TY  - JOUR
AU  - Jakovljević, Goran
AU  - Lazarević, Miodrag
AU  - Mirilović, Milorad
AU  - Milovanović, Aleksandar
AU  - Apić, Jelena
AU  - Šefer, Dragan
AU  - Nedić, Svetlana
AU  - Vakanjac, Slobodanka
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2267
AB  - This study aimed to assess the influence of different concentrations of zinc (Zn), lead (Pb), mercury (Hg) and cadmium (Cd) found in the feed and water provided to bulls on the concentrations of these metals in cryopreserved bull semen, and to determine their influence onto semen quality parameters. Correlations between heavy metal concentrations in the semen and the quality parameters of semen as estimated by computer-assisted sperm analysis (CASA) and flow cytometry (FC) methods were determined. A total of 40 cryopreserved semen samples originating from bulls housed in 4 different centers for artificial insemination (A, B, C and D) were examined, making a total of 160 samples. The concentrations of metals and semen quality parameters were determined in cryopreserved semen of 10 bulls from each center, namely 4 samples from each bull. Concentrations of Zn, Pb, Hg and Cd in hay and concentrated feed were within the allowed limits as proposed by the National Research Council (NRC, 2000). A strong negative correlation was detected between curvilinear velocity (VCL) and Zn concentrations in the semen (P < 0.01; r = -0.772) in group D, and a positive correlation of VCL with Pb concentrations (P < 0.05 and r = 0.718) in group B. Mercury concentrations in cryopreserved semen correlated negatively to the percent of live sperm cells with intact acrosomes (V/IA: P < 0.05; r = -0.640) and positively with the percent of dead sperm cells with damaged acrosomes (D/DA: P < 0.01; r = 0.766) in group D. This finding confirms the hypothesis that Hg, even at low concentrations, may cause acrosome damage.
AB  - Cilj rada bio je da se ispita uticaj različitih koncentracija cinka (Zn), olova (Pb), žive
(Hg) i kadmijuma (Cd) iz hrane i vode na koncentracije ovih metala u duboko zamrznutom semenu nakon otapanja i da se ispita njihov uticaj na parametre kvaliteta
semena. Korelacije između koncentracije teških metala u semenu i parametra kvaliteta semena su određivane nakon ispitivanja semena kompjuterski asistiranom analizom
semena (eng. computer-assisted sperm analysis - CASA) i protočnom citometrijom
(eng. flow cytometry - FC).
Iz 4 različita centra za veštačko osemenjavanje (A, B, C i D) ispitivano je po 40 uzoraka duboko zamrznutog semena bikova, što čini ukupno 160 uzoraka. Koncentracije
metala i parametri kvaliteta semena su određivani u duboko zamrznutom semenu od
10 bikova iz svakog centra, odnosno 4 uzorka od svakog bika. Koncentracije Zn, Pb,
Hg i Cd u senu i koncentrovanim hranivima bile su ispod propisanih dozvoljenih
vrednosti Nacionalnog Istraživačkog Veća (eng. National Research Council - NRC,
2000). Jaka negativna korelacija uočena je između krivolinijske brzine spermatozoida
(eng. curvilinear velocity - VCL) i koncentracije Zn u semenu (P < 0.01; r = -0.772) u
grupi D, i pozitivna korelacija između VCL i koncentracije Pb (P < 0.05 and r = 0.718)
u grupi B. Koncentracija žive u duboko zamrznutom semenu negativno je korelirala
sa procentom živih spermatozoida sa neoštećenim akrozomom (eng. live sperm cells
with intact acrosomes - V/IA: P < 0.05; r = -0.640) i pozitivno sa procentom mrtvih
spermatozoida sa oštećenim akrozomom (eng. dead sperm cells with damaged acrosomes - D/DA: P < 0.01; r = 0.766) u grupi D. Ovi rezultati potvrđuju hipotezu da Hg,
čak i u niskim koncenracijama može da prouzrokuje oštećenje akrozoma.
PB  - Fakultet veterinarske medicine
T2  - Acta veterinaria-Beograd
T1  - The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen
T1  - Uticaj cinka i teških metala iz hrane i vode na kvalitet duboko zamrznutog semena bikova
VL  - 71
IS  - 3
SP  - 326
EP  - 343
DO  - 10.2478/acve-2021-0028
ER  - 

@article{
author = "Jakovljević, Goran and Lazarević, Miodrag and Mirilović, Milorad and Milovanović, Aleksandar and Apić, Jelena and Šefer, Dragan and Nedić, Svetlana and Vakanjac, Slobodanka",
year = "2021",
abstract = "This study aimed to assess the influence of different concentrations of zinc (Zn), lead (Pb), mercury (Hg) and cadmium (Cd) found in the feed and water provided to bulls on the concentrations of these metals in cryopreserved bull semen, and to determine their influence onto semen quality parameters. Correlations between heavy metal concentrations in the semen and the quality parameters of semen as estimated by computer-assisted sperm analysis (CASA) and flow cytometry (FC) methods were determined. A total of 40 cryopreserved semen samples originating from bulls housed in 4 different centers for artificial insemination (A, B, C and D) were examined, making a total of 160 samples. The concentrations of metals and semen quality parameters were determined in cryopreserved semen of 10 bulls from each center, namely 4 samples from each bull. Concentrations of Zn, Pb, Hg and Cd in hay and concentrated feed were within the allowed limits as proposed by the National Research Council (NRC, 2000). A strong negative correlation was detected between curvilinear velocity (VCL) and Zn concentrations in the semen (P < 0.01; r = -0.772) in group D, and a positive correlation of VCL with Pb concentrations (P < 0.05 and r = 0.718) in group B. Mercury concentrations in cryopreserved semen correlated negatively to the percent of live sperm cells with intact acrosomes (V/IA: P < 0.05; r = -0.640) and positively with the percent of dead sperm cells with damaged acrosomes (D/DA: P < 0.01; r = 0.766) in group D. This finding confirms the hypothesis that Hg, even at low concentrations, may cause acrosome damage., Cilj rada bio je da se ispita uticaj različitih koncentracija cinka (Zn), olova (Pb), žive
(Hg) i kadmijuma (Cd) iz hrane i vode na koncentracije ovih metala u duboko zamrznutom semenu nakon otapanja i da se ispita njihov uticaj na parametre kvaliteta
semena. Korelacije između koncentracije teških metala u semenu i parametra kvaliteta semena su određivane nakon ispitivanja semena kompjuterski asistiranom analizom
semena (eng. computer-assisted sperm analysis - CASA) i protočnom citometrijom
(eng. flow cytometry - FC).
Iz 4 različita centra za veštačko osemenjavanje (A, B, C i D) ispitivano je po 40 uzoraka duboko zamrznutog semena bikova, što čini ukupno 160 uzoraka. Koncentracije
metala i parametri kvaliteta semena su određivani u duboko zamrznutom semenu od
10 bikova iz svakog centra, odnosno 4 uzorka od svakog bika. Koncentracije Zn, Pb,
Hg i Cd u senu i koncentrovanim hranivima bile su ispod propisanih dozvoljenih
vrednosti Nacionalnog Istraživačkog Veća (eng. National Research Council - NRC,
2000). Jaka negativna korelacija uočena je između krivolinijske brzine spermatozoida
(eng. curvilinear velocity - VCL) i koncentracije Zn u semenu (P < 0.01; r = -0.772) u
grupi D, i pozitivna korelacija između VCL i koncentracije Pb (P < 0.05 and r = 0.718)
u grupi B. Koncentracija žive u duboko zamrznutom semenu negativno je korelirala
sa procentom živih spermatozoida sa neoštećenim akrozomom (eng. live sperm cells
with intact acrosomes - V/IA: P < 0.05; r = -0.640) i pozitivno sa procentom mrtvih
spermatozoida sa oštećenim akrozomom (eng. dead sperm cells with damaged acrosomes - D/DA: P < 0.01; r = 0.766) u grupi D. Ovi rezultati potvrđuju hipotezu da Hg,
čak i u niskim koncenracijama može da prouzrokuje oštećenje akrozoma.",
publisher = "Fakultet veterinarske medicine",
journal = "Acta veterinaria-Beograd",
title = "The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen, Uticaj cinka i teških metala iz hrane i vode na kvalitet duboko zamrznutog semena bikova",
volume = "71",
number = "3",
pages = "326-343",
doi = "10.2478/acve-2021-0028"
}

Jakovljević, G., Lazarević, M., Mirilović, M., Milovanović, A., Apić, J., Šefer, D., Nedić, S.,& Vakanjac, S.. (2021). The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen. in Acta veterinaria-Beograd
Fakultet veterinarske medicine., 71(3), 326-343.
https://doi.org/10.2478/acve-2021-0028

Jakovljević G, Lazarević M, Mirilović M, Milovanović A, Apić J, Šefer D, Nedić S, Vakanjac S. The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen. in Acta veterinaria-Beograd. 2021;71(3):326-343.
doi:10.2478/acve-2021-0028 .

Jakovljević, Goran, Lazarević, Miodrag, Mirilović, Milorad, Milovanović, Aleksandar, Apić, Jelena, Šefer, Dragan, Nedić, Svetlana, Vakanjac, Slobodanka, "The influence of zinc and heavy metals in feed and water on the quality of cryopreserved bull semen" in Acta veterinaria-Beograd, 71, no. 3 (2021):326-343,
https://doi.org/10.2478/acve-2021-0028 . .

TY  - JOUR
AU  - Barna, Tomislav
AU  - Apić, Jelena
AU  - Milovanović, Aleksandar
AU  - Maksimović, Nevena
AU  - Mašić, Aleksandar
AU  - Lazarević, Mmarina
AU  - Pavlović, Miloš
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2247
AB  - The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA), cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ± 100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%) in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters, as well as ratio of live sperm cells population with damaged acrosome. © 2021 TÜBİTAK.
PB  - Scientific Technical Research Council Turkey-Tubitak, Ankara
T2  - Turkish Journal of Veterinary and Animal Sciences
T1  - Quality and fertility of extended boar semen after prolonged transport
VL  - 45
IS  - 5
SP  - 920
EP  - 929
DO  - 10.3906/VET-2012-63
ER  - 

@article{
author = "Barna, Tomislav and Apić, Jelena and Milovanović, Aleksandar and Maksimović, Nevena and Mašić, Aleksandar and Lazarević, Mmarina and Pavlović, Miloš",
year = "2021",
abstract = "The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA), cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ± 100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%) in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters, as well as ratio of live sperm cells population with damaged acrosome. © 2021 TÜBİTAK.",
publisher = "Scientific Technical Research Council Turkey-Tubitak, Ankara",
journal = "Turkish Journal of Veterinary and Animal Sciences",
title = "Quality and fertility of extended boar semen after prolonged transport",
volume = "45",
number = "5",
pages = "920-929",
doi = "10.3906/VET-2012-63"
}

Barna, T., Apić, J., Milovanović, A., Maksimović, N., Mašić, A., Lazarević, M.,& Pavlović, M.. (2021). Quality and fertility of extended boar semen after prolonged transport. in Turkish Journal of Veterinary and Animal Sciences
Scientific Technical Research Council Turkey-Tubitak, Ankara., 45(5), 920-929.
https://doi.org/10.3906/VET-2012-63

Barna T, Apić J, Milovanović A, Maksimović N, Mašić A, Lazarević M, Pavlović M. Quality and fertility of extended boar semen after prolonged transport. in Turkish Journal of Veterinary and Animal Sciences. 2021;45(5):920-929.
doi:10.3906/VET-2012-63 .

Barna, Tomislav, Apić, Jelena, Milovanović, Aleksandar, Maksimović, Nevena, Mašić, Aleksandar, Lazarević, Mmarina, Pavlović, Miloš, "Quality and fertility of extended boar semen after prolonged transport" in Turkish Journal of Veterinary and Animal Sciences, 45, no. 5 (2021):920-929,
https://doi.org/10.3906/VET-2012-63 . .

TY  - JOUR
AU  - Samardžija, Marko
AU  - Lojkić, Martina
AU  - Maćešić, Nino
AU  - Valpotić, Hrvoje
AU  - Butković, Ivan
AU  - Šavorić, Juraj
AU  - Žura Žaja, Ivona
AU  - Leiner, Denis
AU  - Đuričić, Dražen
AU  - Marković, Franjo
AU  - Kočila, Predrag
AU  - Vidas, Željko
AU  - Gerenčer, Marijan
AU  - Kaštelan, Andrija
AU  - Milovanović, Aleksandar
AU  - Lazarević, Miodrag
AU  - Rukavina, Daniel
AU  - Valpotić, Ivica
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1967
AB  - Literally, reproductive immunology was born in bovine on-farm reproduction where seminal experiments intended for developing methods for embryo transfer in cattle were performed. Actually, these experiments led to two of major concepts and fundamental principles of reproductive immunology using the bovine species as a model for biomedical research, namely the concept of acquired immunological tolerance and the paradox of the semiallogeneic bovine foetus whereby such organism can develop within an immunologically competent host. Peter Medawar, a scientist who together with Frank Macfarlande Burnet shared the 1960 Nobel Prize in physiology or medicine for discovery of acquired immunological tolerance, while studying dizygotic cattle twins, thereby giving birth to reproductive immunology. Also, these findings significantly influenced development of organ transplants and showed that using farm animals as models for studying transplantation immunology had general relevance for mammalian biology and health including those of humans. However, the interest for further research of the fascinating maternal immune influences on pregnancy and perinatal outcomes and of the prevention and treatment of immunologically mediated reproductive disorders in viviparous mammals of veterinary relevance by veterinary immunologists and reproductive clinicians have been very scarce regarding the application of nonspecific immunomodulatory agents for prevention and treatment of subfertility and infertility in pigs and cattle, but still broadening knowledge in this area and hold great potential for improving such therapy in the future. The aim of the current overview is to provide up-to-date information and explaining/translating relevant immunology phenomena into veterinary practice for specialists and scientists/clinicians in reproduction of animals.
PB  - Taylor & Francis
T2  - Veterinary Quarterly
T1  - Reproductive immunology in viviparous mammals: evolutionary paradox of interactions among immune mechanisms and autologous or allogeneic gametes and semiallogeneic foetuses
VL  - 40
IS  - 1
SP  - 353
EP  - 383
DO  - 10.1080/01652176.2020.1852336
ER  - 

@article{
author = "Samardžija, Marko and Lojkić, Martina and Maćešić, Nino and Valpotić, Hrvoje and Butković, Ivan and Šavorić, Juraj and Žura Žaja, Ivona and Leiner, Denis and Đuričić, Dražen and Marković, Franjo and Kočila, Predrag and Vidas, Željko and Gerenčer, Marijan and Kaštelan, Andrija and Milovanović, Aleksandar and Lazarević, Miodrag and Rukavina, Daniel and Valpotić, Ivica",
year = "2020",
abstract = "Literally, reproductive immunology was born in bovine on-farm reproduction where seminal experiments intended for developing methods for embryo transfer in cattle were performed. Actually, these experiments led to two of major concepts and fundamental principles of reproductive immunology using the bovine species as a model for biomedical research, namely the concept of acquired immunological tolerance and the paradox of the semiallogeneic bovine foetus whereby such organism can develop within an immunologically competent host. Peter Medawar, a scientist who together with Frank Macfarlande Burnet shared the 1960 Nobel Prize in physiology or medicine for discovery of acquired immunological tolerance, while studying dizygotic cattle twins, thereby giving birth to reproductive immunology. Also, these findings significantly influenced development of organ transplants and showed that using farm animals as models for studying transplantation immunology had general relevance for mammalian biology and health including those of humans. However, the interest for further research of the fascinating maternal immune influences on pregnancy and perinatal outcomes and of the prevention and treatment of immunologically mediated reproductive disorders in viviparous mammals of veterinary relevance by veterinary immunologists and reproductive clinicians have been very scarce regarding the application of nonspecific immunomodulatory agents for prevention and treatment of subfertility and infertility in pigs and cattle, but still broadening knowledge in this area and hold great potential for improving such therapy in the future. The aim of the current overview is to provide up-to-date information and explaining/translating relevant immunology phenomena into veterinary practice for specialists and scientists/clinicians in reproduction of animals.",
publisher = "Taylor & Francis",
journal = "Veterinary Quarterly",
title = "Reproductive immunology in viviparous mammals: evolutionary paradox of interactions among immune mechanisms and autologous or allogeneic gametes and semiallogeneic foetuses",
volume = "40",
number = "1",
pages = "353-383",
doi = "10.1080/01652176.2020.1852336"
}

Samardžija, M., Lojkić, M., Maćešić, N., Valpotić, H., Butković, I., Šavorić, J., Žura Žaja, I., Leiner, D., Đuričić, D., Marković, F., Kočila, P., Vidas, Ž., Gerenčer, M., Kaštelan, A., Milovanović, A., Lazarević, M., Rukavina, D.,& Valpotić, I.. (2020). Reproductive immunology in viviparous mammals: evolutionary paradox of interactions among immune mechanisms and autologous or allogeneic gametes and semiallogeneic foetuses. in Veterinary Quarterly
Taylor & Francis., 40(1), 353-383.
https://doi.org/10.1080/01652176.2020.1852336

Samardžija M, Lojkić M, Maćešić N, Valpotić H, Butković I, Šavorić J, Žura Žaja I, Leiner D, Đuričić D, Marković F, Kočila P, Vidas Ž, Gerenčer M, Kaštelan A, Milovanović A, Lazarević M, Rukavina D, Valpotić I. Reproductive immunology in viviparous mammals: evolutionary paradox of interactions among immune mechanisms and autologous or allogeneic gametes and semiallogeneic foetuses. in Veterinary Quarterly. 2020;40(1):353-383.
doi:10.1080/01652176.2020.1852336 .

