TY  - JOUR
AU  - Nišavić, Jakov
AU  - Radalj, Andrea
AU  - Milić, Nenad
AU  - Živulj, Aleksandar
AU  - Benković, Damir
AU  - Stanojković, Aleksandar
AU  - Prošić, Isidora
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2339
AB  - Wild boars are one of the widest-ranging mammals worldwide and represent reservoirs for many important viruses. Disease outbreaks in domestic swine are often described as a consequence of contact with wild boars, and traditional rearing conditions are a particular risk factor. Examples of such diseases include classical swine fever (CSF), African swine fever (ASF), Aujeszky’s disease (AD), and diseases caused by porcine circoviruses and parvoviruses. Some viral infections causing high mortality rates are easily noticeable and thus reported, though many viruses infecting wildlife are insidious impacting survival rates and reproduction in wild animals. Samples from wild boars for laboratory testing are usually collected postmortem and include various tissues or blood sera. The recovery of viable viruses during virus isolation depends on the virus species and the condition of the sample. Since this method does not yield timely results, most diagnostic procedures are based on PCR or antigen detection methods. Serological surveys are inexpensive and appropriate for prevalence studies. When interpreting the results of diagnostic tests, both virus and host characteristics, and the epizootiological situation must be accounted for. Disease control techniques such as fencing or feeding wild boars cause animal aggregation and give rise to population density which favors pathogen maintenance in the environment. Hunting reduces the number of susceptible animals and is helpful as an additional control measure and for sampling. Available data on infectious disease dynamics in wild boars is scarce, and constant knowledge improvement on pathogenesis, clinical symptoms, risk factors, and adequate control measures are required.
AB  - Divlje svinje su jedna on najrasprostranjenijih vrsta sisara na planeti, a ujedno predstavljaju i rezervoare mnogih značajnih virusa. Pojava oboljenja u populacijama domaćih svinja se javlja kao posledica kontakta sa divljim svinjama pri čemu tradicionalan način uzgoja životinja predstavlja faktor rizika. Primeri takvih obljenja su: klasična kuga svinja, afrička kuga svinja, Aujeckijeva bolest i oboljenja izazvana svinjskim cirkovirusima i parvovirusima. Određene virusne infekcije sa visokom stopom mortaliteta se mogu lako detektovati, a samim tim i prijaviti, međutim neki virusi divljih svinja ne dovode do vidljivih promena što otežava njihovo otkrivanje. Uzorci za laboratorijska ispitivanja poreklom od divljih svinja se najčešće prikupljaju postmortalno i uključuju različita tkiva ili krvni serum. Uspešnost izolacije virusa u kulturi ćelija zavisi od vrste virusa kao i od stanja dostavljenog uzorka. S obzirom da primena navedene metode oduzima vreme, većina procedura se zasniva na PCR ili metodama detekcije antigena. Pored toga, serološke metode su ekonomski isplative i pogodne za izvođenje studija prevalencije. Prilikom interpretacije rezultata laboratorijskih analiza je izuzetno značajno uzeti u obzir više parametara uključujući osobine virusa i domaćina kao i epizootiološku situaciju na terenu. Metode kontrole zaraznih bolesti divljih svinja poput ograđivanja ili dohranjivanja životinja dovode do povećanja gustine populacije što pogoduje transmisiji patogena. Lov dovodi do smanjenja broja osetljivih životinja u određenoj sredini, međutim koristan je kao dodatna mera kontrole i omogućuje prikupljanje uzoraka. Dostupni podaci o dinamici infektivnih oboljenja divljih svinja su ograničeni i neophodno je konstantno izučavanje njihove patogeneze, kliničkih osobenosti, faktora rizika kao i procena primene određenih mera kontrole.
PB  - Institute for Animal Husbandry
T2  - Biotechnology in Animal Husbandry
T1  - A review of some important viral diseases of wild boars
VL  - 37
IS  - 4
SP  - 235
EP  - 254
DO  - 10.2298/BAH2104235N
ER  - 

