TY  - JOUR
AU  - Nišavić, Jakov
AU  - Milić, Nenad
AU  - Radalj, Andrea
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1942
AB  - Background. Coronaviruses (CoVs) have been recognized in veterinary virology for a long time and comprise a large group of RNA viruses responsible for enteric, respiratory, hepatic, and neurologic diseases in a variety of animal species and humans. These viruses are very adaptable considering their highly error-prone replication process and recombination ability, resulting in remarkable mutability and efficient expansion of their host range and tissue tropism. Scope and Approach. In the recent past, after the outbreaks caused by SARS-CoV in 2002 and MERS-CoV in 2012, CoVs became a research focus in the scientific community. Moreover, the ongoing SARS-CoV-2 pandemic raised more questions concerning the threats posed by these viruses. Several significant examples of coronaviruses jumping the species barrier and changing their tropism have been reported in the past, and novel viruses of both animals and humans have appeared as a consequence. This paper reviews some of the examples of CoV mutability and the most notable animal coronaviruses of veterinary relevance. Key Findings and Conclusions. There is still no proof that the novel virus SARS-CoV-2 can be transmitted to humans from domestic animals, and its recent cross-species jump is currently being intensively researched. Intensified and diverse human activities that lead to the disruption of ecosystems contribute to the increased risk of contact with animals that might represent virus reservoirs. The need for constant surveillance of CoVs and expanded studies of their virological traits, mutation mechanisms, diversity, prophylactic and therapeutic measures highlight the key role of both veterinarians and medical doctors in order to preserve the health of the human population.
AB  - Uvod. Koronavirusi se u okviru veterinarske virusologije izučavaju već dugi niz godina i predstavljaju veliku grupaciju RNK virusa odgovornih za gastrointestinalna, respiratorna i neurološka oboljenja velikog broja životinja i ljudi. Navedeni virusi su visoko adaptibilni s obzirom na njihov jedinstven mehanizam replikacije i sposobnost mutiranja, pri čemu dolazi do česte transmisije koronavirusa među različitim vrstama, kao i promena u njihovom tropizmu. Cilj i pristup. U skorijoj prošlosti su zabeležene epidemije izazvane virusima SARS-CoV 2002., odnosno MERS-CoV 2012. godine, nakon kojih su koronavirusi postali tema mnogih ispitivanja. Nove nepoznanice i pitanja vezana za pretnju koju koronavirusi predstavljaju izazvala je trenutna pandemija izazvana virusom SARS-CoV-2. Postoji veliki broj značajnih primera koronavirusa koji su preskočili barijeru vrste i posledično doveli do pojave novih oboljenja kako životinja, tako i ljudi. U ovom radu su opisani neki od najznačajnih primera koji oslikavaju sposobnost mutacije koronavirusa, kao i osnovne karakteristike koronavirusnih infekcija koje su od velike važnosti za veterinarsku medicinu. Ključni nalazi i zaključak. Do danas ne postoje dokazi da se novootkriveni virus SARSCoV-2 prenosi sa domaćih životinja na ljude, a mehanizam kojim je došlo do prelaska navedenog virusa na ljude se intenzivno ispituje. Sve učestalije aktivnosti čoveka koje dovode do narušavanja ekosistema doprinose povećanju rizika od kontakta sa divljim životinjama koje mogu predstavljati rezervoare virusa. Potreba za kontinuiranim nadzorom nad pojavom infekcija izazvanih koronavirusima, podrobnijim ispitivanjima u poljima virusologije i vakcinologije, kao i za pronalaskom efikasne terapije ovih oboljenja predstavljaju ključnu i zajedničku ulogu veterinara i lekara u cilju očuvanja zdravlja ljudi.
PB  - Fakultet veterinarske medicine, Beograd, Srbija
T2  - Veterinarski Glasnik
T1  - Overview of the most significant coronavirus infections in veterinary medicine
T1  - Pregled najznačajnijih infekcija koronavirusima iz veterinarske perspektive
VL  - 74
IS  - 1
SP  - 1
EP  - 17
DO  - 10.2298/VETGL2001001N
ER  - 

@article{
author = "Nišavić, Jakov and Milić, Nenad and Radalj, Andrea",
year = "2020",
abstract = "Background. Coronaviruses (CoVs) have been recognized in veterinary virology for a long time and comprise a large group of RNA viruses responsible for enteric, respiratory, hepatic, and neurologic diseases in a variety of animal species and humans. These viruses are very adaptable considering their highly error-prone replication process and recombination ability, resulting in remarkable mutability and efficient expansion of their host range and tissue tropism. Scope and Approach. In the recent past, after the outbreaks caused by SARS-CoV in 2002 and MERS-CoV in 2012, CoVs became a research focus in the scientific community. Moreover, the ongoing SARS-CoV-2 pandemic raised more questions concerning the threats posed by these viruses. Several significant examples of coronaviruses jumping the species barrier and changing their tropism have been reported in the past, and novel viruses of both animals and humans have appeared as a consequence. This paper reviews some of the examples of CoV mutability and the most notable animal coronaviruses of veterinary relevance. Key Findings and Conclusions. There is still no proof that the novel virus SARS-CoV-2 can be transmitted to humans from domestic animals, and its recent cross-species jump is currently being intensively researched. Intensified and diverse human activities that lead to the disruption of ecosystems contribute to the increased risk of contact with animals that might represent virus reservoirs. The need for constant surveillance of CoVs and expanded studies of their virological traits, mutation mechanisms, diversity, prophylactic and therapeutic measures highlight the key role of both veterinarians and medical doctors in order to preserve the health of the human population., Uvod. Koronavirusi se u okviru veterinarske virusologije izučavaju već dugi niz godina i predstavljaju veliku grupaciju RNK virusa odgovornih za gastrointestinalna, respiratorna i neurološka oboljenja velikog broja životinja i ljudi. Navedeni virusi su visoko adaptibilni s obzirom na njihov jedinstven mehanizam replikacije i sposobnost mutiranja, pri čemu dolazi do česte transmisije koronavirusa među različitim vrstama, kao i promena u njihovom tropizmu. Cilj i pristup. U skorijoj prošlosti su zabeležene epidemije izazvane virusima SARS-CoV 2002., odnosno MERS-CoV 2012. godine, nakon kojih su koronavirusi postali tema mnogih ispitivanja. Nove nepoznanice i pitanja vezana za pretnju koju koronavirusi predstavljaju izazvala je trenutna pandemija izazvana virusom SARS-CoV-2. Postoji veliki broj značajnih primera koronavirusa koji su preskočili barijeru vrste i posledično doveli do pojave novih oboljenja kako životinja, tako i ljudi. U ovom radu su opisani neki od najznačajnih primera koji oslikavaju sposobnost mutacije koronavirusa, kao i osnovne karakteristike koronavirusnih infekcija koje su od velike važnosti za veterinarsku medicinu. Ključni nalazi i zaključak. Do danas ne postoje dokazi da se novootkriveni virus SARSCoV-2 prenosi sa domaćih životinja na ljude, a mehanizam kojim je došlo do prelaska navedenog virusa na ljude se intenzivno ispituje. Sve učestalije aktivnosti čoveka koje dovode do narušavanja ekosistema doprinose povećanju rizika od kontakta sa divljim životinjama koje mogu predstavljati rezervoare virusa. Potreba za kontinuiranim nadzorom nad pojavom infekcija izazvanih koronavirusima, podrobnijim ispitivanjima u poljima virusologije i vakcinologije, kao i za pronalaskom efikasne terapije ovih oboljenja predstavljaju ključnu i zajedničku ulogu veterinara i lekara u cilju očuvanja zdravlja ljudi.",
publisher = "Fakultet veterinarske medicine, Beograd, Srbija",
journal = "Veterinarski Glasnik",
title = "Overview of the most significant coronavirus infections in veterinary medicine, Pregled najznačajnijih infekcija koronavirusima iz veterinarske perspektive",
volume = "74",
number = "1",
pages = "1-17",
doi = "10.2298/VETGL2001001N"
}

Nišavić, J., Milić, N.,& Radalj, A.. (2020). Overview of the most significant coronavirus infections in veterinary medicine. in Veterinarski Glasnik
Fakultet veterinarske medicine, Beograd, Srbija., 74(1), 1-17.
https://doi.org/10.2298/VETGL2001001N

Nišavić J, Milić N, Radalj A. Overview of the most significant coronavirus infections in veterinary medicine. in Veterinarski Glasnik. 2020;74(1):1-17.
doi:10.2298/VETGL2001001N .

Nišavić, Jakov, Milić, Nenad, Radalj, Andrea, "Overview of the most significant coronavirus infections in veterinary medicine" in Veterinarski Glasnik, 74, no. 1 (2020):1-17,
https://doi.org/10.2298/VETGL2001001N . .

TY  - JOUR
AU  - Milićević, Dragan
AU  - Udovički, Božidar
AU  - Petrović, Zoran
AU  - Janković, Saša
AU  - Radulović, Stamen
AU  - Gurinović, Mirjana
AU  - Rajković, Andreja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2068
AB  - Mycotoxins are chemical hazards of microbiological origin, produced mainly by filamentous fungi during their secondary metabolism. The role of mycotoxins has been recognized in the aetiology of a number of diseases, particularly cancers that belong to non-communicable diseases (NCDs). The NCDs have a leading and growing contribution to preventable deaths and disability across the globe. The NCDs are known as chronic diseases, tend to be of long duration and are the result of a combination of genetic, physiological, environmental and behavioural factors. Following the increased interest in health effects caused by synergisms between natural and synthetic contaminants along the food chain, mycotoxin contamination will continue to be an area of concern for producers, manufacturers, regulatory agencies, researchers and consumers in the future. Considering that their presence in food depends strongly on climatic conditions, in Serbia, recent drought and then flooding confirmed that mycotoxins are one of the foodborne hazards most susceptible to climate change. In this article, we review key aspects of mycotoxin contamination of the food supply chain and attempt to highlight the latest trends and projections for mycotoxin reduction from a Serbian perspective.
AB  - Mikotoksini predstavljaju hemijski hazard mikorbiološkog porekla, proizvod sekundarnog metabolizma pretežno filamentoznih plesni. Značaj mikotoksina najčešće se vezuje za pojavu brojnih oboljenja kod ljudi i životinja, koja pripadaju grupi nezaraznih bolesti (eng. non-communicable diseases). Nezarazne bolesti, (npr. maligni tumori), vodeći su uzroci obolevanja, invalidnosti i prevremenog umiranja (pre 65. godine života) u svetu, a i u našoj sredini (eng. Disability Adjusted Life Years-DALY). Maligna oboljenja se karakterišu dužim vremenom trajanja i nastaju kao posledica interakcije mnogobrojnih faktora kao što su genetski, fiziološki status organizma, prirodno okruženje i biološkog odgovora čovekovog organizma. Sve veće interesovanje za sinergistički efekat sintetskih i prirodnih kontaminenata na zdravlje ljudi, ukazuje na to da kontaminacija mikotoksinima predstavlja idalje oblast od prioritetnog značaja za sve učesnike u lancu hrane. Uzimajući u obzir da kontaminacija hrane mikotoksinima prvenstveno zavisi od klimatskih faktora, ekstremne klimatske pojave kao što su suša i poplave poslednjih godina zabeležene u Srbiji, potvrđuju činjenicu da su mikotoksini jedan od hazarda u lancu hrane na koji klimatske promene imaju najveći uticaj. U ovom radu pokušali smo da analiziramo ključne faktore od značaja za kontaminaciju mikotoksinima, kao i da se ukaže na najnovije trendove i strategije u prevenciji štetnih efekata mikotoksina u lancu hrane, sagledavajući stanje i mogućnosti u Srbiji.
PB  - Beograd : Institut za higijenu i tehnologiju mesa
T2  - Meat Technology
T2  - Meat Technology
T1  - Current status of mycotoxin contamination of food and feeds and associated public health risk in Serbia
T1  - Aktuelna situacija kontaminacije hrane i hrane za životinje mikotoksinima sa osvrtom na javnozdravstveni rizik u Srbiji
VL  - 61
IS  - 1
SP  - 1
EP  - 36
DO  - 10.18485/meattech.2020.61.1.1
ER  - 

