TY  - JOUR
AU  - Gray, Alison
AU  - Adjlane, Noureddine
AU  - Arab, Alireza
AU  - Ballis, Alexis
AU  - Brusbardis, Valters
AU  - Bugeja Douglas, Adrian
AU  - Cadahía, Luis
AU  - Charrière, Jean-Daniel
AU  - Chlebo, Robert
AU  - Coffey, Mary F.
AU  - Cornelissen, Bram
AU  - Costa, Cristina Amaro da
AU  - Danneels, Ellen
AU  - Danihlík, Jiří
AU  - Dobrescu, Constantin
AU  - Evans, Garth
AU  - Fedoriak, Mariia
AU  - Forsythe, Ivan
AU  - Gregorc, Aleš
AU  - Ilieva Arakelyan, Iliyana
AU  - Johannesen, Jes
AU  - Kauko, Lassi
AU  - Kristiansen, Preben
AU  - Martikkala, Maritta
AU  - Martín-Hernández, Raquel
AU  - Mazur, Ewa
AU  - Medina-Flores, Carlos Aurelio
AU  - Mutinelli, Franco
AU  - Omar, Eslam M.
AU  - Patalano, Solenn
AU  - Raudmets, Aivar
AU  - San Martin, Gilles
AU  - Soroker, Victoria
AU  - Stahlmann-Brown, Philip
AU  - Stevanović, Jevrosima
AU  - Uzunov, Aleksandar
AU  - Vejsnaes, Flemming
AU  - Williams, Anthony
AU  - Brodschneider, Robert
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2504
AB  - This article presents managed honey bee colony loss rates over winter 2019/20 resulting from using the standardised COLOSS questionnaire in 37 countries. Six countries were from outside Europe, including, for the first time in this series of articles, New Zealand. The 30,491 beekeepers outside New Zealand reported 4.5% of colonies with unsolvable queen problems, 11.1% of colonies dead after winter and 2.6% lost through natural disaster. This gave an overall colony winter loss rate of 18.1%, higher than in the previous year. The winter loss rates varied greatly between countries, from 7.4% to 36.5%. 3216 beekeepers from New Zealand managing 297,345 colonies reported 10.5% losses for their 2019 winter (six months earlier than for other, Northern Hemisphere, countries). We modelled the risk of loss as a dead/empty colony or from unresolvable queen problems, for all countries except New Zealand. Overall, larger beekeeping operations with more than 50 colonies experienced significantly lower losses (p < 0.001). Migration was also highly significant (p < 0.001), with lower loss rates for operations migrating their colonies in the previous season. A higher proportion of new queens reduced the risk of colony winter loss (p < 0.001), suggesting that more queen replacement is better. All three factors, operation size, migration and proportion of young queens, were also included in a multivariable main effects quasi-binomial GLM and all three remained highly significant (p < 0.001). Detailed results for each country and overall are given in a table, and a map shows relative risks of winter loss at the regional level.
T2  - Journal of Apicultural Research
T1  - Honey bee colony loss rates in 37 countries using the COLOSS survey for winter 2019–2020: the combined effects of operation size, migration and queen replacement
VL  - 62
IS  - 2
SP  - 204
EP  - 210
DO  - 10.1080/00218839.2022.2113329
ER  - 

@article{
author = "Gray, Alison and Adjlane, Noureddine and Arab, Alireza and Ballis, Alexis and Brusbardis, Valters and Bugeja Douglas, Adrian and Cadahía, Luis and Charrière, Jean-Daniel and Chlebo, Robert and Coffey, Mary F. and Cornelissen, Bram and Costa, Cristina Amaro da and Danneels, Ellen and Danihlík, Jiří and Dobrescu, Constantin and Evans, Garth and Fedoriak, Mariia and Forsythe, Ivan and Gregorc, Aleš and Ilieva Arakelyan, Iliyana and Johannesen, Jes and Kauko, Lassi and Kristiansen, Preben and Martikkala, Maritta and Martín-Hernández, Raquel and Mazur, Ewa and Medina-Flores, Carlos Aurelio and Mutinelli, Franco and Omar, Eslam M. and Patalano, Solenn and Raudmets, Aivar and San Martin, Gilles and Soroker, Victoria and Stahlmann-Brown, Philip and Stevanović, Jevrosima and Uzunov, Aleksandar and Vejsnaes, Flemming and Williams, Anthony and Brodschneider, Robert",
year = "2023",
abstract = "This article presents managed honey bee colony loss rates over winter 2019/20 resulting from using the standardised COLOSS questionnaire in 37 countries. Six countries were from outside Europe, including, for the first time in this series of articles, New Zealand. The 30,491 beekeepers outside New Zealand reported 4.5% of colonies with unsolvable queen problems, 11.1% of colonies dead after winter and 2.6% lost through natural disaster. This gave an overall colony winter loss rate of 18.1%, higher than in the previous year. The winter loss rates varied greatly between countries, from 7.4% to 36.5%. 3216 beekeepers from New Zealand managing 297,345 colonies reported 10.5% losses for their 2019 winter (six months earlier than for other, Northern Hemisphere, countries). We modelled the risk of loss as a dead/empty colony or from unresolvable queen problems, for all countries except New Zealand. Overall, larger beekeeping operations with more than 50 colonies experienced significantly lower losses (p < 0.001). Migration was also highly significant (p < 0.001), with lower loss rates for operations migrating their colonies in the previous season. A higher proportion of new queens reduced the risk of colony winter loss (p < 0.001), suggesting that more queen replacement is better. All three factors, operation size, migration and proportion of young queens, were also included in a multivariable main effects quasi-binomial GLM and all three remained highly significant (p < 0.001). Detailed results for each country and overall are given in a table, and a map shows relative risks of winter loss at the regional level.",
journal = "Journal of Apicultural Research",
title = "Honey bee colony loss rates in 37 countries using the COLOSS survey for winter 2019–2020: the combined effects of operation size, migration and queen replacement",
volume = "62",
number = "2",
pages = "204-210",
doi = "10.1080/00218839.2022.2113329"
}

Gray, A., Adjlane, N., Arab, A., Ballis, A., Brusbardis, V., Bugeja Douglas, A., Cadahía, L., Charrière, J., Chlebo, R., Coffey, M. F., Cornelissen, B., Costa, C. A. d., Danneels, E., Danihlík, J., Dobrescu, C., Evans, G., Fedoriak, M., Forsythe, I., Gregorc, A., Ilieva Arakelyan, I., Johannesen, J., Kauko, L., Kristiansen, P., Martikkala, M., Martín-Hernández, R., Mazur, E., Medina-Flores, C. A., Mutinelli, F., Omar, E. M., Patalano, S., Raudmets, A., San Martin, G., Soroker, V., Stahlmann-Brown, P., Stevanović, J., Uzunov, A., Vejsnaes, F., Williams, A.,& Brodschneider, R.. (2023). Honey bee colony loss rates in 37 countries using the COLOSS survey for winter 2019–2020: the combined effects of operation size, migration and queen replacement. in Journal of Apicultural Research, 62(2), 204-210.
https://doi.org/10.1080/00218839.2022.2113329

Gray A, Adjlane N, Arab A, Ballis A, Brusbardis V, Bugeja Douglas A, Cadahía L, Charrière J, Chlebo R, Coffey MF, Cornelissen B, Costa CAD, Danneels E, Danihlík J, Dobrescu C, Evans G, Fedoriak M, Forsythe I, Gregorc A, Ilieva Arakelyan I, Johannesen J, Kauko L, Kristiansen P, Martikkala M, Martín-Hernández R, Mazur E, Medina-Flores CA, Mutinelli F, Omar EM, Patalano S, Raudmets A, San Martin G, Soroker V, Stahlmann-Brown P, Stevanović J, Uzunov A, Vejsnaes F, Williams A, Brodschneider R. Honey bee colony loss rates in 37 countries using the COLOSS survey for winter 2019–2020: the combined effects of operation size, migration and queen replacement. in Journal of Apicultural Research. 2023;62(2):204-210.
doi:10.1080/00218839.2022.2113329 .

Gray, Alison, Adjlane, Noureddine, Arab, Alireza, Ballis, Alexis, Brusbardis, Valters, Bugeja Douglas, Adrian, Cadahía, Luis, Charrière, Jean-Daniel, Chlebo, Robert, Coffey, Mary F., Cornelissen, Bram, Costa, Cristina Amaro da, Danneels, Ellen, Danihlík, Jiří, Dobrescu, Constantin, Evans, Garth, Fedoriak, Mariia, Forsythe, Ivan, Gregorc, Aleš, Ilieva Arakelyan, Iliyana, Johannesen, Jes, Kauko, Lassi, Kristiansen, Preben, Martikkala, Maritta, Martín-Hernández, Raquel, Mazur, Ewa, Medina-Flores, Carlos Aurelio, Mutinelli, Franco, Omar, Eslam M., Patalano, Solenn, Raudmets, Aivar, San Martin, Gilles, Soroker, Victoria, Stahlmann-Brown, Philip, Stevanović, Jevrosima, Uzunov, Aleksandar, Vejsnaes, Flemming, Williams, Anthony, Brodschneider, Robert, "Honey bee colony loss rates in 37 countries using the COLOSS survey for winter 2019–2020: the combined effects of operation size, migration and queen replacement" in Journal of Apicultural Research, 62, no. 2 (2023):204-210,
https://doi.org/10.1080/00218839.2022.2113329 . .

TY  - CONF
AU  - Glavinić, Uroš
AU  - Ristanić, Marko
AU  - Jovanović, Nemanja M.
AU  - Rajković, Milan
AU  - Niketić, Mia
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2419
AB  - donosne pčele (Apis mellifera). Izaziva bolest nozemozu (nozematozu) i u zavisnosti od stepena infekcije i jačine pčelinjih društava,
ostavlja značajne posledice na zdravlje, reproduktivne i produktivne
sposobnosti pčela.
Antibiotik fumagilin je godinama bio jedini lek dostupan za kontrolu ovog patogena. Njegova primena dovedena je u pitanje zbog
nedostataka koji prate njegovu primenu (efikasnost, mortalitet pčela i pojava rezidua u pčelinjim proizvodima). Širom sveta se sprovode istraživanja sa ciljem pronalaska alternative za kontrolu nozeme.
U ovom radu prikazani su naši rezultati ispitivanja dijetetskih suplemenata na bazi prirodnih/biljnih ekstrakata (Medenko forte i ekstrakti gljiva Agaricus blazei i A. bisporus), timola i vitaminsko-mineralnog
aditiva (BEEWELL AminoPlus). Kod pčela tretiranih svim nabrojanim
suplementima i fumagilinom kao kontrolnim tretmanom, praćen je broj
spora N. ceranae, nivoi ekspresije gena značajnih za imunitet pčela
kao i parametri oksidativnog stresa. Svi preparati primenjivani su preventivno (tri dana pre infekcije), u trenutku inficiranja, kao i tri dana nakon infekcije sa N. ceranae, u cilju određivanja najboljeg momenta za
primenu. Dokazani su negativni efekti fumagilina kada je preventivno
primenjivan kod neinficiranih pčela. Kod inficiranih pčela većina testiranih suplemenata dovela je do smanjenja broja spora N. ceranae, a
pokazali su i zaštitni efekat u smislu sprečavanja imunosupresije i oksidativnog stresa.
AB  - The microsporidian Nosema ceranae is an obligate intestinal parasite of the honey
bee (Apis mellifera). It causes nosemosis and, depending on the infection level and
the strength of the infected bee colonies, causes significant health disorders, including
malfunction of reproductive and productive capabilities of bees. The antibiotic fumagillin
was the only drug available for Nosema control for many years. However, use of fumagillin
has been questioned due to the drawbacks related to its efficacy, bee mortality and the
presence of residues in bee products. Studies aimed to find alternatives for Nosema control
are being conducted worldwide. In this paper, our results related to diet supplements based
on natural/plant extracts (Medenko forte and extracts of Agaricus blazei and A. bisporus),
thymol and vitamin-mineral additive (BEEWELL AminoPlus) are presented. Bees were
treated with these supplements and fumagillin (control treatment) and gene expression
levels (important for bee immunity), as well as oxidative stress parameters were assessed.
In order to determine adequate time for application, all supplements were applied
preventively (three days before infection), at the time of infection, as well as three days after
N. ceranae infection. In uninfected bees, fumagillin demonstrated adverse effects when
applied prophylactically. In Nosema-infected bees, tested supplements mainly contributed
to the decrease of N. ceranae spore levels, but also exhibited a protective effect based on
level of immune-related gene expression and parameters of oxidative stress.
PB  - Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - Zbornik radova - XLIII seminar inovacija znanja veterinara
T1  - Novi trendovi u kontroli Nosema ceranae
T1  - Advances in Nosema ceranae control
SP  - 79
EP  - 91
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2419
ER  - 

@conference{
author = "Glavinić, Uroš and Ristanić, Marko and Jovanović, Nemanja M. and Rajković, Milan and Niketić, Mia and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2022",
abstract = "donosne pčele (Apis mellifera). Izaziva bolest nozemozu (nozematozu) i u zavisnosti od stepena infekcije i jačine pčelinjih društava,
ostavlja značajne posledice na zdravlje, reproduktivne i produktivne
sposobnosti pčela.
Antibiotik fumagilin je godinama bio jedini lek dostupan za kontrolu ovog patogena. Njegova primena dovedena je u pitanje zbog
nedostataka koji prate njegovu primenu (efikasnost, mortalitet pčela i pojava rezidua u pčelinjim proizvodima). Širom sveta se sprovode istraživanja sa ciljem pronalaska alternative za kontrolu nozeme.
U ovom radu prikazani su naši rezultati ispitivanja dijetetskih suplemenata na bazi prirodnih/biljnih ekstrakata (Medenko forte i ekstrakti gljiva Agaricus blazei i A. bisporus), timola i vitaminsko-mineralnog
aditiva (BEEWELL AminoPlus). Kod pčela tretiranih svim nabrojanim
suplementima i fumagilinom kao kontrolnim tretmanom, praćen je broj
spora N. ceranae, nivoi ekspresije gena značajnih za imunitet pčela
kao i parametri oksidativnog stresa. Svi preparati primenjivani su preventivno (tri dana pre infekcije), u trenutku inficiranja, kao i tri dana nakon infekcije sa N. ceranae, u cilju određivanja najboljeg momenta za
primenu. Dokazani su negativni efekti fumagilina kada je preventivno
primenjivan kod neinficiranih pčela. Kod inficiranih pčela većina testiranih suplemenata dovela je do smanjenja broja spora N. ceranae, a
pokazali su i zaštitni efekat u smislu sprečavanja imunosupresije i oksidativnog stresa., The microsporidian Nosema ceranae is an obligate intestinal parasite of the honey
bee (Apis mellifera). It causes nosemosis and, depending on the infection level and
the strength of the infected bee colonies, causes significant health disorders, including
malfunction of reproductive and productive capabilities of bees. The antibiotic fumagillin
was the only drug available for Nosema control for many years. However, use of fumagillin
has been questioned due to the drawbacks related to its efficacy, bee mortality and the
presence of residues in bee products. Studies aimed to find alternatives for Nosema control
are being conducted worldwide. In this paper, our results related to diet supplements based
on natural/plant extracts (Medenko forte and extracts of Agaricus blazei and A. bisporus),
thymol and vitamin-mineral additive (BEEWELL AminoPlus) are presented. Bees were
treated with these supplements and fumagillin (control treatment) and gene expression
levels (important for bee immunity), as well as oxidative stress parameters were assessed.
In order to determine adequate time for application, all supplements were applied
preventively (three days before infection), at the time of infection, as well as three days after
N. ceranae infection. In uninfected bees, fumagillin demonstrated adverse effects when
applied prophylactically. In Nosema-infected bees, tested supplements mainly contributed
to the decrease of N. ceranae spore levels, but also exhibited a protective effect based on
level of immune-related gene expression and parameters of oxidative stress.",
publisher = "Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "Zbornik radova - XLIII seminar inovacija znanja veterinara",
title = "Novi trendovi u kontroli Nosema ceranae, Advances in Nosema ceranae control",
pages = "79-91",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2419"
}

Glavinić, U., Ristanić, M., Jovanović, N. M., Rajković, M., Niketić, M., Stevanović, J.,& Stanimirović, Z.. (2022). Novi trendovi u kontroli Nosema ceranae. in Zbornik radova - XLIII seminar inovacija znanja veterinara
Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 79-91.
https://hdl.handle.net/21.15107/rcub_veterinar_2419

Glavinić U, Ristanić M, Jovanović NM, Rajković M, Niketić M, Stevanović J, Stanimirović Z. Novi trendovi u kontroli Nosema ceranae. in Zbornik radova - XLIII seminar inovacija znanja veterinara. 2022;:79-91.
https://hdl.handle.net/21.15107/rcub_veterinar_2419 .

Glavinić, Uroš, Ristanić, Marko, Jovanović, Nemanja M., Rajković, Milan, Niketić, Mia, Stevanović, Jevrosima, Stanimirović, Zoran, "Novi trendovi u kontroli Nosema ceranae" in Zbornik radova - XLIII seminar inovacija znanja veterinara (2022):79-91,
https://hdl.handle.net/21.15107/rcub_veterinar_2419 .

TY  - THES
AU  - Tarić, Elmin
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3360
AB  - Doktroska disertacija
AB  - Štetoĉine i patogeni predstavljaju najĉešće uzroke gubitaka pĉelinjih društva.
Ameriĉka truleţ (AFB) je bolest pĉelinjeg legla, uzrokovana bakterijom Paenibacillus.
larvae, White (1906), moţe se smatrati glavnom pretnjom po zdravlje pĉela, s obzirom
da je reĉ o panzootskoj bolesti koja se vrlo brzo širi, ne samo iz košnice u košnicu, sa
pĉelinjaka na pĉelinjak iz regije u regiju, već i iz drţave u drţavu i šire. Evropska truleţ
je bakterijska bolest pĉelinjeg legla uzrokovana sa više bakterisjkih vrsta gde dominira
Melissococcus plutonius. Ova bolest je jako raširena i predstavlja veliki problem u
pĉelarstvu smanjujući proizvodne rezulte pĉelinjih zajednica. Kreĉno leglo (CHB) je
infekcija izazvana gljivicom Ascosphaera apis izazivajući gubitke komercijalno gajenih
pĉelinjih zajednica, naroĉito u kombinaciji sa vrstama mikrosporidija roda Nosema i
virusom mešinastog legla. Nozemoza je najĉešća bolest meĊu odraslim pĉelama
uzrokovana mirkosporidijama Nosema apis i N. ceranae i ĉesto dovodi do ekonomskih
gubitaka u pĉelarstvu. Virusne infekcije pĉela, koje su već odavno dostigle razmere
panzootije, ugroţavaju zdravlje pĉela i predstavljaju konaĉne egzekutore pĉelinjih
zajdnica. Pĉelinji krpelj Varroa destructor je glavni vektor skoro svih, a naroĉito
virusnih infekcija pĉela, koje su postale ozbiljan problem ne samo za komercijalno
gajene, već i za pĉelinje zajednice u divljini, zahvaljujući upravo krpelju V. destructor
kao fiziĉkom i biološkom vektoru Na osnovu svega navedenog jasno je da je
zdravstveno stanje društava u komercijalnom pĉelarstvu izloţeno velikim rizicima, a
njihovo ispitivanje i tretman predstavljaju veliki izazov kako za istraţivaĉe tako i za
pĉelare.
Bolesti odraslih pĉela i pĉelinjeg legla u komercijalnom pĉelarstvu najĉešće su
posledica energetskog stresa, nastalog zbog neadekvatne prihrane pĉelinjih zajednica,
prvenstveno prevelikom upotrebom sećernog sirupa. U osnovi energetskog stresa je
oksidativni stres pĉelinjih zajednica koji se moţe definisati kao disbalans izmeĊu
proizvodnje reaktivnih oblika kiseonika i antioksidativne odbrane. Reaktivni oblici
kiseonika (ROS) negativno utiĉu na ćelijske funkcije i stvaraju se tokom oksidoredukcionih metaboliĉkih procesa u ćeliji. Oni su ukljuĉeni u regulaciju razliĉitih
mehanizama, intercelularne signalizacije, a imaju i baktericidno dejstvo.
Medonosne pĉele, kao i ostale ţivotinje, razvile su niz enzimskih mehanizama
kojima se odupiru oksidativnom stresu, uklanjajući slobodne radikale. U ovim
procesima vaţnu ulogu umaju: superoksid dismutaza (SOD), enzim prisutan u citosolu i
mitohondrijama; katalaza (CAT) prisutna u peroksizomima glutation S-transferaza
(GST), glutation S transferaza (GST), peroksidaza i tireodoksin/tireoreduktazni sistem.
Imunitet pĉela, kao i imunitet u opšte, obuhvata kompleksan sistem koji ima cilj da
obezbedi oĉuvanje zdravlja kao i opstanak organizma na osnovu mnogih mehanizama
odbrane od patogena i drugih štetnih noksi (fiziĉkih i hemijskih). Medonosna pĉela svoj
socijalni imunitet ostvaruje uz pomoć bihejviorlanih mehanizama koji pomaţu zajdnici
u odbrani od patogena. Ulaganje u individualni imunitet ima visoku materijalnoenergetsku cenu za društvo, pa su udruţivanjem pĉela (razvoj socijlanog ustrojstva)
uspostavljeni zajedniĉki socijalni mehanizmi odbrane koji omogućavaju pĉelinjem
društvu efikasniju i ekonomiĉniju borbu protiv agenasa spoljašnje sredine. Za socijalni
imunitet pĉela od znaĉaja je enzim glukoza oksidazi (GOX). Ovaj enzim je produkt
pĉelinjih egzokrinih ţlezda, a uloga mu je u „konzervaciji― pĉelinjuh proizvoda, meda i
perge, tako spreĉavajući njihovo kvarenje. GOX uĉestvuje u procesu katalize -D
glukoze do glukonske kiseline i vodonik-peroksida. Vodonik-peroksid ima antiseptiĉka
svojstva, tako doprinoseći dodatnoj zaštiti i socijalnom imunitetu pĉela.
Cilj istraţivanja je bio da se utvrdi: da li izmeĊu tradicionalno i savremeno
gajenih pĉelinjih društava postoje razlike u pogledu prisustva patogena i pojave bolesti
pĉelinjeg legla, uz analizu bioloških (ekspresija GOX gena i faktora oksidativnog
stresa) i antropogenih faktora (menadţment u pĉelarstvu) na pojavu praćenih bolesti
pĉelinjeg legla i odraslih pĉela.
Terenski deo istraţivanja je obavljen na prostoru Pešterske visoravni (opština
Sjenica, Srbija) na 144 asimptomatske pĉelinje zajednice. Uzorci odraslih pĉela i
pĉelinjeg legla su uzeti iz savremenih (DB) košnica i tradicionalnih košnica „trmki―,
kako bi se utvrdila zastupljenost patogena legla (Paenibacilus larvae, Melissococus
plutonius, Ascosphera apis, virus mešinastog legla, tripanozoma Lotmaria passim i
Crithidia mellificae) i odraslih pĉela (virus akutne paralize pĉela, virus hroniĉne
paralize pĉela, virus deformisanih krila pĉela, tripanozoma Lotmaria passim i Crithidia
mellificae,mikrosporidija Nosema apis i Nosema ceranae ) kao i za utvrĊivanje nivoa
oksidativnog stresa analizom aktivnosti enzima: SOD, CAT, GST i koncentracije
malondialdehida (MDA). TakoĊe, iz istih košnica uzeti su uzorci za procenu ekspresije
GOX gena kao parametra socijlanog imuniteta.
Detekcija patogena je obavljena izolacijom bakterija kao i upotrebom PCR i
real-time PCR, u skladu sa standardima OIE. Rezultati istraţivanja su pokazali da je
meĊu komercijalno gajenim društvima P. larvae bio zastupljen u 16,67% uzoraka, A.
apis bio prisutan u 15,83% uzorka, dok je SBV detektovan u 96,67% uzoraka. MeĊutim
u leglu pĉela gajenih u tradicionalnim trmka košnicama naĊen je samo SBV u 33,33%
uzoraka. Dalja istraţivanja su utvrdila da su kod odraslih pĉela u komercijalno gajnim
društvima bili signifikantno (p<0,001) više zastupljeni virusi: ABPV, CBPV i DWV
(83,33%, 100,00%, 100,00%, istim redom) u odnosu na društva iz trmki gde je procenat
ovih virusa iznosio 33,33% za svaki virus. TakoĊe, sva komercijalno gajena društva bila
su inficirana makar jednim od praćenih patogena, za razliku od tradicionalno gajenih
pĉela u trmkama meĊu kojima je 66,66% bilo bez patogena.
Rezultati su otkrili znaĉajne razlike u aktivnosti CAT, GST i SOD (p<0,01) i
koncentracije MDA (p<0,002) izmeĊu komercijalnih i tradicionalnih društava što vodi
zakljuĉku da u društvima gajenim u trmkama postoji manji oksidativni stres što je
rezultiralo i manjom zastupljenošću svih praćenih patogena. U uzorcima komercijalno
gajenih pĉela zastupljenost parazita L. passim i N. ceranae bila je znaĉajno veća
(p<0,05; p<0,01, respektivno) u odnosu na uzorke iz tradicionalnih košnica, dok C.
mellificae i N. apis nisu detektovane ni u jednom uzorku. Treba istaći, da je prvi put
detektovana tripanozoma L. passim u leglu iz obe grupe ispitivanih košnica,
komercijalnih i tradicionalnih, pri ĉemu je njena zastupljenost u leglu znaĉajno manja
(p<0,01) nego u odraslim pĉelama u komercijalnim društvima, dok se kod tradicionalno
gajenih pĉela zastupljenost ove tripanozome izmeĊu odraslih pĉela i legla nije znaĉajno
razlikovala. Kod komercijalno gajenih pĉela utvrĊen je znaĉajno veći nivo iRNK za gen
GOX (p<0,01) u odnosu na tradicionalno gajena društva, što je verovatno posledica
pojaĉane potrebe prvopomenutih da ojaĉaju socijalni imunitet.
Komercijalna društva su u odnosu na tradicionalno gajena bila pod većim
oksidativnim stresom, a imala su i veću opterećenost pĉelinjim patogenima, kao i veći
nivo transkirpcije GOX gena, što je verovatno posledica ĉestih pĉelarskih manipulacija i
uznemiravanja komercijalnim društvima, kao i njihovo forsirano ekonomsko
iskorišćavanje. Sve to vodi zakljuĉku da populacija pĉela koja se uzgaja na
tradicionalan ima veći kapacitet samoodrţanja i otpornija na pĉelinje patogene,
energetski (i oksidativni) stres, a da antropogeni faktori, odnosno pĉelarski postupci,
imaju negativan uticaj na zdravlje komercijalno gajenih pĉela.
AB  - Pests and pathogens are the most common causes of honey bee colony losses. American
foulbrood (AFB) is a disease of honey bee brood, caused by the bacterium
Paenibacillus larvae, White (1906), can be considered a major threat to bee health, as it
is a panzootic disease that spreads very quickly, not only from hive to hive, from apiary
to apiary, from region to region, but also from the state to the state and beyond.
European foulbrood is a bacterial disease of honey bee brood caused by several
bacterial species dominated by Melissococcus plutonius. This disease is widespread and
poses a major problem in beekeeping by reducing the production results of honey bee
colonies. Chalkbrood disease (CHB) is an infection caused by the fungus Ascosphaera
apis causing losses of commercially reared bee colonies, especially in combination with
microsporidia species of the genus Nosema sp. and a Sacbrood virus (SBV). Nosemosis
is the most common disease among adult bees caused by Nosema apis and N. ceranae,
and often leads to economic losses in beekeeping. Viral infections of bees, which have
long since reached panzootic proportions, endanger the health of bees and present
ultimate executors of bee colonies. Varroa destructor mite is the main vector of almost
all, especially viral infections, which have become a serious problem not only for
commercially reared, but also for honey bees in the wild, thanks to the mite V.
destructor as a physical and biological vector. It is clear that the health status of
colonies in commercial beekeeping is exposed to great risks, and their examination and
treatment represent a great challenge for both researchers and beekeepers.
Diseases of adult bees and bee brood in commercial beekeeping are most often a
consequence of energetic stress, caused by inadequate feeding of bee colonies, primarily
by excessive use of sugar syrup. The basis of energetic stress is oxidative stress of bee
colonies, which can be defined as an imbalance between oxidants and antioxidants in
favor of the former. Reactive oxygen species (ROS) negatively affect cellular functions
and are generated during oxido-reduction metabolic processes in the cell. They are
involved in the regulation of various mechanisms, intercellular signaling, and exert
bactericidal activity.
Honey bees, like other animals, have developed a number of enzymatic mechanisms
that resist oxidative stress, removing free radicals. An important role in these processes
is played by: superoxide dismutase (SOD), catalase (CAT) in peroxisomes, ascorbate
peroxidase, glutathione S transferase (GST) peroxidase, and the thyrodoxin /
thyroreductase system. Immunity of bees, as well as immunity in general, includes a
complex system that aims to ensure the preservation of health and survival of the
organism based on many defense mechanisms against pathogens and other harmful
noxa (physical and chemical). The honey bee achieves its social immunity with the help
of behavioral mechanisms that help the community defend itself against pathogens.
Investing in individual immunity has a high material and energy price for society, so the
association of bees (development of social structure) established joint social defense
mechanisms that enable bee society to fight more efficiently and economically against
environmental agents. The enzyme glucose oxidase (GOX) is important for the social
immunity of bees. This enzyme is a product of bee exocrine glands, and its role is in the
"conservation" of bee products, honey and bee bread, thus preventing their spoilage.
GOX participates in the process of catalysis of -D glucose to gluconic acid and
hydrogen peroxide. Hydrogen peroxide has antiseptic properties, thus contributing to
additional protection and social immunity of bees.
The aim of the research was to determine: whether there are differences between
traditional and modern bee colonies in terms of the presence of pathogens and the
occurrence of bee brood diseases, with the analysis of biological (expression level of the
GOX gene and oxidative stress factors) and anthropogenic factors (beekeeping
management) on monitored bee brood and adult bee diseases. The field part of the
research was performed in the area of the Peshterska plateau (Sjenica municipality,
Serbia) on 144 asymptomatic bee communities. Samples of adult bees and bee brood
were taken from modern (DB) hives and traditional "trmka" hives to determine the
presence of brood pathogens (Paenibacilus larvae, Melissococus plutonius, Ascosphera
apis, Sacbrood virus, trypanosomatids Lotmaria passim and Crithidia mellificae) and
adult bees (acute bee paralysis virus, chronic bee paralysis virus, deformed bee wings
virus, trypanosomatids Lotmaria passim and Crithidia mellificae, microsporidia
Nosema apis and Nosema ceranae) as well as for determination of oxidative stress
levels by analysis of activity of enzymes: SOD, CAT, GST and concentration of
malondialdehyde (MDA). From the same hives, samples were taken to estimate the
expression the GOX gene as a parameter of social immunity. Pathogen detection was
performed by bacterial isolation as well as using PCR and real-time PCR, in accordance
with the OIE standards. The results showed that among commercially reared colonies,
P. larvae, was represented in 16.67% of samples, A. apis in 15.83%, while SBV was
detected in 96.67% of samples. However, in the brood from colonies raised in
traditional hives, only SBV in the percentage of 33.33% of samples was found. Further
research found that in adult bees from commercially reared colonies, viruses ABPV,
CBPV and DWV (83.33%, 100.00%, 100.00%, respectively), were significantly (p
<0.001) more represented compared to adult bees from „trmka― hives, where the
percentage of these viruses was 33.33% for each. Also, all commercially reared colonies
were infected with at least one of the monitored pathogens, in contrast to traditionally
reared bees, of which 66.66% were pathogen-free.
The results revealed significant differences of activities of CAT, GST and SOD
(p<0.01) and MDA concentration (p<0.002) between commercial and traditional
colonies, which leads to the conclusion that colonies grown in „trmka― hives had less
oxidative stress level, which resulted in lower presence of all monitored pathogens.
In samples of bees from commercially bred colonies, the prevalence of parasites L.
passim and N. ceranae was significantly higher (p<0.05; p<0.01, respectively)
compared to samples from traditional hives, while C. mellificae and N. apis were not
detected in any sample. It should be noted that L. passim was detected for the first time
in a brood from both groups of examined hives, commercial and traditional, with its
presence in the brood significantly lower (p<0.01) than in adult bees in commercial
colonies, while in traditionally reared bees, the presence of this trypanosome did not
differ significantly between adult bees and brood. In commercially bred bees, a
significantly higher level of mRNA for the GOX gene was found (p<0.01) compared to
traditionally bred colonies, which is probably a consequence of the increased need of
the former to strengthen social immunity. Compared to traditional beekeeping,
commercial colonies were under greater oxidative stress, and had a higher load of bee
pathogens as well as a higher level of transcription for the GOX gene, which is probably
due to frequent beekeeping and harassment of commercial colonies, as well as their
forced economic exploitation. All this leads to the conclusion that the population of
bees raised in the traditional way is more self-sustaining and resistant to bee pathogens,
energetic (and oxidative) stress, and that the anthropogenic factors (beekeeping
practices) have a negative impact on the health of commercially reared bees.
PB  - Beograd : Fakultet veterinarske medicine
T2  - 2022
T1  - Komparativno ispitivanje prisustva uzročnika i bolesti pčelinjeg legla tradicionalno i savremeno gajenih pčelinjih društava uz analizu uticaja nekih bioloških i antropogenih faktora
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3360
ER  - 

