TY  - CONF
AU  - Galić, Ivan
AU  - Stančić, Ivan
AU  - Apić, Jelena
AU  - Maletić, Milan
AU  - Barna, Tomislav
AU  - Vračar, Vuk
AU  - Rodić, Stevan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3268
AB  - Reprodukcija svinja danas predstavlja osetljivo polje delovanja veterinarske službe koje
značajno utiče na profitabilnost svinjarske proizvodnje. Bez obzira na ovu činjenicu, neki
standardni pregledi razređenog semena nerastova kroz duži vremenski period predstavljaju
nezamenljiv način kontrole kvaliteta dobijenog ejakulata. Cito-morfološki pregled izvodi se u
svrhu procene učestalosti pojavljivanja abnormalno građenih spermatozoida. Citoplazmaske kapi
javljaju se na srednjem delu spermatozoida, a po mestu lokacije, dele se na proksimalne i distalne.
Distalne citoplazmatske kapi ukazuju na nezrelost spermatozoida, rano uvođenje mladih
nerastova u reproduktivnu eksploataciju, prekomerno iskorišćavanje priplodnjaka ili pak
posledično pojavljivanje ovakvih spermatozoida kao odgovor na ambijentalne oscilacije ili druge
zdravstvene probleme vezane za nerasta. Ovaj rad predstavlja istraživanje koje je sprovedeno na
nerastovima (n=30), durok i landras rasne pripadnosti. Eksperimentalna grupa životinja dobijala
je peroralno zajedno sa kompletnom smešom za ishranu, dodatak preparata sa antioksidativnim
potencijalom. Za potrebe bojenja spermatozoida korišćena je modifikovana metoda ‚‚u jednom
koraku” (Mortimer, 1994; Jovičin i sar., 2012). Primena dvofaktorske ANOVE za ponovljena
merenja pokazala je da nema statistički značajne razlike između kontrolne i eksperimentalne
grupe nerastova u nalazima citoplazmatskih kapi spermatozoida (%) (F(7, 112)= 1,65, p= 0,13) u
odabranim vremenskim tačkama. Iako nije zabeležena statistički značajna razlika, ovakav
rezultat, odnosno konstanta u broju ovakvih spermatozoida nerastova označava i ujednačenost
uslova pod kojim je ekseriment sproveden.
PB  - Požarevac : Sitograf RM
C3  - 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023
T1  - Citoplazmatske kapi spermatozoida nerastova – abnormalnost ili fiziološka pojava?
SP  - 174
EP  - 181
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3268
ER  - 

@conference{
author = "Galić, Ivan and Stančić, Ivan and Apić, Jelena and Maletić, Milan and Barna, Tomislav and Vračar, Vuk and Rodić, Stevan",
year = "2023",
abstract = "Reprodukcija svinja danas predstavlja osetljivo polje delovanja veterinarske službe koje
značajno utiče na profitabilnost svinjarske proizvodnje. Bez obzira na ovu činjenicu, neki
standardni pregledi razređenog semena nerastova kroz duži vremenski period predstavljaju
nezamenljiv način kontrole kvaliteta dobijenog ejakulata. Cito-morfološki pregled izvodi se u
svrhu procene učestalosti pojavljivanja abnormalno građenih spermatozoida. Citoplazmaske kapi
javljaju se na srednjem delu spermatozoida, a po mestu lokacije, dele se na proksimalne i distalne.
Distalne citoplazmatske kapi ukazuju na nezrelost spermatozoida, rano uvođenje mladih
nerastova u reproduktivnu eksploataciju, prekomerno iskorišćavanje priplodnjaka ili pak
posledično pojavljivanje ovakvih spermatozoida kao odgovor na ambijentalne oscilacije ili druge
zdravstvene probleme vezane za nerasta. Ovaj rad predstavlja istraživanje koje je sprovedeno na
nerastovima (n=30), durok i landras rasne pripadnosti. Eksperimentalna grupa životinja dobijala
je peroralno zajedno sa kompletnom smešom za ishranu, dodatak preparata sa antioksidativnim
potencijalom. Za potrebe bojenja spermatozoida korišćena je modifikovana metoda ‚‚u jednom
koraku” (Mortimer, 1994; Jovičin i sar., 2012). Primena dvofaktorske ANOVE za ponovljena
merenja pokazala je da nema statistički značajne razlike između kontrolne i eksperimentalne
grupe nerastova u nalazima citoplazmatskih kapi spermatozoida (%) (F(7, 112)= 1,65, p= 0,13) u
odabranim vremenskim tačkama. Iako nije zabeležena statistički značajna razlika, ovakav
rezultat, odnosno konstanta u broju ovakvih spermatozoida nerastova označava i ujednačenost
uslova pod kojim je ekseriment sproveden.",
publisher = "Požarevac : Sitograf RM",
journal = "20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023",
title = "Citoplazmatske kapi spermatozoida nerastova – abnormalnost ili fiziološka pojava?",
pages = "174-181",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3268"
}

Galić, I., Stančić, I., Apić, J., Maletić, M., Barna, T., Vračar, V.,& Rodić, S.. (2023). Citoplazmatske kapi spermatozoida nerastova – abnormalnost ili fiziološka pojava?. in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023
Požarevac : Sitograf RM., 174-181.
https://hdl.handle.net/21.15107/rcub_veterinar_3268

Galić I, Stančić I, Apić J, Maletić M, Barna T, Vračar V, Rodić S. Citoplazmatske kapi spermatozoida nerastova – abnormalnost ili fiziološka pojava?. in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023. 2023;:174-181.
https://hdl.handle.net/21.15107/rcub_veterinar_3268 .

Galić, Ivan, Stančić, Ivan, Apić, Jelena, Maletić, Milan, Barna, Tomislav, Vračar, Vuk, Rodić, Stevan, "Citoplazmatske kapi spermatozoida nerastova – abnormalnost ili fiziološka pojava?" in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023 (2023):174-181,
https://hdl.handle.net/21.15107/rcub_veterinar_3268 .

TY  - CONF
AU  - Galić, Ivan
AU  - Stančić, Ivan
AU  - Maletić, Milan
AU  - Apić, Jelena
AU  - Barna, Tomislav
AU  - Rodić, Stevan
AU  - Ristevski, Dragan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3214
AB  - Nizak antioksidativni kapacitet spermatozoida nerastova, često može biti i jedan
od glavnih razloga lošeg kvaliteta semena. Iz ove činjenice proizilazi i veoma velika
podložnost spermatozoida oksidativnom stresu i različitim štetnim efektima. Kada
proizvodnja reaktivnih vrsta kiseonika (ROS) nadmaši unutrašnju antioksidativnu
zaštitu, dolazi do probijanja sistema biološke odbrane, što za posledicu ima oštećenje
ćelije. Narušena redoks homeostaza ćelije, usled prekomernog stvaranja, ali i
neadekvatnog uklanjanja slobodnih radikala dovodi do oksidativnog stresa. Napad
reaktivnih vrsta kiseonika dovodi do smanjene pokretljivosti spermatozoida,
smanjene vitalnosti spermatozoida i povećanja morfoloških abnormalnosti
spermatozoida sa štetnim efektima po kapacitaciju spermatozoida i akrozomalnu
reakciju. Sa druge strane, niske koncentracije reaktivnih vrsta kiseonika igraju važnu
ulogu u fiziološkim procesima kao što su kapacitacija spermatozoida, reakcija
akrozoma i fertilizacija. Veća koncentracija antioksidativnih molekula u spermalnoj
plazmi, kompenzuje nizak antioksidativni kapacitet samih spermatozoida nerastova.
Sistem antioksidativne zaštite sastoji se od enzima koji uklanjaju slobodne radikale,
neenzimskih komponenti, specifičnih oksidoreduktaza i proteina. U membrani,
citoplazmi i organelama spermatozoida i spermalnoj plazmi, nalaze se enzimi
supreroksid dismutaza, glutation peroksidaza, glutation reduktaza, katalaza, među
kojima se superoksid dismutaza smatra najvažnijim antioksidativnim enzimom. Cilj
ovog rada je da na osnovu pregleda literature prikaže negativan efekat oksidativnog
stresa, ali i sistem antioksidativne zaštite koju poseduje sprmalna plazma nerastova.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
T1  - Negativan efekat oksidativnog stresa na plodnost priplodnih nerastova
SP  - 69
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3214
ER  - 

@conference{
author = "Galić, Ivan and Stančić, Ivan and Maletić, Milan and Apić, Jelena and Barna, Tomislav and Rodić, Stevan and Ristevski, Dragan",
year = "2023",
abstract = "Nizak antioksidativni kapacitet spermatozoida nerastova, često može biti i jedan
od glavnih razloga lošeg kvaliteta semena. Iz ove činjenice proizilazi i veoma velika
podložnost spermatozoida oksidativnom stresu i različitim štetnim efektima. Kada
proizvodnja reaktivnih vrsta kiseonika (ROS) nadmaši unutrašnju antioksidativnu
zaštitu, dolazi do probijanja sistema biološke odbrane, što za posledicu ima oštećenje
ćelije. Narušena redoks homeostaza ćelije, usled prekomernog stvaranja, ali i
neadekvatnog uklanjanja slobodnih radikala dovodi do oksidativnog stresa. Napad
reaktivnih vrsta kiseonika dovodi do smanjene pokretljivosti spermatozoida,
smanjene vitalnosti spermatozoida i povećanja morfoloških abnormalnosti
spermatozoida sa štetnim efektima po kapacitaciju spermatozoida i akrozomalnu
reakciju. Sa druge strane, niske koncentracije reaktivnih vrsta kiseonika igraju važnu
ulogu u fiziološkim procesima kao što su kapacitacija spermatozoida, reakcija
akrozoma i fertilizacija. Veća koncentracija antioksidativnih molekula u spermalnoj
plazmi, kompenzuje nizak antioksidativni kapacitet samih spermatozoida nerastova.
Sistem antioksidativne zaštite sastoji se od enzima koji uklanjaju slobodne radikale,
neenzimskih komponenti, specifičnih oksidoreduktaza i proteina. U membrani,
citoplazmi i organelama spermatozoida i spermalnoj plazmi, nalaze se enzimi
supreroksid dismutaza, glutation peroksidaza, glutation reduktaza, katalaza, među
kojima se superoksid dismutaza smatra najvažnijim antioksidativnim enzimom. Cilj
ovog rada je da na osnovu pregleda literature prikaže negativan efekat oksidativnog
stresa, ali i sistem antioksidativne zaštite koju poseduje sprmalna plazma nerastova.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023",
title = "Negativan efekat oksidativnog stresa na plodnost priplodnih nerastova",
pages = "69-77",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3214"
}

Galić, I., Stančić, I., Maletić, M., Apić, J., Barna, T., Rodić, S.,& Ristevski, D.. (2023). Negativan efekat oksidativnog stresa na plodnost priplodnih nerastova. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 69-77.
https://hdl.handle.net/21.15107/rcub_veterinar_3214

Galić I, Stančić I, Maletić M, Apić J, Barna T, Rodić S, Ristevski D. Negativan efekat oksidativnog stresa na plodnost priplodnih nerastova. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023. 2023;:69-77.
https://hdl.handle.net/21.15107/rcub_veterinar_3214 .

Galić, Ivan, Stančić, Ivan, Maletić, Milan, Apić, Jelena, Barna, Tomislav, Rodić, Stevan, Ristevski, Dragan, "Negativan efekat oksidativnog stresa na plodnost priplodnih nerastova" in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023 (2023):69-77,
https://hdl.handle.net/21.15107/rcub_veterinar_3214 .

TY  - CONF
AU  - Galić, Ivan
AU  - Stančić, Ivan
AU  - Apić, Jelena
AU  - Maletić, Milan
AU  - Spasojević, Jovan
AU  - Toholj, Bojan
AU  - Todorović, Srđan
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3422
AB  - Svinjarska proizvodnja se u današnjoj praksi ne može zamisliti bez veštačkog
osemenjavanja. Za maksimalnu eksploataciju nerastova uvek mora da se veže dobar kvalitet
ejakulata, kao i dobro zdravstveno stanje nerasta. Spermatozoidi nerastova zbog svog niskog
antioksidativnog kapaciteta kontinuirano su negativno izloženi oksidativnom stresu.
Potencijalna klinička korist za poboljšanje antioksidativne zaštite i smanjivanje
negativnih uticaja na spermatozoide, od strane suplementnih preparata, i dalje izaziva različita i
podeljena mišljenja u naučnim krugovima. Oksidativni stres nastaje kada unutrašnja
antioksidativna odbrana biološkog sistema biva nadvladana od prekomernog nakupljanja/
proizvodnje reaktivnih vrsta kiseonika.
Preliminarna istraživanja ove grupe autora na uzorku od 30 nerastova, gde je
eksperimentalna grupa dobijala kroz ishranu dodatak organskog selena i preparata Oxynat 3D,
govore u prilog da i ova kombinacija može potencijalno poboljšati kvalitet ejakulata. Ispitivani
nerastovi bili su rase durok i landras, prosečno stari godinu dana. Ispitivanje kvaliteta ejakulata
vršeno je ciklusno u vremenskom trajanju jedne spermatogeneze.
Kvalitet razređenog semena je određivan metodom CASA (Computer Assisted Sperm
Analysis), sa integrisanim softverskim sistemom za analizu spermatozoida, u četiri navrata.
Rezultati pokazuju statistički značajne razlike za ukupnu pokretljivost, volumen ejakulata
i broj preporučenih doza, dok je koncentracija spermatozoida bila bez statistički značajne
razlike. Navedene činjenice jasno ukazuju da kombinacija ova dva preparata, koji imaju
antioksidativni potencijal, dovodi do poboljšanja kvaliteta razređene sperme priplodnih
nerastova.
PB  - Požarevac : Sitograf RM : Veterinarski specijalistički institut
C3  - 19. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 2 - 3 jun, 2022
T1  - Poboljšanje kvaliteta razređene sperme nerastova upotrebom antioksidansnih suplemenata - preliminarna procena
SP  - 26
EP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3422
ER  - 

