TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Aleksić-Kovačević, Sanja
AU  - Savić, Božidar
AU  - Prodanović, Radiša
AU  - Jezdimirović, Nemanja
AU  - Milićević, Vesna
AU  - Maksimović Zorić, Jelena
AU  - Kureljušić, Jasna
AU  - Žutić, Jadranka
AU  - Knežević, Đorđe
AU  - Spalević, Ljiljana
AU  - Kukolj, Vladimir
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2784
AB  - Hepatitis E virus (HEV), the zoonotic agent of infectious hepatitis, is present in swine farms
in different geographical areas. Little is known about the mechanism of liver damage and
type of local immune response by HEV in swine. Therefore, the aim of this study was to
determine the morphological and immunophenotypic characteristics of hepatic lesions
caused by hepatitis E virus in naturally infected swine. In this study, liver samples of 12
slaughtered 10 weeks old pigs which were RT‑PCR positive for HEV RNA in rectal swab
samples have been used. Livers were macroscopically examined and samples were taken
for histopathological, immunohistochemical (CD3, CD79α and TGF‑β1), semiquantitative,
morphometric analysis, RT‑nested‑PCR, PCR and bacteriological analysis. Microscopically,
mild and moderate multifocal lymphoplasmacytic hepatitis was observed. Apoptotic bodies
were observed as areas of focal eosinophilic condensation in the cytoplasm of 33.33% liver
samples, while in 16.67% liver samples portal fibrosis was detected. Immunohistochemically,
portal and lobular lymphocytes in the mononuclear liver infiltrate were predominantly
CD3+ T cells (234.80 ± 79.98). An intense TGF‑β1 positive reaction was observed within the
mononuclear cell infiltrate as well as polymorphonuclear cells in liver samples with apoptosis
of hepatocytes. In all 12 tested liver samples HEV RNA was detected by RT‑nested‑PCR.
HEV is noncytopathic, and this finding provides further evidence for an immune mediated
pathogenesis in hepatitis E virus infection in swine. Also, the role of CD3+ cells in hepatocyte
damage is clearly demonstrated.
PB  - Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise)
T2  - Veterinaria Italiana
T1  - Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus
VL  - 54
IS  - 7
SP  - 287
EP  - 295
DO  - 10.12834/VetIt.1813.9553.3
ER  - 

@article{
author = "Kureljušić, Branislav and Aleksić-Kovačević, Sanja and Savić, Božidar and Prodanović, Radiša and Jezdimirović, Nemanja and Milićević, Vesna and Maksimović Zorić, Jelena and Kureljušić, Jasna and Žutić, Jadranka and Knežević, Đorđe and Spalević, Ljiljana and Kukolj, Vladimir",
year = "2021",
abstract = "Hepatitis E virus (HEV), the zoonotic agent of infectious hepatitis, is present in swine farms
in different geographical areas. Little is known about the mechanism of liver damage and
type of local immune response by HEV in swine. Therefore, the aim of this study was to
determine the morphological and immunophenotypic characteristics of hepatic lesions
caused by hepatitis E virus in naturally infected swine. In this study, liver samples of 12
slaughtered 10 weeks old pigs which were RT‑PCR positive for HEV RNA in rectal swab
samples have been used. Livers were macroscopically examined and samples were taken
for histopathological, immunohistochemical (CD3, CD79α and TGF‑β1), semiquantitative,
morphometric analysis, RT‑nested‑PCR, PCR and bacteriological analysis. Microscopically,
mild and moderate multifocal lymphoplasmacytic hepatitis was observed. Apoptotic bodies
were observed as areas of focal eosinophilic condensation in the cytoplasm of 33.33% liver
samples, while in 16.67% liver samples portal fibrosis was detected. Immunohistochemically,
portal and lobular lymphocytes in the mononuclear liver infiltrate were predominantly
CD3+ T cells (234.80 ± 79.98). An intense TGF‑β1 positive reaction was observed within the
mononuclear cell infiltrate as well as polymorphonuclear cells in liver samples with apoptosis
of hepatocytes. In all 12 tested liver samples HEV RNA was detected by RT‑nested‑PCR.
HEV is noncytopathic, and this finding provides further evidence for an immune mediated
pathogenesis in hepatitis E virus infection in swine. Also, the role of CD3+ cells in hepatocyte
damage is clearly demonstrated.",
publisher = "Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise)",
journal = "Veterinaria Italiana",
title = "Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus",
volume = "54",
number = "7",
pages = "287-295",
doi = "10.12834/VetIt.1813.9553.3"
}

Kureljušić, B., Aleksić-Kovačević, S., Savić, B., Prodanović, R., Jezdimirović, N., Milićević, V., Maksimović Zorić, J., Kureljušić, J., Žutić, J., Knežević, Đ., Spalević, L.,& Kukolj, V.. (2021). Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus. in Veterinaria Italiana
Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise)., 54(7), 287-295.
https://doi.org/10.12834/VetIt.1813.9553.3

Kureljušić B, Aleksić-Kovačević S, Savić B, Prodanović R, Jezdimirović N, Milićević V, Maksimović Zorić J, Kureljušić J, Žutić J, Knežević Đ, Spalević L, Kukolj V. Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus. in Veterinaria Italiana. 2021;54(7):287-295.
doi:10.12834/VetIt.1813.9553.3 .

Kureljušić, Branislav, Aleksić-Kovačević, Sanja, Savić, Božidar, Prodanović, Radiša, Jezdimirović, Nemanja, Milićević, Vesna, Maksimović Zorić, Jelena, Kureljušić, Jasna, Žutić, Jadranka, Knežević, Đorđe, Spalević, Ljiljana, Kukolj, Vladimir, "Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus" in Veterinaria Italiana, 54, no. 7 (2021):287-295,
https://doi.org/10.12834/VetIt.1813.9553.3 . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Kureljušić, Branislav
AU  - Maksimović-Zorić, Jelena
AU  - Savić, Božidar
AU  - Spalević, Ljiljana
AU  - Žutić, Jadranka
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1985
AB  - Porcine Kobuvirus (PKV) infection is very common in pigs throughout the world. Since it has never been investigated in Serbia, to contribute to the knowledge of Porcine Kobuvirus, its role, and distribution, we tested 200 samples from domestic pigs and wild boars. From domestic pigs, 10 fecal, 22 spleen and 68 serum samples, and 100 spleen samples from wild boars were tested. The virus prevalence determined by real-time RT-PCR in domestic pigs was 22% and in wild boars 6%. The phylogenetic analysis of 3D region revealed that Serbian strains are closest related to the Hungarian strain from wild boar from 2011. This is the first report on PKV in Serbia in domestic pigs and wild boars, implying its wide circulation. Although the infection could not be directly related to any clinical manifestation, the frequency of virus found in feces suggests viral affinity to the gastrointestinal tract. However, due to the rather ubiquitous presence of PKV, the clinical and pathological assessment have to be considered when PKV infection is diagnosed.
PB  - Elsevier
T2  - Research in Veterinary Science
T1  - Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia
VL  - 132
SP  - 404
EP  - 406
DO  - 10.1016/j.rvsc.2020.07.028
ER  - 

@article{
author = "Milićević, Vesna and Kureljušić, Branislav and Maksimović-Zorić, Jelena and Savić, Božidar and Spalević, Ljiljana and Žutić, Jadranka",
year = "2020",
abstract = "Porcine Kobuvirus (PKV) infection is very common in pigs throughout the world. Since it has never been investigated in Serbia, to contribute to the knowledge of Porcine Kobuvirus, its role, and distribution, we tested 200 samples from domestic pigs and wild boars. From domestic pigs, 10 fecal, 22 spleen and 68 serum samples, and 100 spleen samples from wild boars were tested. The virus prevalence determined by real-time RT-PCR in domestic pigs was 22% and in wild boars 6%. The phylogenetic analysis of 3D region revealed that Serbian strains are closest related to the Hungarian strain from wild boar from 2011. This is the first report on PKV in Serbia in domestic pigs and wild boars, implying its wide circulation. Although the infection could not be directly related to any clinical manifestation, the frequency of virus found in feces suggests viral affinity to the gastrointestinal tract. However, due to the rather ubiquitous presence of PKV, the clinical and pathological assessment have to be considered when PKV infection is diagnosed.",
publisher = "Elsevier",
journal = "Research in Veterinary Science",
title = "Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia",
volume = "132",
pages = "404-406",
doi = "10.1016/j.rvsc.2020.07.028"
}

Milićević, V., Kureljušić, B., Maksimović-Zorić, J., Savić, B., Spalević, L.,& Žutić, J.. (2020). Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia. in Research in Veterinary Science
Elsevier., 132, 404-406.
https://doi.org/10.1016/j.rvsc.2020.07.028

Milićević V, Kureljušić B, Maksimović-Zorić J, Savić B, Spalević L, Žutić J. Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia. in Research in Veterinary Science. 2020;132:404-406.
doi:10.1016/j.rvsc.2020.07.028 .

Milićević, Vesna, Kureljušić, Branislav, Maksimović-Zorić, Jelena, Savić, Božidar, Spalević, Ljiljana, Žutić, Jadranka, "Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia" in Research in Veterinary Science, 132 (2020):404-406,
https://doi.org/10.1016/j.rvsc.2020.07.028 . .