Samardžija, Marko, Lojkić, Martina, Maćešić, Nino, Valpotić, Hrvoje, Butković, Ivan, Šavorić, Juraj, Žura Žaja, Ivona, Leiner, Denis, Đuričić, Dražen, Marković, Franjo, Kočila, Predrag, Vidas, Željko, Gerenčer, Marijan, Kaštelan, Andrija, Milovanović, Aleksandar, Lazarević, Miodrag, Rukavina, Daniel, Valpotić, Ivica, "Reproductive immunology in viviparous mammals: evolutionary paradox of interactions among immune mechanisms and autologous or allogeneic gametes and semiallogeneic foetuses" in Veterinary Quarterly, 40, no. 1 (2020):353-383,
https://doi.org/10.1080/01652176.2020.1852336 . .

TY  - JOUR
AU  - Samardžija, Marko
AU  - Lojkić, Martina
AU  - Maćešić, Nino
AU  - Valpotić, Hrvoje
AU  - Butković, Ivan
AU  - Šavorić, Juraj
AU  - Žura Žaja, Ivona
AU  - Leiner, Denis
AU  - Đuričić, Dražen
AU  - Marković, Franjo
AU  - Kočila, Predrag
AU  - Vidas, Željko
AU  - Gerenčer, Marijan
AU  - Kaštelan, Andrija
AU  - Milovanović, Aleksandar
AU  - Lazarević, Miodrag
AU  - Rukavina, Daniel
AU  - Valpotić, Ivica
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2052
AB  - In the original manuscript the affiliations for the authors M. Gerencer, A. Kastelan, A. Milovanovic, M. Lazarevic, D. Rukavina, I. Valpotic are set incorrectly. This has now been corrected as shown below. M. Samardzijaa , M. Lojkica , N. Macesica , H. Valpoticb , I. Butkovica , J. Savorica , I. Zura  Zaja  c , D. Leinerd , D. Duri - cice , F. Markovicf , P. Kocilag , Z. Vidas  h , M. Gerencerl , A. Kastelani , A. Milovanovicj , M. Lazarevick , D. Rukavinai and I. Valpotic m a Clinic for Obstetrics and Reproduction of Animals, Veterinary Faculty University of Zagreb, Zagreb, Croatia; b Department for Animal Nutrition and Dietetics, Veterinary Faculty University of Zagreb, Zagreb, Croatia; c Department for Physiology and Radiobiology, Veterinary Faculty University of Zagreb, Zagreb, Croatia; d Department of Anatomy, Histology and Embriology, Veterinary Faculty University of Zagreb, Zagreb, Croatia; e Veterinary Practice, Durd - - evac, Croatia; f Belupo d.d. Danica, Koprivnica, Croatia; g Animal Feed Factory, Cakovec, Croatia; h Faculty of Medicine, Department of Urology, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia; i Croatian Academy of Sciences and Arts, Croatian Academy of Sciences and Arts, Zagreb, Croatia; j Department of Reproduction, Veterinary Scientific Institute, Novi Sad, Serbia; k Department for Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia; l Department of Cellular Immunology, Baxter Hyland Immuno, Vienna, Austria; mDepartment of Biology, Veterinary Faculty University of Zagreb, Zagreb, Croatia
PB  - Taylor & Francis
T2  - Veterinary Quarterly
T1  - Erratum: Samardžija, M.; Lojkić, M.; Maćešić, N.; Valpotić, H.; Butković, I.; Šavorić, J.; Žura Žaja, I.; Leiner, D.; Đuričić, D.; Marković, F.; Kočila, P.; Vidas, Ž.; Gerenčer, M.; Kaštelan, A.; Milovanović, A.; Lazarević, M.; Rukavina, D.; Valpotić, I. Reproductive Immunology in Viviparous Mammals: Evolutionary Paradox of Interactions among Immune Mechanisms and Autologous or Allogeneic Gametes and Semiallogeneic Foetuses. Veterinary Quarterly 2020, 40 (1), 353–383. https://doi.org/10.1080/01652176.2020.1852336.
VL  - 41
IS  - 1
SP  - 152
EP  - 152
DO  - 10.1080/01652176.2020.1852336
ER  - 

@article{
author = "Samardžija, Marko and Lojkić, Martina and Maćešić, Nino and Valpotić, Hrvoje and Butković, Ivan and Šavorić, Juraj and Žura Žaja, Ivona and Leiner, Denis and Đuričić, Dražen and Marković, Franjo and Kočila, Predrag and Vidas, Željko and Gerenčer, Marijan and Kaštelan, Andrija and Milovanović, Aleksandar and Lazarević, Miodrag and Rukavina, Daniel and Valpotić, Ivica",
year = "2020",
abstract = "In the original manuscript the affiliations for the authors M. Gerencer, A. Kastelan, A. Milovanovic, M. Lazarevic, D. Rukavina, I. Valpotic are set incorrectly. This has now been corrected as shown below. M. Samardzijaa , M. Lojkica , N. Macesica , H. Valpoticb , I. Butkovica , J. Savorica , I. Zura  Zaja  c , D. Leinerd , D. Duri - cice , F. Markovicf , P. Kocilag , Z. Vidas  h , M. Gerencerl , A. Kastelani , A. Milovanovicj , M. Lazarevick , D. Rukavinai and I. Valpotic m a Clinic for Obstetrics and Reproduction of Animals, Veterinary Faculty University of Zagreb, Zagreb, Croatia; b Department for Animal Nutrition and Dietetics, Veterinary Faculty University of Zagreb, Zagreb, Croatia; c Department for Physiology and Radiobiology, Veterinary Faculty University of Zagreb, Zagreb, Croatia; d Department of Anatomy, Histology and Embriology, Veterinary Faculty University of Zagreb, Zagreb, Croatia; e Veterinary Practice, Durd - - evac, Croatia; f Belupo d.d. Danica, Koprivnica, Croatia; g Animal Feed Factory, Cakovec, Croatia; h Faculty of Medicine, Department of Urology, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia; i Croatian Academy of Sciences and Arts, Croatian Academy of Sciences and Arts, Zagreb, Croatia; j Department of Reproduction, Veterinary Scientific Institute, Novi Sad, Serbia; k Department for Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia; l Department of Cellular Immunology, Baxter Hyland Immuno, Vienna, Austria; mDepartment of Biology, Veterinary Faculty University of Zagreb, Zagreb, Croatia",
publisher = "Taylor & Francis",
journal = "Veterinary Quarterly",
title = "Erratum: Samardžija, M.; Lojkić, M.; Maćešić, N.; Valpotić, H.; Butković, I.; Šavorić, J.; Žura Žaja, I.; Leiner, D.; Đuričić, D.; Marković, F.; Kočila, P.; Vidas, Ž.; Gerenčer, M.; Kaštelan, A.; Milovanović, A.; Lazarević, M.; Rukavina, D.; Valpotić, I. Reproductive Immunology in Viviparous Mammals: Evolutionary Paradox of Interactions among Immune Mechanisms and Autologous or Allogeneic Gametes and Semiallogeneic Foetuses. Veterinary Quarterly 2020, 40 (1), 353–383. https://doi.org/10.1080/01652176.2020.1852336.",
volume = "41",
number = "1",
pages = "152-152",
doi = "10.1080/01652176.2020.1852336"
}

Samardžija, M., Lojkić, M., Maćešić, N., Valpotić, H., Butković, I., Šavorić, J., Žura Žaja, I., Leiner, D., Đuričić, D., Marković, F., Kočila, P., Vidas, Ž., Gerenčer, M., Kaštelan, A., Milovanović, A., Lazarević, M., Rukavina, D.,& Valpotić, I.. (2020). Erratum: Samardžija, M.; Lojkić, M.; Maćešić, N.; Valpotić, H.; Butković, I.; Šavorić, J.; Žura Žaja, I.; Leiner, D.; Đuričić, D.; Marković, F.; Kočila, P.; Vidas, Ž.; Gerenčer, M.; Kaštelan, A.; Milovanović, A.; Lazarević, M.; Rukavina, D.; Valpotić, I. Reproductive Immunology in Viviparous Mammals: Evolutionary Paradox of Interactions among Immune Mechanisms and Autologous or Allogeneic Gametes and Semiallogeneic Foetuses. Veterinary Quarterly 2020, 40 (1), 353–383. https://doi.org/10.1080/01652176.2020.1852336.. in Veterinary Quarterly
Taylor & Francis., 41(1), 152-152.
https://doi.org/10.1080/01652176.2020.1852336

Samardžija M, Lojkić M, Maćešić N, Valpotić H, Butković I, Šavorić J, Žura Žaja I, Leiner D, Đuričić D, Marković F, Kočila P, Vidas Ž, Gerenčer M, Kaštelan A, Milovanović A, Lazarević M, Rukavina D, Valpotić I. Erratum: Samardžija, M.; Lojkić, M.; Maćešić, N.; Valpotić, H.; Butković, I.; Šavorić, J.; Žura Žaja, I.; Leiner, D.; Đuričić, D.; Marković, F.; Kočila, P.; Vidas, Ž.; Gerenčer, M.; Kaštelan, A.; Milovanović, A.; Lazarević, M.; Rukavina, D.; Valpotić, I. Reproductive Immunology in Viviparous Mammals: Evolutionary Paradox of Interactions among Immune Mechanisms and Autologous or Allogeneic Gametes and Semiallogeneic Foetuses. Veterinary Quarterly 2020, 40 (1), 353–383. https://doi.org/10.1080/01652176.2020.1852336.. in Veterinary Quarterly. 2020;41(1):152-152.
doi:10.1080/01652176.2020.1852336 .

Samardžija, Marko, Lojkić, Martina, Maćešić, Nino, Valpotić, Hrvoje, Butković, Ivan, Šavorić, Juraj, Žura Žaja, Ivona, Leiner, Denis, Đuričić, Dražen, Marković, Franjo, Kočila, Predrag, Vidas, Željko, Gerenčer, Marijan, Kaštelan, Andrija, Milovanović, Aleksandar, Lazarević, Miodrag, Rukavina, Daniel, Valpotić, Ivica, "Erratum: Samardžija, M.; Lojkić, M.; Maćešić, N.; Valpotić, H.; Butković, I.; Šavorić, J.; Žura Žaja, I.; Leiner, D.; Đuričić, D.; Marković, F.; Kočila, P.; Vidas, Ž.; Gerenčer, M.; Kaštelan, A.; Milovanović, A.; Lazarević, M.; Rukavina, D.; Valpotić, I. Reproductive Immunology in Viviparous Mammals: Evolutionary Paradox of Interactions among Immune Mechanisms and Autologous or Allogeneic Gametes and Semiallogeneic Foetuses. Veterinary Quarterly 2020, 40 (1), 353–383. https://doi.org/10.1080/01652176.2020.1852336." in Veterinary Quarterly, 41, no. 1 (2020):152-152,
https://doi.org/10.1080/01652176.2020.1852336 . .

TY  - JOUR
AU  - Stojanov, Igor
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Prodanov-Radulović, Jasna
AU  - Apić, Jelena
AU  - Stojanović, Dragica
AU  - Maksimović, Nevena
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2385
AB  - The aim of the study was to determine whether the bacteria from the environment and from the mucous membrane of the boar prepuce have antimicrobial resistance and whether the result obtained is similar/same to the bacteria that can be found in native boar semen. The study addresses the problem of the presence of primarily resistant bacterial strains in the boar sperm, which, due to their reduced sensitivity, cannot be suppressed by antibiotics used in the semen dilution agent, as well as to emphasize the importance of microbiological monitoring of the boar mucous membranes and ambient surfaces before and during their exploitation. Such an examination could contribute to the interchangeable design of the dilution agent for the boar semen relative to the antibiotic content.Resistant strains of bacteria from prepuce swabs and swabs taken from the facility, as well as from native boar semen were isolated. The presence of these bacteria affected the quality of the semen. In conclusion, it should be pointed out that bacterial monitoring of the prepuce and surface of the facility can indicate possible problems related to the quality of semen, and that the design of the dilution agent for boar semen should be adjusted to the established resistance of isolated bacteria.
AB  - Cilj ispitivanja je da se utvrdi da li bakterije iz okoline i sa sluznici prepucijma poseduju antimikrobnu rezistenciju i da li je dobijeni nalaz sličan/isti sa bakterijama koje se mogu naći u nativnom semenu nerasta. Ispitivanje treba da odgovori na problem prisustva, pre svega rezistentnih bakterijskih sojeva u spermi nerasta koji zbog svoje smanjene osteljivosti ne mogu biti suprimirani antibioticima, koji se nalaze u razređivaču za seme, kao i da istakne značaj mikrobiološkog monitoringa sluznice nerasta i ambijentalnih površina pre i tokom njihove eksploatacije. Ovakvo ispitivanje bi moglo da doprinese izmenjivom dizajniranju razređivača za seme nerasta u odnosu na sadržaj antibiotika. Izolovani su rezistentni sojevi bakterija iz briseva prepucijuma i briseva uzetih iz objekta kao i iz nativnog semena nerasta. Prisustvo ovih bakterija uticalo na kvalitet semena. Kao zaključak treba istaći da bakterijski monitoring prepucijuma i površina u objektu može ukazati na moguće probleme vezane za kvalitet semena nerasta kao i da se dizajniranje razređivača za seme nerasta prilagodi utvrđenoj rezistenciji izolovanih bakterija.
PB  - Faculty of Veterinary Medicine
PB  - Sciendo
T2  - Acta Veterinaria-Beograd
T1  - Antimicrobial resistance as a problem for the quality of Boar Semen
T1  - Antimikrobna rezistencija kao problem kvaliteta semena nerasta
VL  - 70
IS  - 1
SP  - 136
EP  - 146
DO  - 10.2478/acve-2020-0010
ER  - 

