TY  - JOUR
AU  - Tubić Vukajlović, Jovana
AU  - Kosanić, Marijana
AU  - Ranković, Branislav
AU  - Stanojković, Tatjana
AU  - Marković, Aleksandra
AU  - Grujičić, Darko
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Milošević-Djordjević, Olivera
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2318
AB  - Bioactivity of acetone extract of Leccinum scabrum was evaluated. Antioxidant activity was evaluated by free radical scavenging activity, reducing power and determination of total phenolic content. The microdilution plate method was used to detect antimicrobial activity. Cytotoxic activity was tested by MTT assay. Genotoxic potential was evaluated by comet assay. Results showed a stronger free radical scavenging activity, but the reducing power was less pronounced. The total content of phenols in the extract was 14.91 µg PE/mg. The minimum inhibitory concentration related to the tested microorganisms fluctuated in the range 0.039-10 mg/mL. The most sensitive was Bacillus cereus. A strong cytotoxic activity was revealed with IC50 values ranging between 73.64 and 200.00 µg/mL, among which HeLa was the most sensitive. Results showed that the extract was not genotoxic and it decreased the H2O2-induced GDI values in comparison to the positive control. L. scabrum possesses bioactivities such as antioxidant, antimicrobial, cytotoxic, no genotoxic and protective against H2O2, and in the highest tested concentration it showed a synergistic activity.
AB  - Studiul prezintă evaluarea activității biologice a extractului în acetonă obținut din Leccinum scabrum. Activitatea antioxidantă a fost evaluată prin determinarea capacității de chelatare a radicalilor liberi, a capacității reducătoare și a conținutului total în fenoli. Activitatea antimicrobiană a fost testată prin metoda microdiluției, activitatea citotoxică prin metoda MTT, iar potențialul genotoxic a fost determinat prin calcularea indexului de deteriorare genetică. Conținutul total de fenoli a fost 14,91 μg PE/mg. Concentrația minimă inhibitorie a variat în intervalul 0,039 - 10 mg/mL. Activitatea citotoxică pronunțată a fost corelată cu valorile IC50 în domeniul 73,64 - 200,00 μg/mL. Rezultatele au arătat că extractul nu este genotoxic și au scăzut valorile indexului de deteriorare genetică-indus de H2O2 , în comparație cu controlul pozitiv. L. scabrum posedă activități antioxidantă, antimicrobiană, citotoxică șı protectoare împotriva H2O2.
PB  - Societatea de Științe Farmaceutice din România
T2  - Farmacia
T1  - Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum
VL  - 69
IS  - 5
SP  - 974
EP  - 979
DO  - 10.31925/FARMACIA.2021.5.22
ER  - 

@article{
author = "Tubić Vukajlović, Jovana and Kosanić, Marijana and Ranković, Branislav and Stanojković, Tatjana and Marković, Aleksandra and Grujičić, Darko and Đelić, Ninoslav and Radaković, Milena and Milošević-Djordjević, Olivera",
year = "2021",
abstract = "Bioactivity of acetone extract of Leccinum scabrum was evaluated. Antioxidant activity was evaluated by free radical scavenging activity, reducing power and determination of total phenolic content. The microdilution plate method was used to detect antimicrobial activity. Cytotoxic activity was tested by MTT assay. Genotoxic potential was evaluated by comet assay. Results showed a stronger free radical scavenging activity, but the reducing power was less pronounced. The total content of phenols in the extract was 14.91 µg PE/mg. The minimum inhibitory concentration related to the tested microorganisms fluctuated in the range 0.039-10 mg/mL. The most sensitive was Bacillus cereus. A strong cytotoxic activity was revealed with IC50 values ranging between 73.64 and 200.00 µg/mL, among which HeLa was the most sensitive. Results showed that the extract was not genotoxic and it decreased the H2O2-induced GDI values in comparison to the positive control. L. scabrum possesses bioactivities such as antioxidant, antimicrobial, cytotoxic, no genotoxic and protective against H2O2, and in the highest tested concentration it showed a synergistic activity., Studiul prezintă evaluarea activității biologice a extractului în acetonă obținut din Leccinum scabrum. Activitatea antioxidantă a fost evaluată prin determinarea capacității de chelatare a radicalilor liberi, a capacității reducătoare și a conținutului total în fenoli. Activitatea antimicrobiană a fost testată prin metoda microdiluției, activitatea citotoxică prin metoda MTT, iar potențialul genotoxic a fost determinat prin calcularea indexului de deteriorare genetică. Conținutul total de fenoli a fost 14,91 μg PE/mg. Concentrația minimă inhibitorie a variat în intervalul 0,039 - 10 mg/mL. Activitatea citotoxică pronunțată a fost corelată cu valorile IC50 în domeniul 73,64 - 200,00 μg/mL. Rezultatele au arătat că extractul nu este genotoxic și au scăzut valorile indexului de deteriorare genetică-indus de H2O2 , în comparație cu controlul pozitiv. L. scabrum posedă activități antioxidantă, antimicrobiană, citotoxică șı protectoare împotriva H2O2.",
publisher = "Societatea de Științe Farmaceutice din România",
journal = "Farmacia",
title = "Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum",
volume = "69",
number = "5",
pages = "974-979",
doi = "10.31925/FARMACIA.2021.5.22"
}