@article{
author = "Nišavić, Jakov and Radalj, Andrea and Milić, Nenad and Živulj, Aleksandar and Benković, Damir and Stanojković, Aleksandar and Prošić, Isidora",
year = "2021",
abstract = "Wild boars are one of the widest-ranging mammals worldwide and represent reservoirs for many important viruses. Disease outbreaks in domestic swine are often described as a consequence of contact with wild boars, and traditional rearing conditions are a particular risk factor. Examples of such diseases include classical swine fever (CSF), African swine fever (ASF), Aujeszky’s disease (AD), and diseases caused by porcine circoviruses and parvoviruses. Some viral infections causing high mortality rates are easily noticeable and thus reported, though many viruses infecting wildlife are insidious impacting survival rates and reproduction in wild animals. Samples from wild boars for laboratory testing are usually collected postmortem and include various tissues or blood sera. The recovery of viable viruses during virus isolation depends on the virus species and the condition of the sample. Since this method does not yield timely results, most diagnostic procedures are based on PCR or antigen detection methods. Serological surveys are inexpensive and appropriate for prevalence studies. When interpreting the results of diagnostic tests, both virus and host characteristics, and the epizootiological situation must be accounted for. Disease control techniques such as fencing or feeding wild boars cause animal aggregation and give rise to population density which favors pathogen maintenance in the environment. Hunting reduces the number of susceptible animals and is helpful as an additional control measure and for sampling. Available data on infectious disease dynamics in wild boars is scarce, and constant knowledge improvement on pathogenesis, clinical symptoms, risk factors, and adequate control measures are required., Divlje svinje su jedna on najrasprostranjenijih vrsta sisara na planeti, a ujedno predstavljaju i rezervoare mnogih značajnih virusa. Pojava oboljenja u populacijama domaćih svinja se javlja kao posledica kontakta sa divljim svinjama pri čemu tradicionalan način uzgoja životinja predstavlja faktor rizika. Primeri takvih obljenja su: klasična kuga svinja, afrička kuga svinja, Aujeckijeva bolest i oboljenja izazvana svinjskim cirkovirusima i parvovirusima. Određene virusne infekcije sa visokom stopom mortaliteta se mogu lako detektovati, a samim tim i prijaviti, međutim neki virusi divljih svinja ne dovode do vidljivih promena što otežava njihovo otkrivanje. Uzorci za laboratorijska ispitivanja poreklom od divljih svinja se najčešće prikupljaju postmortalno i uključuju različita tkiva ili krvni serum. Uspešnost izolacije virusa u kulturi ćelija zavisi od vrste virusa kao i od stanja dostavljenog uzorka. S obzirom da primena navedene metode oduzima vreme, većina procedura se zasniva na PCR ili metodama detekcije antigena. Pored toga, serološke metode su ekonomski isplative i pogodne za izvođenje studija prevalencije. Prilikom interpretacije rezultata laboratorijskih analiza je izuzetno značajno uzeti u obzir više parametara uključujući osobine virusa i domaćina kao i epizootiološku situaciju na terenu. Metode kontrole zaraznih bolesti divljih svinja poput ograđivanja ili dohranjivanja životinja dovode do povećanja gustine populacije što pogoduje transmisiji patogena. Lov dovodi do smanjenja broja osetljivih životinja u određenoj sredini, međutim koristan je kao dodatna mera kontrole i omogućuje prikupljanje uzoraka. Dostupni podaci o dinamici infektivnih oboljenja divljih svinja su ograničeni i neophodno je konstantno izučavanje njihove patogeneze, kliničkih osobenosti, faktora rizika kao i procena primene određenih mera kontrole.",
publisher = "Institute for Animal Husbandry",
journal = "Biotechnology in Animal Husbandry",
title = "A review of some important viral diseases of wild boars",
volume = "37",
number = "4",
pages = "235-254",
doi = "10.2298/BAH2104235N"
}

Nišavić, J., Radalj, A., Milić, N., Živulj, A., Benković, D., Stanojković, A.,& Prošić, I.. (2021). A review of some important viral diseases of wild boars. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry., 37(4), 235-254.
https://doi.org/10.2298/BAH2104235N

Nišavić J, Radalj A, Milić N, Živulj A, Benković D, Stanojković A, Prošić I. A review of some important viral diseases of wild boars. in Biotechnology in Animal Husbandry. 2021;37(4):235-254.
doi:10.2298/BAH2104235N .

Nišavić, Jakov, Radalj, Andrea, Milić, Nenad, Živulj, Aleksandar, Benković, Damir, Stanojković, Aleksandar, Prošić, Isidora, "A review of some important viral diseases of wild boars" in Biotechnology in Animal Husbandry, 37, no. 4 (2021):235-254,
https://doi.org/10.2298/BAH2104235N . .