@article{
author = "Stojanov, Igor and Milovanović, Aleksandar and Barna, Tomislav and Prodanov-Radulović, Jasna and Apić, Jelena and Stojanović, Dragica and Maksimović, Nevena",
year = "2020",
abstract = "The aim of the study was to determine whether the bacteria from the environment and from the mucous membrane of the boar prepuce have antimicrobial resistance and whether the result obtained is similar/same to the bacteria that can be found in native boar semen. The study addresses the problem of the presence of primarily resistant bacterial strains in the boar sperm, which, due to their reduced sensitivity, cannot be suppressed by antibiotics used in the semen dilution agent, as well as to emphasize the importance of microbiological monitoring of the boar mucous membranes and ambient surfaces before and during their exploitation. Such an examination could contribute to the interchangeable design of the dilution agent for the boar semen relative to the antibiotic content.Resistant strains of bacteria from prepuce swabs and swabs taken from the facility, as well as from native boar semen were isolated. The presence of these bacteria affected the quality of the semen. In conclusion, it should be pointed out that bacterial monitoring of the prepuce and surface of the facility can indicate possible problems related to the quality of semen, and that the design of the dilution agent for boar semen should be adjusted to the established resistance of isolated bacteria., Cilj ispitivanja je da se utvrdi da li bakterije iz okoline i sa sluznici prepucijma poseduju antimikrobnu rezistenciju i da li je dobijeni nalaz sličan/isti sa bakterijama koje se mogu naći u nativnom semenu nerasta. Ispitivanje treba da odgovori na problem prisustva, pre svega rezistentnih bakterijskih sojeva u spermi nerasta koji zbog svoje smanjene osteljivosti ne mogu biti suprimirani antibioticima, koji se nalaze u razređivaču za seme, kao i da istakne značaj mikrobiološkog monitoringa sluznice nerasta i ambijentalnih površina pre i tokom njihove eksploatacije. Ovakvo ispitivanje bi moglo da doprinese izmenjivom dizajniranju razređivača za seme nerasta u odnosu na sadržaj antibiotika. Izolovani su rezistentni sojevi bakterija iz briseva prepucijuma i briseva uzetih iz objekta kao i iz nativnog semena nerasta. Prisustvo ovih bakterija uticalo na kvalitet semena. Kao zaključak treba istaći da bakterijski monitoring prepucijuma i površina u objektu može ukazati na moguće probleme vezane za kvalitet semena nerasta kao i da se dizajniranje razređivača za seme nerasta prilagodi utvrđenoj rezistenciji izolovanih bakterija.",
publisher = "Faculty of Veterinary Medicine, Sciendo",
journal = "Acta Veterinaria-Beograd",
title = "Antimicrobial resistance as a problem for the quality of Boar Semen, Antimikrobna rezistencija kao problem kvaliteta semena nerasta",
volume = "70",
number = "1",
pages = "136-146",
doi = "10.2478/acve-2020-0010"
}

Stojanov, I., Milovanović, A., Barna, T., Prodanov-Radulović, J., Apić, J., Stojanović, D.,& Maksimović, N.. (2020). Antimicrobial resistance as a problem for the quality of Boar Semen. in Acta Veterinaria-Beograd
Faculty of Veterinary Medicine., 70(1), 136-146.
https://doi.org/10.2478/acve-2020-0010

Stojanov I, Milovanović A, Barna T, Prodanov-Radulović J, Apić J, Stojanović D, Maksimović N. Antimicrobial resistance as a problem for the quality of Boar Semen. in Acta Veterinaria-Beograd. 2020;70(1):136-146.
doi:10.2478/acve-2020-0010 .

Stojanov, Igor, Milovanović, Aleksandar, Barna, Tomislav, Prodanov-Radulović, Jasna, Apić, Jelena, Stojanović, Dragica, Maksimović, Nevena, "Antimicrobial resistance as a problem for the quality of Boar Semen" in Acta Veterinaria-Beograd, 70, no. 1 (2020):136-146,
https://doi.org/10.2478/acve-2020-0010 . .

TY  - JOUR
AU  - Zekic-Stosić, Marina
AU  - Kanacki, Zdenko
AU  - Stojanović, Dragica
AU  - Bugarski, Dejan
AU  - Lazarević, Miodrag
AU  - Milovanović, Aleksandar
AU  - Masić, Aleksandar
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1593
AB  - Introduction: Hormonal and metabolic changes, as well as energy imbalance, can affect health, production and reproductive performance of dairy cows. In the present study, we evaluated phagocytosis and respiratory burst neutrophil activity during the transition period and early lactation and compared it with biochemical and hematological parameters in dairy cows. Methodology: Simmental cows (n = 21) were enrolled in the study. Whole blood samples were collected weekly from 3 weeks pre- calving until 6 weeks post calving. Basic metabolic and blood parameters were assessed by routine laboratory analyses, while neutrophil functions were analyzed by commercial test kits. Results: Optimal neutrophil response was observed pre and post calving. The highest value was recorded in the 6th week after calving (89.54 +/- 7.61%) and being significantly higher (p < 0.01) as compared to values recorded at two and one week before and one week after calving. The percentage of activated neutrophils was high during the entire study period: from 70.80 +/- 5.22% at the beginning of the study to 89.54 +/- 7.61% at the end of the study. During the study period, production of Reactive Oxidative Species by neutrophils was positively correlated with beta-hydroxybutyrat and non-esteri tied fatty acids values (0.454** and 0.423**, respectively) and calcium levels (0.164* and 0.212**, respectively). Conclusions: The most prominent changes in all parameters had no influence on phagocytic and respiratory burst activity of neutrophils. Neutrophil function is preserved at the optimal level during the transition period and early lactation in Simmental cows.
PB  - J Infection Developing Countries, Tramaniglio
T2  - Journal of Infection in Developing Countries
T1  - Neutrophil phagocytosis and respiratory burst activity of dairy cows during the transition period and early lactation
VL  - 12
IS  - 10
SP  - 894
DO  - 10.3855/jidc.10767
ER  - 

@article{
author = "Zekic-Stosić, Marina and Kanacki, Zdenko and Stojanović, Dragica and Bugarski, Dejan and Lazarević, Miodrag and Milovanović, Aleksandar and Masić, Aleksandar",
year = "2018",
abstract = "Introduction: Hormonal and metabolic changes, as well as energy imbalance, can affect health, production and reproductive performance of dairy cows. In the present study, we evaluated phagocytosis and respiratory burst neutrophil activity during the transition period and early lactation and compared it with biochemical and hematological parameters in dairy cows. Methodology: Simmental cows (n = 21) were enrolled in the study. Whole blood samples were collected weekly from 3 weeks pre- calving until 6 weeks post calving. Basic metabolic and blood parameters were assessed by routine laboratory analyses, while neutrophil functions were analyzed by commercial test kits. Results: Optimal neutrophil response was observed pre and post calving. The highest value was recorded in the 6th week after calving (89.54 +/- 7.61%) and being significantly higher (p < 0.01) as compared to values recorded at two and one week before and one week after calving. The percentage of activated neutrophils was high during the entire study period: from 70.80 +/- 5.22% at the beginning of the study to 89.54 +/- 7.61% at the end of the study. During the study period, production of Reactive Oxidative Species by neutrophils was positively correlated with beta-hydroxybutyrat and non-esteri tied fatty acids values (0.454** and 0.423**, respectively) and calcium levels (0.164* and 0.212**, respectively). Conclusions: The most prominent changes in all parameters had no influence on phagocytic and respiratory burst activity of neutrophils. Neutrophil function is preserved at the optimal level during the transition period and early lactation in Simmental cows.",
publisher = "J Infection Developing Countries, Tramaniglio",
journal = "Journal of Infection in Developing Countries",
title = "Neutrophil phagocytosis and respiratory burst activity of dairy cows during the transition period and early lactation",
volume = "12",
number = "10",
pages = "894",
doi = "10.3855/jidc.10767"
}

Zekic-Stosić, M., Kanacki, Z., Stojanović, D., Bugarski, D., Lazarević, M., Milovanović, A.,& Masić, A.. (2018). Neutrophil phagocytosis and respiratory burst activity of dairy cows during the transition period and early lactation. in Journal of Infection in Developing Countries
J Infection Developing Countries, Tramaniglio., 12(10), 894.
https://doi.org/10.3855/jidc.10767

Zekic-Stosić M, Kanacki Z, Stojanović D, Bugarski D, Lazarević M, Milovanović A, Masić A. Neutrophil phagocytosis and respiratory burst activity of dairy cows during the transition period and early lactation. in Journal of Infection in Developing Countries. 2018;12(10):894.
doi:10.3855/jidc.10767 .

Zekic-Stosić, Marina, Kanacki, Zdenko, Stojanović, Dragica, Bugarski, Dejan, Lazarević, Miodrag, Milovanović, Aleksandar, Masić, Aleksandar, "Neutrophil phagocytosis and respiratory burst activity of dairy cows during the transition period and early lactation" in Journal of Infection in Developing Countries, 12, no. 10 (2018):894,
https://doi.org/10.3855/jidc.10767 . .

TY  - JOUR
AU  - Romanowski, Radoslaw
AU  - Culbert, Rick
AU  - Alkemade, Stan
AU  - Medellin-Pena, Maira J.
AU  - Bugarski, Dejan
AU  - Milovanović, Aleksandar
AU  - Nešić, Slađan
AU  - Masić, Aleksandar
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1479
AB  - Calf diarrhea is a common disease in young animals and the primary cause of productivity and economic losses to cattle producers worldwide. According to the report from the National Animal Health Monitoring System for U.S. dairy, more than fifty percent of the deaths of un-weaned calves is attributed to severe diarrhea. Enterotoxigenic Escherichia coli (ETEC) strain K99+ remains the most common pathogen isolated from calves which are younger than three days of age. Dam vaccination and the use of antimicrobials remain the most used prophylactic and treatment options for calf diarrhea. The rise in antibiotic resistance around the world has been a major concern and new alternative therapies have been explored. Mycobacterium Cell Wall Fraction (MCWF) is a biological immunomodulator that has a potential in multiple veterinary health services such as the treatment and prevention of infectious diseases and anticancer therapy in both small and large animals. The efficacy of the MCWF in reducing the severity of neonatal calf diarrhea and its associated mortality, following challenge with ETEC K99+ was evaluated. Twenty-three calves were placed into two experimental groups. Eleven calves received a single 1 mL dose of MCWF intravenously (IV) at the onset of clinical signs of disease following challenge. Twelve non-treated, challenged calves were retained as controls. The severity and duration of diarrhea was significantly reduced in the MCWF treated group compared to untreated controls. In addition, the mortality rate in the MCWF treated group was significantly reduced to 10% while the observed mortality in the control group reached 58%. Data from the current study suggest that MCWF could be used as an alternative treatment to reduce the severity, duration and mortality of ETEC induced diarrhea in neonatal calves.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Mycobacterium cell wall fraction immunostimulant (amplimune (tm)) efficacy in the reduction of the severity of etec induced diarrhea in neonatal calves
VL  - 67
IS  - 2
SP  - 228
EP  - 243
DO  - 10.1515/acve-2017-0019
ER  - 

@article{
author = "Romanowski, Radoslaw and Culbert, Rick and Alkemade, Stan and Medellin-Pena, Maira J. and Bugarski, Dejan and Milovanović, Aleksandar and Nešić, Slađan and Masić, Aleksandar",
year = "2017",
abstract = "Calf diarrhea is a common disease in young animals and the primary cause of productivity and economic losses to cattle producers worldwide. According to the report from the National Animal Health Monitoring System for U.S. dairy, more than fifty percent of the deaths of un-weaned calves is attributed to severe diarrhea. Enterotoxigenic Escherichia coli (ETEC) strain K99+ remains the most common pathogen isolated from calves which are younger than three days of age. Dam vaccination and the use of antimicrobials remain the most used prophylactic and treatment options for calf diarrhea. The rise in antibiotic resistance around the world has been a major concern and new alternative therapies have been explored. Mycobacterium Cell Wall Fraction (MCWF) is a biological immunomodulator that has a potential in multiple veterinary health services such as the treatment and prevention of infectious diseases and anticancer therapy in both small and large animals. The efficacy of the MCWF in reducing the severity of neonatal calf diarrhea and its associated mortality, following challenge with ETEC K99+ was evaluated. Twenty-three calves were placed into two experimental groups. Eleven calves received a single 1 mL dose of MCWF intravenously (IV) at the onset of clinical signs of disease following challenge. Twelve non-treated, challenged calves were retained as controls. The severity and duration of diarrhea was significantly reduced in the MCWF treated group compared to untreated controls. In addition, the mortality rate in the MCWF treated group was significantly reduced to 10% while the observed mortality in the control group reached 58%. Data from the current study suggest that MCWF could be used as an alternative treatment to reduce the severity, duration and mortality of ETEC induced diarrhea in neonatal calves.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Mycobacterium cell wall fraction immunostimulant (amplimune (tm)) efficacy in the reduction of the severity of etec induced diarrhea in neonatal calves",
volume = "67",
number = "2",
pages = "228-243",
doi = "10.1515/acve-2017-0019"
}

Romanowski, R., Culbert, R., Alkemade, S., Medellin-Pena, M. J., Bugarski, D., Milovanović, A., Nešić, S.,& Masić, A.. (2017). Mycobacterium cell wall fraction immunostimulant (amplimune (tm)) efficacy in the reduction of the severity of etec induced diarrhea in neonatal calves. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(2), 228-243.
https://doi.org/10.1515/acve-2017-0019

Romanowski R, Culbert R, Alkemade S, Medellin-Pena MJ, Bugarski D, Milovanović A, Nešić S, Masić A. Mycobacterium cell wall fraction immunostimulant (amplimune (tm)) efficacy in the reduction of the severity of etec induced diarrhea in neonatal calves. in Acta Veterinaria-Beograd. 2017;67(2):228-243.
doi:10.1515/acve-2017-0019 .

Romanowski, Radoslaw, Culbert, Rick, Alkemade, Stan, Medellin-Pena, Maira J., Bugarski, Dejan, Milovanović, Aleksandar, Nešić, Slađan, Masić, Aleksandar, "Mycobacterium cell wall fraction immunostimulant (amplimune (tm)) efficacy in the reduction of the severity of etec induced diarrhea in neonatal calves" in Acta Veterinaria-Beograd, 67, no. 2 (2017):228-243,
https://doi.org/10.1515/acve-2017-0019 . .

