Partial sequencing of glycoprotein B (gB) and thymidine kinase
(TK) genes of BHV-1 was performed according to Sanger sequencing method and the obtained
nucleotide sequences were compared to analogous sequences of the mentioned genes available
in GenBank database (http://www.ncbi.nlm.nih.gov/BLAST/).
RESULT: BHV-1 was detected in four samples using virus isolation and PCR. The phylogenetic
analysis of partial glycoprotein B (gB) gene nucleotide sequences of all four BHV-1 strains showed
the clustering with BHV-1 strains isolated in Egypt and USA. Partial TK gene nucleotide sequences
of Serbian BHV-1 strains were highly similar and grouped together in one branch along with BHV-
1 strains from USA and Australia.
CONCLUSION: The obtained results showed a high level of similarity between BHV-1 strains from
Serbia and other parts of the world. Moreover, these results provide a prerequisite for further
detailed investigations aimed to establish the prevalence and molecular characteristics of BHV-1
strains in The Republic of Serbia.
PB  - Udruženje mikrobiologa Srbije, Beograd
C3  - XII Kongres mikrobiologa Srbije sa međunarodnim učešćem – Mikromed 2018 REGIO
T1  - Molekularna karakterizacija i filogenetska analiza sojeva goveđeg herpesvirusa 1 (BHV-1) izolovanih kod goveda na teritoriji Republike Srbije
T1  - Molecular Characterization and Phylogenetic Analysis of Bovine Herpesvirus 1 (BHV-1) Strains Isolated From Cattle in the Republic of Serbia
SP  - 151
EP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2269
ER  - 

@conference{
author = "Nišavić, Jakov and Milić, Nenad and Radalj, Andrea and Knežević, Aleksandra",
year = "2018",
abstract = "UVOD: Goveđi herpesvirus 1 (BHV-1) pripada rodu Varicellovirus, podfamiliji Alphaherpesvirinae
i familiji Herpesviridae. Kod goveda izaziva infektivni goveđi rinotraheitis, odnosno infektivni
pustulozni vulvovaginitis. Virus izaziva značajne ekonomske štete u govedarstvu.
CILJ: Cilj ispitivanja je bila molekularna detekcija i karakterizacija sojeva BHV-1 izolovanih kod
goveda na teritoriji Republike Srbije i upoređivanje dobijenih sekvenci sa analognim sekvencama
sojeva BHV-1 iz drugih geografskih regiona.
METOD: Ispitano je 110 uzoraka nosnih briseva poreklom od nevakcinisanih goveda iz različitih
regiona u Republici Srbiji. Izolacija i identifikacija virusa BHV-1 vršena je korišćenjem ćelijske
linije Vero. Metoda PCR je izvođena uz korišćenje prajmera za delove gena koji kodiraju sintezu
glikoproteina B (gB) i timidin-kinaze (TK) BHV-1. Određivanje redosleda nukleotida dela TK i gB
gena BHV-1 je vršena metodom direktnog sekvenciranja po Sanger-u, a dobijene nukleotidne
sekvence su upoređivane sa analognim sekvencama delova prethodno navedenih gena
referentnih i izolovanih sojeva BHV-1 dostupnih u banci gena
(http://www.ncbi.nlm.nih.gov/BLAST/).
REZULTAT: Primenom metoda izolacije virusa i PCR, utvrđeno je prisustvo BHV-1 kod četiri
uzorka nosnih briseva goveda. Filogenetska analiza dela gB gena je pokazala da su nukleotidne
sekvence sojeva BHV-1 poreklom iz Srbije grupisane zajedno sa sojevima virusa poreklom iz
Egipta i SAD. Nukleotidne sekvence dela TK gena četiri izolovana soja BHV-1 poreklom iz Srbije
su međusobno imale visok stepen sličnosti i grupisane su zajedno na filogenetskom stablu,
odnosno sa sojevima virusa poreklom iz SAD i Australije.
ZAKLJUČAK: Dobijeni rezultati su pokazali visok stepen sličnosti između sojeva BHV-1 poreklom
iz Srbije i sojeva navedenog virusa poreklom iz drugih delova sveta. Pored toga, rezultati
navedenih ispitivanja su ukazali na potrebu daljih opsežnijih istraživanja koja bi imala za cilj
utvrđivanje prevalencije i molekularnih karakteristika sojeva BHV-1 kod goveda na teritoriji
Republike Srbije., INTRODUCTION: Bovine herpesvirus 1 (BHV-1) is a member of the genus Varicellovirus of the
Alphaherpesvirinae subfamily and Herpesviridae family. It is an important bovine pathogen
causing infectious bovine rinotracheitis and infectious pustular vulvovaginitis and leads to major
economic losses in the cattle industry.
AIM: Molecular detection and characterization of BHV-1 strains isolated from cattle originating
from The Republic of Serbia and the comparison of obtained sequences with viral sequences from
other geographic regions.
METHOD: A total of 110 nasal swabs from non-vaccinated animals raised in different regions of
Serbia were examined. Vero cell line was used for virus isolation and identification. PCR was
performed using primers for the parts of the BHV-1 genome coding the synthesis of glycoprotein
B (gB) and thymidine kinase (TK). Partial sequencing of glycoprotein B (gB) and thymidine kinase
(TK) genes of BHV-1 was performed according to Sanger sequencing method and the obtained
nucleotide sequences were compared to analogous sequences of the mentioned genes available
in GenBank database (http://www.ncbi.nlm.nih.gov/BLAST/).
RESULT: BHV-1 was detected in four samples using virus isolation and PCR. The phylogenetic
analysis of partial glycoprotein B (gB) gene nucleotide sequences of all four BHV-1 strains showed
the clustering with BHV-1 strains isolated in Egypt and USA. Partial TK gene nucleotide sequences
of Serbian BHV-1 strains were highly similar and grouped together in one branch along with BHV-
1 strains from USA and Australia.
CONCLUSION: The obtained results showed a high level of similarity between BHV-1 strains from
Serbia and other parts of the world. Moreover, these results provide a prerequisite for further
detailed investigations aimed to establish the prevalence and molecular characteristics of BHV-1
strains in The Republic of Serbia.",
publisher = "Udruženje mikrobiologa Srbije, Beograd",
journal = "XII Kongres mikrobiologa Srbije sa međunarodnim učešćem – Mikromed 2018 REGIO",
title = "Molekularna karakterizacija i filogenetska analiza sojeva goveđeg herpesvirusa 1 (BHV-1) izolovanih kod goveda na teritoriji Republike Srbije, Molecular Characterization and Phylogenetic Analysis of Bovine Herpesvirus 1 (BHV-1) Strains Isolated From Cattle in the Republic of Serbia",
pages = "151-152",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2269"
}

