TY  - JOUR
AU  - Lužajić Božinovski, Tijana
AU  - Danilović Luković, Jelena
AU  - Nikolić, Anja
AU  - Radovanović, Anita
AU  - Marković, Danica
AU  - Kovačević Filipović, Milica
AU  - Vasić, Mirjana
AU  - Milošević, Ivan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3047
AB  - Epidermis stem cells have a crucial role through the processes of proliferation and
differentiation, to replace cells that are constantly lost during tissue turnover or
following injury. On the other hand, thyroid hormones regulate the proliferation and
differentiation of epidermal cells and thus significantly influence the homeostasis
of the skin. It is well known that maternal hypothyroidism during pregnancy leads
to impaired development of many organ systems in their offspring. However, there
is a lack of data about the influence of maternal subclinical hypothyroidism during
pregnancy and lactation on the development of the skin and its derivatives in the litter.
The aim of this study was to investigate the effects of maternal thyroid dysfunction on
the development of the skin and its derivatives in their offspring in the early postnatal
period. Antithyroid substance 6-n-propyl-2-thiouracil was added into the drinking water
to female Albino Oxfords rats from the beginning of pregnancy and during lactation,
with the aim to induce subclinical and overt form of hypothyroidism. Skin samples
were taken from male pups within twenty-four hours and seven days after birth. The
main findings of this investigation were that both forms of maternal hypothyroidism
lead to serious damage of the epidermis in pups in terms of pronounced hyperkeratosis
and reduction of the germinal layer along with a reduced number of hair follicles and
their delayed morphogenesis. Epidermal impairments were more pronounced in pups
with the overt form of hypothyroidism while offspring with the subclinical form had
impairments that were less pronounced and delayed in occurrence.
PB  - Beograd : Fakultet veterinarske medicine
T2  - Acta Veterinaria
T1  - Maternal subclinical and clinical hypothyroidism effects on rat offspring: a story of the skin and its derivatives
VL  - 73
IS  - 2
SP  - 231
EP  - 248
DO  - 10.2478/acve-2023-0018
ER  - 

@article{
author = "Lužajić Božinovski, Tijana and Danilović Luković, Jelena and Nikolić, Anja and Radovanović, Anita and Marković, Danica and Kovačević Filipović, Milica and Vasić, Mirjana and Milošević, Ivan",
year = "2023",
abstract = "Epidermis stem cells have a crucial role through the processes of proliferation and
differentiation, to replace cells that are constantly lost during tissue turnover or
following injury. On the other hand, thyroid hormones regulate the proliferation and
differentiation of epidermal cells and thus significantly influence the homeostasis
of the skin. It is well known that maternal hypothyroidism during pregnancy leads
to impaired development of many organ systems in their offspring. However, there
is a lack of data about the influence of maternal subclinical hypothyroidism during
pregnancy and lactation on the development of the skin and its derivatives in the litter.
The aim of this study was to investigate the effects of maternal thyroid dysfunction on
the development of the skin and its derivatives in their offspring in the early postnatal
period. Antithyroid substance 6-n-propyl-2-thiouracil was added into the drinking water
to female Albino Oxfords rats from the beginning of pregnancy and during lactation,
with the aim to induce subclinical and overt form of hypothyroidism. Skin samples
were taken from male pups within twenty-four hours and seven days after birth. The
main findings of this investigation were that both forms of maternal hypothyroidism
lead to serious damage of the epidermis in pups in terms of pronounced hyperkeratosis
and reduction of the germinal layer along with a reduced number of hair follicles and
their delayed morphogenesis. Epidermal impairments were more pronounced in pups
with the overt form of hypothyroidism while offspring with the subclinical form had
impairments that were less pronounced and delayed in occurrence.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Acta Veterinaria",
title = "Maternal subclinical and clinical hypothyroidism effects on rat offspring: a story of the skin and its derivatives",
volume = "73",
number = "2",
pages = "231-248",
doi = "10.2478/acve-2023-0018"
}

Lužajić Božinovski, T., Danilović Luković, J., Nikolić, A., Radovanović, A., Marković, D., Kovačević Filipović, M., Vasić, M.,& Milošević, I.. (2023). Maternal subclinical and clinical hypothyroidism effects on rat offspring: a story of the skin and its derivatives. in Acta Veterinaria
Beograd : Fakultet veterinarske medicine., 73(2), 231-248.
https://doi.org/10.2478/acve-2023-0018

Lužajić Božinovski T, Danilović Luković J, Nikolić A, Radovanović A, Marković D, Kovačević Filipović M, Vasić M, Milošević I. Maternal subclinical and clinical hypothyroidism effects on rat offspring: a story of the skin and its derivatives. in Acta Veterinaria. 2023;73(2):231-248.
doi:10.2478/acve-2023-0018 .

Lužajić Božinovski, Tijana, Danilović Luković, Jelena, Nikolić, Anja, Radovanović, Anita, Marković, Danica, Kovačević Filipović, Milica, Vasić, Mirjana, Milošević, Ivan, "Maternal subclinical and clinical hypothyroidism effects on rat offspring: a story of the skin and its derivatives" in Acta Veterinaria, 73, no. 2 (2023):231-248,
https://doi.org/10.2478/acve-2023-0018 . .

TY  - CONF
AU  - Milošević, Ivan
AU  - Lužajić Božinovski, Tijana
AU  - Danilović Luković, Jelena
AU  - Gajdov, Vladimir
AU  - Marković, Danica
AU  - Kovačević Filipović, Milica
AU  - Radovanović, Anita
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2609
AB  - Overt and subclinical maternal hypothyroidism affects cartilage and bone extracellular matrix formation during fetal endochondral bone development. Less is known about the
effect maternal hypothyroidism has on cell proliferation and death of growth plate chondrocytes.
This study aimed to evaluate the expression of proliferation (Ki67), autophagy (LC3) and apoptosis (caspase 3) markers in the epiphyseal cartilage of the tibia in pups from 
subclinical and overt hypothyroid does, in their early postnatal development.Hypothyroidism was induced with propylthiouracil using low (1.5 mg/L) for subclinical (H1) and high dose (150 mg/L) for overt (H2) form. Propylthiouracil was
administered through drinking water in pregnant Albino Oxford does from the first day of gravidity and during lactation. Control (C) group was not treated. Six, seven-day-old male
pups from each group were euthanized. Histological examinations were performed on paraffin sections of the proximal tibial growth plate. Immunohistochemistry was used to assess
the expression of Ki67, LC3 and caspase 3. The number of Ki67 positive chondrocytes was evaluated in the resting and proliferating zone using Image J. The expression of LC3 was
performed by counting the number of positive dots in chondrocytes.The number of Ki67 positive chondrocytes was higher in both hypothyroid groups, while the number of LC3 positive dots per cell was lower indicating that the chondrocyte
cell cycle in hypothyroid animals is faster and that the basal level of autophagy is reduced due to frequent mitosis. Compared to controls, caspase 3 expression in the terminal
hypertrophic chondrocytes was reduced in H1 and completely absent in H2 pups which might indicate impaired differentiation.We conclude that both forms of maternal hypothyroidism
in rats lead to accelerated proliferation, slowed autophagy and
compromised apoptosis of terminal hypertrophic chondrocytes in the
early infantile period and a delay in cartilage to bone transition.
C3  - 9th International Conference Veterinary Science and Profession 2021, Zagreb
T1  - Hypothyroidism in pregnant rats affects proliferation and cell death of growth plate chondrocytes in pups
SP  - 114
EP  - 114
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2609
ER  - 