TY  - JOUR
AU  - Maksimović Zorić, Jelena
AU  - Milićević, Vesna
AU  - Stevančević, Ognjen
AU  - Chiapponi, Chiara
AU  - Potkonjak, Aleksandar
AU  - Stojanac, Nenad
AU  - Kureljušić, Branislav
AU  - Veljović, Lubiša
AU  - Radosavljević, Vladimir
AU  - Savić, Božidar
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1987
AB  - Pigs are very important for the epidemiology of influenza A viruses, being commonly infected with the lineages of most adapted H1N1, H3N2, H1N2 swine subtypes. Epidemiological complexity of swine influenza is increasing by a periodic spillover of human or avian viruses in the pig population when genetic shifts can occur. The objectives of this research were to determine the presence of the influenza A virus in nasal and tracheobronchial swabs and lung tissue samples of ill and dead pigs on commercial farms, to determine circulating subtypes and characterize them through the phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes. A total of 255 samples collected from 13 farms were analyzed by means of real-time RT-PCR. The genome of influenza A virus was detected in 24 samples, which represented a 61.5% prevalence at the farms level (influenza A virus was confirmed in 8 out of 13 farms included in this study). Based on HA and NA gene sequences of 8 viruses, the circulation of H1N1 and H3N2 subtypes of influenza A viruses were determined. In addition, one farm exhibited a time separated circulation of H1N1 and H3N2 virus subtypes. Using Influenza Research Database, our viruses of the H1 subtype were classified into 1C.2.1 and 1A.3.3.2. clade. Based on the nucleotide sequences of HA genes, three viruses of the H1N1 subtype belong to the H1N1pdm09 lineage, and the other four to Eurasian “avian-like” H1avN1 lineage; while based on NA genes sequences, these seven viruses belong to Eurasian “avian-like” H1avN1 lineage. Both HA and NA genes of the virus of the H3N2 subtype belonged to the A/swine/ Gent/1/1984-like H3N2 lineage.
AB  - Svinje su veoma važne u epidemiologiji infl uenca A virusa, jer je većina zapata širom sveta zaražena nekim od tri podtipa (H1N1, H1N2 ili H3N2). Pored toga, one su prijemčive i za ljudske i ptičije infl eunca A viruse, usled čega u njihovom organizmu može doći do genetskog reasortiranja i stvaranja genotipski i fenotipski novih virusa. Cilj ovog istraživanja je bio da se analizom nosnih i traeo-bronhijalnih briseva i pluća poreklom od bolesnih i ugilnulih svinja ispita prisustvo infl uenca A virusa, da se odrede cirkulišući podtipovi i fi logenetski okarakterišu kroz analizu sekvenci HA i NA gena. Tokom ovog istraživanja sa 13 farmi je sakupljeno i metodom realtime RT-PCR pregledano je 255 uzoraka. Genom infl uenca A virusa je utvrđen u 24 uzorka. Prevalencija virusa na nivou farmi iznosila je 61.5%. Kompletno je sekvenciran genom 8 virusa koji su poticali sa sedam farmi. Na osnovu sekvenci HA i NA gena potvrđena je cirkulacija H1N1 i H3N2 podtipova. Na jednoj farmi ustanovlejna je cirkulacija oba podtipa, ali u različitim periodima ispitivanja. Na osnovu sekvence HA gena, sedam virusa H1 podtipa su grupisani u 1C.2.1 i 1A.3.3.2 genske grupe i H1N1pdm09 i evroazijsku “avian-like” H1avN1 linije. Na osnovu sekvence neuraminidaza gena ovih sedam H1N1 virusa su grupisani u evroazijsku “avian-like” H1avN1 liniju. Virus H3N2 podtipa na osnovu sekvenci HA i NA gena pripada liniji A/Swine/ Gent/1/1984 - “like” liniji.
PB  - Sciendo
T2  - Acta Veterinaria-Beograd
T1  - Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018
T1  - Filogenetska analiza HA i Na gena virusa influence svinja u Srbiji od 2016 do 2018 godine
VL  - 70
IS  - 1
SP  - 110
EP  - 125
DO  - 10.2478/acve-2020-0008
ER  - 

@article{
author = "Maksimović Zorić, Jelena and Milićević, Vesna and Stevančević, Ognjen and Chiapponi, Chiara and Potkonjak, Aleksandar and Stojanac, Nenad and Kureljušić, Branislav and Veljović, Lubiša and Radosavljević, Vladimir and Savić, Božidar",
year = "2020",
abstract = "Pigs are very important for the epidemiology of influenza A viruses, being commonly infected with the lineages of most adapted H1N1, H3N2, H1N2 swine subtypes. Epidemiological complexity of swine influenza is increasing by a periodic spillover of human or avian viruses in the pig population when genetic shifts can occur. The objectives of this research were to determine the presence of the influenza A virus in nasal and tracheobronchial swabs and lung tissue samples of ill and dead pigs on commercial farms, to determine circulating subtypes and characterize them through the phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes. A total of 255 samples collected from 13 farms were analyzed by means of real-time RT-PCR. The genome of influenza A virus was detected in 24 samples, which represented a 61.5% prevalence at the farms level (influenza A virus was confirmed in 8 out of 13 farms included in this study). Based on HA and NA gene sequences of 8 viruses, the circulation of H1N1 and H3N2 subtypes of influenza A viruses were determined. In addition, one farm exhibited a time separated circulation of H1N1 and H3N2 virus subtypes. Using Influenza Research Database, our viruses of the H1 subtype were classified into 1C.2.1 and 1A.3.3.2. clade. Based on the nucleotide sequences of HA genes, three viruses of the H1N1 subtype belong to the H1N1pdm09 lineage, and the other four to Eurasian “avian-like” H1avN1 lineage; while based on NA genes sequences, these seven viruses belong to Eurasian “avian-like” H1avN1 lineage. Both HA and NA genes of the virus of the H3N2 subtype belonged to the A/swine/ Gent/1/1984-like H3N2 lineage., Svinje su veoma važne u epidemiologiji infl uenca A virusa, jer je većina zapata širom sveta zaražena nekim od tri podtipa (H1N1, H1N2 ili H3N2). Pored toga, one su prijemčive i za ljudske i ptičije infl eunca A viruse, usled čega u njihovom organizmu može doći do genetskog reasortiranja i stvaranja genotipski i fenotipski novih virusa. Cilj ovog istraživanja je bio da se analizom nosnih i traeo-bronhijalnih briseva i pluća poreklom od bolesnih i ugilnulih svinja ispita prisustvo infl uenca A virusa, da se odrede cirkulišući podtipovi i fi logenetski okarakterišu kroz analizu sekvenci HA i NA gena. Tokom ovog istraživanja sa 13 farmi je sakupljeno i metodom realtime RT-PCR pregledano je 255 uzoraka. Genom infl uenca A virusa je utvrđen u 24 uzorka. Prevalencija virusa na nivou farmi iznosila je 61.5%. Kompletno je sekvenciran genom 8 virusa koji su poticali sa sedam farmi. Na osnovu sekvenci HA i NA gena potvrđena je cirkulacija H1N1 i H3N2 podtipova. Na jednoj farmi ustanovlejna je cirkulacija oba podtipa, ali u različitim periodima ispitivanja. Na osnovu sekvence HA gena, sedam virusa H1 podtipa su grupisani u 1C.2.1 i 1A.3.3.2 genske grupe i H1N1pdm09 i evroazijsku “avian-like” H1avN1 linije. Na osnovu sekvence neuraminidaza gena ovih sedam H1N1 virusa su grupisani u evroazijsku “avian-like” H1avN1 liniju. Virus H3N2 podtipa na osnovu sekvenci HA i NA gena pripada liniji A/Swine/ Gent/1/1984 - “like” liniji.",
publisher = "Sciendo",
journal = "Acta Veterinaria-Beograd",
title = "Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018, Filogenetska analiza HA i Na gena virusa influence svinja u Srbiji od 2016 do 2018 godine",
volume = "70",
number = "1",
pages = "110-125",
doi = "10.2478/acve-2020-0008"
}

Maksimović Zorić, J., Milićević, V., Stevančević, O., Chiapponi, C., Potkonjak, A., Stojanac, N., Kureljušić, B., Veljović, L., Radosavljević, V.,& Savić, B.. (2020). Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018. in Acta Veterinaria-Beograd
Sciendo., 70(1), 110-125.
https://doi.org/10.2478/acve-2020-0008

Maksimović Zorić J, Milićević V, Stevančević O, Chiapponi C, Potkonjak A, Stojanac N, Kureljušić B, Veljović L, Radosavljević V, Savić B. Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018. in Acta Veterinaria-Beograd. 2020;70(1):110-125.
doi:10.2478/acve-2020-0008 .

Maksimović Zorić, Jelena, Milićević, Vesna, Stevančević, Ognjen, Chiapponi, Chiara, Potkonjak, Aleksandar, Stojanac, Nenad, Kureljušić, Branislav, Veljović, Lubiša, Radosavljević, Vladimir, Savić, Božidar, "Phylogenetic analysis of HA and Na genes of swine influenza viruses in Serbia in 2016-2018" in Acta Veterinaria-Beograd, 70, no. 1 (2020):110-125,
https://doi.org/10.2478/acve-2020-0008 . .

TY  - JOUR
AU  - Pérez-Ramírez, Elisa
AU  - Cano-Gómez, Cristina
AU  - Llorente, Francisco
AU  - Adžić, Bojan
AU  - Al Ameer, Maisa
AU  - Djadjovski, Igor
AU  - Hage, Jeanne El
AU  - Mellouli, Fatiha El
AU  - Goletic, Teufik
AU  - Hovsepyan, Hermine
AU  - Karayel-Hacioglu, Ilke
AU  - Maksimović-Zorić, Jelena
AU  - Mejri, Selma
AU  - Sadaoui, Hassiba
AU  - Salem, Sayed Hassan
AU  - Sherifi, Kurtesh
AU  - Toklikishvili, Natela
AU  - Vodica, Ani
AU  - Monaco, Federica
AU  - Brun, Alejandro
AU  - Jiménez-Clavero, Miguel Ángel
AU  - Fernández-Pinero, Jovita
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1986
AB  - Rift Valley fever (RVF) is an arboviral zoonosis that primarily affects ruminants but can also cause illness in humans. The increasing impact of RVF in Africa and Middle East and the risk of expansion to other areas such as Europe, where competent mosquitos are already established, require the implementation of efficient surveillance programs in animal populations. For that, it is pivotal to regularly assess the performance of existing diagnostic tests and to evaluate the capacity of veterinary labs of endemic and non-endemic countries to detect the infection in an accurate and timely manner. In this context, the animal virology network of the MediLabSecure project organized between October 2016 and March 2017 an external quality assessment (EQA) to evaluate the RVF diagnostic capacities of beneficiary veterinary labs. This EQA was conceived as the last step of a training curriculum that included 2 diagnostic workshops that were organized by INIA-CISA (Spain) in 2015 and 2016. Seventeen veterinary diagnostic labs from 17 countries in the Mediterranean and Black Sea regions participated in this EQA. The exercise consisted of two panels of samples for molecular and serological detection of the virus. The laboratories were also provided with positive controls and all the kits and reagents necessary to perform the recommended diagnostic techniques. All the labs were able to apply the different protocols and to provide the results on time. The performance was good in the molecular panel with 70.6% of participants reporting 100% correct results, and excellent in the serological panel with 100% correct results reported by 94.1% of the labs. This EQA provided a good overview of the RVFV diagnostic capacities of the involved labs and demonstrated that most of them were able to correctly identify the virus genome and antibodies in different animal samples.
PB  - Public Library of Science
T2  - PLoS ONE
T1  - External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions
VL  - 15
DO  - 10.1371/journal.pone.0239478
ER  - 

@article{
author = "Pérez-Ramírez, Elisa and Cano-Gómez, Cristina and Llorente, Francisco and Adžić, Bojan and Al Ameer, Maisa and Djadjovski, Igor and Hage, Jeanne El and Mellouli, Fatiha El and Goletic, Teufik and Hovsepyan, Hermine and Karayel-Hacioglu, Ilke and Maksimović-Zorić, Jelena and Mejri, Selma and Sadaoui, Hassiba and Salem, Sayed Hassan and Sherifi, Kurtesh and Toklikishvili, Natela and Vodica, Ani and Monaco, Federica and Brun, Alejandro and Jiménez-Clavero, Miguel Ángel and Fernández-Pinero, Jovita",
year = "2020",
abstract = "Rift Valley fever (RVF) is an arboviral zoonosis that primarily affects ruminants but can also cause illness in humans. The increasing impact of RVF in Africa and Middle East and the risk of expansion to other areas such as Europe, where competent mosquitos are already established, require the implementation of efficient surveillance programs in animal populations. For that, it is pivotal to regularly assess the performance of existing diagnostic tests and to evaluate the capacity of veterinary labs of endemic and non-endemic countries to detect the infection in an accurate and timely manner. In this context, the animal virology network of the MediLabSecure project organized between October 2016 and March 2017 an external quality assessment (EQA) to evaluate the RVF diagnostic capacities of beneficiary veterinary labs. This EQA was conceived as the last step of a training curriculum that included 2 diagnostic workshops that were organized by INIA-CISA (Spain) in 2015 and 2016. Seventeen veterinary diagnostic labs from 17 countries in the Mediterranean and Black Sea regions participated in this EQA. The exercise consisted of two panels of samples for molecular and serological detection of the virus. The laboratories were also provided with positive controls and all the kits and reagents necessary to perform the recommended diagnostic techniques. All the labs were able to apply the different protocols and to provide the results on time. The performance was good in the molecular panel with 70.6% of participants reporting 100% correct results, and excellent in the serological panel with 100% correct results reported by 94.1% of the labs. This EQA provided a good overview of the RVFV diagnostic capacities of the involved labs and demonstrated that most of them were able to correctly identify the virus genome and antibodies in different animal samples.",
publisher = "Public Library of Science",
journal = "PLoS ONE",
title = "External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions",
volume = "15",
doi = "10.1371/journal.pone.0239478"
}

