@article{
author = "Marinković, Darko and Aleksić-Kovačević, Sanja and Krstić, Vanja and Knežević, Milijana",
year = "2009",
abstract = "Cranial mesenteric arteries of 18 sacrificed necropsied horses of both sexes and different age groups were described in this paper. After macroscopic examination tissue samples for pathohistological examinations were routinely processed and stained with hematoxylin eosine (HE), Weigert van Gieson and Periodic-Acid-Schiff (PAS) staining. Immunohistochemical staining was performed on selected sections using avidin-biotin-peroxidase complex technique for ásmooth muscle actin (α-SMA). Enlarged, thickened cranial mesenteric arteries with a hardelastic consistency and narrowed lumen, were macroscopically evident in all examined sick horses. Live larvae of Strongylus vulgaris, situated free in the lumen, or attached to the intima of the blood vessel or incorporated in the thrombus were noted macroscopically in 37.5% examined sick horses. Inflammatory and fibrous changes were noticed and were present in the intima, media and adventitia of the blood vessel. The inflammatory infiltrate in the intima of the cranial mesenteric artery consisted of eosinophils, macrophages, plasma cells, lymphocytes and rare multinucleated giant cells and was situated mostly close to the Strongylus vulgaris larvae. Beside inflammatory changes, intimal fibrosis and extension, characterized by increased proliferation of α-SMA positive cells, was notable. Lesions of the internal elastic lamina consequently led to an inflammatory and fibrotic reaction in the tunica media. Fibrosis of the media characterized by the presence of connective tissue cells and fibers, as well as smoothmuscle cells, was present in 93.75% examined sick horses. Inflammation and fibrosis were mildest in the adventitia. Lesions of vasa vasorum were present in 81.25% examined sick animals were characterized by fibrosis, obliteration, perivascular cellular infiltration, mostly with eosinophils and findings of intimal bodies. All described changes are characteristic for cranial mesenteric artery verminous arteritis., U ovom radu su ispitane kranijalne mezeneterijalne arterije 18 obdukovanih žrtvovanih konja različitog pola i starosti. Arterije su makroskopski pregledane i uzimani su tkivni isečci za patohistološka ispitivanja. Materijal za histološka ispitivanja fiksiran je u 10% puferizovanom formalinu i posle uobičajene procedure ukalupljen u parafinske blokove. Patohistološki preparati bojeni su hematoksilinom i eozinom (HE), Vajgert van Gison metodom i PAS metodom. Odabrani isečci imunohistohemijski su bojeni avidin-biotin-peroksidaza tehnikom za α-smooth muscle actin (α-SMA). Makroskopski, kranijalne mezenterijalne arterije svih ispitanih konja bile su uvećane, zadebljale, čvrsto-elastične konzistencije i suženog lumena. Žive larve Strongylus vulgaris slobodne u lumenu ili pričvršćene za intimu krvnog suda ili inkorporisane u trombnu masu uočene su makroskopski kod 37,5% obolelih konja. Mikroskopski su uočene promene inflamatornog do fibroznog karaktera i bile su zastupljene u intimi, mediji i adventiciji krvnog suda. Inflamatorni infiltrat u intimi su činili eozinofilni granulociti, makrofage, plazma ćelije, limfociti i višejedarne džinovske ćelije i ovaj infiltrat bio je prisutan uglavnom u blizini larve Strongylus vulgaris. Pored inflamatornih promena uočeno je zadebljavanje sa fibrozom intime koja se karakterisala proliferacijom vezivno-tkivnih ćelija i vlakana, kao i glatko-mišićnih ćelija. Lezije na unutrašnjoj elastičnoj lamini posledično su dovodile do inflamacije i fibroze tunike medije. Fibroza medije se karakterisala prisustvom vezivno-tkivnih ćelija i vlakana, kao i glatko-mišićnih ćelija i bila je prisutna kod 93,75% obolelih konja. Inflamacija i fibroza su najslabije bile izražene u adventiciji, a lezije vasa vasorum su bile prisutne kod 81,25% pregledanih obolelih životinja i karakterisale su se fibrozom, obliteracijom, perivaskularnom ćelijskom infiltracijom, uglavnom sa eozinofilima, kao i nalazom intimalnih tela. Sve opisane promene su karakteristične za verminozni arteritis kranijalne mezenterijalne arterije.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Morphological findings in the cranial mesenteric artery of horses with verminous arteritis, Morfološki nalaz na kranijalnim mezenterijalnim arterijama konja sa verminoznim arteritisom",
volume = "59",
number = "2-3",
pages = "231-241",
doi = "10.2298/AVB0903231M"
}
