TY  - JOUR
AU  - Dimitrijević, Blagoje
AU  - Borozan, Sunčica
AU  - Katić-Radivojević, Sofija
AU  - Stojanović, Srđan
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/855
AB  - The objective of this study was to estimate and evaluate oxidative/nitrosative stress parameters in sheep infected with Strongyloides papillosus and after antihelminthic treatment with albendazole (ABZ). This parasite, especially during development stages can seriously damage parenchaematous organs during migration within the host. The presence of parasites leads to increased productions of reactive oxygen species (ROS) and reactive nitrogen species (RNS). It is also well known that certain drugs can be very harmful for the delicate oxidant-antioxidant equilibrium, provoking oxidative stress during their biotransformation. ABZ is a broad spectrum antihelminthic drug, frequently used in veterinary medicine for therapy of parasitic infections. The current research was performed on female Wurttemberg sheep (n = 48). The distribution of parasites in sheep was evaluated using the native smear coprological technique, by sedimentation and flotation methods, revealing the presence of S. papillosus. The degree of infection intensity per sheep was quantitatively established by the method of McMaster, the animals having been divided into three groups according to the intensity of infection; mild, moderate and high. The control group consisted of sheep negative to the parasites. After determining the type of parasite infection, the sheep were treated with ABZ, per orally, in single doses of 5 mg/kg per body weight. Sampling of feces for parasitological and blood for biochemical assaying was performed on the 0 and 21st day after treatment with ABZ. The oxidative stress parameters were measured for catalase activity (CAT), the red cell membrane damage by level of malondialdehyde (MDA), while carbonyl and thiol plasma protein group concentrations were used as indicators of the degree of protein oxidative modification. The activity of Cu,Zn-superoxide dismutase (SOD) and relative distribution of lactate dehydrogenase (LDH1-LDH5) activity were determined electrophoretically. The distribution of LDH isoenzymes in sheep moderately and highly infected with S. papillosus revealed that the parasite induced damage to the myocardial (LDH2), lung (LDH3) and liver cells (LDH5) in infected animals, while ABZ treatment only damaged liver cells (LDH5). The MDA concentration revealed that lipid peroxidation increased both in the presence of parasites and the antihelminthic formulation tested (p < 0.001) when compared to the control sheep, while the increase of carbonyl concentration (p < 0.001), as well as the observed decrease of thiol concentration (p < 0.001) indicated significant oxidative damage of plasma proteins in experimental sheep, when compared to the control animals. Our results indicate that S. papillosus induces oxidative/nitrosative stress in sheep. The antihelminthic treatment with ABZ further promotes the disbalance of oxidative-antioxidative equilibrium in all tested sheep.
PB  - Elsevier, Amsterdam
T2  - Veterinary Parasitology
T1  - Effects of infection intensity with Strongyloides papillosus and albendazole treatment on development of oxidative/nitrosative stress in sheep
VL  - 186
IS  - 3-4
SP  - 364
EP  - 375
DO  - 10.1016/j.vetpar.2011.11.017
ER  - 

@article{
author = "Dimitrijević, Blagoje and Borozan, Sunčica and Katić-Radivojević, Sofija and Stojanović, Srđan",
year = "2012",
abstract = "The objective of this study was to estimate and evaluate oxidative/nitrosative stress parameters in sheep infected with Strongyloides papillosus and after antihelminthic treatment with albendazole (ABZ). This parasite, especially during development stages can seriously damage parenchaematous organs during migration within the host. The presence of parasites leads to increased productions of reactive oxygen species (ROS) and reactive nitrogen species (RNS). It is also well known that certain drugs can be very harmful for the delicate oxidant-antioxidant equilibrium, provoking oxidative stress during their biotransformation. ABZ is a broad spectrum antihelminthic drug, frequently used in veterinary medicine for therapy of parasitic infections. The current research was performed on female Wurttemberg sheep (n = 48). The distribution of parasites in sheep was evaluated using the native smear coprological technique, by sedimentation and flotation methods, revealing the presence of S. papillosus. The degree of infection intensity per sheep was quantitatively established by the method of McMaster, the animals having been divided into three groups according to the intensity of infection; mild, moderate and high. The control group consisted of sheep negative to the parasites. After determining the type of parasite infection, the sheep were treated with ABZ, per orally, in single doses of 5 mg/kg per body weight. Sampling of feces for parasitological and blood for biochemical assaying was performed on the 0 and 21st day after treatment with ABZ. The oxidative stress parameters were measured for catalase activity (CAT), the red cell membrane damage by level of malondialdehyde (MDA), while carbonyl and thiol plasma protein group concentrations were used as indicators of the degree of protein oxidative modification. The activity of Cu,Zn-superoxide dismutase (SOD) and relative distribution of lactate dehydrogenase (LDH1-LDH5) activity were determined electrophoretically. The distribution of LDH isoenzymes in sheep moderately and highly infected with S. papillosus revealed that the parasite induced damage to the myocardial (LDH2), lung (LDH3) and liver cells (LDH5) in infected animals, while ABZ treatment only damaged liver cells (LDH5). The MDA concentration revealed that lipid peroxidation increased both in the presence of parasites and the antihelminthic formulation tested (p < 0.001) when compared to the control sheep, while the increase of carbonyl concentration (p < 0.001), as well as the observed decrease of thiol concentration (p < 0.001) indicated significant oxidative damage of plasma proteins in experimental sheep, when compared to the control animals. Our results indicate that S. papillosus induces oxidative/nitrosative stress in sheep. The antihelminthic treatment with ABZ further promotes the disbalance of oxidative-antioxidative equilibrium in all tested sheep.",
publisher = "Elsevier, Amsterdam",
journal = "Veterinary Parasitology",
title = "Effects of infection intensity with Strongyloides papillosus and albendazole treatment on development of oxidative/nitrosative stress in sheep",
volume = "186",
number = "3-4",
pages = "364-375",
doi = "10.1016/j.vetpar.2011.11.017"
}

