TY  - JOUR
AU  - Loncarić, Igor
AU  - Kuebber-Heiss, Anna
AU  - Posautz, Annika
AU  - Ruppitsch, Werner
AU  - Lepuschitz, Sarah
AU  - Schauer, Bernhard
AU  - Fessler, Andrea T.
AU  - Krametter-Froetscher, Reinhild
AU  - Harrison, Ewan M.
AU  - Holmes, Mark A.
AU  - Kuenzel, Frank
AU  - Szostak, Michael
AU  - Hauschild, Tomasz
AU  - Desvars-Larrive, Amelie
AU  - Mišić, Dušan
AU  - Rosengarten, Renate
AU  - Walzer, Chris
AU  - Slickers, Peter
AU  - Monecke, Stefan
AU  - Ehricht, Ralf
AU  - Schwarz, Stefan
AU  - Spergser, Joachim
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1775
AB  - The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecRI-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.
PB  - Elsevier, Amsterdam
T2  - Veterinary Microbiology
T1  - Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals
VL  - 230
SP  - 138
EP  - 144
DO  - 10.1016/j.vetmic.2019.02.014
ER  - 

@article{
author = "Loncarić, Igor and Kuebber-Heiss, Anna and Posautz, Annika and Ruppitsch, Werner and Lepuschitz, Sarah and Schauer, Bernhard and Fessler, Andrea T. and Krametter-Froetscher, Reinhild and Harrison, Ewan M. and Holmes, Mark A. and Kuenzel, Frank and Szostak, Michael and Hauschild, Tomasz and Desvars-Larrive, Amelie and Mišić, Dušan and Rosengarten, Renate and Walzer, Chris and Slickers, Peter and Monecke, Stefan and Ehricht, Ralf and Schwarz, Stefan and Spergser, Joachim",
year = "2019",
abstract = "The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecRI-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.",
publisher = "Elsevier, Amsterdam",
journal = "Veterinary Microbiology",
title = "Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals",
volume = "230",
pages = "138-144",
doi = "10.1016/j.vetmic.2019.02.014"
}

Loncarić, I., Kuebber-Heiss, A., Posautz, A., Ruppitsch, W., Lepuschitz, S., Schauer, B., Fessler, A. T., Krametter-Froetscher, R., Harrison, E. M., Holmes, M. A., Kuenzel, F., Szostak, M., Hauschild, T., Desvars-Larrive, A., Mišić, D., Rosengarten, R., Walzer, C., Slickers, P., Monecke, S., Ehricht, R., Schwarz, S.,& Spergser, J.. (2019). Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals. in Veterinary Microbiology
Elsevier, Amsterdam., 230, 138-144.
https://doi.org/10.1016/j.vetmic.2019.02.014

Loncarić I, Kuebber-Heiss A, Posautz A, Ruppitsch W, Lepuschitz S, Schauer B, Fessler AT, Krametter-Froetscher R, Harrison EM, Holmes MA, Kuenzel F, Szostak M, Hauschild T, Desvars-Larrive A, Mišić D, Rosengarten R, Walzer C, Slickers P, Monecke S, Ehricht R, Schwarz S, Spergser J. Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals. in Veterinary Microbiology. 2019;230:138-144.
doi:10.1016/j.vetmic.2019.02.014 .

Loncarić, Igor, Kuebber-Heiss, Anna, Posautz, Annika, Ruppitsch, Werner, Lepuschitz, Sarah, Schauer, Bernhard, Fessler, Andrea T., Krametter-Froetscher, Reinhild, Harrison, Ewan M., Holmes, Mark A., Kuenzel, Frank, Szostak, Michael, Hauschild, Tomasz, Desvars-Larrive, Amelie, Mišić, Dušan, Rosengarten, Renate, Walzer, Chris, Slickers, Peter, Monecke, Stefan, Ehricht, Ralf, Schwarz, Stefan, Spergser, Joachim, "Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals" in Veterinary Microbiology, 230 (2019):138-144,
https://doi.org/10.1016/j.vetmic.2019.02.014 . .

