TY  - JOUR
AU  - Blagojević, Miloš
AU  - Nešić, Ivana
AU  - Đelić, Ninoslav
AU  - Jović, Slavoljub
AU  - Đorđević, Milena
AU  - Savić-Stevanović, Vera
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1056
AB  - The common iliac arteries (a. iliaca communis dextra et a. iliaca communis sinistra) are the terminal branches of the abdominal aorta. The paired external iliac artery (a. iliaca externa dextra et a. iliaca externa sinistra) is a stronger branch of the paired common iliac artery. Before they leave the abdominal cavity and continue as the femoral arteries (a. femoralis dextra et a. femoralis sinistra) each external iliac artery forms the following branches: 1. The internal pudendal artery (a. pudenda interna) which, with its branches, supplies the tensor fasciae latae and quadriceps muscles (a. circumflexa femoris lateralis), cranial region of the urinary bladder, cranial end of the uterine horn in females, the deferent duct and tail of the epididymis in males (a. umbilicalis) the rectum, the external anal sphincter and perineum (a. rectalis caudalis) with blood. 2. The obturator artery (a. obturatoria), which supplies both of the obturator muscles, as well as the proximal end of the adductor muscle. 3. The pudendoepigastric trunk (truncus pudendoepigastricus) is a short vessel, which with its branches (a. pudenda externa, a. epigastrica caudalis et a. urethrogenitalis) supplies the caudal end of the rectus abdominis muscle, tip of the penis, preputium, superficial inguinal lymph nodes, scrotum in males and inguinal mamma in females, transversus and internus abdominis muscles, neck of the urinary bladder, urethra, vagina and vulva in females and accessory glands in males. 4. Paired internal iliac arteries (a. iliaca interna dextra et a. iliaca interna sinistra) are thinner branches than the external iliac arteries. The internal iliac artery with its branches supplies the cranial and caudal parts of the gluteal muscles (a. glutea cranialis et a. glutea caudalis), as well as the muscles of the lateral side of the tail (a. caudalis lateralis).
AB  - Aa. iliacae communes (a. iliaca communis dextra et a. iliaca communis sinistra) su završne grane trbušne aorte. Aa. iliacae externae (a. iliaca externa dextra et a. iliaca externa sinistra) su jače grane od aa. iliacae communes. A. iliaca dextra i a. iliaca sinistra pre nego što napuste trbušnu duplju i nastave kao a. femoralis dextra i a. femoralis sinistra formiraju sledeće grane: 1. A. pudenda interna, sa svojim granama, snabdeva krvlju m. tensor fasciae latae i m. quadriceps femoris (a. circumflexa femoris lateralis), kranijalni deo mokraćne bešike, kranijalni kraj materičnog roga u ženki, semenovod i rep pasemenika u mužjaka (a. umbilicalis), rectum, m. sphincter ani externus i međicu (a. rectalis caudalis). 2. A. obturatoria snabdeva krvlju m. obturator externus, m. obturator internus kao i proksimalni kraj m. adductor-a. 3. Truncus pudendoepigastricus je kratak krvni sud koji sa svojim granama snabdeva krvlju kaudalni kraj m. rectus abdominis-a, vrh penisa, prepucijum, lymphonodi inguinales superficiales, mošnice u mužjaka i ingvinalni deo mlečne žlezde u ženki (a. pudenda externa), paran m. transversus abdominis i paran m. obliquus internus abdominis, (a. epigastrica caudalis), vrat mokraćne bešike, mokraćni izvodnik, vaginu i vulvu u ženki i akcesorne polne žlezde u mužjaka (a. urethrogenitalis). 4. Aa. iliacae internae (a. iliaca interna dextra et a. iliaca interna sinistra) su slabije grane od aa. iliacae communes. Aa. iliacae internae, svojim granama snabdevaju krvlju glutealne mišiće (a. glutea cranialis i a. glutea caudalis), kao i mišiće na lateralnoj strani repa (a. caudalis lateralis).
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The common iliac artery in the ground squirrel (Citellus citellus)
T1  - Arterija iliaca communis u tekunice (Citellus citellus)
VL  - 63
IS  - 4
SP  - 463
EP  - 470
DO  - 10.2298/AVB1304463B
ER  - 

@article{
author = "Blagojević, Miloš and Nešić, Ivana and Đelić, Ninoslav and Jović, Slavoljub and Đorđević, Milena and Savić-Stevanović, Vera",
year = "2013",
abstract = "The common iliac arteries (a. iliaca communis dextra et a. iliaca communis sinistra) are the terminal branches of the abdominal aorta. The paired external iliac artery (a. iliaca externa dextra et a. iliaca externa sinistra) is a stronger branch of the paired common iliac artery. Before they leave the abdominal cavity and continue as the femoral arteries (a. femoralis dextra et a. femoralis sinistra) each external iliac artery forms the following branches: 1. The internal pudendal artery (a. pudenda interna) which, with its branches, supplies the tensor fasciae latae and quadriceps muscles (a. circumflexa femoris lateralis), cranial region of the urinary bladder, cranial end of the uterine horn in females, the deferent duct and tail of the epididymis in males (a. umbilicalis) the rectum, the external anal sphincter and perineum (a. rectalis caudalis) with blood. 2. The obturator artery (a. obturatoria), which supplies both of the obturator muscles, as well as the proximal end of the adductor muscle. 3. The pudendoepigastric trunk (truncus pudendoepigastricus) is a short vessel, which with its branches (a. pudenda externa, a. epigastrica caudalis et a. urethrogenitalis) supplies the caudal end of the rectus abdominis muscle, tip of the penis, preputium, superficial inguinal lymph nodes, scrotum in males and inguinal mamma in females, transversus and internus abdominis muscles, neck of the urinary bladder, urethra, vagina and vulva in females and accessory glands in males. 4. Paired internal iliac arteries (a. iliaca interna dextra et a. iliaca interna sinistra) are thinner branches than the external iliac arteries. The internal iliac artery with its branches supplies the cranial and caudal parts of the gluteal muscles (a. glutea cranialis et a. glutea caudalis), as well as the muscles of the lateral side of the tail (a. caudalis lateralis)., Aa. iliacae communes (a. iliaca communis dextra et a. iliaca communis sinistra) su završne grane trbušne aorte. Aa. iliacae externae (a. iliaca externa dextra et a. iliaca externa sinistra) su jače grane od aa. iliacae communes. A. iliaca dextra i a. iliaca sinistra pre nego što napuste trbušnu duplju i nastave kao a. femoralis dextra i a. femoralis sinistra formiraju sledeće grane: 1. A. pudenda interna, sa svojim granama, snabdeva krvlju m. tensor fasciae latae i m. quadriceps femoris (a. circumflexa femoris lateralis), kranijalni deo mokraćne bešike, kranijalni kraj materičnog roga u ženki, semenovod i rep pasemenika u mužjaka (a. umbilicalis), rectum, m. sphincter ani externus i međicu (a. rectalis caudalis). 2. A. obturatoria snabdeva krvlju m. obturator externus, m. obturator internus kao i proksimalni kraj m. adductor-a. 3. Truncus pudendoepigastricus je kratak krvni sud koji sa svojim granama snabdeva krvlju kaudalni kraj m. rectus abdominis-a, vrh penisa, prepucijum, lymphonodi inguinales superficiales, mošnice u mužjaka i ingvinalni deo mlečne žlezde u ženki (a. pudenda externa), paran m. transversus abdominis i paran m. obliquus internus abdominis, (a. epigastrica caudalis), vrat mokraćne bešike, mokraćni izvodnik, vaginu i vulvu u ženki i akcesorne polne žlezde u mužjaka (a. urethrogenitalis). 4. Aa. iliacae internae (a. iliaca interna dextra et a. iliaca interna sinistra) su slabije grane od aa. iliacae communes. Aa. iliacae internae, svojim granama snabdevaju krvlju glutealne mišiće (a. glutea cranialis i a. glutea caudalis), kao i mišiće na lateralnoj strani repa (a. caudalis lateralis).",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The common iliac artery in the ground squirrel (Citellus citellus), Arterija iliaca communis u tekunice (Citellus citellus)",
volume = "63",
number = "4",
pages = "463-470",
doi = "10.2298/AVB1304463B"
}