Dimitrijević, B., Borozan, S., Katić-Radivojević, S.,& Stojanović, S.. (2012). Effects of infection intensity with Strongyloides papillosus and albendazole treatment on development of oxidative/nitrosative stress in sheep. in Veterinary Parasitology
Elsevier, Amsterdam., 186(3-4), 364-375.
https://doi.org/10.1016/j.vetpar.2011.11.017

Dimitrijević B, Borozan S, Katić-Radivojević S, Stojanović S. Effects of infection intensity with Strongyloides papillosus and albendazole treatment on development of oxidative/nitrosative stress in sheep. in Veterinary Parasitology. 2012;186(3-4):364-375.
doi:10.1016/j.vetpar.2011.11.017 .

Dimitrijević, Blagoje, Borozan, Sunčica, Katić-Radivojević, Sofija, Stojanović, Srđan, "Effects of infection intensity with Strongyloides papillosus and albendazole treatment on development of oxidative/nitrosative stress in sheep" in Veterinary Parasitology, 186, no. 3-4 (2012):364-375,
https://doi.org/10.1016/j.vetpar.2011.11.017 . .

TY  - JOUR
AU  - Đurđević, S.
AU  - Jezdimirović, Milanka
AU  - Aleksić, Nevenka
AU  - Branković, Nataša
AU  - Pržulj, D.
AU  - Cicović, B.
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/684
AB  - Concentrations of the NSAID metamizol sodium (MmNa) in the synovial fluid and hyaline cartilage of inflamed knee and elbow joints of pigs after i.v. application and iontophoresis (IPh) were investigated. The research was conducted on 14 male pigs divided equally into two experimental groups and exposed to artificial inflammation of knees and elbows prior to the application of MmNa. The first group (A) was administered 2.5 g (twice the maximum therapeutic dose) MmNa intravenously, whilst the second group (B) was exposed to the same dose, but applied by IPh into contralateral knee and elbow joints. Four hours after the application of MmNa biopsies of the affected knee and elbow joints were performed. The average concentration of MmNa in the synovial fluid of inflamed joints in group A was 9.81±1.96μg/g, while in group B was 170.66±2.07 μg/g, being 17 times higher. The average concentration of MmNa in the hyaline cartilage was 2.29±1.16μg/g following i.v. administration and 98.36±21.58μg/g after IPh, i.e. approximatelly 43 times higher. This led to the conclusion that IPh of MmNa, resulting in incomparably higher concentrations in inflamed joints without any adverse systemic effects, has an advantage over i.v. application.
AB  - U sinovijalnoj tečnosti i hijalinoj hrskavici inflamiranog kolenog i lakatnog zgloba svinja određivane su koncentracije metamizol-natrijuma (MmNa) aplikovanog i.v. i putem elektroforeze. Arteficijelno zapaljenje kolena i lakta prasadi izazvano je terpentinskim uljem. Metamizol-natrijum je primenjen u dozi od 2.5 g u prvoj grupi prasadi intravenski, a u drugoj putem elektroforeze u kontalateralni koleni i lakatni zglob. Četiri sata posle aplikacije MmNa urađena je biopsija tretiranih zglobova. Prosečna koncentracija MmNa u sinovijalnoj tečnosti inflamiranih zglobova prasadi posle i.v. aplikacije iznosila je 9,81±1,96μg/g, a posle elektroforeze bila je 170,66±2,07μg/g tkiva, odnosno oko 17 puta viša. Izmerena prosečna koncentracija MmNa u hijalinoj hrskavici iznosila je 2,29±1,16μg/g posle i.v. aplikacije i 98,36±21,58μg/g posle aplikacije elektroforezom, odnosno oko 43 puta više. Zaključujeno je da MmNa primenjen elektroforezom postiže statistički veoma značajno više prosečne koncentracije u inflamiranim zglobovima u odnosu na i.v. aplikaciju. Primena MmNa elektroforezom ima značajnu prednost nad i.v. aplikacijom ovog leka ne samo zbog postizanja značajno viših koncentracija u inflamiranim zglobovima, već i zbog izostajanja sistemskih neželjenih reakcija.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The determination of the concentrations of metamizol sodium in inflamed joints of pigs after intravenous and iontophoretic application
T1  - Određivanje koncentracija metamizol-natrijuma u inflamiranim zglobovima svinja posle intravenske i elektroforetske aplikacije
VL  - 60
IS  - 4
SP  - 371
EP  - 379
DO  - 10.2298/AVB1004371D
ER  - 