TY  - JOUR
AU  - Nišavić, Jakov
AU  - Milić, Nenad
AU  - Radalj, Andrea
AU  - Stanojković, Aleksandar
AU  - Veljović, Ljubiša
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2260
AB  - The bovine respiratory disease complex (BRDC) is multifactorial and results from interactions between host factors, environmental factors, and pathogens. A virus, as an initial pathogen alters the animal’s immunity supporting the bacterial colonization of the lower respiratory tract. Bovine herpesvirus 1 (BHV-1), bovine parainfluenza virus 3 (BPIV-3), and bovine respiratory syncytial virus (BRSV) are among the most significant viruses associated with BRDC. The disease most often affects young and older immunosuppressed animals. Laboratory results depend on the selected sampling site of the respiratory tract and proper timing during the period of virus shedding. The samples for testing mostly include nasal or nasopharyngeal swabs, tracheal wash, bronchoalveolar lavage fluid, or necropsy specimens. Virus isolation, although considered as the gold standard, is time-consuming and depends on the virus species and sampling conditions. Most of the virus identification methods used today are molecular assays (conventional and real-time PCR or RT-PCR) that are rapid, sensitive, and specific, which is of the essence in veterinary diagnostic laboratories. DNA sequencing is mostly used to detect specific genetic mutations and for molecular epidemiology of disease outbreaks. Serological diagnosis is performed based on the detection of specific antibody presence after infection of seronegative animals or a 4-fold specific antibody titer rise in paired serum samples. Different assays are available, including virus neutralization, complement fixation, haemagglutination inhibition, and ELISA. The early and reliable diagnosis is beneficial in the management and control of BRDC and is the basis of a timely treatment and prevention program.
PB  - Belgrade : Institute for Animal Husbandry
T2  - Biotechnology in Animal Husbandry
T1  - Laboratory diagnostics of bovine parainfluenza-3 virus, bovine herpesvirus 1, and bovine respiratory syncytial virus associated with bovine respiratory disease
T1  - Laboratorijska dijagnostika virusa parainfluence 3 goveda, goveđeg herpesvirusa 1 i goveđeg respiratornog sincicijalnog virusa kao uzročnika respiratornog sindroma goveda
VL  - 37
IS  - 1
SP  - 1
EP  - 15
DO  - 10.2298/BAH2101001N
ER  - 

@article{
author = "Nišavić, Jakov and Milić, Nenad and Radalj, Andrea and Stanojković, Aleksandar and Veljović, Ljubiša",
year = "2021",
abstract = "The bovine respiratory disease complex (BRDC) is multifactorial and results from interactions between host factors, environmental factors, and pathogens. A virus, as an initial pathogen alters the animal’s immunity supporting the bacterial colonization of the lower respiratory tract. Bovine herpesvirus 1 (BHV-1), bovine parainfluenza virus 3 (BPIV-3), and bovine respiratory syncytial virus (BRSV) are among the most significant viruses associated with BRDC. The disease most often affects young and older immunosuppressed animals. Laboratory results depend on the selected sampling site of the respiratory tract and proper timing during the period of virus shedding. The samples for testing mostly include nasal or nasopharyngeal swabs, tracheal wash, bronchoalveolar lavage fluid, or necropsy specimens. Virus isolation, although considered as the gold standard, is time-consuming and depends on the virus species and sampling conditions. Most of the virus identification methods used today are molecular assays (conventional and real-time PCR or RT-PCR) that are rapid, sensitive, and specific, which is of the essence in veterinary diagnostic laboratories. DNA sequencing is mostly used to detect specific genetic mutations and for molecular epidemiology of disease outbreaks. Serological diagnosis is performed based on the detection of specific antibody presence after infection of seronegative animals or a 4-fold specific antibody titer rise in paired serum samples. Different assays are available, including virus neutralization, complement fixation, haemagglutination inhibition, and ELISA. The early and reliable diagnosis is beneficial in the management and control of BRDC and is the basis of a timely treatment and prevention program.",
publisher = "Belgrade : Institute for Animal Husbandry",
journal = "Biotechnology in Animal Husbandry",
title = "Laboratory diagnostics of bovine parainfluenza-3 virus, bovine herpesvirus 1, and bovine respiratory syncytial virus associated with bovine respiratory disease, Laboratorijska dijagnostika virusa parainfluence 3 goveda, goveđeg herpesvirusa 1 i goveđeg respiratornog sincicijalnog virusa kao uzročnika respiratornog sindroma goveda",
volume = "37",
number = "1",
pages = "1-15",
doi = "10.2298/BAH2101001N"
}

Nišavić, J., Milić, N., Radalj, A., Stanojković, A.,& Veljović, L.. (2021). Laboratory diagnostics of bovine parainfluenza-3 virus, bovine herpesvirus 1, and bovine respiratory syncytial virus associated with bovine respiratory disease. in Biotechnology in Animal Husbandry
Belgrade : Institute for Animal Husbandry., 37(1), 1-15.
https://doi.org/10.2298/BAH2101001N

Nišavić J, Milić N, Radalj A, Stanojković A, Veljović L. Laboratory diagnostics of bovine parainfluenza-3 virus, bovine herpesvirus 1, and bovine respiratory syncytial virus associated with bovine respiratory disease. in Biotechnology in Animal Husbandry. 2021;37(1):1-15.
doi:10.2298/BAH2101001N .