Blagojević, M., Nešić, I., Đelić, N., Jović, S., Đorđević, M.,& Savić-Stevanović, V.. (2013). The common iliac artery in the ground squirrel (Citellus citellus). in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(4), 463-470.
https://doi.org/10.2298/AVB1304463B

Blagojević M, Nešić I, Đelić N, Jović S, Đorđević M, Savić-Stevanović V. The common iliac artery in the ground squirrel (Citellus citellus). in Acta Veterinaria-Beograd. 2013;63(4):463-470.
doi:10.2298/AVB1304463B .

Blagojević, Miloš, Nešić, Ivana, Đelić, Ninoslav, Jović, Slavoljub, Đorđević, Milena, Savić-Stevanović, Vera, "The common iliac artery in the ground squirrel (Citellus citellus)" in Acta Veterinaria-Beograd, 63, no. 4 (2013):463-470,
https://doi.org/10.2298/AVB1304463B . .

TY  - JOUR
AU  - Krstić, Milena
AU  - Sovilj, Sofija P.
AU  - Grgurić-Šipka, Sanja
AU  - Radosavljevic-Evans, Ivana
AU  - Borozan, Sunčica
AU  - Santibanez, Juan Francisco
AU  - Kocić, Jelena
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/710
AB  - Three new complexes of the general formula L[RuCl3(DMSO)(3)] (1-3), where L = chlorpromazine hydrochloride, trifluoroperazine dihydrochloride or thioridazine hydrochloride, were prepared and characterized by elemental analysis and spectroscopic methods (FT-IR, UV-Vis, H-1 NMR and C-13 NMR). In addition, the crystal structure of the complex 2 containing trifluoroperazine dihydrochloride was solved by single crystal X-ray diffraction. The complex crystallizes in the monoclinic system, space group P2(1)/n, with a = 10.4935(7) angstrom, b = 18.6836(12) angstrom, c = 19.9250(13) angstrom, beta = 98.448(2)degrees, V = 3864.0(4) angstrom(3). The structure was refined to the agreement factors of R = 4.79%, R-w = 11.23%. The effect of three different doses (0.4, 4.5 and 90.4 mu M/kg bw) of complex 2 on superoxide dismutase (SOD) and catalase (CAT) activity was investigated under physiological conditions. Influence on nitrite production (NO2-) and the level of erythrocytes malondialdehyde (MDA) in rats blood was also evaluated. Complex 2 did not affect the CAT enzyme activity in vivo and did not cause the hydroxyl radicals production. In the 0.4 and 4.5 mu M/kg bw doses it showed almost the same or lower SOD activity and nitrite levels, while the dose of 90.4 mu M/kg bw significantly increased these parameters. Finally, the cytotoxicity of complexes were assayed in four human carcinoma cell lines MCF-7, MDA-MB-453 (breast carcinoma), SW-480 (colon adenocarcinoma) and IM9 (myeloma multiple cells). Antiproliferative activity in vitro with low IC50 during 48 h of treatment was observed.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - European Journal of Medicinal Chemistry
T1  - New ruthenium(II) complexes with N-alkylphenothiazines: Synthesis, structure, in vivo activity as free radical scavengers and in vitro cytotoxicity
VL  - 45
IS  - 9
SP  - 3669
EP  - 3676
DO  - 10.1016/j.ejmech.2010.05.013
ER  - 