@phdthesis{
author = "Tarić, Elmin",
year = "2022",
abstract = "Doktroska disertacija, Štetoĉine i patogeni predstavljaju najĉešće uzroke gubitaka pĉelinjih društva.
Ameriĉka truleţ (AFB) je bolest pĉelinjeg legla, uzrokovana bakterijom Paenibacillus.
larvae, White (1906), moţe se smatrati glavnom pretnjom po zdravlje pĉela, s obzirom
da je reĉ o panzootskoj bolesti koja se vrlo brzo širi, ne samo iz košnice u košnicu, sa
pĉelinjaka na pĉelinjak iz regije u regiju, već i iz drţave u drţavu i šire. Evropska truleţ
je bakterijska bolest pĉelinjeg legla uzrokovana sa više bakterisjkih vrsta gde dominira
Melissococcus plutonius. Ova bolest je jako raširena i predstavlja veliki problem u
pĉelarstvu smanjujući proizvodne rezulte pĉelinjih zajednica. Kreĉno leglo (CHB) je
infekcija izazvana gljivicom Ascosphaera apis izazivajući gubitke komercijalno gajenih
pĉelinjih zajednica, naroĉito u kombinaciji sa vrstama mikrosporidija roda Nosema i
virusom mešinastog legla. Nozemoza je najĉešća bolest meĊu odraslim pĉelama
uzrokovana mirkosporidijama Nosema apis i N. ceranae i ĉesto dovodi do ekonomskih
gubitaka u pĉelarstvu. Virusne infekcije pĉela, koje su već odavno dostigle razmere
panzootije, ugroţavaju zdravlje pĉela i predstavljaju konaĉne egzekutore pĉelinjih
zajdnica. Pĉelinji krpelj Varroa destructor je glavni vektor skoro svih, a naroĉito
virusnih infekcija pĉela, koje su postale ozbiljan problem ne samo za komercijalno
gajene, već i za pĉelinje zajednice u divljini, zahvaljujući upravo krpelju V. destructor
kao fiziĉkom i biološkom vektoru Na osnovu svega navedenog jasno je da je
zdravstveno stanje društava u komercijalnom pĉelarstvu izloţeno velikim rizicima, a
njihovo ispitivanje i tretman predstavljaju veliki izazov kako za istraţivaĉe tako i za
pĉelare.
Bolesti odraslih pĉela i pĉelinjeg legla u komercijalnom pĉelarstvu najĉešće su
posledica energetskog stresa, nastalog zbog neadekvatne prihrane pĉelinjih zajednica,
prvenstveno prevelikom upotrebom sećernog sirupa. U osnovi energetskog stresa je
oksidativni stres pĉelinjih zajednica koji se moţe definisati kao disbalans izmeĊu
proizvodnje reaktivnih oblika kiseonika i antioksidativne odbrane. Reaktivni oblici
kiseonika (ROS) negativno utiĉu na ćelijske funkcije i stvaraju se tokom oksidoredukcionih metaboliĉkih procesa u ćeliji. Oni su ukljuĉeni u regulaciju razliĉitih
mehanizama, intercelularne signalizacije, a imaju i baktericidno dejstvo.
Medonosne pĉele, kao i ostale ţivotinje, razvile su niz enzimskih mehanizama
kojima se odupiru oksidativnom stresu, uklanjajući slobodne radikale. U ovim
procesima vaţnu ulogu umaju: superoksid dismutaza (SOD), enzim prisutan u citosolu i
mitohondrijama; katalaza (CAT) prisutna u peroksizomima glutation S-transferaza
(GST), glutation S transferaza (GST), peroksidaza i tireodoksin/tireoreduktazni sistem.
Imunitet pĉela, kao i imunitet u opšte, obuhvata kompleksan sistem koji ima cilj da
obezbedi oĉuvanje zdravlja kao i opstanak organizma na osnovu mnogih mehanizama
odbrane od patogena i drugih štetnih noksi (fiziĉkih i hemijskih). Medonosna pĉela svoj
socijalni imunitet ostvaruje uz pomoć bihejviorlanih mehanizama koji pomaţu zajdnici
u odbrani od patogena. Ulaganje u individualni imunitet ima visoku materijalnoenergetsku cenu za društvo, pa su udruţivanjem pĉela (razvoj socijlanog ustrojstva)
uspostavljeni zajedniĉki socijalni mehanizmi odbrane koji omogućavaju pĉelinjem
društvu efikasniju i ekonomiĉniju borbu protiv agenasa spoljašnje sredine. Za socijalni
imunitet pĉela od znaĉaja je enzim glukoza oksidazi (GOX). Ovaj enzim je produkt
pĉelinjih egzokrinih ţlezda, a uloga mu je u „konzervaciji― pĉelinjuh proizvoda, meda i
perge, tako spreĉavajući njihovo kvarenje. GOX uĉestvuje u procesu katalize -D
glukoze do glukonske kiseline i vodonik-peroksida. Vodonik-peroksid ima antiseptiĉka
svojstva, tako doprinoseći dodatnoj zaštiti i socijalnom imunitetu pĉela.
Cilj istraţivanja je bio da se utvrdi: da li izmeĊu tradicionalno i savremeno
gajenih pĉelinjih društava postoje razlike u pogledu prisustva patogena i pojave bolesti
pĉelinjeg legla, uz analizu bioloških (ekspresija GOX gena i faktora oksidativnog
stresa) i antropogenih faktora (menadţment u pĉelarstvu) na pojavu praćenih bolesti
pĉelinjeg legla i odraslih pĉela.
Terenski deo istraţivanja je obavljen na prostoru Pešterske visoravni (opština
Sjenica, Srbija) na 144 asimptomatske pĉelinje zajednice. Uzorci odraslih pĉela i
pĉelinjeg legla su uzeti iz savremenih (DB) košnica i tradicionalnih košnica „trmki―,
kako bi se utvrdila zastupljenost patogena legla (Paenibacilus larvae, Melissococus
plutonius, Ascosphera apis, virus mešinastog legla, tripanozoma Lotmaria passim i
Crithidia mellificae) i odraslih pĉela (virus akutne paralize pĉela, virus hroniĉne
paralize pĉela, virus deformisanih krila pĉela, tripanozoma Lotmaria passim i Crithidia
mellificae,mikrosporidija Nosema apis i Nosema ceranae ) kao i za utvrĊivanje nivoa
oksidativnog stresa analizom aktivnosti enzima: SOD, CAT, GST i koncentracije
malondialdehida (MDA). TakoĊe, iz istih košnica uzeti su uzorci za procenu ekspresije
GOX gena kao parametra socijlanog imuniteta.
Detekcija patogena je obavljena izolacijom bakterija kao i upotrebom PCR i
real-time PCR, u skladu sa standardima OIE. Rezultati istraţivanja su pokazali da je
meĊu komercijalno gajenim društvima P. larvae bio zastupljen u 16,67% uzoraka, A.
apis bio prisutan u 15,83% uzorka, dok je SBV detektovan u 96,67% uzoraka. MeĊutim
u leglu pĉela gajenih u tradicionalnim trmka košnicama naĊen je samo SBV u 33,33%
uzoraka. Dalja istraţivanja su utvrdila da su kod odraslih pĉela u komercijalno gajnim
društvima bili signifikantno (p<0,001) više zastupljeni virusi: ABPV, CBPV i DWV
(83,33%, 100,00%, 100,00%, istim redom) u odnosu na društva iz trmki gde je procenat
ovih virusa iznosio 33,33% za svaki virus. TakoĊe, sva komercijalno gajena društva bila
su inficirana makar jednim od praćenih patogena, za razliku od tradicionalno gajenih
pĉela u trmkama meĊu kojima je 66,66% bilo bez patogena.
Rezultati su otkrili znaĉajne razlike u aktivnosti CAT, GST i SOD (p<0,01) i
koncentracije MDA (p<0,002) izmeĊu komercijalnih i tradicionalnih društava što vodi
zakljuĉku da u društvima gajenim u trmkama postoji manji oksidativni stres što je
rezultiralo i manjom zastupljenošću svih praćenih patogena. U uzorcima komercijalno
gajenih pĉela zastupljenost parazita L. passim i N. ceranae bila je znaĉajno veća
(p<0,05; p<0,01, respektivno) u odnosu na uzorke iz tradicionalnih košnica, dok C.
mellificae i N. apis nisu detektovane ni u jednom uzorku. Treba istaći, da je prvi put
detektovana tripanozoma L. passim u leglu iz obe grupe ispitivanih košnica,
komercijalnih i tradicionalnih, pri ĉemu je njena zastupljenost u leglu znaĉajno manja
(p<0,01) nego u odraslim pĉelama u komercijalnim društvima, dok se kod tradicionalno
gajenih pĉela zastupljenost ove tripanozome izmeĊu odraslih pĉela i legla nije znaĉajno
razlikovala. Kod komercijalno gajenih pĉela utvrĊen je znaĉajno veći nivo iRNK za gen
GOX (p<0,01) u odnosu na tradicionalno gajena društva, što je verovatno posledica
pojaĉane potrebe prvopomenutih da ojaĉaju socijalni imunitet.
Komercijalna društva su u odnosu na tradicionalno gajena bila pod većim
oksidativnim stresom, a imala su i veću opterećenost pĉelinjim patogenima, kao i veći
nivo transkirpcije GOX gena, što je verovatno posledica ĉestih pĉelarskih manipulacija i
uznemiravanja komercijalnim društvima, kao i njihovo forsirano ekonomsko
iskorišćavanje. Sve to vodi zakljuĉku da populacija pĉela koja se uzgaja na
tradicionalan ima veći kapacitet samoodrţanja i otpornija na pĉelinje patogene,
energetski (i oksidativni) stres, a da antropogeni faktori, odnosno pĉelarski postupci,
imaju negativan uticaj na zdravlje komercijalno gajenih pĉela., Pests and pathogens are the most common causes of honey bee colony losses. American
foulbrood (AFB) is a disease of honey bee brood, caused by the bacterium
Paenibacillus larvae, White (1906), can be considered a major threat to bee health, as it
is a panzootic disease that spreads very quickly, not only from hive to hive, from apiary
to apiary, from region to region, but also from the state to the state and beyond.
European foulbrood is a bacterial disease of honey bee brood caused by several
bacterial species dominated by Melissococcus plutonius. This disease is widespread and
poses a major problem in beekeeping by reducing the production results of honey bee
colonies. Chalkbrood disease (CHB) is an infection caused by the fungus Ascosphaera
apis causing losses of commercially reared bee colonies, especially in combination with
microsporidia species of the genus Nosema sp. and a Sacbrood virus (SBV). Nosemosis
is the most common disease among adult bees caused by Nosema apis and N. ceranae,
and often leads to economic losses in beekeeping. Viral infections of bees, which have
long since reached panzootic proportions, endanger the health of bees and present
ultimate executors of bee colonies. Varroa destructor mite is the main vector of almost
all, especially viral infections, which have become a serious problem not only for
commercially reared, but also for honey bees in the wild, thanks to the mite V.
destructor as a physical and biological vector. It is clear that the health status of
colonies in commercial beekeeping is exposed to great risks, and their examination and
treatment represent a great challenge for both researchers and beekeepers.
Diseases of adult bees and bee brood in commercial beekeeping are most often a
consequence of energetic stress, caused by inadequate feeding of bee colonies, primarily
by excessive use of sugar syrup. The basis of energetic stress is oxidative stress of bee
colonies, which can be defined as an imbalance between oxidants and antioxidants in
favor of the former. Reactive oxygen species (ROS) negatively affect cellular functions
and are generated during oxido-reduction metabolic processes in the cell. They are
involved in the regulation of various mechanisms, intercellular signaling, and exert
bactericidal activity.
Honey bees, like other animals, have developed a number of enzymatic mechanisms
that resist oxidative stress, removing free radicals. An important role in these processes
is played by: superoxide dismutase (SOD), catalase (CAT) in peroxisomes, ascorbate
peroxidase, glutathione S transferase (GST) peroxidase, and the thyrodoxin /
thyroreductase system. Immunity of bees, as well as immunity in general, includes a
complex system that aims to ensure the preservation of health and survival of the
organism based on many defense mechanisms against pathogens and other harmful
noxa (physical and chemical). The honey bee achieves its social immunity with the help
of behavioral mechanisms that help the community defend itself against pathogens.
Investing in individual immunity has a high material and energy price for society, so the
association of bees (development of social structure) established joint social defense
mechanisms that enable bee society to fight more efficiently and economically against
environmental agents. The enzyme glucose oxidase (GOX) is important for the social
immunity of bees. This enzyme is a product of bee exocrine glands, and its role is in the
"conservation" of bee products, honey and bee bread, thus preventing their spoilage.
GOX participates in the process of catalysis of -D glucose to gluconic acid and
hydrogen peroxide. Hydrogen peroxide has antiseptic properties, thus contributing to
additional protection and social immunity of bees.
The aim of the research was to determine: whether there are differences between
traditional and modern bee colonies in terms of the presence of pathogens and the
occurrence of bee brood diseases, with the analysis of biological (expression level of the
GOX gene and oxidative stress factors) and anthropogenic factors (beekeeping
management) on monitored bee brood and adult bee diseases. The field part of the
research was performed in the area of the Peshterska plateau (Sjenica municipality,
Serbia) on 144 asymptomatic bee communities. Samples of adult bees and bee brood
were taken from modern (DB) hives and traditional "trmka" hives to determine the
presence of brood pathogens (Paenibacilus larvae, Melissococus plutonius, Ascosphera
apis, Sacbrood virus, trypanosomatids Lotmaria passim and Crithidia mellificae) and
adult bees (acute bee paralysis virus, chronic bee paralysis virus, deformed bee wings
virus, trypanosomatids Lotmaria passim and Crithidia mellificae, microsporidia
Nosema apis and Nosema ceranae) as well as for determination of oxidative stress
levels by analysis of activity of enzymes: SOD, CAT, GST and concentration of
malondialdehyde (MDA). From the same hives, samples were taken to estimate the
expression the GOX gene as a parameter of social immunity. Pathogen detection was
performed by bacterial isolation as well as using PCR and real-time PCR, in accordance
with the OIE standards. The results showed that among commercially reared colonies,
P. larvae, was represented in 16.67% of samples, A. apis in 15.83%, while SBV was
detected in 96.67% of samples. However, in the brood from colonies raised in
traditional hives, only SBV in the percentage of 33.33% of samples was found. Further
research found that in adult bees from commercially reared colonies, viruses ABPV,
CBPV and DWV (83.33%, 100.00%, 100.00%, respectively), were significantly (p
<0.001) more represented compared to adult bees from „trmka― hives, where the
percentage of these viruses was 33.33% for each. Also, all commercially reared colonies
were infected with at least one of the monitored pathogens, in contrast to traditionally
reared bees, of which 66.66% were pathogen-free.
The results revealed significant differences of activities of CAT, GST and SOD
(p<0.01) and MDA concentration (p<0.002) between commercial and traditional
colonies, which leads to the conclusion that colonies grown in „trmka― hives had less
oxidative stress level, which resulted in lower presence of all monitored pathogens.
In samples of bees from commercially bred colonies, the prevalence of parasites L.
passim and N. ceranae was significantly higher (p<0.05; p<0.01, respectively)
compared to samples from traditional hives, while C. mellificae and N. apis were not
detected in any sample. It should be noted that L. passim was detected for the first time
in a brood from both groups of examined hives, commercial and traditional, with its
presence in the brood significantly lower (p<0.01) than in adult bees in commercial
colonies, while in traditionally reared bees, the presence of this trypanosome did not
differ significantly between adult bees and brood. In commercially bred bees, a
significantly higher level of mRNA for the GOX gene was found (p<0.01) compared to
traditionally bred colonies, which is probably a consequence of the increased need of
the former to strengthen social immunity. Compared to traditional beekeeping,
commercial colonies were under greater oxidative stress, and had a higher load of bee
pathogens as well as a higher level of transcription for the GOX gene, which is probably
due to frequent beekeeping and harassment of commercial colonies, as well as their
forced economic exploitation. All this leads to the conclusion that the population of
bees raised in the traditional way is more self-sustaining and resistant to bee pathogens,
energetic (and oxidative) stress, and that the anthropogenic factors (beekeeping
practices) have a negative impact on the health of commercially reared bees.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "2022",
title = "Komparativno ispitivanje prisustva uzročnika i bolesti pčelinjeg legla tradicionalno i savremeno gajenih pčelinjih društava uz analizu uticaja nekih bioloških i antropogenih faktora",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3360"
}

Tarić, E.. (2022). Komparativno ispitivanje prisustva uzročnika i bolesti pčelinjeg legla tradicionalno i savremeno gajenih pčelinjih društava uz analizu uticaja nekih bioloških i antropogenih faktora. in 2022
Beograd : Fakultet veterinarske medicine..
https://hdl.handle.net/21.15107/rcub_veterinar_3360

Tarić E. Komparativno ispitivanje prisustva uzročnika i bolesti pčelinjeg legla tradicionalno i savremeno gajenih pčelinjih društava uz analizu uticaja nekih bioloških i antropogenih faktora. in 2022. 2022;.
https://hdl.handle.net/21.15107/rcub_veterinar_3360 .

Tarić, Elmin, "Komparativno ispitivanje prisustva uzročnika i bolesti pčelinjeg legla tradicionalno i savremeno gajenih pčelinjih društava uz analizu uticaja nekih bioloških i antropogenih faktora" in 2022 (2022),
https://hdl.handle.net/21.15107/rcub_veterinar_3360 .

TY  - JOUR
AU  - Davitkov, Dajana
AU  - Vučićević, Miloš
AU  - Glavinić, Uroš
AU  - Skadric, Ivan
AU  - Nešić, Vladimir
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2134
AB  - Nowadays, illegal trafficking and smuggling of animals are among the greatest threats to many avian species. Most commonly smuggled birds belong to parrots, song birds, raptor species and owls. All of these species are protected by national and international legislations. In order to prevent and reduce wildlife trafficking, DNA methods have become an important forensic tool in species and sex identification. In this study, CHD1R/CHD1F primer pair was used to amplify a part of the CHD1 gene from 65 birds that belong to 43 species. For 36 species this is the first time that the length of CHD1 amplicons was measured for the purpose of species determination. The results were visualized using capillary electrophoresis and enabled simultaneous determination of sex and species. Based on the number of amplicons (two in females, one in males) sex was successfully determined in all species, even in cases where gel electrophoresis failed to give results. Moreover, the species was successfully determined in most bird species based on the species-specific sizes of CHD1 amplicon. The method used in this study is of great importance for veterinary forensic medicine and the prevention of wildlife smuggling. Still, further work is necessary to confirm the effectiveness of the method in all bird species.
AB  - Ilegalna trgovina i krijumčarenje divljim vrstama danas predstavlja jednu od najvećih pretnji koje ugrožavaju populacije velikog broja ptica. Najčešće vrste kojima se ilegalno trguje su papagaji, ptice pevačice, grabljivice i sove. Sve ove vrste ptica su zaštićene nacionalnim ili međunarodnim zakonskim regulativama. U cilju prevencije i smanjenja ilegalne trgovine, molekularne metode su postale bitno forenzičko oruđe u identifi kaciji vrste i pola zaštićenih vrsta ptica. U ovom istraživanju ispitano je ukupno 65 uzoraka ptica (ukupno 43 vrste), a u cilju umnožavanja dela CHD1 gena korišćen je CHD1R/CHD1F par prajmera. Za 36 vrsta ovo je prvi put da je dužina CHD1 gena određivana u cilju identifi kacije vrste. Dobijeni produkti su vizuelizovani koristeći kapilarnu elektroforezu i omogućili su simultano određivanje pola i vrste. Na osnovu analize amplikona (dva kod ženke, jedan kod mužjaka), pol je uspešno identifi kovan kod svih vrsta, čak i kod onih kod kojih gel elektroforeza nije bila uspešna.Vrsta je uspešno određena kod većine ptica na osnovu veličine CHD1 amplikona specifične za vrstu. Metoda korišćena u ovoj studiji je od velikog značaja u veterinarskoj forenzici i prevenciji ilegalne trgovine životinjama. Ipak, dalji rad na ovoj metodi je neophodan da bi se potvrdila efi kasnost metode na svim vrstama ptica.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Potential of inter- and intra-species variability of CHD1 gene in birds as a forensic tool
T1  - Mogućnost upotrebe interspecijske i intraspecijske varijabilnosti chd1 gena ptica u forenzici
VL  - 71
IS  - 2
SP  - 147
EP  - 157
DO  - 10.2478/acve-2021-0013
ER  - 

@article{
author = "Davitkov, Dajana and Vučićević, Miloš and Glavinić, Uroš and Skadric, Ivan and Nešić, Vladimir and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2021",
abstract = "Nowadays, illegal trafficking and smuggling of animals are among the greatest threats to many avian species. Most commonly smuggled birds belong to parrots, song birds, raptor species and owls. All of these species are protected by national and international legislations. In order to prevent and reduce wildlife trafficking, DNA methods have become an important forensic tool in species and sex identification. In this study, CHD1R/CHD1F primer pair was used to amplify a part of the CHD1 gene from 65 birds that belong to 43 species. For 36 species this is the first time that the length of CHD1 amplicons was measured for the purpose of species determination. The results were visualized using capillary electrophoresis and enabled simultaneous determination of sex and species. Based on the number of amplicons (two in females, one in males) sex was successfully determined in all species, even in cases where gel electrophoresis failed to give results. Moreover, the species was successfully determined in most bird species based on the species-specific sizes of CHD1 amplicon. The method used in this study is of great importance for veterinary forensic medicine and the prevention of wildlife smuggling. Still, further work is necessary to confirm the effectiveness of the method in all bird species., Ilegalna trgovina i krijumčarenje divljim vrstama danas predstavlja jednu od najvećih pretnji koje ugrožavaju populacije velikog broja ptica. Najčešće vrste kojima se ilegalno trguje su papagaji, ptice pevačice, grabljivice i sove. Sve ove vrste ptica su zaštićene nacionalnim ili međunarodnim zakonskim regulativama. U cilju prevencije i smanjenja ilegalne trgovine, molekularne metode su postale bitno forenzičko oruđe u identifi kaciji vrste i pola zaštićenih vrsta ptica. U ovom istraživanju ispitano je ukupno 65 uzoraka ptica (ukupno 43 vrste), a u cilju umnožavanja dela CHD1 gena korišćen je CHD1R/CHD1F par prajmera. Za 36 vrsta ovo je prvi put da je dužina CHD1 gena određivana u cilju identifi kacije vrste. Dobijeni produkti su vizuelizovani koristeći kapilarnu elektroforezu i omogućili su simultano određivanje pola i vrste. Na osnovu analize amplikona (dva kod ženke, jedan kod mužjaka), pol je uspešno identifi kovan kod svih vrsta, čak i kod onih kod kojih gel elektroforeza nije bila uspešna.Vrsta je uspešno određena kod većine ptica na osnovu veličine CHD1 amplikona specifične za vrstu. Metoda korišćena u ovoj studiji je od velikog značaja u veterinarskoj forenzici i prevenciji ilegalne trgovine životinjama. Ipak, dalji rad na ovoj metodi je neophodan da bi se potvrdila efi kasnost metode na svim vrstama ptica.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Potential of inter- and intra-species variability of CHD1 gene in birds as a forensic tool, Mogućnost upotrebe interspecijske i intraspecijske varijabilnosti chd1 gena ptica u forenzici",
volume = "71",
number = "2",
pages = "147-157",
doi = "10.2478/acve-2021-0013"
}

Davitkov, D., Vučićević, M., Glavinić, U., Skadric, I., Nešić, V., Stevanović, J.,& Stanimirović, Z.. (2021). Potential of inter- and intra-species variability of CHD1 gene in birds as a forensic tool. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(2), 147-157.
https://doi.org/10.2478/acve-2021-0013

Davitkov D, Vučićević M, Glavinić U, Skadric I, Nešić V, Stevanović J, Stanimirović Z. Potential of inter- and intra-species variability of CHD1 gene in birds as a forensic tool. in Acta Veterinaria-Beograd. 2021;71(2):147-157.
doi:10.2478/acve-2021-0013 .