Nišavić, Jakov, Milić, Nenad, Radalj, Andrea, Stanojković, Aleksandar, Veljović, Ljubiša, "Laboratory diagnostics of bovine parainfluenza-3 virus, bovine herpesvirus 1, and bovine respiratory syncytial virus associated with bovine respiratory disease" in Biotechnology in Animal Husbandry, 37, no. 1 (2021):1-15,
https://doi.org/10.2298/BAH2101001N . .

TY  - JOUR
AU  - Tarić, Elmin
AU  - Glavinić, Uroš
AU  - Vejnović, Branislav
AU  - Stanojković, Aleksandar
AU  - Aleksić, Nevenka
AU  - Dimitrijević, Vladimir
AU  - Stanimirović, Zoran
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1838
AB  - Commercially and traditionally managed bees were compared for oxidative stress (superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and malondialdehyde (MDA)), the prevalence of parasites (<i>Lotmaria passim</i>, <i>Crithidia mellificae</i> and <i>Nosema ceranae/apis</i>) and social immunity (glucose oxidase gene expression). The research was conducted on Pester plateau (Serbia—the Balkan Peninsula), on seemingly healthy colonies. Significant differences in CAT, GST and SOD activities (<i>p</i> < 0.01), and MDA concentrations (<i>p</i> < 0.002) were detected between commercial and traditional colonies. In the former, the prevalence of both <i>L. passim</i> and <i>N. ceranae</i> was significantly (<i>p</i> < 0.05 and <i>p</i> < 0.01, respectively) higher. For the first time, <i>L. passim</i> was detected in honey bee brood. In commercial colonies, the prevalence of <i>L. passim</i> was significantly (<i>p</i> < 0.01) lower in brood than in adult bees, whilst in traditionally kept colonies the prevalence in adult bees and brood did not differ significantly. In commercially kept colonies, the GOX gene expression level was significantly (<i>p</i> < 0.01) higher, which probably results from their increased need to strengthen their social immunity. Commercially kept colonies were under higher oxidative stress, had higher parasite burdens and higher GOX gene transcript levels. It may be assumed that anthropogenic influence contributed to these differences, but further investigations are necessary to confirm that.
PB  - MDPI
T2  - Insects
T1  - Oxidative Stress, Endoparasite Prevalence and Social Immunity in Bee Colonies Kept Traditionally vs. Those Kept for Commercial Purposes
VL  - 11
IS  - 266
SP  - 266
DO  - 10.3390/insects11050266
ER  - 

@article{
author = "Tarić, Elmin and Glavinić, Uroš and Vejnović, Branislav and Stanojković, Aleksandar and Aleksić, Nevenka and Dimitrijević, Vladimir and Stanimirović, Zoran",
year = "2020",
abstract = "Commercially and traditionally managed bees were compared for oxidative stress (superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and malondialdehyde (MDA)), the prevalence of parasites (<i>Lotmaria passim</i>, <i>Crithidia mellificae</i> and <i>Nosema ceranae/apis</i>) and social immunity (glucose oxidase gene expression). The research was conducted on Pester plateau (Serbia—the Balkan Peninsula), on seemingly healthy colonies. Significant differences in CAT, GST and SOD activities (<i>p</i> < 0.01), and MDA concentrations (<i>p</i> < 0.002) were detected between commercial and traditional colonies. In the former, the prevalence of both <i>L. passim</i> and <i>N. ceranae</i> was significantly (<i>p</i> < 0.05 and <i>p</i> < 0.01, respectively) higher. For the first time, <i>L. passim</i> was detected in honey bee brood. In commercial colonies, the prevalence of <i>L. passim</i> was significantly (<i>p</i> < 0.01) lower in brood than in adult bees, whilst in traditionally kept colonies the prevalence in adult bees and brood did not differ significantly. In commercially kept colonies, the GOX gene expression level was significantly (<i>p</i> < 0.01) higher, which probably results from their increased need to strengthen their social immunity. Commercially kept colonies were under higher oxidative stress, had higher parasite burdens and higher GOX gene transcript levels. It may be assumed that anthropogenic influence contributed to these differences, but further investigations are necessary to confirm that.",
publisher = "MDPI",
journal = "Insects",
title = "Oxidative Stress, Endoparasite Prevalence and Social Immunity in Bee Colonies Kept Traditionally vs. Those Kept for Commercial Purposes",
volume = "11",
number = "266",
pages = "266",
doi = "10.3390/insects11050266"
}

Tarić, E., Glavinić, U., Vejnović, B., Stanojković, A., Aleksić, N., Dimitrijević, V.,& Stanimirović, Z.. (2020). Oxidative Stress, Endoparasite Prevalence and Social Immunity in Bee Colonies Kept Traditionally vs. Those Kept for Commercial Purposes. in Insects
MDPI., 11(266), 266.
https://doi.org/10.3390/insects11050266

Tarić E, Glavinić U, Vejnović B, Stanojković A, Aleksić N, Dimitrijević V, Stanimirović Z. Oxidative Stress, Endoparasite Prevalence and Social Immunity in Bee Colonies Kept Traditionally vs. Those Kept for Commercial Purposes. in Insects. 2020;11(266):266.
doi:10.3390/insects11050266 .