Tubić Vukajlović, J., Kosanić, M., Ranković, B., Stanojković, T., Marković, A., Grujičić, D., Đelić, N., Radaković, M.,& Milošević-Djordjević, O.. (2021). Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum. in Farmacia
Societatea de Științe Farmaceutice din România., 69(5), 974-979.
https://doi.org/10.31925/FARMACIA.2021.5.22

Tubić Vukajlović J, Kosanić M, Ranković B, Stanojković T, Marković A, Grujičić D, Đelić N, Radaković M, Milošević-Djordjević O. Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum. in Farmacia. 2021;69(5):974-979.
doi:10.31925/FARMACIA.2021.5.22 .

Tubić Vukajlović, Jovana, Kosanić, Marijana, Ranković, Branislav, Stanojković, Tatjana, Marković, Aleksandra, Grujičić, Darko, Đelić, Ninoslav, Radaković, Milena, Milošević-Djordjević, Olivera, "Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum" in Farmacia, 69, no. 5 (2021):974-979,
https://doi.org/10.31925/FARMACIA.2021.5.22 . .

TY  - JOUR
AU  - Cenić-Milošević, Desanka
AU  - Tambur, Zoran
AU  - Ivancajić, S.
AU  - Stanojković, Tatjana
AU  - Grozdanić, Nadja
AU  - Kulišić, Zoran
AU  - Juranić, Zorica
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1084
AB  - Tanaceti partheni, Hypericum perforatum and propolis have been widely used for centuries and are well-documented medicinal plants and natural product. In this study, we investigated the antiproliferative effects of water extracts of ethanolic dry extracts of two different medicinal plants (Tanaceti partheni and Hypericum perforatum) and propolis on HeLa cells. The Tanaceti partheni extract exhibited mild cytotoxic activity The IC50 was 153.71 mu g/mL. The extract of Hypericum perforatum did not show active cytotoxic activity against HeLa cells (IC50 >200 [mu g/mL). Regarding the antiproliferative effects of Hypericum perforatum, our results are not in correlation with the results of other authors, probably because different Hypericum species and different human cancer cell lines were used. The extract of propolis did not show active cytotoxic activity against HeLa cells (IC50 +/- 1.08 0.01 mg/mL). The weak antiproliferative effect of propolis on HeLa cells is either due to the use of a low concentration of propolis extracted in weakly polar solvents, or the use of propolis collected in the autumn.
PB  - Srpsko biološko društvo, Beograd
T2  - Archives of Biological Sciences
T1  - Antiproliferative effects of tanaceti partheni, hypericum perforatum and propolis on hela cells
VL  - 66
IS  - 2
SP  - 705
EP  - 712
DO  - 10.2298/ABS1402705M
ER  - 

@article{
author = "Cenić-Milošević, Desanka and Tambur, Zoran and Ivancajić, S. and Stanojković, Tatjana and Grozdanić, Nadja and Kulišić, Zoran and Juranić, Zorica",
year = "2014",
abstract = "Tanaceti partheni, Hypericum perforatum and propolis have been widely used for centuries and are well-documented medicinal plants and natural product. In this study, we investigated the antiproliferative effects of water extracts of ethanolic dry extracts of two different medicinal plants (Tanaceti partheni and Hypericum perforatum) and propolis on HeLa cells. The Tanaceti partheni extract exhibited mild cytotoxic activity The IC50 was 153.71 mu g/mL. The extract of Hypericum perforatum did not show active cytotoxic activity against HeLa cells (IC50 >200 [mu g/mL). Regarding the antiproliferative effects of Hypericum perforatum, our results are not in correlation with the results of other authors, probably because different Hypericum species and different human cancer cell lines were used. The extract of propolis did not show active cytotoxic activity against HeLa cells (IC50 +/- 1.08 0.01 mg/mL). The weak antiproliferative effect of propolis on HeLa cells is either due to the use of a low concentration of propolis extracted in weakly polar solvents, or the use of propolis collected in the autumn.",
publisher = "Srpsko biološko društvo, Beograd",
journal = "Archives of Biological Sciences",
title = "Antiproliferative effects of tanaceti partheni, hypericum perforatum and propolis on hela cells",
volume = "66",
number = "2",
pages = "705-712",
doi = "10.2298/ABS1402705M"
}

Cenić-Milošević, D., Tambur, Z., Ivancajić, S., Stanojković, T., Grozdanić, N., Kulišić, Z.,& Juranić, Z.. (2014). Antiproliferative effects of tanaceti partheni, hypericum perforatum and propolis on hela cells. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd., 66(2), 705-712.
https://doi.org/10.2298/ABS1402705M

Cenić-Milošević D, Tambur Z, Ivancajić S, Stanojković T, Grozdanić N, Kulišić Z, Juranić Z. Antiproliferative effects of tanaceti partheni, hypericum perforatum and propolis on hela cells. in Archives of Biological Sciences. 2014;66(2):705-712.
doi:10.2298/ABS1402705M .

