TY  - JOUR
AU  - Knežević, Dragan
AU  - Bošnjaković, Dušan
AU  - Dražić, Slavica
AU  - Nedić, Sreten
AU  - Vujanac, Ivan
AU  - Valčić, Olivera
AU  - Pantelić, Marija
AU  - Stojiljković, Mojca
AU  - Sladojević, Željko
AU  - Kirovski, Danijela
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3766
AB  - The study aimed to determine the concentration of energy-related hormones in cow’s
milk and to consider them from a public health perspective. Fourteen Holstein cows
were subjected to milk and blood sampling on the following days in lactation (DIL):
10, 30, 60, 90, 150, 180, 210, 250 and 280 to determine milk hormones, fat and protein
content and blood biochemical parameters. For the same purpose, bulk-tank milk
was sampled and samples of retail milk with 1.5% (CM1.5) and 3.2% (CM3.2) fat was
purchased. Milk insulin-like growth factor-1 (IGF-1) values were significantly lower at
90, 150, 180, 210 and 250 and significantly higher at 10, 30 and 60 DIL than lactation
average (LA). Milk insulin concentrations were significantly lower at 30, 60 and 90 and
higher at 210, 250 and 280 DIL than LA. Free thyroxine (fT4) level in the milk was
higher at 250 DIL, while milk free triiodothyronine (fT3) concentrations were lower
at 30, 60, 90 and 280 DIL, and significantly higher at 10 and 180 DIL than respective
LA. Milk cortisol levels were lower at 60 and 280 DIL than LA. All measured milk
hormones were significantly lower in CM1.5 compared to CM3.2, bulk-tank milk and
LA. An exception was the LA of IGF-1, which was significantly lower than the IGF-
1 content in CM1.5. Blood biochemical parameters fluctuated evenly during lactation
and were within the reference range. Hormone concentrations in cow’s milk fluctuate
during lactation, giving milk an important role in the context of public health.
PB  - Beograd : Fakultet veterinarske medicine
T2  - Acta Veterinaria
T1  - Energy-related hormones in raw and retail cow’s milk and possible risk for consumers
VL  - 74
IS  - 1
SP  - 1
EP  - 16
DO  - 10.2478/acve-2024-0001
ER  - 

@article{
author = "Knežević, Dragan and Bošnjaković, Dušan and Dražić, Slavica and Nedić, Sreten and Vujanac, Ivan and Valčić, Olivera and Pantelić, Marija and Stojiljković, Mojca and Sladojević, Željko and Kirovski, Danijela",
year = "2024",
abstract = "The study aimed to determine the concentration of energy-related hormones in cow’s
milk and to consider them from a public health perspective. Fourteen Holstein cows
were subjected to milk and blood sampling on the following days in lactation (DIL):
10, 30, 60, 90, 150, 180, 210, 250 and 280 to determine milk hormones, fat and protein
content and blood biochemical parameters. For the same purpose, bulk-tank milk
was sampled and samples of retail milk with 1.5% (CM1.5) and 3.2% (CM3.2) fat was
purchased. Milk insulin-like growth factor-1 (IGF-1) values were significantly lower at
90, 150, 180, 210 and 250 and significantly higher at 10, 30 and 60 DIL than lactation
average (LA). Milk insulin concentrations were significantly lower at 30, 60 and 90 and
higher at 210, 250 and 280 DIL than LA. Free thyroxine (fT4) level in the milk was
higher at 250 DIL, while milk free triiodothyronine (fT3) concentrations were lower
at 30, 60, 90 and 280 DIL, and significantly higher at 10 and 180 DIL than respective
LA. Milk cortisol levels were lower at 60 and 280 DIL than LA. All measured milk
hormones were significantly lower in CM1.5 compared to CM3.2, bulk-tank milk and
LA. An exception was the LA of IGF-1, which was significantly lower than the IGF-
1 content in CM1.5. Blood biochemical parameters fluctuated evenly during lactation
and were within the reference range. Hormone concentrations in cow’s milk fluctuate
during lactation, giving milk an important role in the context of public health.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Acta Veterinaria",
title = "Energy-related hormones in raw and retail cow’s milk and possible risk for consumers",
volume = "74",
number = "1",
pages = "1-16",
doi = "10.2478/acve-2024-0001"
}

Knežević, D., Bošnjaković, D., Dražić, S., Nedić, S., Vujanac, I., Valčić, O., Pantelić, M., Stojiljković, M., Sladojević, Ž.,& Kirovski, D.. (2024). Energy-related hormones in raw and retail cow’s milk and possible risk for consumers. in Acta Veterinaria
Beograd : Fakultet veterinarske medicine., 74(1), 1-16.
https://doi.org/10.2478/acve-2024-0001

Knežević D, Bošnjaković D, Dražić S, Nedić S, Vujanac I, Valčić O, Pantelić M, Stojiljković M, Sladojević Ž, Kirovski D. Energy-related hormones in raw and retail cow’s milk and possible risk for consumers. in Acta Veterinaria. 2024;74(1):1-16.
doi:10.2478/acve-2024-0001 .

Knežević, Dragan, Bošnjaković, Dušan, Dražić, Slavica, Nedić, Sreten, Vujanac, Ivan, Valčić, Olivera, Pantelić, Marija, Stojiljković, Mojca, Sladojević, Željko, Kirovski, Danijela, "Energy-related hormones in raw and retail cow’s milk and possible risk for consumers" in Acta Veterinaria, 74, no. 1 (2024):1-16,
https://doi.org/10.2478/acve-2024-0001 . .

TY  - CONF
AU  - Valčić, Olivera
AU  - Jovanović, Ivan B.
AU  - Milanović, Svetlana
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3085
AB  - Током раста бројлера долази до адаптивних промена антиоксидативног
статуса. Глутатион пероксидаза (GPx) је један од ензима који штите ћелије од
оксидативног стреса, а малондиалдехид (МДА) настаје током пероксидације
фослипида ћелијске мембране и поуздан је показатељ степена оксидативног
оштећења.
Циљ овог рада је био да се одреде промене активности GPx и концентрације
МДА током прве 4 недеље това (1., 14. и 28. дан), као и ефекат дијетарне
суплементације алфа-токоферолом (100 IU/kg хране).
Оглед је спроведен на товним пилићима старости од 1 дан до 28. дана това.
Пилад су била подељена у 2 огледне групе. Прва група тј. kонтрола (н=10) је
храњена стандардном смешом која задовољава све нутритивне потребе, док је
друга огледна група (н=10) добијала исту храну уз додатак 100 IU/kg хране
алфа-токоферола. У назначеним данима узимани су узорци хепаринисане
крви пункцијом крилне вене из које је у року од 2 часа издвојена крвна плазма,
у којој су одређивани GPx и МДА.
GPx мерена је куплованим тестом (Gunzler и сар. 1974). Принцип мерења је
базиран на спектрофотометријском регистровању утрошка NADPH у
куплованом ензимском систему. Малондиалдехид (МДА) је одређен методом
описаном од стране Андрееве, 1988.
Просечне вредности GPx су се кретале у интервалу од 21,46 до 38,55 µкат/л.
Најниже просечне вредности су забележене 14. дана това, док су највише
просечне вредности забележене 28. дана. Међутим, вредности активности GPx
се нису статистички разликовале (п>0,05), нити између контролне групе и
групе суплементиране алфа-токоферолом, нити приликом поређења група
током посматраног временског периода. Вредности МДА су биле најниже 14.
дана , при чему је контролна група имала сигнификантно (п<0,01) више
вредности МДА у односу на групу суплементирану алфа-токоферолом.
Значајан пораст концентрације МДА у плазми бројлера је забележен 28. дана
това, при чему су вредности концентрације у обе групе скоро утрострућене.
Несуплементирана група је имала сигнификантно веће вредности (п<0,05)
концентрације МДА у односу на суплементирану групу.
Резултати указују да током прве 4 недеље това долази до значајних промена
показатеља антиоксидативне заштите и нивоа пероксидације мембранских фосфолипида. Ефекат суплементираног алфа-токоферола је значајан са
аспекта смањења концентрације МДА у плазми.
AB  - In broilers during the growth stage, adaptive changes in antioxidant status occur.
Glutathione peroxidase (GPx) is one of the enzymes that protect cells from
oxidative stress, and malondialdehyde (MDA) is formed during the peroxidation
of cell membrane phospholipids and is a reliable indicator of the degree of
oxidative damage.
The aim of this work was to determine changes in GPx activity and MDA
concentration during the first 4 weeks of growth (1st, 14th and 28th day), as well
as the effects of dietary supplementation with alpha-tocopherol (100 IU/kg feed)
on the above parameters.
The experiment was conducted on broiler chickens aged from 1 day to 28 days of
fattening. The chicks were divided into 2 experimental groups. The first i.e.
control group (n=10) was fed a standard mixture that meets the needs, while the
second experimental group (n=10) received the same mixture with the addition
of 100 IU/kg of feed alpha-tocopherol. On the indicated days, samples of
heparinized blood were taken by puncture of the wing vein, from which blood
plasma was separated within 2 hours, in which GPx and MDA were determined.
GPx was measured by the coupled test (Gunzler et al. 1974). The test is based on
the spectrophotometric registration of NADPH consumption in the coupled
enzyme system. Malondialdehyde (MDA) was determined based on
spectrophotometric light absorption of the resulting stable pink solution, using
the method described by Andreeva, 1988.
The average values obtained for GPx ranged from 21.46 to 38.55 µkat/l. The lowest
average values were recorded on the 14th day of fattening, while the highest
average values were recorded on the 28th day. However, GPx activity values were
not statistically significantly different (p>0.05), neither between the control
group and the group supplemented with alpha-tocopherol, nor when comparing
the groups during the observed time period. MDA values were the lowest on day
14, where the control group had significantly (p<0.01) higher MDA values
compared to the group supplemented with alpha-tocopherol. A significant
increase in the concentration of MDA in broiler plasma was recorded on the 28th
day of fattening, where the concentration values in both groups were almost
tripled. The non-supplemented group had significantly higher values (p<0.05) of
MDA concentration compared to the supplemented group.
The results indicate that during the first 4 weeks of fattening, there are significant
changes in the indicators of antioxidant protection and the level of peroxidation of membrane phospholipids. The effect of supplemented alpha-tocopherol is
significant from the point of view of reducing the concentration of MDA in
plasma.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023
T1  - Uticaj alfa-tokoferola na aktivnost GPx i koncentraciju MDA u krvnoj plazmi brojlera
T1  - Influence of alpha-tocopherol on GPx activity and MDA concentration in the blood plasma of broilers
SP  - 179
EP  - 182
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3085
ER  - 

@conference{
author = "Valčić, Olivera and Jovanović, Ivan B. and Milanović, Svetlana",
year = "2023",
abstract = "Током раста бројлера долази до адаптивних промена антиоксидативног
статуса. Глутатион пероксидаза (GPx) је један од ензима који штите ћелије од
оксидативног стреса, а малондиалдехид (МДА) настаје током пероксидације
фослипида ћелијске мембране и поуздан је показатељ степена оксидативног
оштећења.
Циљ овог рада је био да се одреде промене активности GPx и концентрације
МДА током прве 4 недеље това (1., 14. и 28. дан), као и ефекат дијетарне
суплементације алфа-токоферолом (100 IU/kg хране).
Оглед је спроведен на товним пилићима старости од 1 дан до 28. дана това.
Пилад су била подељена у 2 огледне групе. Прва група тј. kонтрола (н=10) је
храњена стандардном смешом која задовољава све нутритивне потребе, док је
друга огледна група (н=10) добијала исту храну уз додатак 100 IU/kg хране
алфа-токоферола. У назначеним данима узимани су узорци хепаринисане
крви пункцијом крилне вене из које је у року од 2 часа издвојена крвна плазма,
у којој су одређивани GPx и МДА.
GPx мерена је куплованим тестом (Gunzler и сар. 1974). Принцип мерења је
базиран на спектрофотометријском регистровању утрошка NADPH у
куплованом ензимском систему. Малондиалдехид (МДА) је одређен методом
описаном од стране Андрееве, 1988.
Просечне вредности GPx су се кретале у интервалу од 21,46 до 38,55 µкат/л.
Најниже просечне вредности су забележене 14. дана това, док су највише
просечне вредности забележене 28. дана. Међутим, вредности активности GPx
се нису статистички разликовале (п>0,05), нити између контролне групе и
групе суплементиране алфа-токоферолом, нити приликом поређења група
током посматраног временског периода. Вредности МДА су биле најниже 14.
дана , при чему је контролна група имала сигнификантно (п<0,01) више
вредности МДА у односу на групу суплементирану алфа-токоферолом.
Значајан пораст концентрације МДА у плазми бројлера је забележен 28. дана
това, при чему су вредности концентрације у обе групе скоро утрострућене.
Несуплементирана група је имала сигнификантно веће вредности (п<0,05)
концентрације МДА у односу на суплементирану групу.
Резултати указују да током прве 4 недеље това долази до значајних промена
показатеља антиоксидативне заштите и нивоа пероксидације мембранских фосфолипида. Ефекат суплементираног алфа-токоферола је значајан са
аспекта смањења концентрације МДА у плазми., In broilers during the growth stage, adaptive changes in antioxidant status occur.
Glutathione peroxidase (GPx) is one of the enzymes that protect cells from
oxidative stress, and malondialdehyde (MDA) is formed during the peroxidation
of cell membrane phospholipids and is a reliable indicator of the degree of
oxidative damage.
The aim of this work was to determine changes in GPx activity and MDA
concentration during the first 4 weeks of growth (1st, 14th and 28th day), as well
as the effects of dietary supplementation with alpha-tocopherol (100 IU/kg feed)
on the above parameters.
The experiment was conducted on broiler chickens aged from 1 day to 28 days of
fattening. The chicks were divided into 2 experimental groups. The first i.e.
control group (n=10) was fed a standard mixture that meets the needs, while the
second experimental group (n=10) received the same mixture with the addition
of 100 IU/kg of feed alpha-tocopherol. On the indicated days, samples of
heparinized blood were taken by puncture of the wing vein, from which blood
plasma was separated within 2 hours, in which GPx and MDA were determined.
GPx was measured by the coupled test (Gunzler et al. 1974). The test is based on
the spectrophotometric registration of NADPH consumption in the coupled
enzyme system. Malondialdehyde (MDA) was determined based on
spectrophotometric light absorption of the resulting stable pink solution, using
the method described by Andreeva, 1988.
The average values obtained for GPx ranged from 21.46 to 38.55 µkat/l. The lowest
average values were recorded on the 14th day of fattening, while the highest
average values were recorded on the 28th day. However, GPx activity values were
not statistically significantly different (p>0.05), neither between the control
group and the group supplemented with alpha-tocopherol, nor when comparing
the groups during the observed time period. MDA values were the lowest on day
14, where the control group had significantly (p<0.01) higher MDA values
compared to the group supplemented with alpha-tocopherol. A significant
increase in the concentration of MDA in broiler plasma was recorded on the 28th
day of fattening, where the concentration values in both groups were almost
tripled. The non-supplemented group had significantly higher values (p<0.05) of
MDA concentration compared to the supplemented group.
The results indicate that during the first 4 weeks of fattening, there are significant
changes in the indicators of antioxidant protection and the level of peroxidation of membrane phospholipids. The effect of supplemented alpha-tocopherol is
significant from the point of view of reducing the concentration of MDA in
plasma.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023",
title = "Uticaj alfa-tokoferola na aktivnost GPx i koncentraciju MDA u krvnoj plazmi brojlera, Influence of alpha-tocopherol on GPx activity and MDA concentration in the blood plasma of broilers",
pages = "179-182",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3085"
}

Valčić, O., Jovanović, I. B.,& Milanović, S.. (2023). Uticaj alfa-tokoferola na aktivnost GPx i koncentraciju MDA u krvnoj plazmi brojlera. in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023
Banja Luka : Veterinarska komora Republike Srpske., 179-182.
https://hdl.handle.net/21.15107/rcub_veterinar_3085

Valčić O, Jovanović IB, Milanović S. Uticaj alfa-tokoferola na aktivnost GPx i koncentraciju MDA u krvnoj plazmi brojlera. in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023. 2023;:179-182.
https://hdl.handle.net/21.15107/rcub_veterinar_3085 .

Valčić, Olivera, Jovanović, Ivan B., Milanović, Svetlana, "Uticaj alfa-tokoferola na aktivnost GPx i koncentraciju MDA u krvnoj plazmi brojlera" in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023 (2023):179-182,
https://hdl.handle.net/21.15107/rcub_veterinar_3085 .

