TY  - CONF
AU  - Radulović, Stamen
AU  - Šefer, Dragan
AU  - Marković, Radmila
AU  - Jokić, Živan
AU  - Rašić, Zoran
AU  - Lovrić, Saša
AU  - Kojičić Stefanović, Jasmina
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2993
AB  - Upotreba enzima fitaze u ishrani životinja ima važan nutritivni, ekološki i ekonomski značaj. Danas postoje dva osnovna načina njene primene u praksi: korišćenjem
matrica ili dodavanjem “on the top”. S obzirom na prednosti koje pruža krajnjem korisniku, upotreba matrica postaje imperativ u savremenoj stočarskoj proizvodnji. Dobro
je poznato da u slučajevima, kada cene nabavnih sirovina (kukuruza, pšenice, masti i
neorganskog fosfora – MCP) rastu na tržištu, tada upotreba enzima u hrani postaje
ekonomski atraktivnija i pruža korisniku znatno veću dobit u odnosu na početna ulaganja. Bez obzira što proizvođači fitaze preporučuju njenu upotrebu u znatno većim količinama u odnosu na raniju praksu, neophodno je napomenuti da su u pojedinim istraživanjima zapaženi i negativni efekti takve upotrebe (pad proizvodnih rezultata i poremećaji zdravstvenog stanja životinja. Novi tehnološki postupci u proizvodnji fitaze imaju za cilj da navedene probleme prevaziđu, a kroz tzv. „superdoziranje” fitaze upotrebu
neorganskog fosfora dodatno smanje, ili ga u potpunosti isključe iz recepture. Ipak, pri
svakoj odluci o primeni fitaze neophodno je uzeti u obzir cenu preparata, njegovu optimalnu količinu i uštedu koja se može ostvariti primenom matrice (kroz smanjenje cene
recepture, poboljšanje proizvodnih rezulta i zdravstvenog stanja tretiranih životinja).
AB  - The use of phytase in animal nutrition has important nutritional, ecological and
economic importance. There are two basic ways of its application in practice: through
the use of matrices or on top. Considering the unequivocal advantages it provides to
the end user, the use of matrices becomes imperative in modern livestock production.
It is well known that in all cases when the prices of corn, wheat, fat, and in the case of
phytase, especially inorganic phosphorus (MCP), increase in the market, then the use
of enzymes in feed becomes more economically attractive and provides the user with a
significantly higher return for initial investment. Although the producers of phytase recommend its use in much larger quantities compared to the previous practice, it is necessary to mention that in some studies the negative effects of such use have also been
noted (decrease in production results, and in some cases also disturbances in the health
of animals). New technological procedures in the production of phytase aim to overcome the aforementioned effects, and through the so-called superdosing of phytase further reduce the use of inorganic phosphorus or even completely excludes it from the feed
mixtures. However, with every decision on the application of phytase, it is necessary to
take into account the price of the preparation, its optimal amount and the savings that
can be achieved by applying the matrix, as well as the effects on the production results
and/or the health status of the treated animals.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 33. Savetovanje Veterinara Srbije, Zlatibor, 08 - 11. Septembar 2022
T1  - Upotreba fitaze pri formulaciji obroka za ishranu monogastričnih životinja u cilju smanjenog ozlučivanja fosfora u spoljašnju sredinu: praktičan pristup
T1  - The use of phytase in the formulation of rations for the feeding of monogastric animals in order to reduce the excretion of phosphorus into the environment: a practical approach
SP  - 199
EP  - 210
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2993
ER  - 

@conference{
author = "Radulović, Stamen and Šefer, Dragan and Marković, Radmila and Jokić, Živan and Rašić, Zoran and Lovrić, Saša and Kojičić Stefanović, Jasmina",
year = "2022",
abstract = "Upotreba enzima fitaze u ishrani životinja ima važan nutritivni, ekološki i ekonomski značaj. Danas postoje dva osnovna načina njene primene u praksi: korišćenjem
matrica ili dodavanjem “on the top”. S obzirom na prednosti koje pruža krajnjem korisniku, upotreba matrica postaje imperativ u savremenoj stočarskoj proizvodnji. Dobro
je poznato da u slučajevima, kada cene nabavnih sirovina (kukuruza, pšenice, masti i
neorganskog fosfora – MCP) rastu na tržištu, tada upotreba enzima u hrani postaje
ekonomski atraktivnija i pruža korisniku znatno veću dobit u odnosu na početna ulaganja. Bez obzira što proizvođači fitaze preporučuju njenu upotrebu u znatno većim količinama u odnosu na raniju praksu, neophodno je napomenuti da su u pojedinim istraživanjima zapaženi i negativni efekti takve upotrebe (pad proizvodnih rezultata i poremećaji zdravstvenog stanja životinja. Novi tehnološki postupci u proizvodnji fitaze imaju za cilj da navedene probleme prevaziđu, a kroz tzv. „superdoziranje” fitaze upotrebu
neorganskog fosfora dodatno smanje, ili ga u potpunosti isključe iz recepture. Ipak, pri
svakoj odluci o primeni fitaze neophodno je uzeti u obzir cenu preparata, njegovu optimalnu količinu i uštedu koja se može ostvariti primenom matrice (kroz smanjenje cene
recepture, poboljšanje proizvodnih rezulta i zdravstvenog stanja tretiranih životinja)., The use of phytase in animal nutrition has important nutritional, ecological and
economic importance. There are two basic ways of its application in practice: through
the use of matrices or on top. Considering the unequivocal advantages it provides to
the end user, the use of matrices becomes imperative in modern livestock production.
It is well known that in all cases when the prices of corn, wheat, fat, and in the case of
phytase, especially inorganic phosphorus (MCP), increase in the market, then the use
of enzymes in feed becomes more economically attractive and provides the user with a
significantly higher return for initial investment. Although the producers of phytase recommend its use in much larger quantities compared to the previous practice, it is necessary to mention that in some studies the negative effects of such use have also been
noted (decrease in production results, and in some cases also disturbances in the health
of animals). New technological procedures in the production of phytase aim to overcome the aforementioned effects, and through the so-called superdosing of phytase further reduce the use of inorganic phosphorus or even completely excludes it from the feed
mixtures. However, with every decision on the application of phytase, it is necessary to
take into account the price of the preparation, its optimal amount and the savings that
can be achieved by applying the matrix, as well as the effects on the production results
and/or the health status of the treated animals.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "33. Savetovanje Veterinara Srbije, Zlatibor, 08 - 11. Septembar 2022",
title = "Upotreba fitaze pri formulaciji obroka za ishranu monogastričnih životinja u cilju smanjenog ozlučivanja fosfora u spoljašnju sredinu: praktičan pristup, The use of phytase in the formulation of rations for the feeding of monogastric animals in order to reduce the excretion of phosphorus into the environment: a practical approach",
pages = "199-210",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2993"
}