@article{
author = "Đurđević, S. and Jezdimirović, Milanka and Aleksić, Nevenka and Branković, Nataša and Pržulj, D. and Cicović, B.",
year = "2010",
abstract = "Concentrations of the NSAID metamizol sodium (MmNa) in the synovial fluid and hyaline cartilage of inflamed knee and elbow joints of pigs after i.v. application and iontophoresis (IPh) were investigated. The research was conducted on 14 male pigs divided equally into two experimental groups and exposed to artificial inflammation of knees and elbows prior to the application of MmNa. The first group (A) was administered 2.5 g (twice the maximum therapeutic dose) MmNa intravenously, whilst the second group (B) was exposed to the same dose, but applied by IPh into contralateral knee and elbow joints. Four hours after the application of MmNa biopsies of the affected knee and elbow joints were performed. The average concentration of MmNa in the synovial fluid of inflamed joints in group A was 9.81±1.96μg/g, while in group B was 170.66±2.07 μg/g, being 17 times higher. The average concentration of MmNa in the hyaline cartilage was 2.29±1.16μg/g following i.v. administration and 98.36±21.58μg/g after IPh, i.e. approximatelly 43 times higher. This led to the conclusion that IPh of MmNa, resulting in incomparably higher concentrations in inflamed joints without any adverse systemic effects, has an advantage over i.v. application., U sinovijalnoj tečnosti i hijalinoj hrskavici inflamiranog kolenog i lakatnog zgloba svinja određivane su koncentracije metamizol-natrijuma (MmNa) aplikovanog i.v. i putem elektroforeze. Arteficijelno zapaljenje kolena i lakta prasadi izazvano je terpentinskim uljem. Metamizol-natrijum je primenjen u dozi od 2.5 g u prvoj grupi prasadi intravenski, a u drugoj putem elektroforeze u kontalateralni koleni i lakatni zglob. Četiri sata posle aplikacije MmNa urađena je biopsija tretiranih zglobova. Prosečna koncentracija MmNa u sinovijalnoj tečnosti inflamiranih zglobova prasadi posle i.v. aplikacije iznosila je 9,81±1,96μg/g, a posle elektroforeze bila je 170,66±2,07μg/g tkiva, odnosno oko 17 puta viša. Izmerena prosečna koncentracija MmNa u hijalinoj hrskavici iznosila je 2,29±1,16μg/g posle i.v. aplikacije i 98,36±21,58μg/g posle aplikacije elektroforezom, odnosno oko 43 puta više. Zaključujeno je da MmNa primenjen elektroforezom postiže statistički veoma značajno više prosečne koncentracije u inflamiranim zglobovima u odnosu na i.v. aplikaciju. Primena MmNa elektroforezom ima značajnu prednost nad i.v. aplikacijom ovog leka ne samo zbog postizanja značajno viših koncentracija u inflamiranim zglobovima, već i zbog izostajanja sistemskih neželjenih reakcija.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The determination of the concentrations of metamizol sodium in inflamed joints of pigs after intravenous and iontophoretic application, Određivanje koncentracija metamizol-natrijuma u inflamiranim zglobovima svinja posle intravenske i elektroforetske aplikacije",
volume = "60",
number = "4",
pages = "371-379",
doi = "10.2298/AVB1004371D"
}

Đurđević, S., Jezdimirović, M., Aleksić, N., Branković, N., Pržulj, D.,& Cicović, B.. (2010). The determination of the concentrations of metamizol sodium in inflamed joints of pigs after intravenous and iontophoretic application. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(4), 371-379.
https://doi.org/10.2298/AVB1004371D

Đurđević S, Jezdimirović M, Aleksić N, Branković N, Pržulj D, Cicović B. The determination of the concentrations of metamizol sodium in inflamed joints of pigs after intravenous and iontophoretic application. in Acta Veterinaria-Beograd. 2010;60(4):371-379.
doi:10.2298/AVB1004371D .