TY  - CONF
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
AU  - Jovanović, Ivan B.
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3107
AB  - Gastrointestinalni trakt predstavlja kompleksan ekosistem koji sadrži veliki broj
metabolički aktivnih mikroorganizama. Među značajnim metabolitima mikrobioma posebno se
ističu masne kiseline kratkih lanaca (SCFA), sekundarne žučne kiseline, poliamini i indol, zajedno
sa neurotransmiterima kao što su gama-aminobutirat, serotonin i kateholamini. Brojni radovi
dokazuju uticaj metabolita mikrobioma na rast, prirast, stres i imunske reakcije organizma.
Međutim, tek u novijim radovima se ukazuje na činjenicu da ne utiče samo mikrobiom svojim
metabolitima na zdrastveni status svinja, već da postoje znatno složenije interakcije između
domaćina i mikrobioma. Ovom prilikom, želimo da istaknemo ne samo već poznat značaj
mikrobioma za zdravlje i performanse svinja, već i najnovija naučna saznanja iz oblasti složenih
interakcija mikrobiom-domaćin (svinja)- mikrobiom- crevno/nervna osovina (gut brain axis).
Cilj ovog rada je da na slikovit i razumljiv način objasni najnovija saznanja iz oblasti
složenih metaboličkih interakcija koje postoje između mikrobioma, hrane, crevno-moždane
osovine i domaćina kako bi se implementirala u intenzivnom farmskom uzgoju svinja u cilju
poboljšanja dobrobiti i zdravlja životinja.
PB  - Požarevac : Sitograf RM
C3  - 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023
T1  - Značaj i interakcije mikrobioma gastrointestinalnog trakta i metabolizma svinja
SP  - 158
EP  - 164
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3107
ER  - 

@conference{
author = "Valčić, Olivera and Milanović, Svetlana and Jovanović, Ivan B.",
year = "2023",
abstract = "Gastrointestinalni trakt predstavlja kompleksan ekosistem koji sadrži veliki broj
metabolički aktivnih mikroorganizama. Među značajnim metabolitima mikrobioma posebno se
ističu masne kiseline kratkih lanaca (SCFA), sekundarne žučne kiseline, poliamini i indol, zajedno
sa neurotransmiterima kao što su gama-aminobutirat, serotonin i kateholamini. Brojni radovi
dokazuju uticaj metabolita mikrobioma na rast, prirast, stres i imunske reakcije organizma.
Međutim, tek u novijim radovima se ukazuje na činjenicu da ne utiče samo mikrobiom svojim
metabolitima na zdrastveni status svinja, već da postoje znatno složenije interakcije između
domaćina i mikrobioma. Ovom prilikom, želimo da istaknemo ne samo već poznat značaj
mikrobioma za zdravlje i performanse svinja, već i najnovija naučna saznanja iz oblasti složenih
interakcija mikrobiom-domaćin (svinja)- mikrobiom- crevno/nervna osovina (gut brain axis).
Cilj ovog rada je da na slikovit i razumljiv način objasni najnovija saznanja iz oblasti
složenih metaboličkih interakcija koje postoje između mikrobioma, hrane, crevno-moždane
osovine i domaćina kako bi se implementirala u intenzivnom farmskom uzgoju svinja u cilju
poboljšanja dobrobiti i zdravlja životinja.",
publisher = "Požarevac : Sitograf RM",
journal = "20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023",
title = "Značaj i interakcije mikrobioma gastrointestinalnog trakta i metabolizma svinja",
pages = "158-164",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3107"
}

Valčić, O., Milanović, S.,& Jovanović, I. B.. (2023). Značaj i interakcije mikrobioma gastrointestinalnog trakta i metabolizma svinja. in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023
Požarevac : Sitograf RM., 158-164.
https://hdl.handle.net/21.15107/rcub_veterinar_3107

Valčić O, Milanović S, Jovanović IB. Značaj i interakcije mikrobioma gastrointestinalnog trakta i metabolizma svinja. in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023. 2023;:158-164.
https://hdl.handle.net/21.15107/rcub_veterinar_3107 .

Valčić, Olivera, Milanović, Svetlana, Jovanović, Ivan B., "Značaj i interakcije mikrobioma gastrointestinalnog trakta i metabolizma svinja" in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023 (2023):158-164,
https://hdl.handle.net/21.15107/rcub_veterinar_3107 .

TY  - CONF
AU  - Valčić, Miroslav
AU  - Valčić, Olivera
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3070
AB  - Појам „серолошке реакције“, односи се на методе дијагностике пре свега
заразних болести животиња (и људи) у којима се користе:
а. серуми потенцијално болесних пацијената у којима се доказују присутна
антитела и
б. претходно припремљени специфични серуми за доказивање антигена у
узорцима пореклом од пацијената.
Међутим, у оба случаја се у серуму налази велики број имуноглобулина који,
иако су сви специфични за дати узрочник обољења, ипак поседују различите
специфичнисти тј. паратопе, за поједине епитопе којих је у структури
узрочника, велики број. То значи да је такав поликлонски серум, настао као
последица имунизације целим микроорганизмом.
Моноклонска антитела производе хибридне ћелије (хибридоми), настале
спајањем спленоцита који синтетишу имуноглобулин (Б ћелије) и
мијеломских ћелија. Таква хибридна ћелија, специфичност дугује спленоциту
тј. имунској ћелији слезине имунизованог на пример миша, а бесмртност је
последица генетског материјала туморске, мијеломске ћелије. Револуција у
серологији коју је ова техника изазвала, последица је чињенице да таква ћелија
неограничено лучи антитела специфична за појединачни епитоп у структури
микроорганизма. Када знамо да микроорганизими (вируси, бактерије)
поседују веома велики број различитих епитопа (структурни, површински,
производи метаболизма...), који код великог броја микроба значајно варирају,
онда је јасно да се моноклонским антителима могу детектовати практично све
варијације у антигеној структури на пример вируса, а као последица мутација
(вирус корона) или реасортирања (ортомиксовирус).
Jедан од основних принципа серолошких реакција јесте да се комплекс
антиген-антитело учини видљивим. Ензимске имуно реакције (ЕЛИСА) већ
дужи период времена предстаљају значајан сегмент серолошке дијагностике
заразних болести (леукоза говеда на пример). Заснивају се на видљивој
реакцији која је последица разлагања супстрата под дејством ензима који је
везан за молекул антитела или (ређе) антигена. Читање резултата је
објективно, спектрофотометром.
Моноклонска антитела и ЕЛИСА представља комбинацију у серолошким
дијагностичким испитивањима, која са једне стране може да детектује антиген
тј. узрочник заразне болести и његове варијације али и да, применом „сендвич“
методе, докаже сероконверзију оболеле животиње. У раду ће бити приказани основни принципи наведених метода као и њихова
примена у серолошкој дијагностици заразних болести животиња.
AB  - The term "serological reactions" refers to methods of diagnosis primarily
of infectious diseases of animals (and humans) in which they use as follows:
a. sera of potentially sick patients in which the presence of antibodies is proven and
b. previously prepared specific sera for proving antigens in patient samples.
However, in both cases, there is a large number of immunoglobulins in the serum,
which, although they are all specific for the given causative diseases, still have
different specificities, ie. paratopes, for certain epitopes of which there are a large
number in the structure of the causative agent. This means that such a polyclonal
serum was created as a result of immunization with the whole microorganism.
Monoclonal antibodies are produced by hybrid cells (hybridomas), formed by the
fusion of immunoglobulin-synthesizing splenocytes (B cells) and myeloma cells. Such
a hybrid cell owes its specificity to the splenocyte, ie the immune cell of the spleen of
an immunized mouse, for example, and its immortality is a consequence of the genetic
material of a tumor, myeloma cell. The revolution in serology caused by this technique
is a consequence of the fact that a given cell indefinitely secretes antibodies specific
for an individual epitope in the microorganism's structure. Bearing in mind that
microorganisms (viruses, bacteria) possess a very large number of different epitopes
(structural, surface, metabolic products...), which vary significantly in a large number
of microbes, then it is clear that monoclonal antibodies can detect practically all
variations in the antigenic structure for example viruses, and as a result of mutations
(corona virus) or reassortment (orthomyxovirus).
One of the basic principles of serological reactions is to make the antigen-antibody
complex visible. Enzyme immuno assays (ELISA) have been a significant segment of
serological diagnostics of infectious diseases (bovine leukosis, for example) for a long
time. They are based on a visible reaction that is a consequence of the decomposition
of the substrate under the action of an enzyme that is bound to an antibody molecule
or (rarely) an antigen. The reading of the results is objective, with a
spectrophotometer.
Monoclonal antibodies and ELISA represent a combination in serological diagnostic
tests, which, on the one hand, can detect the antigen, i.e. the causative agent of an
infectious disease and its variations, but also to prove the seroconversion of an
infected animal, by the so called "sandwich" method.
The paper will present the basic principles of the mentioned methods as well as their
application in the serological diagnosis of infectious animal diseases.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023
T1  - Monoklonska antitela + elisa - dobitna kombinacija u dijagnostici zaraznih bolesti životinja
T1  - Monoclonal antibodies + elisa - a winning combination in the diagnosis of infectious diseases of animals
VL  - 41
EP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3070
ER  - 

@conference{
author = "Valčić, Miroslav and Valčić, Olivera",
year = "2023",
abstract = "Појам „серолошке реакције“, односи се на методе дијагностике пре свега
заразних болести животиња (и људи) у којима се користе:
а. серуми потенцијално болесних пацијената у којима се доказују присутна
антитела и
б. претходно припремљени специфични серуми за доказивање антигена у
узорцима пореклом од пацијената.
Међутим, у оба случаја се у серуму налази велики број имуноглобулина који,
иако су сви специфични за дати узрочник обољења, ипак поседују различите
специфичнисти тј. паратопе, за поједине епитопе којих је у структури
узрочника, велики број. То значи да је такав поликлонски серум, настао као
последица имунизације целим микроорганизмом.
Моноклонска антитела производе хибридне ћелије (хибридоми), настале
спајањем спленоцита који синтетишу имуноглобулин (Б ћелије) и
мијеломских ћелија. Таква хибридна ћелија, специфичност дугује спленоциту
тј. имунској ћелији слезине имунизованог на пример миша, а бесмртност је
последица генетског материјала туморске, мијеломске ћелије. Револуција у
серологији коју је ова техника изазвала, последица је чињенице да таква ћелија
неограничено лучи антитела специфична за појединачни епитоп у структури
микроорганизма. Када знамо да микроорганизими (вируси, бактерије)
поседују веома велики број различитих епитопа (структурни, површински,
производи метаболизма...), који код великог броја микроба значајно варирају,
онда је јасно да се моноклонским антителима могу детектовати практично све
варијације у антигеној структури на пример вируса, а као последица мутација
(вирус корона) или реасортирања (ортомиксовирус).
Jедан од основних принципа серолошких реакција јесте да се комплекс
антиген-антитело учини видљивим. Ензимске имуно реакције (ЕЛИСА) већ
дужи период времена предстаљају значајан сегмент серолошке дијагностике
заразних болести (леукоза говеда на пример). Заснивају се на видљивој
реакцији која је последица разлагања супстрата под дејством ензима који је
везан за молекул антитела или (ређе) антигена. Читање резултата је
објективно, спектрофотометром.
Моноклонска антитела и ЕЛИСА представља комбинацију у серолошким
дијагностичким испитивањима, која са једне стране може да детектује антиген
тј. узрочник заразне болести и његове варијације али и да, применом „сендвич“
методе, докаже сероконверзију оболеле животиње. У раду ће бити приказани основни принципи наведених метода као и њихова
примена у серолошкој дијагностици заразних болести животиња., The term "serological reactions" refers to methods of diagnosis primarily
of infectious diseases of animals (and humans) in which they use as follows:
a. sera of potentially sick patients in which the presence of antibodies is proven and
b. previously prepared specific sera for proving antigens in patient samples.
However, in both cases, there is a large number of immunoglobulins in the serum,
which, although they are all specific for the given causative diseases, still have
different specificities, ie. paratopes, for certain epitopes of which there are a large
number in the structure of the causative agent. This means that such a polyclonal
serum was created as a result of immunization with the whole microorganism.
Monoclonal antibodies are produced by hybrid cells (hybridomas), formed by the
fusion of immunoglobulin-synthesizing splenocytes (B cells) and myeloma cells. Such
a hybrid cell owes its specificity to the splenocyte, ie the immune cell of the spleen of
an immunized mouse, for example, and its immortality is a consequence of the genetic
material of a tumor, myeloma cell. The revolution in serology caused by this technique
is a consequence of the fact that a given cell indefinitely secretes antibodies specific
for an individual epitope in the microorganism's structure. Bearing in mind that
microorganisms (viruses, bacteria) possess a very large number of different epitopes
(structural, surface, metabolic products...), which vary significantly in a large number
of microbes, then it is clear that monoclonal antibodies can detect practically all
variations in the antigenic structure for example viruses, and as a result of mutations
(corona virus) or reassortment (orthomyxovirus).
One of the basic principles of serological reactions is to make the antigen-antibody
complex visible. Enzyme immuno assays (ELISA) have been a significant segment of
serological diagnostics of infectious diseases (bovine leukosis, for example) for a long
time. They are based on a visible reaction that is a consequence of the decomposition
of the substrate under the action of an enzyme that is bound to an antibody molecule
or (rarely) an antigen. The reading of the results is objective, with a
spectrophotometer.
Monoclonal antibodies and ELISA represent a combination in serological diagnostic
tests, which, on the one hand, can detect the antigen, i.e. the causative agent of an
infectious disease and its variations, but also to prove the seroconversion of an
infected animal, by the so called "sandwich" method.
The paper will present the basic principles of the mentioned methods as well as their
application in the serological diagnosis of infectious animal diseases.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023",
title = "Monoklonska antitela + elisa - dobitna kombinacija u dijagnostici zaraznih bolesti životinja, Monoclonal antibodies + elisa - a winning combination in the diagnosis of infectious diseases of animals",
volume = "41",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3070"
}

Valčić, M.,& Valčić, O.. (2023). Monoklonska antitela + elisa - dobitna kombinacija u dijagnostici zaraznih bolesti životinja. in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023
Banja Luka : Veterinarska komora Republike Srpske., 41.
https://hdl.handle.net/21.15107/rcub_veterinar_3070

Valčić M, Valčić O. Monoklonska antitela + elisa - dobitna kombinacija u dijagnostici zaraznih bolesti životinja. in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023. 2023;41:null-43.
https://hdl.handle.net/21.15107/rcub_veterinar_3070 .

Valčić, Miroslav, Valčić, Olivera, "Monoklonska antitela + elisa - dobitna kombinacija u dijagnostici zaraznih bolesti životinja" in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023, 41 (2023),
https://hdl.handle.net/21.15107/rcub_veterinar_3070 .