Davitkov, Dajana, Vučićević, Miloš, Glavinić, Uroš, Skadric, Ivan, Nešić, Vladimir, Stevanović, Jevrosima, Stanimirović, Zoran, "Potential of inter- and intra-species variability of CHD1 gene in birds as a forensic tool" in Acta Veterinaria-Beograd, 71, no. 2 (2021):147-157,
https://doi.org/10.2478/acve-2021-0013 . .

TY  - JOUR
AU  - Jovanović, Nemanja M.
AU  - Glavinić, Uroš
AU  - Delić, Biljana
AU  - Vejnović, Branislav
AU  - Aleksić, Nevenka
AU  - Mlađan, Vladimir
AU  - Stanimirović, Zoran
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2032
AB  - It is common knowledge that nutritive stress resulting from decreased diversity and quality of food, pollution of food sources and beekeeping errors may lead to increased susceptibility of bees to pathogens and pesticides. The dearth of adequate food is frequently compensated with supplements. Thus, this research was aimed to study the effects of the plant-based supplement B + on colony strength (assessed according to open and sealed brood area, honey and pollen/bee bread reserves, and the number of adult bees). In addition, Nosema ceranae spores and viruses were quantified and the level of infestation with Varroa destructor assessed. The experiment was conducted in late summer and early spring. In colonies which were given B + in feed a significant increase (p < 0.05) in the parameters of colony strength were noticed in comparison to the control (colonies fed on sugar syrup). Moreover, it was proven that the bees from these colonies had significantly lower (p < 0.05) N. ceranae spore counts, and acute bee paralysis, deformed wing and sacbrood virus loads. Our results suggest that the addition of B + supplement to the colonies provide them with nutrients, contribute to their strengthening, might prevent nutritive stress and increase the success of bees in combating pathogens.
PB  - Elsevier
T2  - Preventive Veterinary Medicine
T1  - Plant-based supplement containing B-complex vitamins can improve bee health and increase colony performance
VL  - 190
SP  - 105322
DO  - 10.1016/j.prevetmed.2021.105322
ER  - 

@article{
author = "Jovanović, Nemanja M. and Glavinić, Uroš and Delić, Biljana and Vejnović, Branislav and Aleksić, Nevenka and Mlađan, Vladimir and Stanimirović, Zoran",
year = "2021",
abstract = "It is common knowledge that nutritive stress resulting from decreased diversity and quality of food, pollution of food sources and beekeeping errors may lead to increased susceptibility of bees to pathogens and pesticides. The dearth of adequate food is frequently compensated with supplements. Thus, this research was aimed to study the effects of the plant-based supplement B + on colony strength (assessed according to open and sealed brood area, honey and pollen/bee bread reserves, and the number of adult bees). In addition, Nosema ceranae spores and viruses were quantified and the level of infestation with Varroa destructor assessed. The experiment was conducted in late summer and early spring. In colonies which were given B + in feed a significant increase (p < 0.05) in the parameters of colony strength were noticed in comparison to the control (colonies fed on sugar syrup). Moreover, it was proven that the bees from these colonies had significantly lower (p < 0.05) N. ceranae spore counts, and acute bee paralysis, deformed wing and sacbrood virus loads. Our results suggest that the addition of B + supplement to the colonies provide them with nutrients, contribute to their strengthening, might prevent nutritive stress and increase the success of bees in combating pathogens.",
publisher = "Elsevier",
journal = "Preventive Veterinary Medicine",
title = "Plant-based supplement containing B-complex vitamins can improve bee health and increase colony performance",
volume = "190",
pages = "105322",
doi = "10.1016/j.prevetmed.2021.105322"
}

Jovanović, N. M., Glavinić, U., Delić, B., Vejnović, B., Aleksić, N., Mlađan, V.,& Stanimirović, Z.. (2021). Plant-based supplement containing B-complex vitamins can improve bee health and increase colony performance. in Preventive Veterinary Medicine
Elsevier., 190, 105322.
https://doi.org/10.1016/j.prevetmed.2021.105322

Jovanović NM, Glavinić U, Delić B, Vejnović B, Aleksić N, Mlađan V, Stanimirović Z. Plant-based supplement containing B-complex vitamins can improve bee health and increase colony performance. in Preventive Veterinary Medicine. 2021;190:105322.
doi:10.1016/j.prevetmed.2021.105322 .

Jovanović, Nemanja M., Glavinić, Uroš, Delić, Biljana, Vejnović, Branislav, Aleksić, Nevenka, Mlađan, Vladimir, Stanimirović, Zoran, "Plant-based supplement containing B-complex vitamins can improve bee health and increase colony performance" in Preventive Veterinary Medicine, 190 (2021):105322,
https://doi.org/10.1016/j.prevetmed.2021.105322 . .

TY  - JOUR
AU  - Rajković, Milan
AU  - Glavinić, Uroš
AU  - Ristanić, Marko
AU  - Ćosić, Milivoje
AU  - Dimitrijević-Srećković, Vesna
AU  - Ilić, Iva
AU  - Đelić, Ninoslav
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2239
AB  - Diabetes is one of the main health concerns, especially in developed countries. During the last few decades, the percentage of diabetic persons is constantly increasing. Although the genetic factors have a strong influence in the development of diabetes, environmental influence (physical inactivity, inadequate nutrition leading to obesity) also have an important impact. The main objective of this investigation was to evaluate the possible influence of organic sprouted whole wheat grain flourless bread Tonus® (product of Trivit, Becej, Serbia) on DNA damage at various stages of progression through type 2 diabetes mellitus (T2DM). In addition to control (non-diabetic) subjects we analyzed obese, pre-diabetic and diabetic patients, for a total of four experimental groups. All subjects used to eat Mediterranean diet for at least two years before being included in our study. In each of four groups we had five persons practicing the Mediterranean diet but instead of bread they consumed flourless bread Tonus®. The DNA damage was evaluated on peripheral blood mononuclear cells by alkaline single cell gel electrophoresis (Comet) assay at the very beginning (before starting the Tonus® bread diet), and after exactly the three months of consumption of Tonus® bread. Statistical analysis revealed that only in patients with type
AB  - Dijabetes predstavlja jedan od glavnih zdravstvenih problema, naročito u razvijenim zemljama. Tokom poslednjih nekoliko decenija neprekidno se povećava procenat osoba obolelih od dijabetesa. Mada genetički faktori imaju snažan uticaj na razvoj dijabetesa, uticaji sredine (fizička neaktivnost, nepravilna ishrana koja dovodi do gojaznosti) takođe imaju veliki uticaj na razvoj ove bolesti. Tokom nekoliko poslednjih decenija procenat osoba sa dijabetesom se konstantno povećava. Glavni cilj ovog istraživanja bio je da se proceni mogući uticaj organskog hleba bez brašna od celih proklijalih zrna pšenice, Tonus® (Trivit, Bečej, Serbia) na oštećenja DNK u različitim stadijumima progresije kroz tip 2 dijabetes melitus. Pored kontrolne grupe (zdrave osobe), analizirali smo gojazne, predijabetičare i dijabetičare, što čini ukupno četiri eksperimentalne grupe. Sve osobe su koristile Mediteransku ishranu najmanje dve godine pre nego su uključene u ovu studiju. U svakoj grupi imali smo pet osoba koje su upražnjavale Mediteransku ishranu, ali su umesto uobičajenog hleba konzumirali hleb bez brašna Tonus®. Oštećenja DNK su evaluirana alkalnom verzijom elektoroforeze DNK pojedinačnih ćelija (komet test) na samom početku (pre uvođenja hleba Tonus® u ishranu) i tačno nakon tri meseca konzumiranja hleba Tonus®. Statistička analiza otkrila je da samo kod pacijenata sa dijabetesom tipa 2 konzumiranje Tonus hleba dovodi do smanjenja oštećenja DNK u poređenju sa stepenom oštećenja DNK kod ovih pacijenata pre nego su otpočeli dijetu sa hlebom Tonus. Pretpostaljamo da je pad hiperinsulinemije usled konzumiranja hleba Tonus mogao da bude jedan od glavnih uzroka smanjenja oštećenja DNK.
PB  - Faculty of Veterinary Medicine, Belgrade University
T2  - Acta Veterinaria
T2  - Acta Veterinaria-Beograd
T1  - Does organic sprouted whole wheat grain flourless bread decreases DNA damage in diabetic patients?
T1  - Da li organski hleb bez brašna od celih proklijalih zrna pšenice smanjuje oštećenja DNK kod pacijenata sa dijabetesom?
VL  - 71
IS  - 3
SP  - 273
EP  - 284
DO  - 10.2478/acve-2021-0024
ER  - 

@article{
author = "Rajković, Milan and Glavinić, Uroš and Ristanić, Marko and Ćosić, Milivoje and Dimitrijević-Srećković, Vesna and Ilić, Iva and Đelić, Ninoslav",
year = "2021",
abstract = "Diabetes is one of the main health concerns, especially in developed countries. During the last few decades, the percentage of diabetic persons is constantly increasing. Although the genetic factors have a strong influence in the development of diabetes, environmental influence (physical inactivity, inadequate nutrition leading to obesity) also have an important impact. The main objective of this investigation was to evaluate the possible influence of organic sprouted whole wheat grain flourless bread Tonus® (product of Trivit, Becej, Serbia) on DNA damage at various stages of progression through type 2 diabetes mellitus (T2DM). In addition to control (non-diabetic) subjects we analyzed obese, pre-diabetic and diabetic patients, for a total of four experimental groups. All subjects used to eat Mediterranean diet for at least two years before being included in our study. In each of four groups we had five persons practicing the Mediterranean diet but instead of bread they consumed flourless bread Tonus®. The DNA damage was evaluated on peripheral blood mononuclear cells by alkaline single cell gel electrophoresis (Comet) assay at the very beginning (before starting the Tonus® bread diet), and after exactly the three months of consumption of Tonus® bread. Statistical analysis revealed that only in patients with type, Dijabetes predstavlja jedan od glavnih zdravstvenih problema, naročito u razvijenim zemljama. Tokom poslednjih nekoliko decenija neprekidno se povećava procenat osoba obolelih od dijabetesa. Mada genetički faktori imaju snažan uticaj na razvoj dijabetesa, uticaji sredine (fizička neaktivnost, nepravilna ishrana koja dovodi do gojaznosti) takođe imaju veliki uticaj na razvoj ove bolesti. Tokom nekoliko poslednjih decenija procenat osoba sa dijabetesom se konstantno povećava. Glavni cilj ovog istraživanja bio je da se proceni mogući uticaj organskog hleba bez brašna od celih proklijalih zrna pšenice, Tonus® (Trivit, Bečej, Serbia) na oštećenja DNK u različitim stadijumima progresije kroz tip 2 dijabetes melitus. Pored kontrolne grupe (zdrave osobe), analizirali smo gojazne, predijabetičare i dijabetičare, što čini ukupno četiri eksperimentalne grupe. Sve osobe su koristile Mediteransku ishranu najmanje dve godine pre nego su uključene u ovu studiju. U svakoj grupi imali smo pet osoba koje su upražnjavale Mediteransku ishranu, ali su umesto uobičajenog hleba konzumirali hleb bez brašna Tonus®. Oštećenja DNK su evaluirana alkalnom verzijom elektoroforeze DNK pojedinačnih ćelija (komet test) na samom početku (pre uvođenja hleba Tonus® u ishranu) i tačno nakon tri meseca konzumiranja hleba Tonus®. Statistička analiza otkrila je da samo kod pacijenata sa dijabetesom tipa 2 konzumiranje Tonus hleba dovodi do smanjenja oštećenja DNK u poređenju sa stepenom oštećenja DNK kod ovih pacijenata pre nego su otpočeli dijetu sa hlebom Tonus. Pretpostaljamo da je pad hiperinsulinemije usled konzumiranja hleba Tonus mogao da bude jedan od glavnih uzroka smanjenja oštećenja DNK.",
publisher = "Faculty of Veterinary Medicine, Belgrade University",
journal = "Acta Veterinaria, Acta Veterinaria-Beograd",
title = "Does organic sprouted whole wheat grain flourless bread decreases DNA damage in diabetic patients?, Da li organski hleb bez brašna od celih proklijalih zrna pšenice smanjuje oštećenja DNK kod pacijenata sa dijabetesom?",
volume = "71",
number = "3",
pages = "273-284",
doi = "10.2478/acve-2021-0024"
}

Rajković, M., Glavinić, U., Ristanić, M., Ćosić, M., Dimitrijević-Srećković, V., Ilić, I.,& Đelić, N.. (2021). Does organic sprouted whole wheat grain flourless bread decreases DNA damage in diabetic patients?. in Acta Veterinaria
Faculty of Veterinary Medicine, Belgrade University., 71(3), 273-284.
https://doi.org/10.2478/acve-2021-0024

Rajković M, Glavinić U, Ristanić M, Ćosić M, Dimitrijević-Srećković V, Ilić I, Đelić N. Does organic sprouted whole wheat grain flourless bread decreases DNA damage in diabetic patients?. in Acta Veterinaria. 2021;71(3):273-284.
doi:10.2478/acve-2021-0024 .

Rajković, Milan, Glavinić, Uroš, Ristanić, Marko, Ćosić, Milivoje, Dimitrijević-Srećković, Vesna, Ilić, Iva, Đelić, Ninoslav, "Does organic sprouted whole wheat grain flourless bread decreases DNA damage in diabetic patients?" in Acta Veterinaria, 71, no. 3 (2021):273-284,
https://doi.org/10.2478/acve-2021-0024 . .

TY  - THES
AU  - Drašković, Vladimir Lj.
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2268
AB  - Uzgoj svinja predstavlja jednu od najvažnijih grana stočarstva. Neadekvatna primena biosigurnosnih mera i loši ambijentalni uslovi, uz prisustvo patogenih prouzrokovača bolesti, mogu onemogućiti uspešnu proizvodnju svinja. U preko 50% slučajeva enterične bolesti su odgovorne za ograničavanje rentabilne proizvodnje. Jedan od važnih uzročnika koji dovodi do enteričnih poremećaja i velikih ekonomskih gubitaka je i Lawsonia intracellularis, obliganta intracelularna Gram negativna bakterija, koja prouzrokuje proliferativnu enteropatiju (PE). „Zlatni standardi“ za identifikaciju L. intracellularis su imunohistohemijska i PCR metoda. Razvojem kvantitativne real-time (qPCR) metode omogućeno je rutinsko otkrivanje subkliničkih formi bolesti, a kvantifikovanje uzročnika je omogućilo da se odredi težina kliničke slike inficiranih svinja. Zbog često pogrešne dijagnostike ove bolesti na farmama, posledično prekomerne upotrebe antibiotika i razvoja antimikrobne rezistencije uzročnika, postoji potreba za pronalaženjem alternativnih rešenja u kontroli PE. Takođe, dobre biosigurnosne mere na farmama, za koje je pokazano da pozitivno utiču na zdravlje i proizvodne rezultate svinja, mogu predstavljati bitan faktor u prevenciji nastanka PE. U skladu sa tim cilj istraživanja ove doktorske disertacije bio je ispitivanje uticaja komercijalnog fitogenog aditiva (Patente Herba® Plus) u kontroli PE kod odlučene prasadi prirodno inficirane bakterijom L. intracellularis, uspostavljanje i optimizacija protokola za molekularnogenetičku identifikaciju bakterije L. intracellularis, kao i kvantifikaciju stepena infekcije kod prasadi putem real-time qPCR i IHC metoda. Dodatno je ispitivan i uticaj različitih biosigurnosnih nivoa i Patente Herba® Plus fitogenog aditiva na proizvodne rezultate i broj izlučenih bakterija L. intracellularis u fecesu prasadi.
Eksperiment je sproveden na četiri različite farme svinja na kojima je određen nivo eksternih, internih i ukupnih biosigurnosnih mera. Na svakoj od farmi (farma BS1, farma BS2, farma BS3 i farma BS4), prasad stara sedam nedelja, ujednačenih telesnih masa, bila je raspoređena u kontrolne grupe koje u hrani nisu dobijale preparat Patente Herba® Plus (K-BS1, K-BS2, K-BS3 i K-BS4) i tretman grupe koje su hrani dobijale preparat Patente Herba® Plus u koncentraciji od 2 kg/t hrane (T-BS1, T-BS2, T-BS3 i T-BS4). Kontrolne i tretman grupe formirane su sa po 72 jedinke, raspoređene u devet bokseva sa po osam prasadi, odnosno 144 prasadi po farmi, ukupno 576 prasadi. Za potrebe histoloških analiza izveden je eksperiment sa individualno gajenom prasadi, po šest u kontrolnoj i tretman grupi. Tokom 28 dana eksperimenta (0., 14. i 28. dan) uzimani su uzorci fecesa za molekularnogenetička ispitivanja, praćeni su proizvodni rezultati prasadi (telesna masa, dnevni i ukupni prorast, dnevna i ukupna konzumacija i konverzija), dok na kraju eksperimenta su uzimani uzorci ileuma za histološke (mikroskopske promene i histomorfometrijski parametari) i imunohistohemijske analize (ekspresija antigena L. intracelullaris).
Na sve četiri farme, u okviru eksternih biosigurnosnih mera, najbolje su bile ocenjene potkategorije koje se odnose na kupovinu životinja i semena i zaposlene i posetioce, dok najniže ocene su dobile biosigurnosne mere koje se odnose na hranu, vodu i opremu. U okviru internih biosigurnosnih mera potkategorije kontrola bolesti i prasilište i period dojenja bile su najbolje ocenjene na farmama, a najlošije su bile ocenjene biosigurnosne mere koje se odnose na čišćenje i dezinfekciju, odgajivalište i mere između odeljaka i korišćenje opreme. Ukupne vrednosti biosigurnosnih mera na sve četiri farme (BS 1 60%, BS 2 64%, BS 3 77% i BS 4 86%) koje su analizirane u ovoj doktorskoj disertaciji bile su više od prosečnih vrednosti biosigurnosnih mera na farmama u Srbiji (58%) i svetu (64%), izuzev farme BS 1 čija ocena ukupnih biosigurnosnih mera je bila niža u poređenju sa prosečnim vrednostima u svetu (60%).
Na farmi BS1 utvrđen je značajno manji broj bakterija L. intracellularis u fecesu prasadi tretman grupe u odnosu na kontrolnu grupu 14. i 28. dana eksperimenta (P=0,0080 i P=0,0015, pojedinačno). Takođe, prasad tretman grupe imala je značajno manji broj bakterija L. intracellularis u fecesu 14. i 28. dana u odnosu na 0. dan eksperimenta (P<0,0001). Na farmi BS 2 uočen je značajan pozitivni efekat preparata Patente Herba® Plus u hrani tretman grupe prasadi na broj bakterija L. intracellularis 14. i 28. dana ogleda u odnosu na kontrolnu grupu (P=0,0470 i P=0,0176, pojedinačno), dok nije uočena značajna razlika u broju bakterija L. intracellularis u fecesu prasadi tretman grupe tokom trajanja eksperimenta (P=0,6174). Na farmama BS 3 i BS 4 nije uočena razlika između kontrolne i tretman grupe prasadi u broju bakterija L. intracellularis za sva tri ispitivana perioda (P>0,05), niti je uočen značajan pad broja bakterija L. intracellularis u fecesu prasadi tretman grupa tokom trajanja ogleda (P>0,05).
Uočena je značajna negativna korelaciona zavisnost (r=-0,664, P<0,01) između broja bakterija L. intracellularis u fecesu prasadi i biosigurnosnih nivoa farmi, dok nije utvrđena značajna korelaciona zavisnost između broja bakterija L. intracellularis, tretmana fitogenim aditivom i dužine trajanja tretmana (P>0,05).
Histološke promene na preparatima ileuma kontrolne i tretman grupe prasadi su deskriptivno analizirane, dok je histomorfometrijskim ispitivanjima pokazano da su u tretman grupi prasadi kripte bile značajno pliće (P=0,0284), a odnos visina resica/dubina kripti značajno veći (P=0,0040) nego u
kontrolnoj grupi. Nije uočen efekat dodavanja fitogenog aditiva u hrani prasadi na visinu resica (P=0,0607), širinu resica (P=0,0728), površinu resica (P=0,7676) i broj peharastih ćelija/100 enterocita (P=0,0575).
Semikvantitativnom analizom, na osnovu imunohistohemijskih nalaza, 33,33% uzoraka ilealnog tkiva tretman grupe prasadi bilo je ocenjeno ocenom 0, a preostalih 66,67% ocenom 1, dok je u kontrolnoj grupi 50% uzoraka bilo ocenjeno ocenom 1, 33,33% ocenom 2, a 16,67% uzoraka ocenom 3.
Primena fitogenog aditiva u hrani nije značajno uticala na telesnu masu prasadi nakon 28 dana eksperimenta na svim ispitivanim farmama (P>0,05). Ukupni i dnevni prirast prasadi tretman grupe bio je značajno veći na farmi BS 2 u poređenju sa kontrolnom grupom (P=0,0349 i P=0,0345, pojedinačno), dok na farmama BS 1, BS 3 i BS 4 razlike u ukupnom i dnevnom prirastu tretman i kontrolne grupe nisu uočene (P>0,05). Najbolji prirast je postigla grupa koja je u hrani dobijala preparat Patente Herba® Plus na farmi BS 4, koji je bio značajno viši u poređenju sa K-BS1, K-BS2 i T-BS2 grupom prasadi (P<0,0001).
Na kraju eksperimenta (28. dan) nije uočena razlika u ukupnoj i dnevnoj konzumaciji hrane između kontrolne i tretman grupe prasadi u okviru svake farme (P>0,05). Značajno bolja konverzija tretman grupe prasadi u odnosu na kontrolnu grupu utvrđena je na farmi BS 1 (P=0,0121), dok na drugim farmama nije uočena razlika u konverziji hrane prasadi kontrolne i tretman grupe (P>0,05).
Dvofaktorijalnom analizom varijanse utvđen je značajan efekat primene preparata Patente Herba® Plus i biosigurnosnih nivoa farmi na ukupan i dnevni prirast prasadi (P<0,05). Biosigurnosni nivoi na farmama su značajno uticali i na ukupnu i dnevnu konzumaciju hrane (P=0,0435 i P=0,0434, pojedinačno), dok je dodavanje Patente Herba® Plus u hrani prasadi imalo i značajan efekat na konverziju (P=0,0046). Nije uočen značajan uticaj interakcije ova dva parametra na ukupni i dnevni prirast, ukupnu i dnevnu konzumaciju i konverziju hrane prasadi tokom ogleda (P>0,05).
AB  - Pig breeding is one of the most important branches of animal husbandry. Inadequate application of biosecurity measures and poor environmental conditions, in the presence of pathogens, could disable successful pig production. In over 50% of cases, enteric diseases could effect profitable production. One of the important enteric pathogens associated with large economic losses is Lawsonia intracellularis, an obligate intracellular Gram-negative bacterium, which causes proliferative enteropathy (PE). The “gold standards” for identifying L. intracellularis are immunohistochemical and PCR methods. The development of the quantitative real-time (qPCR) method enabled the routine detection of subclinical forms of the disease, and the quantification of the bacteria gives the possibility to determine the severity of the clinical symptoms in infected pigs. Since this infection is often misdiagnosed on pig farms, this results in excessive use of antibiotics and the ability of bacteria to develop resistance to antimicrobial agents. Therefore, it is necessary to find alternative solutions in PE control. Also, good biosecurity measures on farms, for which it is shown to have positive effect on health and production results of pigs, can be an important factor in the prevention of PE. Accordingly, the aim of this doctoral dissertation was to investigate the effect of commercial phytogenic additive (Patente Herba® Plus) in PE control in weaned piglets naturally infected with L. intracellularis, to establish and optimize protocols for molecular genetic identification of L. intracellularis, as well as to quantify the degree of infection in piglets via real-time qPCR and IHC methods. Additionally, the effect of different biosecurity levels and Patente Herba® Plus phytogenic additive on the production results and the number of excreted L. intracellularis in the faeces of piglets were investigated.
The experiment was conducted on four different pig farms on which the levels of external, internal and total biosecurity measures were determined. On each of the farms (farm BS1, farm BS2, farm BS3 and farm BS4), seven-week-old piglets of uniform body weight were assigned to control groups that did not receive Patente Herba® Plus (K-BS1, K-BS2, K-BS3 and K-BS4) and the treatment groups that received Patente Herba® Plus in their feed at a concentration of 2 kg/t feed (T-BS1, T-BS2, T-BS3 and T-BS4). Control and treatment groups consisted of 72 piglets each, distributed in nine pens with eight piglets each, i.e. 144 piglets per farm, a total of 576 piglets. For histological analyzes, an additional experiment with individually reared piglets was performed, which consistet of six piglets in each, control and treatment group. During the 28 days of the experiment (days 0, 14, and 28) faeces samples
were taken for molecular genetic analysis, the production results of piglets (body weight, daily and total weight gain, daily and total consumption and feed conversion ratio) were monitored, while at the end of the experiment ileum samples were taken for histological (microscopic changes and histomorphometric parameters) and immunohistochemical analyzes (L. intracelullaris antigen expression). On all four farms, within the external biosecurity, the subcategories related to the purchase of animals and semen and personnel and visitors were rated the best, while the biosecurity measures related to the feed, water and equipment supply were rated the lowest. Within the internal biosecurity, the subcategories of disease management and farrowing and suckling period had the highest score on farms, while the lowest score had biosecurity measures related to cleaning and disinfection, nursery unit and measures between compartments and use of equipment. The total biosecurity values of all four examined farms (BS 1 60%, BS 2 64%, BS 3 77% and BS 4 86%) in this doctoral dissertation were higher than the average biosecurity values on farms in Serbia (58%) and in the world (64%), with exception of farm BS 1 where total biosecurity value was lower compared to the average values in the world (60%).
On the farm BS1, a significantly lower number of L. intracellularis was found in the faeces of piglets in the treatment group compared to the control group on days 14 and 28 of the experiment (P=0.0080 and P=0.0015, respectively). Also, the piglets of the treatment group had a significantly lower number of L. intracellularis in the faeces on days 14 and 28 compared to day 0 of the experiment (P<0.0001). On the farm BS 2, a significant positive effect of Patente Herba® Plus in the feed on the number of L. intracellularis in the treatment group of piglets was observed on the 14th and 28th day of the experiment in relation to the control group (P=0.0470 and P=0.0176, respectively), while in the treatment group alone no significant difference was observed in the number of L. intracellularis in the faeces of piglets during the experiment (P=0.6174). On the farm BS 3 and BS 4, there were no significant difference between the control and treatment groups of piglets in the number of L. intracellularis in faeces for all three examined periods (P>0.05), also a significant decrease in the number of L. intracellularis in the faeces of piglets in the treatment groups during the experiment was not observed (P>0.05).
A significant negative correlation was determined (r=-0.664, P<0.01) between the number of L. intracellularis in faeces and on-farm biosecurity levels, while no significant correlation was found between the number of L. intracellularis in faeces, the phytogenic additive treatment and the treatment duration (P>0.05).
Histological changes on ileum preparations of the control and the treatment groups of piglets were descriptively analyzed, while histomorphometric examinations showed that in the treatment group the crypts were significantly shallower (P=0.0284) and the villus height/crypt depth ratio significantly higher (P=0.0040) than in the control group. No effect of the addition of phytogenic additive was observed in piglet feed on villus height (P=0.0607), villus width (P=0.0728), villus surface area (P=0.7676) and the number of goblet cells/100 enterocytes (P=0.0575).
Using semiquantitative analysis, based on immunohistochemical findings, 33.33% of ileal tissue samples of the treatment group piglets were rated with a grade 0, and the remaining 66.67% with a grade 1, while in the control group 50% of samples were rated with a grade 1, 33.33% with grade 2, and 16.67% of samples with grade 3.
The use of phytogenic additive in feed did not significantly affect the body weight of piglets after 28 days of the experiment on all tested farms (P>0.05). The total and daily weight gain of piglets in the treatment group was significantly higher compared to the control group on the farm BS 2 (P=0.0349 and P=0.0345, respectively), while on farms BS 1, BS 3 and BS 4 the differences in total and daily weight gain in treatment and control groups of piglets were not observed (P>0.05). The best weight gain was achieved in the group that received the preparation Patente Herba® Plus in feed on farm BS 4, which was significantly higher compared to K-BS1, K-BS2 and T-BS2 group of piglets (P<0.0001).
At the end of the experiment (day 28), no differences in total and daily feed consumption were observed between the control and treatment groups of piglets within each farm (P>0.05). Significantly better feed conversion ratio of piglet in the treatment group compared to the control was found on farm BS 1 (P=0.0121), while on other farms no differences were observed in the feed conversion ratio of piglets in control and treatment groups (P>0.05).
Two-way analysis of variance revealed a significant effect of the application of Patente Herba® Plus and biosecurity levels of farms on the total and daily weight gain of piglets (P<0.05). Biosecurity levels on farms significantly affected both total and daily feed consumption (P=0.0435 and P=0.0434, respectively), while the addition of Patente Herba® Plus in piglet feed also had a significant effect on feed conversion ratio (P=0,0046). No significant influence of the interaction of these two parameters on the total and daily weight gain, total and daily feed consumption and feed conversion ratio during the experiment was found (P>0.05).
PB  - Univerzitet u Beogradu, Fakultet veterinarske medicine
T1  - Uticaj fitogenog aditiva u kontroli proliferativne enteropatije uz procenu proizvodnih rezultata odlučene prasadi prirodno inficirane bakterijom Lawsonia intracellularis
T1  - The influence of a phytogenic additive in the control of proliferative enteropathy and the assessment of production results in weaned pigs naturally infected with Lawsonia intracellularis
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2268
ER  - 