@article{
author = "Milićević, Dragan and Udovički, Božidar and Petrović, Zoran and Janković, Saša and Radulović, Stamen and Gurinović, Mirjana and Rajković, Andreja",
year = "2020",
abstract = "Mycotoxins are chemical hazards of microbiological origin, produced mainly by filamentous fungi during their secondary metabolism. The role of mycotoxins has been recognized in the aetiology of a number of diseases, particularly cancers that belong to non-communicable diseases (NCDs). The NCDs have a leading and growing contribution to preventable deaths and disability across the globe. The NCDs are known as chronic diseases, tend to be of long duration and are the result of a combination of genetic, physiological, environmental and behavioural factors. Following the increased interest in health effects caused by synergisms between natural and synthetic contaminants along the food chain, mycotoxin contamination will continue to be an area of concern for producers, manufacturers, regulatory agencies, researchers and consumers in the future. Considering that their presence in food depends strongly on climatic conditions, in Serbia, recent drought and then flooding confirmed that mycotoxins are one of the foodborne hazards most susceptible to climate change. In this article, we review key aspects of mycotoxin contamination of the food supply chain and attempt to highlight the latest trends and projections for mycotoxin reduction from a Serbian perspective., Mikotoksini predstavljaju hemijski hazard mikorbiološkog porekla, proizvod sekundarnog metabolizma pretežno filamentoznih plesni. Značaj mikotoksina najčešće se vezuje za pojavu brojnih oboljenja kod ljudi i životinja, koja pripadaju grupi nezaraznih bolesti (eng. non-communicable diseases). Nezarazne bolesti, (npr. maligni tumori), vodeći su uzroci obolevanja, invalidnosti i prevremenog umiranja (pre 65. godine života) u svetu, a i u našoj sredini (eng. Disability Adjusted Life Years-DALY). Maligna oboljenja se karakterišu dužim vremenom trajanja i nastaju kao posledica interakcije mnogobrojnih faktora kao što su genetski, fiziološki status organizma, prirodno okruženje i biološkog odgovora čovekovog organizma. Sve veće interesovanje za sinergistički efekat sintetskih i prirodnih kontaminenata na zdravlje ljudi, ukazuje na to da kontaminacija mikotoksinima predstavlja idalje oblast od prioritetnog značaja za sve učesnike u lancu hrane. Uzimajući u obzir da kontaminacija hrane mikotoksinima prvenstveno zavisi od klimatskih faktora, ekstremne klimatske pojave kao što su suša i poplave poslednjih godina zabeležene u Srbiji, potvrđuju činjenicu da su mikotoksini jedan od hazarda u lancu hrane na koji klimatske promene imaju najveći uticaj. U ovom radu pokušali smo da analiziramo ključne faktore od značaja za kontaminaciju mikotoksinima, kao i da se ukaže na najnovije trendove i strategije u prevenciji štetnih efekata mikotoksina u lancu hrane, sagledavajući stanje i mogućnosti u Srbiji.",
publisher = "Beograd : Institut za higijenu i tehnologiju mesa",
journal = "Meat Technology, Meat Technology",
title = "Current status of mycotoxin contamination of food and feeds and associated public health risk in Serbia, Aktuelna situacija kontaminacije hrane i hrane za životinje mikotoksinima sa osvrtom na javnozdravstveni rizik u Srbiji",
volume = "61",
number = "1",
pages = "1-36",
doi = "10.18485/meattech.2020.61.1.1"
}

Milićević, D., Udovički, B., Petrović, Z., Janković, S., Radulović, S., Gurinović, M.,& Rajković, A.. (2020). Current status of mycotoxin contamination of food and feeds and associated public health risk in Serbia. in Meat Technology
Beograd : Institut za higijenu i tehnologiju mesa., 61(1), 1-36.
https://doi.org/10.18485/meattech.2020.61.1.1

Milićević D, Udovički B, Petrović Z, Janković S, Radulović S, Gurinović M, Rajković A. Current status of mycotoxin contamination of food and feeds and associated public health risk in Serbia. in Meat Technology. 2020;61(1):1-36.
doi:10.18485/meattech.2020.61.1.1 .

Milićević, Dragan, Udovički, Božidar, Petrović, Zoran, Janković, Saša, Radulović, Stamen, Gurinović, Mirjana, Rajković, Andreja, "Current status of mycotoxin contamination of food and feeds and associated public health risk in Serbia" in Meat Technology, 61, no. 1 (2020):1-36,
https://doi.org/10.18485/meattech.2020.61.1.1 . .

TY  - JOUR
AU  - Milić, Nenad
AU  - Radalj, Andrea
AU  - Nišavić, Jakov
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1621
AB  - Equine herpesvirus type 1(EHV-1) is responsible for respiratory disease in young animals, abortion in pregnant mares and neurological disease, whilst equine herpesvirus 4 (EHV-4) is mainly the causative agent of respiratory disorders and rarely causes abortion. These viruses are considered as one of the most clinically and economically important pathogens of horses and can be detected in a range of tissues. Serological methods are used to detect the presence and titre of specific antibodies to equine herpesvirus 1 and 4 in the sera of examined horses and are useful in epizootiological studies. Commercially available ELISA kits are able to differentiate specific EHV-1 and EHV-4 antibodies. Equine herpesvirus-1 and equine herpesvirus-4 can both be isolated using susceptible cells such as primary horse cell cultures and other non-equine cells with visible cytopathic effect. Since standard diagnostic methods can be time consuming and arduous, the scope of many studies has been to develop and confirm the sensitivity and specificity of molecular diagnostic methods. Polymerase chain reaction (PCR) has proved to be a good screening method for the presence of latent infections of horses caused by these viruses, also making possible the rapid identification and differentiation of EHV-1 and-EHV-4 in the examined samples. Real-time PCR is a sensitive, specific and quantitative method that enables the determination of viral kinetics in infected horses. Genome sequencing can be used to discover mutations in the genomes of EHV-1 and EHV-4 as well as to track the spread of their different strains globally. .
AB  - Uvod. Konjski herpesvirus 1 (EHV-1) izaziva respiratorne infekcije mladih konja, promene u CNS-u i pojavu abortusa kod gravidnih kobila. Infekcije izazvane konjskim herpesvirusom 4 (EHV-4) se manifestuju ispoljavanjem respiratornih simptoma oboljenja kod životinja, a ređe pojavom abortusa. Infekcije izazvane prethodno navedenim virusima prisutne su u populaciji konja širom sveta. Cilj i pristup. U cilju laboratorijske dijagnostike, odnosno izrade odgovarajućih epizootioloških studija o prisustvu virusa EHV-1 i EHV-4 u populaciji konja na određenom prostoru, primenjuju se serološke metode kao što su ELISA ili test virus neutralizacije. Primenom navedenih metoda se vrši otkrivanje prisustva i titra specifčnih antitela protiv prethodno navedenih virusa u uzorcima krvnih seruma konja. Ovde treba napomenuti da su danas dostupni i komercijalni kitovi za izvođenje metode ELISA koji omogućavaju diferencijaciju specifčnih EHV-1 i EHV-4 antitela. Konjski herpesvirus 1 i konjski herpesvirus 4 se mogu izolovati na kulturama ćelija poreklom od konja, kao i na drugim ćelijskim linijama, a daju karakterističan citopatogeni efekat. Pored navedenih klasičnih virusoloških metoda, danas su u upotrebi i molekularne metode dijagnostike kao što su PCR ili real-time PCR. Ključni nalazi i zaključak. Lančana reakcija polimeraze (PCR) predstavlja pogodnu screening metodu za brzo i pouzdano otkrivanje prisustva virusa EHV-1 i EHV-4 u ispitivanim uzorcima poreklom od konja. Real-time PCR je osetljiva, specifčna i kvantitativna metoda čijom primenom se takođe može utvrditi prisustvo navedenih virusa u organizmu konja. Metodom sekvenciranja genoma virusa EHV-1 i EHV-4 se mogu utvrditi sličnosti ili razlike između pojedinih sojeva virusa, odnosno može se izvršiti molekularna karakterizacija sojeva virusa EHV-1 i EHV-4 dominantno prisutnih u populaciji konja na određenoj teritoriji. .
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Standard and molecular methods in the diagnostics of infections caused by equine herpesviruses 1 and 4
T1  - Standardne i molekularne metode dijagnostike infekcija izazvanih konjskim herpesvirusima 1 i 4
VL  - 72
IS  - 2
SP  - 68
EP  - 79
DO  - 10.2298/VETGL170227002M
ER  - 

@article{
author = "Milić, Nenad and Radalj, Andrea and Nišavić, Jakov",
year = "2018",
abstract = "Equine herpesvirus type 1(EHV-1) is responsible for respiratory disease in young animals, abortion in pregnant mares and neurological disease, whilst equine herpesvirus 4 (EHV-4) is mainly the causative agent of respiratory disorders and rarely causes abortion. These viruses are considered as one of the most clinically and economically important pathogens of horses and can be detected in a range of tissues. Serological methods are used to detect the presence and titre of specific antibodies to equine herpesvirus 1 and 4 in the sera of examined horses and are useful in epizootiological studies. Commercially available ELISA kits are able to differentiate specific EHV-1 and EHV-4 antibodies. Equine herpesvirus-1 and equine herpesvirus-4 can both be isolated using susceptible cells such as primary horse cell cultures and other non-equine cells with visible cytopathic effect. Since standard diagnostic methods can be time consuming and arduous, the scope of many studies has been to develop and confirm the sensitivity and specificity of molecular diagnostic methods. Polymerase chain reaction (PCR) has proved to be a good screening method for the presence of latent infections of horses caused by these viruses, also making possible the rapid identification and differentiation of EHV-1 and-EHV-4 in the examined samples. Real-time PCR is a sensitive, specific and quantitative method that enables the determination of viral kinetics in infected horses. Genome sequencing can be used to discover mutations in the genomes of EHV-1 and EHV-4 as well as to track the spread of their different strains globally. ., Uvod. Konjski herpesvirus 1 (EHV-1) izaziva respiratorne infekcije mladih konja, promene u CNS-u i pojavu abortusa kod gravidnih kobila. Infekcije izazvane konjskim herpesvirusom 4 (EHV-4) se manifestuju ispoljavanjem respiratornih simptoma oboljenja kod životinja, a ređe pojavom abortusa. Infekcije izazvane prethodno navedenim virusima prisutne su u populaciji konja širom sveta. Cilj i pristup. U cilju laboratorijske dijagnostike, odnosno izrade odgovarajućih epizootioloških studija o prisustvu virusa EHV-1 i EHV-4 u populaciji konja na određenom prostoru, primenjuju se serološke metode kao što su ELISA ili test virus neutralizacije. Primenom navedenih metoda se vrši otkrivanje prisustva i titra specifčnih antitela protiv prethodno navedenih virusa u uzorcima krvnih seruma konja. Ovde treba napomenuti da su danas dostupni i komercijalni kitovi za izvođenje metode ELISA koji omogućavaju diferencijaciju specifčnih EHV-1 i EHV-4 antitela. Konjski herpesvirus 1 i konjski herpesvirus 4 se mogu izolovati na kulturama ćelija poreklom od konja, kao i na drugim ćelijskim linijama, a daju karakterističan citopatogeni efekat. Pored navedenih klasičnih virusoloških metoda, danas su u upotrebi i molekularne metode dijagnostike kao što su PCR ili real-time PCR. Ključni nalazi i zaključak. Lančana reakcija polimeraze (PCR) predstavlja pogodnu screening metodu za brzo i pouzdano otkrivanje prisustva virusa EHV-1 i EHV-4 u ispitivanim uzorcima poreklom od konja. Real-time PCR je osetljiva, specifčna i kvantitativna metoda čijom primenom se takođe može utvrditi prisustvo navedenih virusa u organizmu konja. Metodom sekvenciranja genoma virusa EHV-1 i EHV-4 se mogu utvrditi sličnosti ili razlike između pojedinih sojeva virusa, odnosno može se izvršiti molekularna karakterizacija sojeva virusa EHV-1 i EHV-4 dominantno prisutnih u populaciji konja na određenoj teritoriji. .",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Standard and molecular methods in the diagnostics of infections caused by equine herpesviruses 1 and 4, Standardne i molekularne metode dijagnostike infekcija izazvanih konjskim herpesvirusima 1 i 4",
volume = "72",
number = "2",
pages = "68-79",
doi = "10.2298/VETGL170227002M"
}

Milić, N., Radalj, A.,& Nišavić, J.. (2018). Standard and molecular methods in the diagnostics of infections caused by equine herpesviruses 1 and 4. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 72(2), 68-79.
https://doi.org/10.2298/VETGL170227002M

Milić N, Radalj A, Nišavić J. Standard and molecular methods in the diagnostics of infections caused by equine herpesviruses 1 and 4. in Veterinarski Glasnik. 2018;72(2):68-79.
doi:10.2298/VETGL170227002M .

Milić, Nenad, Radalj, Andrea, Nišavić, Jakov, "Standard and molecular methods in the diagnostics of infections caused by equine herpesviruses 1 and 4" in Veterinarski Glasnik, 72, no. 2 (2018):68-79,
https://doi.org/10.2298/VETGL170227002M . .