TY  - CONF
AU  - Milanović, Svetlana
AU  - Valčić, Olivera
AU  - Gvozdić, Dragan
AU  - Jovanović, Ivan B.
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2418
AB  - U intenzivnoj stočarskoj proizvodnji, od muznih krava se očekuje maksimalno iskorišćavanje genetskog potencijala i što veći prinos
mleka. Najveći izazov u tom smislu predstavlja peripartalni period koji obuhvata tri nedelje pre i tri nedelje posle partusa. Tada se u organizmu krave dešavaju velike hormonske, metaboličke i imunološke
promene. Mnogi metabolički poremećaji koji su karakteristični za ovaj
period, posledica su neizbalansirane ishrane i nepravilne pripreme jedinke za ulazak u intenzivnu laktaciju. Masna jetra, ketoza, dislokacija
sirišta i puerperalna pareza predstavljaju glavne poremećaje karakteristične za ovaj period, a oksidativni stres može imati veliku ulogu u
njihovoj pojavi. Osim navedenih poremećaja, zaostajanje posteljice i
pojava mastitisa, mogu takođe biti u vezi sa oksidativnim stresom. U
ovom radu ćemo analizirati podatke iz naših istraživanja i dostupne literature i pokušati da objasnimo ulogu oksidativnog stresa u peripartalnom periodu, sa posebnim osvrtom na primenu antioksidanasa u cilju preveniranja pojave ili posledica metaboličkih poremećaja.
AB  - In intensive production, dairy cows are expected to make the most of their genetic
potential and maximize milk yield. The biggest challenge in this regard is the peripartal
period, which includes three weeks before and three weeks after parturition. In that interval
major hormonal, metabolic and immune changes occur in the cow’s body. Many metabolic
disorders that are characteristic of this period are the result of an unbalanced diet and
improper preparation of the individual to enter intensive lactation. Fatty liver, ketosis,
dislocation of the abomasum, and puerperal paresis are the main disorders which are
characteristic of this period, and oxidative stress can play a major role in their occurrence.
In addition to these disorders, retained placenta and mastitis may also be related to
oxidative stress. In this paper, we will analyze the data from our research and the available literature, and try to explain the role of oxidative stress in the peripartal period, with special
emphasis on the use of antioxidants in the prevention of the occurrence or consequences
of metabolic disorders.
PB  - Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - Zbornik radova - XLIII seminar inovacija znanja veterinara
T1  - Oksidativni stres kod krava u peripartalnom periodu – uzrok ili posledica metaboličkih poremećaja
T1  - Oxidative stress in cows in the peripartal period – cause or consequence of metabolic disorders
SP  - 67
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2418
ER  - 

@conference{
author = "Milanović, Svetlana and Valčić, Olivera and Gvozdić, Dragan and Jovanović, Ivan B.",
year = "2022",
abstract = "U intenzivnoj stočarskoj proizvodnji, od muznih krava se očekuje maksimalno iskorišćavanje genetskog potencijala i što veći prinos
mleka. Najveći izazov u tom smislu predstavlja peripartalni period koji obuhvata tri nedelje pre i tri nedelje posle partusa. Tada se u organizmu krave dešavaju velike hormonske, metaboličke i imunološke
promene. Mnogi metabolički poremećaji koji su karakteristični za ovaj
period, posledica su neizbalansirane ishrane i nepravilne pripreme jedinke za ulazak u intenzivnu laktaciju. Masna jetra, ketoza, dislokacija
sirišta i puerperalna pareza predstavljaju glavne poremećaje karakteristične za ovaj period, a oksidativni stres može imati veliku ulogu u
njihovoj pojavi. Osim navedenih poremećaja, zaostajanje posteljice i
pojava mastitisa, mogu takođe biti u vezi sa oksidativnim stresom. U
ovom radu ćemo analizirati podatke iz naših istraživanja i dostupne literature i pokušati da objasnimo ulogu oksidativnog stresa u peripartalnom periodu, sa posebnim osvrtom na primenu antioksidanasa u cilju preveniranja pojave ili posledica metaboličkih poremećaja., In intensive production, dairy cows are expected to make the most of their genetic
potential and maximize milk yield. The biggest challenge in this regard is the peripartal
period, which includes three weeks before and three weeks after parturition. In that interval
major hormonal, metabolic and immune changes occur in the cow’s body. Many metabolic
disorders that are characteristic of this period are the result of an unbalanced diet and
improper preparation of the individual to enter intensive lactation. Fatty liver, ketosis,
dislocation of the abomasum, and puerperal paresis are the main disorders which are
characteristic of this period, and oxidative stress can play a major role in their occurrence.
In addition to these disorders, retained placenta and mastitis may also be related to
oxidative stress. In this paper, we will analyze the data from our research and the available literature, and try to explain the role of oxidative stress in the peripartal period, with special
emphasis on the use of antioxidants in the prevention of the occurrence or consequences
of metabolic disorders.",
publisher = "Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "Zbornik radova - XLIII seminar inovacija znanja veterinara",
title = "Oksidativni stres kod krava u peripartalnom periodu – uzrok ili posledica metaboličkih poremećaja, Oxidative stress in cows in the peripartal period – cause or consequence of metabolic disorders",
pages = "67-78",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2418"
}

Milanović, S., Valčić, O., Gvozdić, D.,& Jovanović, I. B.. (2022). Oksidativni stres kod krava u peripartalnom periodu – uzrok ili posledica metaboličkih poremećaja. in Zbornik radova - XLIII seminar inovacija znanja veterinara
Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 67-78.
https://hdl.handle.net/21.15107/rcub_veterinar_2418

Milanović S, Valčić O, Gvozdić D, Jovanović IB. Oksidativni stres kod krava u peripartalnom periodu – uzrok ili posledica metaboličkih poremećaja. in Zbornik radova - XLIII seminar inovacija znanja veterinara. 2022;:67-78.
https://hdl.handle.net/21.15107/rcub_veterinar_2418 .

Milanović, Svetlana, Valčić, Olivera, Gvozdić, Dragan, Jovanović, Ivan B., "Oksidativni stres kod krava u peripartalnom periodu – uzrok ili posledica metaboličkih poremećaja" in Zbornik radova - XLIII seminar inovacija znanja veterinara (2022):67-78,
https://hdl.handle.net/21.15107/rcub_veterinar_2418 .

TY  - JOUR
AU  - Valčić, Olivera
AU  - Milosavljević, Petar
AU  - Jovanović, Ivan B.
AU  - Milanović, Svetlana
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3373
AB  - Глутатион пероксидаза (GPx) је ензим који постоји у 8 изоензимских
облика, од којих GPx-1 је облигаторни интрацелуларни ензим, док је GPx-3
активна у екстрацелуларним течностима, посебно у крвној плазми. Основна
улога GPx се огледа у заштити ћелија од оксидативног стреса којег индукују
слободни кисеонични радикали. Активност GPx се узима као поуздан
индикатор статуса селена у организму људи и животиња. Основни пут уноса
селена у организам је преко ланца исхране. Унети селен се уграђује у облику
селенометионина и селеноцистеина у ткивне протеине, односно ензиме. У
Србији су извршена темељна испитивања садржаја селена у хранивима и
домаћим животињама, посебно код економски значајних врста као што су
живина, свиње, овце и говеда. Међутим, до сада нису детаљно испитани
радни хладнокрвни коњи, посебно они који се хране искључиво локално
узгајаним хранивима, или су на испаши. Услед специфичног начина држања
они су идеални индикатори статуса селена моногастричних биљоједа на
датом локалитету.
Циљ нашег истраживања је био да одредимо статус селена на основу
активности GPx-1 и GPx -3 у узорцима крви несуплементираних радних
коња на територији централне Србије.
У нашем истраживању укупно је испитано 12 узорака крви коња преузетих
са локалитета општина Краљево, Зајечар, Ваљево и Димитровград, и то: 12
узорака крвне плазме и 12 узорака испраних еритроцита. Мерење
активности GPx-3 и GPx-1 је вршено методом по Гунцлеру на таласној
дужини од 366nm. Приликом узорковања за свако грло су евидентирани
подаци о полу, старости, вакцинацији и дехелминтизацији, саставу и
пореклу датих хранива. Просечна активност GPx-1 је 502,02 ± 91,77 μKat/l , а GPx-3 3,46 ± 1,02
μKat/l што указује на постојање маргиналног дефицита селена у популацији
несуплементираних радних коња на територији централне Србије.
AB  - Glutathione peroxidase (GPx) is an enzyme that has 8 isoenzyme forms, of which
GPx-1 is an obligatory intracellular enzyme, while GPx-3 is active in extracellular
fluids, especially in blood plasma. The main role of GPx is the protection of cells
from oxidative stress induced by free oxygen radicals. GPx activity is taken as a
reliable indicator of selenium status in the human and animal organism. Selenium
is introduced in organism mainly through the food chain. Ingested selenium is
incorporated in the form of selenomethionine and selenocysteine into tissue
proteins, i.e. enzymes. Detailed investigation related to the content of selenium in
feed and domestic animals were carried out in Serbia, especially in economically
significant species such as poultry, pigs, sheep and cattle. However, working coldblooded
horses, especially those that are fed exclusively with locally grown feed,
or are on pasture, have not been examined in detail so far. Due to their specific
way of breeding, they are ideal indicators of the selenium status of monogastric
herbivores in a given locality.
The goal of our study was to determine the status of selenium based on the
activity of GPx-1 and GPx-3 in blood samples of non-supplemented working
horses in the territory of central Serbia.
In our study, a total of 12 samples of horse blood taken from the localities of the
municipalities of Kraljevo, Zaječar, Valjevo and Dimitrovgrad were tested as
follows: 12 samples of blood plasma and 12 samples of washed erythrocytes.
Measurement of GPx-3 and GPx-1 activity was carried out using the Guncler
method at a wavelength of 366 nm. For each animal, during sampling, data on
gender, age, vaccination and deworming, composition and origin of the given
nutrients were recorded. The average activity of GPx-1 was 502.02 ± 91.77 μKat/l, and GPx-3 3.46 ± 1.02
μKat/l, which indicates the existence of a marginal selenium deficit in the
population of unsupplemented working horses in the territory of central Serbia.
PB  - Banja Luka : PI Veterinary institute of Republic of Srpska 'Dr Vaso Butozan'
T2  - Veterinary Journal of Republic of Srpska
T1  - Aktivnost selenoenzima GPx-1 i GPx-3 u krvi radnih konja na teritoriji centralne Srbije
T1  - Activity of selenenzymes GPx-1 and GPx-3 in the blood of working horses in the territory of central Serbia
VL  - 22
IS  - 1-2
SP  - 178
EP  - 193
DO  - 10.7251/VETJSR2201178V
DO  - 10.7251/VETJEN2201186V
ER  - 

@article{
author = "Valčić, Olivera and Milosavljević, Petar and Jovanović, Ivan B. and Milanović, Svetlana",
year = "2022",
abstract = "Глутатион пероксидаза (GPx) је ензим који постоји у 8 изоензимских
облика, од којих GPx-1 је облигаторни интрацелуларни ензим, док је GPx-3
активна у екстрацелуларним течностима, посебно у крвној плазми. Основна
улога GPx се огледа у заштити ћелија од оксидативног стреса којег индукују
слободни кисеонични радикали. Активност GPx се узима као поуздан
индикатор статуса селена у организму људи и животиња. Основни пут уноса
селена у организам је преко ланца исхране. Унети селен се уграђује у облику
селенометионина и селеноцистеина у ткивне протеине, односно ензиме. У
Србији су извршена темељна испитивања садржаја селена у хранивима и
домаћим животињама, посебно код економски значајних врста као што су
живина, свиње, овце и говеда. Међутим, до сада нису детаљно испитани
радни хладнокрвни коњи, посебно они који се хране искључиво локално
узгајаним хранивима, или су на испаши. Услед специфичног начина држања
они су идеални индикатори статуса селена моногастричних биљоједа на
датом локалитету.
Циљ нашег истраживања је био да одредимо статус селена на основу
активности GPx-1 и GPx -3 у узорцима крви несуплементираних радних
коња на територији централне Србије.
У нашем истраживању укупно је испитано 12 узорака крви коња преузетих
са локалитета општина Краљево, Зајечар, Ваљево и Димитровград, и то: 12
узорака крвне плазме и 12 узорака испраних еритроцита. Мерење
активности GPx-3 и GPx-1 је вршено методом по Гунцлеру на таласној
дужини од 366nm. Приликом узорковања за свако грло су евидентирани
подаци о полу, старости, вакцинацији и дехелминтизацији, саставу и
пореклу датих хранива. Просечна активност GPx-1 је 502,02 ± 91,77 μKat/l , а GPx-3 3,46 ± 1,02
μKat/l што указује на постојање маргиналног дефицита селена у популацији
несуплементираних радних коња на територији централне Србије., Glutathione peroxidase (GPx) is an enzyme that has 8 isoenzyme forms, of which
GPx-1 is an obligatory intracellular enzyme, while GPx-3 is active in extracellular
fluids, especially in blood plasma. The main role of GPx is the protection of cells
from oxidative stress induced by free oxygen radicals. GPx activity is taken as a
reliable indicator of selenium status in the human and animal organism. Selenium
is introduced in organism mainly through the food chain. Ingested selenium is
incorporated in the form of selenomethionine and selenocysteine into tissue
proteins, i.e. enzymes. Detailed investigation related to the content of selenium in
feed and domestic animals were carried out in Serbia, especially in economically
significant species such as poultry, pigs, sheep and cattle. However, working coldblooded
horses, especially those that are fed exclusively with locally grown feed,
or are on pasture, have not been examined in detail so far. Due to their specific
way of breeding, they are ideal indicators of the selenium status of monogastric
herbivores in a given locality.
The goal of our study was to determine the status of selenium based on the
activity of GPx-1 and GPx-3 in blood samples of non-supplemented working
horses in the territory of central Serbia.
In our study, a total of 12 samples of horse blood taken from the localities of the
municipalities of Kraljevo, Zaječar, Valjevo and Dimitrovgrad were tested as
follows: 12 samples of blood plasma and 12 samples of washed erythrocytes.
Measurement of GPx-3 and GPx-1 activity was carried out using the Guncler
method at a wavelength of 366 nm. For each animal, during sampling, data on
gender, age, vaccination and deworming, composition and origin of the given
nutrients were recorded. The average activity of GPx-1 was 502.02 ± 91.77 μKat/l, and GPx-3 3.46 ± 1.02
μKat/l, which indicates the existence of a marginal selenium deficit in the
population of unsupplemented working horses in the territory of central Serbia.",
publisher = "Banja Luka : PI Veterinary institute of Republic of Srpska 'Dr Vaso Butozan'",
journal = "Veterinary Journal of Republic of Srpska",
title = "Aktivnost selenoenzima GPx-1 i GPx-3 u krvi radnih konja na teritoriji centralne Srbije, Activity of selenenzymes GPx-1 and GPx-3 in the blood of working horses in the territory of central Serbia",
volume = "22",
number = "1-2",
pages = "178-193",
doi = "10.7251/VETJSR2201178V, 10.7251/VETJEN2201186V"
}

Valčić, O., Milosavljević, P., Jovanović, I. B.,& Milanović, S.. (2022). Aktivnost selenoenzima GPx-1 i GPx-3 u krvi radnih konja na teritoriji centralne Srbije. in Veterinary Journal of Republic of Srpska
Banja Luka : PI Veterinary institute of Republic of Srpska 'Dr Vaso Butozan'., 22(1-2), 178-193.
https://doi.org/10.7251/VETJSR2201178V

Valčić O, Milosavljević P, Jovanović IB, Milanović S. Aktivnost selenoenzima GPx-1 i GPx-3 u krvi radnih konja na teritoriji centralne Srbije. in Veterinary Journal of Republic of Srpska. 2022;22(1-2):178-193.
doi:10.7251/VETJSR2201178V .

Valčić, Olivera, Milosavljević, Petar, Jovanović, Ivan B., Milanović, Svetlana, "Aktivnost selenoenzima GPx-1 i GPx-3 u krvi radnih konja na teritoriji centralne Srbije" in Veterinary Journal of Republic of Srpska, 22, no. 1-2 (2022):178-193,
https://doi.org/10.7251/VETJSR2201178V . .

TY  - JOUR
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
AU  - Došenović, Milan
AU  - Özvegy, József
AU  - Radaković, Milena
AU  - Vejnović, Branislav
AU  - Vučićević, Miloš
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2215
AB  - Introduction. Glutathione peroxidases are selenoenzymes which have a crucial role in the protection of animals against oxidative stress. 
Materials and Methods. From September 2017 to April 2018, a group of eight red-eared sliders were admitted at the Clinic for Small Animals, Faculty of Veterinary Medicine, University of Belgrade for elective diagnostic celioscopy. The turtles were of unknown age, weight from 1.20 kg to 1.86 kg. The anesthesia protocol involved using ketamine and medetomidine, both at a low dosage (10 mg kg-1 and 0.1 mg kg-1, respectively) as induction, after which anesthesia was maintained using isoflurane at 3% (vapor setting) in 100% oxygen (0.5 L min-1). Medetomidine was reversed with atipamezole (0.2 mg kg-1), given intramuscularly. The elective celioscopy was performed according to standard protocols. One day prior to anesthesia, heparinized blood samples were taken using the subcarapacial venous plexus for venipuncture. The second sampling took place three hours after the anesthetics were administered. 
Results and Conclusions. GPx3 activity in the blood plasmas (n=8) was measured by the coupled test as described by Günzler et al. (1974). Data were tested for normality by the Shapiro-Wilk normality test and the groups were compared using a paired t-test. 
Blood plasma GPx3 activity was significantly higher (p=0.009) after a three-hour recovery period from inhalation anesthesia performed for elective diagnostic celioscopy, than before anesthesia. The measured post-anesthesia GPx3 activities were, on average, 
80% higher than the measurements prior to anesthesia. It can be concluded that the statistically significant increase in the activity of plasma GPx3 from 91.02±36.05 mKat L-1 prior to anesthesia to 160.21±58.94 mKat L-1 three hours after anesthesia is due to the change in oxygen saturation. This is increased to 100% during the procedure, thus exposing the turtles to conditions of high oxygen saturation.
AB  - Uvod. Glutation peroksidaze su selenoenzimi koji igraju ključnu ulogu u zaštiti životinja od oksidativnog stresa. 
Materijal i metode. Od septembra 2017. do aprila 2018. godine, grupa od osam crvenouhih kornjača je primljena na Kliniku za male životinje, Fakulteta Veterinarske Medicine, Univerziteta u Beogradu zbog izvođenja elektivne dijagnostičke celioskopije. Kornjače su bile nepoznate starosti, mase između 1,20 kg i 1,86 kg. Anastetički protokol je uključivao primenu ketamina (10 mg kg-1) i medetomidina (0.1 mg kg-1) za indukciju, oba u niskim dozama, nakon čega je anestezija održavana primenom izoflurana u koncentraciji od 3% (podešen na isparavanje) u 100% kiseoniku (0.5 L min-1). Dejstvo medetomidina je neutralizovano atipamezolom (0.2 mg kg-1), aplikovanim intramuskularno. Elektivna celioskopija je izvedena u skladu sa standardnim protokolima. Heparinizovani uzorci krvi su uzeti jedan dan pre uvođenja u anesteziju venepunkcijom iz subkarapacijalnog venskog pleksusa. Drugo uzorkovanje krvi je uzvršeno tri sata nakon administracije anestetika. 
Rezultati i zaključak. GPx3 aktivnost u krvnoj plazmi (n=8) je izmerena primenom kuplovane reakcije, kao što su opisali Günzler i sar. (1974). Normalna distribucija podataka je testirana pomoću Shapiro - Wilk testa normalnosti (p>0,05), a grupe su poređene koristeći t test za zavisne uzorke. 
Aktivnost GPx3 u krvnoj plazmi je bila značajno viša (p=0.009) u uzorcima uzetim nakon trosatnog perioda oporavka od inhalacione anestezije aplikovane radi izvođenja elektivne celioskopije, nego u uzorcima uzetim pre aplikovanja anestezije. Vrednosti GPx3 izmerene nakon anestezije bile su u proseku 80% više od vrednosti pre anestezije. Može se zaključiti da je statistički značajan porast u aktivnosti GPx3 u plazmi od 91.02±36.05 mKat L-1, pre anestezije do 160.21±58.94 mKat L-1, tri sata nakon anestezije uzrokovan promenom u saturaciji kiseonika. Tokom intervencije saturacija kiseonika se povećava i do 100% čime se kornjače uvode u uslove visoke saturacije kiseonikom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Plasma glutathione peroxidase (GPx 3) activity in the freshwater turtle trachemys scripta elegans after isoflurane inhalation anesthesia
T1  - Aktivnost glutation peroksidaze (GPx 3) u plazmi semiakvatičnih kornjača trachemys scripta elegans nakon inhalacione anestezije izofluranom
VL  - 75
IS  - 1
SP  - 76
EP  - 82
DO  - 10.2298/VETGL181208005V
ER  - 