@article{
author = "Krstić, Milena and Sovilj, Sofija P. and Grgurić-Šipka, Sanja and Radosavljevic-Evans, Ivana and Borozan, Sunčica and Santibanez, Juan Francisco and Kocić, Jelena",
year = "2010",
abstract = "Three new complexes of the general formula L[RuCl3(DMSO)(3)] (1-3), where L = chlorpromazine hydrochloride, trifluoroperazine dihydrochloride or thioridazine hydrochloride, were prepared and characterized by elemental analysis and spectroscopic methods (FT-IR, UV-Vis, H-1 NMR and C-13 NMR). In addition, the crystal structure of the complex 2 containing trifluoroperazine dihydrochloride was solved by single crystal X-ray diffraction. The complex crystallizes in the monoclinic system, space group P2(1)/n, with a = 10.4935(7) angstrom, b = 18.6836(12) angstrom, c = 19.9250(13) angstrom, beta = 98.448(2)degrees, V = 3864.0(4) angstrom(3). The structure was refined to the agreement factors of R = 4.79%, R-w = 11.23%. The effect of three different doses (0.4, 4.5 and 90.4 mu M/kg bw) of complex 2 on superoxide dismutase (SOD) and catalase (CAT) activity was investigated under physiological conditions. Influence on nitrite production (NO2-) and the level of erythrocytes malondialdehyde (MDA) in rats blood was also evaluated. Complex 2 did not affect the CAT enzyme activity in vivo and did not cause the hydroxyl radicals production. In the 0.4 and 4.5 mu M/kg bw doses it showed almost the same or lower SOD activity and nitrite levels, while the dose of 90.4 mu M/kg bw significantly increased these parameters. Finally, the cytotoxicity of complexes were assayed in four human carcinoma cell lines MCF-7, MDA-MB-453 (breast carcinoma), SW-480 (colon adenocarcinoma) and IM9 (myeloma multiple cells). Antiproliferative activity in vitro with low IC50 during 48 h of treatment was observed.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "European Journal of Medicinal Chemistry",
title = "New ruthenium(II) complexes with N-alkylphenothiazines: Synthesis, structure, in vivo activity as free radical scavengers and in vitro cytotoxicity",
volume = "45",
number = "9",
pages = "3669-3676",
doi = "10.1016/j.ejmech.2010.05.013"
}

Krstić, M., Sovilj, S. P., Grgurić-Šipka, S., Radosavljevic-Evans, I., Borozan, S., Santibanez, J. F.,& Kocić, J.. (2010). New ruthenium(II) complexes with N-alkylphenothiazines: Synthesis, structure, in vivo activity as free radical scavengers and in vitro cytotoxicity. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 45(9), 3669-3676.
https://doi.org/10.1016/j.ejmech.2010.05.013

Krstić M, Sovilj SP, Grgurić-Šipka S, Radosavljevic-Evans I, Borozan S, Santibanez JF, Kocić J. New ruthenium(II) complexes with N-alkylphenothiazines: Synthesis, structure, in vivo activity as free radical scavengers and in vitro cytotoxicity. in European Journal of Medicinal Chemistry. 2010;45(9):3669-3676.
doi:10.1016/j.ejmech.2010.05.013 .

Krstić, Milena, Sovilj, Sofija P., Grgurić-Šipka, Sanja, Radosavljevic-Evans, Ivana, Borozan, Sunčica, Santibanez, Juan Francisco, Kocić, Jelena, "New ruthenium(II) complexes with N-alkylphenothiazines: Synthesis, structure, in vivo activity as free radical scavengers and in vitro cytotoxicity" in European Journal of Medicinal Chemistry, 45, no. 9 (2010):3669-3676,
https://doi.org/10.1016/j.ejmech.2010.05.013 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Plecas-Solarović, Bosiljka
AU  - Đelić, Ninoslav
AU  - Ocić, Gordana
AU  - Smiljković, Predrag
AU  - Siedlak, Sandra L.
AU  - Smith, Mark A.
AU  - Bajić, Vladan
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/728
AB  - Chromosomal alterations are a feature of both aging and Alzheimers disease (AD). This study examined if premature centromere division (PCD), a chromosomal instability indicator increased in AD, is correlated with aging or, instead, represents a de novo chromosomal alteration due to accelerating aging in AD. PCD in peripheral blood lymphocytes was determined in sporadic AD patients and gender and age-matched unaffected controls. Metaphase nuclei were analyzed for chromosomes showing PCD, X chromosomes with PCD (PCD,X), and acrocentric chromosomes showing PCD. AD patients, regardless of age, demonstrated increased PCD on any chromosome and PCD on acrocentric chromosomes in both genders, whereas an increase in frequency of PCD,X was expressed only in women. This cytogenetic analysis suggests that PCD is a feature of AD, rather than an epiphenomenon of chronological aging, and may be useful as a physiological biomarker that can be used for disease diagnosis.
PB  - Oxford Univ Press Inc, Cary
T2  - Journals of Gerontology Series A-Biological Sciences and Medical Sciences
T1  - Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer s Disease Patients: Relation to Gender and Age
VL  - 65
IS  - 12
SP  - 1269
EP  - 1274
DO  - 10.1093/gerona/glq148
ER  - 

@article{
author = "Živković, Lada and Spremo-Potparević, Biljana and Plecas-Solarović, Bosiljka and Đelić, Ninoslav and Ocić, Gordana and Smiljković, Predrag and Siedlak, Sandra L. and Smith, Mark A. and Bajić, Vladan",
year = "2010",
abstract = "Chromosomal alterations are a feature of both aging and Alzheimers disease (AD). This study examined if premature centromere division (PCD), a chromosomal instability indicator increased in AD, is correlated with aging or, instead, represents a de novo chromosomal alteration due to accelerating aging in AD. PCD in peripheral blood lymphocytes was determined in sporadic AD patients and gender and age-matched unaffected controls. Metaphase nuclei were analyzed for chromosomes showing PCD, X chromosomes with PCD (PCD,X), and acrocentric chromosomes showing PCD. AD patients, regardless of age, demonstrated increased PCD on any chromosome and PCD on acrocentric chromosomes in both genders, whereas an increase in frequency of PCD,X was expressed only in women. This cytogenetic analysis suggests that PCD is a feature of AD, rather than an epiphenomenon of chronological aging, and may be useful as a physiological biomarker that can be used for disease diagnosis.",
publisher = "Oxford Univ Press Inc, Cary",
journal = "Journals of Gerontology Series A-Biological Sciences and Medical Sciences",
title = "Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer s Disease Patients: Relation to Gender and Age",
volume = "65",
number = "12",
pages = "1269-1274",
doi = "10.1093/gerona/glq148"
}

Živković, L., Spremo-Potparević, B., Plecas-Solarović, B., Đelić, N., Ocić, G., Smiljković, P., Siedlak, S. L., Smith, M. A.,& Bajić, V.. (2010). Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer s Disease Patients: Relation to Gender and Age. in Journals of Gerontology Series A-Biological Sciences and Medical Sciences
Oxford Univ Press Inc, Cary., 65(12), 1269-1274.
https://doi.org/10.1093/gerona/glq148

Živković L, Spremo-Potparević B, Plecas-Solarović B, Đelić N, Ocić G, Smiljković P, Siedlak SL, Smith MA, Bajić V. Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer s Disease Patients: Relation to Gender and Age. in Journals of Gerontology Series A-Biological Sciences and Medical Sciences. 2010;65(12):1269-1274.
doi:10.1093/gerona/glq148 .