Pérez-Ramírez, E., Cano-Gómez, C., Llorente, F., Adžić, B., Al Ameer, M., Djadjovski, I., Hage, J. E., Mellouli, F. E., Goletic, T., Hovsepyan, H., Karayel-Hacioglu, I., Maksimović-Zorić, J., Mejri, S., Sadaoui, H., Salem, S. H., Sherifi, K., Toklikishvili, N., Vodica, A., Monaco, F., Brun, A., Jiménez-Clavero, M. Á.,& Fernández-Pinero, J.. (2020). External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions. in PLoS ONE
Public Library of Science., 15.
https://doi.org/10.1371/journal.pone.0239478

Pérez-Ramírez E, Cano-Gómez C, Llorente F, Adžić B, Al Ameer M, Djadjovski I, Hage JE, Mellouli FE, Goletic T, Hovsepyan H, Karayel-Hacioglu I, Maksimović-Zorić J, Mejri S, Sadaoui H, Salem SH, Sherifi K, Toklikishvili N, Vodica A, Monaco F, Brun A, Jiménez-Clavero MÁ, Fernández-Pinero J. External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions. in PLoS ONE. 2020;15.
doi:10.1371/journal.pone.0239478 .

Pérez-Ramírez, Elisa, Cano-Gómez, Cristina, Llorente, Francisco, Adžić, Bojan, Al Ameer, Maisa, Djadjovski, Igor, Hage, Jeanne El, Mellouli, Fatiha El, Goletic, Teufik, Hovsepyan, Hermine, Karayel-Hacioglu, Ilke, Maksimović-Zorić, Jelena, Mejri, Selma, Sadaoui, Hassiba, Salem, Sayed Hassan, Sherifi, Kurtesh, Toklikishvili, Natela, Vodica, Ani, Monaco, Federica, Brun, Alejandro, Jiménez-Clavero, Miguel Ángel, Fernández-Pinero, Jovita, "External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions" in PLoS ONE, 15 (2020),
https://doi.org/10.1371/journal.pone.0239478 . .

TY  - JOUR
AU  - Pavlović, Ivan
AU  - Veljović, Ljubiša
AU  - Milićević, Vesna
AU  - Maksimović-Zorić, Jelena
AU  - Stanojević, Slobodan
AU  - Radanović, Oliver
AU  - Đurić, Boban
AU  - Plavšić, Budimir
AU  - Vasić, Ana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1958
AB  - Genus Culicoides spp. includes small insects 0.5-2 mm in length, usually grey or black and at first glance very similar to mosquitoes. They are strictly hematophagous, feeding by attacking hosts outdoors and indoors (although they are less susceptible to the stationing like Aedes mosquitoes do). The sting is very painful at the injection site and often followed by hypersensibilisation with the consequent formation of allergic dermatitis. In addition, the insects carry and transmit a multitude of diseases, often of a zoonotic character, and therefore are of great epidemiological importance. In our country, continuous monitoring of Culicolides spp. has been carried out and seasonal dynamics of their appearance in the period 2015-2016 is presented in this article. During October 2015, the presence of Culicolides spp. was confirmed in 10.00% of samples; in November, their presence was not established, whereas in December, 2.35% of samples proved positive for the presence of Culicolides spp. During 2016, from January to March, no Culicolides spp. were found in any of the examined samples. During April, their prevalence was 9.63%, in May - 6.74%, in June - 3.70%, in July - 15.78%, in August - 18.07%, in September - 27.27%, and in October - 45.65%. In Serbia, the dominant Culicoides spp. species are Obsoletus complex and Pulicaris complex established in 57.21% and 33.37% of samples, respectively. Other species are present in lesser extent. In Obsoletus complex, the dominant species was Culicoides obsoletus/scoticus. The percentage of Culicoides obsoletus /scoticus males in samples was 25.52%. Non-pigmented (young) females were present in 66.06% of samples; females who took blood in 7.55% and 0.87% were gravid females. In Pulicaris complex, the dominant species was Culicoides pulicaris. Males of Culicoides pulicaris were found in 19.23% of samples, non-pigmented (young) females in 70.96%, females who took blood in 9.08% while 0.73% were gravid females.
AB  - Rod Culicoides spp. su mali insekti veličine 0,5-2 mm, većinom sivkaste ili  crne  boje  i  na  prvi  pogled  veoma  slični  komarcima. Oni  su  striktne  hematofage. Ubod  je  veoma  bolan,  a  mesta  uboda  su  često  hipersenzibilisana  uz  posledično  nastajanje  alergijskih  dermatitisa. Uz  to  oni  prenose  mnoštvo  oboljenja  često  zoonotskog  karaktera  tako  da  su  od  izuzetnog  epidemiološkog  značaja. U  našoj  zemlji  se  vrši  stalni  monitoring  Culicolides-a  i  ovde  dajemo  prikaz  sezonske  dinamike  njihovog  pojavljivanja  u  periodu  2015-2016.godine. Tokom  2015.  godine  u  oktobru  je  njihovo  prisustvo ustanovljeno u 10.,00% uzoraka, u novembru ih nije bilo, a tokom decembra su bili prisutni u 2,35%. Tokom 2016. godine tokom januara, februara  i  marta  nisu  nađeni  ni  u  jednom  prispelom  uzorku. Tokom  aprila  meseca njihova prevalencija je iznosila 9,63%, u maju je bila 6,74%, u junu 3,70%, u julu 15,78%, u avgustu 18,07%, u septembru 27,27% i u oktobru 45,65%. U  Srbiji  su  dominantne  vrste  Culicoides spp.  iz  Obsoletus  kompleksa  i  ustanovljeni  su  u  57,21%.  Culicoides spp.  iz  Pulicaris  kompleksa  ustanovljeni  su  33,37%  dok  su  ostale  vrste  manje  zastupljene.  Kod  Culicoides  spp.  iz  Obsoletus  kompleksa  dominantna  vrsta  je  bila  Culicoides obsoletus/scoticus.  Mužjaci  su  nađeni  u  25,52%,  nepigmentisane  (mlade)  ženke u 66,06%, ženke koje su uzele krv u 7,55%, a 0,87% su bile gravidne ženke. Kod Culicoides spp. iz Pulicaris kompleksa dominantna vrsta je bila Culicoides pulicaris. Mužjaci su nađeni u 19,23%, nepigmentisane (mlade) ženke u 70,96%, ženke koje su uzele krv u 9,08%, a 0,73% su bile gravidne ženke.
PB  - Scientific Veterinary Institute “Novi Sad”, Novi Sad
T2  - Archives of Veterinary Medicine
T1  - Seasonal dynamics of the presence of culicoides spp. in Serbia in the period 2015-2016
T1  - Sezonska dinamika prisustva culicoides spp u Srbiji u periodu 2015-2016. godine
VL  - 10
IS  - 1
DO  - 10.46784/e-avm.v10i1.76
ER  - 

@article{
author = "Pavlović, Ivan and Veljović, Ljubiša and Milićević, Vesna and Maksimović-Zorić, Jelena and Stanojević, Slobodan and Radanović, Oliver and Đurić, Boban and Plavšić, Budimir and Vasić, Ana",
year = "2019",
abstract = "Genus Culicoides spp. includes small insects 0.5-2 mm in length, usually grey or black and at first glance very similar to mosquitoes. They are strictly hematophagous, feeding by attacking hosts outdoors and indoors (although they are less susceptible to the stationing like Aedes mosquitoes do). The sting is very painful at the injection site and often followed by hypersensibilisation with the consequent formation of allergic dermatitis. In addition, the insects carry and transmit a multitude of diseases, often of a zoonotic character, and therefore are of great epidemiological importance. In our country, continuous monitoring of Culicolides spp. has been carried out and seasonal dynamics of their appearance in the period 2015-2016 is presented in this article. During October 2015, the presence of Culicolides spp. was confirmed in 10.00% of samples; in November, their presence was not established, whereas in December, 2.35% of samples proved positive for the presence of Culicolides spp. During 2016, from January to March, no Culicolides spp. were found in any of the examined samples. During April, their prevalence was 9.63%, in May - 6.74%, in June - 3.70%, in July - 15.78%, in August - 18.07%, in September - 27.27%, and in October - 45.65%. In Serbia, the dominant Culicoides spp. species are Obsoletus complex and Pulicaris complex established in 57.21% and 33.37% of samples, respectively. Other species are present in lesser extent. In Obsoletus complex, the dominant species was Culicoides obsoletus/scoticus. The percentage of Culicoides obsoletus /scoticus males in samples was 25.52%. Non-pigmented (young) females were present in 66.06% of samples; females who took blood in 7.55% and 0.87% were gravid females. In Pulicaris complex, the dominant species was Culicoides pulicaris. Males of Culicoides pulicaris were found in 19.23% of samples, non-pigmented (young) females in 70.96%, females who took blood in 9.08% while 0.73% were gravid females., Rod Culicoides spp. su mali insekti veličine 0,5-2 mm, većinom sivkaste ili  crne  boje  i  na  prvi  pogled  veoma  slični  komarcima. Oni  su  striktne  hematofage. Ubod  je  veoma  bolan,  a  mesta  uboda  su  često  hipersenzibilisana  uz  posledično  nastajanje  alergijskih  dermatitisa. Uz  to  oni  prenose  mnoštvo  oboljenja  često  zoonotskog  karaktera  tako  da  su  od  izuzetnog  epidemiološkog  značaja. U  našoj  zemlji  se  vrši  stalni  monitoring  Culicolides-a  i  ovde  dajemo  prikaz  sezonske  dinamike  njihovog  pojavljivanja  u  periodu  2015-2016.godine. Tokom  2015.  godine  u  oktobru  je  njihovo  prisustvo ustanovljeno u 10.,00% uzoraka, u novembru ih nije bilo, a tokom decembra su bili prisutni u 2,35%. Tokom 2016. godine tokom januara, februara  i  marta  nisu  nađeni  ni  u  jednom  prispelom  uzorku. Tokom  aprila  meseca njihova prevalencija je iznosila 9,63%, u maju je bila 6,74%, u junu 3,70%, u julu 15,78%, u avgustu 18,07%, u septembru 27,27% i u oktobru 45,65%. U  Srbiji  su  dominantne  vrste  Culicoides spp.  iz  Obsoletus  kompleksa  i  ustanovljeni  su  u  57,21%.  Culicoides spp.  iz  Pulicaris  kompleksa  ustanovljeni  su  33,37%  dok  su  ostale  vrste  manje  zastupljene.  Kod  Culicoides  spp.  iz  Obsoletus  kompleksa  dominantna  vrsta  je  bila  Culicoides obsoletus/scoticus.  Mužjaci  su  nađeni  u  25,52%,  nepigmentisane  (mlade)  ženke u 66,06%, ženke koje su uzele krv u 7,55%, a 0,87% su bile gravidne ženke. Kod Culicoides spp. iz Pulicaris kompleksa dominantna vrsta je bila Culicoides pulicaris. Mužjaci su nađeni u 19,23%, nepigmentisane (mlade) ženke u 70,96%, ženke koje su uzele krv u 9,08%, a 0,73% su bile gravidne ženke.",
publisher = "Scientific Veterinary Institute “Novi Sad”, Novi Sad",
journal = "Archives of Veterinary Medicine",
title = "Seasonal dynamics of the presence of culicoides spp. in Serbia in the period 2015-2016, Sezonska dinamika prisustva culicoides spp u Srbiji u periodu 2015-2016. godine",
volume = "10",
number = "1",
doi = "10.46784/e-avm.v10i1.76"
}