@article{
author = "Valčić, Olivera and Milanović, Svetlana and Došenović, Milan and Özvegy, József and Radaković, Milena and Vejnović, Branislav and Vučićević, Miloš",
year = "2021",
abstract = "Introduction. Glutathione peroxidases are selenoenzymes which have a crucial role in the protection of animals against oxidative stress. 
Materials and Methods. From September 2017 to April 2018, a group of eight red-eared sliders were admitted at the Clinic for Small Animals, Faculty of Veterinary Medicine, University of Belgrade for elective diagnostic celioscopy. The turtles were of unknown age, weight from 1.20 kg to 1.86 kg. The anesthesia protocol involved using ketamine and medetomidine, both at a low dosage (10 mg kg-1 and 0.1 mg kg-1, respectively) as induction, after which anesthesia was maintained using isoflurane at 3% (vapor setting) in 100% oxygen (0.5 L min-1). Medetomidine was reversed with atipamezole (0.2 mg kg-1), given intramuscularly. The elective celioscopy was performed according to standard protocols. One day prior to anesthesia, heparinized blood samples were taken using the subcarapacial venous plexus for venipuncture. The second sampling took place three hours after the anesthetics were administered. 
Results and Conclusions. GPx3 activity in the blood plasmas (n=8) was measured by the coupled test as described by Günzler et al. (1974). Data were tested for normality by the Shapiro-Wilk normality test and the groups were compared using a paired t-test. 
Blood plasma GPx3 activity was significantly higher (p=0.009) after a three-hour recovery period from inhalation anesthesia performed for elective diagnostic celioscopy, than before anesthesia. The measured post-anesthesia GPx3 activities were, on average, 
80% higher than the measurements prior to anesthesia. It can be concluded that the statistically significant increase in the activity of plasma GPx3 from 91.02±36.05 mKat L-1 prior to anesthesia to 160.21±58.94 mKat L-1 three hours after anesthesia is due to the change in oxygen saturation. This is increased to 100% during the procedure, thus exposing the turtles to conditions of high oxygen saturation., Uvod. Glutation peroksidaze su selenoenzimi koji igraju ključnu ulogu u zaštiti životinja od oksidativnog stresa. 
Materijal i metode. Od septembra 2017. do aprila 2018. godine, grupa od osam crvenouhih kornjača je primljena na Kliniku za male životinje, Fakulteta Veterinarske Medicine, Univerziteta u Beogradu zbog izvođenja elektivne dijagnostičke celioskopije. Kornjače su bile nepoznate starosti, mase između 1,20 kg i 1,86 kg. Anastetički protokol je uključivao primenu ketamina (10 mg kg-1) i medetomidina (0.1 mg kg-1) za indukciju, oba u niskim dozama, nakon čega je anestezija održavana primenom izoflurana u koncentraciji od 3% (podešen na isparavanje) u 100% kiseoniku (0.5 L min-1). Dejstvo medetomidina je neutralizovano atipamezolom (0.2 mg kg-1), aplikovanim intramuskularno. Elektivna celioskopija je izvedena u skladu sa standardnim protokolima. Heparinizovani uzorci krvi su uzeti jedan dan pre uvođenja u anesteziju venepunkcijom iz subkarapacijalnog venskog pleksusa. Drugo uzorkovanje krvi je uzvršeno tri sata nakon administracije anestetika. 
Rezultati i zaključak. GPx3 aktivnost u krvnoj plazmi (n=8) je izmerena primenom kuplovane reakcije, kao što su opisali Günzler i sar. (1974). Normalna distribucija podataka je testirana pomoću Shapiro - Wilk testa normalnosti (p>0,05), a grupe su poređene koristeći t test za zavisne uzorke. 
Aktivnost GPx3 u krvnoj plazmi je bila značajno viša (p=0.009) u uzorcima uzetim nakon trosatnog perioda oporavka od inhalacione anestezije aplikovane radi izvođenja elektivne celioskopije, nego u uzorcima uzetim pre aplikovanja anestezije. Vrednosti GPx3 izmerene nakon anestezije bile su u proseku 80% više od vrednosti pre anestezije. Može se zaključiti da je statistički značajan porast u aktivnosti GPx3 u plazmi od 91.02±36.05 mKat L-1, pre anestezije do 160.21±58.94 mKat L-1, tri sata nakon anestezije uzrokovan promenom u saturaciji kiseonika. Tokom intervencije saturacija kiseonika se povećava i do 100% čime se kornjače uvode u uslove visoke saturacije kiseonikom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Plasma glutathione peroxidase (GPx 3) activity in the freshwater turtle trachemys scripta elegans after isoflurane inhalation anesthesia, Aktivnost glutation peroksidaze (GPx 3) u plazmi semiakvatičnih kornjača trachemys scripta elegans nakon inhalacione anestezije izofluranom",
volume = "75",
number = "1",
pages = "76-82",
doi = "10.2298/VETGL181208005V"
}

Valčić, O., Milanović, S., Došenović, M., Özvegy, J., Radaković, M., Vejnović, B.,& Vučićević, M.. (2021). Plasma glutathione peroxidase (GPx 3) activity in the freshwater turtle trachemys scripta elegans after isoflurane inhalation anesthesia. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 75(1), 76-82.
https://doi.org/10.2298/VETGL181208005V

Valčić O, Milanović S, Došenović M, Özvegy J, Radaković M, Vejnović B, Vučićević M. Plasma glutathione peroxidase (GPx 3) activity in the freshwater turtle trachemys scripta elegans after isoflurane inhalation anesthesia. in Veterinarski Glasnik. 2021;75(1):76-82.
doi:10.2298/VETGL181208005V .

Valčić, Olivera, Milanović, Svetlana, Došenović, Milan, Özvegy, József, Radaković, Milena, Vejnović, Branislav, Vučićević, Miloš, "Plasma glutathione peroxidase (GPx 3) activity in the freshwater turtle trachemys scripta elegans after isoflurane inhalation anesthesia" in Veterinarski Glasnik, 75, no. 1 (2021):76-82,
https://doi.org/10.2298/VETGL181208005V . .

TY  - CONF
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3202
AB  - Mleko magarice se od davnina koristi u medicinske i kozmetičke svrhe, međutim
malo je bilo poznato koji molekuli su odgovorni za njegove protivupalne i
antimikrobne efekte. Poslednjih decenija, interesovanje naučnika za magareće
mleko je poraslo što je rezultiralo boljim poznavanjam njegovog sastava i
karakteristika. Po svom sastavu, magareće mleko je blisko mleku žene, prvenstveno
zbog niskog sadržaja masti i kazeina. Istovremeno, zahvaljujući karakteristikama
proteinskih frakcija, ono je pogodno za primenu kod osoba koje su
alergične na kravlje mleko. Ustanovljeno je prisustvo niza molekula, posebno
proteina i peptida, koji imaju anti-inflamatorno i antimikrobno dejstvo. Među
njima se posebno ističu lizozim, laktoferin, i laktoperoksidaza. U odnosu na humano
i kravlje mleko, mleko magarice je posebno bogato lizozimom. U ovom
radu su detaljnije opisane karakteristike i mehanizam delovanja navedenih
proteina.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021
T1  - Antiinflamatorni i antimikrobni efekti magarećeg mleka
SP  - 431
EP  - 439
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3202
ER  - 

@conference{
author = "Valčić, Olivera and Milanović, Svetlana",
year = "2021",
abstract = "Mleko magarice se od davnina koristi u medicinske i kozmetičke svrhe, međutim
malo je bilo poznato koji molekuli su odgovorni za njegove protivupalne i
antimikrobne efekte. Poslednjih decenija, interesovanje naučnika za magareće
mleko je poraslo što je rezultiralo boljim poznavanjam njegovog sastava i
karakteristika. Po svom sastavu, magareće mleko je blisko mleku žene, prvenstveno
zbog niskog sadržaja masti i kazeina. Istovremeno, zahvaljujući karakteristikama
proteinskih frakcija, ono je pogodno za primenu kod osoba koje su
alergične na kravlje mleko. Ustanovljeno je prisustvo niza molekula, posebno
proteina i peptida, koji imaju anti-inflamatorno i antimikrobno dejstvo. Među
njima se posebno ističu lizozim, laktoferin, i laktoperoksidaza. U odnosu na humano
i kravlje mleko, mleko magarice je posebno bogato lizozimom. U ovom
radu su detaljnije opisane karakteristike i mehanizam delovanja navedenih
proteina.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021",
title = "Antiinflamatorni i antimikrobni efekti magarećeg mleka",
pages = "431-439",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3202"
}

Valčić, O.,& Milanović, S.. (2021). Antiinflamatorni i antimikrobni efekti magarećeg mleka. in Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021
Beograd : Srpsko veterinarsko društvo., 431-439.
https://hdl.handle.net/21.15107/rcub_veterinar_3202

Valčić O, Milanović S. Antiinflamatorni i antimikrobni efekti magarećeg mleka. in Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021. 2021;:431-439.
https://hdl.handle.net/21.15107/rcub_veterinar_3202 .

Valčić, Olivera, Milanović, Svetlana, "Antiinflamatorni i antimikrobni efekti magarećeg mleka" in Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021 (2021):431-439,
https://hdl.handle.net/21.15107/rcub_veterinar_3202 .

TY  - CONF
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3410
AB  - Заједно са селеном, цинком, бакром, јодом и др. молибден спада у есенцијалне
микроелементе који су неопходни за нормално одвијање метаболичких
процеса. У природи се молибден не налази у елементарном стању већ је
првенствено присутан у облику молибдената (MoS2) и феримолибдата
(Fe2(MoO4)2), док је у мешаоницама сточне хране чест суплемент у облику
натријум молибдата. У природи добар извор молибдена за домаће животиње
представљају биљна хранива (цереалије) и лиснато поврће у којима је
просечан садржај молибдена од 0,5 до 100 мг/кг СМ. Забележена су тровања
животиња која су на испаши где су присутне биљке са високим садржајем овог
микроелемента. Добро се ресорбује у танким цревима, док је у ткивима
присутан првенствено у јетри, бубрезима и панкреасу. Полуживот у
организму је кратак, износи свега неколико часова и излучује се преко урина.
Код животиња, молибден улази у састав четири различита ензима
(металофлавопротеина): ксантин оксидазе, алдехид оксидазе, сулфит
оксидазе и митохондријална амидоксин редуктаза (мАМЦ). У наведеним
ензимима молибден је присутан у облику комплекса молибдоптерина преко
којег учествује у реакцијама хидроксилације.
Клиничка слика дефицита молибдена забележена је код коза, јагњади, и
живине. Појава патологије везане за дефицит није увек последица недовољног
уноса, већ може бити и последица генетски контролисане неадекватне синтезе
и уградње молибдоптерина. Значајно већи и чешћи проблем представља
питање токсичности молибдена. Краве и овце су осетљиве на дозе до 30 мг/кг,
док коњи, свиње и козе толеришу дозе до 1000 мг/кг. Молибденоза код говеда
настаје након уноса високих доза молибдена, уз настанак тритиомолибдата, и
уз истовремено ниске дозе бакра. Симптоми укључују анемију,
гастроинтестиналне сметње, смањен раст и отежану репродукцију. Код телади
забележена је перидонтална инфламација и губитак зуба.
Забележене су сложене интеракције између молибдена, бакра и сумпора.
Ефекти молибдена се могу објаснити променама афинитета лиганда за бакар,
услед чега долази до поремећаја дистрибуције бакра и његовог метаболичког
дефицита. Молибден је антидот у случају тровања бакром, међутим
механизам деловања још увек није у потпуности расветљен.
AB  - Together with selenium, zinc, copper, iodine, etc. molybdenum is an essential
microelement required for metabolic processes in the cell. In nature molybdenum
is not present in its pure elementary form, but as molybdenate (MoS2) and
ferrimoyibdate (Fe2(MoO4)2), while in the feedstuff industry is a common
supplement in the form of sodium molybdate. A good source of molybdenum for
animals are plant feedstuffs (cereals) and leafy vegetables. In these plants the
average molybdenum concentration ranges from 0.5 to 100 mg/kg DM. Cases of
poisoning have been reported in situations where the animals were on pasture
grazing on plants with a high content of this microelement. It is well absorbed in
the small intestine, and is mainly present in the organism in the liver, kidneys and
pancreas. Its half-life in the organism is short, only a few hours, and is excreted by
means of urine.
In the animal kingdom molybdenum is a component of four different enzymes
(metaloflavoproteins): xanthine oxidase, aldehyde oxidase, sulphite oxidase, and
mitochondrial amidoxin reductase (mAMC). In these enzymes molybdenum is
present as a molybdopterine complex by means of which takes part in
hydroxylation reactions.
Clinically manifested molybdenum deficiency has been recorded in goats, lambs,
and poultry. The existence of pathological signs is not always the result of an
inadequate intake, as it can be the consequence of genetically controlled
inadequate synthesis and incorporation of molybdopteridine. A significantly
more evident problem is presented as the result of molybdenum toxicity. Cattle
and sheep are sensitive to doses of about 30 mg/kg, but horses, pigs and goats can
tolerate up to 1000 mg/kg. Molybdenosis in cattle due to high doses of
molybdenum develops as tritiomolibdate forms concurrent to low copper doses.
The symptoms include anemia, gastrointestinal disorders, stunt growth and poor
reproduction. Calves may develop periodontal inflammation and teeth loss.
Complex interactions between molybdenum, copper and sulphur are
documented. The effects of molybdenum can be explained by changes of the
affinity of copper ligands which results in changes of copper distribution and
metabolic deficiency. Molibdenum is an effective antidote for copper poisoning,
however the mechanism of action is still not elucidated.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 26. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (BiH), Teslić, 9 - 12. jun 2021
T1  - Zaboravljeni esencijalni mikroelement- molibden
T1  - The forgotten essential microelement- molybdenum
SP  - 175
EP  - 176
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3410
ER  - 