Radulović, S., Šefer, D., Marković, R., Jokić, Ž., Rašić, Z., Lovrić, S.,& Kojičić Stefanović, J.. (2022). Upotreba fitaze pri formulaciji obroka za ishranu monogastričnih životinja u cilju smanjenog ozlučivanja fosfora u spoljašnju sredinu: praktičan pristup. in 33. Savetovanje Veterinara Srbije, Zlatibor, 08 - 11. Septembar 2022
Beograd : Srpsko veterinarsko društvo., 199-210.
https://hdl.handle.net/21.15107/rcub_veterinar_2993

Radulović S, Šefer D, Marković R, Jokić Ž, Rašić Z, Lovrić S, Kojičić Stefanović J. Upotreba fitaze pri formulaciji obroka za ishranu monogastričnih životinja u cilju smanjenog ozlučivanja fosfora u spoljašnju sredinu: praktičan pristup. in 33. Savetovanje Veterinara Srbije, Zlatibor, 08 - 11. Septembar 2022. 2022;:199-210.
https://hdl.handle.net/21.15107/rcub_veterinar_2993 .

Radulović, Stamen, Šefer, Dragan, Marković, Radmila, Jokić, Živan, Rašić, Zoran, Lovrić, Saša, Kojičić Stefanović, Jasmina, "Upotreba fitaze pri formulaciji obroka za ishranu monogastričnih životinja u cilju smanjenog ozlučivanja fosfora u spoljašnju sredinu: praktičan pristup" in 33. Savetovanje Veterinara Srbije, Zlatibor, 08 - 11. Septembar 2022 (2022):199-210,
https://hdl.handle.net/21.15107/rcub_veterinar_2993 .

TY  - JOUR
AU  - Dacić, M.
AU  - Resanović, Radmila
AU  - Rašić, Zoran
AU  - Valčić, Miroslav
AU  - Milovanović, A.
AU  - Velhner, Maja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1623
AB  - The infectious bursal disease virus (IBDv) is widespread in poultry flocks all around the world. Various biotypes have emerged and because of that, adequate management practices and vaccination of chicks are of paramount importance for the protection against field strains. One day old Lohmann Brown chicks were vaccinated with intermediate vaccines and the recombinant VAXXITEK HVT-IBDv vaccine formulation, and challenged at 48 days of life with the very virulent IBDv (vvIBDv) strain CH/99. The best protection (100%) was achieved with the recombinant vaccine administered by the subcutaneous or intramuscular route at a day old, while intermediate and intermediate plus vaccines protected 80% of birds from clinical symptoms. The highest bursa body ratio (5.33, 3.50 and 4.12) was accomplished in non-vaccinated and non-challenged birds and birds vaccinated with the VAXXITEK HVT-IBDv vaccine. The recombinant VAXXITEK HVT-IBDv vaccine has provided protection for commercial chicks against challenge with the vvIBDv strain in this experiment. Under field conditions, additional vaccination is possibly needed with supplementary application of live attenuated vaccines. However, the recombinant vector vaccines are providing significant aid against clinical signs and immunosupression caused by the vvIBDv.
PB  - Hellenic Veterinary Medical Soc, Athens
T2  - Journal of The Hellenic Veterinary Medical Society
T1  - Efficacy of recombinant VAXXITEK HVT-IBDv vaccine against very virulent Infectious bursal disease virus (vvIBDv) challenge in layer chicks: A pilot study
VL  - 69
IS  - 1
SP  - 823
EP  - 830
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1623
ER  - 

@article{
author = "Dacić, M. and Resanović, Radmila and Rašić, Zoran and Valčić, Miroslav and Milovanović, A. and Velhner, Maja",
year = "2018",
abstract = "The infectious bursal disease virus (IBDv) is widespread in poultry flocks all around the world. Various biotypes have emerged and because of that, adequate management practices and vaccination of chicks are of paramount importance for the protection against field strains. One day old Lohmann Brown chicks were vaccinated with intermediate vaccines and the recombinant VAXXITEK HVT-IBDv vaccine formulation, and challenged at 48 days of life with the very virulent IBDv (vvIBDv) strain CH/99. The best protection (100%) was achieved with the recombinant vaccine administered by the subcutaneous or intramuscular route at a day old, while intermediate and intermediate plus vaccines protected 80% of birds from clinical symptoms. The highest bursa body ratio (5.33, 3.50 and 4.12) was accomplished in non-vaccinated and non-challenged birds and birds vaccinated with the VAXXITEK HVT-IBDv vaccine. The recombinant VAXXITEK HVT-IBDv vaccine has provided protection for commercial chicks against challenge with the vvIBDv strain in this experiment. Under field conditions, additional vaccination is possibly needed with supplementary application of live attenuated vaccines. However, the recombinant vector vaccines are providing significant aid against clinical signs and immunosupression caused by the vvIBDv.",
publisher = "Hellenic Veterinary Medical Soc, Athens",
journal = "Journal of The Hellenic Veterinary Medical Society",
title = "Efficacy of recombinant VAXXITEK HVT-IBDv vaccine against very virulent Infectious bursal disease virus (vvIBDv) challenge in layer chicks: A pilot study",
volume = "69",
number = "1",
pages = "823-830",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1623"
}

Dacić, M., Resanović, R., Rašić, Z., Valčić, M., Milovanović, A.,& Velhner, M.. (2018). Efficacy of recombinant VAXXITEK HVT-IBDv vaccine against very virulent Infectious bursal disease virus (vvIBDv) challenge in layer chicks: A pilot study. in Journal of The Hellenic Veterinary Medical Society
Hellenic Veterinary Medical Soc, Athens., 69(1), 823-830.
https://hdl.handle.net/21.15107/rcub_veterinar_1623

Dacić M, Resanović R, Rašić Z, Valčić M, Milovanović A, Velhner M. Efficacy of recombinant VAXXITEK HVT-IBDv vaccine against very virulent Infectious bursal disease virus (vvIBDv) challenge in layer chicks: A pilot study. in Journal of The Hellenic Veterinary Medical Society. 2018;69(1):823-830.
https://hdl.handle.net/21.15107/rcub_veterinar_1623 .