Pavlović, I., Veljović, L., Milićević, V., Maksimović-Zorić, J., Stanojević, S., Radanović, O., Đurić, B., Plavšić, B.,& Vasić, A.. (2019). Seasonal dynamics of the presence of culicoides spp. in Serbia in the period 2015-2016. in Archives of Veterinary Medicine
Scientific Veterinary Institute “Novi Sad”, Novi Sad., 10(1).
https://doi.org/10.46784/e-avm.v10i1.76

Pavlović I, Veljović L, Milićević V, Maksimović-Zorić J, Stanojević S, Radanović O, Đurić B, Plavšić B, Vasić A. Seasonal dynamics of the presence of culicoides spp. in Serbia in the period 2015-2016. in Archives of Veterinary Medicine. 2019;10(1).
doi:10.46784/e-avm.v10i1.76 .

Pavlović, Ivan, Veljović, Ljubiša, Milićević, Vesna, Maksimović-Zorić, Jelena, Stanojević, Slobodan, Radanović, Oliver, Đurić, Boban, Plavšić, Budimir, Vasić, Ana, "Seasonal dynamics of the presence of culicoides spp. in Serbia in the period 2015-2016" in Archives of Veterinary Medicine, 10, no. 1 (2019),
https://doi.org/10.46784/e-avm.v10i1.76 . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Kureljušić, Jasna
AU  - Šekler, Milanko
AU  - Nešić, Ksenija
AU  - Veljović, Ljubiša
AU  - Zorić-Maksimović, Jelena
AU  - Radosavljević, Vladimir
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1657
AB  - Considering the intensive trading nowadays, the honey from the local market was tested for the presence of the six most common bee viruses. To prove the suitability of honey as a sample for the bee viruses detection, the set of different sample types taken directly from the hives we comparatively tested. The study included 30 samples of domestic and 5 samples of imported honey. Additionally, we tested 40 sets of samples including live bees, dead bees, and the honey taken from four apiaries for the evaluation of honey suitability for the virus detection, Two out of the six most common bee viruses were detected in the samples of honey from the market. Black queen cell virus (BQCV) genome was found in 24 domestic honey samples and Kashmir bee virus (KBV) genome was detected in one sample of imported honey. The nucleotide sequences of 24 BQCV isolates showed the highest identity (86.4%) with strains from Europe at the polyprotein gene, whilst the Serbian isolates between each other showed 98.5% similarity. By comparative testing of the different type of samples, in three out of four apiaries BQCV genome was detected in both bees and honey. Evaluating the suitability of honey for the detection of the viral disease by simultaneous testing of live, dead bees, and honey from the same hive, it was shown that the honey can be successfully used for the detection of BQCV. Since, as of yet, there has been no evidence of KBV circulation in Serbia, after its detection in imported honey, there is a substantial risk of its introduction and consequently the need for its surveillance. Therefore, the programs of bee diseases screening should be included in the regular control procedures for the international trade. In addition to this benefit, honey gives an opportunity to beekeepers for continuous monitoring of bees health status.
PB  - Springeropen, London
T2  - AMB EXPRESS
T1  - Molecular detection of black queen cell virus and Kashmir bee virus in honey
VL  - 8
SP  - 128
DO  - 10.1186/s13568-018-0655-7
ER  - 

@article{
author = "Milićević, Vesna and Radojičić, Sonja and Kureljušić, Jasna and Šekler, Milanko and Nešić, Ksenija and Veljović, Ljubiša and Zorić-Maksimović, Jelena and Radosavljević, Vladimir",
year = "2018",
abstract = "Considering the intensive trading nowadays, the honey from the local market was tested for the presence of the six most common bee viruses. To prove the suitability of honey as a sample for the bee viruses detection, the set of different sample types taken directly from the hives we comparatively tested. The study included 30 samples of domestic and 5 samples of imported honey. Additionally, we tested 40 sets of samples including live bees, dead bees, and the honey taken from four apiaries for the evaluation of honey suitability for the virus detection, Two out of the six most common bee viruses were detected in the samples of honey from the market. Black queen cell virus (BQCV) genome was found in 24 domestic honey samples and Kashmir bee virus (KBV) genome was detected in one sample of imported honey. The nucleotide sequences of 24 BQCV isolates showed the highest identity (86.4%) with strains from Europe at the polyprotein gene, whilst the Serbian isolates between each other showed 98.5% similarity. By comparative testing of the different type of samples, in three out of four apiaries BQCV genome was detected in both bees and honey. Evaluating the suitability of honey for the detection of the viral disease by simultaneous testing of live, dead bees, and honey from the same hive, it was shown that the honey can be successfully used for the detection of BQCV. Since, as of yet, there has been no evidence of KBV circulation in Serbia, after its detection in imported honey, there is a substantial risk of its introduction and consequently the need for its surveillance. Therefore, the programs of bee diseases screening should be included in the regular control procedures for the international trade. In addition to this benefit, honey gives an opportunity to beekeepers for continuous monitoring of bees health status.",
publisher = "Springeropen, London",
journal = "AMB EXPRESS",
title = "Molecular detection of black queen cell virus and Kashmir bee virus in honey",
volume = "8",
pages = "128",
doi = "10.1186/s13568-018-0655-7"
}

Milićević, V., Radojičić, S., Kureljušić, J., Šekler, M., Nešić, K., Veljović, L., Zorić-Maksimović, J.,& Radosavljević, V.. (2018). Molecular detection of black queen cell virus and Kashmir bee virus in honey. in AMB EXPRESS
Springeropen, London., 8, 128.
https://doi.org/10.1186/s13568-018-0655-7

Milićević V, Radojičić S, Kureljušić J, Šekler M, Nešić K, Veljović L, Zorić-Maksimović J, Radosavljević V. Molecular detection of black queen cell virus and Kashmir bee virus in honey. in AMB EXPRESS. 2018;8:128.
doi:10.1186/s13568-018-0655-7 .

Milićević, Vesna, Radojičić, Sonja, Kureljušić, Jasna, Šekler, Milanko, Nešić, Ksenija, Veljović, Ljubiša, Zorić-Maksimović, Jelena, Radosavljević, Vladimir, "Molecular detection of black queen cell virus and Kashmir bee virus in honey" in AMB EXPRESS, 8 (2018):128,
https://doi.org/10.1186/s13568-018-0655-7 . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Maksimović-Zorić, Jelena
AU  - Žutić, Jadranka
AU  - Kureljušić, Branislav
AU  - Radosavljević, Vladimir
AU  - Jezdimirović, Nemanja
AU  - Radojičić, Sonja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1564
AB  - Vaccines are a very effective tool for the prevention and eradication of infective diseases in both veterinary and human medicine. Although for safety reasons, vaccines undergo very strict controls before being placed on the market, the risk of adverse reactions is not eliminated. According to the World Health Organization (WHO), adverse event following immunization (AEFI) is any untoward medical occurrence that follows immunization and which does not necessarily have a causal relationship with the usage of the vaccine. Adverse reactions may arise as a direct consequence of immunization due to the specificity of the vaccine itself, the quality of the vaccine or the immunization errors. In addition, adverse reactions may also be the result of a coincidental relationship between the effect and immunization. However, biological mechanisms of AEFI are very complex. During the mass vaccination campaigns, when a large number of animals are vaccinated in a short period of time, adverse reactions are expected to be the most frequently reported. In Serbia, livestock is currently being vaccinated against Lumpy Skin Disease, Bluetongue, and Classical swine fever.
AB  - Vakcine su veoma efikasan alat za prevenciju i iskorenjivanje infektivnih bolesti u veterinarskoj i humanoj medicini. Iako iz sigurnosnih razloga vakcine prolaze kroz vrlo stroge kontrole pre stavljanja u promet, rizik od neželjenih reakcija nije eliminisan. Prema Svetskoj zdravstvenoj organizaciji (SZO), neželjeni događaj nakon imunizacije je svaka neugodna medicinska pojava koja prati imunizaciju i koja ne mora nužno imati uzročnu vezu sa upotrebom vakcine. Neželjene reakcije mogu nastati kao direktna posledica imunizacije usled specifičnosti same vakcine, kvaliteta vakcine ili grešaka prilikom imunizacije. Pored toga, neželjene reakcije mogu biti i posledica slučajnog odnosa između nastalog efekta i imunizacije. Međutim, biološki mehanizmi nastanka ovih reakcija su veoma složeni. Tokom kampanja mas-ovnih vakcinacija, kada se veliki broj životinja vakciniše u kratkom vremen-skom periodu, očekuje se da se neželjene reakcije najčešće prijavljuju. U Srbiji se stoka trenutno vakciniše protiv bolesti kvrgave kože, bolesti plavog jezika i klasične svinjske kuge. Pošto nijedna od ove tri vakcine nije DIVA, praćenje neželjenih događaja koji se mogu pojaviti je od izuzetnog značaja.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Some adverse events following immunization in veterinary medicine
T1  - Procena neželjenih reakcija nakon vakcinacije u veterini
VL  - 11
IS  - 1
SP  - 67
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1564
ER  - 