Živković, Lada, Spremo-Potparević, Biljana, Plecas-Solarović, Bosiljka, Đelić, Ninoslav, Ocić, Gordana, Smiljković, Predrag, Siedlak, Sandra L., Smith, Mark A., Bajić, Vladan, "Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer s Disease Patients: Relation to Gender and Age" in Journals of Gerontology Series A-Biological Sciences and Medical Sciences, 65, no. 12 (2010):1269-1274,
https://doi.org/10.1093/gerona/glq148 . .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Spremo-Potparević, Biljana
AU  - Zivković, L.
AU  - Milicević, Z.
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/614
AB  - Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3′,5′ cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assay (CA) on human peripheral blood lymphocytes of healthy individuals and by bone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 μm) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p.) injection of 8-Cl-cAMP in doses of 10,80 and 150 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP. These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes
VL  - 14
IS  - 1
SP  - 71
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_614
ER  - 

@article{
author = "Bajić, Vladan and Stanimirović, Zoran and Stevanović, Jevrosima and Spremo-Potparević, Biljana and Zivković, L. and Milicević, Z.",
year = "2009",
abstract = "Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3′,5′ cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assay (CA) on human peripheral blood lymphocytes of healthy individuals and by bone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 μm) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p.) injection of 8-Cl-cAMP in doses of 10,80 and 150 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP. These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes",
volume = "14",
number = "1",
pages = "71-77",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_614"
}

Bajić, V., Stanimirović, Z., Stevanović, J., Spremo-Potparević, B., Zivković, L.,& Milicević, Z.. (2009). Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 14(1), 71-77.
https://hdl.handle.net/21.15107/rcub_veterinar_614

Bajić V, Stanimirović Z, Stevanović J, Spremo-Potparević B, Zivković L, Milicević Z. Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON. 2009;14(1):71-77.
https://hdl.handle.net/21.15107/rcub_veterinar_614 .

Bajić, Vladan, Stanimirović, Zoran, Stevanović, Jevrosima, Spremo-Potparević, Biljana, Zivković, L., Milicević, Z., "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes" in Journal of BUON, 14, no. 1 (2009):71-77,
https://hdl.handle.net/21.15107/rcub_veterinar_614 .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Đelić, Ninoslav
AU  - Spremo-Potparević, Biljana
AU  - Zivković, L.
AU  - Milicević, Z.
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/535
AB  - 8-chloro-cyclic adenosine 3,5-monophosphate (8-Cl-cAMP) is the most potent cAMP analog that selectively inhibits a variety of cancer cell lines in vitro and tumors in vivo. Its action toward a variety of tumors, especially when coupled with other antitumor agents, have lead to phase I clinical investigations and recently phase II clinical investigations. Until today, very little was done to evaluate its genotoxic potential. In order to evaluate its genotoxic potential we used the cytogenetic and cytokinesis block micronucleus assay in vitro on peripheral blood lymphocytes of healthy individuals. In three concentrations (1 mu M, 5 mu M and 15 mu M), 8-Cl-cAMP in normal human peripheral blood lymphocytes did not induce any cytogenetic aberrations of the structural type (chromatid breakage, isochromatid breakage and gaps), but did induce premature centromere separation (PCS) at all respective doses and increased the frequency of micronuclei (p < 0.05) only at the highest dose (15 mu M). Antiproliferative action of 8-Cl-cAMP was estimated by using the cytokinesis block nuclear division index (NDI). The results showed a decrease in NDI of cells exposed to all doses of 8-Cl-cAMP when compared to control. Therefore, the overall results show a genotoxic potential of 8-Cl-cAMP in peripheral blood lymphocytes in vitro.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Russian Journal of Genetics
T1  - A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro
VL  - 44
IS  - 5
SP  - 546
EP  - 552
DO  - 10.1134/S1022795408050062
ER  - 

@article{
author = "Bajić, Vladan and Đelić, Ninoslav and Spremo-Potparević, Biljana and Zivković, L. and Milicević, Z.",
year = "2008",
abstract = "8-chloro-cyclic adenosine 3,5-monophosphate (8-Cl-cAMP) is the most potent cAMP analog that selectively inhibits a variety of cancer cell lines in vitro and tumors in vivo. Its action toward a variety of tumors, especially when coupled with other antitumor agents, have lead to phase I clinical investigations and recently phase II clinical investigations. Until today, very little was done to evaluate its genotoxic potential. In order to evaluate its genotoxic potential we used the cytogenetic and cytokinesis block micronucleus assay in vitro on peripheral blood lymphocytes of healthy individuals. In three concentrations (1 mu M, 5 mu M and 15 mu M), 8-Cl-cAMP in normal human peripheral blood lymphocytes did not induce any cytogenetic aberrations of the structural type (chromatid breakage, isochromatid breakage and gaps), but did induce premature centromere separation (PCS) at all respective doses and increased the frequency of micronuclei (p < 0.05) only at the highest dose (15 mu M). Antiproliferative action of 8-Cl-cAMP was estimated by using the cytokinesis block nuclear division index (NDI). The results showed a decrease in NDI of cells exposed to all doses of 8-Cl-cAMP when compared to control. Therefore, the overall results show a genotoxic potential of 8-Cl-cAMP in peripheral blood lymphocytes in vitro.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Russian Journal of Genetics",
title = "A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro",
volume = "44",
number = "5",
pages = "546-552",
doi = "10.1134/S1022795408050062"
}

Bajić, V., Đelić, N., Spremo-Potparević, B., Zivković, L.,& Milicević, Z.. (2008). A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro. in Russian Journal of Genetics
Maik Nauka/Interperiodica/Springer, New York., 44(5), 546-552.
https://doi.org/10.1134/S1022795408050062

Bajić V, Đelić N, Spremo-Potparević B, Zivković L, Milicević Z. A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro. in Russian Journal of Genetics. 2008;44(5):546-552.
doi:10.1134/S1022795408050062 .