@conference{
author = "Milošević, Ivan and Lužajić Božinovski, Tijana and Danilović Luković, Jelena and Gajdov, Vladimir and Marković, Danica and Kovačević Filipović, Milica and Radovanović, Anita",
year = "2021",
abstract = "Overt and subclinical maternal hypothyroidism affects cartilage and bone extracellular matrix formation during fetal endochondral bone development. Less is known about the
effect maternal hypothyroidism has on cell proliferation and death of growth plate chondrocytes.
This study aimed to evaluate the expression of proliferation (Ki67), autophagy (LC3) and apoptosis (caspase 3) markers in the epiphyseal cartilage of the tibia in pups from 
subclinical and overt hypothyroid does, in their early postnatal development.Hypothyroidism was induced with propylthiouracil using low (1.5 mg/L) for subclinical (H1) and high dose (150 mg/L) for overt (H2) form. Propylthiouracil was
administered through drinking water in pregnant Albino Oxford does from the first day of gravidity and during lactation. Control (C) group was not treated. Six, seven-day-old male
pups from each group were euthanized. Histological examinations were performed on paraffin sections of the proximal tibial growth plate. Immunohistochemistry was used to assess
the expression of Ki67, LC3 and caspase 3. The number of Ki67 positive chondrocytes was evaluated in the resting and proliferating zone using Image J. The expression of LC3 was
performed by counting the number of positive dots in chondrocytes.The number of Ki67 positive chondrocytes was higher in both hypothyroid groups, while the number of LC3 positive dots per cell was lower indicating that the chondrocyte
cell cycle in hypothyroid animals is faster and that the basal level of autophagy is reduced due to frequent mitosis. Compared to controls, caspase 3 expression in the terminal
hypertrophic chondrocytes was reduced in H1 and completely absent in H2 pups which might indicate impaired differentiation.We conclude that both forms of maternal hypothyroidism
in rats lead to accelerated proliferation, slowed autophagy and
compromised apoptosis of terminal hypertrophic chondrocytes in the
early infantile period and a delay in cartilage to bone transition.",
journal = "9th International Conference Veterinary Science and Profession 2021, Zagreb",
title = "Hypothyroidism in pregnant rats affects proliferation and cell death of growth plate chondrocytes in pups",
pages = "114-114",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2609"
}

Milošević, I., Lužajić Božinovski, T., Danilović Luković, J., Gajdov, V., Marković, D., Kovačević Filipović, M.,& Radovanović, A.. (2021). Hypothyroidism in pregnant rats affects proliferation and cell death of growth plate chondrocytes in pups. in 9th International Conference Veterinary Science and Profession 2021, Zagreb, 114-114.
https://hdl.handle.net/21.15107/rcub_veterinar_2609

Milošević I, Lužajić Božinovski T, Danilović Luković J, Gajdov V, Marković D, Kovačević Filipović M, Radovanović A. Hypothyroidism in pregnant rats affects proliferation and cell death of growth plate chondrocytes in pups. in 9th International Conference Veterinary Science and Profession 2021, Zagreb. 2021;:114-114.
https://hdl.handle.net/21.15107/rcub_veterinar_2609 .

Milošević, Ivan, Lužajić Božinovski, Tijana, Danilović Luković, Jelena, Gajdov, Vladimir, Marković, Danica, Kovačević Filipović, Milica, Radovanović, Anita, "Hypothyroidism in pregnant rats affects proliferation and cell death of growth plate chondrocytes in pups" in 9th International Conference Veterinary Science and Profession 2021, Zagreb (2021):114-114,
https://hdl.handle.net/21.15107/rcub_veterinar_2609 .

TY  - CONF
AU  - Danilović Luković, Jelena
AU  - Radovanović, Anita
AU  - Milošević, Ivan
AU  - Lužajić Božinovski, Tijana
AU  - Milanović, Svetlana
AU  - Kovačević Filipović, Milica
AU  - Korać, Aleksandra
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2597
AB  - The stimulative effects of thyroid hormones on mitochondria are realized through 
both non-genomic and genomic mechanisms, affecting respiration, mitochondrial plasticity 
and biogenesis. The subclinical form of maternal hypothyroidism in rats induces significant 
reduction of mitochondria number but also an augmentation of their area in neonatal and 
early infantile offspring dyctiotene oocytes. This study aimed to investigate if this form of 
subclinical hypothyroidism affects mitochondrial morphology and distribution in the early 
prophase of meiosis I oocytes. It was performed on newborn control (C) (n=10) and 
hypothyroid (SCH) (n=10) female rat pups derived from control (n=6) and propylthiouracil
treated pregnant dams (n=6), respectively. Ovaries of all pups were removed and processed 
for transmission electron microscopy. The morphological features of mitochondria in the 
early prophase I oocytes until dyctiotene were assessed. No substantial differences were 
found in leptotene and zygotene oocytes in SCH group comparing to control, except just a 
few mitochondria characterized with shortened cristae, presence of wide pale area centrally 
positioned and membrane disruption. Pachytene mitochondria in treated pup oocytes were 
in great extent with disrupted membrane, shortened cristae and wide pale area centrally 
positioned while these features were rarely observed in control ones. Our results confirm 
altered mitochondria morphology found in primordial and primary follicles in case of 
maternal hypothyroidism, indicating their impaired function and possibly, propensity to 
programmed cell death. Further investigations may indicate to what extent pachytene, as a 
meiotic checkpoint, appears to be a milestone possibly predetermining the future of the 
cell.
PB  - Belgrade : Serbian Society for Mitochondrial and Free Radical Physiology
PB  - Belgrade : Ministry of Education, Science and Technological Development
PB  - Belgrade : University of Belgrade
PB  - Belgrade : Faculty of Biology of University of Belgrade
C3  - Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade
T1  - Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection
SP  - 103
EP  - 103
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2597
ER  - 

@conference{
author = "Danilović Luković, Jelena and Radovanović, Anita and Milošević, Ivan and Lužajić Božinovski, Tijana and Milanović, Svetlana and Kovačević Filipović, Milica and Korać, Aleksandra",
year = "2018",
abstract = "The stimulative effects of thyroid hormones on mitochondria are realized through 
both non-genomic and genomic mechanisms, affecting respiration, mitochondrial plasticity 
and biogenesis. The subclinical form of maternal hypothyroidism in rats induces significant 
reduction of mitochondria number but also an augmentation of their area in neonatal and 
early infantile offspring dyctiotene oocytes. This study aimed to investigate if this form of 
subclinical hypothyroidism affects mitochondrial morphology and distribution in the early 
prophase of meiosis I oocytes. It was performed on newborn control (C) (n=10) and 
hypothyroid (SCH) (n=10) female rat pups derived from control (n=6) and propylthiouracil
treated pregnant dams (n=6), respectively. Ovaries of all pups were removed and processed 
for transmission electron microscopy. The morphological features of mitochondria in the 
early prophase I oocytes until dyctiotene were assessed. No substantial differences were 
found in leptotene and zygotene oocytes in SCH group comparing to control, except just a 
few mitochondria characterized with shortened cristae, presence of wide pale area centrally 
positioned and membrane disruption. Pachytene mitochondria in treated pup oocytes were 
in great extent with disrupted membrane, shortened cristae and wide pale area centrally 
positioned while these features were rarely observed in control ones. Our results confirm 
altered mitochondria morphology found in primordial and primary follicles in case of 
maternal hypothyroidism, indicating their impaired function and possibly, propensity to 
programmed cell death. Further investigations may indicate to what extent pachytene, as a 
meiotic checkpoint, appears to be a milestone possibly predetermining the future of the 
cell.",
publisher = "Belgrade : Serbian Society for Mitochondrial and Free Radical Physiology, Belgrade : Ministry of Education, Science and Technological Development, Belgrade : University of Belgrade, Belgrade : Faculty of Biology of University of Belgrade",
journal = "Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade",
title = "Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection",
pages = "103-103",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2597"
}

Danilović Luković, J., Radovanović, A., Milošević, I., Lužajić Božinovski, T., Milanović, S., Kovačević Filipović, M.,& Korać, A.. (2018). Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection. in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade
Belgrade : Serbian Society for Mitochondrial and Free Radical Physiology., 103-103.
https://hdl.handle.net/21.15107/rcub_veterinar_2597

Danilović Luković J, Radovanović A, Milošević I, Lužajić Božinovski T, Milanović S, Kovačević Filipović M, Korać A. Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection. in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade. 2018;:103-103.
https://hdl.handle.net/21.15107/rcub_veterinar_2597 .

Danilović Luković, Jelena, Radovanović, Anita, Milošević, Ivan, Lužajić Božinovski, Tijana, Milanović, Svetlana, Kovačević Filipović, Milica, Korać, Aleksandra, "Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection" in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade (2018):103-103,
https://hdl.handle.net/21.15107/rcub_veterinar_2597 .