@conference{
author = "Galić, Ivan and Stančić, Ivan and Apić, Jelena and Maletić, Milan and Spasojević, Jovan and Toholj, Bojan and Todorović, Srđan",
year = "2022",
abstract = "Svinjarska proizvodnja se u današnjoj praksi ne može zamisliti bez veštačkog
osemenjavanja. Za maksimalnu eksploataciju nerastova uvek mora da se veže dobar kvalitet
ejakulata, kao i dobro zdravstveno stanje nerasta. Spermatozoidi nerastova zbog svog niskog
antioksidativnog kapaciteta kontinuirano su negativno izloženi oksidativnom stresu.
Potencijalna klinička korist za poboljšanje antioksidativne zaštite i smanjivanje
negativnih uticaja na spermatozoide, od strane suplementnih preparata, i dalje izaziva različita i
podeljena mišljenja u naučnim krugovima. Oksidativni stres nastaje kada unutrašnja
antioksidativna odbrana biološkog sistema biva nadvladana od prekomernog nakupljanja/
proizvodnje reaktivnih vrsta kiseonika.
Preliminarna istraživanja ove grupe autora na uzorku od 30 nerastova, gde je
eksperimentalna grupa dobijala kroz ishranu dodatak organskog selena i preparata Oxynat 3D,
govore u prilog da i ova kombinacija može potencijalno poboljšati kvalitet ejakulata. Ispitivani
nerastovi bili su rase durok i landras, prosečno stari godinu dana. Ispitivanje kvaliteta ejakulata
vršeno je ciklusno u vremenskom trajanju jedne spermatogeneze.
Kvalitet razređenog semena je određivan metodom CASA (Computer Assisted Sperm
Analysis), sa integrisanim softverskim sistemom za analizu spermatozoida, u četiri navrata.
Rezultati pokazuju statistički značajne razlike za ukupnu pokretljivost, volumen ejakulata
i broj preporučenih doza, dok je koncentracija spermatozoida bila bez statistički značajne
razlike. Navedene činjenice jasno ukazuju da kombinacija ova dva preparata, koji imaju
antioksidativni potencijal, dovodi do poboljšanja kvaliteta razređene sperme priplodnih
nerastova.",
publisher = "Požarevac : Sitograf RM : Veterinarski specijalistički institut",
journal = "19. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 2 - 3 jun, 2022",
title = "Poboljšanje kvaliteta razređene sperme nerastova upotrebom antioksidansnih suplemenata - preliminarna procena",
pages = "26-26",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3422"
}

Galić, I., Stančić, I., Apić, J., Maletić, M., Spasojević, J., Toholj, B.,& Todorović, S.. (2022). Poboljšanje kvaliteta razređene sperme nerastova upotrebom antioksidansnih suplemenata - preliminarna procena. in 19. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 2 - 3 jun, 2022
Požarevac : Sitograf RM : Veterinarski specijalistički institut., 26-26.
https://hdl.handle.net/21.15107/rcub_veterinar_3422

Galić I, Stančić I, Apić J, Maletić M, Spasojević J, Toholj B, Todorović S. Poboljšanje kvaliteta razređene sperme nerastova upotrebom antioksidansnih suplemenata - preliminarna procena. in 19. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 2 - 3 jun, 2022. 2022;:26-26.
https://hdl.handle.net/21.15107/rcub_veterinar_3422 .

Galić, Ivan, Stančić, Ivan, Apić, Jelena, Maletić, Milan, Spasojević, Jovan, Toholj, Bojan, Todorović, Srđan, "Poboljšanje kvaliteta razređene sperme nerastova upotrebom antioksidansnih suplemenata - preliminarna procena" in 19. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 2 - 3 jun, 2022 (2022):26-26,
https://hdl.handle.net/21.15107/rcub_veterinar_3422 .

TY  - JOUR
AU  - Jakovljević, Goran
AU  - Lazarević, Miodrag
AU  - Mirilović, Milorad
AU  - Milovanović, Aleksandar
AU  - Apić, Jelena
AU  - Šefer, Dragan
AU  - Nedić, Svetlana
AU  - Vakanjac, Slobodanka
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2267
AB  - This study aimed to assess the influence of different concentrations of zinc (Zn), lead (Pb), mercury (Hg) and cadmium (Cd) found in the feed and water provided to bulls on the concentrations of these metals in cryopreserved bull semen, and to determine their influence onto semen quality parameters. Correlations between heavy metal concentrations in the semen and the quality parameters of semen as estimated by computer-assisted sperm analysis (CASA) and flow cytometry (FC) methods were determined. A total of 40 cryopreserved semen samples originating from bulls housed in 4 different centers for artificial insemination (A, B, C and D) were examined, making a total of 160 samples. The concentrations of metals and semen quality parameters were determined in cryopreserved semen of 10 bulls from each center, namely 4 samples from each bull. Concentrations of Zn, Pb, Hg and Cd in hay and concentrated feed were within the allowed limits as proposed by the National Research Council (NRC, 2000). A strong negative correlation was detected between curvilinear velocity (VCL) and Zn concentrations in the semen (P < 0.01; r = -0.772) in group D, and a positive correlation of VCL with Pb concentrations (P < 0.05 and r = 0.718) in group B. Mercury concentrations in cryopreserved semen correlated negatively to the percent of live sperm cells with intact acrosomes (V/IA: P < 0.05; r = -0.640) and positively with the percent of dead sperm cells with damaged acrosomes (D/DA: P < 0.01; r = 0.766) in group D. This finding confirms the hypothesis that Hg, even at low concentrations, may cause acrosome damage.
AB  - Cilj rada bio je da se ispita uticaj različitih koncentracija cinka (Zn), olova (Pb), žive
(Hg) i kadmijuma (Cd) iz hrane i vode na koncentracije ovih metala u duboko zamrznutom semenu nakon otapanja i da se ispita njihov uticaj na parametre kvaliteta
semena. Korelacije između koncentracije teških metala u semenu i parametra kvaliteta semena su određivane nakon ispitivanja semena kompjuterski asistiranom analizom
semena (eng. computer-assisted sperm analysis - CASA) i protočnom citometrijom
(eng. flow cytometry - FC).
Iz 4 različita centra za veštačko osemenjavanje (A, B, C i D) ispitivano je po 40 uzoraka duboko zamrznutog semena bikova, što čini ukupno 160 uzoraka. Koncentracije
metala i parametri kvaliteta semena su određivani u duboko zamrznutom semenu od
10 bikova iz svakog centra, odnosno 4 uzorka od svakog bika. Koncentracije Zn, Pb,
Hg i Cd u senu i koncentrovanim hranivima bile su ispod propisanih dozvoljenih
vrednosti Nacionalnog Istraživačkog Veća (eng. National Research Council - NRC,
2000). Jaka negativna korelacija uočena je između krivolinijske brzine spermatozoida
(eng. curvilinear velocity - VCL) i koncentracije Zn u semenu (P < 0.01; r = -0.772) u
grupi D, i pozitivna korelacija između VCL i koncentracije Pb (P < 0.05 and r = 0.718)
u grupi B. Koncentracija žive u duboko zamrznutom semenu negativno je korelirala
sa procentom živih spermatozoida sa neoštećenim akrozomom (eng. live sperm cells
with intact acrosomes - V/IA: P < 0.05; r = -0.640) i pozitivno sa procentom mrtvih
spermatozoida sa oštećenim akrozomom (eng. dead sperm cells with damaged acrosomes - D/DA: P < 0.01; r = 0.766) u grupi D. Ovi rezultati potvrđuju hipotezu da Hg,
čak i u niskim koncenracijama može da prouzrokuje oštećenje akrozoma.
PB  - Fakultet veterinarske medicine
T2  - Acta veterinaria-Beograd
T1  - The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen
T1  - Uticaj cinka i teških metala iz hrane i vode na kvalitet duboko zamrznutog semena bikova
VL  - 71
IS  - 3
SP  - 326
EP  - 343
DO  - 10.2478/acve-2021-0028
ER  - 

@article{
author = "Jakovljević, Goran and Lazarević, Miodrag and Mirilović, Milorad and Milovanović, Aleksandar and Apić, Jelena and Šefer, Dragan and Nedić, Svetlana and Vakanjac, Slobodanka",
year = "2021",
abstract = "This study aimed to assess the influence of different concentrations of zinc (Zn), lead (Pb), mercury (Hg) and cadmium (Cd) found in the feed and water provided to bulls on the concentrations of these metals in cryopreserved bull semen, and to determine their influence onto semen quality parameters. Correlations between heavy metal concentrations in the semen and the quality parameters of semen as estimated by computer-assisted sperm analysis (CASA) and flow cytometry (FC) methods were determined. A total of 40 cryopreserved semen samples originating from bulls housed in 4 different centers for artificial insemination (A, B, C and D) were examined, making a total of 160 samples. The concentrations of metals and semen quality parameters were determined in cryopreserved semen of 10 bulls from each center, namely 4 samples from each bull. Concentrations of Zn, Pb, Hg and Cd in hay and concentrated feed were within the allowed limits as proposed by the National Research Council (NRC, 2000). A strong negative correlation was detected between curvilinear velocity (VCL) and Zn concentrations in the semen (P < 0.01; r = -0.772) in group D, and a positive correlation of VCL with Pb concentrations (P < 0.05 and r = 0.718) in group B. Mercury concentrations in cryopreserved semen correlated negatively to the percent of live sperm cells with intact acrosomes (V/IA: P < 0.05; r = -0.640) and positively with the percent of dead sperm cells with damaged acrosomes (D/DA: P < 0.01; r = 0.766) in group D. This finding confirms the hypothesis that Hg, even at low concentrations, may cause acrosome damage., Cilj rada bio je da se ispita uticaj različitih koncentracija cinka (Zn), olova (Pb), žive
(Hg) i kadmijuma (Cd) iz hrane i vode na koncentracije ovih metala u duboko zamrznutom semenu nakon otapanja i da se ispita njihov uticaj na parametre kvaliteta
semena. Korelacije između koncentracije teških metala u semenu i parametra kvaliteta semena su određivane nakon ispitivanja semena kompjuterski asistiranom analizom
semena (eng. computer-assisted sperm analysis - CASA) i protočnom citometrijom
(eng. flow cytometry - FC).
Iz 4 različita centra za veštačko osemenjavanje (A, B, C i D) ispitivano je po 40 uzoraka duboko zamrznutog semena bikova, što čini ukupno 160 uzoraka. Koncentracije
metala i parametri kvaliteta semena su određivani u duboko zamrznutom semenu od
10 bikova iz svakog centra, odnosno 4 uzorka od svakog bika. Koncentracije Zn, Pb,
Hg i Cd u senu i koncentrovanim hranivima bile su ispod propisanih dozvoljenih
vrednosti Nacionalnog Istraživačkog Veća (eng. National Research Council - NRC,
2000). Jaka negativna korelacija uočena je između krivolinijske brzine spermatozoida
(eng. curvilinear velocity - VCL) i koncentracije Zn u semenu (P < 0.01; r = -0.772) u
grupi D, i pozitivna korelacija između VCL i koncentracije Pb (P < 0.05 and r = 0.718)
u grupi B. Koncentracija žive u duboko zamrznutom semenu negativno je korelirala
sa procentom živih spermatozoida sa neoštećenim akrozomom (eng. live sperm cells
with intact acrosomes - V/IA: P < 0.05; r = -0.640) i pozitivno sa procentom mrtvih
spermatozoida sa oštećenim akrozomom (eng. dead sperm cells with damaged acrosomes - D/DA: P < 0.01; r = 0.766) u grupi D. Ovi rezultati potvrđuju hipotezu da Hg,
čak i u niskim koncenracijama može da prouzrokuje oštećenje akrozoma.",
publisher = "Fakultet veterinarske medicine",
journal = "Acta veterinaria-Beograd",
title = "The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen, Uticaj cinka i teških metala iz hrane i vode na kvalitet duboko zamrznutog semena bikova",
volume = "71",
number = "3",
pages = "326-343",
doi = "10.2478/acve-2021-0028"
}

Jakovljević, G., Lazarević, M., Mirilović, M., Milovanović, A., Apić, J., Šefer, D., Nedić, S.,& Vakanjac, S.. (2021). The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen. in Acta veterinaria-Beograd
Fakultet veterinarske medicine., 71(3), 326-343.
https://doi.org/10.2478/acve-2021-0028

Jakovljević G, Lazarević M, Mirilović M, Milovanović A, Apić J, Šefer D, Nedić S, Vakanjac S. The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen. in Acta veterinaria-Beograd. 2021;71(3):326-343.
doi:10.2478/acve-2021-0028 .

Jakovljević, Goran, Lazarević, Miodrag, Mirilović, Milorad, Milovanović, Aleksandar, Apić, Jelena, Šefer, Dragan, Nedić, Svetlana, Vakanjac, Slobodanka, "The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen" in Acta veterinaria-Beograd, 71, no. 3 (2021):326-343,
https://doi.org/10.2478/acve-2021-0028 . .