Tarić, Elmin, Glavinić, Uroš, Vejnović, Branislav, Stanojković, Aleksandar, Aleksić, Nevenka, Dimitrijević, Vladimir, Stanimirović, Zoran, "Oxidative Stress, Endoparasite Prevalence and Social Immunity in Bee Colonies Kept Traditionally vs. Those Kept for Commercial Purposes" in Insects, 11, no. 266 (2020):266,
https://doi.org/10.3390/insects11050266 . .

TY  - JOUR
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Zorić, Andrea
AU  - Krnjaić, Dejan
AU  - Radojičić, Marina
AU  - Stanojković, Aleksandar
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1453
AB  - Newcastle disease virus (NDV) is one of the most important viral pathogens of avian species and the causative agent of atypical fowl plague, a highly contagious and economically important disease characterized by high mortality rates and reduction of egg production. The HN and F proteins are the main targets for immune response to NDV. Vaccination of poultry with live and inactivated NDV vaccines is the most effective method of control and prevention of Newcastle disease, however due to their disadvantages, efforts are being invested into developing subunit vaccines. To this end, the NDV HN and/or F protein have been expressed using different viruses as vectors, but have also been expressed using transgenic plant systems, yeast and lactic acid bacteria in order to produce the NDV subunit vaccine. Many authors have investigated the possibility of preparation of vaccines from purified and biologically active NDV subunits with HN and F glycoproteins, purified from nucleocapsids, viral ribonucleic acid (RNA) and pyrogens. The above mentioned viral glycoproteins with preserved antigenic structure and biological activities can be used as subunit vaccinal antigens due to their immunogenic properties.
AB  - Virus Newcastle bolesti je jedan od najznačajnijih patogena u populaciji ptica i domaće živine koji izaziva atipičnu kugu živine, kontagiozno oboljenje koje prati visoka stopa morbiditeta i mortaliteta, što ima za posledicu i velike ekonomske gubitke u živinarstvu. Glikoproteinski HN i F antigeni virusa atipične kuge živine su najznačajniji prilikom razvoja imunološkog odgovora prijemčivih jedinki. Vakcinacija živine živim i inaktivisanim vakcinama protiv virusa Newcastle bolesti predstavlja najefikasniji metod kontrole i prevencije navedenog oboljenja, međutim klasične vakcine imaju izvesne nedostatke i iz tog razloga se sve više istraživanja se usmerava na razvoj subjediničnih vakcina. U cilju razvoja subjediničnih vakcina u današnje vreme se za ekspresiju HN i F proteina virusa Newcastle bolesti koriste različiti vektori kao što su virusi, transgene biljke, kvasci i mlečnokiselinske bakterije. Pored toga, mnogi autori su ispitivali mogućnosti pripremanja subjediničnih vakcina od prečišćenih i biološki aktivnih subjedinica, odnosno HN i F glikoproteina pomenutog virusa, oslobođenih od nukleokapsida sa virusnom ribonukleinskom kiselinom (RNK) i pirogena. Virusni glikoproteini sa očuvanom antigenskom strukturom i biološkim aktivnostima se zbog svojih imunogenih svojstava mogu koristiti kao subjedinični vakcinalni antigeni.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus
T1  - Pregled savremenih saznanja o razvoju subjediničnih i rekombinantnih vakcina protiv virusa Newcastle bolesti živine
VL  - 33
IS  - 1
SP  - 1
EP  - 11
DO  - 10.2298/BAH1701001M
ER  - 