TY  - JOUR
AU  - Radalj, Andrea
AU  - Nišavić, Jakov
AU  - Krnjaić, Dejan
AU  - Valčić, Miroslav
AU  - Jovanović, Tanja
AU  - Veljović, Ljubiša
AU  - Milić, Nenad
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1626
AB  - The presence of equine herpesviruses 1, 2 and 5 (EHV-1, EHV-2 and EHV-5) was examined in 66 samples of spinal cord, submandibular lymph nodes and spleen of healthy, non-vaccinated abattoir horses from different locations in the Republic of Serbia. Virus isolation was conducted on RK-13 cell line with the confirmation of isolated viral strains by multiplex nested polymerase chain reaction. The cytopathic effect was observed 48-72 h after the first inoculation in 28 (42.4%) organ samples, and after 5 days in 11 other samples (16.7%) that were all confirmed as EHV-1. Four other samples (6.1%) that showed cytopathic effects on day 5 of the third passage were all positive for EHV-5. Additionally, EHV-1, EHV-2, and EHV-5 were directly detected in all organs by multiplex nested PCR in 46 (69.7%), 3 (4.5%), and 7 (10.6%) samples, respectively. The molecular characterization based on nucleotide sequencing of the part of the gB gene showed that Serbian EHV-1 isolates were 100% homogenous and clustered with EHV-1 strains from Turkey, the United Kingdom, the United States, and Japan. The EHV-2 strain from Serbia branched together with Turkish EHV-2 isolates with homogeneity from 96% to 98%. Serbian EHV-5 strains can be separated in one distinct cluster with isolates from Turkey and the United States with homogeneity from 98 to 99%. These data represent the first report of the molecular characterization of EHV-1, EHV-2, and EHV-5 in the horse population of the Republic of Serbia and document the first successful isolation of Serbian EHV-5 strains.
PB  - Veterinarni A Farmaceuticka Univerzita Brno, Brno
T2  - Acta Veterinaria - Brno
T1  - Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia
VL  - 87
IS  - 1
SP  - 27
DO  - 10.2754/avb201887010027
ER  - 

@article{
author = "Radalj, Andrea and Nišavić, Jakov and Krnjaić, Dejan and Valčić, Miroslav and Jovanović, Tanja and Veljović, Ljubiša and Milić, Nenad",
year = "2018",
abstract = "The presence of equine herpesviruses 1, 2 and 5 (EHV-1, EHV-2 and EHV-5) was examined in 66 samples of spinal cord, submandibular lymph nodes and spleen of healthy, non-vaccinated abattoir horses from different locations in the Republic of Serbia. Virus isolation was conducted on RK-13 cell line with the confirmation of isolated viral strains by multiplex nested polymerase chain reaction. The cytopathic effect was observed 48-72 h after the first inoculation in 28 (42.4%) organ samples, and after 5 days in 11 other samples (16.7%) that were all confirmed as EHV-1. Four other samples (6.1%) that showed cytopathic effects on day 5 of the third passage were all positive for EHV-5. Additionally, EHV-1, EHV-2, and EHV-5 were directly detected in all organs by multiplex nested PCR in 46 (69.7%), 3 (4.5%), and 7 (10.6%) samples, respectively. The molecular characterization based on nucleotide sequencing of the part of the gB gene showed that Serbian EHV-1 isolates were 100% homogenous and clustered with EHV-1 strains from Turkey, the United Kingdom, the United States, and Japan. The EHV-2 strain from Serbia branched together with Turkish EHV-2 isolates with homogeneity from 96% to 98%. Serbian EHV-5 strains can be separated in one distinct cluster with isolates from Turkey and the United States with homogeneity from 98 to 99%. These data represent the first report of the molecular characterization of EHV-1, EHV-2, and EHV-5 in the horse population of the Republic of Serbia and document the first successful isolation of Serbian EHV-5 strains.",
publisher = "Veterinarni A Farmaceuticka Univerzita Brno, Brno",
journal = "Acta Veterinaria - Brno",
title = "Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia",
volume = "87",
number = "1",
pages = "27",
doi = "10.2754/avb201887010027"
}

Radalj, A., Nišavić, J., Krnjaić, D., Valčić, M., Jovanović, T., Veljović, L.,& Milić, N.. (2018). Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia. in Acta Veterinaria - Brno
Veterinarni A Farmaceuticka Univerzita Brno, Brno., 87(1), 27.
https://doi.org/10.2754/avb201887010027

Radalj A, Nišavić J, Krnjaić D, Valčić M, Jovanović T, Veljović L, Milić N. Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia. in Acta Veterinaria - Brno. 2018;87(1):27.
doi:10.2754/avb201887010027 .

Radalj, Andrea, Nišavić, Jakov, Krnjaić, Dejan, Valčić, Miroslav, Jovanović, Tanja, Veljović, Ljubiša, Milić, Nenad, "Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia" in Acta Veterinaria - Brno, 87, no. 1 (2018):27,
https://doi.org/10.2754/avb201887010027 . .

TY  - JOUR
AU  - Nišavić, Jakov
AU  - Knežević, Aleksandra
AU  - Stanojević, M.
AU  - Milić, Nenad
AU  - Radalj, Andrea
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1645
AB  - The presence of bovine herpesvirus 1 (BoHV-1) was examined in 110 samples of bovine nasal swabs collected between October 2007 and November 2015. BoHV-1 was detected in four samples by virus isolation and polymerase chain reaction (PCR). The phylogenetic analysis of glycoprotein B (gB) gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar to each other and branched with BoHV-1 strains isolated in Egypt and USA. BoHV-1 strains isolated in Israel, India, Brasil and USA belonging to the second branch were 100% similar to each other and 98% to 99% similar to the strains from Serbia. The phylogenetic analysis of the tk gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar and grouped together in one branch. BoHV-1 strains from USA and Australia clustered with Serbian BoHV-1 strains sharing similarity of 99%. BoHV-1 strains from India and USA clustered separately. The presented work is one of the first reports of the phylogenetic analysis of BoHV-1 isolates from Serbia.
PB  - Ecole Nationale Veterinaire Toulouse, Toulouse Cedex 3
T2  - Revue De Medecine Veterinaire
T1  - Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia
VL  - 169
IS  - 7-9
SP  - 180
EP  - 184
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1645
ER  - 

@article{
author = "Nišavić, Jakov and Knežević, Aleksandra and Stanojević, M. and Milić, Nenad and Radalj, Andrea",
year = "2018",
abstract = "The presence of bovine herpesvirus 1 (BoHV-1) was examined in 110 samples of bovine nasal swabs collected between October 2007 and November 2015. BoHV-1 was detected in four samples by virus isolation and polymerase chain reaction (PCR). The phylogenetic analysis of glycoprotein B (gB) gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar to each other and branched with BoHV-1 strains isolated in Egypt and USA. BoHV-1 strains isolated in Israel, India, Brasil and USA belonging to the second branch were 100% similar to each other and 98% to 99% similar to the strains from Serbia. The phylogenetic analysis of the tk gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar and grouped together in one branch. BoHV-1 strains from USA and Australia clustered with Serbian BoHV-1 strains sharing similarity of 99%. BoHV-1 strains from India and USA clustered separately. The presented work is one of the first reports of the phylogenetic analysis of BoHV-1 isolates from Serbia.",
publisher = "Ecole Nationale Veterinaire Toulouse, Toulouse Cedex 3",
journal = "Revue De Medecine Veterinaire",
title = "Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia",
volume = "169",
number = "7-9",
pages = "180-184",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1645"
}

Nišavić, J., Knežević, A., Stanojević, M., Milić, N.,& Radalj, A.. (2018). Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia. in Revue De Medecine Veterinaire
Ecole Nationale Veterinaire Toulouse, Toulouse Cedex 3., 169(7-9), 180-184.
https://hdl.handle.net/21.15107/rcub_veterinar_1645

Nišavić J, Knežević A, Stanojević M, Milić N, Radalj A. Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia. in Revue De Medecine Veterinaire. 2018;169(7-9):180-184.
https://hdl.handle.net/21.15107/rcub_veterinar_1645 .

Nišavić, Jakov, Knežević, Aleksandra, Stanojević, M., Milić, Nenad, Radalj, Andrea, "Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia" in Revue De Medecine Veterinaire, 169, no. 7-9 (2018):180-184,
https://hdl.handle.net/21.15107/rcub_veterinar_1645 .

TY  - THES
AU  - Radalj, Andrea
PY  - 2018
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50763279
UR  - http://nardus.mpn.gov.rs/123456789/10285
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/48
AB  - The aim of this PhD thesis was the identification and molecular characterization of equine herpesviruses from horses originating from the Republic of Serbia and The Republic of Srpska (Bosnia and Herzegovina), as well as archivated EHV-1 strains in the possession of The Department of Microbiology, Faculty of Veterinary Medicine, University of Belgrade. Archivated EHV-1 strains were isolated during abortion storms that occured on „Ljubičevo“ stud farm during the 1980s. Samples originating from 137 unvaccinated horses from stud farms and private breeders, i.e. 112 nasal swab samples and 100 organ samples from 25 horses (submandibular lymph nodes, spleen, spinal cord and medulla) were examined for the presence of equine herpesviruses 1, 4, 2 and 5 (EHV-1, EHV-4, EHV-2 and EHV-5) using standard and molecular virological methods. Archivated EHV-1 samples were examined by molecular methods in order to perform their genetic characterization. Equine herpesvirus 1 was isolated and identified by virus neutralization test, direct immunofluorescence and Nested multiplex PCR in 72 samples. Equine herpesvirus 5 was isolated from 7 examined samples and identified by Nested multiplex PCR, whilst the isolation of EHV-4 and EHV-2 was not successful. The direct examination of the presence of equine herpesviruses 1, 4, 2 and 5 was performed by Nested multiplex PCR directly from the samples of organs and nasal swabs and confirmed 162 positive samples of which 16.67% accounted for mixed infections with multiple equine herpesviruses. Equine herpesvirus 1 was confirmed in 153 samples, EHV-4 in 11, EHV-2 in 4 and EHV-5 in 23 samples, whilst EHV-2 and EHV-4 were only detected in mixed infections with EHV-1 and/or EHV-5. Partial gB gene nucleotide sequences of identified EHV-1 strains were 98 to 100% homologous amongst each other and with sequences from GenBank whilst their phylogenetic analysis showed grouping with strains from Turkey, UK, USA and Japan...
AB  - Cilj ove doktorske disertacije je identifikacija i molekularna karakterizacija sojeva herpesvirusa konja poreklom iz uzoraka od konja sa teritorije Republike Srbije i Republike Srpske (BiH) kao i arhiviranih sojeva EHV-1 Katedre za mikrobiologiju Fakulteta veterinarske medicine Univerziteta u Beogradu. Arhivirani sojevi EHV-1 su liofilizati pomenutog virusa izolovani osamdesetih godina prošlog veka iz uzoraka pobačenih fetusa konja, kao i iz uginule novorođene ždrebadi sa ergele „Ljubičevo“. Ispitivani su uzorci poreklom od ukupno 137 nevakcinisanih konja sa ergela i iz privatnog sektora koji su obuhvatali 112 uzoraka nosnih briseva i 100 uzoraka organa - submandibularnih limfnih čvorova, slezine, produžene moždine i kičmene moždine prikupljenih od 25 konja. Prikupljeni uzorci nosnih briseva i organa konja su primenom klasičnih i molekularnih metoda virusološke dijagnostike ispitivani na prisustvo konjskih herpesvirusa 1, 4, 2 i 5 (EHV-1, EHV-4, EHV-2 i EHV-5), dok su arhivirani sojevi ispitivani primenom molekularnih metoda u cilju molekularne karakterizacije. Posle izolacije i identifikacije virusa na kulturi tkiva primenom testa virus neutralizacije, direktne imunofluorescencije i metode Nested multiplex PCR od ukupno 212 ispitivanih uzoraka organa i nosnih briseva konja prisustvo konjskog herpesvirusa 1 je utvrđeno u 72 uzorka, dok je metodom izolacije virusa na kulturi ćelija sa identifikacijom primenom metode Nested multiplex PCR iz prethodno navedenih uzoraka konjski herpesvirus 5 detektovan u 7 uzoraka, a konjski herpesvirusi 4 i 2 nisu izolovani ni iz jednog uzorka nosnih briseva i organa konja. Primenom metode Nested multiplex PCR za ispitivanje prisustva nukleinskih kiselina konjskih herpesvirusa 1, 4, 2 i 5 direktno u uzorcima ispitivanog materijala (organa i nosnih briseva konja) detektovano je ukupno 162 uzorka pri čemu je EHV-1 detektovan u 153 uzorka, EHV-4 u 11, EHV-2 u 4, a EHV-5 u 23 uzorka ispitivanog materijala. Od ukupnog broja pozitivnih uzoraka mešovite infekcije sa dva ili više konjska herpesvirusa utvrđene su u 16,67% uzoraka, pri čemu su EHV-2 i EHV-4 identifikovani isključivo u okviru mešovitih infekcija sa EHV-1 i/ili EHV-5...
PB  - Univerzitet u Beogradu, Fakultet veterinarske medicine
T1  - Identification and molecular characterization of equine herpesviruses
T1  - Identifikacija i molekularna karakterizacija herpesvirusa konja
UR  - https://hdl.handle.net/21.15107/rcub_nardus_10285
ER  - 