TY  - JOUR
AU  - Milošević, Ivan
AU  - Radovanović, Anita
AU  - Danilović-Luković, Jelena
AU  - Lužajić Božinovski, Tijana
AU  - Sourice-Petit, Sophie
AU  - Beck-Cormier, Sarah
AU  - Guicheux, Jerome
AU  - Vejnović, Branislav
AU  - Kovačević-Filipović, Milica
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1588
AB  - Maternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, influences the process of offspring endochondral ossification affecting proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups. Seven-day-old male pups (n=18) derived from control dams and dams treated with a low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water during pregnancy and lactation were used. Histomorphometric analysis of pups tibia proximal growth plate, expression of mRNA, immunohistochemical and histochemical visualization of extracellular matrix components was performed. The length of the tibia was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical and overt form were lower while the amount of glycosaminoglycans was higher when compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA indicated altered osteoclasts function while lower expression of dentin matrix acid protein-1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised bone formation in the overt form of hypothyroidism. The subclinical form of maternal hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in growth plate formation.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation
VL  - 68
IS  - 3
SP  - 301
EP  - 320
DO  - 10.2478/acve-2018-0026
ER  - 

@article{
author = "Milošević, Ivan and Radovanović, Anita and Danilović-Luković, Jelena and Lužajić Božinovski, Tijana and Sourice-Petit, Sophie and Beck-Cormier, Sarah and Guicheux, Jerome and Vejnović, Branislav and Kovačević-Filipović, Milica",
year = "2018",
abstract = "Maternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, influences the process of offspring endochondral ossification affecting proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups. Seven-day-old male pups (n=18) derived from control dams and dams treated with a low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water during pregnancy and lactation were used. Histomorphometric analysis of pups tibia proximal growth plate, expression of mRNA, immunohistochemical and histochemical visualization of extracellular matrix components was performed. The length of the tibia was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical and overt form were lower while the amount of glycosaminoglycans was higher when compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA indicated altered osteoclasts function while lower expression of dentin matrix acid protein-1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised bone formation in the overt form of hypothyroidism. The subclinical form of maternal hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in growth plate formation.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation",
volume = "68",
number = "3",
pages = "301-320",
doi = "10.2478/acve-2018-0026"
}

Milošević, I., Radovanović, A., Danilović-Luković, J., Lužajić Božinovski, T., Sourice-Petit, S., Beck-Cormier, S., Guicheux, J., Vejnović, B.,& Kovačević-Filipović, M.. (2018). Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(3), 301-320.
https://doi.org/10.2478/acve-2018-0026

Milošević I, Radovanović A, Danilović-Luković J, Lužajić Božinovski T, Sourice-Petit S, Beck-Cormier S, Guicheux J, Vejnović B, Kovačević-Filipović M. Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation. in Acta Veterinaria-Beograd. 2018;68(3):301-320.
doi:10.2478/acve-2018-0026 .

Milošević, Ivan, Radovanović, Anita, Danilović-Luković, Jelena, Lužajić Božinovski, Tijana, Sourice-Petit, Sophie, Beck-Cormier, Sarah, Guicheux, Jerome, Vejnović, Branislav, Kovačević-Filipović, Milica, "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation" in Acta Veterinaria-Beograd, 68, no. 3 (2018):301-320,
https://doi.org/10.2478/acve-2018-0026 . .

TY  - JOUR
AU  - Danilović-Luković, Jelena
AU  - Korac, Aleksandra
AU  - Milošević, Ivan
AU  - Lužajić, Tijana
AU  - Milanović, Zorana
AU  - Kovačević-Filipović, Milica
AU  - Radovanović, Anita
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1477
AB  - Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/ oocytes was significantly lower in the hypothyroid group. No significant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly reflecting that these proteins were not involved in the oogonia/ oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/ oocytes altered with the advanced process of autophagy.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats
VL  - 67
IS  - 1
SP  - 92
EP  - 106
DO  - 10.1515/acve-2017-0009
ER  - 

@article{
author = "Danilović-Luković, Jelena and Korac, Aleksandra and Milošević, Ivan and Lužajić, Tijana and Milanović, Zorana and Kovačević-Filipović, Milica and Radovanović, Anita",
year = "2017",
abstract = "Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/ oocytes was significantly lower in the hypothyroid group. No significant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly reflecting that these proteins were not involved in the oogonia/ oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/ oocytes altered with the advanced process of autophagy.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats",
volume = "67",
number = "1",
pages = "92-106",
doi = "10.1515/acve-2017-0009"
}

Danilović-Luković, J., Korac, A., Milošević, I., Lužajić, T., Milanović, Z., Kovačević-Filipović, M.,& Radovanović, A.. (2017). Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(1), 92-106.
https://doi.org/10.1515/acve-2017-0009

Danilović-Luković J, Korac A, Milošević I, Lužajić T, Milanović Z, Kovačević-Filipović M, Radovanović A. Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats. in Acta Veterinaria-Beograd. 2017;67(1):92-106.
doi:10.1515/acve-2017-0009 .

Danilović-Luković, Jelena, Korac, Aleksandra, Milošević, Ivan, Lužajić, Tijana, Milanović, Zorana, Kovačević-Filipović, Milica, Radovanović, Anita, "Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats" in Acta Veterinaria-Beograd, 67, no. 1 (2017):92-106,
https://doi.org/10.1515/acve-2017-0009 . .

TY  - CONF
AU  - Lužajić, Tijana
AU  - Milošević, Ivan
AU  - Danilović Luković, Jelena
AU  - Marković, Danica
AU  - Milošević, Sandra
AU  - Kovačević Filipović, Milica
AU  - Radovanović, Anita
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2605
AB  - Introduction. Thyroid hormones (TH) play a pivotal role in the development of mammalian skin and are necessary for both, the initiation and maintenance of hair growth. Hypothyroid rats showed  impaired epidermal proliferation, hair growth, and wound healing. Stem cells located at the bulges of the hair follicles are responsible for hair cycling and contribute to the regeneration of the new epidermis after wounding. Therefore a reduction in the num¬ber or function of the bulge stem cells could be responsible for this phenotype. 
We hypothesized that decrease in TH during prenatal development would affect bulge stem cell population.
Material and methods. Subclinical hypothyroidism was induced with propylthiouracil through drinking water in dose 1.5 mg/l in pregnant Albino Oxford rats from the first day of gravidity. Control group was untreated. Seven-day-old pups were euthanized and skin samples were taken from dorsal part of their bodies. For the immunohistochemical study PCNA (Santa Cruz Biotechnology) and NANOG (Thermo scientific) antibodies were used.
Results. . The number of hair follicles was reduced in hypothyroid pups compared to 
controls. They also had a decreased expression of PCNA in cells of inner and outer sheat of hair follicles, but increased number of NANOG positive bulge stem cells. 
Conclusion. This suggests that re¬duced activation of stem cells leading to their accumulation in the bulges which is associated with reduced stem cell prolif¬eration and mobilization.
PB  - Faculty of Veterinary Medicine, University of Zagreb
C3  - 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb
T1  - Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population
SP  - 152
EP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2605
ER  - 

@conference{
author = "Lužajić, Tijana and Milošević, Ivan and Danilović Luković, Jelena and Marković, Danica and Milošević, Sandra and Kovačević Filipović, Milica and Radovanović, Anita",
year = "2017",
abstract = "Introduction. Thyroid hormones (TH) play a pivotal role in the development of mammalian skin and are necessary for both, the initiation and maintenance of hair growth. Hypothyroid rats showed  impaired epidermal proliferation, hair growth, and wound healing. Stem cells located at the bulges of the hair follicles are responsible for hair cycling and contribute to the regeneration of the new epidermis after wounding. Therefore a reduction in the num¬ber or function of the bulge stem cells could be responsible for this phenotype. 
We hypothesized that decrease in TH during prenatal development would affect bulge stem cell population.
Material and methods. Subclinical hypothyroidism was induced with propylthiouracil through drinking water in dose 1.5 mg/l in pregnant Albino Oxford rats from the first day of gravidity. Control group was untreated. Seven-day-old pups were euthanized and skin samples were taken from dorsal part of their bodies. For the immunohistochemical study PCNA (Santa Cruz Biotechnology) and NANOG (Thermo scientific) antibodies were used.
Results. . The number of hair follicles was reduced in hypothyroid pups compared to 
controls. They also had a decreased expression of PCNA in cells of inner and outer sheat of hair follicles, but increased number of NANOG positive bulge stem cells. 
Conclusion. This suggests that re¬duced activation of stem cells leading to their accumulation in the bulges which is associated with reduced stem cell prolif¬eration and mobilization.",
publisher = "Faculty of Veterinary Medicine, University of Zagreb",
journal = "7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb",
title = "Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population",
pages = "152-152",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2605"
}

Lužajić, T., Milošević, I., Danilović Luković, J., Marković, D., Milošević, S., Kovačević Filipović, M.,& Radovanović, A.. (2017). Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population. in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb
Faculty of Veterinary Medicine, University of Zagreb., 152-152.
https://hdl.handle.net/21.15107/rcub_veterinar_2605

Lužajić T, Milošević I, Danilović Luković J, Marković D, Milošević S, Kovačević Filipović M, Radovanović A. Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population. in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb. 2017;:152-152.
https://hdl.handle.net/21.15107/rcub_veterinar_2605 .