@article{
author = "Milić, Nenad and Nišavić, Jakov and Zorić, Andrea and Krnjaić, Dejan and Radojičić, Marina and Stanojković, Aleksandar",
year = "2017",
abstract = "Newcastle disease virus (NDV) is one of the most important viral pathogens of avian species and the causative agent of atypical fowl plague, a highly contagious and economically important disease characterized by high mortality rates and reduction of egg production. The HN and F proteins are the main targets for immune response to NDV. Vaccination of poultry with live and inactivated NDV vaccines is the most effective method of control and prevention of Newcastle disease, however due to their disadvantages, efforts are being invested into developing subunit vaccines. To this end, the NDV HN and/or F protein have been expressed using different viruses as vectors, but have also been expressed using transgenic plant systems, yeast and lactic acid bacteria in order to produce the NDV subunit vaccine. Many authors have investigated the possibility of preparation of vaccines from purified and biologically active NDV subunits with HN and F glycoproteins, purified from nucleocapsids, viral ribonucleic acid (RNA) and pyrogens. The above mentioned viral glycoproteins with preserved antigenic structure and biological activities can be used as subunit vaccinal antigens due to their immunogenic properties., Virus Newcastle bolesti je jedan od najznačajnijih patogena u populaciji ptica i domaće živine koji izaziva atipičnu kugu živine, kontagiozno oboljenje koje prati visoka stopa morbiditeta i mortaliteta, što ima za posledicu i velike ekonomske gubitke u živinarstvu. Glikoproteinski HN i F antigeni virusa atipične kuge živine su najznačajniji prilikom razvoja imunološkog odgovora prijemčivih jedinki. Vakcinacija živine živim i inaktivisanim vakcinama protiv virusa Newcastle bolesti predstavlja najefikasniji metod kontrole i prevencije navedenog oboljenja, međutim klasične vakcine imaju izvesne nedostatke i iz tog razloga se sve više istraživanja se usmerava na razvoj subjediničnih vakcina. U cilju razvoja subjediničnih vakcina u današnje vreme se za ekspresiju HN i F proteina virusa Newcastle bolesti koriste različiti vektori kao što su virusi, transgene biljke, kvasci i mlečnokiselinske bakterije. Pored toga, mnogi autori su ispitivali mogućnosti pripremanja subjediničnih vakcina od prečišćenih i biološki aktivnih subjedinica, odnosno HN i F glikoproteina pomenutog virusa, oslobođenih od nukleokapsida sa virusnom ribonukleinskom kiselinom (RNK) i pirogena. Virusni glikoproteini sa očuvanom antigenskom strukturom i biološkim aktivnostima se zbog svojih imunogenih svojstava mogu koristiti kao subjedinični vakcinalni antigeni.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus, Pregled savremenih saznanja o razvoju subjediničnih i rekombinantnih vakcina protiv virusa Newcastle bolesti živine",
volume = "33",
number = "1",
pages = "1-11",
doi = "10.2298/BAH1701001M"
}

Milić, N., Nišavić, J., Zorić, A., Krnjaić, D., Radojičić, M.,& Stanojković, A.. (2017). Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 33(1), 1-11.
https://doi.org/10.2298/BAH1701001M

Milić N, Nišavić J, Zorić A, Krnjaić D, Radojičić M, Stanojković A. Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus. in Biotechnology in Animal Husbandry. 2017;33(1):1-11.
doi:10.2298/BAH1701001M .

Milić, Nenad, Nišavić, Jakov, Zorić, Andrea, Krnjaić, Dejan, Radojičić, Marina, Stanojković, Aleksandar, "Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus" in Biotechnology in Animal Husbandry, 33, no. 1 (2017):1-11,
https://doi.org/10.2298/BAH1701001M . .

TY  - JOUR
AU  - Nišavić, Jakov
AU  - Milić, Nenad
AU  - Zorić, Andrea
AU  - Bojkovski, Jovan
AU  - Stanojković, Aleksandar
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1320
AB  - Viral infections of swine cause significant economic losses in swine husbandry. They manifest in death of infected animals of different ages or in decreased productivity during the manufacturing process. Having that in mind, rapid and reliable diagnostics of viral infections is crucial in the prevention of disease transmission in herds of swine. Today, virological laboratories all over the world use different diagnostic methods such as isolation of virus in cell lines, ELISA, virus neutralization test, direct and indirect immunofluorescence and hemagglutination and hemagglutination inhibition tests. Virus isolation, virus neutralization test and some other standard virological methods are time consuming and rather expensive, therefore, molecular methods such as conventional PCR, RT - PCR, real-time PCR and direct sequencing methods are applied worldwide as fast and reliable. Their application is especially necessary for the detection of viruses which cannot be identified by using standard virological methods.
AB  - Virusne infekcije izazivaju značajne ekonomske gubitke u svinjarskoj proizvodnji. One se ispoljavaju kako kroz pojavu uginuća životinja, tako i kroz smanjenje produktivnosti. U cilju otkrivanja i sprečavanja širenja virusnih oboljenja svinja danas se u svetu primenjuju standardne i molekularne metode virusološke dijagnostike. Od standardnih metoda dijagnostike u upotrebi su metode izolacije virusa u kulturi ćelija, zatim ELISA, direktna i indirektna imunofluorescencija, kao i hemaglutinacija i inhibicija hemaglutinacije. Primena navedenih metoda podrazumeva duže vreme potrebno za dobijanje rezultata ispitivanja od najmanje 5 do 7 dana. Međutim, primena savremenih molekularnih metoda virusološke dijagnostike kao što su PCR, real-time PCR, odnosno metoda direktnog sekvenciranja, podrazumeva kraće vreme potrebno za dobijanje rezultata, odnosno omogućava preciznu dijagnostiku oboljenja u kraćem vremenskom periodu. Pored ovoga, značaj primene ovih metoda se ogleda i u otkrivanju virusa čije se prisustvo u uzorcima na drugi način, odnosno primenom standardnih metoda virusološke dijagnostike ne može detektovati.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - The application of PCR based methods in diagnostics of some viral infections of swine
T1  - Primena molekularnih metoda zasnovanih na lančanoj reakciji polimeraze u dijagnostici nekih infekcija svinja
VL  - 32
IS  - 4
SP  - 321
EP  - 329
DO  - 10.2298/bah1604321N
ER  - 