@phdthesis{
author = "Drašković, Vladimir Lj.",
year = "2021",
abstract = "Uzgoj svinja predstavlja jednu od najvažnijih grana stočarstva. Neadekvatna primena biosigurnosnih mera i loši ambijentalni uslovi, uz prisustvo patogenih prouzrokovača bolesti, mogu onemogućiti uspešnu proizvodnju svinja. U preko 50% slučajeva enterične bolesti su odgovorne za ograničavanje rentabilne proizvodnje. Jedan od važnih uzročnika koji dovodi do enteričnih poremećaja i velikih ekonomskih gubitaka je i Lawsonia intracellularis, obliganta intracelularna Gram negativna bakterija, koja prouzrokuje proliferativnu enteropatiju (PE). „Zlatni standardi“ za identifikaciju L. intracellularis su imunohistohemijska i PCR metoda. Razvojem kvantitativne real-time (qPCR) metode omogućeno je rutinsko otkrivanje subkliničkih formi bolesti, a kvantifikovanje uzročnika je omogućilo da se odredi težina kliničke slike inficiranih svinja. Zbog često pogrešne dijagnostike ove bolesti na farmama, posledično prekomerne upotrebe antibiotika i razvoja antimikrobne rezistencije uzročnika, postoji potreba za pronalaženjem alternativnih rešenja u kontroli PE. Takođe, dobre biosigurnosne mere na farmama, za koje je pokazano da pozitivno utiču na zdravlje i proizvodne rezultate svinja, mogu predstavljati bitan faktor u prevenciji nastanka PE. U skladu sa tim cilj istraživanja ove doktorske disertacije bio je ispitivanje uticaja komercijalnog fitogenog aditiva (Patente Herba® Plus) u kontroli PE kod odlučene prasadi prirodno inficirane bakterijom L. intracellularis, uspostavljanje i optimizacija protokola za molekularnogenetičku identifikaciju bakterije L. intracellularis, kao i kvantifikaciju stepena infekcije kod prasadi putem real-time qPCR i IHC metoda. Dodatno je ispitivan i uticaj različitih biosigurnosnih nivoa i Patente Herba® Plus fitogenog aditiva na proizvodne rezultate i broj izlučenih bakterija L. intracellularis u fecesu prasadi.
Eksperiment je sproveden na četiri različite farme svinja na kojima je određen nivo eksternih, internih i ukupnih biosigurnosnih mera. Na svakoj od farmi (farma BS1, farma BS2, farma BS3 i farma BS4), prasad stara sedam nedelja, ujednačenih telesnih masa, bila je raspoređena u kontrolne grupe koje u hrani nisu dobijale preparat Patente Herba® Plus (K-BS1, K-BS2, K-BS3 i K-BS4) i tretman grupe koje su hrani dobijale preparat Patente Herba® Plus u koncentraciji od 2 kg/t hrane (T-BS1, T-BS2, T-BS3 i T-BS4). Kontrolne i tretman grupe formirane su sa po 72 jedinke, raspoređene u devet bokseva sa po osam prasadi, odnosno 144 prasadi po farmi, ukupno 576 prasadi. Za potrebe histoloških analiza izveden je eksperiment sa individualno gajenom prasadi, po šest u kontrolnoj i tretman grupi. Tokom 28 dana eksperimenta (0., 14. i 28. dan) uzimani su uzorci fecesa za molekularnogenetička ispitivanja, praćeni su proizvodni rezultati prasadi (telesna masa, dnevni i ukupni prorast, dnevna i ukupna konzumacija i konverzija), dok na kraju eksperimenta su uzimani uzorci ileuma za histološke (mikroskopske promene i histomorfometrijski parametari) i imunohistohemijske analize (ekspresija antigena L. intracelullaris).
Na sve četiri farme, u okviru eksternih biosigurnosnih mera, najbolje su bile ocenjene potkategorije koje se odnose na kupovinu životinja i semena i zaposlene i posetioce, dok najniže ocene su dobile biosigurnosne mere koje se odnose na hranu, vodu i opremu. U okviru internih biosigurnosnih mera potkategorije kontrola bolesti i prasilište i period dojenja bile su najbolje ocenjene na farmama, a najlošije su bile ocenjene biosigurnosne mere koje se odnose na čišćenje i dezinfekciju, odgajivalište i mere između odeljaka i korišćenje opreme. Ukupne vrednosti biosigurnosnih mera na sve četiri farme (BS 1 60%, BS 2 64%, BS 3 77% i BS 4 86%) koje su analizirane u ovoj doktorskoj disertaciji bile su više od prosečnih vrednosti biosigurnosnih mera na farmama u Srbiji (58%) i svetu (64%), izuzev farme BS 1 čija ocena ukupnih biosigurnosnih mera je bila niža u poređenju sa prosečnim vrednostima u svetu (60%).
Na farmi BS1 utvrđen je značajno manji broj bakterija L. intracellularis u fecesu prasadi tretman grupe u odnosu na kontrolnu grupu 14. i 28. dana eksperimenta (P=0,0080 i P=0,0015, pojedinačno). Takođe, prasad tretman grupe imala je značajno manji broj bakterija L. intracellularis u fecesu 14. i 28. dana u odnosu na 0. dan eksperimenta (P<0,0001). Na farmi BS 2 uočen je značajan pozitivni efekat preparata Patente Herba® Plus u hrani tretman grupe prasadi na broj bakterija L. intracellularis 14. i 28. dana ogleda u odnosu na kontrolnu grupu (P=0,0470 i P=0,0176, pojedinačno), dok nije uočena značajna razlika u broju bakterija L. intracellularis u fecesu prasadi tretman grupe tokom trajanja eksperimenta (P=0,6174). Na farmama BS 3 i BS 4 nije uočena razlika između kontrolne i tretman grupe prasadi u broju bakterija L. intracellularis za sva tri ispitivana perioda (P>0,05), niti je uočen značajan pad broja bakterija L. intracellularis u fecesu prasadi tretman grupa tokom trajanja ogleda (P>0,05).
Uočena je značajna negativna korelaciona zavisnost (r=-0,664, P<0,01) između broja bakterija L. intracellularis u fecesu prasadi i biosigurnosnih nivoa farmi, dok nije utvrđena značajna korelaciona zavisnost između broja bakterija L. intracellularis, tretmana fitogenim aditivom i dužine trajanja tretmana (P>0,05).
Histološke promene na preparatima ileuma kontrolne i tretman grupe prasadi su deskriptivno analizirane, dok je histomorfometrijskim ispitivanjima pokazano da su u tretman grupi prasadi kripte bile značajno pliće (P=0,0284), a odnos visina resica/dubina kripti značajno veći (P=0,0040) nego u
kontrolnoj grupi. Nije uočen efekat dodavanja fitogenog aditiva u hrani prasadi na visinu resica (P=0,0607), širinu resica (P=0,0728), površinu resica (P=0,7676) i broj peharastih ćelija/100 enterocita (P=0,0575).
Semikvantitativnom analizom, na osnovu imunohistohemijskih nalaza, 33,33% uzoraka ilealnog tkiva tretman grupe prasadi bilo je ocenjeno ocenom 0, a preostalih 66,67% ocenom 1, dok je u kontrolnoj grupi 50% uzoraka bilo ocenjeno ocenom 1, 33,33% ocenom 2, a 16,67% uzoraka ocenom 3.
Primena fitogenog aditiva u hrani nije značajno uticala na telesnu masu prasadi nakon 28 dana eksperimenta na svim ispitivanim farmama (P>0,05). Ukupni i dnevni prirast prasadi tretman grupe bio je značajno veći na farmi BS 2 u poređenju sa kontrolnom grupom (P=0,0349 i P=0,0345, pojedinačno), dok na farmama BS 1, BS 3 i BS 4 razlike u ukupnom i dnevnom prirastu tretman i kontrolne grupe nisu uočene (P>0,05). Najbolji prirast je postigla grupa koja je u hrani dobijala preparat Patente Herba® Plus na farmi BS 4, koji je bio značajno viši u poređenju sa K-BS1, K-BS2 i T-BS2 grupom prasadi (P<0,0001).
Na kraju eksperimenta (28. dan) nije uočena razlika u ukupnoj i dnevnoj konzumaciji hrane između kontrolne i tretman grupe prasadi u okviru svake farme (P>0,05). Značajno bolja konverzija tretman grupe prasadi u odnosu na kontrolnu grupu utvrđena je na farmi BS 1 (P=0,0121), dok na drugim farmama nije uočena razlika u konverziji hrane prasadi kontrolne i tretman grupe (P>0,05).
Dvofaktorijalnom analizom varijanse utvđen je značajan efekat primene preparata Patente Herba® Plus i biosigurnosnih nivoa farmi na ukupan i dnevni prirast prasadi (P<0,05). Biosigurnosni nivoi na farmama su značajno uticali i na ukupnu i dnevnu konzumaciju hrane (P=0,0435 i P=0,0434, pojedinačno), dok je dodavanje Patente Herba® Plus u hrani prasadi imalo i značajan efekat na konverziju (P=0,0046). Nije uočen značajan uticaj interakcije ova dva parametra na ukupni i dnevni prirast, ukupnu i dnevnu konzumaciju i konverziju hrane prasadi tokom ogleda (P>0,05)., Pig breeding is one of the most important branches of animal husbandry. Inadequate application of biosecurity measures and poor environmental conditions, in the presence of pathogens, could disable successful pig production. In over 50% of cases, enteric diseases could effect profitable production. One of the important enteric pathogens associated with large economic losses is Lawsonia intracellularis, an obligate intracellular Gram-negative bacterium, which causes proliferative enteropathy (PE). The “gold standards” for identifying L. intracellularis are immunohistochemical and PCR methods. The development of the quantitative real-time (qPCR) method enabled the routine detection of subclinical forms of the disease, and the quantification of the bacteria gives the possibility to determine the severity of the clinical symptoms in infected pigs. Since this infection is often misdiagnosed on pig farms, this results in excessive use of antibiotics and the ability of bacteria to develop resistance to antimicrobial agents. Therefore, it is necessary to find alternative solutions in PE control. Also, good biosecurity measures on farms, for which it is shown to have positive effect on health and production results of pigs, can be an important factor in the prevention of PE. Accordingly, the aim of this doctoral dissertation was to investigate the effect of commercial phytogenic additive (Patente Herba® Plus) in PE control in weaned piglets naturally infected with L. intracellularis, to establish and optimize protocols for molecular genetic identification of L. intracellularis, as well as to quantify the degree of infection in piglets via real-time qPCR and IHC methods. Additionally, the effect of different biosecurity levels and Patente Herba® Plus phytogenic additive on the production results and the number of excreted L. intracellularis in the faeces of piglets were investigated.
The experiment was conducted on four different pig farms on which the levels of external, internal and total biosecurity measures were determined. On each of the farms (farm BS1, farm BS2, farm BS3 and farm BS4), seven-week-old piglets of uniform body weight were assigned to control groups that did not receive Patente Herba® Plus (K-BS1, K-BS2, K-BS3 and K-BS4) and the treatment groups that received Patente Herba® Plus in their feed at a concentration of 2 kg/t feed (T-BS1, T-BS2, T-BS3 and T-BS4). Control and treatment groups consisted of 72 piglets each, distributed in nine pens with eight piglets each, i.e. 144 piglets per farm, a total of 576 piglets. For histological analyzes, an additional experiment with individually reared piglets was performed, which consistet of six piglets in each, control and treatment group. During the 28 days of the experiment (days 0, 14, and 28) faeces samples
were taken for molecular genetic analysis, the production results of piglets (body weight, daily and total weight gain, daily and total consumption and feed conversion ratio) were monitored, while at the end of the experiment ileum samples were taken for histological (microscopic changes and histomorphometric parameters) and immunohistochemical analyzes (L. intracelullaris antigen expression). On all four farms, within the external biosecurity, the subcategories related to the purchase of animals and semen and personnel and visitors were rated the best, while the biosecurity measures related to the feed, water and equipment supply were rated the lowest. Within the internal biosecurity, the subcategories of disease management and farrowing and suckling period had the highest score on farms, while the lowest score had biosecurity measures related to cleaning and disinfection, nursery unit and measures between compartments and use of equipment. The total biosecurity values of all four examined farms (BS 1 60%, BS 2 64%, BS 3 77% and BS 4 86%) in this doctoral dissertation were higher than the average biosecurity values on farms in Serbia (58%) and in the world (64%), with exception of farm BS 1 where total biosecurity value was lower compared to the average values in the world (60%).
On the farm BS1, a significantly lower number of L. intracellularis was found in the faeces of piglets in the treatment group compared to the control group on days 14 and 28 of the experiment (P=0.0080 and P=0.0015, respectively). Also, the piglets of the treatment group had a significantly lower number of L. intracellularis in the faeces on days 14 and 28 compared to day 0 of the experiment (P<0.0001). On the farm BS 2, a significant positive effect of Patente Herba® Plus in the feed on the number of L. intracellularis in the treatment group of piglets was observed on the 14th and 28th day of the experiment in relation to the control group (P=0.0470 and P=0.0176, respectively), while in the treatment group alone no significant difference was observed in the number of L. intracellularis in the faeces of piglets during the experiment (P=0.6174). On the farm BS 3 and BS 4, there were no significant difference between the control and treatment groups of piglets in the number of L. intracellularis in faeces for all three examined periods (P>0.05), also a significant decrease in the number of L. intracellularis in the faeces of piglets in the treatment groups during the experiment was not observed (P>0.05).
A significant negative correlation was determined (r=-0.664, P<0.01) between the number of L. intracellularis in faeces and on-farm biosecurity levels, while no significant correlation was found between the number of L. intracellularis in faeces, the phytogenic additive treatment and the treatment duration (P>0.05).
Histological changes on ileum preparations of the control and the treatment groups of piglets were descriptively analyzed, while histomorphometric examinations showed that in the treatment group the crypts were significantly shallower (P=0.0284) and the villus height/crypt depth ratio significantly higher (P=0.0040) than in the control group. No effect of the addition of phytogenic additive was observed in piglet feed on villus height (P=0.0607), villus width (P=0.0728), villus surface area (P=0.7676) and the number of goblet cells/100 enterocytes (P=0.0575).
Using semiquantitative analysis, based on immunohistochemical findings, 33.33% of ileal tissue samples of the treatment group piglets were rated with a grade 0, and the remaining 66.67% with a grade 1, while in the control group 50% of samples were rated with a grade 1, 33.33% with grade 2, and 16.67% of samples with grade 3.
The use of phytogenic additive in feed did not significantly affect the body weight of piglets after 28 days of the experiment on all tested farms (P>0.05). The total and daily weight gain of piglets in the treatment group was significantly higher compared to the control group on the farm BS 2 (P=0.0349 and P=0.0345, respectively), while on farms BS 1, BS 3 and BS 4 the differences in total and daily weight gain in treatment and control groups of piglets were not observed (P>0.05). The best weight gain was achieved in the group that received the preparation Patente Herba® Plus in feed on farm BS 4, which was significantly higher compared to K-BS1, K-BS2 and T-BS2 group of piglets (P<0.0001).
At the end of the experiment (day 28), no differences in total and daily feed consumption were observed between the control and treatment groups of piglets within each farm (P>0.05). Significantly better feed conversion ratio of piglet in the treatment group compared to the control was found on farm BS 1 (P=0.0121), while on other farms no differences were observed in the feed conversion ratio of piglets in control and treatment groups (P>0.05).
Two-way analysis of variance revealed a significant effect of the application of Patente Herba® Plus and biosecurity levels of farms on the total and daily weight gain of piglets (P<0.05). Biosecurity levels on farms significantly affected both total and daily feed consumption (P=0.0435 and P=0.0434, respectively), while the addition of Patente Herba® Plus in piglet feed also had a significant effect on feed conversion ratio (P=0,0046). No significant influence of the interaction of these two parameters on the total and daily weight gain, total and daily feed consumption and feed conversion ratio during the experiment was found (P>0.05).",
publisher = "Univerzitet u Beogradu, Fakultet veterinarske medicine",
title = "Uticaj fitogenog aditiva u kontroli proliferativne enteropatije uz procenu proizvodnih rezultata odlučene prasadi prirodno inficirane bakterijom Lawsonia intracellularis, The influence of a phytogenic additive in the control of proliferative enteropathy and the assessment of production results in weaned pigs naturally infected with Lawsonia intracellularis",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2268"
}

Drašković, V. Lj.. (2021). Uticaj fitogenog aditiva u kontroli proliferativne enteropatije uz procenu proizvodnih rezultata odlučene prasadi prirodno inficirane bakterijom Lawsonia intracellularis. 
Univerzitet u Beogradu, Fakultet veterinarske medicine..
https://hdl.handle.net/21.15107/rcub_veterinar_2268

Drašković VL. Uticaj fitogenog aditiva u kontroli proliferativne enteropatije uz procenu proizvodnih rezultata odlučene prasadi prirodno inficirane bakterijom Lawsonia intracellularis. 2021;.
https://hdl.handle.net/21.15107/rcub_veterinar_2268 .

Drašković, Vladimir Lj., "Uticaj fitogenog aditiva u kontroli proliferativne enteropatije uz procenu proizvodnih rezultata odlučene prasadi prirodno inficirane bakterijom Lawsonia intracellularis" (2021),
https://hdl.handle.net/21.15107/rcub_veterinar_2268 .

TY  - GEN
AU  - Stanišić, Ljubodrag
AU  - Aleksić, Jelena M.
AU  - Ristanić, Marko
AU  - Stevanović, Jevrosima
AU  - Dimitrijević, Vladimir
AU  - Stanimirović, Zoran
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3200
AB  - Balkanski magarac predstavlja dominantnu rasu magaraca i genetički resurs
Republike Srbije. Nalazi se na listi autohtonih rasa domaćih životinja i ugroženih
autohtonih rasa u Republici Srbiji. Balkanski magarac, na osnovu glavnih
morfometrijskih parametara, spada u magarce srednje veličine, a boja dlake
je uglavnom siva do različitih nijansi braon boje sa jeguljastom prugom duž
leđa i karakterističnim krstom na grebenu. Međutim, lokalni uzgajivači iz Srbije
i dalje poseduju nekoliko morfološki većih jedinki svetlije boje dlake, nazvane
banatski magarac Za njih smatraju da potiču od rasa španskih magaraca, a
po verovanju se pretpostavlja da su prebačeni u region Banata od strane habsburške
kraljice Marije Terezije u 18. veku. Na osnovu komparativne analize, 18
morfoloških karakteristika između banatskih magaraca (7 jedinki), banatskog
magarca (53 jedinke) i njihovih hibrida (8 jedinki), uočene su telesne mere koje
statistički odvajaju populacije banatskih i balkanskih magaraca a to su: visina
kuka, širina sapi, dužina trupa i dubina grudi. Na osnovu podataka nuklearnih
mikrosatelita, genetski udaljen banatski magarac nije ozbiljno pogođen gubitkom
genetičkog diverziteta i inbridingom. Geneološke analize i upoređivanje
prethodno objavljenih haplotipova mtDNK rasa magaraca iz Srbije, sa drugim haplotipovima drevnih i današnjih rasa magaraca, ukazuju na povezanost banatskog
magarca sa somalijskim magarcem i kompleksniju istoriju nastanka
Clade 2.
PB  - Beograd : Srpsko veterinarsko društvo
T2  - Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021
T1  - Molekularno-genetičke i fenotipske karakteristike balkanskog i banatskog magarca
SP  - 365
EP  - 374
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3200
ER  - 

@misc{
author = "Stanišić, Ljubodrag and Aleksić, Jelena M. and Ristanić, Marko and Stevanović, Jevrosima and Dimitrijević, Vladimir and Stanimirović, Zoran",
year = "2021",
abstract = "Balkanski magarac predstavlja dominantnu rasu magaraca i genetički resurs
Republike Srbije. Nalazi se na listi autohtonih rasa domaćih životinja i ugroženih
autohtonih rasa u Republici Srbiji. Balkanski magarac, na osnovu glavnih
morfometrijskih parametara, spada u magarce srednje veličine, a boja dlake
je uglavnom siva do različitih nijansi braon boje sa jeguljastom prugom duž
leđa i karakterističnim krstom na grebenu. Međutim, lokalni uzgajivači iz Srbije
i dalje poseduju nekoliko morfološki većih jedinki svetlije boje dlake, nazvane
banatski magarac Za njih smatraju da potiču od rasa španskih magaraca, a
po verovanju se pretpostavlja da su prebačeni u region Banata od strane habsburške
kraljice Marije Terezije u 18. veku. Na osnovu komparativne analize, 18
morfoloških karakteristika između banatskih magaraca (7 jedinki), banatskog
magarca (53 jedinke) i njihovih hibrida (8 jedinki), uočene su telesne mere koje
statistički odvajaju populacije banatskih i balkanskih magaraca a to su: visina
kuka, širina sapi, dužina trupa i dubina grudi. Na osnovu podataka nuklearnih
mikrosatelita, genetski udaljen banatski magarac nije ozbiljno pogođen gubitkom
genetičkog diverziteta i inbridingom. Geneološke analize i upoređivanje
prethodno objavljenih haplotipova mtDNK rasa magaraca iz Srbije, sa drugim haplotipovima drevnih i današnjih rasa magaraca, ukazuju na povezanost banatskog
magarca sa somalijskim magarcem i kompleksniju istoriju nastanka
Clade 2.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021",
title = "Molekularno-genetičke i fenotipske karakteristike balkanskog i banatskog magarca",
pages = "365-374",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3200"
}

Stanišić, L., Aleksić, J. M., Ristanić, M., Stevanović, J., Dimitrijević, V.,& Stanimirović, Z.. (2021). Molekularno-genetičke i fenotipske karakteristike balkanskog i banatskog magarca. in Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021
Beograd : Srpsko veterinarsko društvo., 365-374.
https://hdl.handle.net/21.15107/rcub_veterinar_3200

Stanišić L, Aleksić JM, Ristanić M, Stevanović J, Dimitrijević V, Stanimirović Z. Molekularno-genetičke i fenotipske karakteristike balkanskog i banatskog magarca. in Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021. 2021;:365-374.
https://hdl.handle.net/21.15107/rcub_veterinar_3200 .