@article{
author = "Milićević, Vesna and Veljović, Ljubiša and Maksimović-Zorić, Jelena and Žutić, Jadranka and Kureljušić, Branislav and Radosavljević, Vladimir and Jezdimirović, Nemanja and Radojičić, Sonja",
year = "2018",
abstract = "Vaccines are a very effective tool for the prevention and eradication of infective diseases in both veterinary and human medicine. Although for safety reasons, vaccines undergo very strict controls before being placed on the market, the risk of adverse reactions is not eliminated. According to the World Health Organization (WHO), adverse event following immunization (AEFI) is any untoward medical occurrence that follows immunization and which does not necessarily have a causal relationship with the usage of the vaccine. Adverse reactions may arise as a direct consequence of immunization due to the specificity of the vaccine itself, the quality of the vaccine or the immunization errors. In addition, adverse reactions may also be the result of a coincidental relationship between the effect and immunization. However, biological mechanisms of AEFI are very complex. During the mass vaccination campaigns, when a large number of animals are vaccinated in a short period of time, adverse reactions are expected to be the most frequently reported. In Serbia, livestock is currently being vaccinated against Lumpy Skin Disease, Bluetongue, and Classical swine fever., Vakcine su veoma efikasan alat za prevenciju i iskorenjivanje infektivnih bolesti u veterinarskoj i humanoj medicini. Iako iz sigurnosnih razloga vakcine prolaze kroz vrlo stroge kontrole pre stavljanja u promet, rizik od neželjenih reakcija nije eliminisan. Prema Svetskoj zdravstvenoj organizaciji (SZO), neželjeni događaj nakon imunizacije je svaka neugodna medicinska pojava koja prati imunizaciju i koja ne mora nužno imati uzročnu vezu sa upotrebom vakcine. Neželjene reakcije mogu nastati kao direktna posledica imunizacije usled specifičnosti same vakcine, kvaliteta vakcine ili grešaka prilikom imunizacije. Pored toga, neželjene reakcije mogu biti i posledica slučajnog odnosa između nastalog efekta i imunizacije. Međutim, biološki mehanizmi nastanka ovih reakcija su veoma složeni. Tokom kampanja mas-ovnih vakcinacija, kada se veliki broj životinja vakciniše u kratkom vremen-skom periodu, očekuje se da se neželjene reakcije najčešće prijavljuju. U Srbiji se stoka trenutno vakciniše protiv bolesti kvrgave kože, bolesti plavog jezika i klasične svinjske kuge. Pošto nijedna od ove tri vakcine nije DIVA, praćenje neželjenih događaja koji se mogu pojaviti je od izuzetnog značaja.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Some adverse events following immunization in veterinary medicine, Procena neželjenih reakcija nakon vakcinacije u veterini",
volume = "11",
number = "1",
pages = "67-77",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1564"
}

Milićević, V., Veljović, L., Maksimović-Zorić, J., Žutić, J., Kureljušić, B., Radosavljević, V., Jezdimirović, N.,& Radojičić, S.. (2018). Some adverse events following immunization in veterinary medicine. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 11(1), 67-77.
https://hdl.handle.net/21.15107/rcub_veterinar_1564

Milićević V, Veljović L, Maksimović-Zorić J, Žutić J, Kureljušić B, Radosavljević V, Jezdimirović N, Radojičić S. Some adverse events following immunization in veterinary medicine. in Arhiv veterinarske medicine. 2018;11(1):67-77.
https://hdl.handle.net/21.15107/rcub_veterinar_1564 .

Milićević, Vesna, Veljović, Ljubiša, Maksimović-Zorić, Jelena, Žutić, Jadranka, Kureljušić, Branislav, Radosavljević, Vladimir, Jezdimirović, Nemanja, Radojičić, Sonja, "Some adverse events following immunization in veterinary medicine" in Arhiv veterinarske medicine, 11, no. 1 (2018):67-77,
https://hdl.handle.net/21.15107/rcub_veterinar_1564 .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Radosavljević, Vladimir
AU  - Veljović, Ljubiša
AU  - Maksimović-Zorić, Jelena
AU  - Radojičić, Sonja
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1450
AB  - African swine fever (ASF) is a viral disease of domestic pigs and wild boar. Due to the very serious socioeconomic consequences, the disease is one of the most important ones nowadays. African swine fever is an enzootic disease in many countries in Sub-Saharan Africa, in Sardinia, and Trans Caucasus countries. After its occurrence in Georgia in 2007, ASF spread to Armenia and Russian Federation, and in 2008. to Azerbaijan. Since then, its progressive moving toward the west has been recorded. Despite the number of undertaken preventive and control measures in the European Union (EU), ASF has been still spreading. During 2017, the disease has been reported in domestic pigs in Estonia, Italy-Sardinia, Latvia, Lithuania, Poland, Romania, and Ukraine. ASF cases in domestic pigs have also been reported in Moldova in 2017. The number of diagnosed cases in wild boar in 2017 is much higher than in domestic pigs. ASF outbreak in wild boar in the Czech Republic well describes the possible viral 'jump' into a new region. The source of infection hasn't been confirmed yet, but it is common that such leaps are due to either swill feeding or improperly disposal of food rather than to the animal movements. Since the lack of effective vaccine makes eradication even more difficult, the prevention of viral entry into the new areas is of the most importance. With the same aim, since 2011.the surveillance of ASF has been implemented in Serbia.
AB  - Afrička kuga svinja (AKS) je virusna bolest domaćih i divljih svinja. Socioekonomske posledice ove bolesti svrstavaju je u najznačajnije bolesti današnjice. Afrička kuga svinja je enzootska bolest u mnogim zemljama južno od Sahare, na Sardiniji i Kavkazu. Pošto se pojavila 2007. godine u Gruziji, AKS se iste godine proširila na Jermeniju i Rusiju, a 2008. na Azerbejdžan. Od tada se beleži progresivno kretanje virusa ka zapadu. Uprkos svim preventivnim i kontrolnim merama koje se sprovode u Evropskoj uniji (EU), afrička kuga svinja se i dalje širi. Tokom 2017. godine kod domaćih svinja je dokazana u Estoniji, Italiji - Sardinija, Letoniji, Litvaniji, Poljskoj, Rumuniji i Ukrajini. Slučajevi AKS kod domaćih svinja u Moldaviji su takođe registrovani i u 2017. godini. Broj dijagnostikovanih slučajeva kod divljih svinja u 2017. je značajno veći u odnosu na broj slučajeva kod domaćih. Pojava AKS u Češkoj 2017. godine kod divljih svinja predstavlja veliki "skok" virusa u novo područje. Izvor infekcije još uvek nije potvrđen, ali je uobičajeno da se ovakve pojave dešavaju kao posledica hranjenja životinja ostacima hrane, a ne zbog kretanja životinja. Budući da je iskorenjivanje AKS veoma otežano u odsustvu efikasne vakcine, prevencija unosa virusa u nova područja je od najvećeg značaja. Sa tim ciljem, u Srbiji se od 2011. godine sprovodi nadzor kod divljih svinja na afričku kugu.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Epizootiological situation of African swine fever in Europe
T1  - Epizootiološka situacija afričke kuge svinja u Evropi
VL  - 10
IS  - 2
SP  - 13
EP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1450
ER  - 

@article{
author = "Milićević, Vesna and Radosavljević, Vladimir and Veljović, Ljubiša and Maksimović-Zorić, Jelena and Radojičić, Sonja",
year = "2017",
abstract = "African swine fever (ASF) is a viral disease of domestic pigs and wild boar. Due to the very serious socioeconomic consequences, the disease is one of the most important ones nowadays. African swine fever is an enzootic disease in many countries in Sub-Saharan Africa, in Sardinia, and Trans Caucasus countries. After its occurrence in Georgia in 2007, ASF spread to Armenia and Russian Federation, and in 2008. to Azerbaijan. Since then, its progressive moving toward the west has been recorded. Despite the number of undertaken preventive and control measures in the European Union (EU), ASF has been still spreading. During 2017, the disease has been reported in domestic pigs in Estonia, Italy-Sardinia, Latvia, Lithuania, Poland, Romania, and Ukraine. ASF cases in domestic pigs have also been reported in Moldova in 2017. The number of diagnosed cases in wild boar in 2017 is much higher than in domestic pigs. ASF outbreak in wild boar in the Czech Republic well describes the possible viral 'jump' into a new region. The source of infection hasn't been confirmed yet, but it is common that such leaps are due to either swill feeding or improperly disposal of food rather than to the animal movements. Since the lack of effective vaccine makes eradication even more difficult, the prevention of viral entry into the new areas is of the most importance. With the same aim, since 2011.the surveillance of ASF has been implemented in Serbia., Afrička kuga svinja (AKS) je virusna bolest domaćih i divljih svinja. Socioekonomske posledice ove bolesti svrstavaju je u najznačajnije bolesti današnjice. Afrička kuga svinja je enzootska bolest u mnogim zemljama južno od Sahare, na Sardiniji i Kavkazu. Pošto se pojavila 2007. godine u Gruziji, AKS se iste godine proširila na Jermeniju i Rusiju, a 2008. na Azerbejdžan. Od tada se beleži progresivno kretanje virusa ka zapadu. Uprkos svim preventivnim i kontrolnim merama koje se sprovode u Evropskoj uniji (EU), afrička kuga svinja se i dalje širi. Tokom 2017. godine kod domaćih svinja je dokazana u Estoniji, Italiji - Sardinija, Letoniji, Litvaniji, Poljskoj, Rumuniji i Ukrajini. Slučajevi AKS kod domaćih svinja u Moldaviji su takođe registrovani i u 2017. godini. Broj dijagnostikovanih slučajeva kod divljih svinja u 2017. je značajno veći u odnosu na broj slučajeva kod domaćih. Pojava AKS u Češkoj 2017. godine kod divljih svinja predstavlja veliki "skok" virusa u novo područje. Izvor infekcije još uvek nije potvrđen, ali je uobičajeno da se ovakve pojave dešavaju kao posledica hranjenja životinja ostacima hrane, a ne zbog kretanja životinja. Budući da je iskorenjivanje AKS veoma otežano u odsustvu efikasne vakcine, prevencija unosa virusa u nova područja je od najvećeg značaja. Sa tim ciljem, u Srbiji se od 2011. godine sprovodi nadzor kod divljih svinja na afričku kugu.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Epizootiological situation of African swine fever in Europe, Epizootiološka situacija afričke kuge svinja u Evropi",
volume = "10",
number = "2",
pages = "13-22",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1450"
}

Milićević, V., Radosavljević, V., Veljović, L., Maksimović-Zorić, J.,& Radojičić, S.. (2017). Epizootiological situation of African swine fever in Europe. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 10(2), 13-22.
https://hdl.handle.net/21.15107/rcub_veterinar_1450

Milićević V, Radosavljević V, Veljović L, Maksimović-Zorić J, Radojičić S. Epizootiological situation of African swine fever in Europe. in Arhiv veterinarske medicine. 2017;10(2):13-22.
https://hdl.handle.net/21.15107/rcub_veterinar_1450 .

Milićević, Vesna, Radosavljević, Vladimir, Veljović, Ljubiša, Maksimović-Zorić, Jelena, Radojičić, Sonja, "Epizootiological situation of African swine fever in Europe" in Arhiv veterinarske medicine, 10, no. 2 (2017):13-22,
https://hdl.handle.net/21.15107/rcub_veterinar_1450 .