Bajić, Vladan, Đelić, Ninoslav, Spremo-Potparević, Biljana, Zivković, L., Milicević, Z., "A study on the genotoxic effects of 8-Cl-cAMP on human lymphocytes in vitro" in Russian Journal of Genetics, 44, no. 5 (2008):546-552,
https://doi.org/10.1134/S1022795408050062 . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Marković, Biljana
AU  - Dačić, S.
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/530
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - In vitro analysis of clastogenic effects of adrenaline on human lymphocytes
VL  - 60
IS  - 3
SP  - 15
EP  - 16
DO  - 10.2298/ABS0803015D
ER  - 

@article{
author = "Đelić, Ninoslav and Spremo-Potparević, Biljana and Živković, Lada and Marković, Biljana and Dačić, S.",
year = "2008",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "In vitro analysis of clastogenic effects of adrenaline on human lymphocytes",
volume = "60",
number = "3",
pages = "15-16",
doi = "10.2298/ABS0803015D"
}

Đelić, N., Spremo-Potparević, B., Živković, L., Marković, B.,& Dačić, S.. (2008). In vitro analysis of clastogenic effects of adrenaline on human lymphocytes. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 60(3), 15-16.
https://doi.org/10.2298/ABS0803015D

Đelić N, Spremo-Potparević B, Živković L, Marković B, Dačić S. In vitro analysis of clastogenic effects of adrenaline on human lymphocytes. in Archives of Biological Sciences. 2008;60(3):15-16.
doi:10.2298/ABS0803015D .

Đelić, Ninoslav, Spremo-Potparević, Biljana, Živković, Lada, Marković, Biljana, Dačić, S., "In vitro analysis of clastogenic effects of adrenaline on human lymphocytes" in Archives of Biological Sciences, 60, no. 3 (2008):15-16,
https://doi.org/10.2298/ABS0803015D . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Nešić, Ivana
AU  - Stanimirović, Zoran
AU  - Jovanović, S.
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/470
AB  - Thyroid hormones enhance aerobic metabolism favoring oxidative stress which may lead to covalent damage of various molecules including DNA. Previous investigations revealed that thyroid hormones induce DNA damage on human lymphocytes and sperm in the in vitro Comet assay. However, cytogenetic evaluation of genotoxic effects of thyroxine gave equivocal results: increase of sister chromatid exchanges, and no incerase of micronuclei in cultured human lymphocytes. Therefore, the aim of the present study was to further evaluate the possible genotoxic effects of thyroxine using in vivo cytogenetic test on Swiss albino mice. Three experimental concentrations of thyroxine were used (0.1 mg/kg, 0.5 mg/kg and 2.5 mg/kg). The mice were divided into several groups depending on the duration of the treatment with thyroxine. Thus, we treated mice for 1, 3, 7 and 10 days. Positive (Nmethyl- N'-nitro-N-nitrosoguanidine) and negative controls were also formed for the same time periods. Cytogenetic endpoinds (numerical and structural aberrations, chormosome gaps and breaks) were analysed in bone marrow cells from femures. The results obtained in this investigation showed that thyroxine has not induced chromosome damage or aberrations. This is in agreement with our previous analysis of micronuclei in human peripheral blood lymophocytes treated with thyroxine. On the other hand, we observed a decrease of mitotic index especially in animals treated for a longer period of time with the highest dose of thyroxine. Therefore, it can be concluded that thyroxine does not induce genotoxic effects which could be detected by cytogenetic analysis.
AB  - Tireoidni hormoni podstiču aerobni metabolizam favorizujući oksidativni stres koji može da dovede do kovalentnih oštećenja različitih molekula uključujući i DNK. U prethodnim istraživanjima otkriveno je da tireoidni hormoni indukuju oštećenja molekula DNK u humanim limfocitima i spermi u in vitro Komet testu. Međutim, citogenetička evaluacija genotoksičnih efekata tiroksina dala je kontradiktorne rezultate: povećanje razmena sestrinskih hromatida bez porasta učesalosti mikronukleusa u kulturama humanih limfocita. Stoga je cilj istraživanja u ovom radu bio da dodatno ispitamo moguće genotoksične efekte tiroksina koristeć i in vitro citogenetički test na Swiss albino miševima. Upotrebljene su tri eksperimentalne koncentracije tiroksina (0,1 mg/kg, 0,5 mg/kg and 2.5 mg/kg). Miševi su podeljeni u nekoliko grupa zavisno od dužine tretmana tiroksinom: 1, 3, 7 i 10 dana. U istim vremenskim periodima miševi su tretirani pozitivnom (N-metil-N'-nitro-N-nitrozogvanidin) i negativnom kontrolom. Analizirani su citogenetički parametri (numeričke i strukturne aberacije hromozoma, gapovi i prekidi na hromozomima) u ćelijama kostne srži izolovanim iz femura. Rezultati dobijeni u ovom istraživanju ukazuju da tiroksin ne indukuje hromozomske prekide i aberacije, što je u saglasnosti sa našim prethodnim zapažanjima na humanim limfocitima u kulturi. Istovremeno, primetili smo smanjenje mitotskog indeksa, naročito kod životinja tretiranih u dužem vremenskom periodu sa visokim dozama tiroksina. Prema tome, može se zaključiti da tiroksin ne indukuje genotoksične efekte koji mogu da se detektuju citogenetičkim analizama.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Evaluation of the genotoxic effects of thyroxine using in vivo cytogenetic test on Swiss albino mice
T1  - Evaluacija genotoksičnih efekata tiroksina primenom in vivo citogenetičkog testa na Swiss albino miševima
VL  - 57
IS  - 5-6
SP  - 487
EP  - 495
DO  - 10.2298/AVB0706487D
ER  - 