Stanišić, Ljubodrag, Aleksić, Jelena M., Ristanić, Marko, Stevanović, Jevrosima, Dimitrijević, Vladimir, Stanimirović, Zoran, "Molekularno-genetičke i fenotipske karakteristike balkanskog i banatskog magarca" in Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021 (2021):365-374,
https://hdl.handle.net/21.15107/rcub_veterinar_3200 .

TY  - JOUR
AU  - Došenović, Milan
AU  - Radaković, Milena
AU  - Vučićević, Miloš
AU  - Vejnović, Branislav
AU  - Vasiljević, Maja
AU  - Marinković, Darko
AU  - Stanimirović, Zoran
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2059
AB  - The aim of this study was to assess how red-eared sliders (Trachemys scripta elegans) respond to anaesthesia itself and coelioscopy. For that purpose, the turtles were anaesthetised with ketamine–medetomidine or propofol, and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and the level of malondialdehyde (MDA) were determined by spectrophotometry. The possible genotoxic effects of the anaesthetic agents were estimated by comet assay. A total of 24 turtles were included in this study. The animals were divided into four groups according to the anaesthetic protocol and according to whether endoscopy would be performed. Significantly decreased activities of CAT were found only in the propofol group and in turtles undergoing coelioscopy. Both anaesthetic protocols induced significantly increased MDA levels, while no differences were observed after the intervention. A significant increase in GST activity was detected in turtles after both anaesthetic protocols, but after coelioscopy significant changes in GST activity were found only in the propofol group. However, no differences in SOD activity and no DNA damages were detected in either group. These findings suggest that ketamine–medetomidine may be more suitable anaesthetic agents in red-eared sliders than propofol.
PB  - AKJournals
T2  - Acta Veterinaria Hungarica
T1  - Evaluation of the effects of two anaesthetic protocols on oxidative status and DNA damage in red-eared sliders (Trachemys scripta elegans) undergoing endoscopic coeliotomy
VL  - 68
IS  - 4
SP  - 337
EP  - 344
DO  - 10.1556/004.2020.00058
ER  - 

@article{
author = "Došenović, Milan and Radaković, Milena and Vučićević, Miloš and Vejnović, Branislav and Vasiljević, Maja and Marinković, Darko and Stanimirović, Zoran",
year = "2021",
abstract = "The aim of this study was to assess how red-eared sliders (Trachemys scripta elegans) respond to anaesthesia itself and coelioscopy. For that purpose, the turtles were anaesthetised with ketamine–medetomidine or propofol, and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and the level of malondialdehyde (MDA) were determined by spectrophotometry. The possible genotoxic effects of the anaesthetic agents were estimated by comet assay. A total of 24 turtles were included in this study. The animals were divided into four groups according to the anaesthetic protocol and according to whether endoscopy would be performed. Significantly decreased activities of CAT were found only in the propofol group and in turtles undergoing coelioscopy. Both anaesthetic protocols induced significantly increased MDA levels, while no differences were observed after the intervention. A significant increase in GST activity was detected in turtles after both anaesthetic protocols, but after coelioscopy significant changes in GST activity were found only in the propofol group. However, no differences in SOD activity and no DNA damages were detected in either group. These findings suggest that ketamine–medetomidine may be more suitable anaesthetic agents in red-eared sliders than propofol.",
publisher = "AKJournals",
journal = "Acta Veterinaria Hungarica",
title = "Evaluation of the effects of two anaesthetic protocols on oxidative status and DNA damage in red-eared sliders (Trachemys scripta elegans) undergoing endoscopic coeliotomy",
volume = "68",
number = "4",
pages = "337-344",
doi = "10.1556/004.2020.00058"
}

Došenović, M., Radaković, M., Vučićević, M., Vejnović, B., Vasiljević, M., Marinković, D.,& Stanimirović, Z.. (2021). Evaluation of the effects of two anaesthetic protocols on oxidative status and DNA damage in red-eared sliders (Trachemys scripta elegans) undergoing endoscopic coeliotomy. in Acta Veterinaria Hungarica
AKJournals., 68(4), 337-344.
https://doi.org/10.1556/004.2020.00058

Došenović M, Radaković M, Vučićević M, Vejnović B, Vasiljević M, Marinković D, Stanimirović Z. Evaluation of the effects of two anaesthetic protocols on oxidative status and DNA damage in red-eared sliders (Trachemys scripta elegans) undergoing endoscopic coeliotomy. in Acta Veterinaria Hungarica. 2021;68(4):337-344.
doi:10.1556/004.2020.00058 .

Došenović, Milan, Radaković, Milena, Vučićević, Miloš, Vejnović, Branislav, Vasiljević, Maja, Marinković, Darko, Stanimirović, Zoran, "Evaluation of the effects of two anaesthetic protocols on oxidative status and DNA damage in red-eared sliders (Trachemys scripta elegans) undergoing endoscopic coeliotomy" in Acta Veterinaria Hungarica, 68, no. 4 (2021):337-344,
https://doi.org/10.1556/004.2020.00058 . .

TY  - JOUR
AU  - Davitkov, Dajana
AU  - Marinković, Darko
AU  - Davitkov, Darko
AU  - Nešić, Vladimir
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1867
AB  - Congenital spine deformation caused by vertebral malformation is reported in different brachycephalic dog breeds, but also in other large dog breeds. The most severe clinical signs  are  due  to  spinal  cord  compression.  Depending  on  localisation  of   the  lesion,  clinical signs can manifest differently. A five-month-old pug was brought for autopsy to the Department of  Forensic Veterinary Medicine, Faculty of  Veterinary Medicine, University of Belgrade. The first clinical signs, including general weakness and difficulty to jump appeared when he was three and a half  months old. They became more severe until  the  dog  was  four  months  old.  Radiography  and  myelography  showed  severe  kyphosis and spinal cord compression in the T8-T9 region. After euthanasia, an autopsy was  performed.  Pathomorphological  examination  showed  severe  deformation  of   the  spine, kyphosis and a wedge-shaped T8 vertebra. Although, literature data indicates that clinical signs can be latent until five or six months of age, this case shows that even in young puppies, three to four months old, serious clinical signs can be present. This is of  great clinical importance, not just for veterinarians, but also for brachycephalic dog owners and breeders.
AB  - Kongenitalne deformacije kičme uzrokovane malformacijama na vertebrama javljaju se kod različitih brahicefaličnih rasa, ali se mogu javiti i kod velikih rasa pasa. Najteži klinički simptomi se javljaju usled kompresije kičmene moždine. U zavisnosti od lokalizacije lezije, simptomi se mogu različito manifestovati. Leš mopsa starosti pet meseci donešen je zbog obdukcije na Katedru za sudsku veterinarsku medicinu, Fakulteta veterinarske medicine Univerziteta u Beogradu. Prvi klinički simptomi, u vidu opšte slabosti i poteškoća pri skakanju, javili su se kada je štene bilo staro tri i po meseca. Simptomi su u četvrtom mesecu postali mnogo ozbiljniji. Nativno rendgensko i mijelografsko snimanje pokazalo je uznapredovali stepen kifoze i kompresije kičmene moždine u T8-T9 regionu kičme. Nakon eutanazije, pas je obdukovan. Patomorfološki nalaz ukazao je na ozbiljnu deformaciju kičme, izraženu kifozu i klinast oblik T8 pršljena. Iako literaturni podaci ukazuju da klinički simptomi mogu biti nezapaženi sve do pet ili šest meseci starosti, ovaj slučaj pokazuje da čak kod mlade štenadi, starosti tri do četiri meseca, mogu biti prisutni ozbiljni simptomi. Ova činjenica je od velikog značaja, ne samo za veterinare, već i za odgajivače i vlasnike brahicefaličnih rasa pasa.
PB  - Fakultet veterinarske medicine
T2  - Veterinarski Glasnik
T2  - Veterinarski Glasnik
T1  - Congenital thoracic kyphosis caused by hemivertebra in a Pug puppy
T1  - Kongenitalna kifoza u torakalnom delu kičme  usled prisustva hemivertebre kod šteneta mopsa
VL  - 74
IS  - 1
SP  - 92
SP  - 92
EP  - 98
EP  - 98
DO  - 10.2298/VETGL190115007D
ER  - 

@article{
author = "Davitkov, Dajana and Marinković, Darko and Davitkov, Darko and Nešić, Vladimir",
year = "2020",
abstract = "Congenital spine deformation caused by vertebral malformation is reported in different brachycephalic dog breeds, but also in other large dog breeds. The most severe clinical signs  are  due  to  spinal  cord  compression.  Depending  on  localisation  of   the  lesion,  clinical signs can manifest differently. A five-month-old pug was brought for autopsy to the Department of  Forensic Veterinary Medicine, Faculty of  Veterinary Medicine, University of Belgrade. The first clinical signs, including general weakness and difficulty to jump appeared when he was three and a half  months old. They became more severe until  the  dog  was  four  months  old.  Radiography  and  myelography  showed  severe  kyphosis and spinal cord compression in the T8-T9 region. After euthanasia, an autopsy was  performed.  Pathomorphological  examination  showed  severe  deformation  of   the  spine, kyphosis and a wedge-shaped T8 vertebra. Although, literature data indicates that clinical signs can be latent until five or six months of age, this case shows that even in young puppies, three to four months old, serious clinical signs can be present. This is of  great clinical importance, not just for veterinarians, but also for brachycephalic dog owners and breeders., Kongenitalne deformacije kičme uzrokovane malformacijama na vertebrama javljaju se kod različitih brahicefaličnih rasa, ali se mogu javiti i kod velikih rasa pasa. Najteži klinički simptomi se javljaju usled kompresije kičmene moždine. U zavisnosti od lokalizacije lezije, simptomi se mogu različito manifestovati. Leš mopsa starosti pet meseci donešen je zbog obdukcije na Katedru za sudsku veterinarsku medicinu, Fakulteta veterinarske medicine Univerziteta u Beogradu. Prvi klinički simptomi, u vidu opšte slabosti i poteškoća pri skakanju, javili su se kada je štene bilo staro tri i po meseca. Simptomi su u četvrtom mesecu postali mnogo ozbiljniji. Nativno rendgensko i mijelografsko snimanje pokazalo je uznapredovali stepen kifoze i kompresije kičmene moždine u T8-T9 regionu kičme. Nakon eutanazije, pas je obdukovan. Patomorfološki nalaz ukazao je na ozbiljnu deformaciju kičme, izraženu kifozu i klinast oblik T8 pršljena. Iako literaturni podaci ukazuju da klinički simptomi mogu biti nezapaženi sve do pet ili šest meseci starosti, ovaj slučaj pokazuje da čak kod mlade štenadi, starosti tri do četiri meseca, mogu biti prisutni ozbiljni simptomi. Ova činjenica je od velikog značaja, ne samo za veterinare, već i za odgajivače i vlasnike brahicefaličnih rasa pasa.",
publisher = "Fakultet veterinarske medicine",
journal = "Veterinarski Glasnik, Veterinarski Glasnik",
title = "Congenital thoracic kyphosis caused by hemivertebra in a Pug puppy, Kongenitalna kifoza u torakalnom delu kičme  usled prisustva hemivertebre kod šteneta mopsa",
volume = "74",
number = "1",
pages = "92-92-98-98",
doi = "10.2298/VETGL190115007D"
}

Davitkov, D., Marinković, D., Davitkov, D.,& Nešić, V.. (2020). Congenital thoracic kyphosis caused by hemivertebra in a Pug puppy. in Veterinarski Glasnik
Fakultet veterinarske medicine., 74(1), 92-98.
https://doi.org/10.2298/VETGL190115007D

Davitkov D, Marinković D, Davitkov D, Nešić V. Congenital thoracic kyphosis caused by hemivertebra in a Pug puppy. in Veterinarski Glasnik. 2020;74(1):92-98.
doi:10.2298/VETGL190115007D .

Davitkov, Dajana, Marinković, Darko, Davitkov, Darko, Nešić, Vladimir, "Congenital thoracic kyphosis caused by hemivertebra in a Pug puppy" in Veterinarski Glasnik, 74, no. 1 (2020):92-98,
https://doi.org/10.2298/VETGL190115007D . .

TY  - CONF
AU  - Maletić, Milan
AU  - Pavlović, Miloš
AU  - Đurić, Miloje
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3701
AB  - Toplotni stres ima negativan uticaj na plodnost mlečnih krava koja se uzgajaju
u umerenim i toplim predelima, mada su stepen i trajanje tog uticaja veći u
toplijim regionima sveta. Reproduktivni poremećaji koji su u vezi sa smanjenom
plodnošću zbog toplotnog stresa su: slabije izraženi znaci estrusa, poremećaj folikularne
i oocitne funkcije i povećan embrionalni mortalitet. Kao posledica toplotnog
stresa u kasnoj gestaciji, dolazi do smanjenja fetalnog rasta i pada mlečnosti
u narednoj laktaciji. Ovaj rad govori o uticaju toplotnog stresa na plodnost mlečnih
krava i razmatra mere koje se mogu koristiti u cilju lakšeg prevazilaženja negativnih
efekata toplotnog stresa.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020
T1  - Toplotni stres kao faktor rizika u reprodukciji visoko mlečnih krava
SP  - 97
EP  - 104
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3701
ER  - 

@conference{
author = "Maletić, Milan and Pavlović, Miloš and Đurić, Miloje",
year = "2020",
abstract = "Toplotni stres ima negativan uticaj na plodnost mlečnih krava koja se uzgajaju
u umerenim i toplim predelima, mada su stepen i trajanje tog uticaja veći u
toplijim regionima sveta. Reproduktivni poremećaji koji su u vezi sa smanjenom
plodnošću zbog toplotnog stresa su: slabije izraženi znaci estrusa, poremećaj folikularne
i oocitne funkcije i povećan embrionalni mortalitet. Kao posledica toplotnog
stresa u kasnoj gestaciji, dolazi do smanjenja fetalnog rasta i pada mlečnosti
u narednoj laktaciji. Ovaj rad govori o uticaju toplotnog stresa na plodnost mlečnih
krava i razmatra mere koje se mogu koristiti u cilju lakšeg prevazilaženja negativnih
efekata toplotnog stresa.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020",
title = "Toplotni stres kao faktor rizika u reprodukciji visoko mlečnih krava",
pages = "97-104",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3701"
}

Maletić, M., Pavlović, M.,& Đurić, M.. (2020). Toplotni stres kao faktor rizika u reprodukciji visoko mlečnih krava. in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 97-104.
https://hdl.handle.net/21.15107/rcub_veterinar_3701

Maletić M, Pavlović M, Đurić M. Toplotni stres kao faktor rizika u reprodukciji visoko mlečnih krava. in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020. 2020;:97-104.
https://hdl.handle.net/21.15107/rcub_veterinar_3701 .

Maletić, Milan, Pavlović, Miloš, Đurić, Miloje, "Toplotni stres kao faktor rizika u reprodukciji visoko mlečnih krava" in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020 (2020):97-104,
https://hdl.handle.net/21.15107/rcub_veterinar_3701 .

TY  - JOUR
AU  - Dolasević, Slobodan
AU  - Stevanović, Jevrosima
AU  - Aleksić, Nevenka
AU  - Glavinić, Uroš
AU  - Deletić, Nebojša
AU  - Mladenović, Mica
AU  - Stanimirović, Zoran
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1801
AB  - The influence of various diets on the quality of Apis mellifera queens was assessed. Colonies intended for queen cell production were assigned to four groups fed on (1) sugar-only, (2) mix of sugar, honey and fresh pollen, (3) sugar and pollen substitute (FeedBee?), and (4) natural sources. In addition, a fifth group had queen cells obtained naturally, by swarming. Sugar-only diet exerted a significant (p?<?0.05) stimulating effect on the acceptance of queen cells and the weight of newly emerged queens, not affecting mated queens. Among mated queens those raised by bees fed on the mix of sugar, honey and fresh pollen had significantly (p?<?0.05) larger numbers of ovarioles in comparison with all the others. Their weight was significantly higher than that of the queens from groups given FeedBee(?) and swarming queens. Compared to the latter, they had significantly wider spermatheca. Given the parameters monitored, FeedBee(?) proved not to be advantageous for queens.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Apicultural Research
T1  - The effect of diet types on some quality characteristics of artificially reared Apis mellifera queens
VL  - 59
IS  - 1
SP  - 115
EP  - 123
DO  - 10.1080/00218839.2019.1673965
ER  - 

@article{
author = "Dolasević, Slobodan and Stevanović, Jevrosima and Aleksić, Nevenka and Glavinić, Uroš and Deletić, Nebojša and Mladenović, Mica and Stanimirović, Zoran",
year = "2020",
abstract = "The influence of various diets on the quality of Apis mellifera queens was assessed. Colonies intended for queen cell production were assigned to four groups fed on (1) sugar-only, (2) mix of sugar, honey and fresh pollen, (3) sugar and pollen substitute (FeedBee?), and (4) natural sources. In addition, a fifth group had queen cells obtained naturally, by swarming. Sugar-only diet exerted a significant (p?<?0.05) stimulating effect on the acceptance of queen cells and the weight of newly emerged queens, not affecting mated queens. Among mated queens those raised by bees fed on the mix of sugar, honey and fresh pollen had significantly (p?<?0.05) larger numbers of ovarioles in comparison with all the others. Their weight was significantly higher than that of the queens from groups given FeedBee(?) and swarming queens. Compared to the latter, they had significantly wider spermatheca. Given the parameters monitored, FeedBee(?) proved not to be advantageous for queens.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Apicultural Research",
title = "The effect of diet types on some quality characteristics of artificially reared Apis mellifera queens",
volume = "59",
number = "1",
pages = "115-123",
doi = "10.1080/00218839.2019.1673965"
}

Dolasević, S., Stevanović, J., Aleksić, N., Glavinić, U., Deletić, N., Mladenović, M.,& Stanimirović, Z.. (2020). The effect of diet types on some quality characteristics of artificially reared Apis mellifera queens. in Journal of Apicultural Research
Taylor & Francis Ltd, Abingdon., 59(1), 115-123.
https://doi.org/10.1080/00218839.2019.1673965

Dolasević S, Stevanović J, Aleksić N, Glavinić U, Deletić N, Mladenović M, Stanimirović Z. The effect of diet types on some quality characteristics of artificially reared Apis mellifera queens. in Journal of Apicultural Research. 2020;59(1):115-123.
doi:10.1080/00218839.2019.1673965 .

Dolasević, Slobodan, Stevanović, Jevrosima, Aleksić, Nevenka, Glavinić, Uroš, Deletić, Nebojša, Mladenović, Mica, Stanimirović, Zoran, "The effect of diet types on some quality characteristics of artificially reared Apis mellifera queens" in Journal of Apicultural Research, 59, no. 1 (2020):115-123,
https://doi.org/10.1080/00218839.2019.1673965 . .

TY  - JOUR
AU  - Tarić, Elmin
AU  - Glavinić, Uroš
AU  - Vejnović, Branislav
AU  - Stanojković, Aleksandar
AU  - Aleksić, Nevenka
AU  - Dimitrijević, Vladimir
AU  - Stanimirović, Zoran
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1838
AB  - Commercially and traditionally managed bees were compared for oxidative stress (superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and malondialdehyde (MDA)), the prevalence of parasites (<i>Lotmaria passim</i>, <i>Crithidia mellificae</i> and <i>Nosema ceranae/apis</i>) and social immunity (glucose oxidase gene expression). The research was conducted on Pester plateau (Serbia—the Balkan Peninsula), on seemingly healthy colonies. Significant differences in CAT, GST and SOD activities (<i>p</i> < 0.01), and MDA concentrations (<i>p</i> < 0.002) were detected between commercial and traditional colonies. In the former, the prevalence of both <i>L. passim</i> and <i>N. ceranae</i> was significantly (<i>p</i> < 0.05 and <i>p</i> < 0.01, respectively) higher. For the first time, <i>L. passim</i> was detected in honey bee brood. In commercial colonies, the prevalence of <i>L. passim</i> was significantly (<i>p</i> < 0.01) lower in brood than in adult bees, whilst in traditionally kept colonies the prevalence in adult bees and brood did not differ significantly. In commercially kept colonies, the GOX gene expression level was significantly (<i>p</i> < 0.01) higher, which probably results from their increased need to strengthen their social immunity. Commercially kept colonies were under higher oxidative stress, had higher parasite burdens and higher GOX gene transcript levels. It may be assumed that anthropogenic influence contributed to these differences, but further investigations are necessary to confirm that.
PB  - MDPI
T2  - Insects
T1  - Oxidative Stress, Endoparasite Prevalence and Social Immunity in Bee Colonies Kept Traditionally vs. Those Kept for Commercial Purposes
VL  - 11
IS  - 266
SP  - 266
DO  - 10.3390/insects11050266
ER  - 

@article{
author = "Tarić, Elmin and Glavinić, Uroš and Vejnović, Branislav and Stanojković, Aleksandar and Aleksić, Nevenka and Dimitrijević, Vladimir and Stanimirović, Zoran",
year = "2020",
abstract = "Commercially and traditionally managed bees were compared for oxidative stress (superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and malondialdehyde (MDA)), the prevalence of parasites (<i>Lotmaria passim</i>, <i>Crithidia mellificae</i> and <i>Nosema ceranae/apis</i>) and social immunity (glucose oxidase gene expression). The research was conducted on Pester plateau (Serbia—the Balkan Peninsula), on seemingly healthy colonies. Significant differences in CAT, GST and SOD activities (<i>p</i> < 0.01), and MDA concentrations (<i>p</i> < 0.002) were detected between commercial and traditional colonies. In the former, the prevalence of both <i>L. passim</i> and <i>N. ceranae</i> was significantly (<i>p</i> < 0.05 and <i>p</i> < 0.01, respectively) higher. For the first time, <i>L. passim</i> was detected in honey bee brood. In commercial colonies, the prevalence of <i>L. passim</i> was significantly (<i>p</i> < 0.01) lower in brood than in adult bees, whilst in traditionally kept colonies the prevalence in adult bees and brood did not differ significantly. In commercially kept colonies, the GOX gene expression level was significantly (<i>p</i> < 0.01) higher, which probably results from their increased need to strengthen their social immunity. Commercially kept colonies were under higher oxidative stress, had higher parasite burdens and higher GOX gene transcript levels. It may be assumed that anthropogenic influence contributed to these differences, but further investigations are necessary to confirm that.",
publisher = "MDPI",
journal = "Insects",
title = "Oxidative Stress, Endoparasite Prevalence and Social Immunity in Bee Colonies Kept Traditionally vs. Those Kept for Commercial Purposes",
volume = "11",
number = "266",
pages = "266",
doi = "10.3390/insects11050266"
}

Tarić, E., Glavinić, U., Vejnović, B., Stanojković, A., Aleksić, N., Dimitrijević, V.,& Stanimirović, Z.. (2020). Oxidative Stress, Endoparasite Prevalence and Social Immunity in Bee Colonies Kept Traditionally vs. Those Kept for Commercial Purposes. in Insects
MDPI., 11(266), 266.
https://doi.org/10.3390/insects11050266

Tarić E, Glavinić U, Vejnović B, Stanojković A, Aleksić N, Dimitrijević V, Stanimirović Z. Oxidative Stress, Endoparasite Prevalence and Social Immunity in Bee Colonies Kept Traditionally vs. Those Kept for Commercial Purposes. in Insects. 2020;11(266):266.
doi:10.3390/insects11050266 .

Tarić, Elmin, Glavinić, Uroš, Vejnović, Branislav, Stanojković, Aleksandar, Aleksić, Nevenka, Dimitrijević, Vladimir, Stanimirović, Zoran, "Oxidative Stress, Endoparasite Prevalence and Social Immunity in Bee Colonies Kept Traditionally vs. Those Kept for Commercial Purposes" in Insects, 11, no. 266 (2020):266,
https://doi.org/10.3390/insects11050266 . .