TY  - GEN
AU  - Apić, Jelena
AU  - Galić, Ivan
AU  - Stančić, Ivan
AU  - Barna, Tomislav
AU  - Vakanjac, Slobodanka
AU  - Milovanović, Aleksandar
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3160
AB  - Današnje savremeno svinjarstvo ne može da se zamisli bez primene biotehnološke
metode veštačkog osemenjavanja. Efikasno reproduktivno iskorišćavanje, genetski
superiornih nerastova, zahteva formiranje maksimalnog broja inseminacionih doza po
ejakulatu, odnosno po nerastu godišnje. Rezultati brojnih istraživanja dokazuju da bi
proteini spermalne plazme mogli imati značajan uticaj na variranje stepena progresivne
pokretlјivosti spermatozoida i održavanje fertilizacionog kapaciteta razređene
sperme pojedinih nerastova. U naše istraživanje je bilo uključeno 15 nerastova rase
švedski landras (5) veliki jorkšir (5) i durok (5) približne starosti od 18 do 24 meseca,
u punoj proizvodnoj ekploataciji. Od svakog nerasta su uzeta po tri ejakulata (ukupno
45 ejakulata), u razmaku od mesec dana, u hladnijem delu godine (oktobar-novembar-
decembar). Kod svih nerastova je utvrđivan procenat ukupnih proteina u spermalnoj
plazmi. Na osnovu dobijenih rezulata sadržaja ukupnih proteina u spermalnoj plazmi,
ispitivani nerastovi su podeljeni u dve grupe, grupa (N)-(niski): nerastovi sa niskim
sadržajem proteina (≤ 2,0) i grupa (V)-(visoki): nerastovi sa visokim sadržajem proteina
(≥ 2,1). Poređenjem parametara kvaliteta razređenog semena između ove dve grupe,
utvrđeno je da su prosečne vrednosti svih ispitivanih parametara fertilizacionog potencijala
razređenog semena bile statistički značajno veće u grupi V u odnosu na grupu
N, (p<0,01). Tako su prosečne vrednosti za ukupan broj spermatozoida u dozi iznosile
6,293×109 (V) i 3,595×109 (N), ukupan broj pokretnih spermatozoida u dozi 4,771×109
(V) i 1,325×109(N), procenat ukupno pokretnih spermatozoida 74,20 (V) i 36,26 (N),
ukupan broj progresivno pokretnih spermatozoida 2,982×109 (V) i 731,47×106(N), a
procenat progresivno pokretnih spermatozoida je iznosio 47,90 (V) i 21,42 (N). Nisu
ustanovljene statistički značajne (p>0,05) razlike u prosečom sadržaju proteina u spermalnoj
plazmi, između ispitivanih rasa nerastova. Ova vrednost je iznosila 2,43 procenta
kod rase landras, 2,45 kod rase veliki jorkšir i 1,83 kod rase durok. Prosečna vrednost
sadržaja proteina u spermalnoj plazmi nije značajno (p>0,05) varirala ni u zavisnosti od starosti ispitivanih nerastova. Navedene činjenice, ukazuju da sadržaj proteina u
spermalnoj plazmi, može biti efikasan marker fertilizacionog potencijala nerastova, na
osnovu koga se oni mogu selekcionisati za upotrebu u veštačkom osemenjavanju.
PB  - Beograd : Srpsko veterinarsko društvo
T2  - 32. Savetovanje veterinara Srbije, Zlatibor, 9 – 12. septembar 2021
T1  - Proteini spermalne plazme nerastova kao genetski markeri kvaliteta semena
SP  - 92
EP  - 103
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3160
ER  - 

@misc{
author = "Apić, Jelena and Galić, Ivan and Stančić, Ivan and Barna, Tomislav and Vakanjac, Slobodanka and Milovanović, Aleksandar",
year = "2021",
abstract = "Današnje savremeno svinjarstvo ne može da se zamisli bez primene biotehnološke
metode veštačkog osemenjavanja. Efikasno reproduktivno iskorišćavanje, genetski
superiornih nerastova, zahteva formiranje maksimalnog broja inseminacionih doza po
ejakulatu, odnosno po nerastu godišnje. Rezultati brojnih istraživanja dokazuju da bi
proteini spermalne plazme mogli imati značajan uticaj na variranje stepena progresivne
pokretlјivosti spermatozoida i održavanje fertilizacionog kapaciteta razređene
sperme pojedinih nerastova. U naše istraživanje je bilo uključeno 15 nerastova rase
švedski landras (5) veliki jorkšir (5) i durok (5) približne starosti od 18 do 24 meseca,
u punoj proizvodnoj ekploataciji. Od svakog nerasta su uzeta po tri ejakulata (ukupno
45 ejakulata), u razmaku od mesec dana, u hladnijem delu godine (oktobar-novembar-
decembar). Kod svih nerastova je utvrđivan procenat ukupnih proteina u spermalnoj
plazmi. Na osnovu dobijenih rezulata sadržaja ukupnih proteina u spermalnoj plazmi,
ispitivani nerastovi su podeljeni u dve grupe, grupa (N)-(niski): nerastovi sa niskim
sadržajem proteina (≤ 2,0) i grupa (V)-(visoki): nerastovi sa visokim sadržajem proteina
(≥ 2,1). Poređenjem parametara kvaliteta razređenog semena između ove dve grupe,
utvrđeno je da su prosečne vrednosti svih ispitivanih parametara fertilizacionog potencijala
razređenog semena bile statistički značajno veće u grupi V u odnosu na grupu
N, (p<0,01). Tako su prosečne vrednosti za ukupan broj spermatozoida u dozi iznosile
6,293×109 (V) i 3,595×109 (N), ukupan broj pokretnih spermatozoida u dozi 4,771×109
(V) i 1,325×109(N), procenat ukupno pokretnih spermatozoida 74,20 (V) i 36,26 (N),
ukupan broj progresivno pokretnih spermatozoida 2,982×109 (V) i 731,47×106(N), a
procenat progresivno pokretnih spermatozoida je iznosio 47,90 (V) i 21,42 (N). Nisu
ustanovljene statistički značajne (p>0,05) razlike u prosečom sadržaju proteina u spermalnoj
plazmi, između ispitivanih rasa nerastova. Ova vrednost je iznosila 2,43 procenta
kod rase landras, 2,45 kod rase veliki jorkšir i 1,83 kod rase durok. Prosečna vrednost
sadržaja proteina u spermalnoj plazmi nije značajno (p>0,05) varirala ni u zavisnosti od starosti ispitivanih nerastova. Navedene činjenice, ukazuju da sadržaj proteina u
spermalnoj plazmi, može biti efikasan marker fertilizacionog potencijala nerastova, na
osnovu koga se oni mogu selekcionisati za upotrebu u veštačkom osemenjavanju.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "32. Savetovanje veterinara Srbije, Zlatibor, 9 – 12. septembar 2021",
title = "Proteini spermalne plazme nerastova kao genetski markeri kvaliteta semena",
pages = "92-103",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3160"
}

Apić, J., Galić, I., Stančić, I., Barna, T., Vakanjac, S.,& Milovanović, A.. (2021). Proteini spermalne plazme nerastova kao genetski markeri kvaliteta semena. in 32. Savetovanje veterinara Srbije, Zlatibor, 9 – 12. septembar 2021
Beograd : Srpsko veterinarsko društvo., 92-103.
https://hdl.handle.net/21.15107/rcub_veterinar_3160

Apić J, Galić I, Stančić I, Barna T, Vakanjac S, Milovanović A. Proteini spermalne plazme nerastova kao genetski markeri kvaliteta semena. in 32. Savetovanje veterinara Srbije, Zlatibor, 9 – 12. septembar 2021. 2021;:92-103.
https://hdl.handle.net/21.15107/rcub_veterinar_3160 .

Apić, Jelena, Galić, Ivan, Stančić, Ivan, Barna, Tomislav, Vakanjac, Slobodanka, Milovanović, Aleksandar, "Proteini spermalne plazme nerastova kao genetski markeri kvaliteta semena" in 32. Savetovanje veterinara Srbije, Zlatibor, 9 – 12. septembar 2021 (2021):92-103,
https://hdl.handle.net/21.15107/rcub_veterinar_3160 .

TY  - JOUR
AU  - Barna, Tomislav
AU  - Apić, Jelena
AU  - Milovanović, Aleksandar
AU  - Maksimović, Nevena
AU  - Mašić, Aleksandar
AU  - Lazarević, Mmarina
AU  - Pavlović, Miloš
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2247
AB  - The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA), cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ± 100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%) in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters, as well as ratio of live sperm cells population with damaged acrosome. © 2021 TÜBİTAK.
PB  - Scientific Technical Research Council Turkey-Tubitak, Ankara
T2  - Turkish Journal of Veterinary and Animal Sciences
T1  - Quality and fertility of extended boar semen after prolonged transport
VL  - 45
IS  - 5
SP  - 920
EP  - 929
DO  - 10.3906/VET-2012-63
ER  - 

@article{
author = "Barna, Tomislav and Apić, Jelena and Milovanović, Aleksandar and Maksimović, Nevena and Mašić, Aleksandar and Lazarević, Mmarina and Pavlović, Miloš",
year = "2021",
abstract = "The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA), cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ± 100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%) in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters, as well as ratio of live sperm cells population with damaged acrosome. © 2021 TÜBİTAK.",
publisher = "Scientific Technical Research Council Turkey-Tubitak, Ankara",
journal = "Turkish Journal of Veterinary and Animal Sciences",
title = "Quality and fertility of extended boar semen after prolonged transport",
volume = "45",
number = "5",
pages = "920-929",
doi = "10.3906/VET-2012-63"
}

Barna, T., Apić, J., Milovanović, A., Maksimović, N., Mašić, A., Lazarević, M.,& Pavlović, M.. (2021). Quality and fertility of extended boar semen after prolonged transport. in Turkish Journal of Veterinary and Animal Sciences
Scientific Technical Research Council Turkey-Tubitak, Ankara., 45(5), 920-929.
https://doi.org/10.3906/VET-2012-63

Barna T, Apić J, Milovanović A, Maksimović N, Mašić A, Lazarević M, Pavlović M. Quality and fertility of extended boar semen after prolonged transport. in Turkish Journal of Veterinary and Animal Sciences. 2021;45(5):920-929.
doi:10.3906/VET-2012-63 .

Barna, Tomislav, Apić, Jelena, Milovanović, Aleksandar, Maksimović, Nevena, Mašić, Aleksandar, Lazarević, Mmarina, Pavlović, Miloš, "Quality and fertility of extended boar semen after prolonged transport" in Turkish Journal of Veterinary and Animal Sciences, 45, no. 5 (2021):920-929,
https://doi.org/10.3906/VET-2012-63 . .

TY  - CONF
AU  - Milovanoviić, Aleksandar
AU  - Barna, Tomislav
AU  - Apić, Jelena
AU  - Stojanov, Igor
AU  - Lazarević, Miodrag
AU  - Maksimović, Nevena
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3426
AB  - U ovom izlaganju će biti prikazan uticaj zračenja semena nerastova crvenim delom
svetlosnog spektra neposredno pre osemenjavanja na veličinu legla krmača. Korišćene su LED
lampe ugrađene u klima boks koje emituju spektar crvene boje, talasne dužine od 620 do 630
nm, sa režimom rada od 10 minuta svetlosti, 10 minuta odmora i 10 minuta dodatnog zračenja.
Osemenjavanja su sprovedena u periodu od aprila do maja 2021. godine, na farmi od 1
200 priplodnih krmača. U kontrolnoj grupi su bile 174 krmače, sa prosečnih 4,36 prašenja, a u
oglednoj 64, sa paritetom od 4,14. Nazimice nisu bile uključene u statističku obradu.
U oglednoj grupi je registrovan veći broj živorođene prasadi za 0,87 (14,05 prema
13,17), ali bez statističke značajnosti, dok je u istoj grupi, zabeležen znatno manji broj avitalne
i mrtvorođene prasadi (3,55 prema 4,68; p<0,05). Broj mumificirane prasadi u leglima je bio
identičan (0,45).
Zračenje semena crvenim svetlosnim zracima, neposredno pre osemenjavanja imalo je
pozitivan efekat na broj živorođene prasadi u leglu, ali samo u numeričkoj vrednosti, dok je
jasna statistička razlika uočena u pogledu njihove vitalnosti.
Ovaj ogled je u toku i očekuje se analiza znatno većeg broja prašenja, a time i jasniji
uvid u efekte ovakvog tretmana semena na oprasivost.
AB  - In this presentation, the influence of irradiation of boar semen by red light spectrum
immediately before insemination on the litter size in sows will be presented. LED lamps that
emit a red color spectrum with a wavelength of 620-630 nm built into the air conditioning box
were used. A mode of operation was: 10 minutes of light, 10 minutes of rest and 10 minutes of
additional irradiation.
Inseminations were carried out in the period from April to May 2021, on a farm of 1,200
breeding sows. Control group consisted of 174 sows with an average of 4.36 parities, and
experimental group of 64 sows, with a parity of 4.14. Gillts were excluded from the statistics.
In the experimental group, a higher number of live-born piglets was observed by 0.87
(14.05 vs. 13.17) but with no statistical significance, while in the same group, a significantly
lower number of avital and stillborn piglets was also observed (3.55 vs. 4.68; p <0.05 ). The
number of mummified piglets in the litters was identical (0.45 piglets) for both groups.
Semen irradiation with red light rays immediately before insemination had a positive
effect on the number of live-born piglets in the litter, but only in numerical value, while a clear
statistical difference was observed in terms of vitality of piglets in litters.
This trial is in progress and a significantly larger number of farrowings and a clearer
insight into the efficiency of such semen treatment on the fertility is expected.
PB  - Požarevac : Sitograf RM : Veterinarski specijalistički institut
C3  - 18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021
T1  - Uticaj zračenja semena nerastova crvenim delom svetlosnog spektra na veličinu legla krmača
T1  - Influence of red light spectrum irradiation of boar semen on litter size in sows
SP  - 71
EP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3426
ER  - 

@conference{
author = "Milovanoviić, Aleksandar and Barna, Tomislav and Apić, Jelena and Stojanov, Igor and Lazarević, Miodrag and Maksimović, Nevena",
year = "2021",
abstract = "U ovom izlaganju će biti prikazan uticaj zračenja semena nerastova crvenim delom
svetlosnog spektra neposredno pre osemenjavanja na veličinu legla krmača. Korišćene su LED
lampe ugrađene u klima boks koje emituju spektar crvene boje, talasne dužine od 620 do 630
nm, sa režimom rada od 10 minuta svetlosti, 10 minuta odmora i 10 minuta dodatnog zračenja.
Osemenjavanja su sprovedena u periodu od aprila do maja 2021. godine, na farmi od 1
200 priplodnih krmača. U kontrolnoj grupi su bile 174 krmače, sa prosečnih 4,36 prašenja, a u
oglednoj 64, sa paritetom od 4,14. Nazimice nisu bile uključene u statističku obradu.
U oglednoj grupi je registrovan veći broj živorođene prasadi za 0,87 (14,05 prema
13,17), ali bez statističke značajnosti, dok je u istoj grupi, zabeležen znatno manji broj avitalne
i mrtvorođene prasadi (3,55 prema 4,68; p<0,05). Broj mumificirane prasadi u leglima je bio
identičan (0,45).
Zračenje semena crvenim svetlosnim zracima, neposredno pre osemenjavanja imalo je
pozitivan efekat na broj živorođene prasadi u leglu, ali samo u numeričkoj vrednosti, dok je
jasna statistička razlika uočena u pogledu njihove vitalnosti.
Ovaj ogled je u toku i očekuje se analiza znatno većeg broja prašenja, a time i jasniji
uvid u efekte ovakvog tretmana semena na oprasivost., In this presentation, the influence of irradiation of boar semen by red light spectrum
immediately before insemination on the litter size in sows will be presented. LED lamps that
emit a red color spectrum with a wavelength of 620-630 nm built into the air conditioning box
were used. A mode of operation was: 10 minutes of light, 10 minutes of rest and 10 minutes of
additional irradiation.
Inseminations were carried out in the period from April to May 2021, on a farm of 1,200
breeding sows. Control group consisted of 174 sows with an average of 4.36 parities, and
experimental group of 64 sows, with a parity of 4.14. Gillts were excluded from the statistics.
In the experimental group, a higher number of live-born piglets was observed by 0.87
(14.05 vs. 13.17) but with no statistical significance, while in the same group, a significantly
lower number of avital and stillborn piglets was also observed (3.55 vs. 4.68; p <0.05 ). The
number of mummified piglets in the litters was identical (0.45 piglets) for both groups.
Semen irradiation with red light rays immediately before insemination had a positive
effect on the number of live-born piglets in the litter, but only in numerical value, while a clear
statistical difference was observed in terms of vitality of piglets in litters.
This trial is in progress and a significantly larger number of farrowings and a clearer
insight into the efficiency of such semen treatment on the fertility is expected.",
publisher = "Požarevac : Sitograf RM : Veterinarski specijalistički institut",
journal = "18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021",
title = "Uticaj zračenja semena nerastova crvenim delom svetlosnog spektra na veličinu legla krmača, Influence of red light spectrum irradiation of boar semen on litter size in sows",
pages = "71-72",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3426"
}

Milovanoviić, A., Barna, T., Apić, J., Stojanov, I., Lazarević, M.,& Maksimović, N.. (2021). Uticaj zračenja semena nerastova crvenim delom svetlosnog spektra na veličinu legla krmača. in 18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021
Požarevac : Sitograf RM : Veterinarski specijalistički institut., 71-72.
https://hdl.handle.net/21.15107/rcub_veterinar_3426

Milovanoviić A, Barna T, Apić J, Stojanov I, Lazarević M, Maksimović N. Uticaj zračenja semena nerastova crvenim delom svetlosnog spektra na veličinu legla krmača. in 18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021. 2021;:71-72.
https://hdl.handle.net/21.15107/rcub_veterinar_3426 .