TY  - JOUR
AU  - Veljović, Ljubiša
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Miković, Radoš
AU  - Zorić, Andrea
AU  - Marković, Maja
AU  - Milićević, Vesna
AU  - Stamenković, Miodrag
AU  - Stanojević, Maja
AU  - Maksimović-Zorić, Jelena
AU  - Petrović, Tamaš
AU  - Nišavić, Jakov
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1368
AB  - The presence of bovine parainfluenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight samples (8/119; 6.7%). The confirmation of isolated strains of BPIV3 was done by the virus-neutralization test. In addition, all samples of bovine nasal swabs were also examined for the presence of BPIV3 virus using RT-PCR with primers specific for the part of HN gene. The presence of BPIV3 was detected in eight samples (8/119; 6.7%) that were also positive upon virus isolation. The molecular characterization based on nucleotide sequencing of the part of the HN gene showed that all BPIV3 isolates belonged to genotype C of BPIV3. They branched in one distinct cluster with three different branches, but these branches were very similar to each other (98.1% to 99.8%). Serbian BPIV3c isolates were most similar to the Chinese BPIV3c isolates SD0805, SD0809 and SD0835 (from 97.92% to 99.7%), and to South Korean (12Q061), Japanese (HS9) and American (TVMDL16 and TVMDL20) BPIV3c strains (from 97.1% to 98.8%), and distinct from American (TVMDL15and TVMDL17) and Australian (Q5592) BPI3V genotype B strains (only 79.9% to 82.3% similarity), as well as from the genotype A BPIV3 strains from different countries published in GenBank.
AB  - Ukupno je ispitano 119 uzoraka nosnih briseva goveda na prisustvo parainfluenca 3 virusa goveda (bovine parainfluenza virus type, eng. - 3 BPIV3). Iz svih uzoraka nosnih briseva je vršena izolacija virusa na ćelijskoj liniji MDBK. Pojava citopatogenog efekta na kulturi ćelija, nakon 48h, odnosno 72h, utvrđena je kod osam uzoraka nosnih briseva (8/119; 6.7%). Identifikacija izolovanih sojeva BPIV3 je izvršena primenom virus neutralizacionog testa. Dodatno, svi uzorci nosnih briseva goveda su ispitani na prisustvo BPIV3 i primenom metode RT-PCR uz korišćenje prajmera specifičnih za deo HN gena virusa. Prisustvo virusne nukleinske kiseline je utvrđeno kod osam uzoraka nosnih briseva (8/119; 6.7%), koji su bili pozitivni i na izolaciji virusa. Molekularna karakterizacija zasnovana na sekvenciranju dela HN gena izolata BPIV3 iz Srbije je potvrdila da svi pripadaju genotipu C BPIV3 (BPIV3c). Oni su se u filogenetskom stablu granali u tri različite grane koje su međusobno veoma slične (98.1% do 99.8%). Izolati BPIV3 iz Srbije su pokazali visok stepen sličnosti nukleotidnih sekvenci sa BPIV3c sojevima SD0805, SD0809 i SD0835 iz Kine (97.92% do 99.7%), odnosno sa BPIV3c sojevima 12Q061 iz Južne Koreje, HS9 iz Japana i TVMDL16 i TVMDL20 iz Amerike (sličnost od 97.1% do 98.8%), kao i različitosti u odnosu na nukleotidne sekvence sojeva TVMDL15 i TVMDL17 izolovanih u Americi i soja Q5592 izolovanog u Australiji, a koji su pripadali genotipu B BPIV3 (sličnost od 79.9% do 82.3%). Slična razlika je utvrđena i sa nukleotidnim sekvencama sojeva virusa, poreklom iz različitih država, svrstanih u genotip A BPIV3, a objavljenih u genskoj bazi podataka (NCBI GenBank).
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia
T1  - Izolacija i molekularna detekcija parainfluenca 3 virusa kod goveda u Srbiji
VL  - 66
IS  - 4
SP  - 509
EP  - 519
DO  - 10.1515/acve-2016-0044
ER  - 

@article{
author = "Veljović, Ljubiša and Knežević, Aleksandra and Milić, Nenad and Krnjaić, Dejan and Miković, Radoš and Zorić, Andrea and Marković, Maja and Milićević, Vesna and Stamenković, Miodrag and Stanojević, Maja and Maksimović-Zorić, Jelena and Petrović, Tamaš and Nišavić, Jakov",
year = "2016",
abstract = "The presence of bovine parainfluenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight samples (8/119; 6.7%). The confirmation of isolated strains of BPIV3 was done by the virus-neutralization test. In addition, all samples of bovine nasal swabs were also examined for the presence of BPIV3 virus using RT-PCR with primers specific for the part of HN gene. The presence of BPIV3 was detected in eight samples (8/119; 6.7%) that were also positive upon virus isolation. The molecular characterization based on nucleotide sequencing of the part of the HN gene showed that all BPIV3 isolates belonged to genotype C of BPIV3. They branched in one distinct cluster with three different branches, but these branches were very similar to each other (98.1% to 99.8%). Serbian BPIV3c isolates were most similar to the Chinese BPIV3c isolates SD0805, SD0809 and SD0835 (from 97.92% to 99.7%), and to South Korean (12Q061), Japanese (HS9) and American (TVMDL16 and TVMDL20) BPIV3c strains (from 97.1% to 98.8%), and distinct from American (TVMDL15and TVMDL17) and Australian (Q5592) BPI3V genotype B strains (only 79.9% to 82.3% similarity), as well as from the genotype A BPIV3 strains from different countries published in GenBank., Ukupno je ispitano 119 uzoraka nosnih briseva goveda na prisustvo parainfluenca 3 virusa goveda (bovine parainfluenza virus type, eng. - 3 BPIV3). Iz svih uzoraka nosnih briseva je vršena izolacija virusa na ćelijskoj liniji MDBK. Pojava citopatogenog efekta na kulturi ćelija, nakon 48h, odnosno 72h, utvrđena je kod osam uzoraka nosnih briseva (8/119; 6.7%). Identifikacija izolovanih sojeva BPIV3 je izvršena primenom virus neutralizacionog testa. Dodatno, svi uzorci nosnih briseva goveda su ispitani na prisustvo BPIV3 i primenom metode RT-PCR uz korišćenje prajmera specifičnih za deo HN gena virusa. Prisustvo virusne nukleinske kiseline je utvrđeno kod osam uzoraka nosnih briseva (8/119; 6.7%), koji su bili pozitivni i na izolaciji virusa. Molekularna karakterizacija zasnovana na sekvenciranju dela HN gena izolata BPIV3 iz Srbije je potvrdila da svi pripadaju genotipu C BPIV3 (BPIV3c). Oni su se u filogenetskom stablu granali u tri različite grane koje su međusobno veoma slične (98.1% do 99.8%). Izolati BPIV3 iz Srbije su pokazali visok stepen sličnosti nukleotidnih sekvenci sa BPIV3c sojevima SD0805, SD0809 i SD0835 iz Kine (97.92% do 99.7%), odnosno sa BPIV3c sojevima 12Q061 iz Južne Koreje, HS9 iz Japana i TVMDL16 i TVMDL20 iz Amerike (sličnost od 97.1% do 98.8%), kao i različitosti u odnosu na nukleotidne sekvence sojeva TVMDL15 i TVMDL17 izolovanih u Americi i soja Q5592 izolovanog u Australiji, a koji su pripadali genotipu B BPIV3 (sličnost od 79.9% do 82.3%). Slična razlika je utvrđena i sa nukleotidnim sekvencama sojeva virusa, poreklom iz različitih država, svrstanih u genotip A BPIV3, a objavljenih u genskoj bazi podataka (NCBI GenBank).",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia, Izolacija i molekularna detekcija parainfluenca 3 virusa kod goveda u Srbiji",
volume = "66",
number = "4",
pages = "509-519",
doi = "10.1515/acve-2016-0044"
}

Veljović, L., Knežević, A., Milić, N., Krnjaić, D., Miković, R., Zorić, A., Marković, M., Milićević, V., Stamenković, M., Stanojević, M., Maksimović-Zorić, J., Petrović, T.,& Nišavić, J.. (2016). Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(4), 509-519.
https://doi.org/10.1515/acve-2016-0044

Veljović L, Knežević A, Milić N, Krnjaić D, Miković R, Zorić A, Marković M, Milićević V, Stamenković M, Stanojević M, Maksimović-Zorić J, Petrović T, Nišavić J. Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia. in Acta Veterinaria-Beograd. 2016;66(4):509-519.
doi:10.1515/acve-2016-0044 .

Veljović, Ljubiša, Knežević, Aleksandra, Milić, Nenad, Krnjaić, Dejan, Miković, Radoš, Zorić, Andrea, Marković, Maja, Milićević, Vesna, Stamenković, Miodrag, Stanojević, Maja, Maksimović-Zorić, Jelena, Petrović, Tamaš, Nišavić, Jakov, "Isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in Serbia" in Acta Veterinaria-Beograd, 66, no. 4 (2016):509-519,
https://doi.org/10.1515/acve-2016-0044 . .

TY  - JOUR
AU  - Zorić-Maksimović, Jelena
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Pavlović, Ivan
AU  - Radosavljević, Vladimir
AU  - Valčić, Miroslav
AU  - Glišić, Mileva
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1329
AB  - Bluetongue disease is non-contagious, vector borne, viral disease mainly of sheep but also of other domestic and wild ruminants. Bluetongue virus (BTV) belongs to the family Reoviridae, genus Orbivirus and is characterized by segmented double-stranded RNA. Virus is transmitted from one to another susceptible animal by hematophagous insects of the genus Culicoides. According to official data, between 2002 and 2014, Serbia has belonged to BTV free countries. After that, the first outbreak occurred in August 2014. The last case was reported in December of the same year. During 2015, 74 samples were examined for exclusion of bluetongue disease: 8 in cattle, 65 in sheep and one in goat. In order to detect viral genome, 73 blood samples and one tissue sample were examined by reverse transcription - polymerase chain reaction (RT-PCR). None of tested samples was confirmed to be BTV positive. Following the Instruction of the Ministry of Agriculture and Environmental Protection - Veterinary Directorate, monitoring program for Bluetongue disease in Serbia started from October 2015. The program consists of insect identification and detection of viral genome in Culicoides spp. by RT-PCR assay. Of the 80 samples that were received during the program realization in 2015, only four, which were collected during late autumn, have contained insects of Culicoides spp. In none of them, BTV was detected. For differential diagnosis, 65 ovine blood samples were examined for the presence of viruses of contagious ecthyma, sheep and goat pox as well as eight bovine blood samples were tested for viruses of bovine viral diarrhea, infectious bovine rhinotracheitis / pustular vulvovaginitis and malignant catarrhal fever. The samples were analyzed using molecular methods (PCR and RT-PCR). Only two bovine blood samples gave positive reaction for the presence of bovine viral diarrhea virus.
AB  - Bolest plavog jezika je nekontagiozno, vektorski uslovljeno virusno oboljenje koje se javlja uglavnom kod ovaca, ali i kod ostalih domaćih i divljih preživara. Virus plavog jezika (Bluetongue Virus - BTV) pripada familiji Reoviridae, rod Orbivirus a karakteriše ga segmentirana dvolančana RNK. Virus među prijemčivim životinjskim vrstama prenosi hematofagni insekt iz roda Culicoides. Prema zvaničnim podacima, Srbija se u periodu između 2002. i 2014.godine smatrala zemljom slobodnom of BTV. Nakon toga, bolest se pojavila prvi put u avgustu 2014. Poslednji slučaj prijavljen je u decembru iste godine. Tokom 2015. Godine ispitana su 74 uzorka sa ciljem isključivanja bolesti plavog jezika, i to 8 uzoraka poreklom od goveda, 65 od ovaca i jedan od koza. U cilju detekcije virusnog genoma 73 uzorka krvi i jedan uzorak tkiva su ispitani metodom reverzibilne lančane reakcije polimeraze (RT-PCR). Ni jedan od testiranih uzoraka nije bio pozitivan na BTV. U skladu sa Instrukcijom Ministarstvo poljoprivrede i zaštite životne sredine - Uprave za veterinu, u oktobru 2015. godine započet je program monitoringa bolesti plavog jezika u Srbiji. Program podrazumeva identifikaciju insekata i detektovanje genoma virusa kod Culicoides spp. primenom metode RT-PCR. Od ukupno 80 uzoraka koji su primljeni na ispitivanje tokom realizacije programa u 2015. godini, samo četiri uzorka koji su prikupljeni tokom kasne jeseni sadržali su insekte iz roda Culicoides spp. Ni u jednom od uzoraka nije detektovan BTV. U svrhu diferencijalne dijagnostike 65 uzoraka ovčije krvi ispitano je na prisustvo virusa kontagioznog ektima, boginja koza i ovaca, a osam uzoraka krvi goveda testirano je na viruse goveđe virusne dijareje, infektivnog goveđeg rihotraheitisa / pustularnog vulvovaginitisa i maligne kataralne groznice. Uzorci su analizirani primenom molekularnih metoda (PCR i RT-PCR). Kod samo dva uzorka krvi goveda ustanovljena je pozitivna reakcija na prisustvo virusa goveđe virusne dijareje.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Bluetongue disease: Epizootiology situation in Serbia in 2015, diagnosis and differential diagnosis
T1  - Bolest plavog jezika - epizootiološka situacija u Srbiji u 2015. godini, dijagnostika i diferencijalna dijagnostika
VL  - 9
IS  - 1
SP  - 13
EP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1329
ER  - 