Đurđević, S., Jezdimirović, Milanka, Aleksić, Nevenka, Branković, Nataša, Pržulj, D., Cicović, B., "The determination of the concentrations of metamizol sodium in inflamed joints of pigs after intravenous and iontophoretic application" in Acta Veterinaria-Beograd, 60, no. 4 (2010):371-379,
https://doi.org/10.2298/AVB1004371D . .

TY  - JOUR
AU  - Đurđević, S.
AU  - Jezdimirović, Milanka
AU  - Đurović, A.
AU  - Dedić, Gordana
AU  - Aleksić, Nevenka
AU  - Branković, Nataša
AU  - Stojiljković, S.
AU  - Stojšić, D.
AU  - Blagojević, Z.
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/569
AB  - The current clinical research on pharmacokinetics of methylprednisolone was performed on male pigs to whom was administered either intravenously or locally, via iontophoresis. Equal doses of methylprednisolone sodium succinate (MPSS) were applied, i.e. 40 mg it total per animal. In all pigs artificial inflammation of knee and elbow joints was provoked four days prior to the treatment. Four hours after the application of methylprednisolone tissue samples (both synovial fluid and hyaline cartilage) were obtained from the inflamed joints and subjected to analysis. The quantification of the drug was performed by HPLC technique. The results indicated high quantities of methylprednisolone both in the synovial fluid and hyaline cartilage, the concentrations being significantly higher in animals after iontophoretic application (17.15±3.11 and 12.70±2.19 μg/g, respectively) in comparison with the animals treated intravenously (0.33±0.11 and 0.21±0.06 μg/g, respectively). Thus, iontophoresis was proved a highly advisable clinical means of application of methylprednisolone, especially having in mind the possibility of avoiding systemic adverse effects which are present after parenteral drug administration. In addition, it enables higher therapeutic concentrations of MPSS to be obtained both in the synovial fluid and in the hyaline cartilage of the treated inflamed joints.
AB  - Ispitivana je farmakokinetika metil-prednizolona na svinjama muškog pola kojima je ovaj lek aplikovan intravenski ili lokalno, putem elektroforeze. Metilprednizolon-natrijum-sukcinat primenjen je jednokratno, u dozi od 40 mg po životinji. Četiri dana pre tretmana, kod svih svinja je izazvano arteficijelno zapaljenje kolenog i lakatnog zgloba. Četiri sata posle aplikacije metil-prednizolona uzorci tkiva (sinovijalna tečnost i hijalina hrskavica) uzeti su iz inflamiranih zglobova i zatim analizirani. Kvantifikacija metil-prednizolona obavljena je HPLC metodom. Rezultati su ukazali na prisustvo visokih koncentracija metil-prednizolona u sinovijalnoj tečnosti i hijalinoj hrskavici, koje su bile statistički signifikantno veće posle elektroforetske aplikacije (17,15 ± 3,11 u sinovijalnoj tečnosti i 12,70 ± 2,19 μg/g u hrskavici) u poređenju sa koncentracijama dobijenim posle intravenske primene (0,33 ± 0,11 u sinovijalnoj tečnosti i 0,21 ± 0,06 μg/g u hrskavičnom tkivu). Iz ovog razloga, elektroforeza se preporučuje kao način lokalne aplikacije metilprednizolona kod inflamacije zglobova, naročito kada se ima u vidu mogućnost da se time izbegavaju sistemski neželjeni efekti koji nastaju posle parenteralne primene. Elektroforeza obezbeđuje vrlo visoke terapijske koncentracije metilprednizolon-natrijum-sukcinata kako u sinovijalnoj tečnosti, tako i u hrskavici tretiranih inflamiranih zglobova svinja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The evaluation of the concentrations of methylprednisolone applied intravenously and by iontophoresis in the pig
T1  - Evaluacija koncentracije metil-prednizolona posle intravenske i elektroforetske aplikacije kod domaće svinje
VL  - 59
IS  - 2-3
SP  - 157
EP  - 165
DO  - 10.2298/AVB0903157D
ER  - 