@article{
author = "Nišavić, Jakov and Milić, Nenad and Zorić, Andrea and Bojkovski, Jovan and Stanojković, Aleksandar",
year = "2016",
abstract = "Viral infections of swine cause significant economic losses in swine husbandry. They manifest in death of infected animals of different ages or in decreased productivity during the manufacturing process. Having that in mind, rapid and reliable diagnostics of viral infections is crucial in the prevention of disease transmission in herds of swine. Today, virological laboratories all over the world use different diagnostic methods such as isolation of virus in cell lines, ELISA, virus neutralization test, direct and indirect immunofluorescence and hemagglutination and hemagglutination inhibition tests. Virus isolation, virus neutralization test and some other standard virological methods are time consuming and rather expensive, therefore, molecular methods such as conventional PCR, RT - PCR, real-time PCR and direct sequencing methods are applied worldwide as fast and reliable. Their application is especially necessary for the detection of viruses which cannot be identified by using standard virological methods., Virusne infekcije izazivaju značajne ekonomske gubitke u svinjarskoj proizvodnji. One se ispoljavaju kako kroz pojavu uginuća životinja, tako i kroz smanjenje produktivnosti. U cilju otkrivanja i sprečavanja širenja virusnih oboljenja svinja danas se u svetu primenjuju standardne i molekularne metode virusološke dijagnostike. Od standardnih metoda dijagnostike u upotrebi su metode izolacije virusa u kulturi ćelija, zatim ELISA, direktna i indirektna imunofluorescencija, kao i hemaglutinacija i inhibicija hemaglutinacije. Primena navedenih metoda podrazumeva duže vreme potrebno za dobijanje rezultata ispitivanja od najmanje 5 do 7 dana. Međutim, primena savremenih molekularnih metoda virusološke dijagnostike kao što su PCR, real-time PCR, odnosno metoda direktnog sekvenciranja, podrazumeva kraće vreme potrebno za dobijanje rezultata, odnosno omogućava preciznu dijagnostiku oboljenja u kraćem vremenskom periodu. Pored ovoga, značaj primene ovih metoda se ogleda i u otkrivanju virusa čije se prisustvo u uzorcima na drugi način, odnosno primenom standardnih metoda virusološke dijagnostike ne može detektovati.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "The application of PCR based methods in diagnostics of some viral infections of swine, Primena molekularnih metoda zasnovanih na lančanoj reakciji polimeraze u dijagnostici nekih infekcija svinja",
volume = "32",
number = "4",
pages = "321-329",
doi = "10.2298/bah1604321N"
}

Nišavić, J., Milić, N., Zorić, A., Bojkovski, J.,& Stanojković, A.. (2016). The application of PCR based methods in diagnostics of some viral infections of swine. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 32(4), 321-329.
https://doi.org/10.2298/bah1604321N

Nišavić J, Milić N, Zorić A, Bojkovski J, Stanojković A. The application of PCR based methods in diagnostics of some viral infections of swine. in Biotechnology in Animal Husbandry. 2016;32(4):321-329.
doi:10.2298/bah1604321N .

Nišavić, Jakov, Milić, Nenad, Zorić, Andrea, Bojkovski, Jovan, Stanojković, Aleksandar, "The application of PCR based methods in diagnostics of some viral infections of swine" in Biotechnology in Animal Husbandry, 32, no. 4 (2016):321-329,
https://doi.org/10.2298/bah1604321N . .