@article{
author = "Đelić, Ninoslav and Nešić, Ivana and Stanimirović, Zoran and Jovanović, S.",
year = "2007",
abstract = "Thyroid hormones enhance aerobic metabolism favoring oxidative stress which may lead to covalent damage of various molecules including DNA. Previous investigations revealed that thyroid hormones induce DNA damage on human lymphocytes and sperm in the in vitro Comet assay. However, cytogenetic evaluation of genotoxic effects of thyroxine gave equivocal results: increase of sister chromatid exchanges, and no incerase of micronuclei in cultured human lymphocytes. Therefore, the aim of the present study was to further evaluate the possible genotoxic effects of thyroxine using in vivo cytogenetic test on Swiss albino mice. Three experimental concentrations of thyroxine were used (0.1 mg/kg, 0.5 mg/kg and 2.5 mg/kg). The mice were divided into several groups depending on the duration of the treatment with thyroxine. Thus, we treated mice for 1, 3, 7 and 10 days. Positive (Nmethyl- N'-nitro-N-nitrosoguanidine) and negative controls were also formed for the same time periods. Cytogenetic endpoinds (numerical and structural aberrations, chormosome gaps and breaks) were analysed in bone marrow cells from femures. The results obtained in this investigation showed that thyroxine has not induced chromosome damage or aberrations. This is in agreement with our previous analysis of micronuclei in human peripheral blood lymophocytes treated with thyroxine. On the other hand, we observed a decrease of mitotic index especially in animals treated for a longer period of time with the highest dose of thyroxine. Therefore, it can be concluded that thyroxine does not induce genotoxic effects which could be detected by cytogenetic analysis., Tireoidni hormoni podstiču aerobni metabolizam favorizujući oksidativni stres koji može da dovede do kovalentnih oštećenja različitih molekula uključujući i DNK. U prethodnim istraživanjima otkriveno je da tireoidni hormoni indukuju oštećenja molekula DNK u humanim limfocitima i spermi u in vitro Komet testu. Međutim, citogenetička evaluacija genotoksičnih efekata tiroksina dala je kontradiktorne rezultate: povećanje razmena sestrinskih hromatida bez porasta učesalosti mikronukleusa u kulturama humanih limfocita. Stoga je cilj istraživanja u ovom radu bio da dodatno ispitamo moguće genotoksične efekte tiroksina koristeć i in vitro citogenetički test na Swiss albino miševima. Upotrebljene su tri eksperimentalne koncentracije tiroksina (0,1 mg/kg, 0,5 mg/kg and 2.5 mg/kg). Miševi su podeljeni u nekoliko grupa zavisno od dužine tretmana tiroksinom: 1, 3, 7 i 10 dana. U istim vremenskim periodima miševi su tretirani pozitivnom (N-metil-N'-nitro-N-nitrozogvanidin) i negativnom kontrolom. Analizirani su citogenetički parametri (numeričke i strukturne aberacije hromozoma, gapovi i prekidi na hromozomima) u ćelijama kostne srži izolovanim iz femura. Rezultati dobijeni u ovom istraživanju ukazuju da tiroksin ne indukuje hromozomske prekide i aberacije, što je u saglasnosti sa našim prethodnim zapažanjima na humanim limfocitima u kulturi. Istovremeno, primetili smo smanjenje mitotskog indeksa, naročito kod životinja tretiranih u dužem vremenskom periodu sa visokim dozama tiroksina. Prema tome, može se zaključiti da tiroksin ne indukuje genotoksične efekte koji mogu da se detektuju citogenetičkim analizama.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Evaluation of the genotoxic effects of thyroxine using in vivo cytogenetic test on Swiss albino mice, Evaluacija genotoksičnih efekata tiroksina primenom in vivo citogenetičkog testa na Swiss albino miševima",
volume = "57",
number = "5-6",
pages = "487-495",
doi = "10.2298/AVB0706487D"
}

Đelić, N., Nešić, I., Stanimirović, Z.,& Jovanović, S.. (2007). Evaluation of the genotoxic effects of thyroxine using in vivo cytogenetic test on Swiss albino mice. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(5-6), 487-495.
https://doi.org/10.2298/AVB0706487D

Đelić N, Nešić I, Stanimirović Z, Jovanović S. Evaluation of the genotoxic effects of thyroxine using in vivo cytogenetic test on Swiss albino mice. in Acta Veterinaria-Beograd. 2007;57(5-6):487-495.
doi:10.2298/AVB0706487D .

Đelić, Ninoslav, Nešić, Ivana, Stanimirović, Zoran, Jovanović, S., "Evaluation of the genotoxic effects of thyroxine using in vivo cytogenetic test on Swiss albino mice" in Acta Veterinaria-Beograd, 57, no. 5-6 (2007):487-495,
https://doi.org/10.2298/AVB0706487D . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Đelić, Dijana
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Marković, Biljana
AU  - Lozanče, Olivera
AU  - Blagojević, Miloš
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/455
AB  - Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 mu M to 50 mu M of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species.
PB  - Soc Brasil Genetica, Ribeirao Pret
T2  - Genetics and Molecular Biology
T1  - Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes
VL  - 30
IS  - 4
SP  - 1144
EP  - 1149
DO  - 10.1590/S1415-47572007000600019
ER  - 

@article{
author = "Đelić, Ninoslav and Đelić, Dijana and Spremo-Potparević, Biljana and Živković, Lada and Marković, Biljana and Lozanče, Olivera and Blagojević, Miloš",
year = "2007",
abstract = "Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 mu M to 50 mu M of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species.",
publisher = "Soc Brasil Genetica, Ribeirao Pret",
journal = "Genetics and Molecular Biology",
title = "Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes",
volume = "30",
number = "4",
pages = "1144-1149",
doi = "10.1590/S1415-47572007000600019"
}

Đelić, N., Đelić, D., Spremo-Potparević, B., Živković, L., Marković, B., Lozanče, O.,& Blagojević, M.. (2007). Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes. in Genetics and Molecular Biology
Soc Brasil Genetica, Ribeirao Pret., 30(4), 1144-1149.
https://doi.org/10.1590/S1415-47572007000600019

Đelić N, Đelić D, Spremo-Potparević B, Živković L, Marković B, Lozanče O, Blagojević M. Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes. in Genetics and Molecular Biology. 2007;30(4):1144-1149.
doi:10.1590/S1415-47572007000600019 .

Đelić, Ninoslav, Đelić, Dijana, Spremo-Potparević, Biljana, Živković, Lada, Marković, Biljana, Lozanče, Olivera, Blagojević, Miloš, "Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes" in Genetics and Molecular Biology, 30, no. 4 (2007):1144-1149,
https://doi.org/10.1590/S1415-47572007000600019 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2812
AB  - Premature centromere division (PCD) of the chromosome 18 was analyzed by using fluorescent in situ hybridization (FISH) on interphase peripheral blood lymphocytes isolated from six sporadic Alzheimer disease (AD) patients and six healthy elderly controls. Results of FISH analysis revealed that chromosome 18 expressed PCD in 5.18% interphase nuclei of AD patients, and in 2.59% interphase nuclei of age-matched controls (p < 0.05). Our study also showed that hypoploidy and hyperploidy frequency for chromosome 18 exhibited a statistically significant increase in the AD group compared to the control one. The increase in spontaneous aneuploidy of chromosome 18 in AD patients which is correlated with PCD shows that deregulation of the time of centromere separation can be considered as a manifestation of chromosome instability leading to aneuploidy.
PB  - Elsevier
T2  - Mechanisms of Ageing and Development
T1  - Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes of Alzheimer disease patients
VL  - 127
IS  - 12
SP  - 892
EP  - 896
DO  - 10.1016/j.mad.2006.09.004
ER  - 