TY  - JOUR
AU  - Lazarević, Miodrag
AU  - Milovanović, Aleksandar
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2152
AB  - U ovom revijalnom radu su izneta savremena shvatanja mehanizama delovanja,
uloga i mogućnosti primene imunostimulatornih supstanci u veterinarskoj medicini.
Imunofarmakologija je ne tako nova grana farmakologije, nastala iz sve očiglednije
potrebe da se lekovima i drugim prepratima utiče na funkcije imunskog sistema, kako
ljudi tako i životinja. U veterinarskoj medicini inunostimulatori imaju poseban značaj
zbog toga što postoji stalna potreba za zaštitom zdravlja životinja u uslovima intenzivne
proizvodnje. Jedan od najvećih problema u primeni imunomodulatornih supstanci
leži u činjenici da jedna te ista supstancija, u zavisnosti od načina i režima doziranja,
može delovati imunosupresivno ili imunostimulatorno. Ovi efekti zavise i od trenutnog
funkcionalnog stanja imunskog sistema samog pacijenta. Osim prikaza literaturnih
podataka, izneta su i sopstvena iskustva autora proizašla iz brojnih laboratorijskih
ispitivanja i ogleda na farmama. Ona se pre svega odnose na primenu manan-oligosaharida
u povećanju stepena resorpcije kolostralnih imunoglobulina kod teladi i prasadi,
primenu manan-oligosaharida u terapiji puerperalne disgalakcije krmača, primenu
ekstrakta goveđeg timusa kod pacova sa imunosupresijom izazvanom jonizujućim
zracima, primenu ekstrakta zida Mycobacterium phlei (Mcb. phlei) u poboljšanju
reproduktivnih rezultata krmača, primenu ekstrakta ostriga u terapiji endometritisa
krava i primenu visokih doza vitamina C u terapiji subkliničkih oblika mastitisa mlečnih
krava. Takođe je opisana i imunomodulatorna uloga antihelmintika levamizola ispitana
na modelu eksperimentalnog autoimunog encefalomijelitisa pacova kao i uticaj
ekstrakta kukureka (Helleborus odorus Waldst et Kit) na nespecifične odbrambene
mehanizme pacova.
AB  - This review article deals with the contemporary understanding of the action mechanisms,
roles and possibilities for immunostimulatory substances usage in the veterinary
medicine. Immunopharmacology is not that new branch of pharamacology,
raised from the obvious need to influence functions of the immune system both in
humans and animals by medicaments or other substances. In veterinary medicine
inunostimulants are of special significance because there is a permanent need for animal’s
health protection under conditions of intensive production. Immunostimulants
achieved a special significance since the total ban of antibiotics usage as growth stimulators
resulting in reduced health protection, especially in the intensive production.
One of the major problems regarding immunomodulators lays in the fact that the
same drug, depending on the regime and dose, may exert both immunosuppressive
and immunostimulatory effects. These effects are also dependent on the current status
of the immune system functions of the particular patient. In adition to the literature
data, we present our own experiences raised from the numerous laboratory
investigations and field trials. This accomplish usage of mannan-oligosacharides in
enhancing degree of absorption of colostral immunoglobulins in calves and piglets,
application of the bovine thymic extract in irradiated immunosuppressed rats, usage
of the mannan-oligosacharides in the therapy of puerperal dysgalactia in sows, usage
of Mcb. phlei call wall extract in improving reproductive results in sows, usage of the
oyster extract in the therapy of cows endometritis and application of the high vitamin
C doses in the therapy of subclinical mastites in dairy cows. Immunomodulatory effects
of anthelmintic drug, Levamisole, in the model of Experimental Autoimmune Encephalomyelitis
of rats are described as well. In addition, we have investigated effects
of Helleborus odorus (Waldst et Kit) extracts on innate defense mechanisms in rats.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Primena pojedinih imunostimulatora u veterinarskoj medicini
T1  - Application of some immunostimulators in veterinary medicine
VL  - 17
IS  - 1
SP  - 16
EP  - 36
DO  - 10.7251/VETJ1701016L
ER  - 

@article{
author = "Lazarević, Miodrag and Milovanović, Aleksandar",
year = "2017",
abstract = "U ovom revijalnom radu su izneta savremena shvatanja mehanizama delovanja,
uloga i mogućnosti primene imunostimulatornih supstanci u veterinarskoj medicini.
Imunofarmakologija je ne tako nova grana farmakologije, nastala iz sve očiglednije
potrebe da se lekovima i drugim prepratima utiče na funkcije imunskog sistema, kako
ljudi tako i životinja. U veterinarskoj medicini inunostimulatori imaju poseban značaj
zbog toga što postoji stalna potreba za zaštitom zdravlja životinja u uslovima intenzivne
proizvodnje. Jedan od najvećih problema u primeni imunomodulatornih supstanci
leži u činjenici da jedna te ista supstancija, u zavisnosti od načina i režima doziranja,
može delovati imunosupresivno ili imunostimulatorno. Ovi efekti zavise i od trenutnog
funkcionalnog stanja imunskog sistema samog pacijenta. Osim prikaza literaturnih
podataka, izneta su i sopstvena iskustva autora proizašla iz brojnih laboratorijskih
ispitivanja i ogleda na farmama. Ona se pre svega odnose na primenu manan-oligosaharida
u povećanju stepena resorpcije kolostralnih imunoglobulina kod teladi i prasadi,
primenu manan-oligosaharida u terapiji puerperalne disgalakcije krmača, primenu
ekstrakta goveđeg timusa kod pacova sa imunosupresijom izazvanom jonizujućim
zracima, primenu ekstrakta zida Mycobacterium phlei (Mcb. phlei) u poboljšanju
reproduktivnih rezultata krmača, primenu ekstrakta ostriga u terapiji endometritisa
krava i primenu visokih doza vitamina C u terapiji subkliničkih oblika mastitisa mlečnih
krava. Takođe je opisana i imunomodulatorna uloga antihelmintika levamizola ispitana
na modelu eksperimentalnog autoimunog encefalomijelitisa pacova kao i uticaj
ekstrakta kukureka (Helleborus odorus Waldst et Kit) na nespecifične odbrambene
mehanizme pacova., This review article deals with the contemporary understanding of the action mechanisms,
roles and possibilities for immunostimulatory substances usage in the veterinary
medicine. Immunopharmacology is not that new branch of pharamacology,
raised from the obvious need to influence functions of the immune system both in
humans and animals by medicaments or other substances. In veterinary medicine
inunostimulants are of special significance because there is a permanent need for animal’s
health protection under conditions of intensive production. Immunostimulants
achieved a special significance since the total ban of antibiotics usage as growth stimulators
resulting in reduced health protection, especially in the intensive production.
One of the major problems regarding immunomodulators lays in the fact that the
same drug, depending on the regime and dose, may exert both immunosuppressive
and immunostimulatory effects. These effects are also dependent on the current status
of the immune system functions of the particular patient. In adition to the literature
data, we present our own experiences raised from the numerous laboratory
investigations and field trials. This accomplish usage of mannan-oligosacharides in
enhancing degree of absorption of colostral immunoglobulins in calves and piglets,
application of the bovine thymic extract in irradiated immunosuppressed rats, usage
of the mannan-oligosacharides in the therapy of puerperal dysgalactia in sows, usage
of Mcb. phlei call wall extract in improving reproductive results in sows, usage of the
oyster extract in the therapy of cows endometritis and application of the high vitamin
C doses in the therapy of subclinical mastites in dairy cows. Immunomodulatory effects
of anthelmintic drug, Levamisole, in the model of Experimental Autoimmune Encephalomyelitis
of rats are described as well. In addition, we have investigated effects
of Helleborus odorus (Waldst et Kit) extracts on innate defense mechanisms in rats.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Primena pojedinih imunostimulatora u veterinarskoj medicini, Application of some immunostimulators in veterinary medicine",
volume = "17",
number = "1",
pages = "16-36",
doi = "10.7251/VETJ1701016L"
}

Lazarević, M.,& Milovanović, A.. (2017). Primena pojedinih imunostimulatora u veterinarskoj medicini. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 17(1), 16-36.
https://doi.org/10.7251/VETJ1701016L

Lazarević M, Milovanović A. Primena pojedinih imunostimulatora u veterinarskoj medicini. in Veterinarski žurnal Republike Srpske. 2017;17(1):16-36.
doi:10.7251/VETJ1701016L .

Lazarević, Miodrag, Milovanović, Aleksandar, "Primena pojedinih imunostimulatora u veterinarskoj medicini" in Veterinarski žurnal Republike Srpske, 17, no. 1 (2017):16-36,
https://doi.org/10.7251/VETJ1701016L . .

TY  - JOUR
AU  - Apić, Jelena
AU  - Vakanjac, Slobodanka
AU  - Radović, Ivan
AU  - Jotanović, Stoja
AU  - Živkov-Baloš, Milica
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Stanković, Branislav
AU  - Jajić, Igor
AU  - Maletić, Milan
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1276
AB  - Recently, it was frequently demonstrated that fertility of sows after artificially inseminated is lower than after mating. This is associated with a reduced fertilization capacity of overdiluted insemination doses. The aim of this study was to investigate the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17 degrees C for 3 days. Progressive motility was significantly higher (p<0.01) in the ejaculates with high, compared to the ejaculates with low protein content (82% vs. 76%). After 3 days of storage, in the1:4 dilution proportion, the average progressive motility was significantly (p<0.01) decreased in relation to this value in native semen from the boars with high (82% to 64%), as well from the boars with low protein content in seminal plasma (76% to 48%). However, the average diluted semen progressive motility was significantly greater (p<0.01) in the boars with high (64%), compared to the boars with low protein content in seminal plasma (48%). The number of good diluted semen samples (>= 65% progressive motility), was also significantly (p<0.01) greater in the boars with high (41%), compared to the boars with low protein content in seminal plasma (12%). These results show that seminal plasma proteins play an important role in maintaining the sperm progressive motility of diluted semen in vitro stored for 3 days.
PB  - Slovak Univ Agriculture Nitra, Nitra
T2  - Journal of Microbiology Biotechnology and Food Sciences
T1  - Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days
VL  - 4
SP  - 1
EP  - 3
DO  - 10.15414/jmbfs.2015.4.special2.1-3
ER  - 

@article{
author = "Apić, Jelena and Vakanjac, Slobodanka and Radović, Ivan and Jotanović, Stoja and Živkov-Baloš, Milica and Milovanović, Aleksandar and Barna, Tomislav and Stanković, Branislav and Jajić, Igor and Maletić, Milan",
year = "2015",
abstract = "Recently, it was frequently demonstrated that fertility of sows after artificially inseminated is lower than after mating. This is associated with a reduced fertilization capacity of overdiluted insemination doses. The aim of this study was to investigate the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17 degrees C for 3 days. Progressive motility was significantly higher (p<0.01) in the ejaculates with high, compared to the ejaculates with low protein content (82% vs. 76%). After 3 days of storage, in the1:4 dilution proportion, the average progressive motility was significantly (p<0.01) decreased in relation to this value in native semen from the boars with high (82% to 64%), as well from the boars with low protein content in seminal plasma (76% to 48%). However, the average diluted semen progressive motility was significantly greater (p<0.01) in the boars with high (64%), compared to the boars with low protein content in seminal plasma (48%). The number of good diluted semen samples (>= 65% progressive motility), was also significantly (p<0.01) greater in the boars with high (41%), compared to the boars with low protein content in seminal plasma (12%). These results show that seminal plasma proteins play an important role in maintaining the sperm progressive motility of diluted semen in vitro stored for 3 days.",
publisher = "Slovak Univ Agriculture Nitra, Nitra",
journal = "Journal of Microbiology Biotechnology and Food Sciences",
title = "Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days",
volume = "4",
pages = "1-3",
doi = "10.15414/jmbfs.2015.4.special2.1-3"
}

Apić, J., Vakanjac, S., Radović, I., Jotanović, S., Živkov-Baloš, M., Milovanović, A., Barna, T., Stanković, B., Jajić, I.,& Maletić, M.. (2015). Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days. in Journal of Microbiology Biotechnology and Food Sciences
Slovak Univ Agriculture Nitra, Nitra., 4, 1-3.
https://doi.org/10.15414/jmbfs.2015.4.special2.1-3

Apić J, Vakanjac S, Radović I, Jotanović S, Živkov-Baloš M, Milovanović A, Barna T, Stanković B, Jajić I, Maletić M. Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days. in Journal of Microbiology Biotechnology and Food Sciences. 2015;4:1-3.
doi:10.15414/jmbfs.2015.4.special2.1-3 .

Apić, Jelena, Vakanjac, Slobodanka, Radović, Ivan, Jotanović, Stoja, Živkov-Baloš, Milica, Milovanović, Aleksandar, Barna, Tomislav, Stanković, Branislav, Jajić, Igor, Maletić, Milan, "Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days" in Journal of Microbiology Biotechnology and Food Sciences, 4 (2015):1-3,
https://doi.org/10.15414/jmbfs.2015.4.special2.1-3 . .

TY  - JOUR
AU  - Milovanović, Aleksandar
AU  - Maksimović, N.
AU  - Barna, Tomislav
AU  - Lazarević, Miodrag
AU  - Delić, N.
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1025
AB  - This paper describes a procedure of laparoscopic insemination (LAI) performed in 42 Ile de France ewes with deep-frozen rams' semen. Frozen semen doses was imported from France, originating from two rams (A and B), and used intermittently in three LAI cycles on different females. The ewes were examined ultrasonographically for pregnancy, two months following insemination and data of lambing were recorded at the end. Total achieved lambing rate was 61.90% (26/42) and total number of lambs was 49 (prolificacy 1.88; 1.16 lambs per sheep included in LAI protocol). Three labs from 2 ewes were stillborn and one dyed at 10th day after lambing. Lambing rates for rams A and B were 53.85% (14/26) and 75.00% (12/16), respectively. Introduction of laparoscopic insemination of sheep with imported deep-frozen semen of top quality rams, gaining technical experience and achieving conception rate over 65% could make this technique acceptable in improving genetic potential of top quality sheep flocks.
AB  - U radu je opisan postupak laparoskopskog osemenjavanja (LAO) primenjen kod 42 ovce rase Ile de France duboko zamrznutim semenom. Tokom tri ciklusa osemenjavanja naizmenično je korišćeno seme dva ovna (A i B), uvezeno iz Francuske. Nakon dva meseca od osemenjavanja ovce su pregledane na sjagnjenost ultrasonografskom metodom i analizirani su podaci o jagnjenju. Ostvarena je koncepcija od 61,90% (26/42) i dobijeno je ukupno 49 jaganjaca (indeks jagnjena 1,88 po ojagnjenoj ovci, odnosno, 1,16 jagnjeta po ovci uključenoj u protokol LAO). Tri jagnjeta od dve ovce bila su mrtvorođena i jedno jagnje je uginulo 10. dana nakon jagnjenja. Sjagnjenost od semena ovna A iznosila je 58,85% (14/26) a od ovna B 75,00% (12/16). Uvođenje laparoskopskog osemenjavanja ovaca, uvoz duboko-zamrznutog semena vrhunskih ovnova, sticanje iskustva i ostvarenje koncepcije od preko 65% može ovu tehniku učiniti prihvatljivom u popravljanju genetskog potencijala kvalitetnih stada ovaca.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Laparoscopic insemination of sheep in Republic of Serbia
T1  - Laparoskopsko osemenjavanje ovaca u Republici Srbiji
VL  - 29
IS  - 3
SP  - 449
EP  - 456
DO  - 10.2298/BAH1303449M
ER  - 