@phdthesis{
author = "Radalj, Andrea",
year = "2018",
abstract = "The aim of this PhD thesis was the identification and molecular characterization of equine herpesviruses from horses originating from the Republic of Serbia and The Republic of Srpska (Bosnia and Herzegovina), as well as archivated EHV-1 strains in the possession of The Department of Microbiology, Faculty of Veterinary Medicine, University of Belgrade. Archivated EHV-1 strains were isolated during abortion storms that occured on „Ljubičevo“ stud farm during the 1980s. Samples originating from 137 unvaccinated horses from stud farms and private breeders, i.e. 112 nasal swab samples and 100 organ samples from 25 horses (submandibular lymph nodes, spleen, spinal cord and medulla) were examined for the presence of equine herpesviruses 1, 4, 2 and 5 (EHV-1, EHV-4, EHV-2 and EHV-5) using standard and molecular virological methods. Archivated EHV-1 samples were examined by molecular methods in order to perform their genetic characterization. Equine herpesvirus 1 was isolated and identified by virus neutralization test, direct immunofluorescence and Nested multiplex PCR in 72 samples. Equine herpesvirus 5 was isolated from 7 examined samples and identified by Nested multiplex PCR, whilst the isolation of EHV-4 and EHV-2 was not successful. The direct examination of the presence of equine herpesviruses 1, 4, 2 and 5 was performed by Nested multiplex PCR directly from the samples of organs and nasal swabs and confirmed 162 positive samples of which 16.67% accounted for mixed infections with multiple equine herpesviruses. Equine herpesvirus 1 was confirmed in 153 samples, EHV-4 in 11, EHV-2 in 4 and EHV-5 in 23 samples, whilst EHV-2 and EHV-4 were only detected in mixed infections with EHV-1 and/or EHV-5. Partial gB gene nucleotide sequences of identified EHV-1 strains were 98 to 100% homologous amongst each other and with sequences from GenBank whilst their phylogenetic analysis showed grouping with strains from Turkey, UK, USA and Japan..., Cilj ove doktorske disertacije je identifikacija i molekularna karakterizacija sojeva herpesvirusa konja poreklom iz uzoraka od konja sa teritorije Republike Srbije i Republike Srpske (BiH) kao i arhiviranih sojeva EHV-1 Katedre za mikrobiologiju Fakulteta veterinarske medicine Univerziteta u Beogradu. Arhivirani sojevi EHV-1 su liofilizati pomenutog virusa izolovani osamdesetih godina prošlog veka iz uzoraka pobačenih fetusa konja, kao i iz uginule novorođene ždrebadi sa ergele „Ljubičevo“. Ispitivani su uzorci poreklom od ukupno 137 nevakcinisanih konja sa ergela i iz privatnog sektora koji su obuhvatali 112 uzoraka nosnih briseva i 100 uzoraka organa - submandibularnih limfnih čvorova, slezine, produžene moždine i kičmene moždine prikupljenih od 25 konja. Prikupljeni uzorci nosnih briseva i organa konja su primenom klasičnih i molekularnih metoda virusološke dijagnostike ispitivani na prisustvo konjskih herpesvirusa 1, 4, 2 i 5 (EHV-1, EHV-4, EHV-2 i EHV-5), dok su arhivirani sojevi ispitivani primenom molekularnih metoda u cilju molekularne karakterizacije. Posle izolacije i identifikacije virusa na kulturi tkiva primenom testa virus neutralizacije, direktne imunofluorescencije i metode Nested multiplex PCR od ukupno 212 ispitivanih uzoraka organa i nosnih briseva konja prisustvo konjskog herpesvirusa 1 je utvrđeno u 72 uzorka, dok je metodom izolacije virusa na kulturi ćelija sa identifikacijom primenom metode Nested multiplex PCR iz prethodno navedenih uzoraka konjski herpesvirus 5 detektovan u 7 uzoraka, a konjski herpesvirusi 4 i 2 nisu izolovani ni iz jednog uzorka nosnih briseva i organa konja. Primenom metode Nested multiplex PCR za ispitivanje prisustva nukleinskih kiselina konjskih herpesvirusa 1, 4, 2 i 5 direktno u uzorcima ispitivanog materijala (organa i nosnih briseva konja) detektovano je ukupno 162 uzorka pri čemu je EHV-1 detektovan u 153 uzorka, EHV-4 u 11, EHV-2 u 4, a EHV-5 u 23 uzorka ispitivanog materijala. Od ukupnog broja pozitivnih uzoraka mešovite infekcije sa dva ili više konjska herpesvirusa utvrđene su u 16,67% uzoraka, pri čemu su EHV-2 i EHV-4 identifikovani isključivo u okviru mešovitih infekcija sa EHV-1 i/ili EHV-5...",
publisher = "Univerzitet u Beogradu, Fakultet veterinarske medicine",
title = "Identification and molecular characterization of equine herpesviruses, Identifikacija i molekularna karakterizacija herpesvirusa konja",
url = "https://hdl.handle.net/21.15107/rcub_nardus_10285"
}

Radalj, A.. (2018). Identification and molecular characterization of equine herpesviruses. 
Univerzitet u Beogradu, Fakultet veterinarske medicine..
https://hdl.handle.net/21.15107/rcub_nardus_10285

Radalj A. Identification and molecular characterization of equine herpesviruses. 2018;.
https://hdl.handle.net/21.15107/rcub_nardus_10285 .

Radalj, Andrea, "Identification and molecular characterization of equine herpesviruses" (2018),
https://hdl.handle.net/21.15107/rcub_nardus_10285 .

TY  - JOUR
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Zorić, Andrea
AU  - Krnjaić, Dejan
AU  - Radojičić, Marina
AU  - Stanojković, Aleksandar
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1453
AB  - Newcastle disease virus (NDV) is one of the most important viral pathogens of avian species and the causative agent of atypical fowl plague, a highly contagious and economically important disease characterized by high mortality rates and reduction of egg production. The HN and F proteins are the main targets for immune response to NDV. Vaccination of poultry with live and inactivated NDV vaccines is the most effective method of control and prevention of Newcastle disease, however due to their disadvantages, efforts are being invested into developing subunit vaccines. To this end, the NDV HN and/or F protein have been expressed using different viruses as vectors, but have also been expressed using transgenic plant systems, yeast and lactic acid bacteria in order to produce the NDV subunit vaccine. Many authors have investigated the possibility of preparation of vaccines from purified and biologically active NDV subunits with HN and F glycoproteins, purified from nucleocapsids, viral ribonucleic acid (RNA) and pyrogens. The above mentioned viral glycoproteins with preserved antigenic structure and biological activities can be used as subunit vaccinal antigens due to their immunogenic properties.
AB  - Virus Newcastle bolesti je jedan od najznačajnijih patogena u populaciji ptica i domaće živine koji izaziva atipičnu kugu živine, kontagiozno oboljenje koje prati visoka stopa morbiditeta i mortaliteta, što ima za posledicu i velike ekonomske gubitke u živinarstvu. Glikoproteinski HN i F antigeni virusa atipične kuge živine su najznačajniji prilikom razvoja imunološkog odgovora prijemčivih jedinki. Vakcinacija živine živim i inaktivisanim vakcinama protiv virusa Newcastle bolesti predstavlja najefikasniji metod kontrole i prevencije navedenog oboljenja, međutim klasične vakcine imaju izvesne nedostatke i iz tog razloga se sve više istraživanja se usmerava na razvoj subjediničnih vakcina. U cilju razvoja subjediničnih vakcina u današnje vreme se za ekspresiju HN i F proteina virusa Newcastle bolesti koriste različiti vektori kao što su virusi, transgene biljke, kvasci i mlečnokiselinske bakterije. Pored toga, mnogi autori su ispitivali mogućnosti pripremanja subjediničnih vakcina od prečišćenih i biološki aktivnih subjedinica, odnosno HN i F glikoproteina pomenutog virusa, oslobođenih od nukleokapsida sa virusnom ribonukleinskom kiselinom (RNK) i pirogena. Virusni glikoproteini sa očuvanom antigenskom strukturom i biološkim aktivnostima se zbog svojih imunogenih svojstava mogu koristiti kao subjedinični vakcinalni antigeni.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus
T1  - Pregled savremenih saznanja o razvoju subjediničnih i rekombinantnih vakcina protiv virusa Newcastle bolesti živine
VL  - 33
IS  - 1
SP  - 1
EP  - 11
DO  - 10.2298/BAH1701001M
ER  - 

@article{
author = "Milić, Nenad and Nišavić, Jakov and Zorić, Andrea and Krnjaić, Dejan and Radojičić, Marina and Stanojković, Aleksandar",
year = "2017",
abstract = "Newcastle disease virus (NDV) is one of the most important viral pathogens of avian species and the causative agent of atypical fowl plague, a highly contagious and economically important disease characterized by high mortality rates and reduction of egg production. The HN and F proteins are the main targets for immune response to NDV. Vaccination of poultry with live and inactivated NDV vaccines is the most effective method of control and prevention of Newcastle disease, however due to their disadvantages, efforts are being invested into developing subunit vaccines. To this end, the NDV HN and/or F protein have been expressed using different viruses as vectors, but have also been expressed using transgenic plant systems, yeast and lactic acid bacteria in order to produce the NDV subunit vaccine. Many authors have investigated the possibility of preparation of vaccines from purified and biologically active NDV subunits with HN and F glycoproteins, purified from nucleocapsids, viral ribonucleic acid (RNA) and pyrogens. The above mentioned viral glycoproteins with preserved antigenic structure and biological activities can be used as subunit vaccinal antigens due to their immunogenic properties., Virus Newcastle bolesti je jedan od najznačajnijih patogena u populaciji ptica i domaće živine koji izaziva atipičnu kugu živine, kontagiozno oboljenje koje prati visoka stopa morbiditeta i mortaliteta, što ima za posledicu i velike ekonomske gubitke u živinarstvu. Glikoproteinski HN i F antigeni virusa atipične kuge živine su najznačajniji prilikom razvoja imunološkog odgovora prijemčivih jedinki. Vakcinacija živine živim i inaktivisanim vakcinama protiv virusa Newcastle bolesti predstavlja najefikasniji metod kontrole i prevencije navedenog oboljenja, međutim klasične vakcine imaju izvesne nedostatke i iz tog razloga se sve više istraživanja se usmerava na razvoj subjediničnih vakcina. U cilju razvoja subjediničnih vakcina u današnje vreme se za ekspresiju HN i F proteina virusa Newcastle bolesti koriste različiti vektori kao što su virusi, transgene biljke, kvasci i mlečnokiselinske bakterije. Pored toga, mnogi autori su ispitivali mogućnosti pripremanja subjediničnih vakcina od prečišćenih i biološki aktivnih subjedinica, odnosno HN i F glikoproteina pomenutog virusa, oslobođenih od nukleokapsida sa virusnom ribonukleinskom kiselinom (RNK) i pirogena. Virusni glikoproteini sa očuvanom antigenskom strukturom i biološkim aktivnostima se zbog svojih imunogenih svojstava mogu koristiti kao subjedinični vakcinalni antigeni.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus, Pregled savremenih saznanja o razvoju subjediničnih i rekombinantnih vakcina protiv virusa Newcastle bolesti živine",
volume = "33",
number = "1",
pages = "1-11",
doi = "10.2298/BAH1701001M"
}

Milić, N., Nišavić, J., Zorić, A., Krnjaić, D., Radojičić, M.,& Stanojković, A.. (2017). Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 33(1), 1-11.
https://doi.org/10.2298/BAH1701001M

Milić N, Nišavić J, Zorić A, Krnjaić D, Radojičić M, Stanojković A. Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus. in Biotechnology in Animal Husbandry. 2017;33(1):1-11.
doi:10.2298/BAH1701001M .

Milić, Nenad, Nišavić, Jakov, Zorić, Andrea, Krnjaić, Dejan, Radojičić, Marina, Stanojković, Aleksandar, "Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus" in Biotechnology in Animal Husbandry, 33, no. 1 (2017):1-11,
https://doi.org/10.2298/BAH1701001M . .