Milovanoviić, Aleksandar, Barna, Tomislav, Apić, Jelena, Stojanov, Igor, Lazarević, Miodrag, Maksimović, Nevena, "Uticaj zračenja semena nerastova crvenim delom svetlosnog spektra na veličinu legla krmača" in 18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021 (2021):71-72,
https://hdl.handle.net/21.15107/rcub_veterinar_3426 .

TY  - JOUR
AU  - Stojanov, Igor
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Prodanov-Radulović, Jasna
AU  - Apić, Jelena
AU  - Stojanović, Dragica
AU  - Maksimović, Nevena
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2385
AB  - The aim of the study was to determine whether the bacteria from the environment and from the mucous membrane of the boar prepuce have antimicrobial resistance and whether the result obtained is similar/same to the bacteria that can be found in native boar semen. The study addresses the problem of the presence of primarily resistant bacterial strains in the boar sperm, which, due to their reduced sensitivity, cannot be suppressed by antibiotics used in the semen dilution agent, as well as to emphasize the importance of microbiological monitoring of the boar mucous membranes and ambient surfaces before and during their exploitation. Such an examination could contribute to the interchangeable design of the dilution agent for the boar semen relative to the antibiotic content.Resistant strains of bacteria from prepuce swabs and swabs taken from the facility, as well as from native boar semen were isolated. The presence of these bacteria affected the quality of the semen. In conclusion, it should be pointed out that bacterial monitoring of the prepuce and surface of the facility can indicate possible problems related to the quality of semen, and that the design of the dilution agent for boar semen should be adjusted to the established resistance of isolated bacteria.
AB  - Cilj ispitivanja je da se utvrdi da li bakterije iz okoline i sa sluznici prepucijma poseduju antimikrobnu rezistenciju i da li je dobijeni nalaz sličan/isti sa bakterijama koje se mogu naći u nativnom semenu nerasta. Ispitivanje treba da odgovori na problem prisustva, pre svega rezistentnih bakterijskih sojeva u spermi nerasta koji zbog svoje smanjene osteljivosti ne mogu biti suprimirani antibioticima, koji se nalaze u razređivaču za seme, kao i da istakne značaj mikrobiološkog monitoringa sluznice nerasta i ambijentalnih površina pre i tokom njihove eksploatacije. Ovakvo ispitivanje bi moglo da doprinese izmenjivom dizajniranju razređivača za seme nerasta u odnosu na sadržaj antibiotika. Izolovani su rezistentni sojevi bakterija iz briseva prepucijuma i briseva uzetih iz objekta kao i iz nativnog semena nerasta. Prisustvo ovih bakterija uticalo na kvalitet semena. Kao zaključak treba istaći da bakterijski monitoring prepucijuma i površina u objektu može ukazati na moguće probleme vezane za kvalitet semena nerasta kao i da se dizajniranje razređivača za seme nerasta prilagodi utvrđenoj rezistenciji izolovanih bakterija.
PB  - Faculty of Veterinary Medicine
PB  - Sciendo
T2  - Acta Veterinaria-Beograd
T1  - Antimicrobial resistance as a problem for the quality of Boar Semen
T1  - Antimikrobna rezistencija kao problem kvaliteta semena nerasta
VL  - 70
IS  - 1
SP  - 136
EP  - 146
DO  - 10.2478/acve-2020-0010
ER  - 

@article{
author = "Stojanov, Igor and Milovanović, Aleksandar and Barna, Tomislav and Prodanov-Radulović, Jasna and Apić, Jelena and Stojanović, Dragica and Maksimović, Nevena",
year = "2020",
abstract = "The aim of the study was to determine whether the bacteria from the environment and from the mucous membrane of the boar prepuce have antimicrobial resistance and whether the result obtained is similar/same to the bacteria that can be found in native boar semen. The study addresses the problem of the presence of primarily resistant bacterial strains in the boar sperm, which, due to their reduced sensitivity, cannot be suppressed by antibiotics used in the semen dilution agent, as well as to emphasize the importance of microbiological monitoring of the boar mucous membranes and ambient surfaces before and during their exploitation. Such an examination could contribute to the interchangeable design of the dilution agent for the boar semen relative to the antibiotic content.Resistant strains of bacteria from prepuce swabs and swabs taken from the facility, as well as from native boar semen were isolated. The presence of these bacteria affected the quality of the semen. In conclusion, it should be pointed out that bacterial monitoring of the prepuce and surface of the facility can indicate possible problems related to the quality of semen, and that the design of the dilution agent for boar semen should be adjusted to the established resistance of isolated bacteria., Cilj ispitivanja je da se utvrdi da li bakterije iz okoline i sa sluznici prepucijma poseduju antimikrobnu rezistenciju i da li je dobijeni nalaz sličan/isti sa bakterijama koje se mogu naći u nativnom semenu nerasta. Ispitivanje treba da odgovori na problem prisustva, pre svega rezistentnih bakterijskih sojeva u spermi nerasta koji zbog svoje smanjene osteljivosti ne mogu biti suprimirani antibioticima, koji se nalaze u razređivaču za seme, kao i da istakne značaj mikrobiološkog monitoringa sluznice nerasta i ambijentalnih površina pre i tokom njihove eksploatacije. Ovakvo ispitivanje bi moglo da doprinese izmenjivom dizajniranju razređivača za seme nerasta u odnosu na sadržaj antibiotika. Izolovani su rezistentni sojevi bakterija iz briseva prepucijuma i briseva uzetih iz objekta kao i iz nativnog semena nerasta. Prisustvo ovih bakterija uticalo na kvalitet semena. Kao zaključak treba istaći da bakterijski monitoring prepucijuma i površina u objektu može ukazati na moguće probleme vezane za kvalitet semena nerasta kao i da se dizajniranje razređivača za seme nerasta prilagodi utvrđenoj rezistenciji izolovanih bakterija.",
publisher = "Faculty of Veterinary Medicine, Sciendo",
journal = "Acta Veterinaria-Beograd",
title = "Antimicrobial resistance as a problem for the quality of Boar Semen, Antimikrobna rezistencija kao problem kvaliteta semena nerasta",
volume = "70",
number = "1",
pages = "136-146",
doi = "10.2478/acve-2020-0010"
}

Stojanov, I., Milovanović, A., Barna, T., Prodanov-Radulović, J., Apić, J., Stojanović, D.,& Maksimović, N.. (2020). Antimicrobial resistance as a problem for the quality of Boar Semen. in Acta Veterinaria-Beograd
Faculty of Veterinary Medicine., 70(1), 136-146.
https://doi.org/10.2478/acve-2020-0010

Stojanov I, Milovanović A, Barna T, Prodanov-Radulović J, Apić J, Stojanović D, Maksimović N. Antimicrobial resistance as a problem for the quality of Boar Semen. in Acta Veterinaria-Beograd. 2020;70(1):136-146.
doi:10.2478/acve-2020-0010 .

Stojanov, Igor, Milovanović, Aleksandar, Barna, Tomislav, Prodanov-Radulović, Jasna, Apić, Jelena, Stojanović, Dragica, Maksimović, Nevena, "Antimicrobial resistance as a problem for the quality of Boar Semen" in Acta Veterinaria-Beograd, 70, no. 1 (2020):136-146,
https://doi.org/10.2478/acve-2020-0010 . .

TY  - CONF
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Apić, Jelena
AU  - Stojanov, Igor
AU  - Lazarević, Miodrag
AU  - Vasiljević, Teodora
AU  - Antić, Danko
AU  - Mašić, Aleksandar
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3435
PB  - Požarevac : Veterinarski specijalistički institut
C3  - 17. Savetovanje Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019
T1  - Primena imunostimulatora u korekciji plodnosti krmača i odgoju prasadi u ranoj fazi - preliminarni rezultati
SP  - 142
EP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3435
ER  - 

@conference{
author = "Milovanović, Aleksandar and Barna, Tomislav and Apić, Jelena and Stojanov, Igor and Lazarević, Miodrag and Vasiljević, Teodora and Antić, Danko and Mašić, Aleksandar",
year = "2019",
publisher = "Požarevac : Veterinarski specijalistički institut",
journal = "17. Savetovanje Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019",
title = "Primena imunostimulatora u korekciji plodnosti krmača i odgoju prasadi u ranoj fazi - preliminarni rezultati",
pages = "142-143",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3435"
}

Milovanović, A., Barna, T., Apić, J., Stojanov, I., Lazarević, M., Vasiljević, T., Antić, D.,& Mašić, A.. (2019). Primena imunostimulatora u korekciji plodnosti krmača i odgoju prasadi u ranoj fazi - preliminarni rezultati. in 17. Savetovanje Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019
Požarevac : Veterinarski specijalistički institut., 142-143.
https://hdl.handle.net/21.15107/rcub_veterinar_3435

Milovanović A, Barna T, Apić J, Stojanov I, Lazarević M, Vasiljević T, Antić D, Mašić A. Primena imunostimulatora u korekciji plodnosti krmača i odgoju prasadi u ranoj fazi - preliminarni rezultati. in 17. Savetovanje Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019. 2019;:142-143.
https://hdl.handle.net/21.15107/rcub_veterinar_3435 .

Milovanović, Aleksandar, Barna, Tomislav, Apić, Jelena, Stojanov, Igor, Lazarević, Miodrag, Vasiljević, Teodora, Antić, Danko, Mašić, Aleksandar, "Primena imunostimulatora u korekciji plodnosti krmača i odgoju prasadi u ranoj fazi - preliminarni rezultati" in 17. Savetovanje Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019 (2019):142-143,
https://hdl.handle.net/21.15107/rcub_veterinar_3435 .

TY  - JOUR
AU  - Stančić, Ivan
AU  - Zdraveski, Igor
AU  - Dragin, Sasa
AU  - Apić, Jelena
AU  - Vakanjac, Slobodanka
AU  - Dodovski, Petar
AU  - Krstović, Saša
AU  - Galić, Ivan
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1698
AB  - The aim of this study was to investigate the effect of different percentage of seminal plasma proteins with different molecular weight on sperm motility and fertility parameters (farrowing rate (FR), number of live-born pigs (PBA) per litter and percentage of unsuccessful insemination). A total of 50 sperm-rich ejaculate fractions were collected (one per boar) using the gloved hand method. The quality parameters of the semen samples were first evaluated at the farm. Further assessment of sperm quality was performed on a CASA - computer assisted semen analysis by two competent operators. Seminal plasma protein fractions were obtained by AOAC -Association of Official Analytical Chemists as a chemical method. The assessment of reproductive performance was carried out based on collected data of three parameters in selected 9696 sows: FR, PBA per litter and percentage of unsuccessful insemination. Protein fractions were divided in to three groups (10 - 20kDa, 21 - 30kDa and 31-40kDa) Proteins with 10 - 20kDa did not have significant effect and correlation with analyzed parameters. Significant differences were recorded in farrowing rate between samples with up to 80 % compared to samples with 10% of proteins with 21 - 30kDa. Significant differences were recorded in unsuccessful insemination between samples with different percentage of proteins with 31 - 40kDa. Results of this study have shown the effect of different percentage of certain fraction of seminal plasma proteins on boar ejaculates fertility potential.
PB  - Univ Ljubljana, Veterinary Faculty, Ljubljana
T2  - Slovenian Veterinary Research
T1  - The influence of different molecular weight seminal plasma protein content on some fertility parameters in boar s ejaculates
VL  - 56
IS  - 4
SP  - 163
EP  - 171
DO  - 10.26873/SVR-738-2019
ER  - 

@article{
author = "Stančić, Ivan and Zdraveski, Igor and Dragin, Sasa and Apić, Jelena and Vakanjac, Slobodanka and Dodovski, Petar and Krstović, Saša and Galić, Ivan",
year = "2019",
abstract = "The aim of this study was to investigate the effect of different percentage of seminal plasma proteins with different molecular weight on sperm motility and fertility parameters (farrowing rate (FR), number of live-born pigs (PBA) per litter and percentage of unsuccessful insemination). A total of 50 sperm-rich ejaculate fractions were collected (one per boar) using the gloved hand method. The quality parameters of the semen samples were first evaluated at the farm. Further assessment of sperm quality was performed on a CASA - computer assisted semen analysis by two competent operators. Seminal plasma protein fractions were obtained by AOAC -Association of Official Analytical Chemists as a chemical method. The assessment of reproductive performance was carried out based on collected data of three parameters in selected 9696 sows: FR, PBA per litter and percentage of unsuccessful insemination. Protein fractions were divided in to three groups (10 - 20kDa, 21 - 30kDa and 31-40kDa) Proteins with 10 - 20kDa did not have significant effect and correlation with analyzed parameters. Significant differences were recorded in farrowing rate between samples with up to 80 % compared to samples with 10% of proteins with 21 - 30kDa. Significant differences were recorded in unsuccessful insemination between samples with different percentage of proteins with 31 - 40kDa. Results of this study have shown the effect of different percentage of certain fraction of seminal plasma proteins on boar ejaculates fertility potential.",
publisher = "Univ Ljubljana, Veterinary Faculty, Ljubljana",
journal = "Slovenian Veterinary Research",
title = "The influence of different molecular weight seminal plasma protein content on some fertility parameters in boar s ejaculates",
volume = "56",
number = "4",
pages = "163-171",
doi = "10.26873/SVR-738-2019"
}

Stančić, I., Zdraveski, I., Dragin, S., Apić, J., Vakanjac, S., Dodovski, P., Krstović, S.,& Galić, I.. (2019). The influence of different molecular weight seminal plasma protein content on some fertility parameters in boar s ejaculates. in Slovenian Veterinary Research
Univ Ljubljana, Veterinary Faculty, Ljubljana., 56(4), 163-171.
https://doi.org/10.26873/SVR-738-2019

Stančić I, Zdraveski I, Dragin S, Apić J, Vakanjac S, Dodovski P, Krstović S, Galić I. The influence of different molecular weight seminal plasma protein content on some fertility parameters in boar s ejaculates. in Slovenian Veterinary Research. 2019;56(4):163-171.
doi:10.26873/SVR-738-2019 .