@article{
author = "Milovanović, Aleksandar and Maksimović, N. and Barna, Tomislav and Lazarević, Miodrag and Delić, N.",
year = "2013",
abstract = "This paper describes a procedure of laparoscopic insemination (LAI) performed in 42 Ile de France ewes with deep-frozen rams' semen. Frozen semen doses was imported from France, originating from two rams (A and B), and used intermittently in three LAI cycles on different females. The ewes were examined ultrasonographically for pregnancy, two months following insemination and data of lambing were recorded at the end. Total achieved lambing rate was 61.90% (26/42) and total number of lambs was 49 (prolificacy 1.88; 1.16 lambs per sheep included in LAI protocol). Three labs from 2 ewes were stillborn and one dyed at 10th day after lambing. Lambing rates for rams A and B were 53.85% (14/26) and 75.00% (12/16), respectively. Introduction of laparoscopic insemination of sheep with imported deep-frozen semen of top quality rams, gaining technical experience and achieving conception rate over 65% could make this technique acceptable in improving genetic potential of top quality sheep flocks., U radu je opisan postupak laparoskopskog osemenjavanja (LAO) primenjen kod 42 ovce rase Ile de France duboko zamrznutim semenom. Tokom tri ciklusa osemenjavanja naizmenično je korišćeno seme dva ovna (A i B), uvezeno iz Francuske. Nakon dva meseca od osemenjavanja ovce su pregledane na sjagnjenost ultrasonografskom metodom i analizirani su podaci o jagnjenju. Ostvarena je koncepcija od 61,90% (26/42) i dobijeno je ukupno 49 jaganjaca (indeks jagnjena 1,88 po ojagnjenoj ovci, odnosno, 1,16 jagnjeta po ovci uključenoj u protokol LAO). Tri jagnjeta od dve ovce bila su mrtvorođena i jedno jagnje je uginulo 10. dana nakon jagnjenja. Sjagnjenost od semena ovna A iznosila je 58,85% (14/26) a od ovna B 75,00% (12/16). Uvođenje laparoskopskog osemenjavanja ovaca, uvoz duboko-zamrznutog semena vrhunskih ovnova, sticanje iskustva i ostvarenje koncepcije od preko 65% može ovu tehniku učiniti prihvatljivom u popravljanju genetskog potencijala kvalitetnih stada ovaca.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Laparoscopic insemination of sheep in Republic of Serbia, Laparoskopsko osemenjavanje ovaca u Republici Srbiji",
volume = "29",
number = "3",
pages = "449-456",
doi = "10.2298/BAH1303449M"
}

Milovanović, A., Maksimović, N., Barna, T., Lazarević, M.,& Delić, N.. (2013). Laparoscopic insemination of sheep in Republic of Serbia. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 29(3), 449-456.
https://doi.org/10.2298/BAH1303449M

Milovanović A, Maksimović N, Barna T, Lazarević M, Delić N. Laparoscopic insemination of sheep in Republic of Serbia. in Biotechnology in Animal Husbandry. 2013;29(3):449-456.
doi:10.2298/BAH1303449M .

Milovanović, Aleksandar, Maksimović, N., Barna, Tomislav, Lazarević, Miodrag, Delić, N., "Laparoscopic insemination of sheep in Republic of Serbia" in Biotechnology in Animal Husbandry, 29, no. 3 (2013):449-456,
https://doi.org/10.2298/BAH1303449M . .

TY  - JOUR
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Milanov, Dubravka
AU  - Lazarević, Miodrag
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1072
AB  - In this article we presented procedures and results of boar semen quality control performed at the Scientific Veterinary Institute 'Novi Sad' based on continuous cooperation with the farms' centers for boar semen production. The data obtained by computer analysis (CASA-computer assisted sperm analysis), flow cytometry and cyto-morphologic examination were used for semen quality evaluation. The selected parameters were compared with the reproductive results in sows, such as: farrowing rate, number of piglets per litter, ratio of piglets born alive and stillborn piglets). Semen quality evaluation based on spermatozoa progressive motility, sperm concentration, morphological characteristics and chromatine structure damage were used to give recommendations for semen processing, dilution degree, prospective therapy of boars, or, at least, their culing. Analysis of semen was complemented with seasonal bacterial cultivation and controls in cases of sudden drop on semen quality. Separate files containing semen quality graphs and reproductive indicators for easier monitoring were created for every boar. Systematic semen analyses performed by the use of several modern methods, along with periodic bacteriological control, offer possibilities for reliable assessment of boars' semen quality.
AB  - U ovom radu su opisani postupak i rezultati kontrole kvaliteta semena nerastova u Naučnom institutu za veterinarstvo 'Novi Sad' na osnovu kontinuirane saradnje sa farmskim centrima za proizvodnju semena nerastova. U analizi kvaliteta semena je korišćena kompjuterska analiza (CASA-computer assysted sperm analysis), protočna citometrija i cito-morfološki pregled. Odabrani parametri kvaliteta semena su upoređivani sa reproduktivnim parametrima krmača kao što su oprasivost, broj prasadi po leglu, odnos živorođene i mrtvorođene prasadi. Ocena kvaliteta semena na osnovu progresivne pokretljivosti, koncentracije spermatozoida, morfoloških odlika i oštećenja hromatina je korišćena za davanje preporuka o načinu pripreme semena, određivanje stepena razređenja ili sprovođenje eventualne terapije nerastova, odnosno, njihovog isključenja iz priploda. Ove analize semena se dopunjavaju sezonskim bakteriološkim pregledom i kontrolom u slučaju pada kvaliteta semena. Za svakog nerasta je otvorena kartoteka kvaliteta semena sa grafičkim prikazom i reproduktivnim pokazateljima, radi lakšeg praćenja. Kontinuirana sistematska analiza kvaliteta semena, kombinovana sa više savremenih metoda, dopunjena povremenim bakteriološkim pregledom, daje mogućnost pouzdane procene kvaliteta semena nerastova.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Model for cooperation between boar studs and laboratories for reproduction in boars' semen quality control
T1  - Model saradnje repro-centara i laboratorije za reprodukciju u kontroli kvaliteta semena nerastova
VL  - 6
IS  - 1
SP  - 57
EP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1072
ER  - 

@article{
author = "Milovanović, Aleksandar and Barna, Tomislav and Milanov, Dubravka and Lazarević, Miodrag",
year = "2013",
abstract = "In this article we presented procedures and results of boar semen quality control performed at the Scientific Veterinary Institute 'Novi Sad' based on continuous cooperation with the farms' centers for boar semen production. The data obtained by computer analysis (CASA-computer assisted sperm analysis), flow cytometry and cyto-morphologic examination were used for semen quality evaluation. The selected parameters were compared with the reproductive results in sows, such as: farrowing rate, number of piglets per litter, ratio of piglets born alive and stillborn piglets). Semen quality evaluation based on spermatozoa progressive motility, sperm concentration, morphological characteristics and chromatine structure damage were used to give recommendations for semen processing, dilution degree, prospective therapy of boars, or, at least, their culing. Analysis of semen was complemented with seasonal bacterial cultivation and controls in cases of sudden drop on semen quality. Separate files containing semen quality graphs and reproductive indicators for easier monitoring were created for every boar. Systematic semen analyses performed by the use of several modern methods, along with periodic bacteriological control, offer possibilities for reliable assessment of boars' semen quality., U ovom radu su opisani postupak i rezultati kontrole kvaliteta semena nerastova u Naučnom institutu za veterinarstvo 'Novi Sad' na osnovu kontinuirane saradnje sa farmskim centrima za proizvodnju semena nerastova. U analizi kvaliteta semena je korišćena kompjuterska analiza (CASA-computer assysted sperm analysis), protočna citometrija i cito-morfološki pregled. Odabrani parametri kvaliteta semena su upoređivani sa reproduktivnim parametrima krmača kao što su oprasivost, broj prasadi po leglu, odnos živorođene i mrtvorođene prasadi. Ocena kvaliteta semena na osnovu progresivne pokretljivosti, koncentracije spermatozoida, morfoloških odlika i oštećenja hromatina je korišćena za davanje preporuka o načinu pripreme semena, određivanje stepena razređenja ili sprovođenje eventualne terapije nerastova, odnosno, njihovog isključenja iz priploda. Ove analize semena se dopunjavaju sezonskim bakteriološkim pregledom i kontrolom u slučaju pada kvaliteta semena. Za svakog nerasta je otvorena kartoteka kvaliteta semena sa grafičkim prikazom i reproduktivnim pokazateljima, radi lakšeg praćenja. Kontinuirana sistematska analiza kvaliteta semena, kombinovana sa više savremenih metoda, dopunjena povremenim bakteriološkim pregledom, daje mogućnost pouzdane procene kvaliteta semena nerastova.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Model for cooperation between boar studs and laboratories for reproduction in boars' semen quality control, Model saradnje repro-centara i laboratorije za reprodukciju u kontroli kvaliteta semena nerastova",
volume = "6",
number = "1",
pages = "57-70",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1072"
}

Milovanović, A., Barna, T., Milanov, D.,& Lazarević, M.. (2013). Model for cooperation between boar studs and laboratories for reproduction in boars' semen quality control. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 6(1), 57-70.
https://hdl.handle.net/21.15107/rcub_veterinar_1072

Milovanović A, Barna T, Milanov D, Lazarević M. Model for cooperation between boar studs and laboratories for reproduction in boars' semen quality control. in Arhiv veterinarske medicine. 2013;6(1):57-70.
https://hdl.handle.net/21.15107/rcub_veterinar_1072 .

Milovanović, Aleksandar, Barna, Tomislav, Milanov, Dubravka, Lazarević, Miodrag, "Model for cooperation between boar studs and laboratories for reproduction in boars' semen quality control" in Arhiv veterinarske medicine, 6, no. 1 (2013):57-70,
https://hdl.handle.net/21.15107/rcub_veterinar_1072 .

TY  - JOUR
AU  - Lazarević, Miodrag
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Miljas, N.
AU  - Milanov, Dubravka
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/917
AB  - On the basis of our investigations it was possible to conclude that intrauterine treatment of sows with puerperal uterine infections with sterile YCW (Yeast Cell Wall) resulted in significant clinical improvement. The percent of recidivism was the lowest (10%) in groups of sows treated with 10 and 20 g of YCW. The degree of bacterial CFU (Colony Forming Units) reduction in samples of sows uterine flushings following instillation of YCW (5, 10 and 20 g) was wery high and ranged from 1361 to 1444 times, while in sows treated with Lotagen 2% solution (100 mL) this parametar was only 32. At the moment of weaning, piglets from sows treated with 10 and 20 g of YCW were heavier when compared to the control and Lotagen group and their DBWG (Daily Body Weight Gain) was higher when compared to the Lotagen and control group. Treatment of sows by IU instillation of YCW did not influence the number of piglets in the next breeding cycle.
AB  - Na osnovu rezultata naših istraživanja bilo je moguće zaključiti da tretman krmača obolelih od puerperalnih infekcija materice, sterilnim preparatom dobijenim iz zida kvasca (YCW), ima za posledicu značajan klinički napredak. Procenat krmača sa recidivom je bio najniži (10%) u grupi tretiranoj sa 10 i 20g YCW. Stepen redukcije broja bakterijskih kolonija (CFU) u uzorcima dobijenih iz materice krmača posle tretmana YCW preparatom je bio veoma visok i kretao se u opsegu od 1361 do 1444 puta. Kod obolelih krmača, tretiranih rastvorom Lotagena (2%), vrednost ovog parametra je bila svega 32. U momentu zalučenja, prasad krmača tretiranih YCW preparatom u količini od 10 i 20 g bila su teža u poređenju sa prasadima krmača grupe tretirane Lotagenom i prasadima kontrolnih (neteretiranih) krmača. Osim toga i njihovi prosečni dnevni prirasti su bili veći. Tretman krmača intrauterinom instilacijom preparata na bazi YCW nije negativno uticao na broj prasadi u sledećem reproduktivnom ciklusu.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Endometritis therapy in sows by intra uterine instillation of yeast cell wall solution
T1  - Terapija endometritisa krmača intrauterinom instilacijom preparata na bazi zida kvasca
VL  - 62
IS  - 5-6
SP  - 611
EP  - 626
DO  - 10.2298/AVB1206611L
ER  - 

@article{
author = "Lazarević, Miodrag and Milovanović, Aleksandar and Barna, Tomislav and Miljas, N. and Milanov, Dubravka",
year = "2012",
abstract = "On the basis of our investigations it was possible to conclude that intrauterine treatment of sows with puerperal uterine infections with sterile YCW (Yeast Cell Wall) resulted in significant clinical improvement. The percent of recidivism was the lowest (10%) in groups of sows treated with 10 and 20 g of YCW. The degree of bacterial CFU (Colony Forming Units) reduction in samples of sows uterine flushings following instillation of YCW (5, 10 and 20 g) was wery high and ranged from 1361 to 1444 times, while in sows treated with Lotagen 2% solution (100 mL) this parametar was only 32. At the moment of weaning, piglets from sows treated with 10 and 20 g of YCW were heavier when compared to the control and Lotagen group and their DBWG (Daily Body Weight Gain) was higher when compared to the Lotagen and control group. Treatment of sows by IU instillation of YCW did not influence the number of piglets in the next breeding cycle., Na osnovu rezultata naših istraživanja bilo je moguće zaključiti da tretman krmača obolelih od puerperalnih infekcija materice, sterilnim preparatom dobijenim iz zida kvasca (YCW), ima za posledicu značajan klinički napredak. Procenat krmača sa recidivom je bio najniži (10%) u grupi tretiranoj sa 10 i 20g YCW. Stepen redukcije broja bakterijskih kolonija (CFU) u uzorcima dobijenih iz materice krmača posle tretmana YCW preparatom je bio veoma visok i kretao se u opsegu od 1361 do 1444 puta. Kod obolelih krmača, tretiranih rastvorom Lotagena (2%), vrednost ovog parametra je bila svega 32. U momentu zalučenja, prasad krmača tretiranih YCW preparatom u količini od 10 i 20 g bila su teža u poređenju sa prasadima krmača grupe tretirane Lotagenom i prasadima kontrolnih (neteretiranih) krmača. Osim toga i njihovi prosečni dnevni prirasti su bili veći. Tretman krmača intrauterinom instilacijom preparata na bazi YCW nije negativno uticao na broj prasadi u sledećem reproduktivnom ciklusu.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Endometritis therapy in sows by intra uterine instillation of yeast cell wall solution, Terapija endometritisa krmača intrauterinom instilacijom preparata na bazi zida kvasca",
volume = "62",
number = "5-6",
pages = "611-626",
doi = "10.2298/AVB1206611L"
}

Lazarević, M., Milovanović, A., Barna, T., Miljas, N.,& Milanov, D.. (2012). Endometritis therapy in sows by intra uterine instillation of yeast cell wall solution. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(5-6), 611-626.
https://doi.org/10.2298/AVB1206611L

Lazarević M, Milovanović A, Barna T, Miljas N, Milanov D. Endometritis therapy in sows by intra uterine instillation of yeast cell wall solution. in Acta Veterinaria-Beograd. 2012;62(5-6):611-626.
doi:10.2298/AVB1206611L .

Lazarević, Miodrag, Milovanović, Aleksandar, Barna, Tomislav, Miljas, N., Milanov, Dubravka, "Endometritis therapy in sows by intra uterine instillation of yeast cell wall solution" in Acta Veterinaria-Beograd, 62, no. 5-6 (2012):611-626,
https://doi.org/10.2298/AVB1206611L . .