Lužajić, Tijana, Milošević, Ivan, Danilović Luković, Jelena, Marković, Danica, Milošević, Sandra, Kovačević Filipović, Milica, Radovanović, Anita, "Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population" in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb (2017):152-152,
https://hdl.handle.net/21.15107/rcub_veterinar_2605 .

TY  - CONF
AU  - Milošević, Ivan
AU  - Danilović Luković, Jelena
AU  - Lužajić, Tijana
AU  - Marković, Danica
AU  - Radovanović, Anita
AU  - Sourice-Petit, Sophie
AU  - Guicheux, Jerome
AU  - Kovačević Filipović, Milica
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2603
AB  - Thyroid hormones (TH) are indispensable for normal development in mammals. The effects of maternal hypothyroidism on development and function of different organs of their offspring are well known. It is unclear how its subclinical form affects some of these processes, including endochondral ossification during the prenatal and early postnatal development.
The aim of the study was to assess bone formation by defining the expression of tartarate-resistant acid phosphatase (TRAP), alkaline phosphatase (ALP) and mRNA for dentin matrix acidic phosphoprotein (DMP1) in tibia of pups from subclinical hypothyroid dams in early postnatal development. Subclinical hypothyroidism (SHT) was induced with propylthiouracil through drinking water (1.5 mg/l) in pregnant Albino Oxford dams from the first day of gravidity and during lactation. Control group was untreated. Five seven-day-old pups from each experimental group were euthanized. All histological examinations were performed on paraffin sections of the proximal tibial growth plate. Histochemistry was used to assess the expression of TRAP, immunohistochemistry for ALP and in situ hybridization for DMP1. The area of TRAP positive signal per 1µm2 and optical density of ALP immunostaining were evaluated using the Image J. The number of DMP1 positive cells was counted for given area using the same software. Level of thyroxine (T4) and triiodothyronine (T3) of dams and pups were decreased (data published). Expression of TRAP and ALP in the proximal tibial growth plate were lower in hypothyroid pups and the number of cells expressing mRNA for DMP1 was decreased. 
As it is known DMP1 and ALP are expressed in osteoblasts with an important role in endochondral ossification while TRAP represents a marker of osteoclasts function and bone resorption, so we can conclude  that maternal SHT in rats leads to delayed osteogenic differentiation and compromised cartilage to bone transition in early infantile period.
PB  - Faculty of Veterinary Medicine, University of Zagreb
C3  - 7th International Congress, Veterinary Science and Profession, Zagreb, October 5th-7th 2017
T1  - Subclinical hypothyroidism in gravide albino oxford rats cause delayed osteogenic differentiation in newborns
SP  - 151
EP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2603
ER  - 

@conference{
author = "Milošević, Ivan and Danilović Luković, Jelena and Lužajić, Tijana and Marković, Danica and Radovanović, Anita and Sourice-Petit, Sophie and Guicheux, Jerome and Kovačević Filipović, Milica",
year = "2017",
abstract = "Thyroid hormones (TH) are indispensable for normal development in mammals. The effects of maternal hypothyroidism on development and function of different organs of their offspring are well known. It is unclear how its subclinical form affects some of these processes, including endochondral ossification during the prenatal and early postnatal development.
The aim of the study was to assess bone formation by defining the expression of tartarate-resistant acid phosphatase (TRAP), alkaline phosphatase (ALP) and mRNA for dentin matrix acidic phosphoprotein (DMP1) in tibia of pups from subclinical hypothyroid dams in early postnatal development. Subclinical hypothyroidism (SHT) was induced with propylthiouracil through drinking water (1.5 mg/l) in pregnant Albino Oxford dams from the first day of gravidity and during lactation. Control group was untreated. Five seven-day-old pups from each experimental group were euthanized. All histological examinations were performed on paraffin sections of the proximal tibial growth plate. Histochemistry was used to assess the expression of TRAP, immunohistochemistry for ALP and in situ hybridization for DMP1. The area of TRAP positive signal per 1µm2 and optical density of ALP immunostaining were evaluated using the Image J. The number of DMP1 positive cells was counted for given area using the same software. Level of thyroxine (T4) and triiodothyronine (T3) of dams and pups were decreased (data published). Expression of TRAP and ALP in the proximal tibial growth plate were lower in hypothyroid pups and the number of cells expressing mRNA for DMP1 was decreased. 
As it is known DMP1 and ALP are expressed in osteoblasts with an important role in endochondral ossification while TRAP represents a marker of osteoclasts function and bone resorption, so we can conclude  that maternal SHT in rats leads to delayed osteogenic differentiation and compromised cartilage to bone transition in early infantile period.",
publisher = "Faculty of Veterinary Medicine, University of Zagreb",
journal = "7th International Congress, Veterinary Science and Profession, Zagreb, October 5th-7th 2017",
title = "Subclinical hypothyroidism in gravide albino oxford rats cause delayed osteogenic differentiation in newborns",
pages = "151-151",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2603"
}

Milošević, I., Danilović Luković, J., Lužajić, T., Marković, D., Radovanović, A., Sourice-Petit, S., Guicheux, J.,& Kovačević Filipović, M.. (2017). Subclinical hypothyroidism in gravide albino oxford rats cause delayed osteogenic differentiation in newborns. in 7th International Congress, Veterinary Science and Profession, Zagreb, October 5th-7th 2017
Faculty of Veterinary Medicine, University of Zagreb., 151-151.
https://hdl.handle.net/21.15107/rcub_veterinar_2603

Milošević I, Danilović Luković J, Lužajić T, Marković D, Radovanović A, Sourice-Petit S, Guicheux J, Kovačević Filipović M. Subclinical hypothyroidism in gravide albino oxford rats cause delayed osteogenic differentiation in newborns. in 7th International Congress, Veterinary Science and Profession, Zagreb, October 5th-7th 2017. 2017;:151-151.
https://hdl.handle.net/21.15107/rcub_veterinar_2603 .

Milošević, Ivan, Danilović Luković, Jelena, Lužajić, Tijana, Marković, Danica, Radovanović, Anita, Sourice-Petit, Sophie, Guicheux, Jerome, Kovačević Filipović, Milica, "Subclinical hypothyroidism in gravide albino oxford rats cause delayed osteogenic differentiation in newborns" in 7th International Congress, Veterinary Science and Profession, Zagreb, October 5th-7th 2017 (2017):151-151,
https://hdl.handle.net/21.15107/rcub_veterinar_2603 .