Nišavić, J., Milić, N., Radalj, A.,& Knežević, A.. (2018). Molekularna karakterizacija i filogenetska analiza sojeva goveđeg herpesvirusa 1 (BHV-1) izolovanih kod goveda na teritoriji Republike Srbije. in XII Kongres mikrobiologa Srbije sa međunarodnim učešćem – Mikromed 2018 REGIO
Udruženje mikrobiologa Srbije, Beograd., 151-152.
https://hdl.handle.net/21.15107/rcub_veterinar_2269

Nišavić J, Milić N, Radalj A, Knežević A. Molekularna karakterizacija i filogenetska analiza sojeva goveđeg herpesvirusa 1 (BHV-1) izolovanih kod goveda na teritoriji Republike Srbije. in XII Kongres mikrobiologa Srbije sa međunarodnim učešćem – Mikromed 2018 REGIO. 2018;:151-152.
https://hdl.handle.net/21.15107/rcub_veterinar_2269 .

Nišavić, Jakov, Milić, Nenad, Radalj, Andrea, Knežević, Aleksandra, "Molekularna karakterizacija i filogenetska analiza sojeva goveđeg herpesvirusa 1 (BHV-1) izolovanih kod goveda na teritoriji Republike Srbije" in XII Kongres mikrobiologa Srbije sa međunarodnim učešćem – Mikromed 2018 REGIO (2018):151-152,
https://hdl.handle.net/21.15107/rcub_veterinar_2269 .

TY  - JOUR
AU  - Lukač, Bojan
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Veljović, Ljubiša
AU  - Milićević, Vesna
AU  - Zorić, Andrea
AU  - Đurić, Spomenka
AU  - Stanojević, Maja
AU  - Nišavić, Jakov
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1352
AB  - The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (15%), while porcine parvovirus DNA was identified in five lymph node samples (12.5%). The presence of both viruses was detected in three lymph node samples (7.5%). Partial nucleotide sequence of ORF1 gene of 2 porcine circovirus 2 and VP2 gene of 2 porcine parvovirus isolates was determined. The nucleotide sequences of two PCV2 isolates from RS-BIH included in phylogenetic typing are similar and cluster together with the strain Mantova isolated from domestic pigs in Italy, strains DE006-14 and DE222-13 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar to the strain NIV-C SRB isolated from pigs in Serbia. The nucleotide sequences of two PPV isolates that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China.
AB  - Prisustvo svinjskog cirkovirusa 2 i parvovirusa svinja ispitano je u četrdeset uzoraka (slezina, limfni čvorovi, pluća) poreklom od nevakcinisanih svinja primenom lančane reakcije polimeraze. Sve životinje su bile iz ekstenzivnog načina gajenja i iz različitih regiona Republike Srpske, BiH. Četiri uzorka limfnih čvorova i dva uzorka slezine su bili pozitivni na prisustvo DNK svinjskog cirkovirusa 2 (15%), dok je kod pet uzoraka limfnih čvorova utvrđeno prisustvo DNK parvovirusa svinja (12.5%). U uzorcima poreklom od tri svinje utvrđeno je prisustvo nukleinske kiseline oba prethodno navedena virusa (7.5%). Metodom sekvenciranja određena je nukleotidna sekvenca dela ORF1 gena dva izolata svinjskog cirkovirusa 2 i dela VP2 gena dva izolata parvovirusa svinja. Nukleotidne sekvence dva izolata PCV2 utvrđena u uzorcima svinja poreklom iz RS-BiH koja su bila uključena u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama soja Mantova izolovanog kod svinja u Italiji, zatim sojeva DE006-14 i DE222-13 izolovanih kod svinja u Nemačkoj kao i sa sojem Jvnan izolovanog kod svinja u Kini. Istovremeno, izolati PCV2 utvrđeni kod svinja u RS-BiH su bili delimično slični sa sojem NIV-C SRB virusa PCV2 izolovanim kod svinja u Srbiji. Nukleotidne sekvence dva izolata parvovirusa svinja uključenih u filogenetsku analizu su pokazale visok stepen sličnosti sa sojem Challenge izolovanim kod svinja u UK, sojem Kresse izolovanim kod svinja u SAD-u kao i sojevima 77 i LZ izolovanim kod svinja u Kini.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina
T1  - Molekularna detekcija PCV2 i PPV kod svinja u Republici Srpskoj, Bosna i Hercegovina
VL  - 66
IS  - 1
SP  - 51
EP  - 60
DO  - 10.1515/acve-2016-0004
ER  - 