@article{
author = "Živković, Lada and Spremo-Potparević, Biljana and Đelić, Ninoslav and Bajić, Vladan",
year = "2006",
abstract = "Premature centromere division (PCD) of the chromosome 18 was analyzed by using fluorescent in situ hybridization (FISH) on interphase peripheral blood lymphocytes isolated from six sporadic Alzheimer disease (AD) patients and six healthy elderly controls. Results of FISH analysis revealed that chromosome 18 expressed PCD in 5.18% interphase nuclei of AD patients, and in 2.59% interphase nuclei of age-matched controls (p < 0.05). Our study also showed that hypoploidy and hyperploidy frequency for chromosome 18 exhibited a statistically significant increase in the AD group compared to the control one. The increase in spontaneous aneuploidy of chromosome 18 in AD patients which is correlated with PCD shows that deregulation of the time of centromere separation can be considered as a manifestation of chromosome instability leading to aneuploidy.",
publisher = "Elsevier",
journal = "Mechanisms of Ageing and Development",
title = "Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes of Alzheimer disease patients",
volume = "127",
number = "12",
pages = "892-896",
doi = "10.1016/j.mad.2006.09.004"
}

Živković, L., Spremo-Potparević, B., Đelić, N.,& Bajić, V.. (2006). Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes of Alzheimer disease patients. in Mechanisms of Ageing and Development
Elsevier., 127(12), 892-896.
https://doi.org/10.1016/j.mad.2006.09.004

Živković L, Spremo-Potparević B, Đelić N, Bajić V. Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes of Alzheimer disease patients. in Mechanisms of Ageing and Development. 2006;127(12):892-896.
doi:10.1016/j.mad.2006.09.004 .

Živković, Lada, Spremo-Potparević, Biljana, Đelić, Ninoslav, Bajić, Vladan, "Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes of Alzheimer disease patients" in Mechanisms of Ageing and Development, 127, no. 12 (2006):892-896,
https://doi.org/10.1016/j.mad.2006.09.004 . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Spremo-Potparević, Biljana
AU  - Bajić, Vladan
AU  - Đelić, D
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/430
AB  - Thyroid hormones enhance the metabolic rate and the aerobic metabolism favoring oxidative stress, which is accompanied by induction of damage to cellular macromolecules including the DNA. The aim of the present study was to investigate the ability of thyroxine to induce sister chromatid exchange and micronuclei, and to modulate cell-cycle kinetics in cultured human lymphocytes. Eight experimental concentrations of thyroxine were used, ranging from 2 x 10(-9) to 0.5 x 10(-4) M. Treatment with thyroxine increased the frequency of SCE per cell at the higher concentrations (1.5 x 10(-6), 0.5 10(-5), 1.5 x 10(-5) and 0.5 x 10(-4) M). On the other hand, there were no significant aneugenic and/or clastogenic effects observed in the cytokinesis-block micronucleus assay. The results show that thyroxine acted as a relatively weak clastogen compared with the positive control N-methyl-N -nitro-N-nitrosoguanidine (MNNG). In addition to the genotoxic effects, two high concentrations of thyroxine decreased the mitotic index and caused cell-cycle delay. In conclusion, thyroxine exhibited weak clastogenic effects only at high concentrations. Therefore, effects in humans might appear in cases of acute thyroxine overdose.
PB  - Elsevier Science Bv, Amsterdam
T2  - Mutation Research-Genetic Toxicology and Environmental Mutagenesis
T1  - Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro
VL  - 604
IS  - 1-2
SP  - 1
EP  - 7
DO  - 10.1016/j.mrgentox.2005.11.013
ER  - 

@article{
author = "Đelić, Ninoslav and Spremo-Potparević, Biljana and Bajić, Vladan and Đelić, D",
year = "2006",
abstract = "Thyroid hormones enhance the metabolic rate and the aerobic metabolism favoring oxidative stress, which is accompanied by induction of damage to cellular macromolecules including the DNA. The aim of the present study was to investigate the ability of thyroxine to induce sister chromatid exchange and micronuclei, and to modulate cell-cycle kinetics in cultured human lymphocytes. Eight experimental concentrations of thyroxine were used, ranging from 2 x 10(-9) to 0.5 x 10(-4) M. Treatment with thyroxine increased the frequency of SCE per cell at the higher concentrations (1.5 x 10(-6), 0.5 10(-5), 1.5 x 10(-5) and 0.5 x 10(-4) M). On the other hand, there were no significant aneugenic and/or clastogenic effects observed in the cytokinesis-block micronucleus assay. The results show that thyroxine acted as a relatively weak clastogen compared with the positive control N-methyl-N -nitro-N-nitrosoguanidine (MNNG). In addition to the genotoxic effects, two high concentrations of thyroxine decreased the mitotic index and caused cell-cycle delay. In conclusion, thyroxine exhibited weak clastogenic effects only at high concentrations. Therefore, effects in humans might appear in cases of acute thyroxine overdose.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Mutation Research-Genetic Toxicology and Environmental Mutagenesis",
title = "Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro",
volume = "604",
number = "1-2",
pages = "1-7",
doi = "10.1016/j.mrgentox.2005.11.013"
}

Đelić, N., Spremo-Potparević, B., Bajić, V.,& Đelić, D.. (2006). Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis
Elsevier Science Bv, Amsterdam., 604(1-2), 1-7.
https://doi.org/10.1016/j.mrgentox.2005.11.013

Đelić N, Spremo-Potparević B, Bajić V, Đelić D. Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis. 2006;604(1-2):1-7.
doi:10.1016/j.mrgentox.2005.11.013 .