TY  - JOUR
AU  - Nišavić, Jakov
AU  - Milić, Nenad
AU  - Zorić, Andrea
AU  - Bojkovski, Jovan
AU  - Stanojković, Aleksandar
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1320
AB  - Viral infections of swine cause significant economic losses in swine husbandry. They manifest in death of infected animals of different ages or in decreased productivity during the manufacturing process. Having that in mind, rapid and reliable diagnostics of viral infections is crucial in the prevention of disease transmission in herds of swine. Today, virological laboratories all over the world use different diagnostic methods such as isolation of virus in cell lines, ELISA, virus neutralization test, direct and indirect immunofluorescence and hemagglutination and hemagglutination inhibition tests. Virus isolation, virus neutralization test and some other standard virological methods are time consuming and rather expensive, therefore, molecular methods such as conventional PCR, RT - PCR, real-time PCR and direct sequencing methods are applied worldwide as fast and reliable. Their application is especially necessary for the detection of viruses which cannot be identified by using standard virological methods.
AB  - Virusne infekcije izazivaju značajne ekonomske gubitke u svinjarskoj proizvodnji. One se ispoljavaju kako kroz pojavu uginuća životinja, tako i kroz smanjenje produktivnosti. U cilju otkrivanja i sprečavanja širenja virusnih oboljenja svinja danas se u svetu primenjuju standardne i molekularne metode virusološke dijagnostike. Od standardnih metoda dijagnostike u upotrebi su metode izolacije virusa u kulturi ćelija, zatim ELISA, direktna i indirektna imunofluorescencija, kao i hemaglutinacija i inhibicija hemaglutinacije. Primena navedenih metoda podrazumeva duže vreme potrebno za dobijanje rezultata ispitivanja od najmanje 5 do 7 dana. Međutim, primena savremenih molekularnih metoda virusološke dijagnostike kao što su PCR, real-time PCR, odnosno metoda direktnog sekvenciranja, podrazumeva kraće vreme potrebno za dobijanje rezultata, odnosno omogućava preciznu dijagnostiku oboljenja u kraćem vremenskom periodu. Pored ovoga, značaj primene ovih metoda se ogleda i u otkrivanju virusa čije se prisustvo u uzorcima na drugi način, odnosno primenom standardnih metoda virusološke dijagnostike ne može detektovati.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - The application of PCR based methods in diagnostics of some viral infections of swine
T1  - Primena molekularnih metoda zasnovanih na lančanoj reakciji polimeraze u dijagnostici nekih infekcija svinja
VL  - 32
IS  - 4
SP  - 321
EP  - 329
DO  - 10.2298/bah1604321N
ER  - 

@article{
author = "Nišavić, Jakov and Milić, Nenad and Zorić, Andrea and Bojkovski, Jovan and Stanojković, Aleksandar",
year = "2016",
abstract = "Viral infections of swine cause significant economic losses in swine husbandry. They manifest in death of infected animals of different ages or in decreased productivity during the manufacturing process. Having that in mind, rapid and reliable diagnostics of viral infections is crucial in the prevention of disease transmission in herds of swine. Today, virological laboratories all over the world use different diagnostic methods such as isolation of virus in cell lines, ELISA, virus neutralization test, direct and indirect immunofluorescence and hemagglutination and hemagglutination inhibition tests. Virus isolation, virus neutralization test and some other standard virological methods are time consuming and rather expensive, therefore, molecular methods such as conventional PCR, RT - PCR, real-time PCR and direct sequencing methods are applied worldwide as fast and reliable. Their application is especially necessary for the detection of viruses which cannot be identified by using standard virological methods., Virusne infekcije izazivaju značajne ekonomske gubitke u svinjarskoj proizvodnji. One se ispoljavaju kako kroz pojavu uginuća životinja, tako i kroz smanjenje produktivnosti. U cilju otkrivanja i sprečavanja širenja virusnih oboljenja svinja danas se u svetu primenjuju standardne i molekularne metode virusološke dijagnostike. Od standardnih metoda dijagnostike u upotrebi su metode izolacije virusa u kulturi ćelija, zatim ELISA, direktna i indirektna imunofluorescencija, kao i hemaglutinacija i inhibicija hemaglutinacije. Primena navedenih metoda podrazumeva duže vreme potrebno za dobijanje rezultata ispitivanja od najmanje 5 do 7 dana. Međutim, primena savremenih molekularnih metoda virusološke dijagnostike kao što su PCR, real-time PCR, odnosno metoda direktnog sekvenciranja, podrazumeva kraće vreme potrebno za dobijanje rezultata, odnosno omogućava preciznu dijagnostiku oboljenja u kraćem vremenskom periodu. Pored ovoga, značaj primene ovih metoda se ogleda i u otkrivanju virusa čije se prisustvo u uzorcima na drugi način, odnosno primenom standardnih metoda virusološke dijagnostike ne može detektovati.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "The application of PCR based methods in diagnostics of some viral infections of swine, Primena molekularnih metoda zasnovanih na lančanoj reakciji polimeraze u dijagnostici nekih infekcija svinja",
volume = "32",
number = "4",
pages = "321-329",
doi = "10.2298/bah1604321N"
}

Nišavić, J., Milić, N., Zorić, A., Bojkovski, J.,& Stanojković, A.. (2016). The application of PCR based methods in diagnostics of some viral infections of swine. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 32(4), 321-329.
https://doi.org/10.2298/bah1604321N

Nišavić J, Milić N, Zorić A, Bojkovski J, Stanojković A. The application of PCR based methods in diagnostics of some viral infections of swine. in Biotechnology in Animal Husbandry. 2016;32(4):321-329.
doi:10.2298/bah1604321N .

Nišavić, Jakov, Milić, Nenad, Zorić, Andrea, Bojkovski, Jovan, Stanojković, Aleksandar, "The application of PCR based methods in diagnostics of some viral infections of swine" in Biotechnology in Animal Husbandry, 32, no. 4 (2016):321-329,
https://doi.org/10.2298/bah1604321N . .

TY  - JOUR
AU  - Lukač, Bojan
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Veljović, Ljubiša
AU  - Milićević, Vesna
AU  - Zorić, Andrea
AU  - Đurić, Spomenka
AU  - Stanojević, Maja
AU  - Nišavić, Jakov
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1352
AB  - The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (15%), while porcine parvovirus DNA was identified in five lymph node samples (12.5%). The presence of both viruses was detected in three lymph node samples (7.5%). Partial nucleotide sequence of ORF1 gene of 2 porcine circovirus 2 and VP2 gene of 2 porcine parvovirus isolates was determined. The nucleotide sequences of two PCV2 isolates from RS-BIH included in phylogenetic typing are similar and cluster together with the strain Mantova isolated from domestic pigs in Italy, strains DE006-14 and DE222-13 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar to the strain NIV-C SRB isolated from pigs in Serbia. The nucleotide sequences of two PPV isolates that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China.
AB  - Prisustvo svinjskog cirkovirusa 2 i parvovirusa svinja ispitano je u četrdeset uzoraka (slezina, limfni čvorovi, pluća) poreklom od nevakcinisanih svinja primenom lančane reakcije polimeraze. Sve životinje su bile iz ekstenzivnog načina gajenja i iz različitih regiona Republike Srpske, BiH. Četiri uzorka limfnih čvorova i dva uzorka slezine su bili pozitivni na prisustvo DNK svinjskog cirkovirusa 2 (15%), dok je kod pet uzoraka limfnih čvorova utvrđeno prisustvo DNK parvovirusa svinja (12.5%). U uzorcima poreklom od tri svinje utvrđeno je prisustvo nukleinske kiseline oba prethodno navedena virusa (7.5%). Metodom sekvenciranja određena je nukleotidna sekvenca dela ORF1 gena dva izolata svinjskog cirkovirusa 2 i dela VP2 gena dva izolata parvovirusa svinja. Nukleotidne sekvence dva izolata PCV2 utvrđena u uzorcima svinja poreklom iz RS-BiH koja su bila uključena u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama soja Mantova izolovanog kod svinja u Italiji, zatim sojeva DE006-14 i DE222-13 izolovanih kod svinja u Nemačkoj kao i sa sojem Jvnan izolovanog kod svinja u Kini. Istovremeno, izolati PCV2 utvrđeni kod svinja u RS-BiH su bili delimično slični sa sojem NIV-C SRB virusa PCV2 izolovanim kod svinja u Srbiji. Nukleotidne sekvence dva izolata parvovirusa svinja uključenih u filogenetsku analizu su pokazale visok stepen sličnosti sa sojem Challenge izolovanim kod svinja u UK, sojem Kresse izolovanim kod svinja u SAD-u kao i sojevima 77 i LZ izolovanim kod svinja u Kini.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina
T1  - Molekularna detekcija PCV2 i PPV kod svinja u Republici Srpskoj, Bosna i Hercegovina
VL  - 66
IS  - 1
SP  - 51
EP  - 60
DO  - 10.1515/acve-2016-0004
ER  - 

@article{
author = "Lukač, Bojan and Knežević, Aleksandra and Milić, Nenad and Krnjaić, Dejan and Veljović, Ljubiša and Milićević, Vesna and Zorić, Andrea and Đurić, Spomenka and Stanojević, Maja and Nišavić, Jakov",
year = "2016",
abstract = "The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (15%), while porcine parvovirus DNA was identified in five lymph node samples (12.5%). The presence of both viruses was detected in three lymph node samples (7.5%). Partial nucleotide sequence of ORF1 gene of 2 porcine circovirus 2 and VP2 gene of 2 porcine parvovirus isolates was determined. The nucleotide sequences of two PCV2 isolates from RS-BIH included in phylogenetic typing are similar and cluster together with the strain Mantova isolated from domestic pigs in Italy, strains DE006-14 and DE222-13 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar to the strain NIV-C SRB isolated from pigs in Serbia. The nucleotide sequences of two PPV isolates that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China., Prisustvo svinjskog cirkovirusa 2 i parvovirusa svinja ispitano je u četrdeset uzoraka (slezina, limfni čvorovi, pluća) poreklom od nevakcinisanih svinja primenom lančane reakcije polimeraze. Sve životinje su bile iz ekstenzivnog načina gajenja i iz različitih regiona Republike Srpske, BiH. Četiri uzorka limfnih čvorova i dva uzorka slezine su bili pozitivni na prisustvo DNK svinjskog cirkovirusa 2 (15%), dok je kod pet uzoraka limfnih čvorova utvrđeno prisustvo DNK parvovirusa svinja (12.5%). U uzorcima poreklom od tri svinje utvrđeno je prisustvo nukleinske kiseline oba prethodno navedena virusa (7.5%). Metodom sekvenciranja određena je nukleotidna sekvenca dela ORF1 gena dva izolata svinjskog cirkovirusa 2 i dela VP2 gena dva izolata parvovirusa svinja. Nukleotidne sekvence dva izolata PCV2 utvrđena u uzorcima svinja poreklom iz RS-BiH koja su bila uključena u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama soja Mantova izolovanog kod svinja u Italiji, zatim sojeva DE006-14 i DE222-13 izolovanih kod svinja u Nemačkoj kao i sa sojem Jvnan izolovanog kod svinja u Kini. Istovremeno, izolati PCV2 utvrđeni kod svinja u RS-BiH su bili delimično slični sa sojem NIV-C SRB virusa PCV2 izolovanim kod svinja u Srbiji. Nukleotidne sekvence dva izolata parvovirusa svinja uključenih u filogenetsku analizu su pokazale visok stepen sličnosti sa sojem Challenge izolovanim kod svinja u UK, sojem Kresse izolovanim kod svinja u SAD-u kao i sojevima 77 i LZ izolovanim kod svinja u Kini.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina, Molekularna detekcija PCV2 i PPV kod svinja u Republici Srpskoj, Bosna i Hercegovina",
volume = "66",
number = "1",
pages = "51-60",
doi = "10.1515/acve-2016-0004"
}

Lukač, B., Knežević, A., Milić, N., Krnjaić, D., Veljović, L., Milićević, V., Zorić, A., Đurić, S., Stanojević, M.,& Nišavić, J.. (2016). Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(1), 51-60.
https://doi.org/10.1515/acve-2016-0004

Lukač B, Knežević A, Milić N, Krnjaić D, Veljović L, Milićević V, Zorić A, Đurić S, Stanojević M, Nišavić J. Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina. in Acta Veterinaria-Beograd. 2016;66(1):51-60.
doi:10.1515/acve-2016-0004 .

Lukač, Bojan, Knežević, Aleksandra, Milić, Nenad, Krnjaić, Dejan, Veljović, Ljubiša, Milićević, Vesna, Zorić, Andrea, Đurić, Spomenka, Stanojević, Maja, Nišavić, Jakov, "Molecular detection of PCV2 and PPV in pigs in Republic of Srpska, Bosnia and Herzegovina" in Acta Veterinaria-Beograd, 66, no. 1 (2016):51-60,
https://doi.org/10.1515/acve-2016-0004 . .