Dacić, M., Resanović, Radmila, Rašić, Zoran, Valčić, Miroslav, Milovanović, A., Velhner, Maja, "Efficacy of recombinant VAXXITEK HVT-IBDv vaccine against very virulent Infectious bursal disease virus (vvIBDv) challenge in layer chicks: A pilot study" in Journal of The Hellenic Veterinary Medical Society, 69, no. 1 (2018):823-830,
https://hdl.handle.net/21.15107/rcub_veterinar_1623 .

TY  - JOUR
AU  - Pajić, Marija
AU  - Boboš, Stanko
AU  - Velebit, Branko
AU  - Rašić, Zoran
AU  - Katić, Vera
AU  - Radinović, Miodrag
AU  - Nikolić, Aleksandra
AU  - Simonović, Dušan
AU  - Babić, Milijana
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1355
AB  - Staphylococcus aureus is known worldwide as a frequent cause of mastitis in dairy cattle. Due to the production of heath resistant enterotoxins, this pathogen is also a major cause of food poisoning among humans, with symptoms of often severe vomiting and diarrhea. The aim of our study was to determine the prevalence of enterotoxinproducing strains of S. aureus originating from samples of cows with subclinical and clinical mastitis in the Republic of Serbia. Furthermore, we analyzed the type of staphylococcal enterotoxin they produce and phylogenetic relatedness among the S. aureus isolates recovered from milk in this study. Production of staphylococcal enterotoxins A, B, C, D and E was determined by commercial immunoenzyme assay VIDAS® SET2, and presence of corresponding genes encoding enterotoxin synthesis in positive isolates confirmed by Polymerase Chain Reaction. Enterotoxin production was determined in 5 out of 75 (6.67%) isolates of S. aureus and all of them produced staphylococcal enterotoxins C. After analyzing the nucleotide sequence of the gene encoding the synthesis of staphylococcal protein A, S. aureus isolates were assigned into 2 phylogenetic groups, including 7 clusters. All S. aureus isolates with the presence of sec gene formed one cluster even dough they originated from milk samples from different farms.
AB  - Širom sveta S. aureus poznat je kao čest uzročnik mastitisa krava. Takođe predstavlja i glavni uzrok trovanja hranom nakon konzumiranja hrane kontaminirane njegovim enterotoksinima. Cilj ovog istraživanja bio je da se ispita prevalencija enterotoksinprodukujućih sojeva S. aureus poreklom iz vimena krava sa subkliničkim i kliničkim mastitisom u Srbiji, da se odredi tip enterotoksina koji produkuju i da se odredi filogenetska srodnost ovih izolata. Za određivanje sposobnosti sinteze stafilokoknih enterotoksina A, B, C, D i E korišćen je VIDAS® SET2 immunoenzimski test i utvrđeno je da 5 od 75 (6,67%) izolata S. aureus sintetiše enterotoksine. Lančanom reakcijom polimeraze na prisustvo odgovarajućih gena, utvrđeno je da svih 5 izolata poseduju gen za sintezu enterotoksina C. Analizom nukleotidnih sekvenci gena za sintezu stafilokoknog proteina A, izolati S. aureus grupisani su filogenetski u 2 grupe, odnosno u 7 klastera. Svi izolati S. aureus kod kojih je dokazano prisustvo gena za sintezu enterotoksina spadaju u isti klaster.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows
T1  - Prevalencija i molekularna karakterizacija enterotoksin-produkujućih sojeva S. aureus izolovanih iz vimena krava u Srbiji
VL  - 66
IS  - 4
SP  - 466
EP  - 477
DO  - 10.1515/acve-2016-0040
ER  - 

@article{
author = "Pajić, Marija and Boboš, Stanko and Velebit, Branko and Rašić, Zoran and Katić, Vera and Radinović, Miodrag and Nikolić, Aleksandra and Simonović, Dušan and Babić, Milijana",
year = "2016",
abstract = "Staphylococcus aureus is known worldwide as a frequent cause of mastitis in dairy cattle. Due to the production of heath resistant enterotoxins, this pathogen is also a major cause of food poisoning among humans, with symptoms of often severe vomiting and diarrhea. The aim of our study was to determine the prevalence of enterotoxinproducing strains of S. aureus originating from samples of cows with subclinical and clinical mastitis in the Republic of Serbia. Furthermore, we analyzed the type of staphylococcal enterotoxin they produce and phylogenetic relatedness among the S. aureus isolates recovered from milk in this study. Production of staphylococcal enterotoxins A, B, C, D and E was determined by commercial immunoenzyme assay VIDAS® SET2, and presence of corresponding genes encoding enterotoxin synthesis in positive isolates confirmed by Polymerase Chain Reaction. Enterotoxin production was determined in 5 out of 75 (6.67%) isolates of S. aureus and all of them produced staphylococcal enterotoxins C. After analyzing the nucleotide sequence of the gene encoding the synthesis of staphylococcal protein A, S. aureus isolates were assigned into 2 phylogenetic groups, including 7 clusters. All S. aureus isolates with the presence of sec gene formed one cluster even dough they originated from milk samples from different farms., Širom sveta S. aureus poznat je kao čest uzročnik mastitisa krava. Takođe predstavlja i glavni uzrok trovanja hranom nakon konzumiranja hrane kontaminirane njegovim enterotoksinima. Cilj ovog istraživanja bio je da se ispita prevalencija enterotoksinprodukujućih sojeva S. aureus poreklom iz vimena krava sa subkliničkim i kliničkim mastitisom u Srbiji, da se odredi tip enterotoksina koji produkuju i da se odredi filogenetska srodnost ovih izolata. Za određivanje sposobnosti sinteze stafilokoknih enterotoksina A, B, C, D i E korišćen je VIDAS® SET2 immunoenzimski test i utvrđeno je da 5 od 75 (6,67%) izolata S. aureus sintetiše enterotoksine. Lančanom reakcijom polimeraze na prisustvo odgovarajućih gena, utvrđeno je da svih 5 izolata poseduju gen za sintezu enterotoksina C. Analizom nukleotidnih sekvenci gena za sintezu stafilokoknog proteina A, izolati S. aureus grupisani su filogenetski u 2 grupe, odnosno u 7 klastera. Svi izolati S. aureus kod kojih je dokazano prisustvo gena za sintezu enterotoksina spadaju u isti klaster.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows, Prevalencija i molekularna karakterizacija enterotoksin-produkujućih sojeva S. aureus izolovanih iz vimena krava u Srbiji",
volume = "66",
number = "4",
pages = "466-477",
doi = "10.1515/acve-2016-0040"
}

Pajić, M., Boboš, S., Velebit, B., Rašić, Z., Katić, V., Radinović, M., Nikolić, A., Simonović, D.,& Babić, M.. (2016). Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(4), 466-477.
https://doi.org/10.1515/acve-2016-0040

Pajić M, Boboš S, Velebit B, Rašić Z, Katić V, Radinović M, Nikolić A, Simonović D, Babić M. Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows. in Acta Veterinaria-Beograd. 2016;66(4):466-477.
doi:10.1515/acve-2016-0040 .