Stančić, Ivan, Zdraveski, Igor, Dragin, Sasa, Apić, Jelena, Vakanjac, Slobodanka, Dodovski, Petar, Krstović, Saša, Galić, Ivan, "The influence of different molecular weight seminal plasma protein content on some fertility parameters in boar s ejaculates" in Slovenian Veterinary Research, 56, no. 4 (2019):163-171,
https://doi.org/10.26873/SVR-738-2019 . .

TY  - JOUR
AU  - Vakanjac, Slobodanka
AU  - Magaš, Vladimir
AU  - Babić, Vladan
AU  - Maletić, Milan
AU  - Đurić, Spomenka
AU  - Apić, Jelena
AU  - Mirilović, Milorad
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1444
AB  - Introduction. Artificial insemination of sows with cooled semen has become a routine practice in the swine industry and has almost completely ruled out natural mating. The storage time of the cooled semen and preservation of its qualitative parameters are of the upmost importance and depend partly on the semen extender used. The aim of this study was to determine the quality of cooled semen during storage in a thermobox with a commercial extender by assessment of sperm motility, cytomorphology, and assessment of reproductive parameters in sows after intrauterine insemination. Materials and Methods. The semen was preserved with Duragen® (Magapor, Spain) extender and stored at 17±1°C. A total of 110 sows were included in the study and randomly divided into four groups: K1 (n=25) , K2 (n=25), K3 (n=30), and K4 (n=30). The sows were inseminated twice with semen (>1x107/ml spermatozoa) which had been stored for 1 day (S1), 3 days (S3), 5 days (S5), or 7 days (S7). Pregnancy was diagnosed by imaging ultrasound. Results and Conclusions. The best results were obtained when the sows were inseminated with semen stored for one day. However, there were no statistically significant differences in the number and vitality of newborn piglets when semen stored for 3 or 5 days was used. Intrauterine insemination resulted in a satisfactory number of farrowing's and piglets despite the fact that a relatively low number of spermatozoa was determined in each dose used. The use of semen with a higher dilution rate and its intrauterine application ensured a larger number of obtained doses from one ejaculate and indicates this would lead to a more profitable use of boars.
AB  - Uvod. Veštačko osemenjavanje svinja rashlađenim semenom je postala rutinska praksa u industrijskoj proizvodnji svinja, pa je u razvijenim zemljama skoro potpuno zamenilo prirodno parenje. U zavisnosti od vrste upotrebljenog razređivača, dužina čuvanja rashlađenog semena i očuvanje njegovih kvalitativnih parametara je od izuzetne važnosti. Cilj istraživanja je analiza kvaliteta rashlađenog semena tokom njegovog skladištenja u termoboksu pri 17±1°C. Materijal i metode. Seme je konzervirano upotrebom razređivača Duragen® (Magapor, Španija) i skladišteno pri temperaturi od 17±1°C. U ogled je uključeno 110 krmača, podeljenih u četiri grupe (K1, K3, K5, K7). Krmače su osemenjivane dvokratno semenom skladištenim 1 dan (S1), 3 dana (S3), 5 dana (S5) i 7 dana (S7). Ultrazvučnim pregledom je vršena dijagnostika graviditeta. Kvalitet semena je ocenjivan ispitivanjem pokretljivosti spermatozoida i citomorfološkim pregledom i praćenjem reproduktivnih parametara kod intrauterino osemenjenih krmača. Rezultati i zaključak. Najbolji rezultat je postignut osemenjavanjem krmača semenom starim jedan dan. Međutim, nije bilo značajne razlike u broju i vitalnosti oprašene prasadi korišćenjem rashlađenog semena starog 3 i 5 dana. Intrauterinim osemenjavanjem su dobijeni zadovoljavajući rezultati (procenat prašenja i veličina legla) sa manjim brojem spermatozoida u inseminacionoj dozi. Upotrebom semena sa većim razređenjem i njegovim intrauterinim deponovanjem, omogućeno je dobijanje većeg broja inseminacionih doza semena od jednog ejakulata i rentabilnije korišćenje nerastova u reprodukciji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Analysis of reproductive parameters after intrauterine insemination of sows with semen stored for different time periods
T1  - Analiza reproduktivnih parametara intrauterino osemenjenih krmača u zavisnosti od vremena čuvanja razređenog semena
VL  - 71
IS  - 2
SP  - 98
EP  - 106
DO  - 10.2298/VETGL1702098V
ER  - 

@article{
author = "Vakanjac, Slobodanka and Magaš, Vladimir and Babić, Vladan and Maletić, Milan and Đurić, Spomenka and Apić, Jelena and Mirilović, Milorad",
year = "2017",
abstract = "Introduction. Artificial insemination of sows with cooled semen has become a routine practice in the swine industry and has almost completely ruled out natural mating. The storage time of the cooled semen and preservation of its qualitative parameters are of the upmost importance and depend partly on the semen extender used. The aim of this study was to determine the quality of cooled semen during storage in a thermobox with a commercial extender by assessment of sperm motility, cytomorphology, and assessment of reproductive parameters in sows after intrauterine insemination. Materials and Methods. The semen was preserved with Duragen® (Magapor, Spain) extender and stored at 17±1°C. A total of 110 sows were included in the study and randomly divided into four groups: K1 (n=25) , K2 (n=25), K3 (n=30), and K4 (n=30). The sows were inseminated twice with semen (>1x107/ml spermatozoa) which had been stored for 1 day (S1), 3 days (S3), 5 days (S5), or 7 days (S7). Pregnancy was diagnosed by imaging ultrasound. Results and Conclusions. The best results were obtained when the sows were inseminated with semen stored for one day. However, there were no statistically significant differences in the number and vitality of newborn piglets when semen stored for 3 or 5 days was used. Intrauterine insemination resulted in a satisfactory number of farrowing's and piglets despite the fact that a relatively low number of spermatozoa was determined in each dose used. The use of semen with a higher dilution rate and its intrauterine application ensured a larger number of obtained doses from one ejaculate and indicates this would lead to a more profitable use of boars., Uvod. Veštačko osemenjavanje svinja rashlađenim semenom je postala rutinska praksa u industrijskoj proizvodnji svinja, pa je u razvijenim zemljama skoro potpuno zamenilo prirodno parenje. U zavisnosti od vrste upotrebljenog razređivača, dužina čuvanja rashlađenog semena i očuvanje njegovih kvalitativnih parametara je od izuzetne važnosti. Cilj istraživanja je analiza kvaliteta rashlađenog semena tokom njegovog skladištenja u termoboksu pri 17±1°C. Materijal i metode. Seme je konzervirano upotrebom razređivača Duragen® (Magapor, Španija) i skladišteno pri temperaturi od 17±1°C. U ogled je uključeno 110 krmača, podeljenih u četiri grupe (K1, K3, K5, K7). Krmače su osemenjivane dvokratno semenom skladištenim 1 dan (S1), 3 dana (S3), 5 dana (S5) i 7 dana (S7). Ultrazvučnim pregledom je vršena dijagnostika graviditeta. Kvalitet semena je ocenjivan ispitivanjem pokretljivosti spermatozoida i citomorfološkim pregledom i praćenjem reproduktivnih parametara kod intrauterino osemenjenih krmača. Rezultati i zaključak. Najbolji rezultat je postignut osemenjavanjem krmača semenom starim jedan dan. Međutim, nije bilo značajne razlike u broju i vitalnosti oprašene prasadi korišćenjem rashlađenog semena starog 3 i 5 dana. Intrauterinim osemenjavanjem su dobijeni zadovoljavajući rezultati (procenat prašenja i veličina legla) sa manjim brojem spermatozoida u inseminacionoj dozi. Upotrebom semena sa većim razređenjem i njegovim intrauterinim deponovanjem, omogućeno je dobijanje većeg broja inseminacionih doza semena od jednog ejakulata i rentabilnije korišćenje nerastova u reprodukciji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Analysis of reproductive parameters after intrauterine insemination of sows with semen stored for different time periods, Analiza reproduktivnih parametara intrauterino osemenjenih krmača u zavisnosti od vremena čuvanja razređenog semena",
volume = "71",
number = "2",
pages = "98-106",
doi = "10.2298/VETGL1702098V"
}

Vakanjac, S., Magaš, V., Babić, V., Maletić, M., Đurić, S., Apić, J.,& Mirilović, M.. (2017). Analysis of reproductive parameters after intrauterine insemination of sows with semen stored for different time periods. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(2), 98-106.
https://doi.org/10.2298/VETGL1702098V

Vakanjac S, Magaš V, Babić V, Maletić M, Đurić S, Apić J, Mirilović M. Analysis of reproductive parameters after intrauterine insemination of sows with semen stored for different time periods. in Veterinarski Glasnik. 2017;71(2):98-106.
doi:10.2298/VETGL1702098V .

Vakanjac, Slobodanka, Magaš, Vladimir, Babić, Vladan, Maletić, Milan, Đurić, Spomenka, Apić, Jelena, Mirilović, Milorad, "Analysis of reproductive parameters after intrauterine insemination of sows with semen stored for different time periods" in Veterinarski Glasnik, 71, no. 2 (2017):98-106,
https://doi.org/10.2298/VETGL1702098V . .

TY  - JOUR
AU  - Apić, Jelena
AU  - Stančić, Ivan
AU  - Vakanjac, Slobodanka
AU  - Radović, I.
AU  - Milovanović, A.
AU  - Barna, Tomislav
AU  - Maletić, Milan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1333
AB  - The aim of the present study was to investigate the influence of the protein content of seminal plasma on the motility, viability and acrosome integrity of spermatozoa in extended semen stored for 3 days. A total of 32 semen samples (from four boars) with high (4 mg/ml) and 32 semen samples (from four boars) with low (2 mg/ml) protein content were investigated. The semen samples were diluted by BTS at a ratio of 1:4, and stored for 72 h at 17oC. The percentages of live sperm (LS), live sperm with damaged acrosome (LDA) and total sperm with damaged acrosome (TDA) were detected by flow cytometry. Sperm progressive motility (PM) was detected using CASA. After 72 h of storage, the percentage of LS and PM was significantly (P  lt  0.01) higher, and the LDA and TDA were significantly (P  lt  0.01) lower in samples with high protein content than in the samples with low protein content (LS = 66 vs. 44%, PM = 64 vs. 48%, LDA = 15 vs. 21% and TDA = 29 vs. 45%, respectively). When comparing the difference between 0 and 72 h of storage, the percentage decrease in LS and PM, while increase in LDA and TDA were significantly higher in the samples with low (LS: 75 to 44%; PM: 68 to 48%; LDA: 11 to 21% and TDA: 23 to 45%) than in the samples with high protein content (LS: 78 to 66%; PM: 70 to 64%; LDA: 9 to 15% and TDA: 17 to 29%). We concluded that protein content in seminal plasma has a significant influence on progressive motility, viability and acrosome integrity in diluted semen stored for 3 days.
PB  - Colegio Brasileiro de Reproducao Animal
T2  - Animal Reproduction
T1  - Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days
VL  - 13
IS  - 1
SP  - 36
EP  - 41
DO  - 10.4322/1984-3143-AR792
ER  - 

@article{
author = "Apić, Jelena and Stančić, Ivan and Vakanjac, Slobodanka and Radović, I. and Milovanović, A. and Barna, Tomislav and Maletić, Milan",
year = "2016",
abstract = "The aim of the present study was to investigate the influence of the protein content of seminal plasma on the motility, viability and acrosome integrity of spermatozoa in extended semen stored for 3 days. A total of 32 semen samples (from four boars) with high (4 mg/ml) and 32 semen samples (from four boars) with low (2 mg/ml) protein content were investigated. The semen samples were diluted by BTS at a ratio of 1:4, and stored for 72 h at 17oC. The percentages of live sperm (LS), live sperm with damaged acrosome (LDA) and total sperm with damaged acrosome (TDA) were detected by flow cytometry. Sperm progressive motility (PM) was detected using CASA. After 72 h of storage, the percentage of LS and PM was significantly (P  lt  0.01) higher, and the LDA and TDA were significantly (P  lt  0.01) lower in samples with high protein content than in the samples with low protein content (LS = 66 vs. 44%, PM = 64 vs. 48%, LDA = 15 vs. 21% and TDA = 29 vs. 45%, respectively). When comparing the difference between 0 and 72 h of storage, the percentage decrease in LS and PM, while increase in LDA and TDA were significantly higher in the samples with low (LS: 75 to 44%; PM: 68 to 48%; LDA: 11 to 21% and TDA: 23 to 45%) than in the samples with high protein content (LS: 78 to 66%; PM: 70 to 64%; LDA: 9 to 15% and TDA: 17 to 29%). We concluded that protein content in seminal plasma has a significant influence on progressive motility, viability and acrosome integrity in diluted semen stored for 3 days.",
publisher = "Colegio Brasileiro de Reproducao Animal",
journal = "Animal Reproduction",
title = "Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days",
volume = "13",
number = "1",
pages = "36-41",
doi = "10.4322/1984-3143-AR792"
}

Apić, J., Stančić, I., Vakanjac, S., Radović, I., Milovanović, A., Barna, T.,& Maletić, M.. (2016). Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days. in Animal Reproduction
Colegio Brasileiro de Reproducao Animal., 13(1), 36-41.
https://doi.org/10.4322/1984-3143-AR792

Apić J, Stančić I, Vakanjac S, Radović I, Milovanović A, Barna T, Maletić M. Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days. in Animal Reproduction. 2016;13(1):36-41.
doi:10.4322/1984-3143-AR792 .

Apić, Jelena, Stančić, Ivan, Vakanjac, Slobodanka, Radović, I., Milovanović, A., Barna, Tomislav, Maletić, Milan, "Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days" in Animal Reproduction, 13, no. 1 (2016):36-41,
https://doi.org/10.4322/1984-3143-AR792 . .

