Homologous Seminal Plasma and Glutathione Promote Pre-capacitation Motility and Structural Stability of Cryopreserved Ram Spermatozoa
2019
Аутори
Nikolovski, M.Dovenska, M.
Ilievska, K.
Adamov, N.
Atanasov, Branko
Radeski, Miroslav
Kirovski, Danijela
Petkov, V.
Dovenski, T.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Reduced glutathione (GSH) and homologous ram seminal plasma (HSP), used as additives in cryopreserving (CP) media prior to freezing, showed conflicting results in retaining structural integrity and progressive motility in post-thawed ram spermatozoa. The aims of this research were (1) to assess the effect of GSH and/or HSP supplementation via soybean-lecithin CP extender on cryopreserved ram spermatozoa viability, morphology and motility pattern; and (2) to assess the effect of incubation in the context of the previous aim. Quantitatively and qualitatively, homogenized and pooled ram ejaculates (N=10) were extended with one of the following extenders Control (C)-tris-based, GSH and HSP-free, experimental-1 (E1)-C + GSH 5 mM, experimental-2 (E2)-C + HSP 20 % and experimental-3 (E3)-GSH 5 mM + HSP 20 %. Following thawing, samples were taken at 0-A nd 3-hours from each group (n=10) and were assessed for spermatozoa viability, morphology, and motility pattern. C-0h samples yielded a sperma...tozoa population with low viability, altered head morphology and highly deviated motility pattern. E3-3h samples yielded spermatozoa with unaffected viability, head morphology and high progressive motility. In conclusion, E3 extender added to cryopreserved-thawed ram spermatozoa is most efficient in obtaining high viability, unaltered head morphology, and progressive motility.
Кључне речи:
cryopreservation / ejaculates / homologous seminal plasma / ram / reduced glutathioneИзвор:
Macedonian Veterinary Review, 2019Издавач:
- Sciendo
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Nikolovski, M. AU - Dovenska, M. AU - Ilievska, K. AU - Adamov, N. AU - Atanasov, Branko AU - Radeski, Miroslav AU - Kirovski, Danijela AU - Petkov, V. AU - Dovenski, T. PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1717 AB - Reduced glutathione (GSH) and homologous ram seminal plasma (HSP), used as additives in cryopreserving (CP) media prior to freezing, showed conflicting results in retaining structural integrity and progressive motility in post-thawed ram spermatozoa. The aims of this research were (1) to assess the effect of GSH and/or HSP supplementation via soybean-lecithin CP extender on cryopreserved ram spermatozoa viability, morphology and motility pattern; and (2) to assess the effect of incubation in the context of the previous aim. Quantitatively and qualitatively, homogenized and pooled ram ejaculates (N=10) were extended with one of the following extenders Control (C)-tris-based, GSH and HSP-free, experimental-1 (E1)-C + GSH 5 mM, experimental-2 (E2)-C + HSP 20 % and experimental-3 (E3)-GSH 5 mM + HSP 20 %. Following thawing, samples were taken at 0-A nd 3-hours from each group (n=10) and were assessed for spermatozoa viability, morphology, and motility pattern. C-0h samples yielded a spermatozoa population with low viability, altered head morphology and highly deviated motility pattern. E3-3h samples yielded spermatozoa with unaffected viability, head morphology and high progressive motility. In conclusion, E3 extender added to cryopreserved-thawed ram spermatozoa is most efficient in obtaining high viability, unaltered head morphology, and progressive motility. PB - Sciendo T2 - Macedonian Veterinary Review T1 - Homologous Seminal Plasma and Glutathione Promote Pre-capacitation Motility and Structural Stability of Cryopreserved Ram Spermatozoa DO - 10.2478/macvetrev-2019-0022 ER -
@article{ author = "Nikolovski, M. and Dovenska, M. and Ilievska, K. and Adamov, N. and Atanasov, Branko and Radeski, Miroslav and Kirovski, Danijela and Petkov, V. and Dovenski, T.", year = "2019", abstract = "Reduced glutathione (GSH) and homologous ram seminal plasma (HSP), used as additives in cryopreserving (CP) media prior to freezing, showed conflicting results in retaining structural integrity and progressive motility in post-thawed ram spermatozoa. The aims of this research were (1) to assess the effect of GSH and/or HSP supplementation via soybean-lecithin CP extender on cryopreserved ram spermatozoa viability, morphology and motility pattern; and (2) to assess the effect of incubation in the context of the previous aim. Quantitatively and qualitatively, homogenized and pooled ram ejaculates (N=10) were extended with one of the following extenders Control (C)-tris-based, GSH and HSP-free, experimental-1 (E1)-C + GSH 5 mM, experimental-2 (E2)-C + HSP 20 % and experimental-3 (E3)-GSH 5 mM + HSP 20 %. Following thawing, samples were taken at 0-A nd 3-hours from each group (n=10) and were assessed for spermatozoa viability, morphology, and motility pattern. C-0h samples yielded a spermatozoa population with low viability, altered head morphology and highly deviated motility pattern. E3-3h samples yielded spermatozoa with unaffected viability, head morphology and high progressive motility. In conclusion, E3 extender added to cryopreserved-thawed ram spermatozoa is most efficient in obtaining high viability, unaltered head morphology, and progressive motility.", publisher = "Sciendo", journal = "Macedonian Veterinary Review", title = "Homologous Seminal Plasma and Glutathione Promote Pre-capacitation Motility and Structural Stability of Cryopreserved Ram Spermatozoa", doi = "10.2478/macvetrev-2019-0022" }
Nikolovski, M., Dovenska, M., Ilievska, K., Adamov, N., Atanasov, B., Radeski, M., Kirovski, D., Petkov, V.,& Dovenski, T.. (2019). Homologous Seminal Plasma and Glutathione Promote Pre-capacitation Motility and Structural Stability of Cryopreserved Ram Spermatozoa. in Macedonian Veterinary Review Sciendo.. https://doi.org/10.2478/macvetrev-2019-0022
Nikolovski M, Dovenska M, Ilievska K, Adamov N, Atanasov B, Radeski M, Kirovski D, Petkov V, Dovenski T. Homologous Seminal Plasma and Glutathione Promote Pre-capacitation Motility and Structural Stability of Cryopreserved Ram Spermatozoa. in Macedonian Veterinary Review. 2019;. doi:10.2478/macvetrev-2019-0022 .
Nikolovski, M., Dovenska, M., Ilievska, K., Adamov, N., Atanasov, Branko, Radeski, Miroslav, Kirovski, Danijela, Petkov, V., Dovenski, T., "Homologous Seminal Plasma and Glutathione Promote Pre-capacitation Motility and Structural Stability of Cryopreserved Ram Spermatozoa" in Macedonian Veterinary Review (2019), https://doi.org/10.2478/macvetrev-2019-0022 . .