Pajić, Marija, Boboš, Stanko, Velebit, Branko, Rašić, Zoran, Katić, Vera, Radinović, Miodrag, Nikolić, Aleksandra, Simonović, Dušan, Babić, Milijana, "Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows" in Acta Veterinaria-Beograd, 66, no. 4 (2016):466-477,
https://doi.org/10.1515/acve-2016-0040 . .

TY  - JOUR
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Borozan, Sunčica
AU  - Zorić, Andrea
AU  - Lazić, Sava
AU  - Petrović, Tamaš
AU  - Rašić, Zoran
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1221
AB  - The objective of our work was to investigate some biological characteristics of purified glycoprotein subunits of Newcastle disease virus strain PHY-LMV.42 isolated from pigeons for the purpose of vaccine production. PHY-LMV.42 strain of Newcastle disease virus was multiplied by successive passages in embryonated eggs and identified by the methods of Reverse transcriptase PCR and Real-Time PCR along with F gene sequencing. Proving the presence of HN and F antigene in the virus subunits samples was carried out by hemagglutination inhibition method with referent immune sera. Biochemical characterization of glycoprotein subunits was performed by SDS-PAGE method as well as liquid chromatography with mass spectrometry (LC ESI-TOF-MS/MS). Testing for the virus subunits immunogenicity was carried out in biological experiment on 75 laying hens Tetra-SSL and 25 chickens Isa Brown by inducing an artificial infection with Hertz 33 strain of the virus. Low concentrations of the virus antigens of 0.36 mg/ml along with glycoprotein fractions of 77 i 58 kDa manifested a strong hemagglutination activity of 4096 HJ/0,1ml. The subunit vaccines of 256 and 128 HJ/0.5 ml induced a protective immune response in all the vaccinated animals. Based on the obtained results it can be concluded that low concentrations of purified virus subunits of PHY-LMV.42 strain can be used for preparing of effective vaccines.
AB  - Cilj našeg istraživanja je bilo ispitivanje bioloških karakteristika prečišćenih glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti izolovanog iz golubova radi njihovog korišćenja za pripremanje vakcine. Soj PHY-LMV.42 virusa Newcastle bolesti je umnožavan sukcesivnim pasažama u kokošijim embrionima i identifikovan metodama Reverse transcriptase PCR i Real- Time PCR uz sekvenciranje F gena. Dokazivanje prisustva HN i F antigena u uzorcima virusnih subjedinica vršeno je metodom inhibicije hemaglutinacije sa referentnim imunim serumima. Biohemijska karakterizacija glikoproteinskih subjedinica izvršena je primenom metoda SDS-PAGE i tečne hromatografije sa masenom spektrometrijom (LC ESI-TOF-MS/MS). Ispitivanje imunogenosti virusnih subjedinica sprovedeno je u biološkom ogledu na ukupno 75 kokoši nosilja Tetra-SSL i 25 pilića Isa Brown uz izvođenje veštačke infekcije sojem Hertz 33 navedenog virusa. Niske koncentracije virusnih antigena od 0,36 mg/ml sa glikoproteinskim frakcijama od 77 i 58 kDa su ispoljavale snažnu hemaglutinacionu aktivnost od 4096 HJ/0,1ml. Subjedinične vakcine od 256 i 128 HJ/0,5 ml, indukovale su imunološki odgovor zaštitnog karaktera kod svih vakcinisanih životinja. Na osnovu dobijenih rezultata može se zaključiti da se niske koncentracije prečišćenih virusnih subjedinica soja PHY-LMV.42 mogu koristiti za pripremanje efikasne vakcine. PR Projekat Ministarstva nauke Republike Srbije, br. TR 31008.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus
T1  - Ispitivanje nekih bioloških karakteristika glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti živine
VL  - 69
IS  - 5-6
SP  - 337
EP  - 355
DO  - 10.2298/VETGL1506337M
ER  - 