TY  - JOUR
AU  - Apić, Jelena
AU  - Radović, Ivan
AU  - Stančić, Ivan
AU  - Vakanjac, Slobodanka
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1346
AB  - In order to determine the more accurate fertile potential of sperm, it seems that the conventional parameters of boar semen quality (the ejaculate volume, sperm concentration, progressive motility, percentage of live sperm and of those with intact acrosomal morphology) are insufficient. Since recently, there have been numerous studies proving that protein concentration in sperm plasma has high positive correlation with boar fertile potential. The research objective was to determine the effect of boars as well as the season on the variation of protein content in the sperm plasma. For the research there were used spermal fractions of 2 boars with high (V-boar) and 2 boars with low (N-boar) protein content in spermal plasma. The ejaculates of boars were taken once a week, for a month, during one year (4 *12 = 48 ejaculates per boar). For protein analysis in the spermal plasma, the samples were prepared by centrifugation. The ejaculate volume, protein concentration and progressive motility varied considerably (p  lt  0.05 or p  lt  0.01) among the boars as well as in one and the same boar. The variations of the same parameters were determined also during both warm and cold season. On the other hand, protein concentration was rather constant, and in V-boars (ranged from 4 to 4.5%) while in N-boars (ranged from 2.3 to 2.6%). The season did not significantly affect (p > 0.01) protein content in sperm plasma (V-boars: 4 to 4.5% in warm and cold season; N-boars: 2.3 do 2.6% in warm and 2.3 to 2.5% in cold season). The obtained results showed that measurement of protein content in boar sperm plasma could be a useful method for their ranking, based on fertile potential of fresh semen.
AB  - U cilju određivanja što tačnijeg fertilizacionog potencijala spermatozoida, čini se da konvencionalni parametri kvaliteta semena nerastova (volumen ejakulata, koncentracja spermatozoida, progresivna pokretljivost, procenat živih spermatozoida, kao i onih sa intaktnom akrozomalnom morfologijom) nisu dovoljni. Od nedavno, postoje brojna istraživanja koja dokazuju da koncentracja proteina u spermalnoj plazmi ima visoku pozitivnu korelaciju sa fertilizacionim potencijalom nerastova. Cilj ovog istraživanja bio je da se utvrdi efekt nerasta kao i uticaj godišnje sezone na varijaciju sadržaja proteina u spermalnoj plazmi nerastova. Za ispitivanja su korištene spermalne frakcje 2 nerasta sa visokim (V-nerast) i 2 narasta sa niskim (N-nerast) sadržajem proteina u spermalnoj plazmi. Ejakulati su od nerastova uzimani jednom nedeljno, u okviru jednog meseca, tokom godinu dana (4*12=48 ejakulata po nerastu). Za analizu proteina u spermalnoj plazmi, uzorci su pripremljeni centrifugiranjem. Volumen ejakulata, koncentacja spermatozoida kao i progresivna pokretljivost su značajno varirali (p  lt  0,05 ili p  lt  0,01) između nerastova kao i kod jednog te istog nerasta. Varjacje istih parametara utvrđene su i tokom tople odnosno hladne sezone godine. Sa druge strane, koncentracja proteina je bila prilično konstantna, i kod V-nerastova (kretala se od 4 do 4,5%) i kod N-nerastova (kretala se od 2,3 do 2,6%). Sezona nije značajno uticala (p > 0,01) na sadržaj proteina u spermalnoj plazmi (V-nerastovi: 4 do 4,5% u toploj i hladnoj sezoni godine; N-nerastovi: 2,3 do 2,6% u toploj i 2,3 do 2,5% u hladnoj sezoni godine). Dobijeni rezultati pokazuju da bi merenje sadržaja proteina u spermalnoj plazmi nerastova mogao biti koristan metod za njihovo rangiranje, a na osnovu fertilizacionog potencijala svežeg semena.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Boar and season effects on some parameters of semen fertilizing potential
T1  - Vlijanie hrjaka i vremeni goda na otdel'nye parametry fertilizacionnogo potenciala spermatozoidov
T1  - Uticaj nerasta i godišnje sezone na pojedine parametre fertilizacionog potencijala spermatozoida
VL  - 70
IS  - 5-6
SP  - 163
EP  - 174
DO  - 10.2298/VETGL1606163A
ER  - 

@article{
author = "Apić, Jelena and Radović, Ivan and Stančić, Ivan and Vakanjac, Slobodanka",
year = "2016",
abstract = "In order to determine the more accurate fertile potential of sperm, it seems that the conventional parameters of boar semen quality (the ejaculate volume, sperm concentration, progressive motility, percentage of live sperm and of those with intact acrosomal morphology) are insufficient. Since recently, there have been numerous studies proving that protein concentration in sperm plasma has high positive correlation with boar fertile potential. The research objective was to determine the effect of boars as well as the season on the variation of protein content in the sperm plasma. For the research there were used spermal fractions of 2 boars with high (V-boar) and 2 boars with low (N-boar) protein content in spermal plasma. The ejaculates of boars were taken once a week, for a month, during one year (4 *12 = 48 ejaculates per boar). For protein analysis in the spermal plasma, the samples were prepared by centrifugation. The ejaculate volume, protein concentration and progressive motility varied considerably (p  lt  0.05 or p  lt  0.01) among the boars as well as in one and the same boar. The variations of the same parameters were determined also during both warm and cold season. On the other hand, protein concentration was rather constant, and in V-boars (ranged from 4 to 4.5%) while in N-boars (ranged from 2.3 to 2.6%). The season did not significantly affect (p > 0.01) protein content in sperm plasma (V-boars: 4 to 4.5% in warm and cold season; N-boars: 2.3 do 2.6% in warm and 2.3 to 2.5% in cold season). The obtained results showed that measurement of protein content in boar sperm plasma could be a useful method for their ranking, based on fertile potential of fresh semen., U cilju određivanja što tačnijeg fertilizacionog potencijala spermatozoida, čini se da konvencionalni parametri kvaliteta semena nerastova (volumen ejakulata, koncentracja spermatozoida, progresivna pokretljivost, procenat živih spermatozoida, kao i onih sa intaktnom akrozomalnom morfologijom) nisu dovoljni. Od nedavno, postoje brojna istraživanja koja dokazuju da koncentracja proteina u spermalnoj plazmi ima visoku pozitivnu korelaciju sa fertilizacionim potencijalom nerastova. Cilj ovog istraživanja bio je da se utvrdi efekt nerasta kao i uticaj godišnje sezone na varijaciju sadržaja proteina u spermalnoj plazmi nerastova. Za ispitivanja su korištene spermalne frakcje 2 nerasta sa visokim (V-nerast) i 2 narasta sa niskim (N-nerast) sadržajem proteina u spermalnoj plazmi. Ejakulati su od nerastova uzimani jednom nedeljno, u okviru jednog meseca, tokom godinu dana (4*12=48 ejakulata po nerastu). Za analizu proteina u spermalnoj plazmi, uzorci su pripremljeni centrifugiranjem. Volumen ejakulata, koncentacja spermatozoida kao i progresivna pokretljivost su značajno varirali (p  lt  0,05 ili p  lt  0,01) između nerastova kao i kod jednog te istog nerasta. Varjacje istih parametara utvrđene su i tokom tople odnosno hladne sezone godine. Sa druge strane, koncentracja proteina je bila prilično konstantna, i kod V-nerastova (kretala se od 4 do 4,5%) i kod N-nerastova (kretala se od 2,3 do 2,6%). Sezona nije značajno uticala (p > 0,01) na sadržaj proteina u spermalnoj plazmi (V-nerastovi: 4 do 4,5% u toploj i hladnoj sezoni godine; N-nerastovi: 2,3 do 2,6% u toploj i 2,3 do 2,5% u hladnoj sezoni godine). Dobijeni rezultati pokazuju da bi merenje sadržaja proteina u spermalnoj plazmi nerastova mogao biti koristan metod za njihovo rangiranje, a na osnovu fertilizacionog potencijala svežeg semena.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Boar and season effects on some parameters of semen fertilizing potential, Vlijanie hrjaka i vremeni goda na otdel'nye parametry fertilizacionnogo potenciala spermatozoidov, Uticaj nerasta i godišnje sezone na pojedine parametre fertilizacionog potencijala spermatozoida",
volume = "70",
number = "5-6",
pages = "163-174",
doi = "10.2298/VETGL1606163A"
}

Apić, J., Radović, I., Stančić, I.,& Vakanjac, S.. (2016). Boar and season effects on some parameters of semen fertilizing potential. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(5-6), 163-174.
https://doi.org/10.2298/VETGL1606163A

Apić J, Radović I, Stančić I, Vakanjac S. Boar and season effects on some parameters of semen fertilizing potential. in Veterinarski Glasnik. 2016;70(5-6):163-174.
doi:10.2298/VETGL1606163A .

Apić, Jelena, Radović, Ivan, Stančić, Ivan, Vakanjac, Slobodanka, "Boar and season effects on some parameters of semen fertilizing potential" in Veterinarski Glasnik, 70, no. 5-6 (2016):163-174,
https://doi.org/10.2298/VETGL1606163A . .

TY  - JOUR
AU  - Apić, Jelena
AU  - Vakanjac, Slobodanka
AU  - Radović, Ivan
AU  - Jotanović, Stoja
AU  - Živkov-Baloš, Milica
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Stanković, Branislav
AU  - Jajić, Igor
AU  - Maletić, Milan
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1276
AB  - Recently, it was frequently demonstrated that fertility of sows after artificially inseminated is lower than after mating. This is associated with a reduced fertilization capacity of overdiluted insemination doses. The aim of this study was to investigate the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17 degrees C for 3 days. Progressive motility was significantly higher (p<0.01) in the ejaculates with high, compared to the ejaculates with low protein content (82% vs. 76%). After 3 days of storage, in the1:4 dilution proportion, the average progressive motility was significantly (p<0.01) decreased in relation to this value in native semen from the boars with high (82% to 64%), as well from the boars with low protein content in seminal plasma (76% to 48%). However, the average diluted semen progressive motility was significantly greater (p<0.01) in the boars with high (64%), compared to the boars with low protein content in seminal plasma (48%). The number of good diluted semen samples (>= 65% progressive motility), was also significantly (p<0.01) greater in the boars with high (41%), compared to the boars with low protein content in seminal plasma (12%). These results show that seminal plasma proteins play an important role in maintaining the sperm progressive motility of diluted semen in vitro stored for 3 days.
PB  - Slovak Univ Agriculture Nitra, Nitra
T2  - Journal of Microbiology Biotechnology and Food Sciences
T1  - Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days
VL  - 4
SP  - 1
EP  - 3
DO  - 10.15414/jmbfs.2015.4.special2.1-3
ER  - 

@article{
author = "Apić, Jelena and Vakanjac, Slobodanka and Radović, Ivan and Jotanović, Stoja and Živkov-Baloš, Milica and Milovanović, Aleksandar and Barna, Tomislav and Stanković, Branislav and Jajić, Igor and Maletić, Milan",
year = "2015",
abstract = "Recently, it was frequently demonstrated that fertility of sows after artificially inseminated is lower than after mating. This is associated with a reduced fertilization capacity of overdiluted insemination doses. The aim of this study was to investigate the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17 degrees C for 3 days. Progressive motility was significantly higher (p<0.01) in the ejaculates with high, compared to the ejaculates with low protein content (82% vs. 76%). After 3 days of storage, in the1:4 dilution proportion, the average progressive motility was significantly (p<0.01) decreased in relation to this value in native semen from the boars with high (82% to 64%), as well from the boars with low protein content in seminal plasma (76% to 48%). However, the average diluted semen progressive motility was significantly greater (p<0.01) in the boars with high (64%), compared to the boars with low protein content in seminal plasma (48%). The number of good diluted semen samples (>= 65% progressive motility), was also significantly (p<0.01) greater in the boars with high (41%), compared to the boars with low protein content in seminal plasma (12%). These results show that seminal plasma proteins play an important role in maintaining the sperm progressive motility of diluted semen in vitro stored for 3 days.",
publisher = "Slovak Univ Agriculture Nitra, Nitra",
journal = "Journal of Microbiology Biotechnology and Food Sciences",
title = "Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days",
volume = "4",
pages = "1-3",
doi = "10.15414/jmbfs.2015.4.special2.1-3"
}

Apić, J., Vakanjac, S., Radović, I., Jotanović, S., Živkov-Baloš, M., Milovanović, A., Barna, T., Stanković, B., Jajić, I.,& Maletić, M.. (2015). Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days. in Journal of Microbiology Biotechnology and Food Sciences
Slovak Univ Agriculture Nitra, Nitra., 4, 1-3.
https://doi.org/10.15414/jmbfs.2015.4.special2.1-3

Apić J, Vakanjac S, Radović I, Jotanović S, Živkov-Baloš M, Milovanović A, Barna T, Stanković B, Jajić I, Maletić M. Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days. in Journal of Microbiology Biotechnology and Food Sciences. 2015;4:1-3.
doi:10.15414/jmbfs.2015.4.special2.1-3 .

Apić, Jelena, Vakanjac, Slobodanka, Radović, Ivan, Jotanović, Stoja, Živkov-Baloš, Milica, Milovanović, Aleksandar, Barna, Tomislav, Stanković, Branislav, Jajić, Igor, Maletić, Milan, "Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days" in Journal of Microbiology Biotechnology and Food Sciences, 4 (2015):1-3,
https://doi.org/10.15414/jmbfs.2015.4.special2.1-3 . .