@article{
author = "Lukač, Bojan and Knežević, Aleksandra and Milić, Nenad and Krnjaić, Dejan and Veljović, Ljubiša and Milićević, Vesna and Zorić, Andrea and Đurić, Spomenka and Stanojević, Maja and Nišavić, Jakov",
year = "2016",
abstract = "The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (15%), while porcine parvovirus DNA was identified in five lymph node samples (12.5%). The presence of both viruses was detected in three lymph node samples (7.5%). Partial nucleotide sequence of ORF1 gene of 2 porcine circovirus 2 and VP2 gene of 2 porcine parvovirus isolates was determined. The nucleotide sequences of two PCV2 isolates from RS-BIH included in phylogenetic typing are similar and cluster together with the strain Mantova isolated from domestic pigs in Italy, strains DE006-14 and DE222-13 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar to the strain NIV-C SRB isolated from pigs in Serbia. The nucleotide sequences of two PPV isolates that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China., Prisustvo svinjskog cirkovirusa 2 i parvovirusa svinja ispitano je u četrdeset uzoraka (slezina, limfni čvorovi, pluća) poreklom od nevakcinisanih svinja primenom lančane reakcije polimeraze. Sve životinje su bile iz ekstenzivnog načina gajenja i iz različitih regiona Republike Srpske, BiH. Četiri uzorka limfnih čvorova i dva uzorka slezine su bili pozitivni na prisustvo DNK svinjskog cirkovirusa 2 (15%), dok je kod pet uzoraka limfnih čvorova utvrđeno prisustvo DNK parvovirusa svinja (12.5%). U uzorcima poreklom od tri svinje utvrđeno je prisustvo nukleinske kiseline oba prethodno navedena virusa (7.5%). Metodom sekvenciranja određena je nukleotidna sekvenca dela ORF1 gena dva izolata svinjskog cirkovirusa 2 i dela VP2 gena dva izolata parvovirusa svinja. Nukleotidne sekvence dva izolata PCV2 utvrđena u uzorcima svinja poreklom iz RS-BiH koja su bila uključena u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama soja Mantova izolovanog kod svinja u Italiji, zatim sojeva DE006-14 i DE222-13 izolovanih kod svinja u Nemačkoj kao i sa sojem Jvnan izolovanog kod svinja u Kini. Istovremeno, izolati PCV2 utvrđeni kod svinja u RS-BiH su bili delimično slični sa sojem NIV-C SRB virusa PCV2 izolovanim kod svinja u Srbiji. Nukleotidne sekvence dva izolata parvovirusa svinja uključenih u filogenetsku analizu su pokazale visok stepen sličnosti sa sojem Challenge izolovanim kod svinja u UK, sojem Kresse izolovanim kod svinja u SAD-u kao i sojevima 77 i LZ izolovanim kod svinja u Kini.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina, Molekularna detekcija PCV2 i PPV kod svinja u Republici Srpskoj, Bosna i Hercegovina",
volume = "66",
number = "1",
pages = "51-60",
doi = "10.1515/acve-2016-0004"
}

Lukač, B., Knežević, A., Milić, N., Krnjaić, D., Veljović, L., Milićević, V., Zorić, A., Đurić, S., Stanojević, M.,& Nišavić, J.. (2016). Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(1), 51-60.
https://doi.org/10.1515/acve-2016-0004

Lukač B, Knežević A, Milić N, Krnjaić D, Veljović L, Milićević V, Zorić A, Đurić S, Stanojević M, Nišavić J. Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina. in Acta Veterinaria-Beograd. 2016;66(1):51-60.
doi:10.1515/acve-2016-0004 .

Lukač, Bojan, Knežević, Aleksandra, Milić, Nenad, Krnjaić, Dejan, Veljović, Ljubiša, Milićević, Vesna, Zorić, Andrea, Đurić, Spomenka, Stanojević, Maja, Nišavić, Jakov, "Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina" in Acta Veterinaria-Beograd, 66, no. 1 (2016):51-60,
https://doi.org/10.1515/acve-2016-0004 . .

TY  - JOUR
AU  - Miković, Radoš
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Radojičić, Marina
AU  - Veljović, Ljubiša
AU  - Milićević, Vesna
AU  - Zorić, Andrea
AU  - Stanojević, Maja
AU  - Nišavić, Jakov
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1359
AB  - The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identified in this study showed high similarity and significant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and significant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identified in swine in Republic of Montenegro. Besides that, these results confirmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine.
AB  - Primenom metode izolacije virusa i PCR metode, ukupno je ispitano devedeset uzoraka poreklom od svinja iz ekstenzivnog uzgoja iz različitih delova Crne Gore na prisustvo pseudorabijes virusa svinja (PrV), parvovirusa svinja (PPV) i svinjskog cirkovirusa 2 (PCV2). Primenom PCR metode prisustvo PrV je ustanovljeno kod tri uzorka, PPV kod sedam uzoraka, dok je prisustvo PCV2 utvrđeno kod šest uzoraka poreklom od svinja. Nukleotidne sekvence tri izolata PrV utvrđene u uzorcima poreklom od svinja koje su bile uključene u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama sojeva Kaplan i Bartha virusa izolovanih u Mađarskoj, zatim sa sekvencom soja Becker izolovanog u SAD-u i sekvencom soja Kolchis izolovanog u uzorcima poreklom od svinja u Grčkoj. Sva tri izolata su pripadala genotipu I pseudorabijes virusa. Nukleotidne sekvence dva izolata parvovirusa svinja su pokazale visok stepen sličnosti sa sekvencom soja Challenge izolovanog kod svinja u Velikoj Britaniji, zatim sekvencama soja Kresse izolovanog kod svinja u SAD-u i sojeva 77 i LZ izolovanih kod svinja u Kini. Filogenetska analiza nukleotidnih sekvenci dva PCV2 izolata je pokazala visok stepen sličnosti sa sojevima genotipa PCV2b kao što su NIVS-3, NIVS-5 i NIVS-6 izolovanim u Srbiji, sojem 3959 virusa izolovanim u Austriji i sojem PM165 virusa izolovanim u Brazilu i sojem XT2008 virusa izolovanim u Kini. Dobijeni rezultati ispitivanja pružili su uvid u molekularnu karakterizaciju sojeva virusa Aujeckijeve bolesti, svinjskog parvovirusa i svinjskog cirkovirusa 2 identifikovanih kod svinja u Crnoj Gori. Pored toga, dobijeni rezultati ispitivanja su potvrdili opravdanost korišćenja metode PCR u brzoj i pouzdanoj detekciji prethodno navedenih virusa kod supklinički inficiranih svinja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro
T1  - Molekularna detekcija PrV, PPV i PCV2 kod svinja u Republici Crnoj Gori
VL  - 66
IS  - 3
SP  - 347
EP  - 358
DO  - 10.1515/acve-2016-0030
ER  - 