@article{
author = "Milić, Nenad and Nišavić, Jakov and Borozan, Sunčica and Zorić, Andrea and Lazić, Sava and Petrović, Tamaš and Rašić, Zoran",
year = "2015",
abstract = "The objective of our work was to investigate some biological characteristics of purified glycoprotein subunits of Newcastle disease virus strain PHY-LMV.42 isolated from pigeons for the purpose of vaccine production. PHY-LMV.42 strain of Newcastle disease virus was multiplied by successive passages in embryonated eggs and identified by the methods of Reverse transcriptase PCR and Real-Time PCR along with F gene sequencing. Proving the presence of HN and F antigene in the virus subunits samples was carried out by hemagglutination inhibition method with referent immune sera. Biochemical characterization of glycoprotein subunits was performed by SDS-PAGE method as well as liquid chromatography with mass spectrometry (LC ESI-TOF-MS/MS). Testing for the virus subunits immunogenicity was carried out in biological experiment on 75 laying hens Tetra-SSL and 25 chickens Isa Brown by inducing an artificial infection with Hertz 33 strain of the virus. Low concentrations of the virus antigens of 0.36 mg/ml along with glycoprotein fractions of 77 i 58 kDa manifested a strong hemagglutination activity of 4096 HJ/0,1ml. The subunit vaccines of 256 and 128 HJ/0.5 ml induced a protective immune response in all the vaccinated animals. Based on the obtained results it can be concluded that low concentrations of purified virus subunits of PHY-LMV.42 strain can be used for preparing of effective vaccines., Cilj našeg istraživanja je bilo ispitivanje bioloških karakteristika prečišćenih glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti izolovanog iz golubova radi njihovog korišćenja za pripremanje vakcine. Soj PHY-LMV.42 virusa Newcastle bolesti je umnožavan sukcesivnim pasažama u kokošijim embrionima i identifikovan metodama Reverse transcriptase PCR i Real- Time PCR uz sekvenciranje F gena. Dokazivanje prisustva HN i F antigena u uzorcima virusnih subjedinica vršeno je metodom inhibicije hemaglutinacije sa referentnim imunim serumima. Biohemijska karakterizacija glikoproteinskih subjedinica izvršena je primenom metoda SDS-PAGE i tečne hromatografije sa masenom spektrometrijom (LC ESI-TOF-MS/MS). Ispitivanje imunogenosti virusnih subjedinica sprovedeno je u biološkom ogledu na ukupno 75 kokoši nosilja Tetra-SSL i 25 pilića Isa Brown uz izvođenje veštačke infekcije sojem Hertz 33 navedenog virusa. Niske koncentracije virusnih antigena od 0,36 mg/ml sa glikoproteinskim frakcijama od 77 i 58 kDa su ispoljavale snažnu hemaglutinacionu aktivnost od 4096 HJ/0,1ml. Subjedinične vakcine od 256 i 128 HJ/0,5 ml, indukovale su imunološki odgovor zaštitnog karaktera kod svih vakcinisanih životinja. Na osnovu dobijenih rezultata može se zaključiti da se niske koncentracije prečišćenih virusnih subjedinica soja PHY-LMV.42 mogu koristiti za pripremanje efikasne vakcine. PR Projekat Ministarstva nauke Republike Srbije, br. TR 31008.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus, Ispitivanje nekih bioloških karakteristika glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti živine",
volume = "69",
number = "5-6",
pages = "337-355",
doi = "10.2298/VETGL1506337M"
}

Milić, N., Nišavić, J., Borozan, S., Zorić, A., Lazić, S., Petrović, T.,& Rašić, Z.. (2015). Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(5-6), 337-355.
https://doi.org/10.2298/VETGL1506337M

Milić N, Nišavić J, Borozan S, Zorić A, Lazić S, Petrović T, Rašić Z. Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus. in Veterinarski Glasnik. 2015;69(5-6):337-355.
doi:10.2298/VETGL1506337M .

Milić, Nenad, Nišavić, Jakov, Borozan, Sunčica, Zorić, Andrea, Lazić, Sava, Petrović, Tamaš, Rašić, Zoran, "Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus" in Veterinarski Glasnik, 69, no. 5-6 (2015):337-355,
https://doi.org/10.2298/VETGL1506337M . .

TY  - JOUR
AU  - Resanović, Radmila
AU  - Rašić, Zoran
AU  - Kureljušić, Branislav
AU  - Vučićević, Ivana
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/657
AB  - With the increased demand of consumers for safe poultry products, efficient control of pathogenic microorganisms that cause diseases in humans is becoming the chief challenge in contemporary poultry production. Poultry meat, eggs and related products present the main source of salmonellosis in humans that occurs as a result of the consumption of contaminated food. Any serotype of Salmonella spp. that is not strictly linked to the host is capable of causing gastrointestinal disorders of various degrees in humans. However, according to the World Health Organization (WHO), Salmonella enterica serotype enteritidis has been the leading cause of salmonellosis in humans over the past 10 years. One of the available prophylactic measures is resorting to the vaccination of poultry against salmonellosis. Several vaccines against poultry salmonellosis, produced by different manufacturers, have been registered in Serbia. In order for these vaccines to become widely used in our country, it is necessary that the factors in primary poultry production are aquainted with the mechanisms of action of these vaccines, the effects achieved by their implementation, the economic feasibility, and the possibilities for monitoring in keeping with the valid legal regulations. Certainly, vaccination should not be taken as the only method in the prophylaxis of salmonellosis, but as one of the very important methods in a series of biosafety and other measures that should be applied on poultry farms for the successful curbing of salmonellosis infections.
AB  - Sa povećanim zahtevima potrošača za bezbednim živinskim proizvodima, efikasna kontrola patogenih mikroorganizama koji izazivaju oboljenja kod ljudi, postaje glavni izazov u savremenoj živinarskoj proizvodnji. Živinsko meso, jaja i proizvodi od njih predstavjaju glavni izvor salmoneloze kod ljudi koja nastaje usled konzumiranja kontaminirane hrane. Bilo koji serotip Salmonella spp. koji nije striktno vezan za domaćina sposoban je da kod ljudi izazove gastrointestinalne poremeć aje različitog stepena. Međutim, po navodima Svetske zdravstvene organizacije (WHO) Salmonella enterica serotip enteritidis je vodeći uzročnik salmoneloze kod ljudi tokom prethodnih 10 godina. Jedna od profilaktičkih mera je i pristupanje vakcinaciji živine protiv salmoneloze. U Srbiji je registrovano nekoliko vakcina protiv salmoneloze kod živine, različitih proizvođača. Da bi ove vakcine ušle u širu primenu u našoj zemlji, neophodno je da proizvođači u primarnoj živinarskoj proizvodnji budu upoznati sa mehanizmima delovanja tih vakcina, efektima koji se postižu njihovom primenom, ekonomskom isplativošću i moguć nošću monitoringa u skladu sa postojećom zakonskom regulativom. Svakako, vakcinaciju ne treba shvatiti kao jedini metod u profilaksi salmoneloze, već kao jedan od veoma važnih metoda u nizu biosigurnosnh i drugih mera koje bi trebalo primenjivati na farmama živine za uspešno suzbijanje salmoneloznih infekcija.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Salmonellosis in poultry breeding in view of introducing vaccination
T1  - Salmoneloza u živinarstvu u svetlu uvođenja vakcinacije
VL  - 63
IS  - 3-4
SP  - 215
EP  - 226
DO  - 10.2298/VETGL0904215R
ER  - 