TY  - JOUR
AU  - Apić, Jelena
AU  - Vakanjac, Slobodanka
AU  - Radović, I.
AU  - Kucević, D.
AU  - Jotanović, Stoja
AU  - Kanacki, Z.
AU  - Stanković, B.
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1278
AB  - The aim of this study was to investigate: (a) seminal plasma protein content in the Land-race (n = 38), Large White (n = 44) and Duroc (n = 24) boars breed, as well as boars distribution according to protein content in seminal plasma, and (b) the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17°C for 3 days. Not significant (p > 0.05) differences was found in seminal plasma protein content between three boar breeds. Only 31% of the 106 investigated boars had > 3.6% protein in seminal plasma. Progressive motility was significantly higher (p  lt  0.01) in the ejaculates with high, compared to the ejaculates with low protein content (82% vs. 76%). After 3 days of storage, in thel:4 dilution proportion, the average progressive motility was significantly (p  lt  0.01) decreased in relation to this value in native semen from the boars with high (82% to 64%), as well from the boars with low protein content in seminal plasma (76% to 48%). However, the average diluted semen progressive motility was significantly greater (p  lt  0.01) in the boars with high (64%), compared to the boars with low protein content in seminal plasma (48%). The number of good diluted semen samples (>65% progressive motility), was also significantly (p  lt  0.01) greater in the boars with high (41%), compared to the boars with low protein content in seminal plasma (12%).
PB  - Eugen Ulmer Gmbh Co, Stuttgart
T2  - Zuchtungskunde
T1  - Proteine content in boar seminal plasma on the intensive pig production farms in Serbia
T1  - Proteingehalt im Samenplasma von Zuchtebern auf den Betrieben für intensive Schweineproduktion in Serbien
VL  - 87
IS  - 5
SP  - 361
EP  - 368
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3847
ER  - 

@article{
author = "Apić, Jelena and Vakanjac, Slobodanka and Radović, I. and Kucević, D. and Jotanović, Stoja and Kanacki, Z. and Stanković, B.",
year = "2015",
abstract = "The aim of this study was to investigate: (a) seminal plasma protein content in the Land-race (n = 38), Large White (n = 44) and Duroc (n = 24) boars breed, as well as boars distribution according to protein content in seminal plasma, and (b) the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17°C for 3 days. Not significant (p > 0.05) differences was found in seminal plasma protein content between three boar breeds. Only 31% of the 106 investigated boars had > 3.6% protein in seminal plasma. Progressive motility was significantly higher (p  lt  0.01) in the ejaculates with high, compared to the ejaculates with low protein content (82% vs. 76%). After 3 days of storage, in thel:4 dilution proportion, the average progressive motility was significantly (p  lt  0.01) decreased in relation to this value in native semen from the boars with high (82% to 64%), as well from the boars with low protein content in seminal plasma (76% to 48%). However, the average diluted semen progressive motility was significantly greater (p  lt  0.01) in the boars with high (64%), compared to the boars with low protein content in seminal plasma (48%). The number of good diluted semen samples (>65% progressive motility), was also significantly (p  lt  0.01) greater in the boars with high (41%), compared to the boars with low protein content in seminal plasma (12%).",
publisher = "Eugen Ulmer Gmbh Co, Stuttgart",
journal = "Zuchtungskunde",
title = "Proteine content in boar seminal plasma on the intensive pig production farms in Serbia, Proteingehalt im Samenplasma von Zuchtebern auf den Betrieben für intensive Schweineproduktion in Serbien",
volume = "87",
number = "5",
pages = "361-368",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3847"
}

Apić, J., Vakanjac, S., Radović, I., Kucević, D., Jotanović, S., Kanacki, Z.,& Stanković, B.. (2015). Proteine content in boar seminal plasma on the intensive pig production farms in Serbia. in Zuchtungskunde
Eugen Ulmer Gmbh Co, Stuttgart., 87(5), 361-368.
https://hdl.handle.net/21.15107/rcub_agrospace_3847

Apić J, Vakanjac S, Radović I, Kucević D, Jotanović S, Kanacki Z, Stanković B. Proteine content in boar seminal plasma on the intensive pig production farms in Serbia. in Zuchtungskunde. 2015;87(5):361-368.
https://hdl.handle.net/21.15107/rcub_agrospace_3847 .

Apić, Jelena, Vakanjac, Slobodanka, Radović, I., Kucević, D., Jotanović, Stoja, Kanacki, Z., Stanković, B., "Proteine content in boar seminal plasma on the intensive pig production farms in Serbia" in Zuchtungskunde, 87, no. 5 (2015):361-368,
https://hdl.handle.net/21.15107/rcub_agrospace_3847 .

TY  - JOUR
AU  - Apić, Jelena
AU  - Vakanjac, Slobodanka
AU  - Radović, Ivan
AU  - Jotanović, Stoja
AU  - Stanković, Branislav
AU  - Kanački, Zdenko
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1300
AB  - It was demonstrated that boar sperm production and characteristics significantly decrease in the wormer period of the year. As a result, the boars reproductive exploitation efficiency decrease in the wormer season. Therefore, the aim of the present paper was to investigate the influence of cold and warm season on the boars semen quality, in the our intensive pig production conditions. The obtained results clearly show that the main semen quality parameters were significantly (p lt 0.05 or p lt 0.01) higher in the cold, compared with the warm season (ejaculate volume=274ml, sperm concentration=229'106/ml, total sperm number=60'109, progressive motility=79%, and good ejaculates=96%, vs. 218ml, 208'106/ml, 45'109, 69% and 78%, resp.). In conclusion, keeping boars cool during summer and frequent observation of boars to determine if they are being heat- stressed, can significant reduce the negative influence of elevated ambient temperature on sperm production and, consequently, improve boars reproductive exploitation efficiency.
AB  - Ustanovljeno je da su produkcija i osobine sperme nerasta značajno niže tokom toplijeg perioda godine. Posledično, u ovom periodu godine značajno se smanjuje efikasnost reproduktivnog iskorištavanja nerastova. Zbog toga je cilj ovog rada bio da se ustanovi uticaj hladne i tople godišnje sezone na parametre kvaliteta sperme u našim uslovima intenzivne proizvodnje svinja. Dobijeni rezultati jasno pokazuju da su osnovni parametri kvaliteta ejakulata značajno veći (p lt 0.05 or p lt 0.01) u hladnoj, nego u toploj godišnjoj sezoni (volumen ejakulata=274ml, koncentracija spermatozoida=229'106/ml, ukupan broj spermatozoida=60'109, progresivna pokretljivost=79% i dobrih ejakulata=96%, prema 218ml, 208'106/ml, 45'109, 69% i 78%). Zaključak je da rashlađivanje nerastova tokom leta i češća observacija prisustva toplotnog stresa, može značajno smanjiti negativan uticaj povišene ambijentalne temperature na produkciju sperme i, posledično, povećati efikasnost reproduktivnog iskorištavanja nerastova.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Savremena poljoprivreda
T1  - Effect of season on boast semen quality
T1  - Uticaj sezone na kvalitet sperme nerasta
VL  - 64
IS  - 1-2
SP  - 9
EP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3935
ER  - 

@article{
author = "Apić, Jelena and Vakanjac, Slobodanka and Radović, Ivan and Jotanović, Stoja and Stanković, Branislav and Kanački, Zdenko",
year = "2015",
abstract = "It was demonstrated that boar sperm production and characteristics significantly decrease in the wormer period of the year. As a result, the boars reproductive exploitation efficiency decrease in the wormer season. Therefore, the aim of the present paper was to investigate the influence of cold and warm season on the boars semen quality, in the our intensive pig production conditions. The obtained results clearly show that the main semen quality parameters were significantly (p lt 0.05 or p lt 0.01) higher in the cold, compared with the warm season (ejaculate volume=274ml, sperm concentration=229'106/ml, total sperm number=60'109, progressive motility=79%, and good ejaculates=96%, vs. 218ml, 208'106/ml, 45'109, 69% and 78%, resp.). In conclusion, keeping boars cool during summer and frequent observation of boars to determine if they are being heat- stressed, can significant reduce the negative influence of elevated ambient temperature on sperm production and, consequently, improve boars reproductive exploitation efficiency., Ustanovljeno je da su produkcija i osobine sperme nerasta značajno niže tokom toplijeg perioda godine. Posledično, u ovom periodu godine značajno se smanjuje efikasnost reproduktivnog iskorištavanja nerastova. Zbog toga je cilj ovog rada bio da se ustanovi uticaj hladne i tople godišnje sezone na parametre kvaliteta sperme u našim uslovima intenzivne proizvodnje svinja. Dobijeni rezultati jasno pokazuju da su osnovni parametri kvaliteta ejakulata značajno veći (p lt 0.05 or p lt 0.01) u hladnoj, nego u toploj godišnjoj sezoni (volumen ejakulata=274ml, koncentracija spermatozoida=229'106/ml, ukupan broj spermatozoida=60'109, progresivna pokretljivost=79% i dobrih ejakulata=96%, prema 218ml, 208'106/ml, 45'109, 69% i 78%). Zaključak je da rashlađivanje nerastova tokom leta i češća observacija prisustva toplotnog stresa, može značajno smanjiti negativan uticaj povišene ambijentalne temperature na produkciju sperme i, posledično, povećati efikasnost reproduktivnog iskorištavanja nerastova.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Savremena poljoprivreda",
title = "Effect of season on boast semen quality, Uticaj sezone na kvalitet sperme nerasta",
volume = "64",
number = "1-2",
pages = "9-13",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3935"
}

Apić, J., Vakanjac, S., Radović, I., Jotanović, S., Stanković, B.,& Kanački, Z.. (2015). Effect of season on boast semen quality. in Savremena poljoprivreda
Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 64(1-2), 9-13.
https://hdl.handle.net/21.15107/rcub_agrospace_3935

Apić J, Vakanjac S, Radović I, Jotanović S, Stanković B, Kanački Z. Effect of season on boast semen quality. in Savremena poljoprivreda. 2015;64(1-2):9-13.
https://hdl.handle.net/21.15107/rcub_agrospace_3935 .

Apić, Jelena, Vakanjac, Slobodanka, Radović, Ivan, Jotanović, Stoja, Stanković, Branislav, Kanački, Zdenko, "Effect of season on boast semen quality" in Savremena poljoprivreda, 64, no. 1-2 (2015):9-13,
https://hdl.handle.net/21.15107/rcub_agrospace_3935 .

TY  - JOUR
AU  - Apić, Jelena
AU  - Vakanjac, Slobodanka
AU  - Stančić, Ivan
AU  - Radović, Ivan
AU  - Jotanović, Stoja
AU  - Kanacki, Zdenko
AU  - Stanković, Branislav
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1275
AB  - The aim of the present study was to investigate the possibility of increasing boars reproductive exploitation by using AI doses of doubly reduced volume and sperm count in the intrauterine AI procedure. The experiment was conducted at a commercial pig farm in Serbia in 2014. Classic intracervical insemination (ICI) was performed by using 50 mL or 100 mL volume doses containing 4 x 10(9) or 2 x 10(9) progressively motile spermatozoa. The same volumes and sperm numbers per dose were used with intrauterine insemination (IUI). Each dose combination was used to inseminate 30 sows. Intrauterine insemination with AI doses of reduced volume (50 mL) and sperm count (2 x 10(9)) did not produce a statistically significant difference (P < 0.05) in the farrowing rate (76.7%) as compared with 4 x 10(9) spermatozoa in the same volume (83.3%) or with insemination by doses of 100 mL with a 2 x 10(9) (83.3%) or a 4 x 10(9) sperm count (86.7%). The number of live-born piglets (10.82) was larger following IUI using a 50 mL volume dose with a 2 x 10(9) sperm count as compared with ICI with the same AI dose volume and sperm count (9.85). The results show that the use of reduced AI dosages provides an opportunity for the swine industry to considerably exploit the reproductive potential of genetically superior boars.
PB  - Scientific Technical Research Council Turkey-Tubitak, Ankara
T2  - Turkish Journal of Veterinary & Animal Sciences
T1  - Sow fertility after insemination with varying doses of volume and spermatozoa count
VL  - 39
IS  - 6
SP  - 709
EP  - 713
DO  - 10.3906/vet-1503-50
ER  - 

@article{
author = "Apić, Jelena and Vakanjac, Slobodanka and Stančić, Ivan and Radović, Ivan and Jotanović, Stoja and Kanacki, Zdenko and Stanković, Branislav",
year = "2015",
abstract = "The aim of the present study was to investigate the possibility of increasing boars reproductive exploitation by using AI doses of doubly reduced volume and sperm count in the intrauterine AI procedure. The experiment was conducted at a commercial pig farm in Serbia in 2014. Classic intracervical insemination (ICI) was performed by using 50 mL or 100 mL volume doses containing 4 x 10(9) or 2 x 10(9) progressively motile spermatozoa. The same volumes and sperm numbers per dose were used with intrauterine insemination (IUI). Each dose combination was used to inseminate 30 sows. Intrauterine insemination with AI doses of reduced volume (50 mL) and sperm count (2 x 10(9)) did not produce a statistically significant difference (P < 0.05) in the farrowing rate (76.7%) as compared with 4 x 10(9) spermatozoa in the same volume (83.3%) or with insemination by doses of 100 mL with a 2 x 10(9) (83.3%) or a 4 x 10(9) sperm count (86.7%). The number of live-born piglets (10.82) was larger following IUI using a 50 mL volume dose with a 2 x 10(9) sperm count as compared with ICI with the same AI dose volume and sperm count (9.85). The results show that the use of reduced AI dosages provides an opportunity for the swine industry to considerably exploit the reproductive potential of genetically superior boars.",
publisher = "Scientific Technical Research Council Turkey-Tubitak, Ankara",
journal = "Turkish Journal of Veterinary & Animal Sciences",
title = "Sow fertility after insemination with varying doses of volume and spermatozoa count",
volume = "39",
number = "6",
pages = "709-713",
doi = "10.3906/vet-1503-50"
}

Apić, J., Vakanjac, S., Stančić, I., Radović, I., Jotanović, S., Kanacki, Z.,& Stanković, B.. (2015). Sow fertility after insemination with varying doses of volume and spermatozoa count. in Turkish Journal of Veterinary & Animal Sciences
Scientific Technical Research Council Turkey-Tubitak, Ankara., 39(6), 709-713.
https://doi.org/10.3906/vet-1503-50

Apić J, Vakanjac S, Stančić I, Radović I, Jotanović S, Kanacki Z, Stanković B. Sow fertility after insemination with varying doses of volume and spermatozoa count. in Turkish Journal of Veterinary & Animal Sciences. 2015;39(6):709-713.
doi:10.3906/vet-1503-50 .

Apić, Jelena, Vakanjac, Slobodanka, Stančić, Ivan, Radović, Ivan, Jotanović, Stoja, Kanacki, Zdenko, Stanković, Branislav, "Sow fertility after insemination with varying doses of volume and spermatozoa count" in Turkish Journal of Veterinary & Animal Sciences, 39, no. 6 (2015):709-713,
https://doi.org/10.3906/vet-1503-50 . .