@article{
author = "Miković, Radoš and Knežević, Aleksandra and Milić, Nenad and Krnjaić, Dejan and Radojičić, Marina and Veljović, Ljubiša and Milićević, Vesna and Zorić, Andrea and Stanojević, Maja and Nišavić, Jakov",
year = "2016",
abstract = "The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identified in this study showed high similarity and significant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and significant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identified in swine in Republic of Montenegro. Besides that, these results confirmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine., Primenom metode izolacije virusa i PCR metode, ukupno je ispitano devedeset uzoraka poreklom od svinja iz ekstenzivnog uzgoja iz različitih delova Crne Gore na prisustvo pseudorabijes virusa svinja (PrV), parvovirusa svinja (PPV) i svinjskog cirkovirusa 2 (PCV2). Primenom PCR metode prisustvo PrV je ustanovljeno kod tri uzorka, PPV kod sedam uzoraka, dok je prisustvo PCV2 utvrđeno kod šest uzoraka poreklom od svinja. Nukleotidne sekvence tri izolata PrV utvrđene u uzorcima poreklom od svinja koje su bile uključene u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama sojeva Kaplan i Bartha virusa izolovanih u Mađarskoj, zatim sa sekvencom soja Becker izolovanog u SAD-u i sekvencom soja Kolchis izolovanog u uzorcima poreklom od svinja u Grčkoj. Sva tri izolata su pripadala genotipu I pseudorabijes virusa. Nukleotidne sekvence dva izolata parvovirusa svinja su pokazale visok stepen sličnosti sa sekvencom soja Challenge izolovanog kod svinja u Velikoj Britaniji, zatim sekvencama soja Kresse izolovanog kod svinja u SAD-u i sojeva 77 i LZ izolovanih kod svinja u Kini. Filogenetska analiza nukleotidnih sekvenci dva PCV2 izolata je pokazala visok stepen sličnosti sa sojevima genotipa PCV2b kao što su NIVS-3, NIVS-5 i NIVS-6 izolovanim u Srbiji, sojem 3959 virusa izolovanim u Austriji i sojem PM165 virusa izolovanim u Brazilu i sojem XT2008 virusa izolovanim u Kini. Dobijeni rezultati ispitivanja pružili su uvid u molekularnu karakterizaciju sojeva virusa Aujeckijeve bolesti, svinjskog parvovirusa i svinjskog cirkovirusa 2 identifikovanih kod svinja u Crnoj Gori. Pored toga, dobijeni rezultati ispitivanja su potvrdili opravdanost korišćenja metode PCR u brzoj i pouzdanoj detekciji prethodno navedenih virusa kod supklinički inficiranih svinja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro, Molekularna detekcija PrV, PPV i PCV2 kod svinja u Republici Crnoj Gori",
volume = "66",
number = "3",
pages = "347-358",
doi = "10.1515/acve-2016-0030"
}

Miković, R., Knežević, A., Milić, N., Krnjaić, D., Radojičić, M., Veljović, L., Milićević, V., Zorić, A., Stanojević, M.,& Nišavić, J.. (2016). Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3), 347-358.
https://doi.org/10.1515/acve-2016-0030

Miković R, Knežević A, Milić N, Krnjaić D, Radojičić M, Veljović L, Milićević V, Zorić A, Stanojević M, Nišavić J. Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro. in Acta Veterinaria-Beograd. 2016;66(3):347-358.
doi:10.1515/acve-2016-0030 .

Miković, Radoš, Knežević, Aleksandra, Milić, Nenad, Krnjaić, Dejan, Radojičić, Marina, Veljović, Ljubiša, Milićević, Vesna, Zorić, Andrea, Stanojević, Maja, Nišavić, Jakov, "Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro" in Acta Veterinaria-Beograd, 66, no. 3 (2016):347-358,
https://doi.org/10.1515/acve-2016-0030 . .