@article{
author = "Resanović, Radmila and Rašić, Zoran and Kureljušić, Branislav and Vučićević, Ivana",
year = "2009",
abstract = "With the increased demand of consumers for safe poultry products, efficient control of pathogenic microorganisms that cause diseases in humans is becoming the chief challenge in contemporary poultry production. Poultry meat, eggs and related products present the main source of salmonellosis in humans that occurs as a result of the consumption of contaminated food. Any serotype of Salmonella spp. that is not strictly linked to the host is capable of causing gastrointestinal disorders of various degrees in humans. However, according to the World Health Organization (WHO), Salmonella enterica serotype enteritidis has been the leading cause of salmonellosis in humans over the past 10 years. One of the available prophylactic measures is resorting to the vaccination of poultry against salmonellosis. Several vaccines against poultry salmonellosis, produced by different manufacturers, have been registered in Serbia. In order for these vaccines to become widely used in our country, it is necessary that the factors in primary poultry production are aquainted with the mechanisms of action of these vaccines, the effects achieved by their implementation, the economic feasibility, and the possibilities for monitoring in keeping with the valid legal regulations. Certainly, vaccination should not be taken as the only method in the prophylaxis of salmonellosis, but as one of the very important methods in a series of biosafety and other measures that should be applied on poultry farms for the successful curbing of salmonellosis infections., Sa povećanim zahtevima potrošača za bezbednim živinskim proizvodima, efikasna kontrola patogenih mikroorganizama koji izazivaju oboljenja kod ljudi, postaje glavni izazov u savremenoj živinarskoj proizvodnji. Živinsko meso, jaja i proizvodi od njih predstavjaju glavni izvor salmoneloze kod ljudi koja nastaje usled konzumiranja kontaminirane hrane. Bilo koji serotip Salmonella spp. koji nije striktno vezan za domaćina sposoban je da kod ljudi izazove gastrointestinalne poremeć aje različitog stepena. Međutim, po navodima Svetske zdravstvene organizacije (WHO) Salmonella enterica serotip enteritidis je vodeći uzročnik salmoneloze kod ljudi tokom prethodnih 10 godina. Jedna od profilaktičkih mera je i pristupanje vakcinaciji živine protiv salmoneloze. U Srbiji je registrovano nekoliko vakcina protiv salmoneloze kod živine, različitih proizvođača. Da bi ove vakcine ušle u širu primenu u našoj zemlji, neophodno je da proizvođači u primarnoj živinarskoj proizvodnji budu upoznati sa mehanizmima delovanja tih vakcina, efektima koji se postižu njihovom primenom, ekonomskom isplativošću i moguć nošću monitoringa u skladu sa postojećom zakonskom regulativom. Svakako, vakcinaciju ne treba shvatiti kao jedini metod u profilaksi salmoneloze, već kao jedan od veoma važnih metoda u nizu biosigurnosnh i drugih mera koje bi trebalo primenjivati na farmama živine za uspešno suzbijanje salmoneloznih infekcija.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Salmonellosis in poultry breeding in view of introducing vaccination, Salmoneloza u živinarstvu u svetlu uvođenja vakcinacije",
volume = "63",
number = "3-4",
pages = "215-226",
doi = "10.2298/VETGL0904215R"
}

Resanović, R., Rašić, Z., Kureljušić, B.,& Vučićević, I.. (2009). Salmonellosis in poultry breeding in view of introducing vaccination. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(3-4), 215-226.
https://doi.org/10.2298/VETGL0904215R

Resanović R, Rašić Z, Kureljušić B, Vučićević I. Salmonellosis in poultry breeding in view of introducing vaccination. in Veterinarski Glasnik. 2009;63(3-4):215-226.
doi:10.2298/VETGL0904215R .

Resanović, Radmila, Rašić, Zoran, Kureljušić, Branislav, Vučićević, Ivana, "Salmonellosis in poultry breeding in view of introducing vaccination" in Veterinarski Glasnik, 63, no. 3-4 (2009):215-226,
https://doi.org/10.2298/VETGL0904215R . .

TY  - JOUR
AU  - Polaček, Vladimir
AU  - Prodanov, Jasna
AU  - Lazić, S.
AU  - Petrović, Tamaš
AU  - Rašić, Zoran
AU  - Aleksić-Kovačević, Sanja
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/471
AB  - Classical swine fever (CSF) is a highly contagious viral disease of domestic pigs and wild boars with severe consequences on animal welfare, livestock production, trade and national economy. We investigated if colostral antibodies in piglets, originating from sows vaccinated with Chine strain (C-strain) and challenged with CSF virus, affected the distribution of the B and T lymhocites in mandibular lymph nodes. Nineteen 45 days old cross breed pigs of both sexes were divided in three groups. All animals in the first group, originating from sows vaccinated with C-strain vaccine were serologically positive for the presence of specific colostral CSF virus antibodies. The second group consisted of pigs originating from unvaccinated sows serologically negative for CSF virus antibodies. Three healthy pigs serologically negative for the presence of CSF virus antibodies, originating from unvaccinated sows against CSF virus served as a control group. In the group of animals originating from unvaccinated sows against CSF, severe depletion of CD79 positive B lymphocytes was detected. In the group of pigs originating from sows vaccinated with C-strain (CSF virus antibody positive group of pigs), a reduced number of CD79 positive B lymphocytes was detected, but B cells were still present in the periphery of the germinative centres of secondary lymph follicles. There was an increase of the number of CD3 positive cells in the mandibular lymph nodes of pigs with or without maternal antibodies.
AB  - Klasična kuga svinja (KKS) je infektivno virusno oboljenje domaćih i divljih svinja, koja ima značajan uticaj kako sa stanovišta dobrobiti životinja, tako i sa stanovišta svinjarske proizvodnje, trgovine i nacionalne ekonomije. Želeli smo da ustanovimo da li specifična kolostralna antitela prasadi poreklom od krmača vakcinisanih sa Kina sojem (K soj) vakcinom protiv KKS, utiču na distribuciju B i T limfocita u mandibularnom limfnom čvoru, nakon veštačke infekcije sa virusom KKS. Devetnaest prasadi starosti 45 dana, podeljeni su u tru grupe. Jedinke iz prve grupe poticala su od krmača vakcinisanih K sojem protiv KKS i bila su serološ ki pozitivna na prisustvo specifičnih kolostralnih antitela protiv virusa KKS. Drugu grupu sačinjavala su prasad poreklom od nevakcinisanih krmača protiv KKS, koja su bila serološki negativna na prisustvo specifičnih antitela protiv virusa KKS. Tri zdrave jedinke poreklom od nevakcinisanih krmača, koja su bila serološki negativna na prisustvo antitela protiv virusa KKS predstavljale su kontrolnu grupu. U grupi životinja poreklom od nevakcinisanih krmača protiv KKS, utvrđena je deplecija B limfocita najvećeg stepena. U grupi životinja poreklom od krmača vakcinisani sa K sojem, utvrđeno je izražano smanjenje broja CD79 pozitivnih limfocita, ali su B limfociti i dalje ostali prisutni na periferiji germinitivnih centara sekundarnih limfnih folikula. Broj CD3 pozitivnih T limfocita u mandibularnim čvorovima bio je uvećan u obe grupe eksperimentalno inficiranih životinja sa virusom KKS u odnosu na kontrolnu grupu.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Immunohistochemical detection of B and T lymphocytes in mandibular lymph nodes of experimentally infected piglets with classical swine fever virus
T1  - Imunohistohemijsko dokazivanje B i T limfocita u mandibularnom limfnom čvoru prasadi eksperimentalno inficiranih virusom klasične kuge svinja
VL  - 57
IS  - 2-3
SP  - 199
EP  - 208
DO  - 10.2298/AVB0703199P
ER  - 

