Molecular detection and investigation of the presence of Dirofilariosis causative agent in dogs on the territory of Belgrade
Molekularna detekcija i ispitivanje zastupljenosti uzročnika Dirofilarioze pasa na teritoriji grada Beograda
2016
Аутори
Davitkov, DajanaDavitkov, Darko
Vučićević, Miloš
Nešić, Vladimir
Glavinić, Uroš
Stevanović, Jevrosima
Stanimirović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Dirofilariosis in dogs is a parasitic disease caused by the strains Dirofilaria immitis and D. Repens. Dirofilaria immitis parasitizes in the right side of the heart and pulmonary artery, causing considerable functional impairment of the heart, while adult forms of D. repens mostly parasitize in subcutaneous tissue causing minor skin damage. In this investigation, during the period from May to November 2015, 60 dogs of different race from the territory of Belgrade were examined. Ater the clinical examination, the blood was sampled for microfilariae detection by Knott test, as well as for molecular detection of the agent. Main clinical signs were: fatigue during exersise, shortness of breath and dry cough, anorexia, rapid heartbeat and skin changes. Microfilariae were detected in 22 dogs (37%) by modified Knott test. By molecular genetic method there was determined the prevalence of D. immitis 42%, and D. repens 22%. In this investigation 7 dogs were negative on Knott test, but by PCR m...ethod they showed a positive reaction on the presence of species of the genus Dirofilaria. On the basis of the obtained results, the application of molecular-genetic methods is justified in accurate and reliable species identification of Dirofilaria genus.
Dirofilarioza pasa je parazitsko oboljenje izazvano vrstama Dirofilaria immitis i D. repens. D. immitis parazitira u desnoj strani srca i plućnoj arteriji, dovodeći do značajnog funkcionalnog oštećenja srca, dok adultni oblici D. repens uglavnom parazitiraju u subkutanom tkivu dovodeći do manjih oštećenja kože. U ovom istraživanju ispitano je 60 pasa, različitih rasa, sa teritorije grada Beograda, u periodu od maja do novembra 2015. godine. Nakon kliničkog pregleda, krv je uzorkovana za detekciju mikrofilarija modifikovanim Knott-ovim testom, kao i za molekularnu detekciju uzročnika. Glavni klinički znaci su bili: brzo zamaranje tokom treninga, otežano disanje i suv kašalj, anoreksija, ubrzan rad srca i promene na koži. Mikrofilarije su detektovane kod 22 psa (37%) modifikovanim Knott-ovim testom. Molekularno genetskom metodom utvrđena je prevalencija D.immitis 42%, a D. repens 22 %. U sprovedenom istraživanju je bilo 7 pasa koji su bili negativni na Knott testu, dok su metodom PCR pok...azali pozitivnu reakciju na prisustvo vrsta iz roda Dirofilaria. Na osnovu dobijenih rezultata primena molekularno-genetskih metoda potvrđuje svoju opravdanost u preciznoj i pouzdanoj specijskoj identifikaciji roda Dirofilaria.
Кључне речи:
Belgrade / Dirofilaria spp / Knott test / dog / PCR / Beograd / Dirofilaria spp / Knott test / pas / PCRИзвор:
Veterinarski Glasnik, 2016, 70, 3-4, 89-98Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Davitkov, Dajana AU - Davitkov, Darko AU - Vučićević, Miloš AU - Nešić, Vladimir AU - Glavinić, Uroš AU - Stevanović, Jevrosima AU - Stanimirović, Zoran PY - 2016 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1407 AB - Dirofilariosis in dogs is a parasitic disease caused by the strains Dirofilaria immitis and D. Repens. Dirofilaria immitis parasitizes in the right side of the heart and pulmonary artery, causing considerable functional impairment of the heart, while adult forms of D. repens mostly parasitize in subcutaneous tissue causing minor skin damage. In this investigation, during the period from May to November 2015, 60 dogs of different race from the territory of Belgrade were examined. Ater the clinical examination, the blood was sampled for microfilariae detection by Knott test, as well as for molecular detection of the agent. Main clinical signs were: fatigue during exersise, shortness of breath and dry cough, anorexia, rapid heartbeat and skin changes. Microfilariae were detected in 22 dogs (37%) by modified Knott test. By molecular genetic method there was determined the prevalence of D. immitis 42%, and D. repens 22%. In this investigation 7 dogs were negative on Knott test, but by PCR method they showed a positive reaction on the presence of species of the genus Dirofilaria. On the basis of the obtained results, the application of molecular-genetic methods is justified in accurate and reliable species identification of Dirofilaria genus. AB - Dirofilarioza pasa je parazitsko oboljenje izazvano vrstama Dirofilaria immitis i D. repens. D. immitis parazitira u desnoj strani srca i plućnoj arteriji, dovodeći do značajnog funkcionalnog oštećenja srca, dok adultni oblici D. repens uglavnom parazitiraju u subkutanom tkivu dovodeći do manjih oštećenja kože. U ovom istraživanju ispitano je 60 pasa, različitih rasa, sa teritorije grada Beograda, u periodu od maja do novembra 2015. godine. Nakon kliničkog pregleda, krv je uzorkovana za detekciju mikrofilarija modifikovanim Knott-ovim testom, kao i za molekularnu detekciju uzročnika. Glavni klinički znaci su bili: brzo zamaranje tokom treninga, otežano disanje i suv kašalj, anoreksija, ubrzan rad srca i promene na koži. Mikrofilarije su detektovane kod 22 psa (37%) modifikovanim Knott-ovim testom. Molekularno genetskom metodom utvrđena je prevalencija D.immitis 42%, a D. repens 22 %. U sprovedenom istraživanju je bilo 7 pasa koji su bili negativni na Knott testu, dok su metodom PCR pokazali pozitivnu reakciju na prisustvo vrsta iz roda Dirofilaria. Na osnovu dobijenih rezultata primena molekularno-genetskih metoda potvrđuje svoju opravdanost u preciznoj i pouzdanoj specijskoj identifikaciji roda Dirofilaria. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Veterinarski Glasnik T1 - Molecular detection and investigation of the presence of Dirofilariosis causative agent in dogs on the territory of Belgrade T1 - Molekularna detekcija i ispitivanje zastupljenosti uzročnika Dirofilarioze pasa na teritoriji grada Beograda VL - 70 IS - 3-4 SP - 89 EP - 98 DO - 10.2298/VETGL1604089D ER -
@article{ author = "Davitkov, Dajana and Davitkov, Darko and Vučićević, Miloš and Nešić, Vladimir and Glavinić, Uroš and Stevanović, Jevrosima and Stanimirović, Zoran", year = "2016", abstract = "Dirofilariosis in dogs is a parasitic disease caused by the strains Dirofilaria immitis and D. Repens. Dirofilaria immitis parasitizes in the right side of the heart and pulmonary artery, causing considerable functional impairment of the heart, while adult forms of D. repens mostly parasitize in subcutaneous tissue causing minor skin damage. In this investigation, during the period from May to November 2015, 60 dogs of different race from the territory of Belgrade were examined. Ater the clinical examination, the blood was sampled for microfilariae detection by Knott test, as well as for molecular detection of the agent. Main clinical signs were: fatigue during exersise, shortness of breath and dry cough, anorexia, rapid heartbeat and skin changes. Microfilariae were detected in 22 dogs (37%) by modified Knott test. By molecular genetic method there was determined the prevalence of D. immitis 42%, and D. repens 22%. In this investigation 7 dogs were negative on Knott test, but by PCR method they showed a positive reaction on the presence of species of the genus Dirofilaria. On the basis of the obtained results, the application of molecular-genetic methods is justified in accurate and reliable species identification of Dirofilaria genus., Dirofilarioza pasa je parazitsko oboljenje izazvano vrstama Dirofilaria immitis i D. repens. D. immitis parazitira u desnoj strani srca i plućnoj arteriji, dovodeći do značajnog funkcionalnog oštećenja srca, dok adultni oblici D. repens uglavnom parazitiraju u subkutanom tkivu dovodeći do manjih oštećenja kože. U ovom istraživanju ispitano je 60 pasa, različitih rasa, sa teritorije grada Beograda, u periodu od maja do novembra 2015. godine. Nakon kliničkog pregleda, krv je uzorkovana za detekciju mikrofilarija modifikovanim Knott-ovim testom, kao i za molekularnu detekciju uzročnika. Glavni klinički znaci su bili: brzo zamaranje tokom treninga, otežano disanje i suv kašalj, anoreksija, ubrzan rad srca i promene na koži. Mikrofilarije su detektovane kod 22 psa (37%) modifikovanim Knott-ovim testom. Molekularno genetskom metodom utvrđena je prevalencija D.immitis 42%, a D. repens 22 %. U sprovedenom istraživanju je bilo 7 pasa koji su bili negativni na Knott testu, dok su metodom PCR pokazali pozitivnu reakciju na prisustvo vrsta iz roda Dirofilaria. Na osnovu dobijenih rezultata primena molekularno-genetskih metoda potvrđuje svoju opravdanost u preciznoj i pouzdanoj specijskoj identifikaciji roda Dirofilaria.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Veterinarski Glasnik", title = "Molecular detection and investigation of the presence of Dirofilariosis causative agent in dogs on the territory of Belgrade, Molekularna detekcija i ispitivanje zastupljenosti uzročnika Dirofilarioze pasa na teritoriji grada Beograda", volume = "70", number = "3-4", pages = "89-98", doi = "10.2298/VETGL1604089D" }
Davitkov, D., Davitkov, D., Vučićević, M., Nešić, V., Glavinić, U., Stevanović, J.,& Stanimirović, Z.. (2016). Molecular detection and investigation of the presence of Dirofilariosis causative agent in dogs on the territory of Belgrade. in Veterinarski Glasnik Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(3-4), 89-98. https://doi.org/10.2298/VETGL1604089D
Davitkov D, Davitkov D, Vučićević M, Nešić V, Glavinić U, Stevanović J, Stanimirović Z. Molecular detection and investigation of the presence of Dirofilariosis causative agent in dogs on the territory of Belgrade. in Veterinarski Glasnik. 2016;70(3-4):89-98. doi:10.2298/VETGL1604089D .
Davitkov, Dajana, Davitkov, Darko, Vučićević, Miloš, Nešić, Vladimir, Glavinić, Uroš, Stevanović, Jevrosima, Stanimirović, Zoran, "Molecular detection and investigation of the presence of Dirofilariosis causative agent in dogs on the territory of Belgrade" in Veterinarski Glasnik, 70, no. 3-4 (2016):89-98, https://doi.org/10.2298/VETGL1604089D . .