TY  - JOUR
AU  - Stanojković, Aleksandar
AU  - Ašanin, Ružica
AU  - Mišić, Dušan
AU  - Ašanin, Jelena
AU  - Stanojkovic-Sebić, Aleksandra
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/868
AB  - Streptococcus suis is a normal inhabitant of the respiratory system of pigs. Clinically healthy pigs are a major reservoir of the pathogen, but, as well, the most important link in the epidemiology of infection in humans. Hence, the aim of this study was to establish the presence of S. suis on some pig farms in Serbia and to determine their serotype affiliation. For this research, we tested 226 samples: nasopharyngeal tonsil swabs, nasal cavities swabs, parts of or all nasopharyngeal tonsils, swabs from dead animals, swabs from pig carcasses, and swabs from the butchers knives. Identification of S. suis strains was performed with antisera specific for capsular antigens. S. suis serotype 2 was established in 67.7% cases, and serotypes 7, 9 and 1 were isolated in a much smaller percentage (17.6, 8.8 and 5.9%, respectively). Serotype 2 is the only serotype isolated from all the collected samples. Serotype 9 was isolated from healthy pig tonsil swab and from 2 nasal swabs, and was not found in the samples taken from diseased pigs. The serotype 1 was isolated only from nasopharyngeal tonsil swabs of piglets, while serotype 7 was isolated from nasopharyngeal tonsil swabs, nasopharyngeal tonsil sections, but also from nasal swabs of healthy pigs. It can be concluded that the infections caused by different serotypes of S. suis are present in the herd of pigs in Serbia; hence, it is very important to identify what serotypes of S. suis are involved in pathogenesis of infections in pigs.
PB  - Parlar Scientific Publications (P S P), Freising
T2  - Fresenius Environmental Bulletin
T1  - The presence and serological types of streptococcus suis strains isolated from pigs originating from some farms in Serbia
VL  - 21
IS  - 11C
SP  - 3558
EP  - 3561
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_868
ER  - 

@article{
author = "Stanojković, Aleksandar and Ašanin, Ružica and Mišić, Dušan and Ašanin, Jelena and Stanojkovic-Sebić, Aleksandra",
year = "2012",
abstract = "Streptococcus suis is a normal inhabitant of the respiratory system of pigs. Clinically healthy pigs are a major reservoir of the pathogen, but, as well, the most important link in the epidemiology of infection in humans. Hence, the aim of this study was to establish the presence of S. suis on some pig farms in Serbia and to determine their serotype affiliation. For this research, we tested 226 samples: nasopharyngeal tonsil swabs, nasal cavities swabs, parts of or all nasopharyngeal tonsils, swabs from dead animals, swabs from pig carcasses, and swabs from the butchers knives. Identification of S. suis strains was performed with antisera specific for capsular antigens. S. suis serotype 2 was established in 67.7% cases, and serotypes 7, 9 and 1 were isolated in a much smaller percentage (17.6, 8.8 and 5.9%, respectively). Serotype 2 is the only serotype isolated from all the collected samples. Serotype 9 was isolated from healthy pig tonsil swab and from 2 nasal swabs, and was not found in the samples taken from diseased pigs. The serotype 1 was isolated only from nasopharyngeal tonsil swabs of piglets, while serotype 7 was isolated from nasopharyngeal tonsil swabs, nasopharyngeal tonsil sections, but also from nasal swabs of healthy pigs. It can be concluded that the infections caused by different serotypes of S. suis are present in the herd of pigs in Serbia; hence, it is very important to identify what serotypes of S. suis are involved in pathogenesis of infections in pigs.",
publisher = "Parlar Scientific Publications (P S P), Freising",
journal = "Fresenius Environmental Bulletin",
title = "The presence and serological types of streptococcus suis strains isolated from pigs originating from some farms in Serbia",
volume = "21",
number = "11C",
pages = "3558-3561",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_868"
}

Stanojković, A., Ašanin, R., Mišić, D., Ašanin, J.,& Stanojkovic-Sebić, A.. (2012). The presence and serological types of streptococcus suis strains isolated from pigs originating from some farms in Serbia. in Fresenius Environmental Bulletin
Parlar Scientific Publications (P S P), Freising., 21(11C), 3558-3561.
https://hdl.handle.net/21.15107/rcub_veterinar_868

Stanojković A, Ašanin R, Mišić D, Ašanin J, Stanojkovic-Sebić A. The presence and serological types of streptococcus suis strains isolated from pigs originating from some farms in Serbia. in Fresenius Environmental Bulletin. 2012;21(11C):3558-3561.
https://hdl.handle.net/21.15107/rcub_veterinar_868 .

Stanojković, Aleksandar, Ašanin, Ružica, Mišić, Dušan, Ašanin, Jelena, Stanojkovic-Sebić, Aleksandra, "The presence and serological types of streptococcus suis strains isolated from pigs originating from some farms in Serbia" in Fresenius Environmental Bulletin, 21, no. 11C (2012):3558-3561,
https://hdl.handle.net/21.15107/rcub_veterinar_868 .