TY  - JOUR
AU  - Danilović Luković, Jelena
AU  - Korać, Aleksandra
AU  - Milošević, Ivan
AU  - Lužajić, Tijana
AU  - Puškaš, Nela
AU  - Kovačević-Filipović, Milica
AU  - Radovanović, Anita
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2056
AB  - Thyroid hormones (TH) are one of the key factors for normal prenatal development in mammals. Previously, we showed that subclinical maternal hypothyroidism leads to premature atresia of ovarian follicles in female rat offspring in the pre-pubertal and pubertal periods. The influence of decreased concentration of TH on primordial follicles pool formation during neonatal and early infantile period of rat pups was not investigated previously. Maternal hypothyroidism during pregnancy has irreversible negative influence on primordial follicles pool formation and population of resting oocytes in female rat offspring. The study was done on neonatal and early infantile control (n-10) and hypothyroid (n-10) female rat pups derived from control (n-6) and propylthiouracil (PTU) treated pregnant dams (n-6), respectively. Ovaries of all pups were removed and processed for light and transmission electron microscopy (TEM). Number of nests, oogonia and oocytes per nest, primordial, primary, secondary and preantral follicles were determined. Screening for overall calcium presence in ovarian tissue was done using Alizarin red staining. Morphology and volume density of nucleus, mitochondria and smooth endoplasmic reticulum (sER) in the oocytes in primordial follicles was also assessed. Caspase-3 and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), both markers for apoptosis, and proliferating cell nuclear antigen (PCNA) for proliferation were determined in oocytes and granulosa cells in different type of follicles. In neonatal period, ovaries of hypothyroid pups had a decreased number of oogonia, oocytes and nests, an increased number of primordial follicles and a decreased number of primary and secondary follicles, while in early infantile period, increased number of primary, secondary and preantral follicles were found. Alizarin red staining was intense in hypothyroid neonatal rats that also had the highest content of dilated sER. Number of mitochondria with altered morphology in both groups of hypothyroid pups was increased. Apoptosis markers have not shown significant difference between groups but PCNA had an increased expression in the oocytes and granulosa cells in primordial follicles of hypothyroid rats. Light and electron microscopy analysis indicate that previously detected premature ovarian follicular atresia in pre-pubertal and pubertal hypothyroid rats is preceded with premature formation of primordial follicles followed by slight changes on sER and mitochondria in examined oocytes, and increased expression of PCNA.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Micron
T1  - Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach
VL  - 90
SP  - 33
EP  - 42
DO  - 10.1016/j.micron.2016.08.007
ER  - 

@article{
author = "Danilović Luković, Jelena and Korać, Aleksandra and Milošević, Ivan and Lužajić, Tijana and Puškaš, Nela and Kovačević-Filipović, Milica and Radovanović, Anita",
year = "2016",
abstract = "Thyroid hormones (TH) are one of the key factors for normal prenatal development in mammals. Previously, we showed that subclinical maternal hypothyroidism leads to premature atresia of ovarian follicles in female rat offspring in the pre-pubertal and pubertal periods. The influence of decreased concentration of TH on primordial follicles pool formation during neonatal and early infantile period of rat pups was not investigated previously. Maternal hypothyroidism during pregnancy has irreversible negative influence on primordial follicles pool formation and population of resting oocytes in female rat offspring. The study was done on neonatal and early infantile control (n-10) and hypothyroid (n-10) female rat pups derived from control (n-6) and propylthiouracil (PTU) treated pregnant dams (n-6), respectively. Ovaries of all pups were removed and processed for light and transmission electron microscopy (TEM). Number of nests, oogonia and oocytes per nest, primordial, primary, secondary and preantral follicles were determined. Screening for overall calcium presence in ovarian tissue was done using Alizarin red staining. Morphology and volume density of nucleus, mitochondria and smooth endoplasmic reticulum (sER) in the oocytes in primordial follicles was also assessed. Caspase-3 and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), both markers for apoptosis, and proliferating cell nuclear antigen (PCNA) for proliferation were determined in oocytes and granulosa cells in different type of follicles. In neonatal period, ovaries of hypothyroid pups had a decreased number of oogonia, oocytes and nests, an increased number of primordial follicles and a decreased number of primary and secondary follicles, while in early infantile period, increased number of primary, secondary and preantral follicles were found. Alizarin red staining was intense in hypothyroid neonatal rats that also had the highest content of dilated sER. Number of mitochondria with altered morphology in both groups of hypothyroid pups was increased. Apoptosis markers have not shown significant difference between groups but PCNA had an increased expression in the oocytes and granulosa cells in primordial follicles of hypothyroid rats. Light and electron microscopy analysis indicate that previously detected premature ovarian follicular atresia in pre-pubertal and pubertal hypothyroid rats is preceded with premature formation of primordial follicles followed by slight changes on sER and mitochondria in examined oocytes, and increased expression of PCNA.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Micron",
title = "Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach",
volume = "90",
pages = "33-42",
doi = "10.1016/j.micron.2016.08.007"
}

Danilović Luković, J., Korać, A., Milošević, I., Lužajić, T., Puškaš, N., Kovačević-Filipović, M.,& Radovanović, A.. (2016). Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach. in Micron
Pergamon-Elsevier Science Ltd, Oxford., 90, 33-42.
https://doi.org/10.1016/j.micron.2016.08.007

Danilović Luković J, Korać A, Milošević I, Lužajić T, Puškaš N, Kovačević-Filipović M, Radovanović A. Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach. in Micron. 2016;90:33-42.
doi:10.1016/j.micron.2016.08.007 .

Danilović Luković, Jelena, Korać, Aleksandra, Milošević, Ivan, Lužajić, Tijana, Puškaš, Nela, Kovačević-Filipović, Milica, Radovanović, Anita, "Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach" in Micron, 90 (2016):33-42,
https://doi.org/10.1016/j.micron.2016.08.007 . .

TY  - CONF
AU  - Milošević, Ivan
AU  - Lužajić, Tijana
AU  - Danilović Luković, Jelena
AU  - Marković, Danica
AU  - Kovačević Filipović, Milica
AU  - Radovanović, Anita
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2608
AB  - Thyroid hormones (TH) are indispensable for normal development in mammals. The effects of maternal hypothyroidism on development and function of different organs of their offspring are well known. It is unclear how its subclinical form affects some of these processes, including endochondral ossification during the prenatal and early postnatal development.
The aim of the study was to assess bone formation by defining the expression of tartarate-resistant acid phosphatase (TRAP), alkaline phosphatase (ALP) and mRNA for dentin matrix acidic phosphoprotein (DMP1) in tibia of pups from subclinical hypothyroid dams in early postnatal development. Subclinical hypothyroidism (SHT) was induced with propylthiouracil through drinking water (1.5 mg/l) in pregnant Albino Oxford dams from the first day of gravidity and during lactation. Control group was untreated. Five seven-day-old pups from each experimental group were euthanized. All histological examinations were performed on paraffin sections of the proximal tibial growth plate. Histochemistry was used to assess the expression of TRAP, immunohistochemistry for ALP and in situ hybridization for DMP1. The area of TRAP positive signal per 1µm2 and optical density of ALP immunostaining were evaluated using the Image J. The number of DMP1 positive cells was counted for given area using the same software. Level of thyroxine (T4) and triiodothyronine (T3) of dams and pups were decreased (data published). Expression of TRAP and ALP in the proximal tibial growth plate were lower in hypothyroid pups and the number of cells expressing mRNA for DMP1 was decreased. 
As it is known DMP1 and ALP are expressed in osteoblasts with an important role in endochondral ossification while TRAP represents a marker of osteoclasts function and bone resorption, so we can conclude  that maternal SHT in rats leads to delayed osteogenic differentiation and compromised cartilage to bone transition in early infantile period.
C3  - 5th Congress of Serbian Anatomical Society with international participation, Novi Sad
T1  - Uticaj subkliničkog hipotireoidizma majki na hipertrofičnu diferencijaciju hondrocita u proksimalnoj epifiznoj ploči tibije novorođenih pacova starih 7 dana
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2608
ER  - 