TY  - JOUR
AU  - Veljović, Ljubiša
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Miković, Radoš
AU  - Zorić, Andrea
AU  - Marković, Maja
AU  - Milićević, Vesna
AU  - Stamenković, Miodrag
AU  - Stanojević, Maja
AU  - Maksimović-Zorić, Jelena
AU  - Petrović, Tamaš
AU  - Nišavić, Jakov
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1368
AB  - The presence of bovine parainfluenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight samples (8/119; 6.7%). The confirmation of isolated strains of BPIV3 was done by the virus-neutralization test. In addition, all samples of bovine nasal swabs were also examined for the presence of BPIV3 virus using RT-PCR with primers specific for the part of HN gene. The presence of BPIV3 was detected in eight samples (8/119; 6.7%) that were also positive upon virus isolation. The molecular characterization based on nucleotide sequencing of the part of the HN gene showed that all BPIV3 isolates belonged to genotype C of BPIV3. They branched in one distinct cluster with three different branches, but these branches were very similar to each other (98.1% to 99.8%). Serbian BPIV3c isolates were most similar to the Chinese BPIV3c isolates SD0805, SD0809 and SD0835 (from 97.92% to 99.7%), and to South Korean (12Q061), Japanese (HS9) and American (TVMDL16 and TVMDL20) BPIV3c strains (from 97.1% to 98.8%), and distinct from American (TVMDL15and TVMDL17) and Australian (Q5592) BPI3V genotype B strains (only 79.9% to 82.3% similarity), as well as from the genotype A BPIV3 strains from different countries published in GenBank.
AB  - Ukupno je ispitano 119 uzoraka nosnih briseva goveda na prisustvo parainfluenca 3 virusa goveda (bovine parainfluenza virus type, eng. - 3 BPIV3). Iz svih uzoraka nosnih briseva je vršena izolacija virusa na ćelijskoj liniji MDBK. Pojava citopatogenog efekta na kulturi ćelija, nakon 48h, odnosno 72h, utvrđena je kod osam uzoraka nosnih briseva (8/119; 6.7%). Identifikacija izolovanih sojeva BPIV3 je izvršena primenom virus neutralizacionog testa. Dodatno, svi uzorci nosnih briseva goveda su ispitani na prisustvo BPIV3 i primenom metode RT-PCR uz korišćenje prajmera specifičnih za deo HN gena virusa. Prisustvo virusne nukleinske kiseline je utvrđeno kod osam uzoraka nosnih briseva (8/119; 6.7%), koji su bili pozitivni i na izolaciji virusa. Molekularna karakterizacija zasnovana na sekvenciranju dela HN gena izolata BPIV3 iz Srbije je potvrdila da svi pripadaju genotipu C BPIV3 (BPIV3c). Oni su se u filogenetskom stablu granali u tri različite grane koje su međusobno veoma slične (98.1% do 99.8%). Izolati BPIV3 iz Srbije su pokazali visok stepen sličnosti nukleotidnih sekvenci sa BPIV3c sojevima SD0805, SD0809 i SD0835 iz Kine (97.92% do 99.7%), odnosno sa BPIV3c sojevima 12Q061 iz Južne Koreje, HS9 iz Japana i TVMDL16 i TVMDL20 iz Amerike (sličnost od 97.1% do 98.8%), kao i različitosti u odnosu na nukleotidne sekvence sojeva TVMDL15 i TVMDL17 izolovanih u Americi i soja Q5592 izolovanog u Australiji, a koji su pripadali genotipu B BPIV3 (sličnost od 79.9% do 82.3%). Slična razlika je utvrđena i sa nukleotidnim sekvencama sojeva virusa, poreklom iz različitih država, svrstanih u genotip A BPIV3, a objavljenih u genskoj bazi podataka (NCBI GenBank).
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia
T1  - Izolacija i molekularna detekcija parainfluenca 3 virusa kod goveda u Srbiji
VL  - 66
IS  - 4
SP  - 509
EP  - 519
DO  - 10.1515/acve-2016-0044
ER  - 

@article{
author = "Veljović, Ljubiša and Knežević, Aleksandra and Milić, Nenad and Krnjaić, Dejan and Miković, Radoš and Zorić, Andrea and Marković, Maja and Milićević, Vesna and Stamenković, Miodrag and Stanojević, Maja and Maksimović-Zorić, Jelena and Petrović, Tamaš and Nišavić, Jakov",
year = "2016",
abstract = "The presence of bovine parainfluenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight samples (8/119; 6.7%). The confirmation of isolated strains of BPIV3 was done by the virus-neutralization test. In addition, all samples of bovine nasal swabs were also examined for the presence of BPIV3 virus using RT-PCR with primers specific for the part of HN gene. The presence of BPIV3 was detected in eight samples (8/119; 6.7%) that were also positive upon virus isolation. The molecular characterization based on nucleotide sequencing of the part of the HN gene showed that all BPIV3 isolates belonged to genotype C of BPIV3. They branched in one distinct cluster with three different branches, but these branches were very similar to each other (98.1% to 99.8%). Serbian BPIV3c isolates were most similar to the Chinese BPIV3c isolates SD0805, SD0809 and SD0835 (from 97.92% to 99.7%), and to South Korean (12Q061), Japanese (HS9) and American (TVMDL16 and TVMDL20) BPIV3c strains (from 97.1% to 98.8%), and distinct from American (TVMDL15and TVMDL17) and Australian (Q5592) BPI3V genotype B strains (only 79.9% to 82.3% similarity), as well as from the genotype A BPIV3 strains from different countries published in GenBank., Ukupno je ispitano 119 uzoraka nosnih briseva goveda na prisustvo parainfluenca 3 virusa goveda (bovine parainfluenza virus type, eng. - 3 BPIV3). Iz svih uzoraka nosnih briseva je vršena izolacija virusa na ćelijskoj liniji MDBK. Pojava citopatogenog efekta na kulturi ćelija, nakon 48h, odnosno 72h, utvrđena je kod osam uzoraka nosnih briseva (8/119; 6.7%). Identifikacija izolovanih sojeva BPIV3 je izvršena primenom virus neutralizacionog testa. Dodatno, svi uzorci nosnih briseva goveda su ispitani na prisustvo BPIV3 i primenom metode RT-PCR uz korišćenje prajmera specifičnih za deo HN gena virusa. Prisustvo virusne nukleinske kiseline je utvrđeno kod osam uzoraka nosnih briseva (8/119; 6.7%), koji su bili pozitivni i na izolaciji virusa. Molekularna karakterizacija zasnovana na sekvenciranju dela HN gena izolata BPIV3 iz Srbije je potvrdila da svi pripadaju genotipu C BPIV3 (BPIV3c). Oni su se u filogenetskom stablu granali u tri različite grane koje su međusobno veoma slične (98.1% do 99.8%). Izolati BPIV3 iz Srbije su pokazali visok stepen sličnosti nukleotidnih sekvenci sa BPIV3c sojevima SD0805, SD0809 i SD0835 iz Kine (97.92% do 99.7%), odnosno sa BPIV3c sojevima 12Q061 iz Južne Koreje, HS9 iz Japana i TVMDL16 i TVMDL20 iz Amerike (sličnost od 97.1% do 98.8%), kao i različitosti u odnosu na nukleotidne sekvence sojeva TVMDL15 i TVMDL17 izolovanih u Americi i soja Q5592 izolovanog u Australiji, a koji su pripadali genotipu B BPIV3 (sličnost od 79.9% do 82.3%). Slična razlika je utvrđena i sa nukleotidnim sekvencama sojeva virusa, poreklom iz različitih država, svrstanih u genotip A BPIV3, a objavljenih u genskoj bazi podataka (NCBI GenBank).",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia, Izolacija i molekularna detekcija parainfluenca 3 virusa kod goveda u Srbiji",
volume = "66",
number = "4",
pages = "509-519",
doi = "10.1515/acve-2016-0044"
}