@article{
author = "Đurđević, S. and Jezdimirović, Milanka and Đurović, A. and Dedić, Gordana and Aleksić, Nevenka and Branković, Nataša and Stojiljković, S. and Stojšić, D. and Blagojević, Z.",
year = "2009",
abstract = "The current clinical research on pharmacokinetics of methylprednisolone was performed on male pigs to whom was administered either intravenously or locally, via iontophoresis. Equal doses of methylprednisolone sodium succinate (MPSS) were applied, i.e. 40 mg it total per animal. In all pigs artificial inflammation of knee and elbow joints was provoked four days prior to the treatment. Four hours after the application of methylprednisolone tissue samples (both synovial fluid and hyaline cartilage) were obtained from the inflamed joints and subjected to analysis. The quantification of the drug was performed by HPLC technique. The results indicated high quantities of methylprednisolone both in the synovial fluid and hyaline cartilage, the concentrations being significantly higher in animals after iontophoretic application (17.15±3.11 and 12.70±2.19 μg/g, respectively) in comparison with the animals treated intravenously (0.33±0.11 and 0.21±0.06 μg/g, respectively). Thus, iontophoresis was proved a highly advisable clinical means of application of methylprednisolone, especially having in mind the possibility of avoiding systemic adverse effects which are present after parenteral drug administration. In addition, it enables higher therapeutic concentrations of MPSS to be obtained both in the synovial fluid and in the hyaline cartilage of the treated inflamed joints., Ispitivana je farmakokinetika metil-prednizolona na svinjama muškog pola kojima je ovaj lek aplikovan intravenski ili lokalno, putem elektroforeze. Metilprednizolon-natrijum-sukcinat primenjen je jednokratno, u dozi od 40 mg po životinji. Četiri dana pre tretmana, kod svih svinja je izazvano arteficijelno zapaljenje kolenog i lakatnog zgloba. Četiri sata posle aplikacije metil-prednizolona uzorci tkiva (sinovijalna tečnost i hijalina hrskavica) uzeti su iz inflamiranih zglobova i zatim analizirani. Kvantifikacija metil-prednizolona obavljena je HPLC metodom. Rezultati su ukazali na prisustvo visokih koncentracija metil-prednizolona u sinovijalnoj tečnosti i hijalinoj hrskavici, koje su bile statistički signifikantno veće posle elektroforetske aplikacije (17,15 ± 3,11 u sinovijalnoj tečnosti i 12,70 ± 2,19 μg/g u hrskavici) u poređenju sa koncentracijama dobijenim posle intravenske primene (0,33 ± 0,11 u sinovijalnoj tečnosti i 0,21 ± 0,06 μg/g u hrskavičnom tkivu). Iz ovog razloga, elektroforeza se preporučuje kao način lokalne aplikacije metilprednizolona kod inflamacije zglobova, naročito kada se ima u vidu mogućnost da se time izbegavaju sistemski neželjeni efekti koji nastaju posle parenteralne primene. Elektroforeza obezbeđuje vrlo visoke terapijske koncentracije metilprednizolon-natrijum-sukcinata kako u sinovijalnoj tečnosti, tako i u hrskavici tretiranih inflamiranih zglobova svinja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The evaluation of the concentrations of methylprednisolone applied intravenously and by iontophoresis in the pig, Evaluacija koncentracije metil-prednizolona posle intravenske i elektroforetske aplikacije kod domaće svinje",
volume = "59",
number = "2-3",
pages = "157-165",
doi = "10.2298/AVB0903157D"
}

Đurđević, S., Jezdimirović, M., Đurović, A., Dedić, G., Aleksić, N., Branković, N., Stojiljković, S., Stojšić, D.,& Blagojević, Z.. (2009). The evaluation of the concentrations of methylprednisolone applied intravenously and by iontophoresis in the pig. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(2-3), 157-165.
https://doi.org/10.2298/AVB0903157D

Đurđević S, Jezdimirović M, Đurović A, Dedić G, Aleksić N, Branković N, Stojiljković S, Stojšić D, Blagojević Z. The evaluation of the concentrations of methylprednisolone applied intravenously and by iontophoresis in the pig. in Acta Veterinaria-Beograd. 2009;59(2-3):157-165.
doi:10.2298/AVB0903157D .

Đurđević, S., Jezdimirović, Milanka, Đurović, A., Dedić, Gordana, Aleksić, Nevenka, Branković, Nataša, Stojiljković, S., Stojšić, D., Blagojević, Z., "The evaluation of the concentrations of methylprednisolone applied intravenously and by iontophoresis in the pig" in Acta Veterinaria-Beograd, 59, no. 2-3 (2009):157-165,
https://doi.org/10.2298/AVB0903157D . .