@article{
author = "Zorić-Maksimović, Jelena and Milićević, Vesna and Veljović, Ljubiša and Pavlović, Ivan and Radosavljević, Vladimir and Valčić, Miroslav and Glišić, Mileva",
year = "2016",
abstract = "Bluetongue disease is non-contagious, vector borne, viral disease mainly of sheep but also of other domestic and wild ruminants. Bluetongue virus (BTV) belongs to the family Reoviridae, genus Orbivirus and is characterized by segmented double-stranded RNA. Virus is transmitted from one to another susceptible animal by hematophagous insects of the genus Culicoides. According to official data, between 2002 and 2014, Serbia has belonged to BTV free countries. After that, the first outbreak occurred in August 2014. The last case was reported in December of the same year. During 2015, 74 samples were examined for exclusion of bluetongue disease: 8 in cattle, 65 in sheep and one in goat. In order to detect viral genome, 73 blood samples and one tissue sample were examined by reverse transcription - polymerase chain reaction (RT-PCR). None of tested samples was confirmed to be BTV positive. Following the Instruction of the Ministry of Agriculture and Environmental Protection - Veterinary Directorate, monitoring program for Bluetongue disease in Serbia started from October 2015. The program consists of insect identification and detection of viral genome in Culicoides spp. by RT-PCR assay. Of the 80 samples that were received during the program realization in 2015, only four, which were collected during late autumn, have contained insects of Culicoides spp. In none of them, BTV was detected. For differential diagnosis, 65 ovine blood samples were examined for the presence of viruses of contagious ecthyma, sheep and goat pox as well as eight bovine blood samples were tested for viruses of bovine viral diarrhea, infectious bovine rhinotracheitis / pustular vulvovaginitis and malignant catarrhal fever. The samples were analyzed using molecular methods (PCR and RT-PCR). Only two bovine blood samples gave positive reaction for the presence of bovine viral diarrhea virus., Bolest plavog jezika je nekontagiozno, vektorski uslovljeno virusno oboljenje koje se javlja uglavnom kod ovaca, ali i kod ostalih domaćih i divljih preživara. Virus plavog jezika (Bluetongue Virus - BTV) pripada familiji Reoviridae, rod Orbivirus a karakteriše ga segmentirana dvolančana RNK. Virus među prijemčivim životinjskim vrstama prenosi hematofagni insekt iz roda Culicoides. Prema zvaničnim podacima, Srbija se u periodu između 2002. i 2014.godine smatrala zemljom slobodnom of BTV. Nakon toga, bolest se pojavila prvi put u avgustu 2014. Poslednji slučaj prijavljen je u decembru iste godine. Tokom 2015. Godine ispitana su 74 uzorka sa ciljem isključivanja bolesti plavog jezika, i to 8 uzoraka poreklom od goveda, 65 od ovaca i jedan od koza. U cilju detekcije virusnog genoma 73 uzorka krvi i jedan uzorak tkiva su ispitani metodom reverzibilne lančane reakcije polimeraze (RT-PCR). Ni jedan od testiranih uzoraka nije bio pozitivan na BTV. U skladu sa Instrukcijom Ministarstvo poljoprivrede i zaštite životne sredine - Uprave za veterinu, u oktobru 2015. godine započet je program monitoringa bolesti plavog jezika u Srbiji. Program podrazumeva identifikaciju insekata i detektovanje genoma virusa kod Culicoides spp. primenom metode RT-PCR. Od ukupno 80 uzoraka koji su primljeni na ispitivanje tokom realizacije programa u 2015. godini, samo četiri uzorka koji su prikupljeni tokom kasne jeseni sadržali su insekte iz roda Culicoides spp. Ni u jednom od uzoraka nije detektovan BTV. U svrhu diferencijalne dijagnostike 65 uzoraka ovčije krvi ispitano je na prisustvo virusa kontagioznog ektima, boginja koza i ovaca, a osam uzoraka krvi goveda testirano je na viruse goveđe virusne dijareje, infektivnog goveđeg rihotraheitisa / pustularnog vulvovaginitisa i maligne kataralne groznice. Uzorci su analizirani primenom molekularnih metoda (PCR i RT-PCR). Kod samo dva uzorka krvi goveda ustanovljena je pozitivna reakcija na prisustvo virusa goveđe virusne dijareje.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Bluetongue disease: Epizootiology situation in Serbia in 2015, diagnosis and differential diagnosis, Bolest plavog jezika - epizootiološka situacija u Srbiji u 2015. godini, dijagnostika i diferencijalna dijagnostika",
volume = "9",
number = "1",
pages = "13-22",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1329"
}

Zorić-Maksimović, J., Milićević, V., Veljović, L., Pavlović, I., Radosavljević, V., Valčić, M.,& Glišić, M.. (2016). Bluetongue disease: Epizootiology situation in Serbia in 2015, diagnosis and differential diagnosis. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 9(1), 13-22.
https://hdl.handle.net/21.15107/rcub_veterinar_1329

Zorić-Maksimović J, Milićević V, Veljović L, Pavlović I, Radosavljević V, Valčić M, Glišić M. Bluetongue disease: Epizootiology situation in Serbia in 2015, diagnosis and differential diagnosis. in Arhiv veterinarske medicine. 2016;9(1):13-22.
https://hdl.handle.net/21.15107/rcub_veterinar_1329 .

Zorić-Maksimović, Jelena, Milićević, Vesna, Veljović, Ljubiša, Pavlović, Ivan, Radosavljević, Vladimir, Valčić, Miroslav, Glišić, Mileva, "Bluetongue disease: Epizootiology situation in Serbia in 2015, diagnosis and differential diagnosis" in Arhiv veterinarske medicine, 9, no. 1 (2016):13-22,
https://hdl.handle.net/21.15107/rcub_veterinar_1329 .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
AU  - Ivović, V.
AU  - Maksimović-Zorić, Jelena
AU  - Radosavljević, Vladimir
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1065
AB  - Classical swine fever (CSF) is a highly contagious disease of pigs leading to significant economic losses worldwide. Classical swine fever virus can be classified into three genogroups, each consisting of three or four subgroups. However, there is a lack of knowledge on the genotypes of CSFV isolates in Republic of Serbia. This study, based on the sequences analysis of partial E2 gene and 5' non coding region (NCR) of 15 CSFV isolated during 2006-2008 from domestic pigs, revealed that all were clustered into genetic group 2.3. Additionally, we showed that the two most often used real time RT-PCR assays were able to detect all local CSF viruses circulated in Serbia in the last years during intensive vaccination campaign against CSF.
AB  - Klasična kuga svinja (CSF) je visoko kontagiozno oboljenje svinja koje dovodi do značajnih ekonomskih gubitaka širom sveta. Na osnovu genetske strukture, virus klasicne kuge svinja podeljen je utri genogrupe, od kojih svaka ima tri ili četiri podgrupe. Nedostaju podaci o tome koji genotipovi virusa klasične kuge svinja na teritoriji Republike Srbije cirkulišu u prijemčivoj populaciji. Sekvencioniranjem dela E2 gena i 5’nekodirajućeg regiona (NCR) 15 izolata virusa klasične kuge svinja prikupljenih u periodu od 2006-2008 godine, poreklom od domaćih svinja, dokazano je da svi pripadaju genetskoj grupi 2.3. Uz to, dokazano je i da je upotrebom dva najčešće korišćena real time RT-PCR protokola moguće detektovati sve lokalne izolate virusa klasične kuge svinja koji cirkulišu u Srbiji poslednjih godina, u kojoj se uporedo vršila i intenzivna vakcinacija protiv ove bolesti.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Detection and genotyping of classical swine fever virus isolates in Serbia
T1  - Detekcija i genotipizacija izolata virusa klasične kuge svinja u Srbiji
VL  - 63
IS  - 2-3
SP  - 191
EP  - 200
DO  - 10.2298/AVB1303191M
ER  - 

@article{
author = "Milićević, Vesna and Radojičić, Sonja and Valčić, Miroslav and Ivović, V. and Maksimović-Zorić, Jelena and Radosavljević, Vladimir",
year = "2013",
abstract = "Classical swine fever (CSF) is a highly contagious disease of pigs leading to significant economic losses worldwide. Classical swine fever virus can be classified into three genogroups, each consisting of three or four subgroups. However, there is a lack of knowledge on the genotypes of CSFV isolates in Republic of Serbia. This study, based on the sequences analysis of partial E2 gene and 5' non coding region (NCR) of 15 CSFV isolated during 2006-2008 from domestic pigs, revealed that all were clustered into genetic group 2.3. Additionally, we showed that the two most often used real time RT-PCR assays were able to detect all local CSF viruses circulated in Serbia in the last years during intensive vaccination campaign against CSF., Klasična kuga svinja (CSF) je visoko kontagiozno oboljenje svinja koje dovodi do značajnih ekonomskih gubitaka širom sveta. Na osnovu genetske strukture, virus klasicne kuge svinja podeljen je utri genogrupe, od kojih svaka ima tri ili četiri podgrupe. Nedostaju podaci o tome koji genotipovi virusa klasične kuge svinja na teritoriji Republike Srbije cirkulišu u prijemčivoj populaciji. Sekvencioniranjem dela E2 gena i 5’nekodirajućeg regiona (NCR) 15 izolata virusa klasične kuge svinja prikupljenih u periodu od 2006-2008 godine, poreklom od domaćih svinja, dokazano je da svi pripadaju genetskoj grupi 2.3. Uz to, dokazano je i da je upotrebom dva najčešće korišćena real time RT-PCR protokola moguće detektovati sve lokalne izolate virusa klasične kuge svinja koji cirkulišu u Srbiji poslednjih godina, u kojoj se uporedo vršila i intenzivna vakcinacija protiv ove bolesti.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Detection and genotyping of classical swine fever virus isolates in Serbia, Detekcija i genotipizacija izolata virusa klasične kuge svinja u Srbiji",
volume = "63",
number = "2-3",
pages = "191-200",
doi = "10.2298/AVB1303191M"
}

Milićević, V., Radojičić, S., Valčić, M., Ivović, V., Maksimović-Zorić, J.,& Radosavljević, V.. (2013). Detection and genotyping of classical swine fever virus isolates in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(2-3), 191-200.
https://doi.org/10.2298/AVB1303191M

Milićević V, Radojičić S, Valčić M, Ivović V, Maksimović-Zorić J, Radosavljević V. Detection and genotyping of classical swine fever virus isolates in Serbia. in Acta Veterinaria-Beograd. 2013;63(2-3):191-200.
doi:10.2298/AVB1303191M .