TY  - JOUR
AU  - Miković, Radoš
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Radojičić, Marina
AU  - Veljović, Ljubiša
AU  - Milićević, Vesna
AU  - Zorić, Andrea
AU  - Stanojević, Maja
AU  - Nišavić, Jakov
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1359
AB  - The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identified in this study showed high similarity and significant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and significant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identified in swine in Republic of Montenegro. Besides that, these results confirmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine.
AB  - Primenom metode izolacije virusa i PCR metode, ukupno je ispitano devedeset uzoraka poreklom od svinja iz ekstenzivnog uzgoja iz različitih delova Crne Gore na prisustvo pseudorabijes virusa svinja (PrV), parvovirusa svinja (PPV) i svinjskog cirkovirusa 2 (PCV2). Primenom PCR metode prisustvo PrV je ustanovljeno kod tri uzorka, PPV kod sedam uzoraka, dok je prisustvo PCV2 utvrđeno kod šest uzoraka poreklom od svinja. Nukleotidne sekvence tri izolata PrV utvrđene u uzorcima poreklom od svinja koje su bile uključene u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama sojeva Kaplan i Bartha virusa izolovanih u Mađarskoj, zatim sa sekvencom soja Becker izolovanog u SAD-u i sekvencom soja Kolchis izolovanog u uzorcima poreklom od svinja u Grčkoj. Sva tri izolata su pripadala genotipu I pseudorabijes virusa. Nukleotidne sekvence dva izolata parvovirusa svinja su pokazale visok stepen sličnosti sa sekvencom soja Challenge izolovanog kod svinja u Velikoj Britaniji, zatim sekvencama soja Kresse izolovanog kod svinja u SAD-u i sojeva 77 i LZ izolovanih kod svinja u Kini. Filogenetska analiza nukleotidnih sekvenci dva PCV2 izolata je pokazala visok stepen sličnosti sa sojevima genotipa PCV2b kao što su NIVS-3, NIVS-5 i NIVS-6 izolovanim u Srbiji, sojem 3959 virusa izolovanim u Austriji i sojem PM165 virusa izolovanim u Brazilu i sojem XT2008 virusa izolovanim u Kini. Dobijeni rezultati ispitivanja pružili su uvid u molekularnu karakterizaciju sojeva virusa Aujeckijeve bolesti, svinjskog parvovirusa i svinjskog cirkovirusa 2 identifikovanih kod svinja u Crnoj Gori. Pored toga, dobijeni rezultati ispitivanja su potvrdili opravdanost korišćenja metode PCR u brzoj i pouzdanoj detekciji prethodno navedenih virusa kod supklinički inficiranih svinja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro
T1  - Molekularna detekcija PrV, PPV i PCV2 kod svinja u Republici Crnoj Gori
VL  - 66
IS  - 3
SP  - 347
EP  - 358
DO  - 10.1515/acve-2016-0030
ER  - 

@article{
author = "Miković, Radoš and Knežević, Aleksandra and Milić, Nenad and Krnjaić, Dejan and Radojičić, Marina and Veljović, Ljubiša and Milićević, Vesna and Zorić, Andrea and Stanojević, Maja and Nišavić, Jakov",
year = "2016",
abstract = "The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identified in this study showed high similarity and significant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and significant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identified in swine in Republic of Montenegro. Besides that, these results confirmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine., Primenom metode izolacije virusa i PCR metode, ukupno je ispitano devedeset uzoraka poreklom od svinja iz ekstenzivnog uzgoja iz različitih delova Crne Gore na prisustvo pseudorabijes virusa svinja (PrV), parvovirusa svinja (PPV) i svinjskog cirkovirusa 2 (PCV2). Primenom PCR metode prisustvo PrV je ustanovljeno kod tri uzorka, PPV kod sedam uzoraka, dok je prisustvo PCV2 utvrđeno kod šest uzoraka poreklom od svinja. Nukleotidne sekvence tri izolata PrV utvrđene u uzorcima poreklom od svinja koje su bile uključene u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama sojeva Kaplan i Bartha virusa izolovanih u Mađarskoj, zatim sa sekvencom soja Becker izolovanog u SAD-u i sekvencom soja Kolchis izolovanog u uzorcima poreklom od svinja u Grčkoj. Sva tri izolata su pripadala genotipu I pseudorabijes virusa. Nukleotidne sekvence dva izolata parvovirusa svinja su pokazale visok stepen sličnosti sa sekvencom soja Challenge izolovanog kod svinja u Velikoj Britaniji, zatim sekvencama soja Kresse izolovanog kod svinja u SAD-u i sojeva 77 i LZ izolovanih kod svinja u Kini. Filogenetska analiza nukleotidnih sekvenci dva PCV2 izolata je pokazala visok stepen sličnosti sa sojevima genotipa PCV2b kao što su NIVS-3, NIVS-5 i NIVS-6 izolovanim u Srbiji, sojem 3959 virusa izolovanim u Austriji i sojem PM165 virusa izolovanim u Brazilu i sojem XT2008 virusa izolovanim u Kini. Dobijeni rezultati ispitivanja pružili su uvid u molekularnu karakterizaciju sojeva virusa Aujeckijeve bolesti, svinjskog parvovirusa i svinjskog cirkovirusa 2 identifikovanih kod svinja u Crnoj Gori. Pored toga, dobijeni rezultati ispitivanja su potvrdili opravdanost korišćenja metode PCR u brzoj i pouzdanoj detekciji prethodno navedenih virusa kod supklinički inficiranih svinja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro, Molekularna detekcija PrV, PPV i PCV2 kod svinja u Republici Crnoj Gori",
volume = "66",
number = "3",
pages = "347-358",
doi = "10.1515/acve-2016-0030"
}

Miković, R., Knežević, A., Milić, N., Krnjaić, D., Radojičić, M., Veljović, L., Milićević, V., Zorić, A., Stanojević, M.,& Nišavić, J.. (2016). Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3), 347-358.
https://doi.org/10.1515/acve-2016-0030

Miković R, Knežević A, Milić N, Krnjaić D, Radojičić M, Veljović L, Milićević V, Zorić A, Stanojević M, Nišavić J. Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro. in Acta Veterinaria-Beograd. 2016;66(3):347-358.
doi:10.1515/acve-2016-0030 .

Miković, Radoš, Knežević, Aleksandra, Milić, Nenad, Krnjaić, Dejan, Radojičić, Marina, Veljović, Ljubiša, Milićević, Vesna, Zorić, Andrea, Stanojević, Maja, Nišavić, Jakov, "Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro" in Acta Veterinaria-Beograd, 66, no. 3 (2016):347-358,
https://doi.org/10.1515/acve-2016-0030 . .

TY  - JOUR
AU  - Veljović, Ljubiša
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Miković, Radoš
AU  - Zorić, Andrea
AU  - Marković, Maja
AU  - Milićević, Vesna
AU  - Stamenković, Miodrag
AU  - Stanojević, Maja
AU  - Maksimović-Zorić, Jelena
AU  - Petrović, Tamaš
AU  - Nišavić, Jakov
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1368
AB  - The presence of bovine parainfluenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight samples (8/119; 6.7%). The confirmation of isolated strains of BPIV3 was done by the virus-neutralization test. In addition, all samples of bovine nasal swabs were also examined for the presence of BPIV3 virus using RT-PCR with primers specific for the part of HN gene. The presence of BPIV3 was detected in eight samples (8/119; 6.7%) that were also positive upon virus isolation. The molecular characterization based on nucleotide sequencing of the part of the HN gene showed that all BPIV3 isolates belonged to genotype C of BPIV3. They branched in one distinct cluster with three different branches, but these branches were very similar to each other (98.1% to 99.8%). Serbian BPIV3c isolates were most similar to the Chinese BPIV3c isolates SD0805, SD0809 and SD0835 (from 97.92% to 99.7%), and to South Korean (12Q061), Japanese (HS9) and American (TVMDL16 and TVMDL20) BPIV3c strains (from 97.1% to 98.8%), and distinct from American (TVMDL15and TVMDL17) and Australian (Q5592) BPI3V genotype B strains (only 79.9% to 82.3% similarity), as well as from the genotype A BPIV3 strains from different countries published in GenBank.
AB  - Ukupno je ispitano 119 uzoraka nosnih briseva goveda na prisustvo parainfluenca 3 virusa goveda (bovine parainfluenza virus type, eng. - 3 BPIV3). Iz svih uzoraka nosnih briseva je vršena izolacija virusa na ćelijskoj liniji MDBK. Pojava citopatogenog efekta na kulturi ćelija, nakon 48h, odnosno 72h, utvrđena je kod osam uzoraka nosnih briseva (8/119; 6.7%). Identifikacija izolovanih sojeva BPIV3 je izvršena primenom virus neutralizacionog testa. Dodatno, svi uzorci nosnih briseva goveda su ispitani na prisustvo BPIV3 i primenom metode RT-PCR uz korišćenje prajmera specifičnih za deo HN gena virusa. Prisustvo virusne nukleinske kiseline je utvrđeno kod osam uzoraka nosnih briseva (8/119; 6.7%), koji su bili pozitivni i na izolaciji virusa. Molekularna karakterizacija zasnovana na sekvenciranju dela HN gena izolata BPIV3 iz Srbije je potvrdila da svi pripadaju genotipu C BPIV3 (BPIV3c). Oni su se u filogenetskom stablu granali u tri različite grane koje su međusobno veoma slične (98.1% do 99.8%). Izolati BPIV3 iz Srbije su pokazali visok stepen sličnosti nukleotidnih sekvenci sa BPIV3c sojevima SD0805, SD0809 i SD0835 iz Kine (97.92% do 99.7%), odnosno sa BPIV3c sojevima 12Q061 iz Južne Koreje, HS9 iz Japana i TVMDL16 i TVMDL20 iz Amerike (sličnost od 97.1% do 98.8%), kao i različitosti u odnosu na nukleotidne sekvence sojeva TVMDL15 i TVMDL17 izolovanih u Americi i soja Q5592 izolovanog u Australiji, a koji su pripadali genotipu B BPIV3 (sličnost od 79.9% do 82.3%). Slična razlika je utvrđena i sa nukleotidnim sekvencama sojeva virusa, poreklom iz različitih država, svrstanih u genotip A BPIV3, a objavljenih u genskoj bazi podataka (NCBI GenBank).
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia
T1  - Izolacija i molekularna detekcija parainfluenca 3 virusa kod goveda u Srbiji
VL  - 66
IS  - 4
SP  - 509
EP  - 519
DO  - 10.1515/acve-2016-0044
ER  - 

@article{
author = "Veljović, Ljubiša and Knežević, Aleksandra and Milić, Nenad and Krnjaić, Dejan and Miković, Radoš and Zorić, Andrea and Marković, Maja and Milićević, Vesna and Stamenković, Miodrag and Stanojević, Maja and Maksimović-Zorić, Jelena and Petrović, Tamaš and Nišavić, Jakov",
year = "2016",
abstract = "The presence of bovine parainfluenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight samples (8/119; 6.7%). The confirmation of isolated strains of BPIV3 was done by the virus-neutralization test. In addition, all samples of bovine nasal swabs were also examined for the presence of BPIV3 virus using RT-PCR with primers specific for the part of HN gene. The presence of BPIV3 was detected in eight samples (8/119; 6.7%) that were also positive upon virus isolation. The molecular characterization based on nucleotide sequencing of the part of the HN gene showed that all BPIV3 isolates belonged to genotype C of BPIV3. They branched in one distinct cluster with three different branches, but these branches were very similar to each other (98.1% to 99.8%). Serbian BPIV3c isolates were most similar to the Chinese BPIV3c isolates SD0805, SD0809 and SD0835 (from 97.92% to 99.7%), and to South Korean (12Q061), Japanese (HS9) and American (TVMDL16 and TVMDL20) BPIV3c strains (from 97.1% to 98.8%), and distinct from American (TVMDL15and TVMDL17) and Australian (Q5592) BPI3V genotype B strains (only 79.9% to 82.3% similarity), as well as from the genotype A BPIV3 strains from different countries published in GenBank., Ukupno je ispitano 119 uzoraka nosnih briseva goveda na prisustvo parainfluenca 3 virusa goveda (bovine parainfluenza virus type, eng. - 3 BPIV3). Iz svih uzoraka nosnih briseva je vršena izolacija virusa na ćelijskoj liniji MDBK. Pojava citopatogenog efekta na kulturi ćelija, nakon 48h, odnosno 72h, utvrđena je kod osam uzoraka nosnih briseva (8/119; 6.7%). Identifikacija izolovanih sojeva BPIV3 je izvršena primenom virus neutralizacionog testa. Dodatno, svi uzorci nosnih briseva goveda su ispitani na prisustvo BPIV3 i primenom metode RT-PCR uz korišćenje prajmera specifičnih za deo HN gena virusa. Prisustvo virusne nukleinske kiseline je utvrđeno kod osam uzoraka nosnih briseva (8/119; 6.7%), koji su bili pozitivni i na izolaciji virusa. Molekularna karakterizacija zasnovana na sekvenciranju dela HN gena izolata BPIV3 iz Srbije je potvrdila da svi pripadaju genotipu C BPIV3 (BPIV3c). Oni su se u filogenetskom stablu granali u tri različite grane koje su međusobno veoma slične (98.1% do 99.8%). Izolati BPIV3 iz Srbije su pokazali visok stepen sličnosti nukleotidnih sekvenci sa BPIV3c sojevima SD0805, SD0809 i SD0835 iz Kine (97.92% do 99.7%), odnosno sa BPIV3c sojevima 12Q061 iz Južne Koreje, HS9 iz Japana i TVMDL16 i TVMDL20 iz Amerike (sličnost od 97.1% do 98.8%), kao i različitosti u odnosu na nukleotidne sekvence sojeva TVMDL15 i TVMDL17 izolovanih u Americi i soja Q5592 izolovanog u Australiji, a koji su pripadali genotipu B BPIV3 (sličnost od 79.9% do 82.3%). Slična razlika je utvrđena i sa nukleotidnim sekvencama sojeva virusa, poreklom iz različitih država, svrstanih u genotip A BPIV3, a objavljenih u genskoj bazi podataka (NCBI GenBank).",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia, Izolacija i molekularna detekcija parainfluenca 3 virusa kod goveda u Srbiji",
volume = "66",
number = "4",
pages = "509-519",
doi = "10.1515/acve-2016-0044"
}

Veljović, L., Knežević, A., Milić, N., Krnjaić, D., Miković, R., Zorić, A., Marković, M., Milićević, V., Stamenković, M., Stanojević, M., Maksimović-Zorić, J., Petrović, T.,& Nišavić, J.. (2016). Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(4), 509-519.
https://doi.org/10.1515/acve-2016-0044

Veljović L, Knežević A, Milić N, Krnjaić D, Miković R, Zorić A, Marković M, Milićević V, Stamenković M, Stanojević M, Maksimović-Zorić J, Petrović T, Nišavić J. Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia. in Acta Veterinaria-Beograd. 2016;66(4):509-519.
doi:10.1515/acve-2016-0044 .