Cenić-Milošević, Desanka, Tambur, Zoran, Ivancajić, S., Stanojković, Tatjana, Grozdanić, Nadja, Kulišić, Zoran, Juranić, Zorica, "Antiproliferative effects of tanaceti partheni, hypericum perforatum and propolis on hela cells" in Archives of Biological Sciences, 66, no. 2 (2014):705-712,
https://doi.org/10.2298/ABS1402705M . .

TY  - JOUR
AU  - Cenić-Milošević, Desanka
AU  - Tambur, Zoran
AU  - Ivančajić, S.
AU  - Bokonjić, Dubravko
AU  - Čuković, Anika
AU  - Stanojković, Tatjana
AU  - Grozdanić, Nadja
AU  - Kulišić, Zoran
AU  - Juranić, Zorica
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/965
AB  - The use of medicinal plants is becoming increasingly appreciated in suppressing cancer growth and cancer prevention. In this study the antiproliferative effects of the water extracts of previously obtained ethanolic dry extracts of three different medicinal plants (Camellia sinensis, Frangula alnus from two different places and Rosmarinus officinalis) using cell lines derived from human cervix adenocarcinoma (Hela cells) were investigated. The extract of Camellia sinensis exhibited significant cytotoxic effect against Hela cells (IC50 40.88 μg/ml). Screening in Hela cells revealed a moderate cytotoxic effect (IC50 80.26μg/ml) of the extract of Rosmarinus officinalis, a mild cytotoxic effect (IC50 148.05 μg/ml) of the extract of Frangula alnus (originating in Bosnia), and the extract of Frangula alnus (originating in Serbia) did not show active cytotoxicity (IC50 > 200 μg/ml). The best antiproliferative properties are those of Camellia sinensis, followed by Rosmarinus officinalis, and the least effective was Frangula alnus. As regards geographic origin, the Frangula alnus from Bosnia possessed better antiproliferative effects than Frangula alnus from Serbia.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Antiproliferative effects of Camellia sinensis, Frangula alnus and Rosmarinus officinalis
VL  - 65
IS  - 3
SP  - 885
EP  - 891
DO  - 10.2298/ABS1303885M
ER  - 

@article{
author = "Cenić-Milošević, Desanka and Tambur, Zoran and Ivančajić, S. and Bokonjić, Dubravko and Čuković, Anika and Stanojković, Tatjana and Grozdanić, Nadja and Kulišić, Zoran and Juranić, Zorica",
year = "2013",
abstract = "The use of medicinal plants is becoming increasingly appreciated in suppressing cancer growth and cancer prevention. In this study the antiproliferative effects of the water extracts of previously obtained ethanolic dry extracts of three different medicinal plants (Camellia sinensis, Frangula alnus from two different places and Rosmarinus officinalis) using cell lines derived from human cervix adenocarcinoma (Hela cells) were investigated. The extract of Camellia sinensis exhibited significant cytotoxic effect against Hela cells (IC50 40.88 μg/ml). Screening in Hela cells revealed a moderate cytotoxic effect (IC50 80.26μg/ml) of the extract of Rosmarinus officinalis, a mild cytotoxic effect (IC50 148.05 μg/ml) of the extract of Frangula alnus (originating in Bosnia), and the extract of Frangula alnus (originating in Serbia) did not show active cytotoxicity (IC50 > 200 μg/ml). The best antiproliferative properties are those of Camellia sinensis, followed by Rosmarinus officinalis, and the least effective was Frangula alnus. As regards geographic origin, the Frangula alnus from Bosnia possessed better antiproliferative effects than Frangula alnus from Serbia.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Antiproliferative effects of Camellia sinensis, Frangula alnus and Rosmarinus officinalis",
volume = "65",
number = "3",
pages = "885-891",
doi = "10.2298/ABS1303885M"
}

Cenić-Milošević, D., Tambur, Z., Ivančajić, S., Bokonjić, D., Čuković, A., Stanojković, T., Grozdanić, N., Kulišić, Z.,& Juranić, Z.. (2013). Antiproliferative effects of Camellia sinensis, Frangula alnus and Rosmarinus officinalis. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 65(3), 885-891.
https://doi.org/10.2298/ABS1303885M

Cenić-Milošević D, Tambur Z, Ivančajić S, Bokonjić D, Čuković A, Stanojković T, Grozdanić N, Kulišić Z, Juranić Z. Antiproliferative effects of Camellia sinensis, Frangula alnus and Rosmarinus officinalis. in Archives of Biological Sciences. 2013;65(3):885-891.
doi:10.2298/ABS1303885M .

Cenić-Milošević, Desanka, Tambur, Zoran, Ivančajić, S., Bokonjić, Dubravko, Čuković, Anika, Stanojković, Tatjana, Grozdanić, Nadja, Kulišić, Zoran, Juranić, Zorica, "Antiproliferative effects of Camellia sinensis, Frangula alnus and Rosmarinus officinalis" in Archives of Biological Sciences, 65, no. 3 (2013):885-891,
https://doi.org/10.2298/ABS1303885M . .