TY  - JOUR
AU  - Milošević, Ivan
AU  - Radovanović, Anita
AU  - Danilović-Luković, Jelena
AU  - Lužajić Božinovski, Tijana
AU  - Sourice-Petit, Sophie
AU  - Beck-Cormier, Sarah
AU  - Guicheux, Jerome
AU  - Vejnović, Branislav
AU  - Kovačević-Filipović, Milica
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1588
AB  - Maternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, influences the process of offspring endochondral ossification affecting proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups. Seven-day-old male pups (n=18) derived from control dams and dams treated with a low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water during pregnancy and lactation were used. Histomorphometric analysis of pups tibia proximal growth plate, expression of mRNA, immunohistochemical and histochemical visualization of extracellular matrix components was performed. The length of the tibia was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical and overt form were lower while the amount of glycosaminoglycans was higher when compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA indicated altered osteoclasts function while lower expression of dentin matrix acid protein-1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised bone formation in the overt form of hypothyroidism. The subclinical form of maternal hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in growth plate formation.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation
VL  - 68
IS  - 3
SP  - 301
EP  - 320
DO  - 10.2478/acve-2018-0026
ER  - 

@article{
author = "Milošević, Ivan and Radovanović, Anita and Danilović-Luković, Jelena and Lužajić Božinovski, Tijana and Sourice-Petit, Sophie and Beck-Cormier, Sarah and Guicheux, Jerome and Vejnović, Branislav and Kovačević-Filipović, Milica",
year = "2018",
abstract = "Maternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, influences the process of offspring endochondral ossification affecting proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups. Seven-day-old male pups (n=18) derived from control dams and dams treated with a low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water during pregnancy and lactation were used. Histomorphometric analysis of pups tibia proximal growth plate, expression of mRNA, immunohistochemical and histochemical visualization of extracellular matrix components was performed. The length of the tibia was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical and overt form were lower while the amount of glycosaminoglycans was higher when compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA indicated altered osteoclasts function while lower expression of dentin matrix acid protein-1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised bone formation in the overt form of hypothyroidism. The subclinical form of maternal hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in growth plate formation.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation",
volume = "68",
number = "3",
pages = "301-320",
doi = "10.2478/acve-2018-0026"
}

Milošević, I., Radovanović, A., Danilović-Luković, J., Lužajić Božinovski, T., Sourice-Petit, S., Beck-Cormier, S., Guicheux, J., Vejnović, B.,& Kovačević-Filipović, M.. (2018). Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(3), 301-320.
https://doi.org/10.2478/acve-2018-0026

Milošević I, Radovanović A, Danilović-Luković J, Lužajić Božinovski T, Sourice-Petit S, Beck-Cormier S, Guicheux J, Vejnović B, Kovačević-Filipović M. Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation. in Acta Veterinaria-Beograd. 2018;68(3):301-320.
doi:10.2478/acve-2018-0026 .

Milošević, Ivan, Radovanović, Anita, Danilović-Luković, Jelena, Lužajić Božinovski, Tijana, Sourice-Petit, Sophie, Beck-Cormier, Sarah, Guicheux, Jerome, Vejnović, Branislav, Kovačević-Filipović, Milica, "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation" in Acta Veterinaria-Beograd, 68, no. 3 (2018):301-320,
https://doi.org/10.2478/acve-2018-0026 . .