@conference{
author = "Valčić, Olivera and Milanović, Svetlana",
year = "2021",
abstract = "Заједно са селеном, цинком, бакром, јодом и др. молибден спада у есенцијалне
микроелементе који су неопходни за нормално одвијање метаболичких
процеса. У природи се молибден не налази у елементарном стању већ је
првенствено присутан у облику молибдената (MoS2) и феримолибдата
(Fe2(MoO4)2), док је у мешаоницама сточне хране чест суплемент у облику
натријум молибдата. У природи добар извор молибдена за домаће животиње
представљају биљна хранива (цереалије) и лиснато поврће у којима је
просечан садржај молибдена од 0,5 до 100 мг/кг СМ. Забележена су тровања
животиња која су на испаши где су присутне биљке са високим садржајем овог
микроелемента. Добро се ресорбује у танким цревима, док је у ткивима
присутан првенствено у јетри, бубрезима и панкреасу. Полуживот у
организму је кратак, износи свега неколико часова и излучује се преко урина.
Код животиња, молибден улази у састав четири различита ензима
(металофлавопротеина): ксантин оксидазе, алдехид оксидазе, сулфит
оксидазе и митохондријална амидоксин редуктаза (мАМЦ). У наведеним
ензимима молибден је присутан у облику комплекса молибдоптерина преко
којег учествује у реакцијама хидроксилације.
Клиничка слика дефицита молибдена забележена је код коза, јагњади, и
живине. Појава патологије везане за дефицит није увек последица недовољног
уноса, већ може бити и последица генетски контролисане неадекватне синтезе
и уградње молибдоптерина. Значајно већи и чешћи проблем представља
питање токсичности молибдена. Краве и овце су осетљиве на дозе до 30 мг/кг,
док коњи, свиње и козе толеришу дозе до 1000 мг/кг. Молибденоза код говеда
настаје након уноса високих доза молибдена, уз настанак тритиомолибдата, и
уз истовремено ниске дозе бакра. Симптоми укључују анемију,
гастроинтестиналне сметње, смањен раст и отежану репродукцију. Код телади
забележена је перидонтална инфламација и губитак зуба.
Забележене су сложене интеракције између молибдена, бакра и сумпора.
Ефекти молибдена се могу објаснити променама афинитета лиганда за бакар,
услед чега долази до поремећаја дистрибуције бакра и његовог метаболичког
дефицита. Молибден је антидот у случају тровања бакром, међутим
механизам деловања још увек није у потпуности расветљен., Together with selenium, zinc, copper, iodine, etc. molybdenum is an essential
microelement required for metabolic processes in the cell. In nature molybdenum
is not present in its pure elementary form, but as molybdenate (MoS2) and
ferrimoyibdate (Fe2(MoO4)2), while in the feedstuff industry is a common
supplement in the form of sodium molybdate. A good source of molybdenum for
animals are plant feedstuffs (cereals) and leafy vegetables. In these plants the
average molybdenum concentration ranges from 0.5 to 100 mg/kg DM. Cases of
poisoning have been reported in situations where the animals were on pasture
grazing on plants with a high content of this microelement. It is well absorbed in
the small intestine, and is mainly present in the organism in the liver, kidneys and
pancreas. Its half-life in the organism is short, only a few hours, and is excreted by
means of urine.
In the animal kingdom molybdenum is a component of four different enzymes
(metaloflavoproteins): xanthine oxidase, aldehyde oxidase, sulphite oxidase, and
mitochondrial amidoxin reductase (mAMC). In these enzymes molybdenum is
present as a molybdopterine complex by means of which takes part in
hydroxylation reactions.
Clinically manifested molybdenum deficiency has been recorded in goats, lambs,
and poultry. The existence of pathological signs is not always the result of an
inadequate intake, as it can be the consequence of genetically controlled
inadequate synthesis and incorporation of molybdopteridine. A significantly
more evident problem is presented as the result of molybdenum toxicity. Cattle
and sheep are sensitive to doses of about 30 mg/kg, but horses, pigs and goats can
tolerate up to 1000 mg/kg. Molybdenosis in cattle due to high doses of
molybdenum develops as tritiomolibdate forms concurrent to low copper doses.
The symptoms include anemia, gastrointestinal disorders, stunt growth and poor
reproduction. Calves may develop periodontal inflammation and teeth loss.
Complex interactions between molybdenum, copper and sulphur are
documented. The effects of molybdenum can be explained by changes of the
affinity of copper ligands which results in changes of copper distribution and
metabolic deficiency. Molibdenum is an effective antidote for copper poisoning,
however the mechanism of action is still not elucidated.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "26. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (BiH), Teslić, 9 - 12. jun 2021",
title = "Zaboravljeni esencijalni mikroelement- molibden, The forgotten essential microelement- molybdenum",
pages = "175-176",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3410"
}

Valčić, O.,& Milanović, S.. (2021). Zaboravljeni esencijalni mikroelement- molibden. in 26. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (BiH), Teslić, 9 - 12. jun 2021
Banja Luka : Veterinarska komora Republike Srpske., 175-176.
https://hdl.handle.net/21.15107/rcub_veterinar_3410

Valčić O, Milanović S. Zaboravljeni esencijalni mikroelement- molibden. in 26. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (BiH), Teslić, 9 - 12. jun 2021. 2021;:175-176.
https://hdl.handle.net/21.15107/rcub_veterinar_3410 .

Valčić, Olivera, Milanović, Svetlana, "Zaboravljeni esencijalni mikroelement- molibden" in 26. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (BiH), Teslić, 9 - 12. jun 2021 (2021):175-176,
https://hdl.handle.net/21.15107/rcub_veterinar_3410 .

TY  - CONF
AU  - Milanović, Svetlana
AU  - Valčić, Olivera
AU  - Jovanović, Ivan
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3577
AB  - Intenzivna živinarska proizvodnja nosi sa sobom puno izazova, jer je potrebno
za što kraće vreme proizvesti kvalitetan i zdravstveno ispravan proizvod. Kako
bi se maksimalno iskoristio genetski potencijal jedinki, nameće se upotreba
kvalitetnih hraniva koja će u potpunosti zadovoljiti njihove osnovne i proizvodne potrebe. Poseban izazov predstavlja način držanja živine u intenzivnoj proizvodnji koji pogoduje brzom širenju zaraznih bolesti. Zbog svega navedenog,
hrana za živinu sadrži mnoge dodatke koji će poboljšati proizvodne karakteristike, a takođe, uticati na uspešniji razvoj imuniteta nakon vakcinacije i odbranu organizma u slučaju pojave infekcije.
U intenzivnoj proizvodnji dodavanje mikroelemenata predstavlja jedan od
ključnih faktora potrebnih za funkcionisanje organizma. U ovom radu će fokus
biti na njihovom uticaju na imunitet.
PB  - Beograd : Ciiip Živinarstvo
C3  - XXIX Savetovanje živinara, online, 5. 11. 2020
T1  - Uloga i značaj mikroelemenata za imunitet živine
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3577
ER  - 

@conference{
author = "Milanović, Svetlana and Valčić, Olivera and Jovanović, Ivan",
year = "2021",
abstract = "Intenzivna živinarska proizvodnja nosi sa sobom puno izazova, jer je potrebno
za što kraće vreme proizvesti kvalitetan i zdravstveno ispravan proizvod. Kako
bi se maksimalno iskoristio genetski potencijal jedinki, nameće se upotreba
kvalitetnih hraniva koja će u potpunosti zadovoljiti njihove osnovne i proizvodne potrebe. Poseban izazov predstavlja način držanja živine u intenzivnoj proizvodnji koji pogoduje brzom širenju zaraznih bolesti. Zbog svega navedenog,
hrana za živinu sadrži mnoge dodatke koji će poboljšati proizvodne karakteristike, a takođe, uticati na uspešniji razvoj imuniteta nakon vakcinacije i odbranu organizma u slučaju pojave infekcije.
U intenzivnoj proizvodnji dodavanje mikroelemenata predstavlja jedan od
ključnih faktora potrebnih za funkcionisanje organizma. U ovom radu će fokus
biti na njihovom uticaju na imunitet.",
publisher = "Beograd : Ciiip Živinarstvo",
journal = "XXIX Savetovanje živinara, online, 5. 11. 2020",
title = "Uloga i značaj mikroelemenata za imunitet živine",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3577"
}

Milanović, S., Valčić, O.,& Jovanović, I.. (2021). Uloga i značaj mikroelemenata za imunitet živine. in XXIX Savetovanje živinara, online, 5. 11. 2020
Beograd : Ciiip Živinarstvo..
https://hdl.handle.net/21.15107/rcub_veterinar_3577

Milanović S, Valčić O, Jovanović I. Uloga i značaj mikroelemenata za imunitet živine. in XXIX Savetovanje živinara, online, 5. 11. 2020. 2021;.
https://hdl.handle.net/21.15107/rcub_veterinar_3577 .

Milanović, Svetlana, Valčić, Olivera, Jovanović, Ivan, "Uloga i značaj mikroelemenata za imunitet živine" in XXIX Savetovanje živinara, online, 5. 11. 2020 (2021),
https://hdl.handle.net/21.15107/rcub_veterinar_3577 .

TY  - CONF
AU  - Milanović, Svetlana
AU  - Valčić, Olivera
AU  - Jovanović, Ivan
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3578
AB  - Selen je mikroelement koji ulazi u sastav mnogih proteina i enzima neophodnih
u odbrani od oksidativnog stresa, aktivaciji i deaktivaciji tireoidnih
hormona i adekvatnom imunskom odgovoru. Poseban izazov u intenzivnoj
živinarskoj proizvodnji predstavlja način držanja ptica, koji pogoduje širenju
zaraznih bolesti, te je pravilna i balansirana ishrana, osim za zadovoljenje
osnovnih i proizvodnih potreba jedinki, neophodna i za pravilan rad imunskog
sistema. Selen je, pored ostalih mikroelemenata, izuzetno važan u tom smislu.
On kao gradivni element proteina i enzima utiče na gotovo sve segmente imunskog
sistema (urođeni i stečeni, celularni i humoralni).
Ova činjenica je važna jer dodavanje u hranu selena u obliku selenocisteina i
selenometionina može imati različit efekat zbog toga što se metabolizam ovih
aminokiselina i neorganskog selena vrlo razlikuju.
PB  - Beograd : Ciiip Živinarstvo
C3  - XXX Savetovanja živinara, Tara, 21 - 24. septembar 2021
T1  - Uticaj selena na imunski sistem živine
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3578
ER  - 

@conference{
author = "Milanović, Svetlana and Valčić, Olivera and Jovanović, Ivan",
year = "2021",
abstract = "Selen je mikroelement koji ulazi u sastav mnogih proteina i enzima neophodnih
u odbrani od oksidativnog stresa, aktivaciji i deaktivaciji tireoidnih
hormona i adekvatnom imunskom odgovoru. Poseban izazov u intenzivnoj
živinarskoj proizvodnji predstavlja način držanja ptica, koji pogoduje širenju
zaraznih bolesti, te je pravilna i balansirana ishrana, osim za zadovoljenje
osnovnih i proizvodnih potreba jedinki, neophodna i za pravilan rad imunskog
sistema. Selen je, pored ostalih mikroelemenata, izuzetno važan u tom smislu.
On kao gradivni element proteina i enzima utiče na gotovo sve segmente imunskog
sistema (urođeni i stečeni, celularni i humoralni).
Ova činjenica je važna jer dodavanje u hranu selena u obliku selenocisteina i
selenometionina može imati različit efekat zbog toga što se metabolizam ovih
aminokiselina i neorganskog selena vrlo razlikuju.",
publisher = "Beograd : Ciiip Živinarstvo",
journal = "XXX Savetovanja živinara, Tara, 21 - 24. septembar 2021",
title = "Uticaj selena na imunski sistem živine",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3578"
}

Milanović, S., Valčić, O.,& Jovanović, I.. (2021). Uticaj selena na imunski sistem živine. in XXX Savetovanja živinara, Tara, 21 - 24. septembar 2021
Beograd : Ciiip Živinarstvo..
https://hdl.handle.net/21.15107/rcub_veterinar_3578

Milanović S, Valčić O, Jovanović I. Uticaj selena na imunski sistem živine. in XXX Savetovanja živinara, Tara, 21 - 24. septembar 2021. 2021;.
https://hdl.handle.net/21.15107/rcub_veterinar_3578 .

Milanović, Svetlana, Valčić, Olivera, Jovanović, Ivan, "Uticaj selena na imunski sistem živine" in XXX Savetovanja živinara, Tara, 21 - 24. septembar 2021 (2021),
https://hdl.handle.net/21.15107/rcub_veterinar_3578 .

TY  - CONF
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3371
AB  - Zahtevi za sve većim proizvodnim rezultatima, intenzivna selekcija, kao i globalno
otopljavanje doveli su do pojave toplotnog stresa. Predhodna saznanja su tumačila
nastali pad proizvodnih rezultata isključivo kao posledica inapetence usled
visokih temperatura. Međutim, toplotni stres dovodi do niza poremećaja na nivou
fiziološke homeostaze i metaboličkih procesa. Selekcija u cilju veće proizvodnje
mišićne mase je rezultirala povećanom proizvodnjom metaboličke toplote, usled
čega dolazi do hiperventilacije i posledične metaboličke acidoze što dodatno
opterećuje organizam. Zbog nastalog toplotnog stresa, uprkos smanjenom unosu
hrane, dolazi do smanjenog lipolitičkog kapaciteta masnog tkiva i smanjene
koncentracije ne-esterifikovanih masnih kiselina (NEFA) u krvnoj plazmi goveda i
svinja. Kao odgovor na ćelijski stres dolazi do porasta koncentracije insulina i
povećanih potreba za glukozom radi očuvanja normoglikemije. Istovremeno,
značajno opada aktivnost tireoidnih hormona T3 i T4. Preraspodela cirkulacije
koja nastaje usled toplotnog stresa dovodi do mobilizacije krvi iz digestivnog
trakta što dovodi do hipoksije creva usled koje se javlja poremećaj propustljivosti
sluzokože i prolaska lipopolisaharida bakterija u portalni i sistemski krvotok.
Paralelno sa navedenim procesima dolazi do intenzivne glikogenolize u mišićima
usled aktivacije glikogen fosforilaze. Toplotni stres je jedan od pokretača procesa
nastanka slobodnih kiseoničnih radikala, oksidativnog stresa i sinteze
malonildialdehida koji dodatno oštećuju integritet ćelijskih membrana.
Biohemijski profil krvi goveda, svinja i živine ukazuje na porast koncentracije
proteina toplotnog šoka (HSP), proizvoda peroksidacije masnih kiselina (MDA),
enzima zaštite od oksidativnog stresa (SOD, GPx), kao i hemoglobina.
Navedeni poremećaji dovode do promene kvaliteta goveđeg (DFD- dry firm dark
meat) i svinjskog mesa (PSE- pale soft exudative) što rezultira značajnim
ekonomskim gubitcima u proizvodnji i plasmanu.
Usled navedenog, neophodno je dublje sagledati problematiku toplotnog stresa u
uslovima intenzivne proizvodnje kako bi se negativni efekti maksimalno sprečili
ili ublažili.
AB  - Demands for increasing production results, as well as global warming have led to
the emergence of heat stress. Previous knowledge interpreted the resulting
decline in production solely as a consequence of a decreased appetite. However,
heat stress leads to a number of disorders at the level of physiological homeostasis
and metabolic processes. Selection for higher muscle mass resulted in increased
metabolic heat production. Due to the increase in heat, hyperventilation and
consequent metabolic acidosis occur, which additionally burdens the organism.
Due to the resulting heat stress, despite the reduced food intake, there is a reduced
lipolytic capacity of adipose tissue and a reduced concentration of non-esterified
fatty acids (NEFA) in the blood plasma of cattle and pigs. In response to cellular
stress, insulin concentrations increase and glucose needs increase in order to
preserve normoglycemia. At the same time, the activity of thyroid hormones T3
and T4 decreases significantly. The redistribution of circulation caused by heat
stress leads to the mobilization of blood from the digestive tract, which results in
intestinal hypoxia, which results in increased permeability of the mucous
membrane and the passage of bacterial lipopolysaccharides into the portal and
systemic bloodstream. In parallel with the mentioned processes, intensive
glycogenolysis occurs in the muscles due to the activation of glycogen
phosphorylase. Heat stress is one of the initiators of the process of formation of
free oxygen radicals, oxidative stress and synthesis of malonyldialdehyde, which
additionally damage the integrity of cell membranes. The biochemical profile of
the blood of cattle, pigs and poultry indicates an increase in the concentration of
heat shock protein (HSP), fatty acid peroxidation products (MDA), enzymes
involved in the protection against oxidative stress (SOD, GPx), as well as
hemoglobin.
These disorders lead to changes in the quality of beef (DFD - dry firm dark meat)
and pork (PSE - pale soft exudative), which results in significant economic losses
in production and marketing.
Due to the above, it is necessary to look deeper into the issue of heat stress in
conditions of intensive production in order to maximally prevent or mitigate the
negative effects.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 25. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), naučni skup sa međunarodnim učešćem, Teslić,  2020
T1  - Efekti toplotnog stresa na metabolizam farmskih životinja
T1  - Effects of heat stress on metabolism in farm animals
SP  - 152
EP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3371
ER  - 