TY  - JOUR
AU  - Zikic, Biljana
AU  - Aleksić, Nevenka
AU  - Ristanić, Marko
AU  - Glavinić, Uroš
AU  - Vejnović, Branislav
AU  - Krnjaić, Igor
AU  - Stanimirović, Zoran
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1864
AB  - Apart from the efficiency of coumaphos against Varroa mites, its impact on the oxidative status and survival of the honey bee (Apis mellifera) was assessed. The research was conducted on hives from the same apiary, equalised regarding the number of bees, brood area and food storage. Based on Varroa infestation the hives were allotted to two groups: Non-infested (N) and infested (I). Both groups were either treated (T)-NT and IT, or untreated (U)-NU and IU. The treatment of infested bees was controlled with a follow-up treatment with amitraz. The efficiency of coumaphos was 96-97%. This organophosphate had a negligible effect on bee survival, but it significantly affected their oxidative status: Superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) activities, and the concentrations of malonyl dialdehyde (MDA). Coumaphos significantly (pË 0.0001) decreased SOD activity in non-infested bees, but increased it in those infested. By contrast, both CAT and GST activities, as well as MDA concentrations significantly increased (from pË 0.05 to pË 0.0001) after treatment in all groups, with the exception of IT, where it declined. Coumaphos in non-infested hives caused oxidative stress per se, not unlike varroa in infested colonies. However, in infested colonies it decreased oxidative stress, owing to its efficacy against Varroa mites and contributed to the recovery of bee colonies. In spite of its certain downsides, coumaphos remains an effective anti-varroa substance, but should be used with precaution, not to add to the effects of environmental factors which may cause red-ox misbalance.
AB  - Procenjivana je efikasnost kumafosa protiv varoe, kao i njegov uticaj na oksidativni status i preživljavanje medonosne pčele (Apis mellifera). Ispitivanje je obavljeno na košnicama iz istog pčelinjaka, ujednačenim po broju pčela, površini legla i količini uskladištene hrane. Na osnovu infestacije varoom, košnice su podeljene u dve grupe: neinfestirane (N) i infestirane (I). Obe grupe su bile ili tretirane (T) – NT i IT, ili netretirane (U) – NU i IU. Tretman infestiranih društava je kontrolisan naknadnom primenom amitraza. Efikasnost kumafosa iznosila je 96-97%. Ovaj organofosfat imao Zikic et al.: Anti-varroa efficiency of coumaphos and its influence on oxidative stress and survival of honey bees 373 je zanemarljiv efekat na preživljavanje pčela, ali je značajno uticao na njihov oksidativni status: aktivnosti superoksid-dismutaze (SOD), katalaze (CAT) i glutation Stransferaze (GST), i koncentracije malonil-dialdehida (MDA). Kumafos je značajno (p˂0,0001) smanjio aktivnost SOD kod neinfestiranih pčela, ali ju je povećao kod infestiranih. Za razliku od toga, aktivnosti CAT i GST, kao i koncentracije MDA značajno su se povećale (od p˂0,05 do p˂0,0001) posle tretmana u svim grupama, sa izuzetkom IT, u kojoj su opale. Kumafos je sam po sebi u neinfestiranim društvima prouzrokovao oksidativni stres kod pčela, slično kao Varroa u infestiranim. Međutim, u infestiranim košnicama on je smanjio oksidativni stress zahvaljujući efikasnosti protiv varoe, čime je doprineo oporavku društava. Uprkos određenim nedostacima, kumafos ostaje efikasna supstanca u borbi protiv varoe, ali ga treba koristiti sa oprezom, da bi se izbeglo dodatno opterećenje pčela prouzrokovano faktorima sredine koji mogu da izazovu red-oks neravnotežu.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Anti-Varroa Efficiency of Coumaphos and Its Influence on Oxidative Stress and Survival of Honey Bees
T1  - Efikasnost kumafosa protiv varoe i njegov uticaj na oksidativni stres i preživljavanje medonosne pčele
VL  - 70
IS  - 3
SP  - 355
EP  - 373
DO  - 10.2478/acve-2020-0027
ER  - 

@article{
author = "Zikic, Biljana and Aleksić, Nevenka and Ristanić, Marko and Glavinić, Uroš and Vejnović, Branislav and Krnjaić, Igor and Stanimirović, Zoran",
year = "2020",
abstract = "Apart from the efficiency of coumaphos against Varroa mites, its impact on the oxidative status and survival of the honey bee (Apis mellifera) was assessed. The research was conducted on hives from the same apiary, equalised regarding the number of bees, brood area and food storage. Based on Varroa infestation the hives were allotted to two groups: Non-infested (N) and infested (I). Both groups were either treated (T)-NT and IT, or untreated (U)-NU and IU. The treatment of infested bees was controlled with a follow-up treatment with amitraz. The efficiency of coumaphos was 96-97%. This organophosphate had a negligible effect on bee survival, but it significantly affected their oxidative status: Superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) activities, and the concentrations of malonyl dialdehyde (MDA). Coumaphos significantly (pË 0.0001) decreased SOD activity in non-infested bees, but increased it in those infested. By contrast, both CAT and GST activities, as well as MDA concentrations significantly increased (from pË 0.05 to pË 0.0001) after treatment in all groups, with the exception of IT, where it declined. Coumaphos in non-infested hives caused oxidative stress per se, not unlike varroa in infested colonies. However, in infested colonies it decreased oxidative stress, owing to its efficacy against Varroa mites and contributed to the recovery of bee colonies. In spite of its certain downsides, coumaphos remains an effective anti-varroa substance, but should be used with precaution, not to add to the effects of environmental factors which may cause red-ox misbalance., Procenjivana je efikasnost kumafosa protiv varoe, kao i njegov uticaj na oksidativni status i preživljavanje medonosne pčele (Apis mellifera). Ispitivanje je obavljeno na košnicama iz istog pčelinjaka, ujednačenim po broju pčela, površini legla i količini uskladištene hrane. Na osnovu infestacije varoom, košnice su podeljene u dve grupe: neinfestirane (N) i infestirane (I). Obe grupe su bile ili tretirane (T) – NT i IT, ili netretirane (U) – NU i IU. Tretman infestiranih društava je kontrolisan naknadnom primenom amitraza. Efikasnost kumafosa iznosila je 96-97%. Ovaj organofosfat imao Zikic et al.: Anti-varroa efficiency of coumaphos and its influence on oxidative stress and survival of honey bees 373 je zanemarljiv efekat na preživljavanje pčela, ali je značajno uticao na njihov oksidativni status: aktivnosti superoksid-dismutaze (SOD), katalaze (CAT) i glutation Stransferaze (GST), i koncentracije malonil-dialdehida (MDA). Kumafos je značajno (p˂0,0001) smanjio aktivnost SOD kod neinfestiranih pčela, ali ju je povećao kod infestiranih. Za razliku od toga, aktivnosti CAT i GST, kao i koncentracije MDA značajno su se povećale (od p˂0,05 do p˂0,0001) posle tretmana u svim grupama, sa izuzetkom IT, u kojoj su opale. Kumafos je sam po sebi u neinfestiranim društvima prouzrokovao oksidativni stres kod pčela, slično kao Varroa u infestiranim. Međutim, u infestiranim košnicama on je smanjio oksidativni stress zahvaljujući efikasnosti protiv varoe, čime je doprineo oporavku društava. Uprkos određenim nedostacima, kumafos ostaje efikasna supstanca u borbi protiv varoe, ali ga treba koristiti sa oprezom, da bi se izbeglo dodatno opterećenje pčela prouzrokovano faktorima sredine koji mogu da izazovu red-oks neravnotežu.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Anti-Varroa Efficiency of Coumaphos and Its Influence on Oxidative Stress and Survival of Honey Bees, Efikasnost kumafosa protiv varoe i njegov uticaj na oksidativni stres i preživljavanje medonosne pčele",
volume = "70",
number = "3",
pages = "355-373",
doi = "10.2478/acve-2020-0027"
}

Zikic, B., Aleksić, N., Ristanić, M., Glavinić, U., Vejnović, B., Krnjaić, I.,& Stanimirović, Z.. (2020). Anti-Varroa Efficiency of Coumaphos and Its Influence on Oxidative Stress and Survival of Honey Bees. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(3), 355-373.
https://doi.org/10.2478/acve-2020-0027

Zikic B, Aleksić N, Ristanić M, Glavinić U, Vejnović B, Krnjaić I, Stanimirović Z. Anti-Varroa Efficiency of Coumaphos and Its Influence on Oxidative Stress and Survival of Honey Bees. in Acta Veterinaria-Beograd. 2020;70(3):355-373.
doi:10.2478/acve-2020-0027 .

Zikic, Biljana, Aleksić, Nevenka, Ristanić, Marko, Glavinić, Uroš, Vejnović, Branislav, Krnjaić, Igor, Stanimirović, Zoran, "Anti-Varroa Efficiency of Coumaphos and Its Influence on Oxidative Stress and Survival of Honey Bees" in Acta Veterinaria-Beograd, 70, no. 3 (2020):355-373,
https://doi.org/10.2478/acve-2020-0027 . .

TY  - JOUR
AU  - Petrović, Slobodan
AU  - Maletić, Milan
AU  - Lakić, Nada
AU  - Aleksić, Nevenka
AU  - Maletić, Jelena
AU  - Ristanić, Marko
AU  - Stanimirović, Zoran
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2036
AB  - The aim of the current research was to assess the effects of the feed additive made
of lyophilised melon juice (source of superoxide dismutase, SOD) and inactivated live
Saccharomyces cerevisiae (strain R397) cells added to the feed via the product containing
high levels of organically bound selenium (source of selenium-dependant glutathione
peroxidase, Se-GPx) on the semen quality of bulls in heat stress conditions. The 15
bulls chosen for the experiment were assigned to three equal groups (control –group
C; treated group M, given the source of SOD; and group A, treated with the source
of Se-GPx). The research was conducted in summer. The activities of SOD and SeGPx in seminal plasma were determined spectrophotometrically. Computer-assisted
semen analysis was done to determine the sperm counts, motility and velocity. The
temperature and humidity were recorded with a digital data logger.
The average SOD activity in the control bulls was significantly lower than in M
(p<0.001) and A (p<0.001), whilst the average activities in the treated groups did not
differ significantly (p=0.784). Higher average SOD activity compared to the control in
the treated groups showed that both feed additives increased the antioxidative capacity
of the seminal fluid. The average GPx activity in the control was significantly lower
than in groups A (p=0.001) and M (p=0.005), whilst the two treatments did not lead to
significantly different results (p=0.701). The analysis of relations between the activity of
each enzyme and sperm motility and progressive motility in each of the bulls failed to
detect a significant correlation. The analysis of the relation between THI (temperaturehumidity index) and the activity of the antioxidative enzymes revealed that the increase
in THI coincided with the decrease in the SOD activity in the control group, but with
its increase in the treated groups (p>0.05). In all of the three groups with the increase
in THI there was an increase in GPx activity (p>0.05). It can be concluded that in all
of the three groups of bulls there was an increase in the activity of both enzymes in the seminal plasma, but the increase was significantly lower in the control. Thus, the
antioxidative capacity of the seminal plasma of untreated bulls was proven to be lower
in comparison with those of the treated animals.
AB  - Cilj ovog istraživanja bio je ispitivanje efekta liofi lizovanog preparata voćne pulpe dinje (izvor superoksid dismutase – SOD) i inaktivisanih živih ćelija kvasca Sacharomycess cere-visae (soj R397) dodatih u hranu kroz preparat koji sadrži visok nivo organski vezanog selena (izvor selen zavisne glutation-peroksidaze – Se-GPx) na kvalitet semena bikova u uslovima toplotnog stresa. U ogledje bilo uključeno 15 bikova podeljenih u tri grupe (kontrolna – C grupa, ogledna grupa-M kojoj je dodavan izvor SOD – i ogledna grupa –  A  tretirana  izvorom  Se-GPx).  Ogled  je  sproveden  u  letnjem  periodu.  Aktivnosti  SOD i Se-GPx u seminalnoj plazmi određivane su spektofotometrijski. Za utvrđivanje koncentracija spermatozoida, pokretljivosti i brzinskih parametara korišćena je CASA (computer-assisted semen analysis), a za merenje temeperature i vlažnosti koristili smo digi-talni data loger.Prosečna aktivnost SOD u kontrolnoj grupi bikova bila je značajno niža od one u M grupi (p<0,001) i A grupi (p<0,001), dok se prosečne vrednosti u tretiranim grupama nisu međusobno značajno razlikovale (p=0,784). Više prosečne vrednosti SOD su u tretiranim grupama u odnosu na kontrolnu ukazuju da su oba dodatka hrani povećala antioksidativni kapacitet semene plazme. Prosečna aktivnost GPx za kontrolnu grupu je bila značajno niža nego u A (p=0,001) i M grupi bikova (p=0.005), dok se među-sobno M i A grupa nisu značajno razlikovale (p=0,701). Analizom korelacije između aktivnosti svakog od enzima i procenta pokretljivih i progresivno pokretljivih sperma-tozoida kod svakog bika ponaosob nije ustanovljena značajna povezanost. Analizom veze  između  THI  (temperature-humidity  index)  i  aktivnosti  antioksidativnih  enzima  uočeno  je  da  je  sa  porastom  THI  u  kontrolnoj  grupi  došlo  do  smanjenja  aktivnosti  SOD, dok je u tretiranim grupama nastalo povećanje aktivnosti SOD (p>0.05). U sve tri  grupe  bikova  povećanjem  THI  povećavala  se  i  aktivnost  GPx  (p>0.05).  Može  se  zaključiti da je kod sve tri grupe bikova došlo do povećanja aktivnosti oba enzima u semenoj plazmi, ali je povećanje značajno niže u kontrolnoj grupi nego u tretiranim grupama. Time je dokazan manji antioksidativni kapacitet semene plazme netretiranih bikova u odnosu na tretirane.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions
T1  - Efekat dodavanja antioksidanasa u hrani na određene parametre kvaliteta semena bikova u uslovima toplotnog stresa
VL  - 70
IS  - 4
SP  - 453
EP  - 470
DO  - 10.2478/acve-2020-0034
ER  - 

@article{
author = "Petrović, Slobodan and Maletić, Milan and Lakić, Nada and Aleksić, Nevenka and Maletić, Jelena and Ristanić, Marko and Stanimirović, Zoran",
year = "2020",
abstract = "The aim of the current research was to assess the effects of the feed additive made
of lyophilised melon juice (source of superoxide dismutase, SOD) and inactivated live
Saccharomyces cerevisiae (strain R397) cells added to the feed via the product containing
high levels of organically bound selenium (source of selenium-dependant glutathione
peroxidase, Se-GPx) on the semen quality of bulls in heat stress conditions. The 15
bulls chosen for the experiment were assigned to three equal groups (control –group
C; treated group M, given the source of SOD; and group A, treated with the source
of Se-GPx). The research was conducted in summer. The activities of SOD and SeGPx in seminal plasma were determined spectrophotometrically. Computer-assisted
semen analysis was done to determine the sperm counts, motility and velocity. The
temperature and humidity were recorded with a digital data logger.
The average SOD activity in the control bulls was significantly lower than in M
(p<0.001) and A (p<0.001), whilst the average activities in the treated groups did not
differ significantly (p=0.784). Higher average SOD activity compared to the control in
the treated groups showed that both feed additives increased the antioxidative capacity
of the seminal fluid. The average GPx activity in the control was significantly lower
than in groups A (p=0.001) and M (p=0.005), whilst the two treatments did not lead to
significantly different results (p=0.701). The analysis of relations between the activity of
each enzyme and sperm motility and progressive motility in each of the bulls failed to
detect a significant correlation. The analysis of the relation between THI (temperaturehumidity index) and the activity of the antioxidative enzymes revealed that the increase
in THI coincided with the decrease in the SOD activity in the control group, but with
its increase in the treated groups (p>0.05). In all of the three groups with the increase
in THI there was an increase in GPx activity (p>0.05). It can be concluded that in all
of the three groups of bulls there was an increase in the activity of both enzymes in the seminal plasma, but the increase was significantly lower in the control. Thus, the
antioxidative capacity of the seminal plasma of untreated bulls was proven to be lower
in comparison with those of the treated animals., Cilj ovog istraživanja bio je ispitivanje efekta liofi lizovanog preparata voćne pulpe dinje (izvor superoksid dismutase – SOD) i inaktivisanih živih ćelija kvasca Sacharomycess cere-visae (soj R397) dodatih u hranu kroz preparat koji sadrži visok nivo organski vezanog selena (izvor selen zavisne glutation-peroksidaze – Se-GPx) na kvalitet semena bikova u uslovima toplotnog stresa. U ogledje bilo uključeno 15 bikova podeljenih u tri grupe (kontrolna – C grupa, ogledna grupa-M kojoj je dodavan izvor SOD – i ogledna grupa –  A  tretirana  izvorom  Se-GPx).  Ogled  je  sproveden  u  letnjem  periodu.  Aktivnosti  SOD i Se-GPx u seminalnoj plazmi određivane su spektofotometrijski. Za utvrđivanje koncentracija spermatozoida, pokretljivosti i brzinskih parametara korišćena je CASA (computer-assisted semen analysis), a za merenje temeperature i vlažnosti koristili smo digi-talni data loger.Prosečna aktivnost SOD u kontrolnoj grupi bikova bila je značajno niža od one u M grupi (p<0,001) i A grupi (p<0,001), dok se prosečne vrednosti u tretiranim grupama nisu međusobno značajno razlikovale (p=0,784). Više prosečne vrednosti SOD su u tretiranim grupama u odnosu na kontrolnu ukazuju da su oba dodatka hrani povećala antioksidativni kapacitet semene plazme. Prosečna aktivnost GPx za kontrolnu grupu je bila značajno niža nego u A (p=0,001) i M grupi bikova (p=0.005), dok se među-sobno M i A grupa nisu značajno razlikovale (p=0,701). Analizom korelacije između aktivnosti svakog od enzima i procenta pokretljivih i progresivno pokretljivih sperma-tozoida kod svakog bika ponaosob nije ustanovljena značajna povezanost. Analizom veze  između  THI  (temperature-humidity  index)  i  aktivnosti  antioksidativnih  enzima  uočeno  je  da  je  sa  porastom  THI  u  kontrolnoj  grupi  došlo  do  smanjenja  aktivnosti  SOD, dok je u tretiranim grupama nastalo povećanje aktivnosti SOD (p>0.05). U sve tri  grupe  bikova  povećanjem  THI  povećavala  se  i  aktivnost  GPx  (p>0.05).  Može  se  zaključiti da je kod sve tri grupe bikova došlo do povećanja aktivnosti oba enzima u semenoj plazmi, ali je povećanje značajno niže u kontrolnoj grupi nego u tretiranim grupama. Time je dokazan manji antioksidativni kapacitet semene plazme netretiranih bikova u odnosu na tretirane.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions, Efekat dodavanja antioksidanasa u hrani na određene parametre kvaliteta semena bikova u uslovima toplotnog stresa",
volume = "70",
number = "4",
pages = "453-470",
doi = "10.2478/acve-2020-0034"
}

Petrović, S., Maletić, M., Lakić, N., Aleksić, N., Maletić, J., Ristanić, M.,& Stanimirović, Z.. (2020). The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(4), 453-470.
https://doi.org/10.2478/acve-2020-0034

Petrović S, Maletić M, Lakić N, Aleksić N, Maletić J, Ristanić M, Stanimirović Z. The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions. in Acta Veterinaria-Beograd. 2020;70(4):453-470.
doi:10.2478/acve-2020-0034 .

Petrović, Slobodan, Maletić, Milan, Lakić, Nada, Aleksić, Nevenka, Maletić, Jelena, Ristanić, Marko, Stanimirović, Zoran, "The effects of antioxidants provided with feed on certain quality parameters of bull semen under heat stress conditions" in Acta Veterinaria-Beograd, 70, no. 4 (2020):453-470,
https://doi.org/10.2478/acve-2020-0034 . .

TY  - JOUR
AU  - Vučićević, Miloš
AU  - Vučićević, Ivana
AU  - Došenović, Milan
AU  - Ristanić, Marko
AU  - Aleksić, Nevenka
AU  - Resanović, Radmila
AU  - Stanimirović, Zoran
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2038
AB  - Eight lovebirds of both sexes and different age were admitted showing alterations in
behaviour and apathy. During the initial examination delamination of the beak was
noted in all birds and discrete areas of alopecia in three of eight birds. Based on clinical
experience, molecular diagnostic on PBFD, sequencing of obtained amplicons, and
histopathological examination were performed. All birds tested positive on the presence
of PBFD virus, despite the fact they did not exhibit expected clinical signs for PBFD.
Sequencing results showed 100% match with sequences previously isolated from parrots
with PBFD exhibiting classical PBFD manifestation. Histopathological examination
showed similar findings as in previously described cases of PBFD in parrots. Other
studies on psittacine birds correlate clinical manifestations and nucleotide variations
with geographic localization. Our results indicate that the clinical manifestation of the
disease is more dependent on bird species than on the genetic variation of the virus or
the geographical distribution.
AB  - Osam rozenkolisa oba pola različite starosti su primljeni na pregled zbog uočenih promena u ponašanju i apatije. Tokom inicijalnog pregleda kod svih ptica je uočena delaminacija kljuna, a kod tri jedinke bila su prisutna i diskretna polja alopecije. Na osnovu
kliničkog iskustva postavljena je sumnja na oboljenje bolest kljuna i perja te je urađena
molekularna dijagnostika na pristutvo virusa bolesti kljuna i perja, sekvencioniranje
dobijenih amplifikata i patohistološko ispitivanje. Rezultati ispitivanja su potvrdili prisustvo virusa u uzorcima od svih ispitivanih jedinki, poklapanje nukleotidnih sekvecni
od 100% sa uzorcima od drugih ptica sa drugačijom kliničkom slikom, a patohistološke promene su identične onima opisanim kod bolesti kljuna i perja. Studije brojnih
autora povezivale su kliničku manifestaciju bolesti sa nukleotidnim varijacijama ili geografskom lokalizacijom. Međutim, opisani rezultati bi mogli da ukazuju da je kliničko
ispoljavanje bolesti više zavisno od vrste domaćina nego od genotipa virusa koji bolest
izaziva i od teritorije na kojoj se bolest ispoljava.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Is PBFD simptomatology species specific rather then strain specific? – a case of 8 lovebirds
T1  - Da li je simptomatologija PBFD više zavisna od vrste domaćina nego od soja virusa? – Slučaj 8 rozenkolisa
VL  - 70
IS  - 3
SP  - 386
EP  - 394
DO  - 10.2478/acve-2020-0029
ER  - 

@article{
author = "Vučićević, Miloš and Vučićević, Ivana and Došenović, Milan and Ristanić, Marko and Aleksić, Nevenka and Resanović, Radmila and Stanimirović, Zoran",
year = "2020",
abstract = "Eight lovebirds of both sexes and different age were admitted showing alterations in
behaviour and apathy. During the initial examination delamination of the beak was
noted in all birds and discrete areas of alopecia in three of eight birds. Based on clinical
experience, molecular diagnostic on PBFD, sequencing of obtained amplicons, and
histopathological examination were performed. All birds tested positive on the presence
of PBFD virus, despite the fact they did not exhibit expected clinical signs for PBFD.
Sequencing results showed 100% match with sequences previously isolated from parrots
with PBFD exhibiting classical PBFD manifestation. Histopathological examination
showed similar findings as in previously described cases of PBFD in parrots. Other
studies on psittacine birds correlate clinical manifestations and nucleotide variations
with geographic localization. Our results indicate that the clinical manifestation of the
disease is more dependent on bird species than on the genetic variation of the virus or
the geographical distribution., Osam rozenkolisa oba pola različite starosti su primljeni na pregled zbog uočenih promena u ponašanju i apatije. Tokom inicijalnog pregleda kod svih ptica je uočena delaminacija kljuna, a kod tri jedinke bila su prisutna i diskretna polja alopecije. Na osnovu
kliničkog iskustva postavljena je sumnja na oboljenje bolest kljuna i perja te je urađena
molekularna dijagnostika na pristutvo virusa bolesti kljuna i perja, sekvencioniranje
dobijenih amplifikata i patohistološko ispitivanje. Rezultati ispitivanja su potvrdili prisustvo virusa u uzorcima od svih ispitivanih jedinki, poklapanje nukleotidnih sekvecni
od 100% sa uzorcima od drugih ptica sa drugačijom kliničkom slikom, a patohistološke promene su identične onima opisanim kod bolesti kljuna i perja. Studije brojnih
autora povezivale su kliničku manifestaciju bolesti sa nukleotidnim varijacijama ili geografskom lokalizacijom. Međutim, opisani rezultati bi mogli da ukazuju da je kliničko
ispoljavanje bolesti više zavisno od vrste domaćina nego od genotipa virusa koji bolest
izaziva i od teritorije na kojoj se bolest ispoljava.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Is PBFD simptomatology species specific rather then strain specific? – a case of 8 lovebirds, Da li je simptomatologija PBFD više zavisna od vrste domaćina nego od soja virusa? – Slučaj 8 rozenkolisa",
volume = "70",
number = "3",
pages = "386-394",
doi = "10.2478/acve-2020-0029"
}

Vučićević, M., Vučićević, I., Došenović, M., Ristanić, M., Aleksić, N., Resanović, R.,& Stanimirović, Z.. (2020). Is PBFD simptomatology species specific rather then strain specific? – a case of 8 lovebirds. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(3), 386-394.
https://doi.org/10.2478/acve-2020-0029

Vučićević M, Vučićević I, Došenović M, Ristanić M, Aleksić N, Resanović R, Stanimirović Z. Is PBFD simptomatology species specific rather then strain specific? – a case of 8 lovebirds. in Acta Veterinaria-Beograd. 2020;70(3):386-394.
doi:10.2478/acve-2020-0029 .

Vučićević, Miloš, Vučićević, Ivana, Došenović, Milan, Ristanić, Marko, Aleksić, Nevenka, Resanović, Radmila, Stanimirović, Zoran, "Is PBFD simptomatology species specific rather then strain specific? – a case of 8 lovebirds" in Acta Veterinaria-Beograd, 70, no. 3 (2020):386-394,
https://doi.org/10.2478/acve-2020-0029 . .

TY  - JOUR
AU  - Podpecan, Ozbalt
AU  - Zrimsek, Petra
AU  - Mrkun, Janko
AU  - Golicnik, Marko
AU  - Radovanović, Anita
AU  - Jovanović, Ljubomir
AU  - Vujanac, Ivan
AU  - Prodanović, Radiša
AU  - Kirovski, Danijela
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1823
AB  - The objective was to determine optimal thresholds of blood variables that are associated with liver lipid and glycogen content in dairy cows. Liver specimens of 15 Holstein dairy cows were taken 2 weeks postpartum (PP) and used for determination of lipid and glycogen content, respectively. Blood samples were collected weekly, starting from week 2 antepartum (AP) up to week 2 PP. Selected blood parameters and body condition scoring (BCS) were evaluated through receiver operating characteristic curve analysis as indication of fat and glycogen content in the liver. Postpartal low liver glycogen content (mode < 2.0, semiquantitative analysis of glycogen content in the liver tissue samples) can be indicated if values are higher than 0.16 mmol/L, and 3.5 mmol/L for NEFA, and glucose, respectively, and lower than 0.65 mmol/L and 17.94 mu U/mL for BHBA and insulin, respectively, 2 weeks AP. Postaprtal high liver fat content (>20%, stereological quantification of fat content in the liver tissue samples) can be indicated if values are higher than 3.5 for BCS at week 2 AP. Optimal time for indicating both low liver glycogen/high fat content is 1 week PP, with LDL < cut off point set at 0.18 mmol/L and BHBA > cut off point set at 0.88 mmol/L. Established cut off values in this study can be used for indication of fat and glycogen content in the liver of dairy cows.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Italian Journal of Animal Science
T1  - Tresholds of blood variables obtained by receiver operating characteristic analysis for indication of fat and glycogen content in the liver of postpartum dairy cows
VL  - 19
IS  - 1
SP  - 303
EP  - 309
DO  - 10.1080/1828051X.2020.1740064
ER  - 

@article{
author = "Podpecan, Ozbalt and Zrimsek, Petra and Mrkun, Janko and Golicnik, Marko and Radovanović, Anita and Jovanović, Ljubomir and Vujanac, Ivan and Prodanović, Radiša and Kirovski, Danijela",
year = "2020",
abstract = "The objective was to determine optimal thresholds of blood variables that are associated with liver lipid and glycogen content in dairy cows. Liver specimens of 15 Holstein dairy cows were taken 2 weeks postpartum (PP) and used for determination of lipid and glycogen content, respectively. Blood samples were collected weekly, starting from week 2 antepartum (AP) up to week 2 PP. Selected blood parameters and body condition scoring (BCS) were evaluated through receiver operating characteristic curve analysis as indication of fat and glycogen content in the liver. Postpartal low liver glycogen content (mode < 2.0, semiquantitative analysis of glycogen content in the liver tissue samples) can be indicated if values are higher than 0.16 mmol/L, and 3.5 mmol/L for NEFA, and glucose, respectively, and lower than 0.65 mmol/L and 17.94 mu U/mL for BHBA and insulin, respectively, 2 weeks AP. Postaprtal high liver fat content (>20%, stereological quantification of fat content in the liver tissue samples) can be indicated if values are higher than 3.5 for BCS at week 2 AP. Optimal time for indicating both low liver glycogen/high fat content is 1 week PP, with LDL < cut off point set at 0.18 mmol/L and BHBA > cut off point set at 0.88 mmol/L. Established cut off values in this study can be used for indication of fat and glycogen content in the liver of dairy cows.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Italian Journal of Animal Science",
title = "Tresholds of blood variables obtained by receiver operating characteristic analysis for indication of fat and glycogen content in the liver of postpartum dairy cows",
volume = "19",
number = "1",
pages = "303-309",
doi = "10.1080/1828051X.2020.1740064"
}

Podpecan, O., Zrimsek, P., Mrkun, J., Golicnik, M., Radovanović, A., Jovanović, L., Vujanac, I., Prodanović, R.,& Kirovski, D.. (2020). Tresholds of blood variables obtained by receiver operating characteristic analysis for indication of fat and glycogen content in the liver of postpartum dairy cows. in Italian Journal of Animal Science
Taylor & Francis Ltd, Abingdon., 19(1), 303-309.
https://doi.org/10.1080/1828051X.2020.1740064

Podpecan O, Zrimsek P, Mrkun J, Golicnik M, Radovanović A, Jovanović L, Vujanac I, Prodanović R, Kirovski D. Tresholds of blood variables obtained by receiver operating characteristic analysis for indication of fat and glycogen content in the liver of postpartum dairy cows. in Italian Journal of Animal Science. 2020;19(1):303-309.
doi:10.1080/1828051X.2020.1740064 .

Podpecan, Ozbalt, Zrimsek, Petra, Mrkun, Janko, Golicnik, Marko, Radovanović, Anita, Jovanović, Ljubomir, Vujanac, Ivan, Prodanović, Radiša, Kirovski, Danijela, "Tresholds of blood variables obtained by receiver operating characteristic analysis for indication of fat and glycogen content in the liver of postpartum dairy cows" in Italian Journal of Animal Science, 19, no. 1 (2020):303-309,
https://doi.org/10.1080/1828051X.2020.1740064 . .