Veljović, L., Knežević, A., Milić, N., Krnjaić, D., Miković, R., Zorić, A., Marković, M., Milićević, V., Stamenković, M., Stanojević, M., Maksimović-Zorić, J., Petrović, T.,& Nišavić, J.. (2016). Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(4), 509-519.
https://doi.org/10.1515/acve-2016-0044

Veljović L, Knežević A, Milić N, Krnjaić D, Miković R, Zorić A, Marković M, Milićević V, Stamenković M, Stanojević M, Maksimović-Zorić J, Petrović T, Nišavić J. Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia. in Acta Veterinaria-Beograd. 2016;66(4):509-519.
doi:10.1515/acve-2016-0044 .

Veljović, Ljubiša, Knežević, Aleksandra, Milić, Nenad, Krnjaić, Dejan, Miković, Radoš, Zorić, Andrea, Marković, Maja, Milićević, Vesna, Stamenković, Miodrag, Stanojević, Maja, Maksimović-Zorić, Jelena, Petrović, Tamaš, Nišavić, Jakov, "Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia" in Acta Veterinaria-Beograd, 66, no. 4 (2016):509-519,
https://doi.org/10.1515/acve-2016-0044 . .

TY  - JOUR
AU  - Veljović, Ljubiša
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Nišavić, Jakov
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1092
AB  - Bovine parainfluenza 3 virus (PI3) causes respiratory infections in cattle and sheep with great economic losses in livestock. The aim of this investigation was to determine the significance of molecular methods in the identification of isolated strains of PI3 virus. Twenty cattle nasal swabs were analyzed for the presence of PI3 using the standard virology method of virus isolation in MBDK cell line and virus neutralization test. The identification of isolated strains was confirmed by RT-PCR and method of direct sequencing with primers for PI3 fusion (F) protein gene. PI3 virus was isolated and identified in four nasal swabs using the standard virology method and RT-PCR. The analysis of nucleotide sequences of isolated PI3 strains showed high similarity with sequences isolated from cattle in Asia. Our results showed that molecular methods are very useful in the diagnosis of PI3 infections as well as for the identification and characterization of PI3 strains in Serbia.
PB  - Srpsko biološko društvo, Beograd
T2  - Archives of Biological Sciences
T1  - The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle
VL  - 66
IS  - 2
SP  - 491
EP  - 496
DO  - 10.2298/ABS1402491V
ER  - 

@article{
author = "Veljović, Ljubiša and Knežević, Aleksandra and Milić, Nenad and Nišavić, Jakov",
year = "2014",
abstract = "Bovine parainfluenza 3 virus (PI3) causes respiratory infections in cattle and sheep with great economic losses in livestock. The aim of this investigation was to determine the significance of molecular methods in the identification of isolated strains of PI3 virus. Twenty cattle nasal swabs were analyzed for the presence of PI3 using the standard virology method of virus isolation in MBDK cell line and virus neutralization test. The identification of isolated strains was confirmed by RT-PCR and method of direct sequencing with primers for PI3 fusion (F) protein gene. PI3 virus was isolated and identified in four nasal swabs using the standard virology method and RT-PCR. The analysis of nucleotide sequences of isolated PI3 strains showed high similarity with sequences isolated from cattle in Asia. Our results showed that molecular methods are very useful in the diagnosis of PI3 infections as well as for the identification and characterization of PI3 strains in Serbia.",
publisher = "Srpsko biološko društvo, Beograd",
journal = "Archives of Biological Sciences",
title = "The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle",
volume = "66",
number = "2",
pages = "491-496",
doi = "10.2298/ABS1402491V"
}

Veljović, L., Knežević, A., Milić, N.,& Nišavić, J.. (2014). The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd., 66(2), 491-496.
https://doi.org/10.2298/ABS1402491V

Veljović L, Knežević A, Milić N, Nišavić J. The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle. in Archives of Biological Sciences. 2014;66(2):491-496.
doi:10.2298/ABS1402491V .

Veljović, Ljubiša, Knežević, Aleksandra, Milić, Nenad, Nišavić, Jakov, "The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle" in Archives of Biological Sciences, 66, no. 2 (2014):491-496,
https://doi.org/10.2298/ABS1402491V . .