@conference{
author = "Milošević, Ivan and Lužajić, Tijana and Danilović Luković, Jelena and Marković, Danica and Kovačević Filipović, Milica and Radovanović, Anita",
year = "2016",
abstract = "Thyroid hormones (TH) are indispensable for normal development in mammals. The effects of maternal hypothyroidism on development and function of different organs of their offspring are well known. It is unclear how its subclinical form affects some of these processes, including endochondral ossification during the prenatal and early postnatal development.
The aim of the study was to assess bone formation by defining the expression of tartarate-resistant acid phosphatase (TRAP), alkaline phosphatase (ALP) and mRNA for dentin matrix acidic phosphoprotein (DMP1) in tibia of pups from subclinical hypothyroid dams in early postnatal development. Subclinical hypothyroidism (SHT) was induced with propylthiouracil through drinking water (1.5 mg/l) in pregnant Albino Oxford dams from the first day of gravidity and during lactation. Control group was untreated. Five seven-day-old pups from each experimental group were euthanized. All histological examinations were performed on paraffin sections of the proximal tibial growth plate. Histochemistry was used to assess the expression of TRAP, immunohistochemistry for ALP and in situ hybridization for DMP1. The area of TRAP positive signal per 1µm2 and optical density of ALP immunostaining were evaluated using the Image J. The number of DMP1 positive cells was counted for given area using the same software. Level of thyroxine (T4) and triiodothyronine (T3) of dams and pups were decreased (data published). Expression of TRAP and ALP in the proximal tibial growth plate were lower in hypothyroid pups and the number of cells expressing mRNA for DMP1 was decreased. 
As it is known DMP1 and ALP are expressed in osteoblasts with an important role in endochondral ossification while TRAP represents a marker of osteoclasts function and bone resorption, so we can conclude  that maternal SHT in rats leads to delayed osteogenic differentiation and compromised cartilage to bone transition in early infantile period.",
journal = "5th Congress of Serbian Anatomical Society with international participation, Novi Sad",
title = "Uticaj subkliničkog hipotireoidizma majki na hipertrofičnu diferencijaciju hondrocita u proksimalnoj epifiznoj ploči tibije novorođenih pacova starih 7 dana",
pages = "96",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2608"
}

Milošević, I., Lužajić, T., Danilović Luković, J., Marković, D., Kovačević Filipović, M.,& Radovanović, A.. (2016). Uticaj subkliničkog hipotireoidizma majki na hipertrofičnu diferencijaciju hondrocita u proksimalnoj epifiznoj ploči tibije novorođenih pacova starih 7 dana. in 5th Congress of Serbian Anatomical Society with international participation, Novi Sad, 96.
https://hdl.handle.net/21.15107/rcub_veterinar_2608

Milošević I, Lužajić T, Danilović Luković J, Marković D, Kovačević Filipović M, Radovanović A. Uticaj subkliničkog hipotireoidizma majki na hipertrofičnu diferencijaciju hondrocita u proksimalnoj epifiznoj ploči tibije novorođenih pacova starih 7 dana. in 5th Congress of Serbian Anatomical Society with international participation, Novi Sad. 2016;:96.
https://hdl.handle.net/21.15107/rcub_veterinar_2608 .

Milošević, Ivan, Lužajić, Tijana, Danilović Luković, Jelena, Marković, Danica, Kovačević Filipović, Milica, Radovanović, Anita, "Uticaj subkliničkog hipotireoidizma majki na hipertrofičnu diferencijaciju hondrocita u proksimalnoj epifiznoj ploči tibije novorođenih pacova starih 7 dana" in 5th Congress of Serbian Anatomical Society with international participation, Novi Sad (2016):96,
https://hdl.handle.net/21.15107/rcub_veterinar_2608 .

TY  - CONF
AU  - Lužajić, Tijana
AU  - Milošević, Ivan
AU  - Danilović Luković, Jelena
AU  - Marković, Danica
AU  - Kovačević Filipović, Milica
AU  - Radovanović, Anita
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2607
AB  - Uvod: Poznato je da su tireoidni hormoni (TH) od esencijalnog značaja za razvoj, funkciju i diferencijaciju svih ćelija u organizmu u toku prenatalnog i postnatalnog života. Prethodna istraživanja dovode u vezu smanjen nivo TH i razvoj reumatoidnog artritisa i drugih oboljenja zglobova, međutim mehanizam nastanka navedenih promena još uvek nije razjašnjen u potpunosti. U sinovijalnim membranama (SM) zglobova zahvaćenih reumatoidnim artritisom, utvrđeno je povećanje broja ćelija koje eksprimiraju PCNA (proliferating cell nuclear antigen). Cilj ovog istraživanja je bio da se definiše uticaj subkliničkog hipotireoidizma majki tokom graviditeta na SM novorođenih jedinki, s obzirom da u dosadašnjim istraživanjima ovaj efekat nije dovoljno proučen.
Materijal i metode: Subklinički hipotireoidizam je kod gravidnih jedinki Albino Oxford pacova prouzrokovan dodavanjem propiltiouracila kroz vodu za piće (1,5 mg/l), počevši od prvog dana graviditeta i tokom laktacije. Kontrolna grupa pacova nije tretirana. Mladunci su žrtvovani sedmog dana nakon rođenja. Histološka ispitivanja sprovedena su na parafinskim presecima debljine 5µm obojenim standardnim hematoksilin/eozin histohemijskim bojenjem, čija je analiza sprovedena na svetlosnom mikroskopu (Olympus BX53). Ekspresija PCNA (Santa Cruz Biotechnology, sc-7907) pokazana je imunohistohemijski, pomoću vizualizacionog kita- Rabbit Specific HRP/DAB (ABC) Detection IHC Kit (ab64261, Abcam, UK). Kvantifikacija ekspresije PCNA izvršena je pomoću softvera Image J. . Prikazan je broj PCNA pozitivnih ćelija u odnosu na 3000 ćelija SM.
Rezultati: Pregledom preseka bojenih hematoksilin/eozinom nisu uočene značajne razlike u građi SM između kontrolne i tretirane grupe. Broj ćelija koje eksprimiraju PCNA u SM tretiranih životinja je značajno veća od broja ćelija u SM kontrolnih životinja. 
Zaključak: Povećana ekspresija PCNA ukazuje na intenziviranu proliferaciju ćelija SM novorođenih pacova starosti 7 dana. Ovaj rezultat može biti osnova za nastavak istraživanja uticaja hipotireoidizma na razviće SM.
C3  - 5th Congress of Serbian Anatomical Society with international participation, Novi Sad
T1  - Uticaj subkliničkog hipotireoidizma majki na razvoj sinovijalne membrane novorođenih pacova starih 7 dana
SP  - 95
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2607
ER  - 

@conference{
author = "Lužajić, Tijana and Milošević, Ivan and Danilović Luković, Jelena and Marković, Danica and Kovačević Filipović, Milica and Radovanović, Anita",
year = "2016",
abstract = "Uvod: Poznato je da su tireoidni hormoni (TH) od esencijalnog značaja za razvoj, funkciju i diferencijaciju svih ćelija u organizmu u toku prenatalnog i postnatalnog života. Prethodna istraživanja dovode u vezu smanjen nivo TH i razvoj reumatoidnog artritisa i drugih oboljenja zglobova, međutim mehanizam nastanka navedenih promena još uvek nije razjašnjen u potpunosti. U sinovijalnim membranama (SM) zglobova zahvaćenih reumatoidnim artritisom, utvrđeno je povećanje broja ćelija koje eksprimiraju PCNA (proliferating cell nuclear antigen). Cilj ovog istraživanja je bio da se definiše uticaj subkliničkog hipotireoidizma majki tokom graviditeta na SM novorođenih jedinki, s obzirom da u dosadašnjim istraživanjima ovaj efekat nije dovoljno proučen.
Materijal i metode: Subklinički hipotireoidizam je kod gravidnih jedinki Albino Oxford pacova prouzrokovan dodavanjem propiltiouracila kroz vodu za piće (1,5 mg/l), počevši od prvog dana graviditeta i tokom laktacije. Kontrolna grupa pacova nije tretirana. Mladunci su žrtvovani sedmog dana nakon rođenja. Histološka ispitivanja sprovedena su na parafinskim presecima debljine 5µm obojenim standardnim hematoksilin/eozin histohemijskim bojenjem, čija je analiza sprovedena na svetlosnom mikroskopu (Olympus BX53). Ekspresija PCNA (Santa Cruz Biotechnology, sc-7907) pokazana je imunohistohemijski, pomoću vizualizacionog kita- Rabbit Specific HRP/DAB (ABC) Detection IHC Kit (ab64261, Abcam, UK). Kvantifikacija ekspresije PCNA izvršena je pomoću softvera Image J. . Prikazan je broj PCNA pozitivnih ćelija u odnosu na 3000 ćelija SM.
Rezultati: Pregledom preseka bojenih hematoksilin/eozinom nisu uočene značajne razlike u građi SM između kontrolne i tretirane grupe. Broj ćelija koje eksprimiraju PCNA u SM tretiranih životinja je značajno veća od broja ćelija u SM kontrolnih životinja. 
Zaključak: Povećana ekspresija PCNA ukazuje na intenziviranu proliferaciju ćelija SM novorođenih pacova starosti 7 dana. Ovaj rezultat može biti osnova za nastavak istraživanja uticaja hipotireoidizma na razviće SM.",
journal = "5th Congress of Serbian Anatomical Society with international participation, Novi Sad",
title = "Uticaj subkliničkog hipotireoidizma majki na razvoj sinovijalne membrane novorođenih pacova starih 7 dana",
pages = "95",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2607"
}