@conference{
author = "Valčić, Olivera and Milanović, Svetlana",
year = "2020",
abstract = "Zahtevi za sve većim proizvodnim rezultatima, intenzivna selekcija, kao i globalno
otopljavanje doveli su do pojave toplotnog stresa. Predhodna saznanja su tumačila
nastali pad proizvodnih rezultata isključivo kao posledica inapetence usled
visokih temperatura. Međutim, toplotni stres dovodi do niza poremećaja na nivou
fiziološke homeostaze i metaboličkih procesa. Selekcija u cilju veće proizvodnje
mišićne mase je rezultirala povećanom proizvodnjom metaboličke toplote, usled
čega dolazi do hiperventilacije i posledične metaboličke acidoze što dodatno
opterećuje organizam. Zbog nastalog toplotnog stresa, uprkos smanjenom unosu
hrane, dolazi do smanjenog lipolitičkog kapaciteta masnog tkiva i smanjene
koncentracije ne-esterifikovanih masnih kiselina (NEFA) u krvnoj plazmi goveda i
svinja. Kao odgovor na ćelijski stres dolazi do porasta koncentracije insulina i
povećanih potreba za glukozom radi očuvanja normoglikemije. Istovremeno,
značajno opada aktivnost tireoidnih hormona T3 i T4. Preraspodela cirkulacije
koja nastaje usled toplotnog stresa dovodi do mobilizacije krvi iz digestivnog
trakta što dovodi do hipoksije creva usled koje se javlja poremećaj propustljivosti
sluzokože i prolaska lipopolisaharida bakterija u portalni i sistemski krvotok.
Paralelno sa navedenim procesima dolazi do intenzivne glikogenolize u mišićima
usled aktivacije glikogen fosforilaze. Toplotni stres je jedan od pokretača procesa
nastanka slobodnih kiseoničnih radikala, oksidativnog stresa i sinteze
malonildialdehida koji dodatno oštećuju integritet ćelijskih membrana.
Biohemijski profil krvi goveda, svinja i živine ukazuje na porast koncentracije
proteina toplotnog šoka (HSP), proizvoda peroksidacije masnih kiselina (MDA),
enzima zaštite od oksidativnog stresa (SOD, GPx), kao i hemoglobina.
Navedeni poremećaji dovode do promene kvaliteta goveđeg (DFD- dry firm dark
meat) i svinjskog mesa (PSE- pale soft exudative) što rezultira značajnim
ekonomskim gubitcima u proizvodnji i plasmanu.
Usled navedenog, neophodno je dublje sagledati problematiku toplotnog stresa u
uslovima intenzivne proizvodnje kako bi se negativni efekti maksimalno sprečili
ili ublažili., Demands for increasing production results, as well as global warming have led to
the emergence of heat stress. Previous knowledge interpreted the resulting
decline in production solely as a consequence of a decreased appetite. However,
heat stress leads to a number of disorders at the level of physiological homeostasis
and metabolic processes. Selection for higher muscle mass resulted in increased
metabolic heat production. Due to the increase in heat, hyperventilation and
consequent metabolic acidosis occur, which additionally burdens the organism.
Due to the resulting heat stress, despite the reduced food intake, there is a reduced
lipolytic capacity of adipose tissue and a reduced concentration of non-esterified
fatty acids (NEFA) in the blood plasma of cattle and pigs. In response to cellular
stress, insulin concentrations increase and glucose needs increase in order to
preserve normoglycemia. At the same time, the activity of thyroid hormones T3
and T4 decreases significantly. The redistribution of circulation caused by heat
stress leads to the mobilization of blood from the digestive tract, which results in
intestinal hypoxia, which results in increased permeability of the mucous
membrane and the passage of bacterial lipopolysaccharides into the portal and
systemic bloodstream. In parallel with the mentioned processes, intensive
glycogenolysis occurs in the muscles due to the activation of glycogen
phosphorylase. Heat stress is one of the initiators of the process of formation of
free oxygen radicals, oxidative stress and synthesis of malonyldialdehyde, which
additionally damage the integrity of cell membranes. The biochemical profile of
the blood of cattle, pigs and poultry indicates an increase in the concentration of
heat shock protein (HSP), fatty acid peroxidation products (MDA), enzymes
involved in the protection against oxidative stress (SOD, GPx), as well as
hemoglobin.
These disorders lead to changes in the quality of beef (DFD - dry firm dark meat)
and pork (PSE - pale soft exudative), which results in significant economic losses
in production and marketing.
Due to the above, it is necessary to look deeper into the issue of heat stress in
conditions of intensive production in order to maximally prevent or mitigate the
negative effects.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "25. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), naučni skup sa međunarodnim učešćem, Teslić,  2020",
title = "Efekti toplotnog stresa na metabolizam farmskih životinja, Effects of heat stress on metabolism in farm animals",
pages = "152-153",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3371"
}

Valčić, O.,& Milanović, S.. (2020). Efekti toplotnog stresa na metabolizam farmskih životinja. in 25. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), naučni skup sa međunarodnim učešćem, Teslić,  2020
Banja Luka : Veterinarska komora Republike Srpske., 152-153.
https://hdl.handle.net/21.15107/rcub_veterinar_3371

Valčić O, Milanović S. Efekti toplotnog stresa na metabolizam farmskih životinja. in 25. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), naučni skup sa međunarodnim učešćem, Teslić,  2020. 2020;:152-153.
https://hdl.handle.net/21.15107/rcub_veterinar_3371 .

Valčić, Olivera, Milanović, Svetlana, "Efekti toplotnog stresa na metabolizam farmskih životinja" in 25. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), naučni skup sa međunarodnim učešćem, Teslić,  2020 (2020):152-153,
https://hdl.handle.net/21.15107/rcub_veterinar_3371 .

TY  - JOUR
AU  - Žutić, Jadranka
AU  - Valčić, Olivera
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Kureljušić, Jasna
AU  - Bacić, Dragan
AU  - Jezdimirović, Nemanja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1796
AB  - A total of 120 Escherichia coli (E. coli) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrial and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (33.33%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrial and toxin genes were found. The most common virulence pattern was F4/STb/LT/EAST1 detected in 23.45% of E. coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from diarrhoeic suckling piglets in Serbia.
PB  - Polish Soc Veterinary Sciences Editorial Office, Lublin
T2  - Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice
T1  - Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia
VL  - 76
IS  - 1
SP  - 41
EP  - 45
DO  - 10.21521/mw.6326
ER  - 

@article{
author = "Žutić, Jadranka and Valčić, Olivera and Milićević, Vesna and Veljović, Ljubiša and Kureljušić, Jasna and Bacić, Dragan and Jezdimirović, Nemanja",
year = "2020",
abstract = "A total of 120 Escherichia coli (E. coli) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrial and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (33.33%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrial and toxin genes were found. The most common virulence pattern was F4/STb/LT/EAST1 detected in 23.45% of E. coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from diarrhoeic suckling piglets in Serbia.",
publisher = "Polish Soc Veterinary Sciences Editorial Office, Lublin",
journal = "Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice",
title = "Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia",
volume = "76",
number = "1",
pages = "41-45",
doi = "10.21521/mw.6326"
}

Žutić, J., Valčić, O., Milićević, V., Veljović, L., Kureljušić, J., Bacić, D.,& Jezdimirović, N.. (2020). Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice
Polish Soc Veterinary Sciences Editorial Office, Lublin., 76(1), 41-45.
https://doi.org/10.21521/mw.6326

Žutić J, Valčić O, Milićević V, Veljović L, Kureljušić J, Bacić D, Jezdimirović N. Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice. 2020;76(1):41-45.
doi:10.21521/mw.6326 .

Žutić, Jadranka, Valčić, Olivera, Milićević, Vesna, Veljović, Ljubiša, Kureljušić, Jasna, Bacić, Dragan, Jezdimirović, Nemanja, "Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia" in Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice, 76, no. 1 (2020):41-45,
https://doi.org/10.21521/mw.6326 . .

TY  - JOUR
AU  - Živojinović, J.I.
AU  - Backović, D.
AU  - Belojević, G.
AU  - Valčić, Olivera
AU  - Soldatović, Ivan
AU  - Janković, J.
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1812
AB  - Background To the best of our knowledge, studies are lacking on burnout among veterinary students in Serbia, and this is the first study trying to address such a problem. Therefore, the aim of this cross-sectional study was to investigate the predictors of burnout among Belgrade veterinary students. Methods Maslach Burnout Inventory (MBI) and anonymous structured questionnaire addressed to personal data, health habits and stressful influence of educational process were applied among 496 respondents from a total of 1113 students from all grades in spring semester 2014 (response rate 44.6%). Results The prevalence of burnout was 43.3%. High scores on depersonalization and emotional exhaustion scales of MBI were found among 79.4% and 45.0% students, respectively; low personal accomplishment was reported by 50.5% students. Female students reported higher levels of emotional exhaustion compared to males (p = 0.012). A low score on personal achievement scale of MBI was least frequent among the freshmen and most frequent among sophomores (41.1% and 65.3%, respectively; p = 0.986). There were more students with burnout who considered their health as a worsened vs. pre-study period compared to students with no burnout, both concerning mental (61.4% vs. 38.6%) and physical health (61.1% vs. 38.9%; both p lt 0.001). There were more smokers among students who suffered from burnout compared to students who did not (52.0% vs. 48.0%; p = 0.013). A multiple regression revealed an independent dose-response effect of perceived stress at exams on the onset of burnout (moderate stress OR = 2.164 and high stress OR = 3.878). Also, students with the moderate and high stressful effect of communication with teaching staff, as well as, those with worse self-perceived physical and mental health had more than two times higher presence of burnout. Conclusions The prevalence of burnout among Belgrade veterinary students is relatively high. Primary prevention should be focused on the revealed predictors of burnout.
PB  - Public Library of Science
T2  - PLoS One
T1  - Predictors of burnout among Belgrade veterinary students: A cross-sectional study
VL  - 15
IS  - 3
SP  - e0230685
DO  - 10.1371/journal.pone.0230685
ER  - 

@article{
author = "Živojinović, J.I. and Backović, D. and Belojević, G. and Valčić, Olivera and Soldatović, Ivan and Janković, J.",
year = "2020",
abstract = "Background To the best of our knowledge, studies are lacking on burnout among veterinary students in Serbia, and this is the first study trying to address such a problem. Therefore, the aim of this cross-sectional study was to investigate the predictors of burnout among Belgrade veterinary students. Methods Maslach Burnout Inventory (MBI) and anonymous structured questionnaire addressed to personal data, health habits and stressful influence of educational process were applied among 496 respondents from a total of 1113 students from all grades in spring semester 2014 (response rate 44.6%). Results The prevalence of burnout was 43.3%. High scores on depersonalization and emotional exhaustion scales of MBI were found among 79.4% and 45.0% students, respectively; low personal accomplishment was reported by 50.5% students. Female students reported higher levels of emotional exhaustion compared to males (p = 0.012). A low score on personal achievement scale of MBI was least frequent among the freshmen and most frequent among sophomores (41.1% and 65.3%, respectively; p = 0.986). There were more students with burnout who considered their health as a worsened vs. pre-study period compared to students with no burnout, both concerning mental (61.4% vs. 38.6%) and physical health (61.1% vs. 38.9%; both p lt 0.001). There were more smokers among students who suffered from burnout compared to students who did not (52.0% vs. 48.0%; p = 0.013). A multiple regression revealed an independent dose-response effect of perceived stress at exams on the onset of burnout (moderate stress OR = 2.164 and high stress OR = 3.878). Also, students with the moderate and high stressful effect of communication with teaching staff, as well as, those with worse self-perceived physical and mental health had more than two times higher presence of burnout. Conclusions The prevalence of burnout among Belgrade veterinary students is relatively high. Primary prevention should be focused on the revealed predictors of burnout.",
publisher = "Public Library of Science",
journal = "PLoS One",
title = "Predictors of burnout among Belgrade veterinary students: A cross-sectional study",
volume = "15",
number = "3",
pages = "e0230685",
doi = "10.1371/journal.pone.0230685"
}

Živojinović, J.I., Backović, D., Belojević, G., Valčić, O., Soldatović, I.,& Janković, J.. (2020). Predictors of burnout among Belgrade veterinary students: A cross-sectional study. in PLoS One
Public Library of Science., 15(3), e0230685.
https://doi.org/10.1371/journal.pone.0230685

Živojinović J, Backović D, Belojević G, Valčić O, Soldatović I, Janković J. Predictors of burnout among Belgrade veterinary students: A cross-sectional study. in PLoS One. 2020;15(3):e0230685.
doi:10.1371/journal.pone.0230685 .

Živojinović, J.I., Backović, D., Belojević, G., Valčić, Olivera, Soldatović, Ivan, Janković, J., "Predictors of burnout among Belgrade veterinary students: A cross-sectional study" in PLoS One, 15, no. 3 (2020):e0230685,
https://doi.org/10.1371/journal.pone.0230685 . .

TY  - JOUR
AU  - Žutić, Jadranka
AU  - Valčić, Olivera
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Kureljušić, Jasna
AU  - Bacić, Dragan
AU  - Jezdimirović, Nemanja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2017
AB  - A total of 120 Escherichia coll (E. coif) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrlal and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (3333%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli Isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrlal and toxin genes were found. The most common virulence pattern was F4/STb/LT/EASTl detected in 23.45% of E coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from dlarrhoelc suckling piglets in Serbia. © 2020 Polskie Towarzystwo Nauk Weterynaryjnych.
PB  - Polskie Towarzystwo Nauk Weterynaryjnych (Polish Society of Veterinary Sciences)
T2  - Medycyna Weterynaryjna
T1  - Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia
VL  - 76
IS  - 1
SP  - 41
EP  - 45
DO  - 10.21521/mw.6326
ER  - 

@article{
author = "Žutić, Jadranka and Valčić, Olivera and Milićević, Vesna and Veljović, Ljubiša and Kureljušić, Jasna and Bacić, Dragan and Jezdimirović, Nemanja",
year = "2020",
abstract = "A total of 120 Escherichia coll (E. coif) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrlal and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (3333%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli Isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrlal and toxin genes were found. The most common virulence pattern was F4/STb/LT/EASTl detected in 23.45% of E coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from dlarrhoelc suckling piglets in Serbia. © 2020 Polskie Towarzystwo Nauk Weterynaryjnych.",
publisher = "Polskie Towarzystwo Nauk Weterynaryjnych (Polish Society of Veterinary Sciences)",
journal = "Medycyna Weterynaryjna",
title = "Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia",
volume = "76",
number = "1",
pages = "41-45",
doi = "10.21521/mw.6326"
}

Žutić, J., Valčić, O., Milićević, V., Veljović, L., Kureljušić, J., Bacić, D.,& Jezdimirović, N.. (2020). Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna
Polskie Towarzystwo Nauk Weterynaryjnych (Polish Society of Veterinary Sciences)., 76(1), 41-45.
https://doi.org/10.21521/mw.6326

Žutić J, Valčić O, Milićević V, Veljović L, Kureljušić J, Bacić D, Jezdimirović N. Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia. in Medycyna Weterynaryjna. 2020;76(1):41-45.
doi:10.21521/mw.6326 .

Žutić, Jadranka, Valčić, Olivera, Milićević, Vesna, Veljović, Ljubiša, Kureljušić, Jasna, Bacić, Dragan, Jezdimirović, Nemanja, "Profiles of virulence genes in enterotoxigenic Escherichia coll Isolates from suckling piglets in Serbia" in Medycyna Weterynaryjna, 76, no. 1 (2020):41-45,
https://doi.org/10.21521/mw.6326 . .