Veljović, Ljubiša, Knežević, Aleksandra, Milić, Nenad, Krnjaić, Dejan, Miković, Radoš, Zorić, Andrea, Marković, Maja, Milićević, Vesna, Stamenković, Miodrag, Stanojević, Maja, Maksimović-Zorić, Jelena, Petrović, Tamaš, Nišavić, Jakov, "Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia" in Acta Veterinaria-Beograd, 66, no. 4 (2016):509-519,
https://doi.org/10.1515/acve-2016-0044 . .

TY  - JOUR
AU  - Nišavić, Jakov
AU  - Zorić, Andrea
AU  - Milić, Nenad
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1392
AB  - Porcine circovirus 2 (PCV2) belongs to the family Circoviridae, genus Circovirus. Infection of swine caused by this virus is manifested in several different clinical forms, leading to significant economic losses in swine production worldwide. For this reason, prompt and precise diagnostics of this swine infection is of great importance. For this purpose today there are used molecular methods of virological diagnostics such as polymerase chain reaction (PCR), and real-time method PCR, that is direct sequencing method by Sanger.
AB  - Svinjski cirkovirus 2 (PCV2) pripada familiji Circoviridae i rodu Circovirus. Infekcija svinja izazvana ovim virusom se ispoljava u više različitih kliničkih formi dovodeći do značajnih ekonomskih gubitaka u proizvodnji svinja širom sveta. Iz navedenog razloga brza i precizna dijagnostika ove infekcije svinja je od izuzetnog značaja. U te svrhe se danas koriste molekularne metode virusološke dijagnostike kao što su lančana reakcija polimeraze (PCR), zatim metoda real-time PCR, odnosno metoda dierktnog sekvenciranja po Sanger-u.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - The application of molecular methods in the diagnostics of infection of swine caused by porcine Circovirus 2
T1  - Ispol'zovanie molekuljarnyh metodov v diagnostike infekcii svinej, vyzvannoj svinym Cirkovirusom 2 tipa
T1  - Primena molekularnih metoda u dijagnostici infekcije svinja izazvane svinjskim Cirkovirusom 2
VL  - 70
IS  - 5-6
SP  - 249
EP  - 258
DO  - 10.2298/VETGL1606249N
ER  - 

@article{
author = "Nišavić, Jakov and Zorić, Andrea and Milić, Nenad",
year = "2016",
abstract = "Porcine circovirus 2 (PCV2) belongs to the family Circoviridae, genus Circovirus. Infection of swine caused by this virus is manifested in several different clinical forms, leading to significant economic losses in swine production worldwide. For this reason, prompt and precise diagnostics of this swine infection is of great importance. For this purpose today there are used molecular methods of virological diagnostics such as polymerase chain reaction (PCR), and real-time method PCR, that is direct sequencing method by Sanger., Svinjski cirkovirus 2 (PCV2) pripada familiji Circoviridae i rodu Circovirus. Infekcija svinja izazvana ovim virusom se ispoljava u više različitih kliničkih formi dovodeći do značajnih ekonomskih gubitaka u proizvodnji svinja širom sveta. Iz navedenog razloga brza i precizna dijagnostika ove infekcije svinja je od izuzetnog značaja. U te svrhe se danas koriste molekularne metode virusološke dijagnostike kao što su lančana reakcija polimeraze (PCR), zatim metoda real-time PCR, odnosno metoda dierktnog sekvenciranja po Sanger-u.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "The application of molecular methods in the diagnostics of infection of swine caused by porcine Circovirus 2, Ispol'zovanie molekuljarnyh metodov v diagnostike infekcii svinej, vyzvannoj svinym Cirkovirusom 2 tipa, Primena molekularnih metoda u dijagnostici infekcije svinja izazvane svinjskim Cirkovirusom 2",
volume = "70",
number = "5-6",
pages = "249-258",
doi = "10.2298/VETGL1606249N"
}

Nišavić, J., Zorić, A.,& Milić, N.. (2016). The application of molecular methods in the diagnostics of infection of swine caused by porcine Circovirus 2. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(5-6), 249-258.
https://doi.org/10.2298/VETGL1606249N

Nišavić J, Zorić A, Milić N. The application of molecular methods in the diagnostics of infection of swine caused by porcine Circovirus 2. in Veterinarski Glasnik. 2016;70(5-6):249-258.
doi:10.2298/VETGL1606249N .

Nišavić, Jakov, Zorić, Andrea, Milić, Nenad, "The application of molecular methods in the diagnostics of infection of swine caused by porcine Circovirus 2" in Veterinarski Glasnik, 70, no. 5-6 (2016):249-258,
https://doi.org/10.2298/VETGL1606249N . .

TY  - JOUR
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Borozan, Sunčica
AU  - Zorić, Andrea
AU  - Lazić, Sava
AU  - Petrović, Tamaš
AU  - Rašić, Zoran
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1221
AB  - The objective of our work was to investigate some biological characteristics of purified glycoprotein subunits of Newcastle disease virus strain PHY-LMV.42 isolated from pigeons for the purpose of vaccine production. PHY-LMV.42 strain of Newcastle disease virus was multiplied by successive passages in embryonated eggs and identified by the methods of Reverse transcriptase PCR and Real-Time PCR along with F gene sequencing. Proving the presence of HN and F antigene in the virus subunits samples was carried out by hemagglutination inhibition method with referent immune sera. Biochemical characterization of glycoprotein subunits was performed by SDS-PAGE method as well as liquid chromatography with mass spectrometry (LC ESI-TOF-MS/MS). Testing for the virus subunits immunogenicity was carried out in biological experiment on 75 laying hens Tetra-SSL and 25 chickens Isa Brown by inducing an artificial infection with Hertz 33 strain of the virus. Low concentrations of the virus antigens of 0.36 mg/ml along with glycoprotein fractions of 77 i 58 kDa manifested a strong hemagglutination activity of 4096 HJ/0,1ml. The subunit vaccines of 256 and 128 HJ/0.5 ml induced a protective immune response in all the vaccinated animals. Based on the obtained results it can be concluded that low concentrations of purified virus subunits of PHY-LMV.42 strain can be used for preparing of effective vaccines.
AB  - Cilj našeg istraživanja je bilo ispitivanje bioloških karakteristika prečišćenih glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti izolovanog iz golubova radi njihovog korišćenja za pripremanje vakcine. Soj PHY-LMV.42 virusa Newcastle bolesti je umnožavan sukcesivnim pasažama u kokošijim embrionima i identifikovan metodama Reverse transcriptase PCR i Real- Time PCR uz sekvenciranje F gena. Dokazivanje prisustva HN i F antigena u uzorcima virusnih subjedinica vršeno je metodom inhibicije hemaglutinacije sa referentnim imunim serumima. Biohemijska karakterizacija glikoproteinskih subjedinica izvršena je primenom metoda SDS-PAGE i tečne hromatografije sa masenom spektrometrijom (LC ESI-TOF-MS/MS). Ispitivanje imunogenosti virusnih subjedinica sprovedeno je u biološkom ogledu na ukupno 75 kokoši nosilja Tetra-SSL i 25 pilića Isa Brown uz izvođenje veštačke infekcije sojem Hertz 33 navedenog virusa. Niske koncentracije virusnih antigena od 0,36 mg/ml sa glikoproteinskim frakcijama od 77 i 58 kDa su ispoljavale snažnu hemaglutinacionu aktivnost od 4096 HJ/0,1ml. Subjedinične vakcine od 256 i 128 HJ/0,5 ml, indukovale su imunološki odgovor zaštitnog karaktera kod svih vakcinisanih životinja. Na osnovu dobijenih rezultata može se zaključiti da se niske koncentracije prečišćenih virusnih subjedinica soja PHY-LMV.42 mogu koristiti za pripremanje efikasne vakcine. PR Projekat Ministarstva nauke Republike Srbije, br. TR 31008.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus
T1  - Ispitivanje nekih bioloških karakteristika glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti živine
VL  - 69
IS  - 5-6
SP  - 337
EP  - 355
DO  - 10.2298/VETGL1506337M
ER  - 

@article{
author = "Milić, Nenad and Nišavić, Jakov and Borozan, Sunčica and Zorić, Andrea and Lazić, Sava and Petrović, Tamaš and Rašić, Zoran",
year = "2015",
abstract = "The objective of our work was to investigate some biological characteristics of purified glycoprotein subunits of Newcastle disease virus strain PHY-LMV.42 isolated from pigeons for the purpose of vaccine production. PHY-LMV.42 strain of Newcastle disease virus was multiplied by successive passages in embryonated eggs and identified by the methods of Reverse transcriptase PCR and Real-Time PCR along with F gene sequencing. Proving the presence of HN and F antigene in the virus subunits samples was carried out by hemagglutination inhibition method with referent immune sera. Biochemical characterization of glycoprotein subunits was performed by SDS-PAGE method as well as liquid chromatography with mass spectrometry (LC ESI-TOF-MS/MS). Testing for the virus subunits immunogenicity was carried out in biological experiment on 75 laying hens Tetra-SSL and 25 chickens Isa Brown by inducing an artificial infection with Hertz 33 strain of the virus. Low concentrations of the virus antigens of 0.36 mg/ml along with glycoprotein fractions of 77 i 58 kDa manifested a strong hemagglutination activity of 4096 HJ/0,1ml. The subunit vaccines of 256 and 128 HJ/0.5 ml induced a protective immune response in all the vaccinated animals. Based on the obtained results it can be concluded that low concentrations of purified virus subunits of PHY-LMV.42 strain can be used for preparing of effective vaccines., Cilj našeg istraživanja je bilo ispitivanje bioloških karakteristika prečišćenih glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti izolovanog iz golubova radi njihovog korišćenja za pripremanje vakcine. Soj PHY-LMV.42 virusa Newcastle bolesti je umnožavan sukcesivnim pasažama u kokošijim embrionima i identifikovan metodama Reverse transcriptase PCR i Real- Time PCR uz sekvenciranje F gena. Dokazivanje prisustva HN i F antigena u uzorcima virusnih subjedinica vršeno je metodom inhibicije hemaglutinacije sa referentnim imunim serumima. Biohemijska karakterizacija glikoproteinskih subjedinica izvršena je primenom metoda SDS-PAGE i tečne hromatografije sa masenom spektrometrijom (LC ESI-TOF-MS/MS). Ispitivanje imunogenosti virusnih subjedinica sprovedeno je u biološkom ogledu na ukupno 75 kokoši nosilja Tetra-SSL i 25 pilića Isa Brown uz izvođenje veštačke infekcije sojem Hertz 33 navedenog virusa. Niske koncentracije virusnih antigena od 0,36 mg/ml sa glikoproteinskim frakcijama od 77 i 58 kDa su ispoljavale snažnu hemaglutinacionu aktivnost od 4096 HJ/0,1ml. Subjedinične vakcine od 256 i 128 HJ/0,5 ml, indukovale su imunološki odgovor zaštitnog karaktera kod svih vakcinisanih životinja. Na osnovu dobijenih rezultata može se zaključiti da se niske koncentracije prečišćenih virusnih subjedinica soja PHY-LMV.42 mogu koristiti za pripremanje efikasne vakcine. PR Projekat Ministarstva nauke Republike Srbije, br. TR 31008.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus, Ispitivanje nekih bioloških karakteristika glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti živine",
volume = "69",
number = "5-6",
pages = "337-355",
doi = "10.2298/VETGL1506337M"
}

Milić, N., Nišavić, J., Borozan, S., Zorić, A., Lazić, S., Petrović, T.,& Rašić, Z.. (2015). Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(5-6), 337-355.
https://doi.org/10.2298/VETGL1506337M

Milić N, Nišavić J, Borozan S, Zorić A, Lazić S, Petrović T, Rašić Z. Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus. in Veterinarski Glasnik. 2015;69(5-6):337-355.
doi:10.2298/VETGL1506337M .