TY  - JOUR
AU  - Nišavić, Jakov
AU  - Milić, Nenad
AU  - Knežević, Aleksandra
AU  - Jovanović, Tanja
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/662
AB  - The objective of this study was the application of polymerase chain reaction in detection of bovine herpesvirus 1 in clinical samples. Twenty samples of bovine nasal swabs were separately inoculated in Vero cells. Laboratory strain TN 41 of bovine herpesvirus 1 served as a control in the experiment. The cytopathic effects were recovered in the cell line previously inoculated with a sample of bovine nasal swab, as well as in cells inoculated with the laboratory strain TN 41 of the bovine herpesvirus 1 after a period of incubation of 24h, 36h and 48h. Identification of the isolated strains of the virus was done by the virus - neutralization test with specific immune sera against BHV 1, with a titre of 1:16. Comparative analysis of DNA fragments of the laboratory strain of BHV1 and isolated strain obtained by polymerase chain reaction with primers for viral gB glycoprotein and thymidine-kinase coding region, confirmed that the isolated strain of the virus belongs to bovine herpesvirus 1 (BHV 1).
AB  - Cilj nàših istraživanja je bio primena lančane reakcije polimeraze u dokazivanju prisustva goveđeg herpesvirusa 1 u kliničkim uzorcima materijala. Dvadeset uzoraka nosnih briseva goveda pojedinačno je inokulisano u ćelijsku liniju Vero. Referentni soj TN 41 virusa BHV 1 služio je kao pozitivna kontrola u ogledu. Posle 24h, 36h i 48h od inokulacije, utvrđena je pojava citopatogenog efekta u ćelijskoj liniji inokulisanoj uzorkom poreklom od jednog nosnog brisa goveda. Iste promene su ustanovljene i posle inokulacije referentnog soja TN 41 virusa BHV 1 u ćelijsku liniju Vero. Identifikacija izolovanog soja virusa poreklom iz uzorka nosnog brisa goveda, vršena je primenom testa virus - neutralizacije uz korišćenje specifičnog imunog seruma protiv virusa BHV 1, titra od 1:16. Uporednom analizom DNK fragmenata referentnog soja virusa BHV 1 i izolovanog soja virusa, dobijenih primenom lančane reakcije polimeraze uz korišćenje dva para prajmera koji su amplifikovali gene na molekulu DNK koji kodiraju sintezu glikoproteina B spoljašnjeg omotača virusa BHV 1 i timidin - kinaze, potvrđena je pripadnost izolovanog soja virusa goveđem herpesvirusu 1.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The application of polymerase chain reaction in detection of bovine herpesvirus 1 in clinical samples
T1  - Primena lančane reakcije polimeraze u dokazivanju prisustva goveđeg herpesvirusa 1 u kliničkim uzorcima materijala
VL  - 60
IS  - 1
SP  - 39
EP  - 48
DO  - 10.2298/AVB1001039N
ER  - 

@article{
author = "Nišavić, Jakov and Milić, Nenad and Knežević, Aleksandra and Jovanović, Tanja",
year = "2010",
abstract = "The objective of this study was the application of polymerase chain reaction in detection of bovine herpesvirus 1 in clinical samples. Twenty samples of bovine nasal swabs were separately inoculated in Vero cells. Laboratory strain TN 41 of bovine herpesvirus 1 served as a control in the experiment. The cytopathic effects were recovered in the cell line previously inoculated with a sample of bovine nasal swab, as well as in cells inoculated with the laboratory strain TN 41 of the bovine herpesvirus 1 after a period of incubation of 24h, 36h and 48h. Identification of the isolated strains of the virus was done by the virus - neutralization test with specific immune sera against BHV 1, with a titre of 1:16. Comparative analysis of DNA fragments of the laboratory strain of BHV1 and isolated strain obtained by polymerase chain reaction with primers for viral gB glycoprotein and thymidine-kinase coding region, confirmed that the isolated strain of the virus belongs to bovine herpesvirus 1 (BHV 1)., Cilj nàših istraživanja je bio primena lančane reakcije polimeraze u dokazivanju prisustva goveđeg herpesvirusa 1 u kliničkim uzorcima materijala. Dvadeset uzoraka nosnih briseva goveda pojedinačno je inokulisano u ćelijsku liniju Vero. Referentni soj TN 41 virusa BHV 1 služio je kao pozitivna kontrola u ogledu. Posle 24h, 36h i 48h od inokulacije, utvrđena je pojava citopatogenog efekta u ćelijskoj liniji inokulisanoj uzorkom poreklom od jednog nosnog brisa goveda. Iste promene su ustanovljene i posle inokulacije referentnog soja TN 41 virusa BHV 1 u ćelijsku liniju Vero. Identifikacija izolovanog soja virusa poreklom iz uzorka nosnog brisa goveda, vršena je primenom testa virus - neutralizacije uz korišćenje specifičnog imunog seruma protiv virusa BHV 1, titra od 1:16. Uporednom analizom DNK fragmenata referentnog soja virusa BHV 1 i izolovanog soja virusa, dobijenih primenom lančane reakcije polimeraze uz korišćenje dva para prajmera koji su amplifikovali gene na molekulu DNK koji kodiraju sintezu glikoproteina B spoljašnjeg omotača virusa BHV 1 i timidin - kinaze, potvrđena je pripadnost izolovanog soja virusa goveđem herpesvirusu 1.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The application of polymerase chain reaction in detection of bovine herpesvirus 1 in clinical samples, Primena lančane reakcije polimeraze u dokazivanju prisustva goveđeg herpesvirusa 1 u kliničkim uzorcima materijala",
volume = "60",
number = "1",
pages = "39-48",
doi = "10.2298/AVB1001039N"
}

Nišavić, J., Milić, N., Knežević, A.,& Jovanović, T.. (2010). The application of polymerase chain reaction in detection of bovine herpesvirus 1 in clinical samples. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(1), 39-48.
https://doi.org/10.2298/AVB1001039N

Nišavić J, Milić N, Knežević A, Jovanović T. The application of polymerase chain reaction in detection of bovine herpesvirus 1 in clinical samples. in Acta Veterinaria-Beograd. 2010;60(1):39-48.
doi:10.2298/AVB1001039N .