TY  - JOUR
AU  - Drašković, Vladimir
AU  - Stanimirović, Zoran
AU  - Glišić, Milica
AU  - Bošnjak-Neumuller, Jasna
AU  - Teodorović, Radislava
AU  - Teodorović, Vlado
AU  - Kukolj, Vladimir
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1810
AB  - Proliferative enteropathy, also known as proliferative ileitis, is considered to be one of the most economically important diseases in pig production worldwide. The estimated losses per affected growing pig usually range from US $1 to $5. The disease is caused by Lawsonia intrawellularis, a Gram-negative, obligately intracellular bacterium. Control of the disease can be achieved with the use of vaccines or antibiotics. Recently there has been an increase in the efforts in the control of certain pathologies of the digestive system with phytogenic additives. The aim of this work was to assess the effects of a phytogenic additive on the histomorphometric characteristics of the intestines in weaned pigs with a subclinical infection with L. intracellularis acquired spontaneously. Histomorphometry analysis showed that crypt depth was significantly shorter (P <0.05), and the villus-height-to-crypt-depth ratio (P<0.05) significantly greater in the treatment group than the control. This improvement in the histological parameters of the intestine, considered to be indicators of its health, proved the positive effect of the tested additive on the digestive system in pigs.
AB  - Proliferativna enteropatija poznata kao i proliferativni ileitis smatra se jednom od ekonomski najznačajnih bolesti u svinjarskoj proizvodnji širom sveta. Procenjeni gubici
po obolelom tovljeniku obično se kreću od 1 do 5 američkih dolara. Uzročnik ovog
oboljenja je Lawsonia intracellularis, gram negativna obligatna intracelularna bakterija.
Kontrola ovog oboljenja ostvaruje se primenom vakcinacije ili antibiotika. U poslednje
vreme sve je češća primena fi togenih aditiva u cilju kontolisanja određenih patoloških
stanja digestivnog trakta. Cilj ovog rada bio je da se ustanove efekti fi togenog aditiva na
histomorfometrijske karakteristike creva kod odlučenih prasadi supklinički prirodno
infi ciranih L. intracellularis. Histomorfometrijska analiza pokazala je da su kripte bile
značajno (P<0,05) pliće, a količnik visine vilusa i dubine kripti značajno veći (P<0,05)
u tretiranoj grupi prasadi nego u kontroli. Ovo poboljšanje histoloških parametara
creva, koje se smatraju indikatorima njegovog zdravlja, dokaz je pozitivnog efekta ispitivanog aditiva na digestivni sistem svinja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The effects of a phytogenic additive on the histomorphometric characteristics of the intestines in weaned pigs with a subclinical natural infection with lawsonia intracellularis
T1  - Efekti fitogenog aditiva na histomorfometrijske karakteristike creva kod odlučene prasadi supklinički prirodno inficirane bakterijom lawsonia intracellularis
VL  - 70
IS  - 1
SP  - 81
EP  - 91
DO  - 10.2478/acve-2020-0006
ER  - 

@article{
author = "Drašković, Vladimir and Stanimirović, Zoran and Glišić, Milica and Bošnjak-Neumuller, Jasna and Teodorović, Radislava and Teodorović, Vlado and Kukolj, Vladimir",
year = "2020",
abstract = "Proliferative enteropathy, also known as proliferative ileitis, is considered to be one of the most economically important diseases in pig production worldwide. The estimated losses per affected growing pig usually range from US $1 to $5. The disease is caused by Lawsonia intrawellularis, a Gram-negative, obligately intracellular bacterium. Control of the disease can be achieved with the use of vaccines or antibiotics. Recently there has been an increase in the efforts in the control of certain pathologies of the digestive system with phytogenic additives. The aim of this work was to assess the effects of a phytogenic additive on the histomorphometric characteristics of the intestines in weaned pigs with a subclinical infection with L. intracellularis acquired spontaneously. Histomorphometry analysis showed that crypt depth was significantly shorter (P <0.05), and the villus-height-to-crypt-depth ratio (P<0.05) significantly greater in the treatment group than the control. This improvement in the histological parameters of the intestine, considered to be indicators of its health, proved the positive effect of the tested additive on the digestive system in pigs., Proliferativna enteropatija poznata kao i proliferativni ileitis smatra se jednom od ekonomski najznačajnih bolesti u svinjarskoj proizvodnji širom sveta. Procenjeni gubici
po obolelom tovljeniku obično se kreću od 1 do 5 američkih dolara. Uzročnik ovog
oboljenja je Lawsonia intracellularis, gram negativna obligatna intracelularna bakterija.
Kontrola ovog oboljenja ostvaruje se primenom vakcinacije ili antibiotika. U poslednje
vreme sve je češća primena fi togenih aditiva u cilju kontolisanja određenih patoloških
stanja digestivnog trakta. Cilj ovog rada bio je da se ustanove efekti fi togenog aditiva na
histomorfometrijske karakteristike creva kod odlučenih prasadi supklinički prirodno
infi ciranih L. intracellularis. Histomorfometrijska analiza pokazala je da su kripte bile
značajno (P<0,05) pliće, a količnik visine vilusa i dubine kripti značajno veći (P<0,05)
u tretiranoj grupi prasadi nego u kontroli. Ovo poboljšanje histoloških parametara
creva, koje se smatraju indikatorima njegovog zdravlja, dokaz je pozitivnog efekta ispitivanog aditiva na digestivni sistem svinja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The effects of a phytogenic additive on the histomorphometric characteristics of the intestines in weaned pigs with a subclinical natural infection with lawsonia intracellularis, Efekti fitogenog aditiva na histomorfometrijske karakteristike creva kod odlučene prasadi supklinički prirodno inficirane bakterijom lawsonia intracellularis",
volume = "70",
number = "1",
pages = "81-91",
doi = "10.2478/acve-2020-0006"
}

Drašković, V., Stanimirović, Z., Glišić, M., Bošnjak-Neumuller, J., Teodorović, R., Teodorović, V.,& Kukolj, V.. (2020). The effects of a phytogenic additive on the histomorphometric characteristics of the intestines in weaned pigs with a subclinical natural infection with lawsonia intracellularis. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(1), 81-91.
https://doi.org/10.2478/acve-2020-0006

Drašković V, Stanimirović Z, Glišić M, Bošnjak-Neumuller J, Teodorović R, Teodorović V, Kukolj V. The effects of a phytogenic additive on the histomorphometric characteristics of the intestines in weaned pigs with a subclinical natural infection with lawsonia intracellularis. in Acta Veterinaria-Beograd. 2020;70(1):81-91.
doi:10.2478/acve-2020-0006 .

Drašković, Vladimir, Stanimirović, Zoran, Glišić, Milica, Bošnjak-Neumuller, Jasna, Teodorović, Radislava, Teodorović, Vlado, Kukolj, Vladimir, "The effects of a phytogenic additive on the histomorphometric characteristics of the intestines in weaned pigs with a subclinical natural infection with lawsonia intracellularis" in Acta Veterinaria-Beograd, 70, no. 1 (2020):81-91,
https://doi.org/10.2478/acve-2020-0006 . .

TY  - CONF
AU  - Radulović, Stamen
AU  - Šefer, Dragan
AU  - Marković, Radmila
AU  - Perić, Dejan
AU  - Bošković, Saša
AU  - Milićević, Dragan
AU  - Lukić, Mirjana
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3460
AB  - Functional food can be considered food that has been scientifically proven to have
a positive effect on certain body functions (in addition to the usual nutritional
value) that contribute to human health and reduce the risk of disease. Food
functionality is achieved by the presence of bioactive components (one or more)
that have been scientifically proven to have positive effects on human health,
participate in maintaining optimal gastrointestinal functions, raising levels of
antioxidant defenses, as well as reducing risk factors involved in the etiology of
cardiovascular disease and cancer. The attention of the professional and scientific
public today is focused on the production of designed products of animal origin,
primarily meat and milk, which due to the specific diet of animals are usually
enriched with minerals (selenium), vitamins (vitamin E) and fats (omega-3 fatty
acids). The procedure for the production of named products implies a good
knowledge of the technique of formulating feed meals for animal nutrition, with
special emphasis on the possibility of utilization of those (added) substances in
their body. Thus, when using mineral substances, the form in which they are
found is of crucial importance, as well as the applied quantities which must not
endanger the health status of the animals. Among the traditionally used inorganic
forms of minerals in animal nutrition, sulfates show a number of advantages over chlorides and/or carbonates. Modern nutritional techniques provide the
possibility of using new chelated forms, hydroxy forms, as well as nano forms of
mineral substances, which achieves significant improvements in terms of their
absorption in the digestive tract of treated animals. When using fat, it is necessary
to know the degree of functional development of the individual's digestive tract, as
well as basic characteristics such as the length of the carbon chain and the degree
of fatty acid saturation, which have been shown to have a great influence on
optimal digestion and absorption of fat. Also, fats originating from feed, directly
and indirectly modulate the intestinal microbiota of the host, by creating
appropriate conditions favorable or unfavorable for the growth and reproduction
of certain bacterial species.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 25. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), naučni skup sa međunarodnim učešćem, Teslić,  2020
T1  - Nutritivne tehnike, i specifičnosti u proizvodnji funkcionalne hrane
T1  - Nutritive techniques and specificities in functional food production
SP  - 176
EP  - 179
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3460
ER  - 

@conference{
author = "Radulović, Stamen and Šefer, Dragan and Marković, Radmila and Perić, Dejan and Bošković, Saša and Milićević, Dragan and Lukić, Mirjana",
year = "2020",
abstract = "Functional food can be considered food that has been scientifically proven to have
a positive effect on certain body functions (in addition to the usual nutritional
value) that contribute to human health and reduce the risk of disease. Food
functionality is achieved by the presence of bioactive components (one or more)
that have been scientifically proven to have positive effects on human health,
participate in maintaining optimal gastrointestinal functions, raising levels of
antioxidant defenses, as well as reducing risk factors involved in the etiology of
cardiovascular disease and cancer. The attention of the professional and scientific
public today is focused on the production of designed products of animal origin,
primarily meat and milk, which due to the specific diet of animals are usually
enriched with minerals (selenium), vitamins (vitamin E) and fats (omega-3 fatty
acids). The procedure for the production of named products implies a good
knowledge of the technique of formulating feed meals for animal nutrition, with
special emphasis on the possibility of utilization of those (added) substances in
their body. Thus, when using mineral substances, the form in which they are
found is of crucial importance, as well as the applied quantities which must not
endanger the health status of the animals. Among the traditionally used inorganic
forms of minerals in animal nutrition, sulfates show a number of advantages over chlorides and/or carbonates. Modern nutritional techniques provide the
possibility of using new chelated forms, hydroxy forms, as well as nano forms of
mineral substances, which achieves significant improvements in terms of their
absorption in the digestive tract of treated animals. When using fat, it is necessary
to know the degree of functional development of the individual's digestive tract, as
well as basic characteristics such as the length of the carbon chain and the degree
of fatty acid saturation, which have been shown to have a great influence on
optimal digestion and absorption of fat. Also, fats originating from feed, directly
and indirectly modulate the intestinal microbiota of the host, by creating
appropriate conditions favorable or unfavorable for the growth and reproduction
of certain bacterial species.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "25. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), naučni skup sa međunarodnim učešćem, Teslić,  2020",
title = "Nutritivne tehnike, i specifičnosti u proizvodnji funkcionalne hrane, Nutritive techniques and specificities in functional food production",
pages = "176-179",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3460"
}

Radulović, S., Šefer, D., Marković, R., Perić, D., Bošković, S., Milićević, D.,& Lukić, M.. (2020). Nutritivne tehnike, i specifičnosti u proizvodnji funkcionalne hrane. in 25. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), naučni skup sa međunarodnim učešćem, Teslić,  2020
Banja Luka : Veterinarska komora Republike Srpske., 176-179.
https://hdl.handle.net/21.15107/rcub_veterinar_3460

Radulović S, Šefer D, Marković R, Perić D, Bošković S, Milićević D, Lukić M. Nutritivne tehnike, i specifičnosti u proizvodnji funkcionalne hrane. in 25. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), naučni skup sa međunarodnim učešćem, Teslić,  2020. 2020;:176-179.
https://hdl.handle.net/21.15107/rcub_veterinar_3460 .

Radulović, Stamen, Šefer, Dragan, Marković, Radmila, Perić, Dejan, Bošković, Saša, Milićević, Dragan, Lukić, Mirjana, "Nutritivne tehnike, i specifičnosti u proizvodnji funkcionalne hrane" in 25. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), naučni skup sa međunarodnim učešćem, Teslić,  2020 (2020):176-179,
https://hdl.handle.net/21.15107/rcub_veterinar_3460 .

TY  - JOUR
AU  - Stanišić, Ljubodrag
AU  - Aleksić, Jelena M.
AU  - Dimitrijević, Vladimir
AU  - Kovačević, Branislav
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1808
AB  - The dominant donkey breed in the Balkans is the mid-sized Balkan donkey with a grey to chocolate coat color. Local breeders from Serbia, however, still maintain a few larger individuals of a lighter coat color, named Banat donkey, and speculate that they are descendants of a Spanish donkey heard that had been transferred to the Banat region by the Hapsburg Queen Maria Theresa in the XVIII century for a specific purpose, to work in local vineyards. We have previously found a unique nuclear gene-pool and a prevalence of mitochondrial Clade 2 haplotypes in several such animals. In this study, we: (i) perform a comparative analysis of 18 morphological traits of the Banat donkey (seven individuals), Balkan donkey (53 individuals from two sub-populations of this breed) and the potential hybrids (eight individuals), and demonstrate the morphological distinctiveness of the Banat donkey, highlighting the diagnostic traits for distinguishing the breed: hip height, croup width, body length and chest depth; (ii) reanalyse published nuclear microsatellite data for these groups, and reveal that, although severely depopulated, the genetically distinct Banat donkey is not severely affected by the loss of genetic diversity and inbreeding; (iii) demonstrate that previously published Banat donkey mitochondrial haplotypes, analyzed genealogically together with those reported in ancient and modern individuals from Spain, Italy, Turkey, Cyprus and Africa, are shared with three Spanish breeds and individuals belonging to Amiata and some other Italian breeds. A unique morphological feature present in Banat and Somali wild donkeys, but also in Amiata donkeys, black stripes on legs, suggests that the origin of Clade 2 donkeys may be much more complex than previously thought. Actions to preserve the Banat donkey, a valuable but critically endangered genetic resource (<100 individuals), are urgent.
PB  - Peerj Inc, London
T2  - PEERJ
T1  - Banat donkey, a neglected donkey breed from the central Balkans (Serbia)
VL  - 8
SP  - 8598
DO  - 10.7717/peerj.8598
ER  - 

@article{
author = "Stanišić, Ljubodrag and Aleksić, Jelena M. and Dimitrijević, Vladimir and Kovačević, Branislav and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2020",
abstract = "The dominant donkey breed in the Balkans is the mid-sized Balkan donkey with a grey to chocolate coat color. Local breeders from Serbia, however, still maintain a few larger individuals of a lighter coat color, named Banat donkey, and speculate that they are descendants of a Spanish donkey heard that had been transferred to the Banat region by the Hapsburg Queen Maria Theresa in the XVIII century for a specific purpose, to work in local vineyards. We have previously found a unique nuclear gene-pool and a prevalence of mitochondrial Clade 2 haplotypes in several such animals. In this study, we: (i) perform a comparative analysis of 18 morphological traits of the Banat donkey (seven individuals), Balkan donkey (53 individuals from two sub-populations of this breed) and the potential hybrids (eight individuals), and demonstrate the morphological distinctiveness of the Banat donkey, highlighting the diagnostic traits for distinguishing the breed: hip height, croup width, body length and chest depth; (ii) reanalyse published nuclear microsatellite data for these groups, and reveal that, although severely depopulated, the genetically distinct Banat donkey is not severely affected by the loss of genetic diversity and inbreeding; (iii) demonstrate that previously published Banat donkey mitochondrial haplotypes, analyzed genealogically together with those reported in ancient and modern individuals from Spain, Italy, Turkey, Cyprus and Africa, are shared with three Spanish breeds and individuals belonging to Amiata and some other Italian breeds. A unique morphological feature present in Banat and Somali wild donkeys, but also in Amiata donkeys, black stripes on legs, suggests that the origin of Clade 2 donkeys may be much more complex than previously thought. Actions to preserve the Banat donkey, a valuable but critically endangered genetic resource (<100 individuals), are urgent.",
publisher = "Peerj Inc, London",
journal = "PEERJ",
title = "Banat donkey, a neglected donkey breed from the central Balkans (Serbia)",
volume = "8",
pages = "8598",
doi = "10.7717/peerj.8598"
}

Stanišić, L., Aleksić, J. M., Dimitrijević, V., Kovačević, B., Stevanović, J.,& Stanimirović, Z.. (2020). Banat donkey, a neglected donkey breed from the central Balkans (Serbia). in PEERJ
Peerj Inc, London., 8, 8598.
https://doi.org/10.7717/peerj.8598

Stanišić L, Aleksić JM, Dimitrijević V, Kovačević B, Stevanović J, Stanimirović Z. Banat donkey, a neglected donkey breed from the central Balkans (Serbia). in PEERJ. 2020;8:8598.
doi:10.7717/peerj.8598 .

Stanišić, Ljubodrag, Aleksić, Jelena M., Dimitrijević, Vladimir, Kovačević, Branislav, Stevanović, Jevrosima, Stanimirović, Zoran, "Banat donkey, a neglected donkey breed from the central Balkans (Serbia)" in PEERJ, 8 (2020):8598,
https://doi.org/10.7717/peerj.8598 . .

TY  - JOUR
AU  - Dimitrijević, Vladimir
AU  - Savić, Mila
AU  - Tarić, Elmin
AU  - Stanišić, Ljubodrag
AU  - Stanimirović, Zoran
AU  - Tabaković, Aleksandar
AU  - Aleksić, M. Jelena
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1866
AB  - Yugoslavian Shepherd Dog-Sharplanina (YSD) is a livestock guard dog from the Western Balkans present in this region over a long time, but recognized by the Fédération Cynologique Internationale as a distinct breed as late as 1957. However, the information regarding the origin and the size of the breed's foundation stock is still lacking. In order to contribute towards better understanding of the genetic make-up of the YSD and its foundation stock, we re-analyzed previously generated genetic profiles of 94 registered YSD dogs assessed with nine nuclear microsatellites. Studied individuals comprised 90 unrelated dogs and two pairs of full-sibs, sampled at four sampling sites: Three dog shows and at a military training centre for dogs in Serbia. We supported earlier findings on high levels of genetic diversity in YSD (HE=0.728±0.027) and lack of inbreeding, and revealed substructure of the breed because we found two distinct gene pools in the Bayesian clustering analysis, indicated also by the excess of homozygotes (i.e., Wahlund effect) and outcomes of other analyses: Linkage disequilibrium tests, Neighbour-Joining tree, principal coordinates and two-dimensional scaling analyses. The two gene pools were almost equally represented at each sampling site. One gene pool was composed of individuals with high genetic integrity, while the other gene pool was characterized with admixed ancestry, developed possibly via hybridization with native breeding stock outside the registry system, other breeds, such as the Caucasian Shepherd, and/or individuals admixed with wolves. Thus, we demonstrate rather complex and diverse ancestry implying a genetically heterogeneous foundation stock of the YSD.
AB  - Jugoslovenski ovčarski pas – šarplaninac (JOP) je pastirski pas sa prostora Zapadnog Balkana na kojem je prisutan od davnina, iako je kao zasebna rasa prepoznat od strane Međunarodne kinološke federacije (FCI) tek 1957. godine. Međutim, podaci o veličini i poreklu osnivačke populacije JOP još uvek su nepoznati. U cilju doprinosa boljem razumevanju genetičke strukture i osnivačke populacije ove rase, izvršili smo dodatnu analizu prethodno objavljenih genetičkih profila 94 jedinke pasa rase JOP (90 pasa koji nisu u srodstvu i dva para punih srodnika uzorkovanih na tri izložbe pasa i u vojnom centru za obuku pasa) utvrđenih na osnovu varijabilnosti devet jedarnih mikrosatelita. Rezultati našeg istraživanja su potvrdili prethodne navode koji se odnose na visok stepen genetičkog diverziteta u ispitivanoj populaciji JOP (HE=0.728±0.027) i nizak stepen ukrštanja u srodstvu, ali i pokazali moguću genetičku strukturu ove rase. Naime, primenom Bajesove metode grupisanja, kao i na osnovu grupisanja po metodi najbližih suseda, nalaza o suvišku homozigota (tzv. Valundov efekat), testova neravnoteže vezanosti gena, analize glavnih komponenti i dvodimenzionalnog skaliranja, ustanovili smo postojanje dve različite genetičke grupe. Jedinke iz obe genetičke grupe bile su približno jednako zastupljene na svim mestima uzorkovanja. Jedna grupa obuhvatala je jedinke sa visokim genetičkim integritetom, dok su drugu grupu činile jedinke hibridnih genetičkih profila, koji su mogli nastali ukrštanjem sa neregistovanim psima koji su u tipu JOP ili sa drugim rasama, kao što je kavkaski ovčar, i/ili sa hibridima nastalih ukrštanjem sa vukovima. Sumirano, ovim istraživanjem ustanovljeno je veoma složeno i raznoliko poreklo JOP, što ukazuje na genetički heterogen karakter osnivačke populacije ove rase.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Genetička karakterizacija jugoslovenskog ovčarskog psa - šarplaninca, pastirskog psa čuvara sa Zapadnog Balkana
T1  - Genetic characterization of the yugoslavian shepherd dog-sharplanina, a livestock guard dog from the western balkans
VL  - 70
IS  - 3
SP  - 329
EP  - 345
DO  - 10.2478/acve-2020-0025
ER  - 

@article{
author = "Dimitrijević, Vladimir and Savić, Mila and Tarić, Elmin and Stanišić, Ljubodrag and Stanimirović, Zoran and Tabaković, Aleksandar and Aleksić, M. Jelena",
year = "2020",
abstract = "Yugoslavian Shepherd Dog-Sharplanina (YSD) is a livestock guard dog from the Western Balkans present in this region over a long time, but recognized by the Fédération Cynologique Internationale as a distinct breed as late as 1957. However, the information regarding the origin and the size of the breed's foundation stock is still lacking. In order to contribute towards better understanding of the genetic make-up of the YSD and its foundation stock, we re-analyzed previously generated genetic profiles of 94 registered YSD dogs assessed with nine nuclear microsatellites. Studied individuals comprised 90 unrelated dogs and two pairs of full-sibs, sampled at four sampling sites: Three dog shows and at a military training centre for dogs in Serbia. We supported earlier findings on high levels of genetic diversity in YSD (HE=0.728±0.027) and lack of inbreeding, and revealed substructure of the breed because we found two distinct gene pools in the Bayesian clustering analysis, indicated also by the excess of homozygotes (i.e., Wahlund effect) and outcomes of other analyses: Linkage disequilibrium tests, Neighbour-Joining tree, principal coordinates and two-dimensional scaling analyses. The two gene pools were almost equally represented at each sampling site. One gene pool was composed of individuals with high genetic integrity, while the other gene pool was characterized with admixed ancestry, developed possibly via hybridization with native breeding stock outside the registry system, other breeds, such as the Caucasian Shepherd, and/or individuals admixed with wolves. Thus, we demonstrate rather complex and diverse ancestry implying a genetically heterogeneous foundation stock of the YSD., Jugoslovenski ovčarski pas – šarplaninac (JOP) je pastirski pas sa prostora Zapadnog Balkana na kojem je prisutan od davnina, iako je kao zasebna rasa prepoznat od strane Međunarodne kinološke federacije (FCI) tek 1957. godine. Međutim, podaci o veličini i poreklu osnivačke populacije JOP još uvek su nepoznati. U cilju doprinosa boljem razumevanju genetičke strukture i osnivačke populacije ove rase, izvršili smo dodatnu analizu prethodno objavljenih genetičkih profila 94 jedinke pasa rase JOP (90 pasa koji nisu u srodstvu i dva para punih srodnika uzorkovanih na tri izložbe pasa i u vojnom centru za obuku pasa) utvrđenih na osnovu varijabilnosti devet jedarnih mikrosatelita. Rezultati našeg istraživanja su potvrdili prethodne navode koji se odnose na visok stepen genetičkog diverziteta u ispitivanoj populaciji JOP (HE=0.728±0.027) i nizak stepen ukrštanja u srodstvu, ali i pokazali moguću genetičku strukturu ove rase. Naime, primenom Bajesove metode grupisanja, kao i na osnovu grupisanja po metodi najbližih suseda, nalaza o suvišku homozigota (tzv. Valundov efekat), testova neravnoteže vezanosti gena, analize glavnih komponenti i dvodimenzionalnog skaliranja, ustanovili smo postojanje dve različite genetičke grupe. Jedinke iz obe genetičke grupe bile su približno jednako zastupljene na svim mestima uzorkovanja. Jedna grupa obuhvatala je jedinke sa visokim genetičkim integritetom, dok su drugu grupu činile jedinke hibridnih genetičkih profila, koji su mogli nastali ukrštanjem sa neregistovanim psima koji su u tipu JOP ili sa drugim rasama, kao što je kavkaski ovčar, i/ili sa hibridima nastalih ukrštanjem sa vukovima. Sumirano, ovim istraživanjem ustanovljeno je veoma složeno i raznoliko poreklo JOP, što ukazuje na genetički heterogen karakter osnivačke populacije ove rase.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Genetička karakterizacija jugoslovenskog ovčarskog psa - šarplaninca, pastirskog psa čuvara sa Zapadnog Balkana, Genetic characterization of the yugoslavian shepherd dog-sharplanina, a livestock guard dog from the western balkans",
volume = "70",
number = "3",
pages = "329-345",
doi = "10.2478/acve-2020-0025"
}

Dimitrijević, V., Savić, M., Tarić, E., Stanišić, L., Stanimirović, Z., Tabaković, A.,& Aleksić, M. J.. (2020). Genetička karakterizacija jugoslovenskog ovčarskog psa - šarplaninca, pastirskog psa čuvara sa Zapadnog Balkana. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(3), 329-345.
https://doi.org/10.2478/acve-2020-0025

Dimitrijević V, Savić M, Tarić E, Stanišić L, Stanimirović Z, Tabaković A, Aleksić MJ. Genetička karakterizacija jugoslovenskog ovčarskog psa - šarplaninca, pastirskog psa čuvara sa Zapadnog Balkana. in Acta Veterinaria-Beograd. 2020;70(3):329-345.
doi:10.2478/acve-2020-0025 .

Dimitrijević, Vladimir, Savić, Mila, Tarić, Elmin, Stanišić, Ljubodrag, Stanimirović, Zoran, Tabaković, Aleksandar, Aleksić, M. Jelena, "Genetička karakterizacija jugoslovenskog ovčarskog psa - šarplaninca, pastirskog psa čuvara sa Zapadnog Balkana" in Acta Veterinaria-Beograd, 70, no. 3 (2020):329-345,
https://doi.org/10.2478/acve-2020-0025 . .