Milićević, Vesna, Radojičić, Sonja, Valčić, Miroslav, Ivović, V., Maksimović-Zorić, Jelena, Radosavljević, Vladimir, "Detection and genotyping of classical swine fever virus isolates in Serbia" in Acta Veterinaria-Beograd, 63, no. 2-3 (2013):191-200,
https://doi.org/10.2298/AVB1303191M . .

TY  - JOUR
AU  - Maksimović-Zorić, Jelena
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Petrović, Tamaš
AU  - Valčić, Miroslav
AU  - Plavšić, Branislav
AU  - Vranješ, N.
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/994
AB  - Rabies is a disease that occurs both in animals and people, with relatively long period of incubation, intense clinical course and fatal ending. Majority of cases of illnes and death in people are caused by the standard rabies virus. According to books of regulations on suppression and eradiction of rabies from 1988. and 2009., each suspected rabies has to be laboratory confirmed. From 2006. to 2012. within the passive surveilance, 3549 samples of brain tissue were examined for the presence of rabies virus. The virus was confirmed in 923 samples by using the method of direct imunofluorescence. From 2006. to 2012. there were diagnosed 192, 160, 233, 181, 104, 43 and 10 positive cases, respectively. For the purpose of rabies eradiction at the territory of the Republic of Serbia, a regional project of oral vaccination of foxes against rabies has been implemented since 2010.Within monitoring of the effectiveness of oral vaccination against rabies which was carried out at the end of 2011. and at the beginning of 2012., there were examined 1385 samples, out of which 11 reacted positively. The virus was dominantly present in the fox population. During 2008. and 2009., a relatively large number of rabid cats was registered, what indicates a transmission of rabies from the fox to the cat population. The incidence of rabies at the territory of Serbia significantly differs from one epizootic area to another. Northern parts of the country (Sombor and Subotica epizootic regions) have more favorable situation with only two cases of rabies for the last 7 years. In the period until 2010., in Pozarevac, Novi Sad, Jagodina, Pancevo, Belgrade and Nis epizootic region, a gradual decline in number of positive cases was noticed. International project for oral vaccination of foxes against rabies which has been implemented at the territory of the Republic of Serbia and neighboring countries, influenced a significant reduction of registered rabies cases.
AB  - Besnilo je bolest životinja i ljudi koja se odlikuje relativno dugim inkubacionim periodom i dramatičnim kliničkim tokom, koji se uvek završava smrtnim ishodom. Najveći broj slučajeva oboljenja i smrti ljudi potiče od infekcije klasičnim virusom besnila. Prema pravilnicima koji se bave suzbijanjem i iskorenjivanjem besnila iz 1988. i 2009. godine obavezna je laboratorijska potvrda svake klinički opravdane sumnje na besnilo. Od 2006. do 2012. godine u okviru pasivnog nadzora pregledano je 3549 uzoraka moždanog tkiva na prisustvo virusa besnila. Metodom direktne imunofluorescencije virus je dokazan u 923 uzoraka. Po godinama, od 2006. do 2012. godine dijagnostikovano je 192, 160, 233, 181, 104, 43 i 10 pozitivnih slučajeva. U cilju iskorenjivanja besnila na teritoriji Republike Srbije od 2010. godine se sprovodi regionalni projekat oralne vakcinacije lisica protiv besnila. U okviru monitoringa efikasnosti oralne vakcinacije lisica protiv besnila koji je sprovođen krajem 2011. i početkom 2012. godine ispitano je 1385 uzoraka od čega je 11 reagovalo pozitivno. Virus je dominantno prisutan u populaciji lisica. Tokom 2008. i 2009. godine dijagnostikavan je relativno veliki broj besnih mačaka, što ukazuje na prenos virusa iz populacije lisica u populaciju mačaka. Incidencija besnila na teritoriji Srbije se značajno razlikuje od jednog do drugog epizootiološkog područja. Severni delovi zemlje (somborsko i subotičko epizotiološko područje) imaju povoljniju epizootiološku situaciju (samo dva slučaja besnila u proteklih 7 godina). U periodu do 2010. godine na požarevačkom, novosadskom, jagodinskom, pančevačkom, beogradskom i niškom epizootiološkom području se uočava postepeni pad broja pozitivnih slučajeva. Međunarodni projekat oralne vakcinacije lisica protiv besnila koji se sprovodi na teritoriji Republike Srbije i okolnih zemalja uticao je na to da se broj prijavljenih slučajeva besnila u Srbiji drastično smanji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Rabies: Epizootiological situation at the territory of Serbia and countries in the region from 2006. to 2012.
T1  - Besnilo - epizootiološka situacija na teritoriji Srbije i zemalja u okruženju od 2006. do 2012. godine
VL  - 67
IS  - 5-6
SP  - 377
EP  - 394
DO  - 10.2298/VETGL1306377M
ER  - 

@article{
author = "Maksimović-Zorić, Jelena and Milićević, Vesna and Veljović, Ljubiša and Petrović, Tamaš and Valčić, Miroslav and Plavšić, Branislav and Vranješ, N.",
year = "2013",
abstract = "Rabies is a disease that occurs both in animals and people, with relatively long period of incubation, intense clinical course and fatal ending. Majority of cases of illnes and death in people are caused by the standard rabies virus. According to books of regulations on suppression and eradiction of rabies from 1988. and 2009., each suspected rabies has to be laboratory confirmed. From 2006. to 2012. within the passive surveilance, 3549 samples of brain tissue were examined for the presence of rabies virus. The virus was confirmed in 923 samples by using the method of direct imunofluorescence. From 2006. to 2012. there were diagnosed 192, 160, 233, 181, 104, 43 and 10 positive cases, respectively. For the purpose of rabies eradiction at the territory of the Republic of Serbia, a regional project of oral vaccination of foxes against rabies has been implemented since 2010.Within monitoring of the effectiveness of oral vaccination against rabies which was carried out at the end of 2011. and at the beginning of 2012., there were examined 1385 samples, out of which 11 reacted positively. The virus was dominantly present in the fox population. During 2008. and 2009., a relatively large number of rabid cats was registered, what indicates a transmission of rabies from the fox to the cat population. The incidence of rabies at the territory of Serbia significantly differs from one epizootic area to another. Northern parts of the country (Sombor and Subotica epizootic regions) have more favorable situation with only two cases of rabies for the last 7 years. In the period until 2010., in Pozarevac, Novi Sad, Jagodina, Pancevo, Belgrade and Nis epizootic region, a gradual decline in number of positive cases was noticed. International project for oral vaccination of foxes against rabies which has been implemented at the territory of the Republic of Serbia and neighboring countries, influenced a significant reduction of registered rabies cases., Besnilo je bolest životinja i ljudi koja se odlikuje relativno dugim inkubacionim periodom i dramatičnim kliničkim tokom, koji se uvek završava smrtnim ishodom. Najveći broj slučajeva oboljenja i smrti ljudi potiče od infekcije klasičnim virusom besnila. Prema pravilnicima koji se bave suzbijanjem i iskorenjivanjem besnila iz 1988. i 2009. godine obavezna je laboratorijska potvrda svake klinički opravdane sumnje na besnilo. Od 2006. do 2012. godine u okviru pasivnog nadzora pregledano je 3549 uzoraka moždanog tkiva na prisustvo virusa besnila. Metodom direktne imunofluorescencije virus je dokazan u 923 uzoraka. Po godinama, od 2006. do 2012. godine dijagnostikovano je 192, 160, 233, 181, 104, 43 i 10 pozitivnih slučajeva. U cilju iskorenjivanja besnila na teritoriji Republike Srbije od 2010. godine se sprovodi regionalni projekat oralne vakcinacije lisica protiv besnila. U okviru monitoringa efikasnosti oralne vakcinacije lisica protiv besnila koji je sprovođen krajem 2011. i početkom 2012. godine ispitano je 1385 uzoraka od čega je 11 reagovalo pozitivno. Virus je dominantno prisutan u populaciji lisica. Tokom 2008. i 2009. godine dijagnostikavan je relativno veliki broj besnih mačaka, što ukazuje na prenos virusa iz populacije lisica u populaciju mačaka. Incidencija besnila na teritoriji Srbije se značajno razlikuje od jednog do drugog epizootiološkog područja. Severni delovi zemlje (somborsko i subotičko epizotiološko područje) imaju povoljniju epizootiološku situaciju (samo dva slučaja besnila u proteklih 7 godina). U periodu do 2010. godine na požarevačkom, novosadskom, jagodinskom, pančevačkom, beogradskom i niškom epizootiološkom području se uočava postepeni pad broja pozitivnih slučajeva. Međunarodni projekat oralne vakcinacije lisica protiv besnila koji se sprovodi na teritoriji Republike Srbije i okolnih zemalja uticao je na to da se broj prijavljenih slučajeva besnila u Srbiji drastično smanji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Rabies: Epizootiological situation at the territory of Serbia and countries in the region from 2006. to 2012., Besnilo - epizootiološka situacija na teritoriji Srbije i zemalja u okruženju od 2006. do 2012. godine",
volume = "67",
number = "5-6",
pages = "377-394",
doi = "10.2298/VETGL1306377M"
}

Maksimović-Zorić, J., Milićević, V., Veljović, L., Petrović, T., Valčić, M., Plavšić, B.,& Vranješ, N.. (2013). Rabies: Epizootiological situation at the territory of Serbia and countries in the region from 2006. to 2012.. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(5-6), 377-394.
https://doi.org/10.2298/VETGL1306377M

Maksimović-Zorić J, Milićević V, Veljović L, Petrović T, Valčić M, Plavšić B, Vranješ N. Rabies: Epizootiological situation at the territory of Serbia and countries in the region from 2006. to 2012.. in Veterinarski Glasnik. 2013;67(5-6):377-394.
doi:10.2298/VETGL1306377M .

Maksimović-Zorić, Jelena, Milićević, Vesna, Veljović, Ljubiša, Petrović, Tamaš, Valčić, Miroslav, Plavšić, Branislav, Vranješ, N., "Rabies: Epizootiological situation at the territory of Serbia and countries in the region from 2006. to 2012." in Veterinarski Glasnik, 67, no. 5-6 (2013):377-394,
https://doi.org/10.2298/VETGL1306377M . .