Lužajić, T., Milošević, I., Danilović Luković, J., Marković, D., Kovačević Filipović, M.,& Radovanović, A.. (2016). Uticaj subkliničkog hipotireoidizma majki na razvoj sinovijalne membrane novorođenih pacova starih 7 dana. in 5th Congress of Serbian Anatomical Society with international participation, Novi Sad, 95.
https://hdl.handle.net/21.15107/rcub_veterinar_2607

Lužajić T, Milošević I, Danilović Luković J, Marković D, Kovačević Filipović M, Radovanović A. Uticaj subkliničkog hipotireoidizma majki na razvoj sinovijalne membrane novorođenih pacova starih 7 dana. in 5th Congress of Serbian Anatomical Society with international participation, Novi Sad. 2016;:95.
https://hdl.handle.net/21.15107/rcub_veterinar_2607 .

Lužajić, Tijana, Milošević, Ivan, Danilović Luković, Jelena, Marković, Danica, Kovačević Filipović, Milica, Radovanović, Anita, "Uticaj subkliničkog hipotireoidizma majki na razvoj sinovijalne membrane novorođenih pacova starih 7 dana" in 5th Congress of Serbian Anatomical Society with international participation, Novi Sad (2016):95,
https://hdl.handle.net/21.15107/rcub_veterinar_2607 .

TY  - JOUR
AU  - Danilović-Luković, Jelena
AU  - Korac, Aleksandra
AU  - Milošević, Ivan
AU  - Lužajić, Tijana
AU  - Puskas, Nela
AU  - Kovačević-Filipović, Milica
AU  - Radovanović, Anita
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1428
AB  - Thyroid hormones (TH) are one of the key factors for normal prenatal development in mammals. Previously, we showed that subclinical maternal hypothyroidism leads to premature atresia of ovarian follicles in female rat offspring in the pre-pubertal and pubertal periods. The influence of decreased concentration of TH on primordial follicles pool formation during neonatal and early infantile period of rat pups was not investigated previously. Maternal hypothyroidism during pregnancy has irreversible negative influence on primordial follicles pool formation and population of resting oocytes in female rat offspring. The study was done on neonatal and early infantile control (n-10) and hypothyroid (n-10) female rat pups derived from control (n-6) and propylthiouracil (PTU) treated pregnant dams (n-6), respectively. Ovaries of all pups were removed and processed for light and transmission electron microscopy (TEM). Number of nests, oogonia and oocytes per nest, primordial, primary, secondary and preantral follicles were determined. Screening for overall calcium presence in ovarian tissue was done using Alizarin red staining. Morphology and volume density of nucleus, mitochondria and smooth endoplasmic reticulum (sER) in the oocytes in primordial follicles was also assessed. Caspase-3 and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), both markers for apoptosis, and proliferating cell nuclear antigen (PCNA) for proliferation were determined in oocytes and granulosa cells in different type of follicles. In neonatal period, ovaries of hypothyroid pups had a decreased number of oogonia, oocytes and nests, an increased number of primordial follicles and a decreased number of primary and secondary follicles, while in early infantile period, increased number of primary, secondary and preantral follicles were found. Alizarin red staining was intense in hypothyroid neonatal rats that also had the highest content of dilated sER. Number of mitochondria with altered morphology in both groups of hypothyroid pups was increased. Apoptosis markers have not shown significant difference between groups but PCNA had an increased expression in the oocytes and granulosa cells in primordial follicles of hypothyroid rats. Light and electron microscopy analysis indicate that previously detected premature ovarian follicular atresia in pre-pubertal and pubertal hypothyroid rats is preceded with premature formation of primordial follicles followed by slight changes on sER and mitochondria in examined oocytes, and increased expression of PCNA.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Micron
T1  - Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach
VL  - 90
SP  - 33
EP  - 42
DO  - 10.1016/j.micron.2016.08.007
ER  - 

@article{
author = "Danilović-Luković, Jelena and Korac, Aleksandra and Milošević, Ivan and Lužajić, Tijana and Puskas, Nela and Kovačević-Filipović, Milica and Radovanović, Anita",
year = "2016",
abstract = "Thyroid hormones (TH) are one of the key factors for normal prenatal development in mammals. Previously, we showed that subclinical maternal hypothyroidism leads to premature atresia of ovarian follicles in female rat offspring in the pre-pubertal and pubertal periods. The influence of decreased concentration of TH on primordial follicles pool formation during neonatal and early infantile period of rat pups was not investigated previously. Maternal hypothyroidism during pregnancy has irreversible negative influence on primordial follicles pool formation and population of resting oocytes in female rat offspring. The study was done on neonatal and early infantile control (n-10) and hypothyroid (n-10) female rat pups derived from control (n-6) and propylthiouracil (PTU) treated pregnant dams (n-6), respectively. Ovaries of all pups were removed and processed for light and transmission electron microscopy (TEM). Number of nests, oogonia and oocytes per nest, primordial, primary, secondary and preantral follicles were determined. Screening for overall calcium presence in ovarian tissue was done using Alizarin red staining. Morphology and volume density of nucleus, mitochondria and smooth endoplasmic reticulum (sER) in the oocytes in primordial follicles was also assessed. Caspase-3 and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), both markers for apoptosis, and proliferating cell nuclear antigen (PCNA) for proliferation were determined in oocytes and granulosa cells in different type of follicles. In neonatal period, ovaries of hypothyroid pups had a decreased number of oogonia, oocytes and nests, an increased number of primordial follicles and a decreased number of primary and secondary follicles, while in early infantile period, increased number of primary, secondary and preantral follicles were found. Alizarin red staining was intense in hypothyroid neonatal rats that also had the highest content of dilated sER. Number of mitochondria with altered morphology in both groups of hypothyroid pups was increased. Apoptosis markers have not shown significant difference between groups but PCNA had an increased expression in the oocytes and granulosa cells in primordial follicles of hypothyroid rats. Light and electron microscopy analysis indicate that previously detected premature ovarian follicular atresia in pre-pubertal and pubertal hypothyroid rats is preceded with premature formation of primordial follicles followed by slight changes on sER and mitochondria in examined oocytes, and increased expression of PCNA.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Micron",
title = "Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach",
volume = "90",
pages = "33-42",
doi = "10.1016/j.micron.2016.08.007"
}

Danilović-Luković, J., Korac, A., Milošević, I., Lužajić, T., Puskas, N., Kovačević-Filipović, M.,& Radovanović, A.. (2016). Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach. in Micron
Pergamon-Elsevier Science Ltd, Oxford., 90, 33-42.
https://doi.org/10.1016/j.micron.2016.08.007

Danilović-Luković J, Korac A, Milošević I, Lužajić T, Puskas N, Kovačević-Filipović M, Radovanović A. Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach. in Micron. 2016;90:33-42.
doi:10.1016/j.micron.2016.08.007 .

Danilović-Luković, Jelena, Korac, Aleksandra, Milošević, Ivan, Lužajić, Tijana, Puskas, Nela, Kovačević-Filipović, Milica, Radovanović, Anita, "Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach" in Micron, 90 (2016):33-42,
https://doi.org/10.1016/j.micron.2016.08.007 . .