Milić, Nenad, Nišavić, Jakov, Borozan, Sunčica, Zorić, Andrea, Lazić, Sava, Petrović, Tamaš, Rašić, Zoran, "Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus" in Veterinarski Glasnik, 69, no. 5-6 (2015):337-355,
https://doi.org/10.2298/VETGL1506337M . .

TY  - JOUR
AU  - Veljović, Ljubiša
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Nišavić, Jakov
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1092
AB  - Bovine parainfluenza 3 virus (PI3) causes respiratory infections in cattle and sheep with great economic losses in livestock. The aim of this investigation was to determine the significance of molecular methods in the identification of isolated strains of PI3 virus. Twenty cattle nasal swabs were analyzed for the presence of PI3 using the standard virology method of virus isolation in MBDK cell line and virus neutralization test. The identification of isolated strains was confirmed by RT-PCR and method of direct sequencing with primers for PI3 fusion (F) protein gene. PI3 virus was isolated and identified in four nasal swabs using the standard virology method and RT-PCR. The analysis of nucleotide sequences of isolated PI3 strains showed high similarity with sequences isolated from cattle in Asia. Our results showed that molecular methods are very useful in the diagnosis of PI3 infections as well as for the identification and characterization of PI3 strains in Serbia.
PB  - Srpsko biološko društvo, Beograd
T2  - Archives of Biological Sciences
T1  - The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle
VL  - 66
IS  - 2
SP  - 491
EP  - 496
DO  - 10.2298/ABS1402491V
ER  - 

@article{
author = "Veljović, Ljubiša and Knežević, Aleksandra and Milić, Nenad and Nišavić, Jakov",
year = "2014",
abstract = "Bovine parainfluenza 3 virus (PI3) causes respiratory infections in cattle and sheep with great economic losses in livestock. The aim of this investigation was to determine the significance of molecular methods in the identification of isolated strains of PI3 virus. Twenty cattle nasal swabs were analyzed for the presence of PI3 using the standard virology method of virus isolation in MBDK cell line and virus neutralization test. The identification of isolated strains was confirmed by RT-PCR and method of direct sequencing with primers for PI3 fusion (F) protein gene. PI3 virus was isolated and identified in four nasal swabs using the standard virology method and RT-PCR. The analysis of nucleotide sequences of isolated PI3 strains showed high similarity with sequences isolated from cattle in Asia. Our results showed that molecular methods are very useful in the diagnosis of PI3 infections as well as for the identification and characterization of PI3 strains in Serbia.",
publisher = "Srpsko biološko društvo, Beograd",
journal = "Archives of Biological Sciences",
title = "The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle",
volume = "66",
number = "2",
pages = "491-496",
doi = "10.2298/ABS1402491V"
}

Veljović, L., Knežević, A., Milić, N.,& Nišavić, J.. (2014). The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd., 66(2), 491-496.
https://doi.org/10.2298/ABS1402491V

Veljović L, Knežević A, Milić N, Nišavić J. The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle. in Archives of Biological Sciences. 2014;66(2):491-496.
doi:10.2298/ABS1402491V .

Veljović, Ljubiša, Knežević, Aleksandra, Milić, Nenad, Nišavić, Jakov, "The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle" in Archives of Biological Sciences, 66, no. 2 (2014):491-496,
https://doi.org/10.2298/ABS1402491V . .

TY  - JOUR
AU  - Milić, Nenad
AU  - Lazić, S.
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Nišavić, Jakov
AU  - Resanović, Radmila
AU  - Petrović, Tamaš
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/864
AB  - Five strains of Newcastle disease virus (NDV) were obtained from poultry in Vojvodina, Serbia during the outbreaks in 2006 and 2007. These isolates were confirmed and genotypically characterized by reverse transcription polymerase chain reaction (RT-PCR) with primer specific to the viral fusion (F) protein (572bp), and by sequencing of partial F gene for phylogenetic analysis. Phylogenetic analysis showed that all five isolated strains of Newcastle disease virus belong to genotype VII. At the same time, all five isolates were clustered in NDV subtype VIId. The examined NDV isolates express high similarity to each other (99.7-100%) and group together with the strains of Newcastle disease virus isolated previously from wild birds in Serbia during the same 2006 - 2007 outbreak. The analysis of the isolates F gene cleavage sites has shown that all five isolated strains of Newcastle disease virus had a cleavage site motif 112R-R-Q-K-R-F-117 characteristic for highly virulent, velogenic strains.
AB  - Pet sojeva virusa Newcastle bolesti (NDV) je izolovano iz uzoraka suspektnog materijala poreklom od živine 2006. i 2007.godine tokom epizootije atipične kuge živine na teritoriji Vojvodine, Srbija. Ovi izolati su potvrđeni i genotipski tipizirani primenom metoda RT-PCR uz korišćenje prajmera specifičnih za deo genoma virusa koji kodira sintezu fuzionog F proteina (572bp) i sekvenciranjem dela F gena sa filogenetskom analizom. Filogenetska analiza je ukazala da je svih pet izolovanih sojeva virusa Newcastle bolesti pripadalo genotipu VII. Istovremeno, svih pet sojeva je grupisano u podtip VIId navedenog virusa. Izolovani sojevi virusa Newcastle bolesti su međusobno bili veoma slični (99,7-100%) i grupisali su se sa sojevima virusa prethodno izolovanih iz divljih ptica u Srbiji tokom izbijanja bolesti 2006. i 2007. godine. Molekularnom karakterizacijom gena na mestu deobe fuzionog F proteina ustanovljeno je da svih pet izolovanih sojeva virusa pripada visoko virulentnim velogenim sojevima.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Molecular characterization of some strains of Newcastle disease virus isolated in Province of Vojvodina, Republic of Serbia
T1  - Molekularna karakterizacija nekih sojeva virusa Newcastle bolesti izolovanih u Pokrajini Vojvodini Republike Srbije
VL  - 62
IS  - 4
SP  - 365
EP  - 374
DO  - 10.2298/AVB1204365M
ER  - 

@article{
author = "Milić, Nenad and Lazić, S. and Vidanović, Dejan and Šekler, Milanko and Nišavić, Jakov and Resanović, Radmila and Petrović, Tamaš",
year = "2012",
abstract = "Five strains of Newcastle disease virus (NDV) were obtained from poultry in Vojvodina, Serbia during the outbreaks in 2006 and 2007. These isolates were confirmed and genotypically characterized by reverse transcription polymerase chain reaction (RT-PCR) with primer specific to the viral fusion (F) protein (572bp), and by sequencing of partial F gene for phylogenetic analysis. Phylogenetic analysis showed that all five isolated strains of Newcastle disease virus belong to genotype VII. At the same time, all five isolates were clustered in NDV subtype VIId. The examined NDV isolates express high similarity to each other (99.7-100%) and group together with the strains of Newcastle disease virus isolated previously from wild birds in Serbia during the same 2006 - 2007 outbreak. The analysis of the isolates F gene cleavage sites has shown that all five isolated strains of Newcastle disease virus had a cleavage site motif 112R-R-Q-K-R-F-117 characteristic for highly virulent, velogenic strains., Pet sojeva virusa Newcastle bolesti (NDV) je izolovano iz uzoraka suspektnog materijala poreklom od živine 2006. i 2007.godine tokom epizootije atipične kuge živine na teritoriji Vojvodine, Srbija. Ovi izolati su potvrđeni i genotipski tipizirani primenom metoda RT-PCR uz korišćenje prajmera specifičnih za deo genoma virusa koji kodira sintezu fuzionog F proteina (572bp) i sekvenciranjem dela F gena sa filogenetskom analizom. Filogenetska analiza je ukazala da je svih pet izolovanih sojeva virusa Newcastle bolesti pripadalo genotipu VII. Istovremeno, svih pet sojeva je grupisano u podtip VIId navedenog virusa. Izolovani sojevi virusa Newcastle bolesti su međusobno bili veoma slični (99,7-100%) i grupisali su se sa sojevima virusa prethodno izolovanih iz divljih ptica u Srbiji tokom izbijanja bolesti 2006. i 2007. godine. Molekularnom karakterizacijom gena na mestu deobe fuzionog F proteina ustanovljeno je da svih pet izolovanih sojeva virusa pripada visoko virulentnim velogenim sojevima.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Molecular characterization of some strains of Newcastle disease virus isolated in Province of Vojvodina, Republic of Serbia, Molekularna karakterizacija nekih sojeva virusa Newcastle bolesti izolovanih u Pokrajini Vojvodini Republike Srbije",
volume = "62",
number = "4",
pages = "365-374",
doi = "10.2298/AVB1204365M"
}

Milić, N., Lazić, S., Vidanović, D., Šekler, M., Nišavić, J., Resanović, R.,& Petrović, T.. (2012). Molecular characterization of some strains of Newcastle disease virus isolated in Province of Vojvodina, Republic of Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(4), 365-374.
https://doi.org/10.2298/AVB1204365M

Milić N, Lazić S, Vidanović D, Šekler M, Nišavić J, Resanović R, Petrović T. Molecular characterization of some strains of Newcastle disease virus isolated in Province of Vojvodina, Republic of Serbia. in Acta Veterinaria-Beograd. 2012;62(4):365-374.
doi:10.2298/AVB1204365M .

Milić, Nenad, Lazić, S., Vidanović, Dejan, Šekler, Milanko, Nišavić, Jakov, Resanović, Radmila, Petrović, Tamaš, "Molecular characterization of some strains of Newcastle disease virus isolated in Province of Vojvodina, Republic of Serbia" in Acta Veterinaria-Beograd, 62, no. 4 (2012):365-374,
https://doi.org/10.2298/AVB1204365M . .

TY  - JOUR
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Polaček, Vladimir
AU  - Vasković, Nikola
AU  - Ašanin, Ružica
AU  - Milić, Nenad
AU  - Nišavić, Jakov
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/945
AB  - Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007. Newcastle disease virus strains were isolated from four samples. The identification of isolated strains was done by using the hemagglutination and hemagglutination-inhibition tests. The nucleic acid of the ND virus was identified in all the four samples It was confirmed that all the isolated strains were velogenic strains. Analysis of the nucleotide sequences of the gene encoding the F cleavage site of the fusion F protein showed the presence of motifs 112RRQKRFIG119, characteristic for the velogenic strains of the ND virus. Phylogenetic analysis of the F gene sequences revealed that all isolated strains of the virus belong to class II and genotype VIId.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Application of standard and molecular methods for the diagnosis of Newcastle disease
VL  - 64
IS  - 4
SP  - 1433
EP  - 1437
DO  - 10.2298/ABS1204433V
ER  - 

@article{
author = "Vidanović, Dejan and Šekler, Milanko and Polaček, Vladimir and Vasković, Nikola and Ašanin, Ružica and Milić, Nenad and Nišavić, Jakov",
year = "2012",
abstract = "Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007. Newcastle disease virus strains were isolated from four samples. The identification of isolated strains was done by using the hemagglutination and hemagglutination-inhibition tests. The nucleic acid of the ND virus was identified in all the four samples It was confirmed that all the isolated strains were velogenic strains. Analysis of the nucleotide sequences of the gene encoding the F cleavage site of the fusion F protein showed the presence of motifs 112RRQKRFIG119, characteristic for the velogenic strains of the ND virus. Phylogenetic analysis of the F gene sequences revealed that all isolated strains of the virus belong to class II and genotype VIId.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Application of standard and molecular methods for the diagnosis of Newcastle disease",
volume = "64",
number = "4",
pages = "1433-1437",
doi = "10.2298/ABS1204433V"
}

Vidanović, D., Šekler, M., Polaček, V., Vasković, N., Ašanin, R., Milić, N.,& Nišavić, J.. (2012). Application of standard and molecular methods for the diagnosis of Newcastle disease. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(4), 1433-1437.
https://doi.org/10.2298/ABS1204433V

Vidanović D, Šekler M, Polaček V, Vasković N, Ašanin R, Milić N, Nišavić J. Application of standard and molecular methods for the diagnosis of Newcastle disease. in Archives of Biological Sciences. 2012;64(4):1433-1437.
doi:10.2298/ABS1204433V .

Vidanović, Dejan, Šekler, Milanko, Polaček, Vladimir, Vasković, Nikola, Ašanin, Ružica, Milić, Nenad, Nišavić, Jakov, "Application of standard and molecular methods for the diagnosis of Newcastle disease" in Archives of Biological Sciences, 64, no. 4 (2012):1433-1437,
https://doi.org/10.2298/ABS1204433V . .