TY  - JOUR
AU  - Dimitrijević, Vladimir
AU  - Ristanić, Marko
AU  - Stanišić, Ljubodrag
AU  - Drobnjak, Darko
AU  - Urošević, Milivoje
AU  - Ozkanal, Umit
AU  - Stanimirović, Zoran
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2039
AB  - The Kangal Shepherd Dog is considered the most common dog breed of Turkish origin.
This study investigated variations in ten autosomal microsatellite markers (PEZ01,
PEZ03, PEZ05, PEZ06, PEZ08, PEZ12, PEZ20, FHC2010, FHC2054 and FHC2079)
for the purposes of genetic diversity assessment of the Kangal breed. In addition, the
use of markers was assessed in parentage testing and individual identification within
the Kangal breed. The microsatellite markers were typed in 51 Kangal dogs. The total
number of alleles in the study population was 69. The mean number of alleles per locus
was 6.9, and varied from four (FHC2079) to 12 (PEZ12). The polymorphic information
content (PIC) ranged from 0.52 (FHC2079) to 0.87 (PEZ12), with the mean value
for all loci of 0.717. Power of exclusion (PE) in 10 microsatellites investigated varied
between 0.143 (FHC2054) and 0.472 (PEZ08) per locus. In order to determine the
efficiency of using microsatellites for individual identification in the Kangal breed,
power of discrimination (PD) and matching probability (MP) were calculated for each
microsatellite marker. The panel achieved high combined MP (6.77 × 10-10) and high
combined PD value of 99.99999%. The obtained results may contribute to further
recognition of the Kangal breed, and confirm that the investigated microsatellites
enable a reliable parentage testing and individual identification of the breed.
AB  - Kangal rasa pasa se smatra jednom od najčešćih rasa turskog porekla. Ova studija
je istraživala varijacije deset autozomalnih mikrosatelitskih markera (PEZ01, PEZ03,
PEZ05, PEZ06, PEZ08, PEZ12, PEZ20, FHC2010, FHC2054 i FHC2079) u svrhu
određivanja genetičkog diverziteta pasa rase kangal. Pored toga, izvršena je procena
mogućnosti primene ovih markera u individualnoj identifikaciji i u kontroli roditeljstva pasa rase kangal. Izvršena je tipizacija navedenih mikrosatelitskih markera kod 51
jedinke kangal rase. Ukupan broj alela u ispitivanoj populaciji iznosio je 69. Prosečan
broj alela po lokusu iznosio je 6,9 sa varijacijama od četiri (FHC2079) do 12 (PEZ12).
Polimorfizam (PIC) je varirao od 0,52 (FHC2079) do 0,87 (PEZ12), sa srednjom
vrednošću od 0,717 za sve lokuse. Verovatnoća isključenja (PE) 10 mikrosatelitskih
markera varirala je između 0,143 (FHC2054) i 0,472 (PEZ08) po lokusu. U cilju utvrđivanja efikasnosti u svrhu individualne identifikacije pasa rase kangal, određivana je
moć diskriminacije (PD) kao i verovatnoća podudaranja (MP) za svaki od ispitivanih markera. Ispitivani panel postigao je visoke vrednosti kako kombinovane MP (6,77 ×
10-10) tako i kombinovane PD od 99,99999%. Dobijeni rezultati predstavljaju doprinos
genetičkoj karakterizaciji rase kangal i pokazuju da je ispitivani panel mikrosatelitskih
markera pouzdan u kontroli spornih rodbinskih odnosa i u individualnoj identifikaciji
pasa ove rase.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Use of microsatellites in genetic diversity assessment, parentage testing and individual identification of the Kangal Shepherd Dog
T1  - Upotreba mikrosatelita u proceni genetičkog diverziteta, kontroli roditeljstva i individualnoj identifikaciji pasarase kangal
VL  - 70
IS  - 2
SP  - 170
EP  - 181
DO  - 10.2478/acve-2020-0012
ER  - 

@article{
author = "Dimitrijević, Vladimir and Ristanić, Marko and Stanišić, Ljubodrag and Drobnjak, Darko and Urošević, Milivoje and Ozkanal, Umit and Stanimirović, Zoran",
year = "2020",
abstract = "The Kangal Shepherd Dog is considered the most common dog breed of Turkish origin.
This study investigated variations in ten autosomal microsatellite markers (PEZ01,
PEZ03, PEZ05, PEZ06, PEZ08, PEZ12, PEZ20, FHC2010, FHC2054 and FHC2079)
for the purposes of genetic diversity assessment of the Kangal breed. In addition, the
use of markers was assessed in parentage testing and individual identification within
the Kangal breed. The microsatellite markers were typed in 51 Kangal dogs. The total
number of alleles in the study population was 69. The mean number of alleles per locus
was 6.9, and varied from four (FHC2079) to 12 (PEZ12). The polymorphic information
content (PIC) ranged from 0.52 (FHC2079) to 0.87 (PEZ12), with the mean value
for all loci of 0.717. Power of exclusion (PE) in 10 microsatellites investigated varied
between 0.143 (FHC2054) and 0.472 (PEZ08) per locus. In order to determine the
efficiency of using microsatellites for individual identification in the Kangal breed,
power of discrimination (PD) and matching probability (MP) were calculated for each
microsatellite marker. The panel achieved high combined MP (6.77 × 10-10) and high
combined PD value of 99.99999%. The obtained results may contribute to further
recognition of the Kangal breed, and confirm that the investigated microsatellites
enable a reliable parentage testing and individual identification of the breed., Kangal rasa pasa se smatra jednom od najčešćih rasa turskog porekla. Ova studija
je istraživala varijacije deset autozomalnih mikrosatelitskih markera (PEZ01, PEZ03,
PEZ05, PEZ06, PEZ08, PEZ12, PEZ20, FHC2010, FHC2054 i FHC2079) u svrhu
određivanja genetičkog diverziteta pasa rase kangal. Pored toga, izvršena je procena
mogućnosti primene ovih markera u individualnoj identifikaciji i u kontroli roditeljstva pasa rase kangal. Izvršena je tipizacija navedenih mikrosatelitskih markera kod 51
jedinke kangal rase. Ukupan broj alela u ispitivanoj populaciji iznosio je 69. Prosečan
broj alela po lokusu iznosio je 6,9 sa varijacijama od četiri (FHC2079) do 12 (PEZ12).
Polimorfizam (PIC) je varirao od 0,52 (FHC2079) do 0,87 (PEZ12), sa srednjom
vrednošću od 0,717 za sve lokuse. Verovatnoća isključenja (PE) 10 mikrosatelitskih
markera varirala je između 0,143 (FHC2054) i 0,472 (PEZ08) po lokusu. U cilju utvrđivanja efikasnosti u svrhu individualne identifikacije pasa rase kangal, određivana je
moć diskriminacije (PD) kao i verovatnoća podudaranja (MP) za svaki od ispitivanih markera. Ispitivani panel postigao je visoke vrednosti kako kombinovane MP (6,77 ×
10-10) tako i kombinovane PD od 99,99999%. Dobijeni rezultati predstavljaju doprinos
genetičkoj karakterizaciji rase kangal i pokazuju da je ispitivani panel mikrosatelitskih
markera pouzdan u kontroli spornih rodbinskih odnosa i u individualnoj identifikaciji
pasa ove rase.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Use of microsatellites in genetic diversity assessment, parentage testing and individual identification of the Kangal Shepherd Dog, Upotreba mikrosatelita u proceni genetičkog diverziteta, kontroli roditeljstva i individualnoj identifikaciji pasarase kangal",
volume = "70",
number = "2",
pages = "170-181",
doi = "10.2478/acve-2020-0012"
}

Dimitrijević, V., Ristanić, M., Stanišić, L., Drobnjak, D., Urošević, M., Ozkanal, U.,& Stanimirović, Z.. (2020). Use of microsatellites in genetic diversity assessment, parentage testing and individual identification of the Kangal Shepherd Dog. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(2), 170-181.
https://doi.org/10.2478/acve-2020-0012

Dimitrijević V, Ristanić M, Stanišić L, Drobnjak D, Urošević M, Ozkanal U, Stanimirović Z. Use of microsatellites in genetic diversity assessment, parentage testing and individual identification of the Kangal Shepherd Dog. in Acta Veterinaria-Beograd. 2020;70(2):170-181.
doi:10.2478/acve-2020-0012 .

Dimitrijević, Vladimir, Ristanić, Marko, Stanišić, Ljubodrag, Drobnjak, Darko, Urošević, Milivoje, Ozkanal, Umit, Stanimirović, Zoran, "Use of microsatellites in genetic diversity assessment, parentage testing and individual identification of the Kangal Shepherd Dog" in Acta Veterinaria-Beograd, 70, no. 2 (2020):170-181,
https://doi.org/10.2478/acve-2020-0012 . .

TY  - CONF
AU  - Aleksić, Jelena
AU  - Kirovski, Danijela
AU  - Stojanac, Nenad
AU  - Nedić, Svetlana
AU  - Vakanjac, Slobodanka
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3809
AB  - U radu je dat pregled zakonodavstva Republike Srbije iz oblasti animalne reprodukcije, kao i zakonodavstva Evropske Unije iz oblasti reprodukcije, embriotransfera, uslova koje treba da ispune timovi za manipulaciju embrionima i uslova
za izvoz embriona iz trećih zemalja u zemlje članice EU. Ukazano na neophodnost donošenja normativnih akta kojima bi se definisali uslovi za postojanje i rad
laboratorija u pogledu objekata (prostorija), opreme, sredstava za rad i stručnog
kadra u kojima bi se obavljala manipulacija embrionima i jajnim ćelijama. Na ovaj
način će znanja i veštine specijalista animalne biotehnologije u reprodukciji, stečena tokom studija na akreditovanim specijalističkim studijama na Katedri za porodiljstvo, reprodukciju i veštačko osemenjavanje Fakulteta veterinarske medicine
Univerziteta u Beogradu biti na pravi način iskorišćena u razvoju animalne biotehnologije u veterinarskoj medicini u Srbiji.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020
T1  - Savremene tehnike u animalnoj reprodukciji u svetlu važećih propisa
SP  - 3
EP  - 12
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3809
ER  - 

@conference{
author = "Aleksić, Jelena and Kirovski, Danijela and Stojanac, Nenad and Nedić, Svetlana and Vakanjac, Slobodanka",
year = "2020",
abstract = "U radu je dat pregled zakonodavstva Republike Srbije iz oblasti animalne reprodukcije, kao i zakonodavstva Evropske Unije iz oblasti reprodukcije, embriotransfera, uslova koje treba da ispune timovi za manipulaciju embrionima i uslova
za izvoz embriona iz trećih zemalja u zemlje članice EU. Ukazano na neophodnost donošenja normativnih akta kojima bi se definisali uslovi za postojanje i rad
laboratorija u pogledu objekata (prostorija), opreme, sredstava za rad i stručnog
kadra u kojima bi se obavljala manipulacija embrionima i jajnim ćelijama. Na ovaj
način će znanja i veštine specijalista animalne biotehnologije u reprodukciji, stečena tokom studija na akreditovanim specijalističkim studijama na Katedri za porodiljstvo, reprodukciju i veštačko osemenjavanje Fakulteta veterinarske medicine
Univerziteta u Beogradu biti na pravi način iskorišćena u razvoju animalne biotehnologije u veterinarskoj medicini u Srbiji.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020",
title = "Savremene tehnike u animalnoj reprodukciji u svetlu važećih propisa",
pages = "3-12",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3809"
}

Aleksić, J., Kirovski, D., Stojanac, N., Nedić, S.,& Vakanjac, S.. (2020). Savremene tehnike u animalnoj reprodukciji u svetlu važećih propisa. in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 3-12.
https://hdl.handle.net/21.15107/rcub_veterinar_3809

Aleksić J, Kirovski D, Stojanac N, Nedić S, Vakanjac S. Savremene tehnike u animalnoj reprodukciji u svetlu važećih propisa. in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020. 2020;:3-12.
https://hdl.handle.net/21.15107/rcub_veterinar_3809 .

Aleksić, Jelena, Kirovski, Danijela, Stojanac, Nenad, Nedić, Svetlana, Vakanjac, Slobodanka, "Savremene tehnike u animalnoj reprodukciji u svetlu važećih propisa" in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020 (2020):3-12,
https://hdl.handle.net/21.15107/rcub_veterinar_3809 .

TY  - JOUR
AU  - Gray, Alison
AU  - Adjlane, Noureddine
AU  - Arab, Arab
AU  - Ballis, Alexis
AU  - Brusbardis, Valters
AU  - Charrière, Jean-Daniel
AU  - Chlebo, Robert
AU  - Coffey, Mary F.
AU  - Cornelissen, Bram
AU  - Amaro da Costa, Cristina
AU  - Dahle, Bjørn
AU  - Danihlík, Jiří
AU  - Dražić, Marica Maja
AU  - Evans, Garth
AU  - Fedoriak, Mariia
AU  - Forsythe, Ivan
AU  - Gajda, Anna
AU  - de Graaf, Dirk C.
AU  - Gregorc, Aleš
AU  - Ilieva, Iliyana
AU  - Johannesen, Jes
AU  - Kauko, Lassi
AU  - Kristiansen, Preben
AU  - Martikkala, Maritta
AU  - Martín-Hernández, Raquel
AU  - Medina-Flores, Carlos Aurelio
AU  - Mutinelli, Franco
AU  - Patalano, Solenn
AU  - Raudmets, Aivar
AU  - Martin, Gilles San
AU  - Soroker, Victoria
AU  - Stevanović, Jevrosima
AU  - Uzunov, Aleksandar
AU  - Vejsnaes, Flemming
AU  - Williams, Anthony
AU  - Zammit-Mangion, Marion
AU  - Brodschneider, Robert
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1983
AB  - This article presents managed honey bee colony loss rates over winter 2018/19 resulting from using the standardised COLOSS questionnaire in 35 countries (31 in Europe). In total, 28,629 beekeepers supplying valid loss data wintered 738,233 colonies, and reported 29,912 (4.1%, 95% confidence interval (CI) 4.0–4.1%) colonies with unsolvable queen problems, 79,146 (10.7%, 95% CI 10.5–10.9%) dead colonies after winter and 13,895 colonies (1.9%, 95% CI 1.8–2.0%) lost through natural disaster. This gave an overall colony winter loss rate of 16.7% (95% CI 16.4–16.9%), varying greatly between countries, from 5.8% to 32.0%. We modelled the risk of loss as a dead/empty colony or from unresolvable queen problems, and found that, overall, larger beekeeping operations with more than 150 colonies experienced significantly lower losses (p ' 0.001), consistent with earlier studies. Additionally, beekeepers included in this survey who did not migrate their colonies at least once in 2018 had significantly lower losses than those migrating (p ' 0.001). The percentage of new queens from 2018 in wintered colonies was also examined as a potential risk factor. The percentage of colonies going into winter with a new queen was estimated as 55.0% over all countries. Higher percentages of young queens corresponded to lower overall losses (excluding losses from natural disaster), but also lower losses from unresolvable queen problems, and lower losses from winter mortality (p ' 0.001). Detailed results for each country and overall are given in a table, and a map shows relative risks of winter loss at regional level.
PB  - Taylor and Francis Ltd.
T2  - Journal of Apicultural Research
T1  - Honey bee colony winter loss rates for 35 countries participating in the COLOSS survey for winter 2018–2019, and the effects of a new queen on the risk of colony winter loss
VL  - 59
IS  - 5
SP  - 744
EP  - 751
DO  - 10.1080/00218839.2020.1797272
ER  - 

@article{
author = "Gray, Alison and Adjlane, Noureddine and Arab, Arab and Ballis, Alexis and Brusbardis, Valters and Charrière, Jean-Daniel and Chlebo, Robert and Coffey, Mary F. and Cornelissen, Bram and Amaro da Costa, Cristina and Dahle, Bjørn and Danihlík, Jiří and Dražić, Marica Maja and Evans, Garth and Fedoriak, Mariia and Forsythe, Ivan and Gajda, Anna and de Graaf, Dirk C. and Gregorc, Aleš and Ilieva, Iliyana and Johannesen, Jes and Kauko, Lassi and Kristiansen, Preben and Martikkala, Maritta and Martín-Hernández, Raquel and Medina-Flores, Carlos Aurelio and Mutinelli, Franco and Patalano, Solenn and Raudmets, Aivar and Martin, Gilles San and Soroker, Victoria and Stevanović, Jevrosima and Uzunov, Aleksandar and Vejsnaes, Flemming and Williams, Anthony and Zammit-Mangion, Marion and Brodschneider, Robert",
year = "2020",
abstract = "This article presents managed honey bee colony loss rates over winter 2018/19 resulting from using the standardised COLOSS questionnaire in 35 countries (31 in Europe). In total, 28,629 beekeepers supplying valid loss data wintered 738,233 colonies, and reported 29,912 (4.1%, 95% confidence interval (CI) 4.0–4.1%) colonies with unsolvable queen problems, 79,146 (10.7%, 95% CI 10.5–10.9%) dead colonies after winter and 13,895 colonies (1.9%, 95% CI 1.8–2.0%) lost through natural disaster. This gave an overall colony winter loss rate of 16.7% (95% CI 16.4–16.9%), varying greatly between countries, from 5.8% to 32.0%. We modelled the risk of loss as a dead/empty colony or from unresolvable queen problems, and found that, overall, larger beekeeping operations with more than 150 colonies experienced significantly lower losses (p ' 0.001), consistent with earlier studies. Additionally, beekeepers included in this survey who did not migrate their colonies at least once in 2018 had significantly lower losses than those migrating (p ' 0.001). The percentage of new queens from 2018 in wintered colonies was also examined as a potential risk factor. The percentage of colonies going into winter with a new queen was estimated as 55.0% over all countries. Higher percentages of young queens corresponded to lower overall losses (excluding losses from natural disaster), but also lower losses from unresolvable queen problems, and lower losses from winter mortality (p ' 0.001). Detailed results for each country and overall are given in a table, and a map shows relative risks of winter loss at regional level.",
publisher = "Taylor and Francis Ltd.",
journal = "Journal of Apicultural Research",
title = "Honey bee colony winter loss rates for 35 countries participating in the COLOSS survey for winter 2018–2019, and the effects of a new queen on the risk of colony winter loss",
volume = "59",
number = "5",
pages = "744-751",
doi = "10.1080/00218839.2020.1797272"
}

Gray, A., Adjlane, N., Arab, A., Ballis, A., Brusbardis, V., Charrière, J., Chlebo, R., Coffey, M. F., Cornelissen, B., Amaro da Costa, C., Dahle, B., Danihlík, J., Dražić, M. M., Evans, G., Fedoriak, M., Forsythe, I., Gajda, A., de Graaf, D. C., Gregorc, A., Ilieva, I., Johannesen, J., Kauko, L., Kristiansen, P., Martikkala, M., Martín-Hernández, R., Medina-Flores, C. A., Mutinelli, F., Patalano, S., Raudmets, A., Martin, G. S., Soroker, V., Stevanović, J., Uzunov, A., Vejsnaes, F., Williams, A., Zammit-Mangion, M.,& Brodschneider, R.. (2020). Honey bee colony winter loss rates for 35 countries participating in the COLOSS survey for winter 2018–2019, and the effects of a new queen on the risk of colony winter loss. in Journal of Apicultural Research
Taylor and Francis Ltd.., 59(5), 744-751.
https://doi.org/10.1080/00218839.2020.1797272

Gray A, Adjlane N, Arab A, Ballis A, Brusbardis V, Charrière J, Chlebo R, Coffey MF, Cornelissen B, Amaro da Costa C, Dahle B, Danihlík J, Dražić MM, Evans G, Fedoriak M, Forsythe I, Gajda A, de Graaf DC, Gregorc A, Ilieva I, Johannesen J, Kauko L, Kristiansen P, Martikkala M, Martín-Hernández R, Medina-Flores CA, Mutinelli F, Patalano S, Raudmets A, Martin GS, Soroker V, Stevanović J, Uzunov A, Vejsnaes F, Williams A, Zammit-Mangion M, Brodschneider R. Honey bee colony winter loss rates for 35 countries participating in the COLOSS survey for winter 2018–2019, and the effects of a new queen on the risk of colony winter loss. in Journal of Apicultural Research. 2020;59(5):744-751.
doi:10.1080/00218839.2020.1797272 .

Gray, Alison, Adjlane, Noureddine, Arab, Arab, Ballis, Alexis, Brusbardis, Valters, Charrière, Jean-Daniel, Chlebo, Robert, Coffey, Mary F., Cornelissen, Bram, Amaro da Costa, Cristina, Dahle, Bjørn, Danihlík, Jiří, Dražić, Marica Maja, Evans, Garth, Fedoriak, Mariia, Forsythe, Ivan, Gajda, Anna, de Graaf, Dirk C., Gregorc, Aleš, Ilieva, Iliyana, Johannesen, Jes, Kauko, Lassi, Kristiansen, Preben, Martikkala, Maritta, Martín-Hernández, Raquel, Medina-Flores, Carlos Aurelio, Mutinelli, Franco, Patalano, Solenn, Raudmets, Aivar, Martin, Gilles San, Soroker, Victoria, Stevanović, Jevrosima, Uzunov, Aleksandar, Vejsnaes, Flemming, Williams, Anthony, Zammit-Mangion, Marion, Brodschneider, Robert, "Honey bee colony winter loss rates for 35 countries participating in the COLOSS survey for winter 2018–2019, and the effects of a new queen on the risk of colony winter loss" in Journal of Apicultural Research, 59, no. 5 (2020):744-751,
https://doi.org/10.1080/00218839.2020.1797272 . .

TY  - CONF
AU  - Vujanac, Ivan
AU  - Prodanović, Radiša
AU  - Nedić, Sreten
AU  - Arsić, Sveta
AU  - Jovanović, Ljubomir
AU  - Kirovski, Danijela
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3810
AB  - Metabolički profil predstavlja merenje pojedinih biohemijskih parametara krvi koji su relevantni za procenu zdravstvenog statusa i proizvodnih sposobnosti visokomlečnih krava. Poseban značaj metaboličkog profila se ogleda u proceni i kontroli iskoristivosti hranljivih materija iz obroka kod različitih proizvodnih kategorija. Pored toga, on u
farmskim uslovima omogućava pravovremeno otkrivanje poremećaja u funkciji pojedinih organa i/ili sistema organa, pogotovo kada su
u pitanju poremećaji zdravlja koji se manifestuju nižom proizvodnjom
mleka, smanjenom plodnošću i dugim servis periodom na nivou zapata. Za rutinsko određivanje parametara metaboličkog profila, u krvnom serumu krava se mere: koncentracija glukoze, ukupnih proteina,
albumina, uree, kalcijuma, neorganskog fosfora, magnezijuma, ukupnog bilirubina, holesterola, triglicerida, beta hidroksi butirata (BHB),
slobodnih masnih kiselina (NEFA) i aktivnost enzima za procenu funkcionalnog stanja jetre (AST i GGT). Parametri koje treba odrediti za
dobijanje metaboličkog profila mogu biti različiti i utvrđuju se na osnovu zdravstvene, proizvodne i reproduktivne problematike pojedinih zapata. Svakako da odabir parametara određuje i poznavanje etiologije
i patogeneze oboljenja koja mogu da se ispoljavaju u supkliničkoj i kliničkoj formi. Ispitivanjem treba obuhvatiti krave tokom svih faza proizvodno – reproduktivnog ciklusa, zasušenja, graviditeta, puerperijuma
i laktacije. Za pravilno tumačenje izmerenih vrednosti metaboličkog
profila neophodno je prethodno uzeti anamnestičke podatke o stadu,
obaviti klinički pregled, proceniti uslove smeštaja i nege, odrediti telesnu kondiciju životinja, kvalitet hraniva, sastav obroka i režim ishrane.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLI Seminar za inovacije znanja veterinara, Beograd, 2020
T1  - Upotreba metaboličkog profila u kontroli zdravlja i proizvodnih osobina visokomlečnih krava
SP  - 133
EP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3810
ER  - 

@conference{
author = "Vujanac, Ivan and Prodanović, Radiša and Nedić, Sreten and Arsić, Sveta and Jovanović, Ljubomir and Kirovski, Danijela",
year = "2020",
abstract = "Metabolički profil predstavlja merenje pojedinih biohemijskih parametara krvi koji su relevantni za procenu zdravstvenog statusa i proizvodnih sposobnosti visokomlečnih krava. Poseban značaj metaboličkog profila se ogleda u proceni i kontroli iskoristivosti hranljivih materija iz obroka kod različitih proizvodnih kategorija. Pored toga, on u
farmskim uslovima omogućava pravovremeno otkrivanje poremećaja u funkciji pojedinih organa i/ili sistema organa, pogotovo kada su
u pitanju poremećaji zdravlja koji se manifestuju nižom proizvodnjom
mleka, smanjenom plodnošću i dugim servis periodom na nivou zapata. Za rutinsko određivanje parametara metaboličkog profila, u krvnom serumu krava se mere: koncentracija glukoze, ukupnih proteina,
albumina, uree, kalcijuma, neorganskog fosfora, magnezijuma, ukupnog bilirubina, holesterola, triglicerida, beta hidroksi butirata (BHB),
slobodnih masnih kiselina (NEFA) i aktivnost enzima za procenu funkcionalnog stanja jetre (AST i GGT). Parametri koje treba odrediti za
dobijanje metaboličkog profila mogu biti različiti i utvrđuju se na osnovu zdravstvene, proizvodne i reproduktivne problematike pojedinih zapata. Svakako da odabir parametara određuje i poznavanje etiologije
i patogeneze oboljenja koja mogu da se ispoljavaju u supkliničkoj i kliničkoj formi. Ispitivanjem treba obuhvatiti krave tokom svih faza proizvodno – reproduktivnog ciklusa, zasušenja, graviditeta, puerperijuma
i laktacije. Za pravilno tumačenje izmerenih vrednosti metaboličkog
profila neophodno je prethodno uzeti anamnestičke podatke o stadu,
obaviti klinički pregled, proceniti uslove smeštaja i nege, odrediti telesnu kondiciju životinja, kvalitet hraniva, sastav obroka i režim ishrane.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLI Seminar za inovacije znanja veterinara, Beograd, 2020",
title = "Upotreba metaboličkog profila u kontroli zdravlja i proizvodnih osobina visokomlečnih krava",
pages = "133-141",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3810"
}

Vujanac, I., Prodanović, R., Nedić, S., Arsić, S., Jovanović, L.,& Kirovski, D.. (2020). Upotreba metaboličkog profila u kontroli zdravlja i proizvodnih osobina visokomlečnih krava. in XLI Seminar za inovacije znanja veterinara, Beograd, 2020
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 133-141.
https://hdl.handle.net/21.15107/rcub_veterinar_3810

Vujanac I, Prodanović R, Nedić S, Arsić S, Jovanović L, Kirovski D. Upotreba metaboličkog profila u kontroli zdravlja i proizvodnih osobina visokomlečnih krava. in XLI Seminar za inovacije znanja veterinara, Beograd, 2020. 2020;:133-141.
https://hdl.handle.net/21.15107/rcub_veterinar_3810 .

Vujanac, Ivan, Prodanović, Radiša, Nedić, Sreten, Arsić, Sveta, Jovanović, Ljubomir, Kirovski, Danijela, "Upotreba metaboličkog profila u kontroli zdravlja i proizvodnih osobina visokomlečnih krava" in XLI Seminar za inovacije znanja veterinara, Beograd, 2020 (2020):133-141,
https://hdl.handle.net/21.15107/rcub_veterinar_3810 .