TY  - JOUR
AU  - Loncarić, Igor
AU  - Kuebber-Heiss, Anna
AU  - Posautz, Annika
AU  - Ruppitsch, Werner
AU  - Lepuschitz, Sarah
AU  - Schauer, Bernhard
AU  - Fessler, Andrea T.
AU  - Krametter-Froetscher, Reinhild
AU  - Harrison, Ewan M.
AU  - Holmes, Mark A.
AU  - Kuenzel, Frank
AU  - Szostak, Michael
AU  - Hauschild, Tomasz
AU  - Desvars-Larrive, Amelie
AU  - Mišić, Dušan
AU  - Rosengarten, Renate
AU  - Walzer, Chris
AU  - Slickers, Peter
AU  - Monecke, Stefan
AU  - Ehricht, Ralf
AU  - Schwarz, Stefan
AU  - Spergser, Joachim
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1845
AB  - The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecRI-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.
PB  - Elsevier, Amsterdam
T2  - Veterinary Microbiology
T1  - Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals
VL  - 230
SP  - 138
EP  - 144
DO  - 10.1016/j.vetmic.2019.02.014
ER  - 

@article{
author = "Loncarić, Igor and Kuebber-Heiss, Anna and Posautz, Annika and Ruppitsch, Werner and Lepuschitz, Sarah and Schauer, Bernhard and Fessler, Andrea T. and Krametter-Froetscher, Reinhild and Harrison, Ewan M. and Holmes, Mark A. and Kuenzel, Frank and Szostak, Michael and Hauschild, Tomasz and Desvars-Larrive, Amelie and Mišić, Dušan and Rosengarten, Renate and Walzer, Chris and Slickers, Peter and Monecke, Stefan and Ehricht, Ralf and Schwarz, Stefan and Spergser, Joachim",
year = "2019",
abstract = "The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecRI-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.",
publisher = "Elsevier, Amsterdam",
journal = "Veterinary Microbiology",
title = "Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals",
volume = "230",
pages = "138-144",
doi = "10.1016/j.vetmic.2019.02.014"
}

Loncarić, I., Kuebber-Heiss, A., Posautz, A., Ruppitsch, W., Lepuschitz, S., Schauer, B., Fessler, A. T., Krametter-Froetscher, R., Harrison, E. M., Holmes, M. A., Kuenzel, F., Szostak, M., Hauschild, T., Desvars-Larrive, A., Mišić, D., Rosengarten, R., Walzer, C., Slickers, P., Monecke, S., Ehricht, R., Schwarz, S.,& Spergser, J.. (2019). Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals. in Veterinary Microbiology
Elsevier, Amsterdam., 230, 138-144.
https://doi.org/10.1016/j.vetmic.2019.02.014

Loncarić I, Kuebber-Heiss A, Posautz A, Ruppitsch W, Lepuschitz S, Schauer B, Fessler AT, Krametter-Froetscher R, Harrison EM, Holmes MA, Kuenzel F, Szostak M, Hauschild T, Desvars-Larrive A, Mišić D, Rosengarten R, Walzer C, Slickers P, Monecke S, Ehricht R, Schwarz S, Spergser J. Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals. in Veterinary Microbiology. 2019;230:138-144.
doi:10.1016/j.vetmic.2019.02.014 .

Loncarić, Igor, Kuebber-Heiss, Anna, Posautz, Annika, Ruppitsch, Werner, Lepuschitz, Sarah, Schauer, Bernhard, Fessler, Andrea T., Krametter-Froetscher, Reinhild, Harrison, Ewan M., Holmes, Mark A., Kuenzel, Frank, Szostak, Michael, Hauschild, Tomasz, Desvars-Larrive, Amelie, Mišić, Dušan, Rosengarten, Renate, Walzer, Chris, Slickers, Peter, Monecke, Stefan, Ehricht, Ralf, Schwarz, Stefan, Spergser, Joachim, "Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals" in Veterinary Microbiology, 230 (2019):138-144,
https://doi.org/10.1016/j.vetmic.2019.02.014 . .