TY  - CONF
AU  - Milovanović, A.
AU  - Bana, T.
AU  - Lazarević, Miodrag
AU  - Apić, Jelena
AU  - Milanov, Dubravka
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1303
AB  - Importing boars of high genetic value is an important issue in Serbia.
Therefore, frequent replacement of boars is not a primary approach
and there is a need for their prolonged exploitation. Ejaculates from
6 boars were selected for bacterial control and for treatment based
on lower CASA (Computer Assisted Semen Analyses) results, flow
cytometry and cytological sperm quality parameters in extended
semen. According to the sensitivity of bacterial isolates from fresh
semen, boars were treated with Enroxil  10% (1 ml/40 kg BW;
Krka, Slovenia), during 8 consecutive days. An antibiotic was
injected between the tail and rectum (pararectal therapy). On the 8th
day, boars received 8 ml of Baytril  10% (Bayer, Germany). On the
first and last day of treatment boars received 20 ml of AD3E
vitamins with selenium i/m and C vitamin (20 ml, 100 mg/ml).
Semen quality was controlled 45 days following treatment. Total and
progressive sperm motility increased from 39.66% and 12.87% to
83.98% and 61.98%, respectively (p < 0.001). The percentage of live
spermatozoa with intact acrosomes also improved (48.3–63.4%,
PNA-FITC, n.s.). The percentage of agglutinated cells decreased
from 66.00 to 6.67% and the average bacterial contamination in
fresh semen decreased from 18.450 to 1.233 CFU/ml. It is evident
that boars with primary semen contamination are able to renew
sperm production after prolonged antibiotic and supportive treatment. This could be a strategy to decrease the culling rate in elite
stud boars.
PB  - Wiley-Blackwell, Hoboken
C3  - Reproduction in Domestic Animals
T1  - Pararectal treatment of boars with primary semen contamination and astenospermia
VL  - 50
SP  - 67
EP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1303
ER  - 

@conference{
author = "Milovanović, A. and Bana, T. and Lazarević, Miodrag and Apić, Jelena and Milanov, Dubravka",
year = "2015",
abstract = "Importing boars of high genetic value is an important issue in Serbia.
Therefore, frequent replacement of boars is not a primary approach
and there is a need for their prolonged exploitation. Ejaculates from
6 boars were selected for bacterial control and for treatment based
on lower CASA (Computer Assisted Semen Analyses) results, flow
cytometry and cytological sperm quality parameters in extended
semen. According to the sensitivity of bacterial isolates from fresh
semen, boars were treated with Enroxil  10% (1 ml/40 kg BW;
Krka, Slovenia), during 8 consecutive days. An antibiotic was
injected between the tail and rectum (pararectal therapy). On the 8th
day, boars received 8 ml of Baytril  10% (Bayer, Germany). On the
first and last day of treatment boars received 20 ml of AD3E
vitamins with selenium i/m and C vitamin (20 ml, 100 mg/ml).
Semen quality was controlled 45 days following treatment. Total and
progressive sperm motility increased from 39.66% and 12.87% to
83.98% and 61.98%, respectively (p < 0.001). The percentage of live
spermatozoa with intact acrosomes also improved (48.3–63.4%,
PNA-FITC, n.s.). The percentage of agglutinated cells decreased
from 66.00 to 6.67% and the average bacterial contamination in
fresh semen decreased from 18.450 to 1.233 CFU/ml. It is evident
that boars with primary semen contamination are able to renew
sperm production after prolonged antibiotic and supportive treatment. This could be a strategy to decrease the culling rate in elite
stud boars.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Reproduction in Domestic Animals",
title = "Pararectal treatment of boars with primary semen contamination and astenospermia",
volume = "50",
pages = "67-67",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1303"
}

Milovanović, A., Bana, T., Lazarević, M., Apić, J.,& Milanov, D.. (2015). Pararectal treatment of boars with primary semen contamination and astenospermia. in Reproduction in Domestic Animals
Wiley-Blackwell, Hoboken., 50, 67-67.
https://hdl.handle.net/21.15107/rcub_veterinar_1303

Milovanović A, Bana T, Lazarević M, Apić J, Milanov D. Pararectal treatment of boars with primary semen contamination and astenospermia. in Reproduction in Domestic Animals. 2015;50:67-67.
https://hdl.handle.net/21.15107/rcub_veterinar_1303 .

Milovanović, A., Bana, T., Lazarević, Miodrag, Apić, Jelena, Milanov, Dubravka, "Pararectal treatment of boars with primary semen contamination and astenospermia" in Reproduction in Domestic Animals, 50 (2015):67-67,
https://hdl.handle.net/21.15107/rcub_veterinar_1303 .

TY  - JOUR
AU  - Ćirković, Miroslav
AU  - Novakov, Nikolina
AU  - Petrović, Jelena
AU  - Ljubojević, Dragana
AU  - Apić, Jelena
AU  - Babić, Jelena
AU  - Teodorović, Vlado
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1067
AB  - When placing the fish and fish products on the trade it is necessary to pay attention to the presence of zoonotic parasites that can lead to infection of people especially if the fish is consumed raw or undercooked. Epidemiologically the most important are helminths from the group of Nematoda. The most important are Anisaxis spp. and Eustrongylides excisus. Anisakiasis is a serious zoonotic disease with a dramatic increase in prevalence throughout the world in the last two decades. The larvae are found in marine fish species most frequently in sardines, herring and mackerel. Eustrongylidosis is a disease that occurs primarily in freshwater fish species: catfish, zander and pike, a cause is a parasite of the genus Eustrongylides of which the most important species is Eustrongylides excisus. In this paper, the nematodes that occur in fish that are present in the market in the Republic of Serbia are present. Investigations of Anisaxis spp. were carried out at the Institute of Veterinary Medicine Novi Sad in the period of 2000-2013. Total of 2414 samples of imported marine fish was inspected. In 25 (1.29 % ) samples of herrings (Clupea harengus ) and mackerels ( Scomber scombrus ) was identified Anisakis spp. Investigations of Eustrongylides spp . were carried out in the period 2010-2013 at the Danube-Tisa-Danube Canal. Samples were collected from zander (Sander lucioperca) and European catfish (Silurisglanis) in which the presence of nematodes was found in the abdominal cavity, muscles, the lumen of the stomach and gastric wall where the parasites were encapsulated. Individuals of zander were examined during the 2013 at the Veterinary Institute Novi Sad, where the presence of larvae Eustrongylides spp. u muscle was detected. In order to avoid infecting people with parasites of fish it is necessary to carry out continuous control and monitoring. Fresh fishes and traditional fish products must be inspected for the presence of parasites before they find on the trade. Continuous education is a key factor in combating zoonotic diseases. Avoid consumption of raw or poorly cooked fish is still the best preventive procedure.
AB  - Prilikom stavljanja u promet i pregleda ribljeg mesa i proizvoda od ribe treba obratiti pažnju na prisustvo zoonotskih parazita koji mogu dovesti do inficiranja ljudi naročito ako se riba konzumira sirova ili termički nedovoljno obrađena. Sa epidemiološkog aspekta najveći značaj imaju helminti iz grupe Nematoda. Najznačajnije su Anisaxis spp. i Eustrongylides excisus. Anisakijaza je ozbiljno zoonotsko oboljenje sa dramatičnim porastom prevalence širom sveta u poslednje dve decenije. Larve se mogu naći kod morskih vrsta riba najčešće kod sardine, haringe i skuše. Eustrongilidoza je oboljenje koje se javlja kod slatkovodnih riba prvenstveno grabljivica: soma, smuđa i štuke, a izazivaju je paraziti iz roda Eustrongylides od kojih je najznačajnija vrsta Eustrongylides excisus. U ovom radu prikazane su nematode koje se javljaju kod riba koje su prisutne na tržištu u Republici Srbiji. Istraživanja prisustva Anisaxis spp. su sprovedena na Naučnom institutu za veterinarstvo Novi Sad u periodu 2000-2013. Ukupno je pregledano 2414 uzoraka riba iz uvoza od čega je u 25 (1.29%) utvrđen Anisakis spp., kod haringe (Clupea harengus) i skuše (Scomber scombrus). Istraživanja prisustva Eustrongylides spp. su sprovedena u periodu od 2010-2013 godine na kanalu Dunav-Tisa-Dunav. Prikupljeni su uzorci konzumnog smuđa (Sander lucioperca) i soma (Siluris glanis) kod kojih je utvrđeno prisustvo nematoda u abdomenu, muskulaturi, lumenu želuca i želudačnom zidu gde su paraziti bili inkapsulirani. Jedinke smuda pregledane se tokom 2013. godine i na Naučnom Institutu za veterinarstvo Novi Sad gde je utvrđeno prisustvo larvi Eustrongylides spp. u muskulaturi. Kako ne bi došlo do zaražavanja ljudi zoonotskim parazitima riba neophodno je sprovoditi stalnu kontrolu i monitoring. Sveže meso ribe i tradicionalni riblji proizvodi pre nego što se nadu u prometu moraju biti pregledani na prisustvo parazita. Kontinuirana edukacija je ključni faktor u borbi sa zoonozama a izbegavanje konzumiranja sirovog ili termički slabo obrađenog ribljeg mesa i dalje najbolja preventivna procedura.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Finding of parasitic nematodes of fishes present in the market
T1  - Nalaženje parazitskih nematoda kod riba na tržištu
VL  - 6
IS  - 2
SP  - 3
EP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1067
ER  - 

@article{
author = "Ćirković, Miroslav and Novakov, Nikolina and Petrović, Jelena and Ljubojević, Dragana and Apić, Jelena and Babić, Jelena and Teodorović, Vlado",
year = "2013",
abstract = "When placing the fish and fish products on the trade it is necessary to pay attention to the presence of zoonotic parasites that can lead to infection of people especially if the fish is consumed raw or undercooked. Epidemiologically the most important are helminths from the group of Nematoda. The most important are Anisaxis spp. and Eustrongylides excisus. Anisakiasis is a serious zoonotic disease with a dramatic increase in prevalence throughout the world in the last two decades. The larvae are found in marine fish species most frequently in sardines, herring and mackerel. Eustrongylidosis is a disease that occurs primarily in freshwater fish species: catfish, zander and pike, a cause is a parasite of the genus Eustrongylides of which the most important species is Eustrongylides excisus. In this paper, the nematodes that occur in fish that are present in the market in the Republic of Serbia are present. Investigations of Anisaxis spp. were carried out at the Institute of Veterinary Medicine Novi Sad in the period of 2000-2013. Total of 2414 samples of imported marine fish was inspected. In 25 (1.29 % ) samples of herrings (Clupea harengus ) and mackerels ( Scomber scombrus ) was identified Anisakis spp. Investigations of Eustrongylides spp . were carried out in the period 2010-2013 at the Danube-Tisa-Danube Canal. Samples were collected from zander (Sander lucioperca) and European catfish (Silurisglanis) in which the presence of nematodes was found in the abdominal cavity, muscles, the lumen of the stomach and gastric wall where the parasites were encapsulated. Individuals of zander were examined during the 2013 at the Veterinary Institute Novi Sad, where the presence of larvae Eustrongylides spp. u muscle was detected. In order to avoid infecting people with parasites of fish it is necessary to carry out continuous control and monitoring. Fresh fishes and traditional fish products must be inspected for the presence of parasites before they find on the trade. Continuous education is a key factor in combating zoonotic diseases. Avoid consumption of raw or poorly cooked fish is still the best preventive procedure., Prilikom stavljanja u promet i pregleda ribljeg mesa i proizvoda od ribe treba obratiti pažnju na prisustvo zoonotskih parazita koji mogu dovesti do inficiranja ljudi naročito ako se riba konzumira sirova ili termički nedovoljno obrađena. Sa epidemiološkog aspekta najveći značaj imaju helminti iz grupe Nematoda. Najznačajnije su Anisaxis spp. i Eustrongylides excisus. Anisakijaza je ozbiljno zoonotsko oboljenje sa dramatičnim porastom prevalence širom sveta u poslednje dve decenije. Larve se mogu naći kod morskih vrsta riba najčešće kod sardine, haringe i skuše. Eustrongilidoza je oboljenje koje se javlja kod slatkovodnih riba prvenstveno grabljivica: soma, smuđa i štuke, a izazivaju je paraziti iz roda Eustrongylides od kojih je najznačajnija vrsta Eustrongylides excisus. U ovom radu prikazane su nematode koje se javljaju kod riba koje su prisutne na tržištu u Republici Srbiji. Istraživanja prisustva Anisaxis spp. su sprovedena na Naučnom institutu za veterinarstvo Novi Sad u periodu 2000-2013. Ukupno je pregledano 2414 uzoraka riba iz uvoza od čega je u 25 (1.29%) utvrđen Anisakis spp., kod haringe (Clupea harengus) i skuše (Scomber scombrus). Istraživanja prisustva Eustrongylides spp. su sprovedena u periodu od 2010-2013 godine na kanalu Dunav-Tisa-Dunav. Prikupljeni su uzorci konzumnog smuđa (Sander lucioperca) i soma (Siluris glanis) kod kojih je utvrđeno prisustvo nematoda u abdomenu, muskulaturi, lumenu želuca i želudačnom zidu gde su paraziti bili inkapsulirani. Jedinke smuda pregledane se tokom 2013. godine i na Naučnom Institutu za veterinarstvo Novi Sad gde je utvrđeno prisustvo larvi Eustrongylides spp. u muskulaturi. Kako ne bi došlo do zaražavanja ljudi zoonotskim parazitima riba neophodno je sprovoditi stalnu kontrolu i monitoring. Sveže meso ribe i tradicionalni riblji proizvodi pre nego što se nadu u prometu moraju biti pregledani na prisustvo parazita. Kontinuirana edukacija je ključni faktor u borbi sa zoonozama a izbegavanje konzumiranja sirovog ili termički slabo obrađenog ribljeg mesa i dalje najbolja preventivna procedura.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Finding of parasitic nematodes of fishes present in the market, Nalaženje parazitskih nematoda kod riba na tržištu",
volume = "6",
number = "2",
pages = "3-13",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1067"
}

Ćirković, M., Novakov, N., Petrović, J., Ljubojević, D., Apić, J., Babić, J.,& Teodorović, V.. (2013). Finding of parasitic nematodes of fishes present in the market. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 6(2), 3-13.
https://hdl.handle.net/21.15107/rcub_veterinar_1067

Ćirković M, Novakov N, Petrović J, Ljubojević D, Apić J, Babić J, Teodorović V. Finding of parasitic nematodes of fishes present in the market. in Arhiv veterinarske medicine. 2013;6(2):3-13.
https://hdl.handle.net/21.15107/rcub_veterinar_1067 .

Ćirković, Miroslav, Novakov, Nikolina, Petrović, Jelena, Ljubojević, Dragana, Apić, Jelena, Babić, Jelena, Teodorović, Vlado, "Finding of parasitic nematodes of fishes present in the market" in Arhiv veterinarske medicine, 6, no. 2 (2013):3-13,
https://hdl.handle.net/21.15107/rcub_veterinar_1067 .