TY  - JOUR
AU  - Roksandić, Dragutin
AU  - Radovanović, Anita
AU  - Danilović-Luković, Jelena
AU  - Marković, Danica
AU  - Kovačević-Filipović, Milica
AU  - Colić, Miodrag
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1231
AB  - The purpose of this work was to investigate the influence of hypothyroidism on spleen tissue morphology and immune cell density in fourteen-day-old juvenile rats. Hypothyroidism in pups (n=10) was induced by administration of propylthiouracil (PTU) in drinking water (1.5 mg/L) to their mothers during pregnancy and period of lactation. Fourteen-day-old pups were sacrificed and the thyroid-stimulating hormone (TSH) serum concentration and thyroid activation index (Ia) were determined. Increased serum level of TSH and increased Ia showed that pups from PTU treated mothers were hypothyroid. White and red spleen pulp, marginal zone and connective tissue volume density has been assessed by using the stereological method. Using immunohistochemistry, the present CD3+ T lymphocytes, CD45RA+ B lymphocytes and CD68+ macrophages were quantified. A significant reduction of volume density of the periarteriolar lymphocyte sheath (Vv(PALS)) and lymphatic follicles (Vvf) due to depletion of T and B lymphocytes respectively, was observed in the spleens of hypothyroid pups compared to controls. The volume density of the red pulp (Vvrp), marginal zone (Vvmz) and connective tissue (Vvct) was increased, as well as the number of CD68+ macrophages in the spleens of hypothyroid pups compared to controls. These results indicate that thyroid hormones might be important for normal development of both, specific and innate immune cells in the spleen during prenatal and early postnatal period.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats
VL  - 65
IS  - 2
SP  - 246
EP  - 259
DO  - 10.1515/acve-2015-0021
ER  - 

@article{
author = "Roksandić, Dragutin and Radovanović, Anita and Danilović-Luković, Jelena and Marković, Danica and Kovačević-Filipović, Milica and Colić, Miodrag",
year = "2015",
abstract = "The purpose of this work was to investigate the influence of hypothyroidism on spleen tissue morphology and immune cell density in fourteen-day-old juvenile rats. Hypothyroidism in pups (n=10) was induced by administration of propylthiouracil (PTU) in drinking water (1.5 mg/L) to their mothers during pregnancy and period of lactation. Fourteen-day-old pups were sacrificed and the thyroid-stimulating hormone (TSH) serum concentration and thyroid activation index (Ia) were determined. Increased serum level of TSH and increased Ia showed that pups from PTU treated mothers were hypothyroid. White and red spleen pulp, marginal zone and connective tissue volume density has been assessed by using the stereological method. Using immunohistochemistry, the present CD3+ T lymphocytes, CD45RA+ B lymphocytes and CD68+ macrophages were quantified. A significant reduction of volume density of the periarteriolar lymphocyte sheath (Vv(PALS)) and lymphatic follicles (Vvf) due to depletion of T and B lymphocytes respectively, was observed in the spleens of hypothyroid pups compared to controls. The volume density of the red pulp (Vvrp), marginal zone (Vvmz) and connective tissue (Vvct) was increased, as well as the number of CD68+ macrophages in the spleens of hypothyroid pups compared to controls. These results indicate that thyroid hormones might be important for normal development of both, specific and innate immune cells in the spleen during prenatal and early postnatal period.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats",
volume = "65",
number = "2",
pages = "246-259",
doi = "10.1515/acve-2015-0021"
}

Roksandić, D., Radovanović, A., Danilović-Luković, J., Marković, D., Kovačević-Filipović, M.,& Colić, M.. (2015). Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(2), 246-259.
https://doi.org/10.1515/acve-2015-0021

Roksandić D, Radovanović A, Danilović-Luković J, Marković D, Kovačević-Filipović M, Colić M. Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats. in Acta Veterinaria-Beograd. 2015;65(2):246-259.
doi:10.1515/acve-2015-0021 .

Roksandić, Dragutin, Radovanović, Anita, Danilović-Luković, Jelena, Marković, Danica, Kovačević-Filipović, Milica, Colić, Miodrag, "Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats" in Acta Veterinaria-Beograd, 65, no. 2 (2015):246-259,
https://doi.org/10.1515/acve-2015-0021 . .

TY  - CONF
AU  - Lužajić, Tijana
AU  - Katić, M.
AU  - Danilović Luković, Jelena
AU  - Blond, B.
AU  - Bogdanović, Dragan
AU  - Kovačević Filipović, Milica
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2600
PB  - Serbian Buiatric's Association, Faculty of Veterinary Medicine, University of Belgrade
C3  - XIII Middle European Buiatric's Congress, Belgrade, June 5-8, 2013
T1  - Prepartal platelet count and fibrinogen concentration in dairy cows with and without retained placenta
SP  - 437
EP  - 441
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2600
ER  - 

@conference{
author = "Lužajić, Tijana and Katić, M. and Danilović Luković, Jelena and Blond, B. and Bogdanović, Dragan and Kovačević Filipović, Milica",
year = "2013",
publisher = "Serbian Buiatric's Association, Faculty of Veterinary Medicine, University of Belgrade",
journal = "XIII Middle European Buiatric's Congress, Belgrade, June 5-8, 2013",
title = "Prepartal platelet count and fibrinogen concentration in dairy cows with and without retained placenta",
pages = "437-441",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2600"
}

Lužajić, T., Katić, M., Danilović Luković, J., Blond, B., Bogdanović, D.,& Kovačević Filipović, M.. (2013). Prepartal platelet count and fibrinogen concentration in dairy cows with and without retained placenta. in XIII Middle European Buiatric's Congress, Belgrade, June 5-8, 2013
Serbian Buiatric's Association, Faculty of Veterinary Medicine, University of Belgrade., 437-441.
https://hdl.handle.net/21.15107/rcub_veterinar_2600

Lužajić T, Katić M, Danilović Luković J, Blond B, Bogdanović D, Kovačević Filipović M. Prepartal platelet count and fibrinogen concentration in dairy cows with and without retained placenta. in XIII Middle European Buiatric's Congress, Belgrade, June 5-8, 2013. 2013;:437-441.
https://hdl.handle.net/21.15107/rcub_veterinar_2600 .

Lužajić, Tijana, Katić, M., Danilović Luković, Jelena, Blond, B., Bogdanović, Dragan, Kovačević Filipović, Milica, "Prepartal platelet count and fibrinogen concentration in dairy cows with and without retained placenta" in XIII Middle European Buiatric's Congress, Belgrade, June 5-8, 2013 (2013):437-441,
https://hdl.handle.net/21.15107/rcub_veterinar_2600 .

TY  - CONF
AU  - Danilović Luković, Jelena
AU  - Katić, Marko
AU  - Lužajić, Tijana
AU  - Blond, B
AU  - Kovačević Filipović, Milica
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2591
C3  - XIII Srednjeevropski Kongres Bujatričara/XIII Middle European Buiatric's Congress, Beograd
T1  - Hematology and biochemistry parametars in neonatal calves with low iron levels
SP  - 284
EP  - 288
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2591
ER  - 

@conference{
author = "Danilović Luković, Jelena and Katić, Marko and Lužajić, Tijana and Blond, B and Kovačević Filipović, Milica",
year = "2013",
journal = "XIII Srednjeevropski Kongres Bujatričara/XIII Middle European Buiatric's Congress, Beograd",
title = "Hematology and biochemistry parametars in neonatal calves with low iron levels",
pages = "284-288",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2591"
}

Danilović Luković, J., Katić, M., Lužajić, T., Blond, B.,& Kovačević Filipović, M.. (2013). Hematology and biochemistry parametars in neonatal calves with low iron levels. in XIII Srednjeevropski Kongres Bujatričara/XIII Middle European Buiatric's Congress, Beograd, 284-288.
https://hdl.handle.net/21.15107/rcub_veterinar_2591

Danilović Luković J, Katić M, Lužajić T, Blond B, Kovačević Filipović M. Hematology and biochemistry parametars in neonatal calves with low iron levels. in XIII Srednjeevropski Kongres Bujatričara/XIII Middle European Buiatric's Congress, Beograd. 2013;:284-288.
https://hdl.handle.net/21.15107/rcub_veterinar_2591 .

Danilović Luković, Jelena, Katić, Marko, Lužajić, Tijana, Blond, B, Kovačević Filipović, Milica, "Hematology and biochemistry parametars in neonatal calves with low iron levels" in XIII Srednjeevropski Kongres Bujatričara/XIII Middle European Buiatric's Congress, Beograd (2013):284-288,
https://hdl.handle.net/21.15107/rcub_veterinar_2591 .