TY  - JOUR
AU  - Zuber, Ivana
AU  - Lakićević, Brankica
AU  - Pietzka, Ariane
AU  - Milanov, Dubravka
AU  - Đorđević, Vesna
AU  - Karabasil, Nedjeljko
AU  - Teodorović, Vlado
AU  - Ruppitsch, Werner
AU  - Dimitrijević, Mirjana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1844
AB  - The presence of Listeria monocytogenes was evaluated in a small-scale meat processing facility in Montenegro during 2011-2014. L. monocytogenes isolates from traditional meat products and environmental swabs were subjected to a) molecular characterization b) serotyping by both multiplex PCR and next generation sequencing (NGS) c) potential antimicrobial resistance (AMR) was assessed by extraction of specific genes from NGS data and d) screening for the presence of some disinfectant resistance markers. Overall, traditional meat products were contaminated, most likely from incoming raw materials, with 4 major specific STs of L. monocytogenes (ST515, ST8, ST21, ST121) representing 4 clonal complexes (CC1, CC8, CC21, CC121) identified during the four-year period. These strains belonged to serogroup IIa which predominated, followed by IVb (ST515, CC1). The strains from environmental swabs belonged, exclusively, to ST21 and were isolated from cutting board and floor swabs in 2011. Furthermore, we found Tn6188, a novel transposon conferring tolerance to BC, to be specific to sequence type ST121. In addition, antimicrobial resistance genes mprF and fosX were present in clonal complexes CC21 and CC121, while complexes CC8 and CC1 exclusively harbored the mprF antimicrobial resistance gene.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Food Microbiology
T1  - Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014
VL  - 79
SP  - 116
EP  - 122
DO  - 10.1016/j.fm.2018.12.005
ER  - 

@article{
author = "Zuber, Ivana and Lakićević, Brankica and Pietzka, Ariane and Milanov, Dubravka and Đorđević, Vesna and Karabasil, Nedjeljko and Teodorović, Vlado and Ruppitsch, Werner and Dimitrijević, Mirjana",
year = "2019",
abstract = "The presence of Listeria monocytogenes was evaluated in a small-scale meat processing facility in Montenegro during 2011-2014. L. monocytogenes isolates from traditional meat products and environmental swabs were subjected to a) molecular characterization b) serotyping by both multiplex PCR and next generation sequencing (NGS) c) potential antimicrobial resistance (AMR) was assessed by extraction of specific genes from NGS data and d) screening for the presence of some disinfectant resistance markers. Overall, traditional meat products were contaminated, most likely from incoming raw materials, with 4 major specific STs of L. monocytogenes (ST515, ST8, ST21, ST121) representing 4 clonal complexes (CC1, CC8, CC21, CC121) identified during the four-year period. These strains belonged to serogroup IIa which predominated, followed by IVb (ST515, CC1). The strains from environmental swabs belonged, exclusively, to ST21 and were isolated from cutting board and floor swabs in 2011. Furthermore, we found Tn6188, a novel transposon conferring tolerance to BC, to be specific to sequence type ST121. In addition, antimicrobial resistance genes mprF and fosX were present in clonal complexes CC21 and CC121, while complexes CC8 and CC1 exclusively harbored the mprF antimicrobial resistance gene.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Food Microbiology",
title = "Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014",
volume = "79",
pages = "116-122",
doi = "10.1016/j.fm.2018.12.005"
}

Zuber, I., Lakićević, B., Pietzka, A., Milanov, D., Đorđević, V., Karabasil, N., Teodorović, V., Ruppitsch, W.,& Dimitrijević, M.. (2019). Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014. in Food Microbiology
Academic Press Ltd- Elsevier Science Ltd, London., 79, 116-122.
https://doi.org/10.1016/j.fm.2018.12.005

Zuber I, Lakićević B, Pietzka A, Milanov D, Đorđević V, Karabasil N, Teodorović V, Ruppitsch W, Dimitrijević M. Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014. in Food Microbiology. 2019;79:116-122.
doi:10.1016/j.fm.2018.12.005 .

Zuber, Ivana, Lakićević, Brankica, Pietzka, Ariane, Milanov, Dubravka, Đorđević, Vesna, Karabasil, Nedjeljko, Teodorović, Vlado, Ruppitsch, Werner, Dimitrijević, Mirjana, "Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014" in Food Microbiology, 79 (2019):116-122,
https://doi.org/10.1016/j.fm.2018.12.005 . .