TY  - JOUR
AU  - Milovanović, Aleksandar
AU  - Milovanović, J.
AU  - Obrenović, Sonja
AU  - Milovanović, Anđela
AU  - Simonović, P.
AU  - Čemerikić, D.
AU  - Tačević, Z.
AU  - Petronić, Ivana
AU  - Grajić, M.
AU  - Kekuš, Divna
AU  - Popević, M.
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/830
AB  - Lyme borreliosis (LB) is a multisystemic zoonotic disease which in humans can involve the skin, joints, heart and/or nervous system. In this study a total of 11 patients with clinical manifestations have been assessed at the Institute for Occupational Health. Evaluation of the patients was done in order to determine their working capability and further professional orientation. Patients were of different gender, age, education and profession. They fulfilled at least two of the three criteria: tick infestation data (epidemiological criteria), central and/or peripheral neurological symptoms (clinical criteria) and a positive serological finding. Diagnosis was done upon classical clinical criteria: electromyeloneurography (EMNG) analysis, neurological impairments, electroencephalography (EEG), computer tomography (CT) and/or magnetic resonance imaging (MRI). IgM and IgG antibodies against B. burgdorferi were determined by commercial ELISA kits. IgM antibodies were recorded in the serum of 4 (44.4%) and IgG in 6 (66.7%) patients. Electro-myeloneurography findings of the upper and lower limbs were positive in 5 (83.3%), electroencephalography in 4 (66.6%) of the 6 observed patients and CT was positive in 4 (36.4%) of the 5 observed patients. The study has established that in patients with neuroborreliosis (NB) the capability to carry out intellectual tasks, as well as responsible duties is impaired due to poor memory. Patients suffering from peripheral neuropathies are not fit to withstand longterm walks, weight lifting and carrying or any other form of physical stress.
AB  - Lajm borelioza je multisistemsko oboljenje, iz grupe zoonoza koje kod ljudi može zahvatiti kožu, zglobove, srce i/ili nervni sistem. Istraživanjem je obuhvaćeno 11 bolesnika sa kliničkim manifestacijama neuroborelioze koji su ispitivani u Institutu za medicinu rada Srbije u cilju ocene radne sposobnosti i dalje profesionalne orijentacije. Ispitani su bolesnici različite starosti, pola, nivoa obrazovanja i različitih zanimanja koji su ispunili minimalno dva od tri kriterijuma i to: podatak o ubodu krpelja (epidemiološki kriterijum), ispoljavanje centralnih i/ili, perifernih neuroloških simptoma (klinički kriterijum) i pozitivan serološki nalaz. Dijagnoza neuroborelioze je postavljena na osnovu klasičnih kliničkih kriterijuma: neurološ kih ispada, analize elektro-mioneurografije (EMNG), elektroencefalografije (EEG), kompjuterske tomografije (CT) i/ili magnetne rezonance (MRI). Ispitivanje prisustva antitela IgM i IgG klase u krvnom serumu prema B. burgdorferi vršeno je komercijalnim ELISA testom. Antitela IgM klase registrovana su u serumu četiri (44,4%), dok su IgG antitela registrovana kod 6 (66,7%) ispitanih pacijenata. Nalaz elektro-mioneurografije gornjih i donjih ekstremiteta je bio pozitivan kod pet (83,3%), nalaz elektroencefalografije kod četiri (66,6%) od šest ispitanih pacijenata, dok je nalaz CT bio pozitivan kod 4 (36,4%) od pet ispitanih pacijenata. Sprovedenim ispitivanjem je utvrđeno da je kod bolesnika sa razvijenom neuroboreliozom smanjena sposobnost za bilo koju vrstu intelektualnog rada, kao i za poslove koji su povezani sa moralnom i materijalnom odgovornošću zbog problema sa pamćenjem. Kod bolesnika sa perifernim neuropatijama postoji nesposobnost za poslove koji uključuju dugotrajno stajanje i hodanje, dizanje i nošenje tereta, kao i bilo koju vrstu fizičkog rada.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Lyme neuroborreliosis
T1  - Lajm neuroborelioza
VL  - 61
IS  - 1
SP  - 89
EP  - 98
DO  - 10.2298/AVB1101089M
ER  - 

@article{
author = "Milovanović, Aleksandar and Milovanović, J. and Obrenović, Sonja and Milovanović, Anđela and Simonović, P. and Čemerikić, D. and Tačević, Z. and Petronić, Ivana and Grajić, M. and Kekuš, Divna and Popević, M.",
year = "2011",
abstract = "Lyme borreliosis (LB) is a multisystemic zoonotic disease which in humans can involve the skin, joints, heart and/or nervous system. In this study a total of 11 patients with clinical manifestations have been assessed at the Institute for Occupational Health. Evaluation of the patients was done in order to determine their working capability and further professional orientation. Patients were of different gender, age, education and profession. They fulfilled at least two of the three criteria: tick infestation data (epidemiological criteria), central and/or peripheral neurological symptoms (clinical criteria) and a positive serological finding. Diagnosis was done upon classical clinical criteria: electromyeloneurography (EMNG) analysis, neurological impairments, electroencephalography (EEG), computer tomography (CT) and/or magnetic resonance imaging (MRI). IgM and IgG antibodies against B. burgdorferi were determined by commercial ELISA kits. IgM antibodies were recorded in the serum of 4 (44.4%) and IgG in 6 (66.7%) patients. Electro-myeloneurography findings of the upper and lower limbs were positive in 5 (83.3%), electroencephalography in 4 (66.6%) of the 6 observed patients and CT was positive in 4 (36.4%) of the 5 observed patients. The study has established that in patients with neuroborreliosis (NB) the capability to carry out intellectual tasks, as well as responsible duties is impaired due to poor memory. Patients suffering from peripheral neuropathies are not fit to withstand longterm walks, weight lifting and carrying or any other form of physical stress., Lajm borelioza je multisistemsko oboljenje, iz grupe zoonoza koje kod ljudi može zahvatiti kožu, zglobove, srce i/ili nervni sistem. Istraživanjem je obuhvaćeno 11 bolesnika sa kliničkim manifestacijama neuroborelioze koji su ispitivani u Institutu za medicinu rada Srbije u cilju ocene radne sposobnosti i dalje profesionalne orijentacije. Ispitani su bolesnici različite starosti, pola, nivoa obrazovanja i različitih zanimanja koji su ispunili minimalno dva od tri kriterijuma i to: podatak o ubodu krpelja (epidemiološki kriterijum), ispoljavanje centralnih i/ili, perifernih neuroloških simptoma (klinički kriterijum) i pozitivan serološki nalaz. Dijagnoza neuroborelioze je postavljena na osnovu klasičnih kliničkih kriterijuma: neurološ kih ispada, analize elektro-mioneurografije (EMNG), elektroencefalografije (EEG), kompjuterske tomografije (CT) i/ili magnetne rezonance (MRI). Ispitivanje prisustva antitela IgM i IgG klase u krvnom serumu prema B. burgdorferi vršeno je komercijalnim ELISA testom. Antitela IgM klase registrovana su u serumu četiri (44,4%), dok su IgG antitela registrovana kod 6 (66,7%) ispitanih pacijenata. Nalaz elektro-mioneurografije gornjih i donjih ekstremiteta je bio pozitivan kod pet (83,3%), nalaz elektroencefalografije kod četiri (66,6%) od šest ispitanih pacijenata, dok je nalaz CT bio pozitivan kod 4 (36,4%) od pet ispitanih pacijenata. Sprovedenim ispitivanjem je utvrđeno da je kod bolesnika sa razvijenom neuroboreliozom smanjena sposobnost za bilo koju vrstu intelektualnog rada, kao i za poslove koji su povezani sa moralnom i materijalnom odgovornošću zbog problema sa pamćenjem. Kod bolesnika sa perifernim neuropatijama postoji nesposobnost za poslove koji uključuju dugotrajno stajanje i hodanje, dizanje i nošenje tereta, kao i bilo koju vrstu fizičkog rada.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Lyme neuroborreliosis, Lajm neuroborelioza",
volume = "61",
number = "1",
pages = "89-98",
doi = "10.2298/AVB1101089M"
}

Milovanović, A., Milovanović, J., Obrenović, S., Milovanović, A., Simonović, P., Čemerikić, D., Tačević, Z., Petronić, I., Grajić, M., Kekuš, D.,& Popević, M.. (2011). Lyme neuroborreliosis. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(1), 89-98.
https://doi.org/10.2298/AVB1101089M

Milovanović A, Milovanović J, Obrenović S, Milovanović A, Simonović P, Čemerikić D, Tačević Z, Petronić I, Grajić M, Kekuš D, Popević M. Lyme neuroborreliosis. in Acta Veterinaria-Beograd. 2011;61(1):89-98.
doi:10.2298/AVB1101089M .

Milovanović, Aleksandar, Milovanović, J., Obrenović, Sonja, Milovanović, Anđela, Simonović, P., Čemerikić, D., Tačević, Z., Petronić, Ivana, Grajić, M., Kekuš, Divna, Popević, M., "Lyme neuroborreliosis" in Acta Veterinaria-Beograd, 61, no. 1 (2011):89-98,
https://doi.org/10.2298/AVB1101089M . .

TY  - JOUR
AU  - Milovanović, Aleksandar
AU  - Jovičin, Milovan
AU  - Barna, Tomislav
AU  - Golubović, Snežana
AU  - Resanović, Vojislav
AU  - Rodić, Vladimir
AU  - Kitanović, Smiljka
AU  - Lazarević, Miodrag
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/626
AB  - In this work the possibility of producing and using our new EIA (enzyme immunosorbent test) set for direct determination of progesterone concentration in whole milk was analyzed. The test confirmed high values in milk of confirmed gravid cows (13,16± 13,29 ng/ml; n=10) and confirmed non-gravid cows (1,35±0,98 ng/ml; n=8). This progesterone test helps following variations of progesterone concentration in blood and milk cow samples taken every second day. The progesterone concentration of gravid and low values of non-gravid cows are displayed, as well as errors in cycles and mistakes in insemination. The results were compared to the results obtained by the RIA progesterone test done on blood sera samples. Progesterone concentration measured with EIA test in 15 milk samples was compared to the values of progesterone obtained by a commercial EIA test. The differences were not statistically significant (t-test, t-0.1144; p-0.9097). After milking, the samples were stored for 28 days at 4oC, conserved by potassium dichromate, and a loss of progesterone activity by 25,90±15,47% (4,69±2,90 ng/ml) was noticed, but in the final outcome this did not influence the clinical results. Accordingly, milk samples with preservative may be stored in a refrigerator for four weeks.
AB  - U ovom radu prikazani su rezultati primene našeg novog enzimskog imunosorbent testa (EIA) za direktno određivanje koncentracije progesterona u punom mleku krava. Testom su potvrđene visoke koncentracije u mleku kod steonih krava (13,16±13,29 ng/ml; n=10) i niske koncentracije kod nesteonih krava (1,35±0,98 ng/ml; n=8). Ovim progesteronskim testom se u uzorcima mleka mogu pouzdano pratiti variranja u koncentraciji progesterona po danima ciklusa krava, ako se uzorci uzimaju svakog drugog dana. U radu su prikazani i progesteronski profili steonih i nesteonih krava, kao i profili onih sa poremećenim ciklusom i sa greškom u osemenjavanju. Rezultati dobijeni EIA testom su upoređivani sa rezultatima dobijenim komercijalnim RIA-progesteronskim setom iz uzoraka krvnog seruma. Razlike u koncentraciji progesterona u 15 uzoraka mleka krava novoproizvedenim EIA testom i koncentraciji određivanoj komercijalnim EIA testom nisu bile statistički značajne (t-test: t-0,1144; p-0,9097). U uzorcima mleka čuvanim na temperaturi frižidera (+4oC) konzervisanim kalijum bihromatom (do 28. dana nakon muže) ustanovljene su niže koncentracije progesterona za 25,90±15,47% (4,69± 2,90 ng/ml) u odnosu na koncentraciju na dan muže, što u krajnjem ishodu ipak nije uticalo na dobijene kliničke rezultate. Za primenu u EIA testu uzorci mleka sa konzervansom mogu se skladištiti četiri nedelje u frižideru.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Applying enzyme immunotest for determination of progesterone concentration in whole cow 's milk
T1  - Primena enzimskog imunotesta za određivanje koncentracije progesterona u punom kravljem mleku
VL  - 63
IS  - 1-2
SP  - 45
EP  - 59
DO  - 10.2298/VETGL0902045M
ER  - 

@article{
author = "Milovanović, Aleksandar and Jovičin, Milovan and Barna, Tomislav and Golubović, Snežana and Resanović, Vojislav and Rodić, Vladimir and Kitanović, Smiljka and Lazarević, Miodrag",
year = "2009",
abstract = "In this work the possibility of producing and using our new EIA (enzyme immunosorbent test) set for direct determination of progesterone concentration in whole milk was analyzed. The test confirmed high values in milk of confirmed gravid cows (13,16± 13,29 ng/ml; n=10) and confirmed non-gravid cows (1,35±0,98 ng/ml; n=8). This progesterone test helps following variations of progesterone concentration in blood and milk cow samples taken every second day. The progesterone concentration of gravid and low values of non-gravid cows are displayed, as well as errors in cycles and mistakes in insemination. The results were compared to the results obtained by the RIA progesterone test done on blood sera samples. Progesterone concentration measured with EIA test in 15 milk samples was compared to the values of progesterone obtained by a commercial EIA test. The differences were not statistically significant (t-test, t-0.1144; p-0.9097). After milking, the samples were stored for 28 days at 4oC, conserved by potassium dichromate, and a loss of progesterone activity by 25,90±15,47% (4,69±2,90 ng/ml) was noticed, but in the final outcome this did not influence the clinical results. Accordingly, milk samples with preservative may be stored in a refrigerator for four weeks., U ovom radu prikazani su rezultati primene našeg novog enzimskog imunosorbent testa (EIA) za direktno određivanje koncentracije progesterona u punom mleku krava. Testom su potvrđene visoke koncentracije u mleku kod steonih krava (13,16±13,29 ng/ml; n=10) i niske koncentracije kod nesteonih krava (1,35±0,98 ng/ml; n=8). Ovim progesteronskim testom se u uzorcima mleka mogu pouzdano pratiti variranja u koncentraciji progesterona po danima ciklusa krava, ako se uzorci uzimaju svakog drugog dana. U radu su prikazani i progesteronski profili steonih i nesteonih krava, kao i profili onih sa poremećenim ciklusom i sa greškom u osemenjavanju. Rezultati dobijeni EIA testom su upoređivani sa rezultatima dobijenim komercijalnim RIA-progesteronskim setom iz uzoraka krvnog seruma. Razlike u koncentraciji progesterona u 15 uzoraka mleka krava novoproizvedenim EIA testom i koncentraciji određivanoj komercijalnim EIA testom nisu bile statistički značajne (t-test: t-0,1144; p-0,9097). U uzorcima mleka čuvanim na temperaturi frižidera (+4oC) konzervisanim kalijum bihromatom (do 28. dana nakon muže) ustanovljene su niže koncentracije progesterona za 25,90±15,47% (4,69± 2,90 ng/ml) u odnosu na koncentraciju na dan muže, što u krajnjem ishodu ipak nije uticalo na dobijene kliničke rezultate. Za primenu u EIA testu uzorci mleka sa konzervansom mogu se skladištiti četiri nedelje u frižideru.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Applying enzyme immunotest for determination of progesterone concentration in whole cow 's milk, Primena enzimskog imunotesta za određivanje koncentracije progesterona u punom kravljem mleku",
volume = "63",
number = "1-2",
pages = "45-59",
doi = "10.2298/VETGL0902045M"
}

Milovanović, A., Jovičin, M., Barna, T., Golubović, S., Resanović, V., Rodić, V., Kitanović, S.,& Lazarević, M.. (2009). Applying enzyme immunotest for determination of progesterone concentration in whole cow 's milk. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(1-2), 45-59.
https://doi.org/10.2298/VETGL0902045M

Milovanović A, Jovičin M, Barna T, Golubović S, Resanović V, Rodić V, Kitanović S, Lazarević M. Applying enzyme immunotest for determination of progesterone concentration in whole cow 's milk. in Veterinarski Glasnik. 2009;63(1-2):45-59.
doi:10.2298/VETGL0902045M .