Đelić, Ninoslav, Spremo-Potparević, Biljana, Bajić, Vladan, Đelić, D, "Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro" in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 604, no. 1-2 (2006):1-7,
https://doi.org/10.1016/j.mrgentox.2005.11.013 . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Spremo-Potparević, Biljana
AU  - Marković, Biljana
AU  - Živković, Lada
AU  - Đelić, Dijana J.
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/379
AB  - Metabolic conversion of oestrogen phenolic groups may create conditions of oxidative stress accompanied by damage of cellular macromolecules including DNA. The aim of this investigation was to evaluate the cell cycle kinetics and possible cytogenetic changes in cultured human peripheral blood lymphocytes exposed to seven experimental concentrations of 17β-oestradiol (range 10-10 M to 10-4 M). Cell cycle kinetics was analyzed on metaphase spreads prepared for a standard analysis of sister-chromatid exchanges (SCEs) stained by fluorescent-plus-Giemsa (FPG) technique. Cytogenetic changes were monitored by analysis of chromosome damage (gaps and breaks), structural and numerical aberrations. On the basis of the obtained results it can be concluded that oestradiol has no significant influence on cell cycle kinetics and mitotic index of cultured human lymphocytes. However, at estradiol concentration of 7×10-6 M, and at higher concentrations used in this experiment, there was a significant increase of gaps, breaks and aneuploidies. On the other hand, oestradiol treatment has not changed the frequency of polyploid cells. Therefore, it can be concluded that high concentrations of oestradiol pose some genetic risk detectable at cytogenetic level.
AB  - Metabolička konverzija fenolnih grupa estrogenih hormona može da dovede do oksidativnog stresa praćenog oštećenjima različitih makromolekula u eliji, uključujući DNK. Cilj ovog istraživanja je evaluacija kinetike proliferacije i mogućih citogenetičkih promena u kulturama humanih limfocita pod dejstvom sedam eksperimentalnih koncentracija 17β-estradiola (opseg od 10-10M do10-4 M). Kinetika proliferacije limfocita analizirana je na metafaznim figurama obojenim tehnikom FPG za standardne analize razmena sestrinskih hromatida (SCE). Citogenetičke promene praćene su analizama hromozomskih oštećenja (gapovi i prekidi), strukturnih i numeričkih aberacija hromozoma. Na osnovu dobijenih rezultata može se zaključiti da estradiol ne utiče značajno na mitotsku aktivnost i kinetiku proliferacije limfocita u kulturi. Međutim, pri koncentraciji od 7×10-6 M, kao i pri višim eksperimentalnim koncentracijama korišćenim u ovim eksperimentima, zapažen je porast gapova, prekida i aneuploidija. S druge strane, tretman estradiolom ne menja učestalost poliploidnih ćelija. Prema tome, može se zaključiti da visoke koncentracije estradiola izazivaju izvestan genetički rizik koji se može detektovati na citogenetičkom nivou.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro
T1  - Kinetika proliferacije i citogenetičke promene u humanim limfocitima pod dejstvom estradiola in vitro
VL  - 56
IS  - 1
SP  - 37
EP  - 48
DO  - 10.2298/AVB0601037D
ER  - 

@article{
author = "Đelić, Ninoslav and Spremo-Potparević, Biljana and Marković, Biljana and Živković, Lada and Đelić, Dijana J.",
year = "2006",
abstract = "Metabolic conversion of oestrogen phenolic groups may create conditions of oxidative stress accompanied by damage of cellular macromolecules including DNA. The aim of this investigation was to evaluate the cell cycle kinetics and possible cytogenetic changes in cultured human peripheral blood lymphocytes exposed to seven experimental concentrations of 17β-oestradiol (range 10-10 M to 10-4 M). Cell cycle kinetics was analyzed on metaphase spreads prepared for a standard analysis of sister-chromatid exchanges (SCEs) stained by fluorescent-plus-Giemsa (FPG) technique. Cytogenetic changes were monitored by analysis of chromosome damage (gaps and breaks), structural and numerical aberrations. On the basis of the obtained results it can be concluded that oestradiol has no significant influence on cell cycle kinetics and mitotic index of cultured human lymphocytes. However, at estradiol concentration of 7×10-6 M, and at higher concentrations used in this experiment, there was a significant increase of gaps, breaks and aneuploidies. On the other hand, oestradiol treatment has not changed the frequency of polyploid cells. Therefore, it can be concluded that high concentrations of oestradiol pose some genetic risk detectable at cytogenetic level., Metabolička konverzija fenolnih grupa estrogenih hormona može da dovede do oksidativnog stresa praćenog oštećenjima različitih makromolekula u eliji, uključujući DNK. Cilj ovog istraživanja je evaluacija kinetike proliferacije i mogućih citogenetičkih promena u kulturama humanih limfocita pod dejstvom sedam eksperimentalnih koncentracija 17β-estradiola (opseg od 10-10M do10-4 M). Kinetika proliferacije limfocita analizirana je na metafaznim figurama obojenim tehnikom FPG za standardne analize razmena sestrinskih hromatida (SCE). Citogenetičke promene praćene su analizama hromozomskih oštećenja (gapovi i prekidi), strukturnih i numeričkih aberacija hromozoma. Na osnovu dobijenih rezultata može se zaključiti da estradiol ne utiče značajno na mitotsku aktivnost i kinetiku proliferacije limfocita u kulturi. Međutim, pri koncentraciji od 7×10-6 M, kao i pri višim eksperimentalnim koncentracijama korišćenim u ovim eksperimentima, zapažen je porast gapova, prekida i aneuploidija. S druge strane, tretman estradiolom ne menja učestalost poliploidnih ćelija. Prema tome, može se zaključiti da visoke koncentracije estradiola izazivaju izvestan genetički rizik koji se može detektovati na citogenetičkom nivou.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro, Kinetika proliferacije i citogenetičke promene u humanim limfocitima pod dejstvom estradiola in vitro",
volume = "56",
number = "1",
pages = "37-48",
doi = "10.2298/AVB0601037D"
}

Đelić, N., Spremo-Potparević, B., Marković, B., Živković, L.,& Đelić, D. J.. (2006). Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(1), 37-48.
https://doi.org/10.2298/AVB0601037D

Đelić N, Spremo-Potparević B, Marković B, Živković L, Đelić DJ. Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro. in Acta Veterinaria-Beograd. 2006;56(1):37-48.
doi:10.2298/AVB0601037D .

Đelić, Ninoslav, Spremo-Potparević, Biljana, Marković, Biljana, Živković, Lada, Đelić, Dijana J., "Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro" in Acta Veterinaria-Beograd, 56, no. 1 (2006):37-48,
https://doi.org/10.2298/AVB0601037D . .