TY  - JOUR
AU  - Zuber, Ivana
AU  - Lakićević, Brankica
AU  - Pietzka, Ariane
AU  - Milanov, Dubravka
AU  - Đorđević, Vesna
AU  - Karabasil, Nedjeljko
AU  - Teodorović, Vlado
AU  - Ruppitsch, Werner
AU  - Dimitrijević, Mirjana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1728
AB  - The presence of Listeria monocytogenes was evaluated in a small-scale meat processing facility in Montenegro during 2011-2014. L. monocytogenes isolates from traditional meat products and environmental swabs were subjected to a) molecular characterization b) serotyping by both multiplex PCR and next generation sequencing (NGS) c) potential antimicrobial resistance (AMR) was assessed by extraction of specific genes from NGS data and d) screening for the presence of some disinfectant resistance markers. Overall, traditional meat products were contaminated, most likely from incoming raw materials, with 4 major specific STs of L. monocytogenes (ST515, ST8, ST21, ST121) representing 4 clonal complexes (CC1, CC8, CC21, CC121) identified during the four-year period. These strains belonged to serogroup IIa which predominated, followed by IVb (ST515, CC1). The strains from environmental swabs belonged, exclusively, to ST21 and were isolated from cutting board and floor swabs in 2011. Furthermore, we found Tn6188, a novel transposon conferring tolerance to BC, to be specific to sequence type ST121. In addition, antimicrobial resistance genes mprF and fosX were present in clonal complexes CC21 and CC121, while complexes CC8 and CC1 exclusively harbored the mprF antimicrobial resistance gene.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Food Microbiology
T1  - Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014
VL  - 79
SP  - 116
EP  - 122
DO  - 10.1016/j.fm.2018.12.005
ER  - 

@article{
author = "Zuber, Ivana and Lakićević, Brankica and Pietzka, Ariane and Milanov, Dubravka and Đorđević, Vesna and Karabasil, Nedjeljko and Teodorović, Vlado and Ruppitsch, Werner and Dimitrijević, Mirjana",
year = "2019",
abstract = "The presence of Listeria monocytogenes was evaluated in a small-scale meat processing facility in Montenegro during 2011-2014. L. monocytogenes isolates from traditional meat products and environmental swabs were subjected to a) molecular characterization b) serotyping by both multiplex PCR and next generation sequencing (NGS) c) potential antimicrobial resistance (AMR) was assessed by extraction of specific genes from NGS data and d) screening for the presence of some disinfectant resistance markers. Overall, traditional meat products were contaminated, most likely from incoming raw materials, with 4 major specific STs of L. monocytogenes (ST515, ST8, ST21, ST121) representing 4 clonal complexes (CC1, CC8, CC21, CC121) identified during the four-year period. These strains belonged to serogroup IIa which predominated, followed by IVb (ST515, CC1). The strains from environmental swabs belonged, exclusively, to ST21 and were isolated from cutting board and floor swabs in 2011. Furthermore, we found Tn6188, a novel transposon conferring tolerance to BC, to be specific to sequence type ST121. In addition, antimicrobial resistance genes mprF and fosX were present in clonal complexes CC21 and CC121, while complexes CC8 and CC1 exclusively harbored the mprF antimicrobial resistance gene.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Food Microbiology",
title = "Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014",
volume = "79",
pages = "116-122",
doi = "10.1016/j.fm.2018.12.005"
}

Zuber, I., Lakićević, B., Pietzka, A., Milanov, D., Đorđević, V., Karabasil, N., Teodorović, V., Ruppitsch, W.,& Dimitrijević, M.. (2019). Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014. in Food Microbiology
Academic Press Ltd- Elsevier Science Ltd, London., 79, 116-122.
https://doi.org/10.1016/j.fm.2018.12.005

Zuber I, Lakićević B, Pietzka A, Milanov D, Đorđević V, Karabasil N, Teodorović V, Ruppitsch W, Dimitrijević M. Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014. in Food Microbiology. 2019;79:116-122.
doi:10.1016/j.fm.2018.12.005 .

Zuber, Ivana, Lakićević, Brankica, Pietzka, Ariane, Milanov, Dubravka, Đorđević, Vesna, Karabasil, Nedjeljko, Teodorović, Vlado, Ruppitsch, Werner, Dimitrijević, Mirjana, "Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014" in Food Microbiology, 79 (2019):116-122,
https://doi.org/10.1016/j.fm.2018.12.005 . .