Milovanović, Aleksandar, Jovičin, Milovan, Barna, Tomislav, Golubović, Snežana, Resanović, Vojislav, Rodić, Vladimir, Kitanović, Smiljka, Lazarević, Miodrag, "Applying enzyme immunotest for determination of progesterone concentration in whole cow 's milk" in Veterinarski Glasnik, 63, no. 1-2 (2009):45-59,
https://doi.org/10.2298/VETGL0902045M . .

TY  - JOUR
AU  - Milovanović, Aleksandar
AU  - Lazarević, Miodrag
AU  - Milanović, Svetlana
AU  - Kirovski, Danijela
AU  - Jovičin, Milovan
PY  - 2005
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/361
AB  - The aim of this study was to determine titers of antisperm antibodies (ASA) of Ig G and Ig A class in sera and cervical mucus of artificially inseminated Holstein cows in order to correlate these results with the duration of the open days period. Investigations were conducted on a total of 181 cows originating from three different dairy farms. Blood and cervical mucus samples for laboratory analyses were collected on the day of the last artificial insemination. Presence of ASA was determined by indirect immunofluorescence method (IIF) using bulls' sperm cells prepared for artificial insemination by suspending in TRIS - egg yolk or "Biociphos + " extenders prior to deep freezing. Our results strongly confirm the hypothesis that immune mechanisms may be involved in reproductive disturbances due to high levels of ASA of lg A class. In the sera and cervical mucus of cows, high levels of ASA were found in animals with longer open days period. In this study we were not able to demonstrate differences in ASA titers when sperm cells were suspended in different extenders and used for the IIF test.
AB  - U ovom radu su izneti rezultati ispitivanja titra antitela Ig G i Ig A klase poreklom iz krvnog seruma i cervikalne sluzi veštački osemenjavanih holštajn krava protiv antigena spermatozoida bika. Ispitivanja su izvedena na ukupnom broju od 181 plotkinje sa tri regionalne farme muznih krava. Uzorci krvi i cervikalne sluzi su prikupljeni na dan poslednjeg veštačkog osemenjavanja. Titar antitela protiv antigena spermatozoida (ASA) je određivan metodom indirektne imunofluorescence a za izvođenje testa su korišćeni spermatozoidi suspendovani u TRIS žumanjčanom ili "Biociphos +" razređivaču. Naši rezultati potvrđuju hipotezu da su imunski mehanizmi uključeni u nastanak nekih reproduktivnih poremećaja jer su plotkinje sa visokim titrom ASA u serumu i cervikalnoj sluzi imala duži servis period. Osim toga, u ovim ispitivanjima nismo utvrdili postojanje razlika u titru ASA u zavisnosti od vrste korišćenog razređivača.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Open days period and antispermatozoal antibodies in artificially inseminated cows
T1  - Servis period i antitela protiv spermatozoida kod veštački osemenjavanih krava
VL  - 55
IS  - 5-6
SP  - 449
EP  - 460
DO  - 10.2298/AVB0506449M
ER  - 

@article{
author = "Milovanović, Aleksandar and Lazarević, Miodrag and Milanović, Svetlana and Kirovski, Danijela and Jovičin, Milovan",
year = "2005",
abstract = "The aim of this study was to determine titers of antisperm antibodies (ASA) of Ig G and Ig A class in sera and cervical mucus of artificially inseminated Holstein cows in order to correlate these results with the duration of the open days period. Investigations were conducted on a total of 181 cows originating from three different dairy farms. Blood and cervical mucus samples for laboratory analyses were collected on the day of the last artificial insemination. Presence of ASA was determined by indirect immunofluorescence method (IIF) using bulls' sperm cells prepared for artificial insemination by suspending in TRIS - egg yolk or "Biociphos + " extenders prior to deep freezing. Our results strongly confirm the hypothesis that immune mechanisms may be involved in reproductive disturbances due to high levels of ASA of lg A class. In the sera and cervical mucus of cows, high levels of ASA were found in animals with longer open days period. In this study we were not able to demonstrate differences in ASA titers when sperm cells were suspended in different extenders and used for the IIF test., U ovom radu su izneti rezultati ispitivanja titra antitela Ig G i Ig A klase poreklom iz krvnog seruma i cervikalne sluzi veštački osemenjavanih holštajn krava protiv antigena spermatozoida bika. Ispitivanja su izvedena na ukupnom broju od 181 plotkinje sa tri regionalne farme muznih krava. Uzorci krvi i cervikalne sluzi su prikupljeni na dan poslednjeg veštačkog osemenjavanja. Titar antitela protiv antigena spermatozoida (ASA) je određivan metodom indirektne imunofluorescence a za izvođenje testa su korišćeni spermatozoidi suspendovani u TRIS žumanjčanom ili "Biociphos +" razređivaču. Naši rezultati potvrđuju hipotezu da su imunski mehanizmi uključeni u nastanak nekih reproduktivnih poremećaja jer su plotkinje sa visokim titrom ASA u serumu i cervikalnoj sluzi imala duži servis period. Osim toga, u ovim ispitivanjima nismo utvrdili postojanje razlika u titru ASA u zavisnosti od vrste korišćenog razređivača.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Open days period and antispermatozoal antibodies in artificially inseminated cows, Servis period i antitela protiv spermatozoida kod veštački osemenjavanih krava",
volume = "55",
number = "5-6",
pages = "449-460",
doi = "10.2298/AVB0506449M"
}

Milovanović, A., Lazarević, M., Milanović, S., Kirovski, D.,& Jovičin, M.. (2005). Open days period and antispermatozoal antibodies in artificially inseminated cows. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 55(5-6), 449-460.
https://doi.org/10.2298/AVB0506449M

Milovanović A, Lazarević M, Milanović S, Kirovski D, Jovičin M. Open days period and antispermatozoal antibodies in artificially inseminated cows. in Acta Veterinaria-Beograd. 2005;55(5-6):449-460.
doi:10.2298/AVB0506449M .

Milovanović, Aleksandar, Lazarević, Miodrag, Milanović, Svetlana, Kirovski, Danijela, Jovičin, Milovan, "Open days period and antispermatozoal antibodies in artificially inseminated cows" in Acta Veterinaria-Beograd, 55, no. 5-6 (2005):449-460,
https://doi.org/10.2298/AVB0506449M . .

TY  - JOUR
AU  - Lazarević, Miodrag
AU  - Milovanović, Aleksandar
AU  - Milanović, Svetlana
AU  - Kirovski, Danijela
AU  - Ilić, Vojislav
PY  - 2004
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/277
AB  - In this study the relationship was explored between general immune reactivity, as estimated by the cutaneous basophil hypersensitivity reaction (CBHR) to phytohemagglutinin (PHA), and the level of anti-sperm antibodies of the Ig A and Ig G class in the cervical mucus and sera of cows with different reproductive results. The hypersensitivity test was performed on the day of artificial insemination when cervical mucus and sera samples were also collected. The animals were divided into groups according to the total number of inseminations, number of inseminations per calving and their age. The titer of antibodies was determined by the indirect immunofluorescence method using sperm cells suspended in Tris egg-yolk extender. Our results indicate that the intensity of CBHR is lowest in cows inseminated once or twice and in younger animals, while, there were no differences between the groups when they were formed according to the number of inseminations per calving. Titers of antisperm antibodies (ASA) of the Ig A class in cervical mucus (CM) increased with the number of artificial inseminations, number of inseminations per calving and age of the cows.
AB  - Cilj ovih ispitivanja je bio da se utvrdi odnos između rezultata testa kožne preosetljivosti na fitohemaglutinin, nekih reproduktivnih pokazatelja i koncentracije antispermatozoalnih antitela Ig A klase u cervikalnoj sluzi krava koje se veštački osemenjavaju. Test kožne preosetljivosti je izvođen na dan veštačkog osemenjavanja a istog dana su prikupljani i uzorci cervikalne sluzi. Krave su bile podeljene u grupe na osnovu broja ukupnih prethodnih osemenjavanja, prosečnog broja osemenjavanja po graviditetu i na osnovu starosti. Prisustvo antispermatozoalnih antitela je određivano metodom indirektne imunofluorescence korišćenjem spermatozoida suspendovanih u Tris-žumanjčanom razređivaču. Postignuti rezultati ukazuju da je intenzitet kožne preosetljivosti na PHA manji kod krava koje su osemenjene 1-2 puta kao i kod mlađih jedinki. Intenzitet ove reakcije se nije razlikovao kod krava koje zahtevaju manje ili više pokušaja VO po teljenju. Takođe je dokazano da se titar antispermatozoalnih Ig A antitela u cervikalnoj sluzi povećava sa ukupnim brojem osemenjavanja, brojem osemenjavanja po teljenju i starošću plotkinja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Cutaneous basophil hypersensitivity reaction to phytohemagglutinin in repeat breeder cows
T1  - Test kožne preosetljivosti na fitohemaglutinin (PHA) kod krava koje povađaju
VL  - 54
IS  - 5-6
SP  - 337
EP  - 346
DO  - 10.2298/AVB0406337L
ER  - 

@article{
author = "Lazarević, Miodrag and Milovanović, Aleksandar and Milanović, Svetlana and Kirovski, Danijela and Ilić, Vojislav",
year = "2004",
abstract = "In this study the relationship was explored between general immune reactivity, as estimated by the cutaneous basophil hypersensitivity reaction (CBHR) to phytohemagglutinin (PHA), and the level of anti-sperm antibodies of the Ig A and Ig G class in the cervical mucus and sera of cows with different reproductive results. The hypersensitivity test was performed on the day of artificial insemination when cervical mucus and sera samples were also collected. The animals were divided into groups according to the total number of inseminations, number of inseminations per calving and their age. The titer of antibodies was determined by the indirect immunofluorescence method using sperm cells suspended in Tris egg-yolk extender. Our results indicate that the intensity of CBHR is lowest in cows inseminated once or twice and in younger animals, while, there were no differences between the groups when they were formed according to the number of inseminations per calving. Titers of antisperm antibodies (ASA) of the Ig A class in cervical mucus (CM) increased with the number of artificial inseminations, number of inseminations per calving and age of the cows., Cilj ovih ispitivanja je bio da se utvrdi odnos između rezultata testa kožne preosetljivosti na fitohemaglutinin, nekih reproduktivnih pokazatelja i koncentracije antispermatozoalnih antitela Ig A klase u cervikalnoj sluzi krava koje se veštački osemenjavaju. Test kožne preosetljivosti je izvođen na dan veštačkog osemenjavanja a istog dana su prikupljani i uzorci cervikalne sluzi. Krave su bile podeljene u grupe na osnovu broja ukupnih prethodnih osemenjavanja, prosečnog broja osemenjavanja po graviditetu i na osnovu starosti. Prisustvo antispermatozoalnih antitela je određivano metodom indirektne imunofluorescence korišćenjem spermatozoida suspendovanih u Tris-žumanjčanom razređivaču. Postignuti rezultati ukazuju da je intenzitet kožne preosetljivosti na PHA manji kod krava koje su osemenjene 1-2 puta kao i kod mlađih jedinki. Intenzitet ove reakcije se nije razlikovao kod krava koje zahtevaju manje ili više pokušaja VO po teljenju. Takođe je dokazano da se titar antispermatozoalnih Ig A antitela u cervikalnoj sluzi povećava sa ukupnim brojem osemenjavanja, brojem osemenjavanja po teljenju i starošću plotkinja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Cutaneous basophil hypersensitivity reaction to phytohemagglutinin in repeat breeder cows, Test kožne preosetljivosti na fitohemaglutinin (PHA) kod krava koje povađaju",
volume = "54",
number = "5-6",
pages = "337-346",
doi = "10.2298/AVB0406337L"
}

Lazarević, M., Milovanović, A., Milanović, S., Kirovski, D.,& Ilić, V.. (2004). Cutaneous basophil hypersensitivity reaction to phytohemagglutinin in repeat breeder cows. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 54(5-6), 337-346.
https://doi.org/10.2298/AVB0406337L

Lazarević M, Milovanović A, Milanović S, Kirovski D, Ilić V. Cutaneous basophil hypersensitivity reaction to phytohemagglutinin in repeat breeder cows. in Acta Veterinaria-Beograd. 2004;54(5-6):337-346.
doi:10.2298/AVB0406337L .

Lazarević, Miodrag, Milovanović, Aleksandar, Milanović, Svetlana, Kirovski, Danijela, Ilić, Vojislav, "Cutaneous basophil hypersensitivity reaction to phytohemagglutinin in repeat breeder cows" in Acta Veterinaria-Beograd, 54, no. 5-6 (2004):337-346,
https://doi.org/10.2298/AVB0406337L . .

TY  - JOUR
AU  - Jovičin, Milovan
AU  - Petrujkić, Tihomir
AU  - Milovanović, Aleksandar
AU  - Dujaković, Milorad
AU  - Žikić, Dragan
PY  - 2003
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1889
AB  - U radu je prikazana analiza učestalosti intervala između dva osemenjavanja u dva godišnja perioda, od 1. 4. 2001. do 31. 3. 2003. godine na farmi sa 500 crno-belih holštajn-frizijskih krava, sa proizvodnjom mleka od 5500 do 6000 kg mleka. Histogramima su predstavljeni ciklusi od po mesec i po dana sa polnim ciklusima kod steonih, nesteonih i isključenih krava. Normalni i višestruki polni ciklusi ukazuju na greške u otkrivanju estrusa, kod 26,70 posto / 22,73 posto i kod 33,25 posto / 33,77 posto krava, u prvom / drugom analiziranom periodu. Skraćeni i produženi polni ciklusi zabeleženi su kod 12,22 posto i 4,34 posto, odnosno kod 10,07 posto i 7,36 posto krava i junica, a predstavljaju indikator poreme}aja u ishrani i držanju, odnosno pokazatelj su povećanog stepena embrionalnog mortaliteta. Poremećeni polni ciklusi su ustanovljeni kod 41,31 posto i 43,10 posto krava i junica i kumulacija su ostalih činilaca stadnog steriliteta. Planskim radom na prevenciji poremećaja funkcije jajnika i dobrom evidencijom, postiže se bolja plodnost zapata.
AB  - The frequency of interestrus intervals between two inseminations during two annual period, from 1. 4. 2001 - 31. 3. 2003, on a farm with 500 Holstein-Frisian cows and milk production of 5500-6000 kg was analyzed. Periods of 1½ months of estrus cycles were presented by histograms for pregnant, nonpregnant and culled cows. Normal and multiple cycles indicate estrus detection errors, and were recorded in 26.70% / 22.73% and 33.25% / 33.77% cows, in the first and second analysed periods, respectively. Short and prolonged estrus cycles were noted in 12.22% / 4.34%, and 10.07% / 7.36% cows, in the first and second analysed periods respectively, and indicate nutritional disturbances and embryonal mortality. Disturbed estrus periods were found in 41.31% and 43.10% cows and heifers and were a consequence of other cumulate factors in herd sterility. Herd fertility improvement may be achieved with systematic work on prevention of ovarial disfunction and proper records management
PB  - Fakultet veterinarske medicine, Beograd, Srbija
T2  - Veterinarski Glasnik
T1  - Analysis of factors influencing estrus cycle in dairy cows
T1  - Analiza faktora koji utiču na tok estralnog ciklusa visokomlečnih krava
VL  - 57
IS  - 3-4
SP  - 209
EP  - 223
DO  - 10.2298/VETGL0304209J
ER  - 