Nišavić, Jakov, Milić, Nenad, Knežević, Aleksandra, Jovanović, Tanja, "The application of polymerase chain reaction in detection of bovine herpesvirus 1 in clinical samples" in Acta Veterinaria-Beograd, 60, no. 1 (2010):39-48,
https://doi.org/10.2298/AVB1001039N . .

TY  - JOUR
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Ašanin, Ružica
AU  - Knežević, Aleksandra
AU  - Ašanin, Jelena
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/746
AB  - Examinations were performed on 65 samples of nasal smeas taken from calves and young cows with clinical symptoms of respiratory infection to determine the presence of the bovine herpes virus 1 using parallel implementation of molecular and standard methods of virological diagnostics. The appearance of a cytopathogenic effect (CPE) was not established in inoculated cell lines 24h, 48h and 72h following inoculation, or after two successive passages of the examined material sample through these cell lines. The application of polymerize chain reaction (PCR) using a primer for glucoprotein B and thymidine - kinasis, established the presence of bovine herpes virus 1 nucleic acid in one sample of a bovine nasal smear, while the presence of this virus was established in three samples in an examination of the nasal smear samples using the Real-Time PCR method.
AB  - Ukupno je ispitivano 65 uzoraka nosnih briseva prikupljenih od teladi i junadi sa kliničkim simptomima respiratorne infekcije na prisustvo goveđeg herpesvirusa 1 uporednom primenom molekularnih i standardnih metoda virusološke dijagnostike. Kod inokulisanih ćelijskih linija nije ustanovljena pojava citopatogenog efekta (CPE -) posle 24h, 48h i 72h od inokulacije ni posle dve uzastopne pasaže uzoraka ispitivanog materijala kroz navedene ćelijske linije. Primenom lančane reakcije polimeraze (PCR) uz korišćenje prajmera za glikoprotein B i timidin-kinazu, utvrđeno je prisustvo nukleinske kiseline goveđeg herpesvirusa 1 u jednom uzorku nosnog brisa, dok je ispitivanjem navedenih uzoraka nosnih briseva goveda metodom Real-Time PCR prisustvo pomenutog virusa ustanovljeno kod tri uzorka.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1
T1  - Primena lančane reakcije polimeraze (PCR) i metode Real-Time PCR u brzoj identifikaciji goveđeg herpesvirusa 1
VL  - 64
IS  - 3-4
SP  - 159
EP  - 167
DO  - 10.2298/VETGL1004159M
ER  - 

@article{
author = "Milić, Nenad and Nišavić, Jakov and Ašanin, Ružica and Knežević, Aleksandra and Ašanin, Jelena and Vidanović, Dejan and Šekler, Milanko",
year = "2010",
abstract = "Examinations were performed on 65 samples of nasal smeas taken from calves and young cows with clinical symptoms of respiratory infection to determine the presence of the bovine herpes virus 1 using parallel implementation of molecular and standard methods of virological diagnostics. The appearance of a cytopathogenic effect (CPE) was not established in inoculated cell lines 24h, 48h and 72h following inoculation, or after two successive passages of the examined material sample through these cell lines. The application of polymerize chain reaction (PCR) using a primer for glucoprotein B and thymidine - kinasis, established the presence of bovine herpes virus 1 nucleic acid in one sample of a bovine nasal smear, while the presence of this virus was established in three samples in an examination of the nasal smear samples using the Real-Time PCR method., Ukupno je ispitivano 65 uzoraka nosnih briseva prikupljenih od teladi i junadi sa kliničkim simptomima respiratorne infekcije na prisustvo goveđeg herpesvirusa 1 uporednom primenom molekularnih i standardnih metoda virusološke dijagnostike. Kod inokulisanih ćelijskih linija nije ustanovljena pojava citopatogenog efekta (CPE -) posle 24h, 48h i 72h od inokulacije ni posle dve uzastopne pasaže uzoraka ispitivanog materijala kroz navedene ćelijske linije. Primenom lančane reakcije polimeraze (PCR) uz korišćenje prajmera za glikoprotein B i timidin-kinazu, utvrđeno je prisustvo nukleinske kiseline goveđeg herpesvirusa 1 u jednom uzorku nosnog brisa, dok je ispitivanjem navedenih uzoraka nosnih briseva goveda metodom Real-Time PCR prisustvo pomenutog virusa ustanovljeno kod tri uzorka.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1, Primena lančane reakcije polimeraze (PCR) i metode Real-Time PCR u brzoj identifikaciji goveđeg herpesvirusa 1",
volume = "64",
number = "3-4",
pages = "159-167",
doi = "10.2298/VETGL1004159M"
}

Milić, N., Nišavić, J., Ašanin, R., Knežević, A., Ašanin, J., Vidanović, D.,& Šekler, M.. (2010). Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(3-4), 159-167.
https://doi.org/10.2298/VETGL1004159M

Milić N, Nišavić J, Ašanin R, Knežević A, Ašanin J, Vidanović D, Šekler M. Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1. in Veterinarski Glasnik. 2010;64(3-4):159-167.
doi:10.2298/VETGL1004159M .

Milić, Nenad, Nišavić, Jakov, Ašanin, Ružica, Knežević, Aleksandra, Ašanin, Jelena, Vidanović, Dejan, Šekler, Milanko, "Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1" in Veterinarski Glasnik, 64, no. 3-4 (2010):159-167,
https://doi.org/10.2298/VETGL1004159M . .