TY  - JOUR
AU  - Nikšić, Aleksandar
AU  - Ječmenica, Jovana
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2181
AB  - Cilj ovog rada je bio da se utvrdi da li postoje promene u koncentraciji glukoze, beta-hidroksibutirata (BHB) i aktivnosti glutation-peroksidaze (GPx) u zavisnosti od telesne kondicije kod junica. Junice su na osnovu telesne kondicije (TK) svrstane u dve grupe: 1. TK=3,75 i 2. TK ≥4,0. U ogledu je korišćena puna krv 23 junice uzeta 1-10 dan nakon partusa. Koncentracije glukoze i BHB određivane su odmah po uzorkovanju krvi, a aktivnost GPX određivana je nakon 24 sata. Junice sa većom telesnom kondicijom imale su statistički značajno veću prosečnu koncentraciju BHB i aktivnost GPX. Prosečna koncentracija glukoze u obe grupe se nije razlikovala.
AB  - The aim of this study was to establish if there are differences in glucose and ß-hydroxybutyrate (BHB) concentrations as well as glutathione peroxidase (GPx) activity related to the body condition in heifers. Based on the body condition (BC), heifers were divided into two groups: 1. BC=3.75 and 2. BC≥4.0. Whole blood samples from 23 heifers, taken between day 1 and day 10 after parturition, were used in the study. Glucose and BHB concentrations were determined immediately after sampling, while GPx activity was determined 24 hours after that.  Heifers with higher body condition had significantly higher average BHB concentration and GPx activity. Average glucose concentrations did not differ between groups.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Uticaj telesne kondicije junica na koncentraciju glukoze, beta-hidroksibutirata i aktivnost glutation-peroksidaze
T1  - The effect of body condition score of heifers on glucose and B-hydroxybutyrate concentrations and gluthatione peroxidase activity
VL  - 19
IS  - 2
SP  - 251
EP  - 264
DO  - 10.7251/VETJEN1902258N
ER  - 

@article{
author = "Nikšić, Aleksandar and Ječmenica, Jovana and Valčić, Olivera and Milanović, Svetlana",
year = "2019",
abstract = "Cilj ovog rada je bio da se utvrdi da li postoje promene u koncentraciji glukoze, beta-hidroksibutirata (BHB) i aktivnosti glutation-peroksidaze (GPx) u zavisnosti od telesne kondicije kod junica. Junice su na osnovu telesne kondicije (TK) svrstane u dve grupe: 1. TK=3,75 i 2. TK ≥4,0. U ogledu je korišćena puna krv 23 junice uzeta 1-10 dan nakon partusa. Koncentracije glukoze i BHB određivane su odmah po uzorkovanju krvi, a aktivnost GPX određivana je nakon 24 sata. Junice sa većom telesnom kondicijom imale su statistički značajno veću prosečnu koncentraciju BHB i aktivnost GPX. Prosečna koncentracija glukoze u obe grupe se nije razlikovala., The aim of this study was to establish if there are differences in glucose and ß-hydroxybutyrate (BHB) concentrations as well as glutathione peroxidase (GPx) activity related to the body condition in heifers. Based on the body condition (BC), heifers were divided into two groups: 1. BC=3.75 and 2. BC≥4.0. Whole blood samples from 23 heifers, taken between day 1 and day 10 after parturition, were used in the study. Glucose and BHB concentrations were determined immediately after sampling, while GPx activity was determined 24 hours after that.  Heifers with higher body condition had significantly higher average BHB concentration and GPx activity. Average glucose concentrations did not differ between groups.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Uticaj telesne kondicije junica na koncentraciju glukoze, beta-hidroksibutirata i aktivnost glutation-peroksidaze, The effect of body condition score of heifers on glucose and B-hydroxybutyrate concentrations and gluthatione peroxidase activity",
volume = "19",
number = "2",
pages = "251-264",
doi = "10.7251/VETJEN1902258N"
}

Nikšić, A., Ječmenica, J., Valčić, O.,& Milanović, S.. (2019). Uticaj telesne kondicije junica na koncentraciju glukoze, beta-hidroksibutirata i aktivnost glutation-peroksidaze. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 19(2), 251-264.
https://doi.org/10.7251/VETJEN1902258N

Nikšić A, Ječmenica J, Valčić O, Milanović S. Uticaj telesne kondicije junica na koncentraciju glukoze, beta-hidroksibutirata i aktivnost glutation-peroksidaze. in Veterinarski žurnal Republike Srpske. 2019;19(2):251-264.
doi:10.7251/VETJEN1902258N .

Nikšić, Aleksandar, Ječmenica, Jovana, Valčić, Olivera, Milanović, Svetlana, "Uticaj telesne kondicije junica na koncentraciju glukoze, beta-hidroksibutirata i aktivnost glutation-peroksidaze" in Veterinarski žurnal Republike Srpske, 19, no. 2 (2019):251-264,
https://doi.org/10.7251/VETJEN1902258N . .

TY  - CONF
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
AU  - Fratrić, Natalija
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3412
AB  - U radu su opisane osnovne grupe i biohemijske karakteristike organskih molekula koji su
od ključnog značaja za rast i razvoj novorođene prasadi. Među njima se posebno ističu laktoza,
koja ima ključnu ulogu u osmotskoj regulaciji količine izlučenog kolostruma i koja se sintetiše u
epitelnim ćelijama alveola mlečne žlezde. Zatim, masti koje su energetska komponenta čiji sastav
i količina zavise od niza faktora kao što su ishrana krmače i njen genetski potencijal. Prasad se
rađa agamaglobulinemična i neophodno je da u prvim časovima života posisaju kolostrum u
kojem su prisutna maternalna antitela (IgG) kako bi stekla imunitet neophodan za preživljavanje.
Pored navedenih sastojaka u kolostrumu se nalaze i visoke koncentracije minerala,
mikroelemenata, vitamina, hormona i enzima. Poseban osvrt u radu je dat dinamičnoj promeni
sastava i količine komponenti kolostruma od trenutka kada počinje da se luči do perioda tranzicije
u mleko. Istovremeno, objašnjeni su osnovni mehanizmi sinteze molekula u mlečnoj žlezdi i
transfera molekula iz krvne plazme svinje u kolostrum, kao i faktori koji utiču na sintezu ključnih
sastojaka kolostruma.
PB  - Požarevac : Veterinarski specijalistički institut
C3  - 17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019
T1  - Biohemijske karakteristike kolostruma
SP  - 112
EP  - 121
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3412
ER  - 

@conference{
author = "Valčić, Olivera and Milanović, Svetlana and Fratrić, Natalija",
year = "2019",
abstract = "U radu su opisane osnovne grupe i biohemijske karakteristike organskih molekula koji su
od ključnog značaja za rast i razvoj novorođene prasadi. Među njima se posebno ističu laktoza,
koja ima ključnu ulogu u osmotskoj regulaciji količine izlučenog kolostruma i koja se sintetiše u
epitelnim ćelijama alveola mlečne žlezde. Zatim, masti koje su energetska komponenta čiji sastav
i količina zavise od niza faktora kao što su ishrana krmače i njen genetski potencijal. Prasad se
rađa agamaglobulinemična i neophodno je da u prvim časovima života posisaju kolostrum u
kojem su prisutna maternalna antitela (IgG) kako bi stekla imunitet neophodan za preživljavanje.
Pored navedenih sastojaka u kolostrumu se nalaze i visoke koncentracije minerala,
mikroelemenata, vitamina, hormona i enzima. Poseban osvrt u radu je dat dinamičnoj promeni
sastava i količine komponenti kolostruma od trenutka kada počinje da se luči do perioda tranzicije
u mleko. Istovremeno, objašnjeni su osnovni mehanizmi sinteze molekula u mlečnoj žlezdi i
transfera molekula iz krvne plazme svinje u kolostrum, kao i faktori koji utiču na sintezu ključnih
sastojaka kolostruma.",
publisher = "Požarevac : Veterinarski specijalistički institut",
journal = "17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019",
title = "Biohemijske karakteristike kolostruma",
pages = "112-121",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3412"
}

Valčić, O., Milanović, S.,& Fratrić, N.. (2019). Biohemijske karakteristike kolostruma. in 17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019
Požarevac : Veterinarski specijalistički institut., 112-121.
https://hdl.handle.net/21.15107/rcub_veterinar_3412

Valčić O, Milanović S, Fratrić N. Biohemijske karakteristike kolostruma. in 17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019. 2019;:112-121.
https://hdl.handle.net/21.15107/rcub_veterinar_3412 .

Valčić, Olivera, Milanović, Svetlana, Fratrić, Natalija, "Biohemijske karakteristike kolostruma" in 17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019 (2019):112-121,
https://hdl.handle.net/21.15107/rcub_veterinar_3412 .

TY  - CONF
AU  - Žutić, Jadranka
AU  - Valčić, Olivera
AU  - Kureljušić, Branislav
AU  - Jakić-Dimić, Dobrila
AU  - Kureljušić, Jasna
AU  - Jezdimirović, Nemanja
AU  - Zdravković, Nemanja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3411
AB  - Respiratory diseases represent a significant problem in pig
farming as they can cause significant economic losses all over the World. One of
the most common causes of infection is Mycoplasma hyopneumoniae (M.
hyopneumoniae) either as the primary cause of enzootic pneumonia or as one of the
causative agents of Porcine Respiratory Disease Complex (PRCD). In our study we
presented the presence of antibodies against M. hyopneumoniae in 94 blood sera of
gilts and 84 blood sera of sows, originated from 4 commercial pig farms. For this
purpose, the indirect ELISA test was used. Seroconversion was established in 11
(11.70%) sera of gilts and in 15 (17.85%) sera of sows. The percentage of positive
sera differed among the farms, ranging in gilts from 0- 28.57%, and in sows 9.09-
36.84%. The results of the study have shown that infection with M. hyopneumoniae
is present in gilts on three and in sows on all four examined farms. Successful
control of M. hyopneumoniae depends on the efficient prevention of the transfer of
the infective agent. Good results can be achieved using strict implementation of
reliable serological methods. The serological control of gilts prior to fertilization is
of extreme importance, as it can prevent spreading of the disease within the herd.
PB  - Belgrade : Institute for Animal Husbandry
C3  - 12th International Symposium Modern trends in Livestock Production, Belgrade, 9 - 11 October 2019
T1  - Seroprevalence to Mycoplasma hyopneumoniae in gilts and sows
SP  - 533
EP  - 540
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3411
ER  - 

@conference{
author = "Žutić, Jadranka and Valčić, Olivera and Kureljušić, Branislav and Jakić-Dimić, Dobrila and Kureljušić, Jasna and Jezdimirović, Nemanja and Zdravković, Nemanja",
year = "2019",
abstract = "Respiratory diseases represent a significant problem in pig
farming as they can cause significant economic losses all over the World. One of
the most common causes of infection is Mycoplasma hyopneumoniae (M.
hyopneumoniae) either as the primary cause of enzootic pneumonia or as one of the
causative agents of Porcine Respiratory Disease Complex (PRCD). In our study we
presented the presence of antibodies against M. hyopneumoniae in 94 blood sera of
gilts and 84 blood sera of sows, originated from 4 commercial pig farms. For this
purpose, the indirect ELISA test was used. Seroconversion was established in 11
(11.70%) sera of gilts and in 15 (17.85%) sera of sows. The percentage of positive
sera differed among the farms, ranging in gilts from 0- 28.57%, and in sows 9.09-
36.84%. The results of the study have shown that infection with M. hyopneumoniae
is present in gilts on three and in sows on all four examined farms. Successful
control of M. hyopneumoniae depends on the efficient prevention of the transfer of
the infective agent. Good results can be achieved using strict implementation of
reliable serological methods. The serological control of gilts prior to fertilization is
of extreme importance, as it can prevent spreading of the disease within the herd.",
publisher = "Belgrade : Institute for Animal Husbandry",
journal = "12th International Symposium Modern trends in Livestock Production, Belgrade, 9 - 11 October 2019",
title = "Seroprevalence to Mycoplasma hyopneumoniae in gilts and sows",
pages = "533-540",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3411"
}

Žutić, J., Valčić, O., Kureljušić, B., Jakić-Dimić, D., Kureljušić, J., Jezdimirović, N.,& Zdravković, N.. (2019). Seroprevalence to Mycoplasma hyopneumoniae in gilts and sows. in 12th International Symposium Modern trends in Livestock Production, Belgrade, 9 - 11 October 2019
Belgrade : Institute for Animal Husbandry., 533-540.
https://hdl.handle.net/21.15107/rcub_veterinar_3411

Žutić J, Valčić O, Kureljušić B, Jakić-Dimić D, Kureljušić J, Jezdimirović N, Zdravković N. Seroprevalence to Mycoplasma hyopneumoniae in gilts and sows. in 12th International Symposium Modern trends in Livestock Production, Belgrade, 9 - 11 October 2019. 2019;:533-540.
https://hdl.handle.net/21.15107/rcub_veterinar_3411 .

Žutić, Jadranka, Valčić, Olivera, Kureljušić, Branislav, Jakić-Dimić, Dobrila, Kureljušić, Jasna, Jezdimirović, Nemanja, Zdravković, Nemanja, "Seroprevalence to Mycoplasma hyopneumoniae in gilts and sows" in 12th International Symposium Modern trends in Livestock Production, Belgrade, 9 - 11 October 2019 (2019):533-540,
https://hdl.handle.net/21.15107/rcub_veterinar_3411 .

TY  - JOUR
AU  - Bošnjaković, Dušan
AU  - Petrović, Aleksandra
AU  - Valčić, Olivera
AU  - Jovanović, Ivan B.
AU  - Milanović, Svetlana
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2164
AB  - Cilj rada je bio da se utvrdi stabilnost aktivnosti glutation peroksidaza 1 i 3 tokom skladištenja na temperaturi +4°C i -18°C. Krv je uzeta od osam ovaca, a aktivnost enzima je određivana u plazmi (GPx3) i eritrocitima (GPx1) prvog, trećeg, petog i sedmog dana u uzorcima čuvanim na +4°C i nakon jednog i tri meseca u uzorcima čuvanim na -18°C. Aktivnost GPx3 je tokom skladištenja na ovim temperaturama statistički značajno opala dok je aktivnost GPx1 ostala ista nakon tri meseca čuvanja na temperaturi -18°C. Rezultati ovog rada ukazuju na to da se GPx3 mora određivati u svežim uzorcima plazme ovaca, dok se aktivnost GPx1 eritrocita ovaca može određivati i nakon 3 meseca čuvanja na temperaturi -18°C.
AB  - The aim of the study was to determine the stability of the activity of glutathione peroxidases 1 and 3 during storage at +4°C and -18°C. Blood samples were taken from eight sheep and the activity of the enzyme was determined in the plasma (GPx3) and erythrocytes (GPx1) on the first, third, fifth and seventh day in samples stored at +4°C and after one and three months in samples stored at -18°C. GPx3 activity decreased significantly during storage at both temperatures, while GPx1 remained steady even after three months of storage at -18°C. Obtained results indicate that GPx3 activity has to be determined in fresh sheep plasma samples, while the activity of sheep erythrocyte GPx1 can be determined even after 3 months of storage at -18°C.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Uticaj različitih temperatura skladištenja na aktivnost glutation peroksidaza eritrocita i plazme ovaca
T1  - The effects of different storing temperatures on the activity of sheep blood and plasma glutathione peroxidase
VL  - 18
IS  - 1
SP  - 38
EP  - 53
DO  - 10.7251/VETJ1801038B
ER  - 

@article{
author = "Bošnjaković, Dušan and Petrović, Aleksandra and Valčić, Olivera and Jovanović, Ivan B. and Milanović, Svetlana",
year = "2018",
abstract = "Cilj rada je bio da se utvrdi stabilnost aktivnosti glutation peroksidaza 1 i 3 tokom skladištenja na temperaturi +4°C i -18°C. Krv je uzeta od osam ovaca, a aktivnost enzima je određivana u plazmi (GPx3) i eritrocitima (GPx1) prvog, trećeg, petog i sedmog dana u uzorcima čuvanim na +4°C i nakon jednog i tri meseca u uzorcima čuvanim na -18°C. Aktivnost GPx3 je tokom skladištenja na ovim temperaturama statistički značajno opala dok je aktivnost GPx1 ostala ista nakon tri meseca čuvanja na temperaturi -18°C. Rezultati ovog rada ukazuju na to da se GPx3 mora određivati u svežim uzorcima plazme ovaca, dok se aktivnost GPx1 eritrocita ovaca može određivati i nakon 3 meseca čuvanja na temperaturi -18°C., The aim of the study was to determine the stability of the activity of glutathione peroxidases 1 and 3 during storage at +4°C and -18°C. Blood samples were taken from eight sheep and the activity of the enzyme was determined in the plasma (GPx3) and erythrocytes (GPx1) on the first, third, fifth and seventh day in samples stored at +4°C and after one and three months in samples stored at -18°C. GPx3 activity decreased significantly during storage at both temperatures, while GPx1 remained steady even after three months of storage at -18°C. Obtained results indicate that GPx3 activity has to be determined in fresh sheep plasma samples, while the activity of sheep erythrocyte GPx1 can be determined even after 3 months of storage at -18°C.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Uticaj različitih temperatura skladištenja na aktivnost glutation peroksidaza eritrocita i plazme ovaca, The effects of different storing temperatures on the activity of sheep blood and plasma glutathione peroxidase",
volume = "18",
number = "1",
pages = "38-53",
doi = "10.7251/VETJ1801038B"
}

Bošnjaković, D., Petrović, A., Valčić, O., Jovanović, I. B.,& Milanović, S.. (2018). Uticaj različitih temperatura skladištenja na aktivnost glutation peroksidaza eritrocita i plazme ovaca. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 18(1), 38-53.
https://doi.org/10.7251/VETJ1801038B

Bošnjaković D, Petrović A, Valčić O, Jovanović IB, Milanović S. Uticaj različitih temperatura skladištenja na aktivnost glutation peroksidaza eritrocita i plazme ovaca. in Veterinarski žurnal Republike Srpske. 2018;18(1):38-53.
doi:10.7251/VETJ1801038B .

Bošnjaković, Dušan, Petrović, Aleksandra, Valčić, Olivera, Jovanović, Ivan B., Milanović, Svetlana, "Uticaj različitih temperatura skladištenja na aktivnost glutation peroksidaza eritrocita i plazme ovaca" in Veterinarski žurnal Republike Srpske, 18, no. 1 (2018):38-53,
https://doi.org/10.7251/VETJ1801038B . .