@article{
author = "Jovičin, Milovan and Petrujkić, Tihomir and Milovanović, Aleksandar and Dujaković, Milorad and Žikić, Dragan",
year = "2003",
abstract = "U radu je prikazana analiza učestalosti intervala između dva osemenjavanja u dva godišnja perioda, od 1. 4. 2001. do 31. 3. 2003. godine na farmi sa 500 crno-belih holštajn-frizijskih krava, sa proizvodnjom mleka od 5500 do 6000 kg mleka. Histogramima su predstavljeni ciklusi od po mesec i po dana sa polnim ciklusima kod steonih, nesteonih i isključenih krava. Normalni i višestruki polni ciklusi ukazuju na greške u otkrivanju estrusa, kod 26,70 posto / 22,73 posto i kod 33,25 posto / 33,77 posto krava, u prvom / drugom analiziranom periodu. Skraćeni i produženi polni ciklusi zabeleženi su kod 12,22 posto i 4,34 posto, odnosno kod 10,07 posto i 7,36 posto krava i junica, a predstavljaju indikator poreme}aja u ishrani i držanju, odnosno pokazatelj su povećanog stepena embrionalnog mortaliteta. Poremećeni polni ciklusi su ustanovljeni kod 41,31 posto i 43,10 posto krava i junica i kumulacija su ostalih činilaca stadnog steriliteta. Planskim radom na prevenciji poremećaja funkcije jajnika i dobrom evidencijom, postiže se bolja plodnost zapata., The frequency of interestrus intervals between two inseminations during two annual period, from 1. 4. 2001 - 31. 3. 2003, on a farm with 500 Holstein-Frisian cows and milk production of 5500-6000 kg was analyzed. Periods of 1½ months of estrus cycles were presented by histograms for pregnant, nonpregnant and culled cows. Normal and multiple cycles indicate estrus detection errors, and were recorded in 26.70% / 22.73% and 33.25% / 33.77% cows, in the first and second analysed periods, respectively. Short and prolonged estrus cycles were noted in 12.22% / 4.34%, and 10.07% / 7.36% cows, in the first and second analysed periods respectively, and indicate nutritional disturbances and embryonal mortality. Disturbed estrus periods were found in 41.31% and 43.10% cows and heifers and were a consequence of other cumulate factors in herd sterility. Herd fertility improvement may be achieved with systematic work on prevention of ovarial disfunction and proper records management",
publisher = "Fakultet veterinarske medicine, Beograd, Srbija",
journal = "Veterinarski Glasnik",
title = "Analysis of factors influencing estrus cycle in dairy cows, Analiza faktora koji utiču na tok estralnog ciklusa visokomlečnih krava",
volume = "57",
number = "3-4",
pages = "209-223",
doi = "10.2298/VETGL0304209J"
}

Jovičin, M., Petrujkić, T., Milovanović, A., Dujaković, M.,& Žikić, D.. (2003). Analysis of factors influencing estrus cycle in dairy cows. in Veterinarski Glasnik
Fakultet veterinarske medicine, Beograd, Srbija., 57(3-4), 209-223.
https://doi.org/10.2298/VETGL0304209J

Jovičin M, Petrujkić T, Milovanović A, Dujaković M, Žikić D. Analysis of factors influencing estrus cycle in dairy cows. in Veterinarski Glasnik. 2003;57(3-4):209-223.
doi:10.2298/VETGL0304209J .

Jovičin, Milovan, Petrujkić, Tihomir, Milovanović, Aleksandar, Dujaković, Milorad, Žikić, Dragan, "Analysis of factors influencing estrus cycle in dairy cows" in Veterinarski Glasnik, 57, no. 3-4 (2003):209-223,
https://doi.org/10.2298/VETGL0304209J . .

TY  - JOUR
AU  - Lazarević, Miodrag
AU  - Milanović, Svetlana
AU  - Kirovski, Danijela
AU  - Milovanović, Aleksandar
PY  - 2003
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/245
AB  - The aim of this study was to investigate the level of anti-sperm antibodies of the Ig A class in the cervical mucus and sera of artificially inseminated cows. Cervical mucus and sera samples were collected on the day of artificial insemination and the animals were divided into four groups of 20 cows each according to the number of previous inseminations. The titer of antibodies was determined by the indirect immunofluorescence method using sperm cells suspended in Tris egg-yolk extender or the commercial extender "Biociphos +". Our results indicate that titers of antisperm antibodies of the Ig A class elevate with the number of artificial inseminations. Mean titer values were higher in the cervical mucus samples than in sera indicating that the local immune response is more relevant for the immunological reactivity to sperm and extender antigens. In addition, the titer of antisperm antibodies was generally higher when sperm cells suspended in Tris egg-yolk extender were used for the test.
AB  - Cilj ovih ispitivanja je bio da se utvrdi nivo antispermatozoalnih antitela Ig A klase u krvnom serumu i cervikalnoj sluzi krava koje se veštački osemenjavaju. Uzorci krvi i cervikalne sluzi su prikupljani na dan veštačkog osemenjavanja a životinje su bile podeljebne u četiri grupe na osnovu broja prethodnih osemenjavanja. Prisustvo antitspermatozoalnih antitela je određivano metodom indirektne imunofluorescence korišćenjem spermatozoida suspendovanih u Tris-žumanjčanom razređivaču i komercijalnom razređivaču "Biociphos +". Postignuti rezulati ukazuju da titar antispermatozoalnih Ig A antitela raste sa brojem osemenjavanja i ima veće vrednosti u cervikalnoj sluzi nego u serumu. Ovi rezultati potvrđuju hipotezu o značaju lokalnog imunskog odgovora na antigene spermatozoida i razređivača za spermu bika. Osim toga titar antitela je imao veće vrednosti kada su za izvođenje testa korišćeni spermatozoidi suspendovani u Tris-žumanjčanom razređivaču.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Antisperm antibodies of the Ig a class in the cervical mucus and sera of artificially inseminated cows
T1  - Ig A antititela protiv spermatozoida u cervikalnoj sluzi i serumu krava koje se veštački osemenjavaju
VL  - 53
IS  - 5-6
SP  - 311
EP  - 320
DO  - 10.2298/AVB0306311L
ER  - 

@article{
author = "Lazarević, Miodrag and Milanović, Svetlana and Kirovski, Danijela and Milovanović, Aleksandar",
year = "2003",
abstract = "The aim of this study was to investigate the level of anti-sperm antibodies of the Ig A class in the cervical mucus and sera of artificially inseminated cows. Cervical mucus and sera samples were collected on the day of artificial insemination and the animals were divided into four groups of 20 cows each according to the number of previous inseminations. The titer of antibodies was determined by the indirect immunofluorescence method using sperm cells suspended in Tris egg-yolk extender or the commercial extender "Biociphos +". Our results indicate that titers of antisperm antibodies of the Ig A class elevate with the number of artificial inseminations. Mean titer values were higher in the cervical mucus samples than in sera indicating that the local immune response is more relevant for the immunological reactivity to sperm and extender antigens. In addition, the titer of antisperm antibodies was generally higher when sperm cells suspended in Tris egg-yolk extender were used for the test., Cilj ovih ispitivanja je bio da se utvrdi nivo antispermatozoalnih antitela Ig A klase u krvnom serumu i cervikalnoj sluzi krava koje se veštački osemenjavaju. Uzorci krvi i cervikalne sluzi su prikupljani na dan veštačkog osemenjavanja a životinje su bile podeljebne u četiri grupe na osnovu broja prethodnih osemenjavanja. Prisustvo antitspermatozoalnih antitela je određivano metodom indirektne imunofluorescence korišćenjem spermatozoida suspendovanih u Tris-žumanjčanom razređivaču i komercijalnom razređivaču "Biociphos +". Postignuti rezulati ukazuju da titar antispermatozoalnih Ig A antitela raste sa brojem osemenjavanja i ima veće vrednosti u cervikalnoj sluzi nego u serumu. Ovi rezultati potvrđuju hipotezu o značaju lokalnog imunskog odgovora na antigene spermatozoida i razređivača za spermu bika. Osim toga titar antitela je imao veće vrednosti kada su za izvođenje testa korišćeni spermatozoidi suspendovani u Tris-žumanjčanom razređivaču.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Antisperm antibodies of the Ig a class in the cervical mucus and sera of artificially inseminated cows, Ig A antititela protiv spermatozoida u cervikalnoj sluzi i serumu krava koje se veštački osemenjavaju",
volume = "53",
number = "5-6",
pages = "311-320",
doi = "10.2298/AVB0306311L"
}

Lazarević, M., Milanović, S., Kirovski, D.,& Milovanović, A.. (2003). Antisperm antibodies of the Ig a class in the cervical mucus and sera of artificially inseminated cows. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 53(5-6), 311-320.
https://doi.org/10.2298/AVB0306311L

Lazarević M, Milanović S, Kirovski D, Milovanović A. Antisperm antibodies of the Ig a class in the cervical mucus and sera of artificially inseminated cows. in Acta Veterinaria-Beograd. 2003;53(5-6):311-320.
doi:10.2298/AVB0306311L .

Lazarević, Miodrag, Milanović, Svetlana, Kirovski, Danijela, Milovanović, Aleksandar, "Antisperm antibodies of the Ig a class in the cervical mucus and sera of artificially inseminated cows" in Acta Veterinaria-Beograd, 53, no. 5-6 (2003):311-320,
https://doi.org/10.2298/AVB0306311L . .

TY  - JOUR
AU  - Lazarević, Miodrag
AU  - Kirovski, Danijela
AU  - Fratrić, Natalija
AU  - Milanović, Svetlana
AU  - Jakovljević, Goran
AU  - Milovanović, Aleksandar
PY  - 2002
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/195
AB  - In this study we investigated the presence of antisperm antibodies in the sera of neonatal and young calves up to the age of 120 days by indirect immunofluorescence assay (IIP) and the sperm-agglutination method. By IIP assay, anil IgG and ants IgM antibodies were detected. In the sera of neonatal calves, before colostrum ingestion, no anti-sperm antibodies were detected due to physiological agammaglobulinemia. Titer obtained values in two day old and older calves were different for native sperm cells and sperm cells previously suspended in TRIS egg yolk extender or Biociphos plus extender (IMV, France) indicating antigenic differences between them. Their liter increased with age. This study confirmed the higher sensitivity of the IIP assay. We have also confirmed the hypothesis that in calves, antisperm antibodies naturally occurring before puberty, are most probably the result of cross reactivity with microbial antigens.
AB  - U ovom radu su izneti rezultati ispitivanja prisustva antitela protiv spermatozoida bika u serumu neonatalne teladi i teladi uzrasta do 120 dana metodama indirektne imunofluorescencije i aglutinacije u želatinu. Za izvođenje testova su korišćeni nativni spermatozoidi iz svežih ejakulata kao i spermatozoidi iz pajeta za veštačko osemenjavanje prethodno suspendovani u TRIS-žumanjčanom razređivaču ili razređivaču Biociphos plus (IMV. France). U serumu teladi pre ingestije kolostruma nisu dokazana antitela što je u skladu sa fiziološkom agamaglobulinemijom kod ove životinjske vrste. Kod teladi stare dva dana dokazano je prisustvo antitela protiv spermatozoida poreklom iz kolostruma i to u različitom titru u odnosu na vrstu korišćenih spermatozoida što ukazuje na njihovu antigensku različitost. Titar antitela se povećavao sa starošću teladi. Ova ispitivanja su potvrdila veću osetljivost indirektne imunofluorescence u odnosu na aglutinaciju. Na osnovu dobijenih rezultata se može pretpostaviti da su kod teladi, prirodna antitela na antigene spermatozoida nastala kao posledica unakrsne reaktivnosti sa mikrobijalnim antigenima.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The presence of naturally occurring antisperm antibodies in the sera of prepubertal calves
T1  - Prirodna antitela protiv spermatozoida u serumu teladi pre puberteta
VL  - 52
IS  - 5-6
SP  - 311
EP  - 318
DO  - 10.2298/AVB0206311L
ER  - 

@article{
author = "Lazarević, Miodrag and Kirovski, Danijela and Fratrić, Natalija and Milanović, Svetlana and Jakovljević, Goran and Milovanović, Aleksandar",
year = "2002",
abstract = "In this study we investigated the presence of antisperm antibodies in the sera of neonatal and young calves up to the age of 120 days by indirect immunofluorescence assay (IIP) and the sperm-agglutination method. By IIP assay, anil IgG and ants IgM antibodies were detected. In the sera of neonatal calves, before colostrum ingestion, no anti-sperm antibodies were detected due to physiological agammaglobulinemia. Titer obtained values in two day old and older calves were different for native sperm cells and sperm cells previously suspended in TRIS egg yolk extender or Biociphos plus extender (IMV, France) indicating antigenic differences between them. Their liter increased with age. This study confirmed the higher sensitivity of the IIP assay. We have also confirmed the hypothesis that in calves, antisperm antibodies naturally occurring before puberty, are most probably the result of cross reactivity with microbial antigens., U ovom radu su izneti rezultati ispitivanja prisustva antitela protiv spermatozoida bika u serumu neonatalne teladi i teladi uzrasta do 120 dana metodama indirektne imunofluorescencije i aglutinacije u želatinu. Za izvođenje testova su korišćeni nativni spermatozoidi iz svežih ejakulata kao i spermatozoidi iz pajeta za veštačko osemenjavanje prethodno suspendovani u TRIS-žumanjčanom razređivaču ili razređivaču Biociphos plus (IMV. France). U serumu teladi pre ingestije kolostruma nisu dokazana antitela što je u skladu sa fiziološkom agamaglobulinemijom kod ove životinjske vrste. Kod teladi stare dva dana dokazano je prisustvo antitela protiv spermatozoida poreklom iz kolostruma i to u različitom titru u odnosu na vrstu korišćenih spermatozoida što ukazuje na njihovu antigensku različitost. Titar antitela se povećavao sa starošću teladi. Ova ispitivanja su potvrdila veću osetljivost indirektne imunofluorescence u odnosu na aglutinaciju. Na osnovu dobijenih rezultata se može pretpostaviti da su kod teladi, prirodna antitela na antigene spermatozoida nastala kao posledica unakrsne reaktivnosti sa mikrobijalnim antigenima.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The presence of naturally occurring antisperm antibodies in the sera of prepubertal calves, Prirodna antitela protiv spermatozoida u serumu teladi pre puberteta",
volume = "52",
number = "5-6",
pages = "311-318",
doi = "10.2298/AVB0206311L"
}

Lazarević, M., Kirovski, D., Fratrić, N., Milanović, S., Jakovljević, G.,& Milovanović, A.. (2002). The presence of naturally occurring antisperm antibodies in the sera of prepubertal calves. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 52(5-6), 311-318.
https://doi.org/10.2298/AVB0206311L

Lazarević M, Kirovski D, Fratrić N, Milanović S, Jakovljević G, Milovanović A. The presence of naturally occurring antisperm antibodies in the sera of prepubertal calves. in Acta Veterinaria-Beograd. 2002;52(5-6):311-318.
doi:10.2298/AVB0206311L .

Lazarević, Miodrag, Kirovski, Danijela, Fratrić, Natalija, Milanović, Svetlana, Jakovljević, Goran, Milovanović, Aleksandar, "The presence of naturally occurring antisperm antibodies in the sera of prepubertal calves" in Acta Veterinaria-Beograd, 52, no. 5-6 (2002):311-318,
https://doi.org/10.2298/AVB0206311L . .