@article{
author = "Polaček, Vladimir and Prodanov, Jasna and Lazić, S. and Petrović, Tamaš and Rašić, Zoran and Aleksić-Kovačević, Sanja",
year = "2007",
abstract = "Classical swine fever (CSF) is a highly contagious viral disease of domestic pigs and wild boars with severe consequences on animal welfare, livestock production, trade and national economy. We investigated if colostral antibodies in piglets, originating from sows vaccinated with Chine strain (C-strain) and challenged with CSF virus, affected the distribution of the B and T lymhocites in mandibular lymph nodes. Nineteen 45 days old cross breed pigs of both sexes were divided in three groups. All animals in the first group, originating from sows vaccinated with C-strain vaccine were serologically positive for the presence of specific colostral CSF virus antibodies. The second group consisted of pigs originating from unvaccinated sows serologically negative for CSF virus antibodies. Three healthy pigs serologically negative for the presence of CSF virus antibodies, originating from unvaccinated sows against CSF virus served as a control group. In the group of animals originating from unvaccinated sows against CSF, severe depletion of CD79 positive B lymphocytes was detected. In the group of pigs originating from sows vaccinated with C-strain (CSF virus antibody positive group of pigs), a reduced number of CD79 positive B lymphocytes was detected, but B cells were still present in the periphery of the germinative centres of secondary lymph follicles. There was an increase of the number of CD3 positive cells in the mandibular lymph nodes of pigs with or without maternal antibodies., Klasična kuga svinja (KKS) je infektivno virusno oboljenje domaćih i divljih svinja, koja ima značajan uticaj kako sa stanovišta dobrobiti životinja, tako i sa stanovišta svinjarske proizvodnje, trgovine i nacionalne ekonomije. Želeli smo da ustanovimo da li specifična kolostralna antitela prasadi poreklom od krmača vakcinisanih sa Kina sojem (K soj) vakcinom protiv KKS, utiču na distribuciju B i T limfocita u mandibularnom limfnom čvoru, nakon veštačke infekcije sa virusom KKS. Devetnaest prasadi starosti 45 dana, podeljeni su u tru grupe. Jedinke iz prve grupe poticala su od krmača vakcinisanih K sojem protiv KKS i bila su serološ ki pozitivna na prisustvo specifičnih kolostralnih antitela protiv virusa KKS. Drugu grupu sačinjavala su prasad poreklom od nevakcinisanih krmača protiv KKS, koja su bila serološki negativna na prisustvo specifičnih antitela protiv virusa KKS. Tri zdrave jedinke poreklom od nevakcinisanih krmača, koja su bila serološki negativna na prisustvo antitela protiv virusa KKS predstavljale su kontrolnu grupu. U grupi životinja poreklom od nevakcinisanih krmača protiv KKS, utvrđena je deplecija B limfocita najvećeg stepena. U grupi životinja poreklom od krmača vakcinisani sa K sojem, utvrđeno je izražano smanjenje broja CD79 pozitivnih limfocita, ali su B limfociti i dalje ostali prisutni na periferiji germinitivnih centara sekundarnih limfnih folikula. Broj CD3 pozitivnih T limfocita u mandibularnim čvorovima bio je uvećan u obe grupe eksperimentalno inficiranih životinja sa virusom KKS u odnosu na kontrolnu grupu.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Immunohistochemical detection of B and T lymphocytes in mandibular lymph nodes of experimentally infected piglets with classical swine fever virus, Imunohistohemijsko dokazivanje B i T limfocita u mandibularnom limfnom čvoru prasadi eksperimentalno inficiranih virusom klasične kuge svinja",
volume = "57",
number = "2-3",
pages = "199-208",
doi = "10.2298/AVB0703199P"
}

Polaček, V., Prodanov, J., Lazić, S., Petrović, T., Rašić, Z.,& Aleksić-Kovačević, S.. (2007). Immunohistochemical detection of B and T lymphocytes in mandibular lymph nodes of experimentally infected piglets with classical swine fever virus. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(2-3), 199-208.
https://doi.org/10.2298/AVB0703199P

Polaček V, Prodanov J, Lazić S, Petrović T, Rašić Z, Aleksić-Kovačević S. Immunohistochemical detection of B and T lymphocytes in mandibular lymph nodes of experimentally infected piglets with classical swine fever virus. in Acta Veterinaria-Beograd. 2007;57(2-3):199-208.
doi:10.2298/AVB0703199P .

Polaček, Vladimir, Prodanov, Jasna, Lazić, S., Petrović, Tamaš, Rašić, Zoran, Aleksić-Kovačević, Sanja, "Immunohistochemical detection of B and T lymphocytes in mandibular lymph nodes of experimentally infected piglets with classical swine fever virus" in Acta Veterinaria-Beograd, 57, no. 2-3 (2007):199-208,
https://doi.org/10.2298/AVB0703199P . .

TY  - JOUR
AU  - Milić, Nenad
AU  - Gađanski-Omerović, Gordana
AU  - Ašanin, Ružica
AU  - Marković, B
AU  - Palić, T
AU  - Simonović, L
AU  - Rašić, Zoran
AU  - Krnjaić, Dejan
AU  - Crvak, B
AU  - Milisavljević, S
PY  - 1996
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/72
AB  - The of immunogenicity of the experimental subunit vaccine against Newcastle Disease virus was studied in a biological assay on 180, Issa Brown chickens, (80 chickens of 21 days old, and 100 chickens of 11 days old) and 50 Hybro chickens of 21 days old. The experimental subunit was prepared from purified glycoprotein subunits isolated from experimental subunit vaccine was prepared from purified glycoprotein subunits isolated from the outer envelopes of Newcastle Disease viruses, La Sota strain, which had a hemagglutinating titre of 128 HJ/0,1 ml. Antigens were adsorbed on the adjuven Al (OH)3, dissolved in 100 mmol/l PBS. Two groups of 22 and 25 experimental Issa Brown chickens, were immunized with 0,2 ml of the subunit vaccine i/m per animal, and revaccinated on day 21 of the assay. The group of 22 experimental chickens was artifically infected on day 28 after the vaccination with 200 00 EID of the virulent strain of the Newcastle Disease virus - Hertz 33 (i/m per animal). Survival percentage after the artifical infection was 95,4%. The geometric mean titres of the HI antibodies against the Newcastle Disease virus (Gmt log 2/25 ml) in the sera of the first group of 22 vaccinated chickens: were as follows: on day 7 after the vaccination 2,80, on day 21-2,70 and on day 28-4,0, and in the sera of the second group of vaccinated chickens on day 7-2,87, on day 21-2,78, and on day 28-4,17. The second two groups of 18 and 15 experimental Issa Brown chickens served as the negative and positive controls in the assay. The other part of the examination was carried out in a biologycal assay on chicken. Of the Hybro breed, 21 days old.Two groups of 16 and 14 experimental chickens immunized in the described way, were artificially infected after 21 days with 200 000 (the first group) and 400 000 (the second group) EID50 of the Hertz 33 strain (i/m per animal). Survival after the artificial infection was 94% in the first group, and 74% in the second group. Two other groups of 10 Hybro chickens served as the negative and positive controls in the assay. A comparative study of the subunit vaccine and of the TB-Mukteswar vaccine was carried out inaa biologycal assay on two groups of 50 experimental Issa chickens Brown, 11 days old. Survival after the artificial infection with 200 000 EID50 of NDV, Hertz 33 strain (i/m per animal) in the chickens vaccinated with subunit vaccine was 100% white in the chickens immunized with TB-Mukteswar vaccine survival was 80%.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Examination of the immunogenicity of experimental subunit vaccine against newcastle disease virus
VL  - 46
IS  - 5-6
SP  - 307
EP  - 315
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_72
ER  - 

@article{
author = "Milić, Nenad and Gađanski-Omerović, Gordana and Ašanin, Ružica and Marković, B and Palić, T and Simonović, L and Rašić, Zoran and Krnjaić, Dejan and Crvak, B and Milisavljević, S",
year = "1996",
abstract = "The of immunogenicity of the experimental subunit vaccine against Newcastle Disease virus was studied in a biological assay on 180, Issa Brown chickens, (80 chickens of 21 days old, and 100 chickens of 11 days old) and 50 Hybro chickens of 21 days old. The experimental subunit was prepared from purified glycoprotein subunits isolated from experimental subunit vaccine was prepared from purified glycoprotein subunits isolated from the outer envelopes of Newcastle Disease viruses, La Sota strain, which had a hemagglutinating titre of 128 HJ/0,1 ml. Antigens were adsorbed on the adjuven Al (OH)3, dissolved in 100 mmol/l PBS. Two groups of 22 and 25 experimental Issa Brown chickens, were immunized with 0,2 ml of the subunit vaccine i/m per animal, and revaccinated on day 21 of the assay. The group of 22 experimental chickens was artifically infected on day 28 after the vaccination with 200 00 EID of the virulent strain of the Newcastle Disease virus - Hertz 33 (i/m per animal). Survival percentage after the artifical infection was 95,4%. The geometric mean titres of the HI antibodies against the Newcastle Disease virus (Gmt log 2/25 ml) in the sera of the first group of 22 vaccinated chickens: were as follows: on day 7 after the vaccination 2,80, on day 21-2,70 and on day 28-4,0, and in the sera of the second group of vaccinated chickens on day 7-2,87, on day 21-2,78, and on day 28-4,17. The second two groups of 18 and 15 experimental Issa Brown chickens served as the negative and positive controls in the assay. The other part of the examination was carried out in a biologycal assay on chicken. Of the Hybro breed, 21 days old.Two groups of 16 and 14 experimental chickens immunized in the described way, were artificially infected after 21 days with 200 000 (the first group) and 400 000 (the second group) EID50 of the Hertz 33 strain (i/m per animal). Survival after the artificial infection was 94% in the first group, and 74% in the second group. Two other groups of 10 Hybro chickens served as the negative and positive controls in the assay. A comparative study of the subunit vaccine and of the TB-Mukteswar vaccine was carried out inaa biologycal assay on two groups of 50 experimental Issa chickens Brown, 11 days old. Survival after the artificial infection with 200 000 EID50 of NDV, Hertz 33 strain (i/m per animal) in the chickens vaccinated with subunit vaccine was 100% white in the chickens immunized with TB-Mukteswar vaccine survival was 80%.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Examination of the immunogenicity of experimental subunit vaccine against newcastle disease virus",
volume = "46",
number = "5-6",
pages = "307-315",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_72"
}

Milić, N., Gađanski-Omerović, G., Ašanin, R., Marković, B., Palić, T., Simonović, L., Rašić, Z., Krnjaić, D., Crvak, B.,& Milisavljević, S.. (1996). Examination of the immunogenicity of experimental subunit vaccine against newcastle disease virus. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 46(5-6), 307-315.
https://hdl.handle.net/21.15107/rcub_veterinar_72

Milić N, Gađanski-Omerović G, Ašanin R, Marković B, Palić T, Simonović L, Rašić Z, Krnjaić D, Crvak B, Milisavljević S. Examination of the immunogenicity of experimental subunit vaccine against newcastle disease virus. in Acta Veterinaria-Beograd. 1996;46(5-6):307-315.
https://hdl.handle.net/21.15107/rcub_veterinar_72 .

Milić, Nenad, Gađanski-Omerović, Gordana, Ašanin, Ružica, Marković, B, Palić, T, Simonović, L, Rašić, Zoran, Krnjaić, Dejan, Crvak, B, Milisavljević, S, "Examination of the immunogenicity of experimental subunit vaccine against newcastle disease virus" in Acta Veterinaria-Beograd, 46, no. 5-6 (1996):307-315,
https://hdl.handle.net/21.15107/rcub_veterinar_72 .