TY  - CONF
AU  - Milanović, Svetlana
AU  - Valčić, Olivera
AU  - Jovanović, Ivan
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3441
AB  - Komunikacija među živim svetom je vrlo kompleksan proces i može se odvijati na nekoliko načina. Najzastupljeniji oblici komunikacije među životinjama su vizuelna, auditivna i hemijska komunikacija. Kod ptica se smatra da
su najzastupljeniji vizuelni vidovi komunikacije, pa zatim auditivni. Do skoro
se vrlo malo pridavalo značaju proučavanja komunikacije kod ptica pomoću
hemijskih materija (mirisa i feromona) zbog slabo razvijenog olfaktornog bulbusa kod ovih kičmenjaka, kao i odsustva vomeronazalnog organa. Međutim,
novija istraživanja ukazuju na postojanje specifičnih receptora za određene hemijske materije i kod ptica. Kod nekih vrsta ptica, produkt uropigealne žlezde
sadrži materije koje mogu imati odlike feromona i koji nose informaciju o vrsti,
polu, identitetu, populaciji i poreklu. Kod sisara je ovaj vid komunikacije veoma
zastupljen, a kako su neki feromoni već izolovani i sintetski se proizvode, uveliko se koriste i u farmskoj proizvodnji. Zbog toga smo smatrali da je korisno
razmotriti razne mogućnosti primene feromona i u živinarskoj proizvodnji.
PB  - Beograd : Ciiip Živinarstvo
C3  - XXVII Savetovanje živinara, Tara, 2018
T1  - Moguća primena feromona u živinarskoj proizvodnji
SP  - 37
EP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3441
ER  - 

@conference{
author = "Milanović, Svetlana and Valčić, Olivera and Jovanović, Ivan",
year = "2018",
abstract = "Komunikacija među živim svetom je vrlo kompleksan proces i može se odvijati na nekoliko načina. Najzastupljeniji oblici komunikacije među životinjama su vizuelna, auditivna i hemijska komunikacija. Kod ptica se smatra da
su najzastupljeniji vizuelni vidovi komunikacije, pa zatim auditivni. Do skoro
se vrlo malo pridavalo značaju proučavanja komunikacije kod ptica pomoću
hemijskih materija (mirisa i feromona) zbog slabo razvijenog olfaktornog bulbusa kod ovih kičmenjaka, kao i odsustva vomeronazalnog organa. Međutim,
novija istraživanja ukazuju na postojanje specifičnih receptora za određene hemijske materije i kod ptica. Kod nekih vrsta ptica, produkt uropigealne žlezde
sadrži materije koje mogu imati odlike feromona i koji nose informaciju o vrsti,
polu, identitetu, populaciji i poreklu. Kod sisara je ovaj vid komunikacije veoma
zastupljen, a kako su neki feromoni već izolovani i sintetski se proizvode, uveliko se koriste i u farmskoj proizvodnji. Zbog toga smo smatrali da je korisno
razmotriti razne mogućnosti primene feromona i u živinarskoj proizvodnji.",
publisher = "Beograd : Ciiip Živinarstvo",
journal = "XXVII Savetovanje živinara, Tara, 2018",
title = "Moguća primena feromona u živinarskoj proizvodnji",
pages = "37-45",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3441"
}

Milanović, S., Valčić, O.,& Jovanović, I.. (2018). Moguća primena feromona u živinarskoj proizvodnji. in XXVII Savetovanje živinara, Tara, 2018
Beograd : Ciiip Živinarstvo., 37-45.
https://hdl.handle.net/21.15107/rcub_veterinar_3441

Milanović S, Valčić O, Jovanović I. Moguća primena feromona u živinarskoj proizvodnji. in XXVII Savetovanje živinara, Tara, 2018. 2018;:37-45.
https://hdl.handle.net/21.15107/rcub_veterinar_3441 .

Milanović, Svetlana, Valčić, Olivera, Jovanović, Ivan, "Moguća primena feromona u živinarskoj proizvodnji" in XXVII Savetovanje živinara, Tara, 2018 (2018):37-45,
https://hdl.handle.net/21.15107/rcub_veterinar_3441 .

TY  - JOUR
AU  - Bjelica, Jelena
AU  - Jovanović, Ivan B.
AU  - Ilić, Vesna
AU  - Valčić, Olivera
AU  - Kirovski, Danijela
AU  - Gvozdić, Dragan
AU  - Milanović, Svetlana
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1535
AB  - Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual infl uence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium defi ciency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU-group; selenium adequate, PTU+ group; selenium defi cient, PTU-group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 mg/L) in drinking water. After 3 weeks, thyroxine (T-4), triiodothyronine (T-3), and thyroid stimulating hormone (TSH) were determined. The animals having intermediate concentrations of T-3 (1.56-1.69 nmol/L) and T 4 (41-50 nmol/L) were excluded from further analysis. Thus, PTU+ groups included hypothyroid animals (T-3 <= 1.55 nmol/L; T-4 <= 40 nmol/L), while PTU-groups included euthyroid rats (T-3 <= 1.70 nmol/L; T-4 <= 50 nmol/L). Both groups of selenium defi cient rats had, when compared to the control group, a signifi cantly lower activity of glutathione peroxidase GPx1 and GPx3. Neither selenium defi ciency nor PTU infl uenced the adherence of peritoneal macrophages. Selenium defi ciency signifi cantly decreased the peroxide synthesis in macrophages and signifi cantly increased the titer of anti-SRBC IgM. Hypotyroidism alone or in combination with selenium defi ciency had no infl uence on these parameters.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats
VL  - 67
IS  - 3
SP  - 340
EP  - 355
DO  - 10.1515/acve-2017-0028
ER  - 

@article{
author = "Bjelica, Jelena and Jovanović, Ivan B. and Ilić, Vesna and Valčić, Olivera and Kirovski, Danijela and Gvozdić, Dragan and Milanović, Svetlana",
year = "2017",
abstract = "Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual infl uence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium defi ciency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU-group; selenium adequate, PTU+ group; selenium defi cient, PTU-group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 mg/L) in drinking water. After 3 weeks, thyroxine (T-4), triiodothyronine (T-3), and thyroid stimulating hormone (TSH) were determined. The animals having intermediate concentrations of T-3 (1.56-1.69 nmol/L) and T 4 (41-50 nmol/L) were excluded from further analysis. Thus, PTU+ groups included hypothyroid animals (T-3 <= 1.55 nmol/L; T-4 <= 40 nmol/L), while PTU-groups included euthyroid rats (T-3 <= 1.70 nmol/L; T-4 <= 50 nmol/L). Both groups of selenium defi cient rats had, when compared to the control group, a signifi cantly lower activity of glutathione peroxidase GPx1 and GPx3. Neither selenium defi ciency nor PTU infl uenced the adherence of peritoneal macrophages. Selenium defi ciency signifi cantly decreased the peroxide synthesis in macrophages and signifi cantly increased the titer of anti-SRBC IgM. Hypotyroidism alone or in combination with selenium defi ciency had no infl uence on these parameters.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats",
volume = "67",
number = "3",
pages = "340-355",
doi = "10.1515/acve-2017-0028"
}

Bjelica, J., Jovanović, I. B., Ilić, V., Valčić, O., Kirovski, D., Gvozdić, D.,& Milanović, S.. (2017). Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(3), 340-355.
https://doi.org/10.1515/acve-2017-0028

Bjelica J, Jovanović IB, Ilić V, Valčić O, Kirovski D, Gvozdić D, Milanović S. Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats. in Acta Veterinaria-Beograd. 2017;67(3):340-355.
doi:10.1515/acve-2017-0028 .

Bjelica, Jelena, Jovanović, Ivan B., Ilić, Vesna, Valčić, Olivera, Kirovski, Danijela, Gvozdić, Dragan, Milanović, Svetlana, "Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats" in Acta Veterinaria-Beograd, 67, no. 3 (2017):340-355,
https://doi.org/10.1515/acve-2017-0028 . .

TY  - JOUR
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2159
AB  - Актуелни трендови употребе биљних производа у заштити здравља људи и
животиња довели су до широке примене природних полифенола присутних у
биљкама, као што су зелени чај, боровница, аронија, итд. На основу досадашњих
испитивања настанка оксидативног стреса као и начина превенције, можемо
закључити да су грожђе, семенке грожђа, као и комина показали одређене протективне ефекте. In vivo студије ефикасности полифенола су изузетно комплексне и до сада су дале контроверзне резултате. У овде представљеном раду дат је
преглед до сада постигнутих сазнања ефеката употребе полифенола грожђа у
заштити од оксидативног стреса домаћих животиња.
AB  - Current trends on the use of plant products in the protection of human and animal
health have resulted in the widespread use of natural polyphenols present in plants
such as green tea, chokeberries, blackberries, etc. According to a wide array of trials
on oxidative stress and its prevention it can be concluded that grapes, grapeseeds and pomace have some protective effects. In vivo polyphenol efficacy studies are extremely
complex and up to date have raised a number of controversial results. In the here
presented paper we have offered a review on the knowledge on grape polyphenols in
oxidative stress protection of domestic animals.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Polifenoli grožđa u zaštiti domaćih životinja od oksidativnog stresa
T1  - Grape polyphenols in the protection of domestic animals from oxidative stress
VL  - 17
IS  - 2
SP  - 182
EP  - 191
DO  - 10.7251/VETJ1702182V
ER  - 

@article{
author = "Valčić, Olivera and Milanović, Svetlana",
year = "2017",
abstract = "Актуелни трендови употребе биљних производа у заштити здравља људи и
животиња довели су до широке примене природних полифенола присутних у
биљкама, као што су зелени чај, боровница, аронија, итд. На основу досадашњих
испитивања настанка оксидативног стреса као и начина превенције, можемо
закључити да су грожђе, семенке грожђа, као и комина показали одређене протективне ефекте. In vivo студије ефикасности полифенола су изузетно комплексне и до сада су дале контроверзне резултате. У овде представљеном раду дат је
преглед до сада постигнутих сазнања ефеката употребе полифенола грожђа у
заштити од оксидативног стреса домаћих животиња., Current trends on the use of plant products in the protection of human and animal
health have resulted in the widespread use of natural polyphenols present in plants
such as green tea, chokeberries, blackberries, etc. According to a wide array of trials
on oxidative stress and its prevention it can be concluded that grapes, grapeseeds and pomace have some protective effects. In vivo polyphenol efficacy studies are extremely
complex and up to date have raised a number of controversial results. In the here
presented paper we have offered a review on the knowledge on grape polyphenols in
oxidative stress protection of domestic animals.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Polifenoli grožđa u zaštiti domaćih životinja od oksidativnog stresa, Grape polyphenols in the protection of domestic animals from oxidative stress",
volume = "17",
number = "2",
pages = "182-191",
doi = "10.7251/VETJ1702182V"
}

Valčić, O.,& Milanović, S.. (2017). Polifenoli grožđa u zaštiti domaćih životinja od oksidativnog stresa. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 17(2), 182-191.
https://doi.org/10.7251/VETJ1702182V

Valčić O, Milanović S. Polifenoli grožđa u zaštiti domaćih životinja od oksidativnog stresa. in Veterinarski žurnal Republike Srpske. 2017;17(2):182-191.
doi:10.7251/VETJ1702182V .

Valčić, Olivera, Milanović, Svetlana, "Polifenoli grožđa u zaštiti domaćih životinja od oksidativnog stresa" in Veterinarski žurnal Republike Srpske, 17, no. 2 (2017):182-191,
https://doi.org/10.7251/VETJ1702182V . .

TY  - CONF
AU  - Milanović, Svetlana
AU  - Valčić, Olivera
AU  - Jovanović, Ivan
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3440
AB  - Sve veći zahtevi tržišta za mesom koje sadrži što manje masti i holesterola
doveli su do značajnog porasta proizvodnje živinskog mesa. Ukupna proizvodnja živinskog mesa bila je 2001. godine 70 miliona tona što je osam puta
više od proizvodnje u 1961. godini, a uz godišnju stopu porasta od 2,8%, 2015.
godine je dostigla oko 100 miliona tona. Sa druge strane, pred proizvođače
se postavlja zadatak da za što kraće vreme proizvedu što više kvalitetnog i
zdravstveno-higijenski ispravnog proizvoda. Ovaj zadatak nameće upotrebu
kvalitetnih hraniva kao i dodataka koji će u potpunosti zadovoljiti osnovne i
proizvodne potrebe životinja u uzgoju, maksimalno iskorišćavajući genetske
kapacitete jedinki. Mikroelementi su jedan od ključnih faktora potrebnih za
pravilno funkcionisanje organizma. U intenzivnoj proizvodnji njihova je upotreba obavezna, a sa sve većim potrebama organizma, paralelno se razvijaju
različiti oblici mikroelemenata za koje se smatra da bi mogli imati veću bioiskoristivost. Mikroelementima pripadaju elementi koji su u organizmu zastupljeni sa manje od 100 mg/kg telesne mase. Njihova funkcija u organizmu
vezana je za funkciju proteina, enzima ili kofaktora u čiji sastav ulaze. U ovom
radu biće prikazane biološke funkcije nekih od njih, sa posebnim osvrtom na
bioiskoristivost različith neorganskih i organskih oblika gvožđa, mangana,
bakra, cinka i selena.
PB  - Beograd : Ciiip Živinarstvo
C3  - XXVI Savetovanje živinara, Tara, 2017
T1  - Uloga i značaj mikroelemenata u savremenom živinarstvu
SP  - 36
EP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3440
ER  - 

@conference{
author = "Milanović, Svetlana and Valčić, Olivera and Jovanović, Ivan",
year = "2017",
abstract = "Sve veći zahtevi tržišta za mesom koje sadrži što manje masti i holesterola
doveli su do značajnog porasta proizvodnje živinskog mesa. Ukupna proizvodnja živinskog mesa bila je 2001. godine 70 miliona tona što je osam puta
više od proizvodnje u 1961. godini, a uz godišnju stopu porasta od 2,8%, 2015.
godine je dostigla oko 100 miliona tona. Sa druge strane, pred proizvođače
se postavlja zadatak da za što kraće vreme proizvedu što više kvalitetnog i
zdravstveno-higijenski ispravnog proizvoda. Ovaj zadatak nameće upotrebu
kvalitetnih hraniva kao i dodataka koji će u potpunosti zadovoljiti osnovne i
proizvodne potrebe životinja u uzgoju, maksimalno iskorišćavajući genetske
kapacitete jedinki. Mikroelementi su jedan od ključnih faktora potrebnih za
pravilno funkcionisanje organizma. U intenzivnoj proizvodnji njihova je upotreba obavezna, a sa sve većim potrebama organizma, paralelno se razvijaju
različiti oblici mikroelemenata za koje se smatra da bi mogli imati veću bioiskoristivost. Mikroelementima pripadaju elementi koji su u organizmu zastupljeni sa manje od 100 mg/kg telesne mase. Njihova funkcija u organizmu
vezana je za funkciju proteina, enzima ili kofaktora u čiji sastav ulaze. U ovom
radu biće prikazane biološke funkcije nekih od njih, sa posebnim osvrtom na
bioiskoristivost različith neorganskih i organskih oblika gvožđa, mangana,
bakra, cinka i selena.",
publisher = "Beograd : Ciiip Živinarstvo",
journal = "XXVI Savetovanje živinara, Tara, 2017",
title = "Uloga i značaj mikroelemenata u savremenom živinarstvu",
pages = "36-45",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3440"
}

Milanović, S., Valčić, O.,& Jovanović, I.. (2017). Uloga i značaj mikroelemenata u savremenom živinarstvu. in XXVI Savetovanje živinara, Tara, 2017
Beograd : Ciiip Živinarstvo., 36-45.
https://hdl.handle.net/21.15107/rcub_veterinar_3440

Milanović S, Valčić O, Jovanović I. Uloga i značaj mikroelemenata u savremenom živinarstvu. in XXVI Savetovanje živinara, Tara, 2017. 2017;:36-45.
https://hdl.handle.net/21.15107/rcub_veterinar_3440 .

Milanović, Svetlana, Valčić, Olivera, Jovanović, Ivan, "Uloga i značaj mikroelemenata u savremenom živinarstvu" in XXVI Savetovanje živinara, Tara, 2017 (2017):36-45,
https://hdl.handle.net/21.15107/rcub_veterinar_3440 .

TY  - JOUR
AU  - Stojić, Milica
AU  - Fratrić, Natalija
AU  - Kovacić, Marijana
AU  - Ilić, Vesna
AU  - Gvozdić, Dragan
AU  - Savić, Olivera
AU  - Doković, Radojica
AU  - Valčić, Olivera
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1519
AB  - Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P<0.001 and P<0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin<10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer
VL  - 67
IS  - 4
SP  - 508
EP  - 524
DO  - 10.1515/acve-2017-0041
ER  - 

@article{
author = "Stojić, Milica and Fratrić, Natalija and Kovacić, Marijana and Ilić, Vesna and Gvozdić, Dragan and Savić, Olivera and Doković, Radojica and Valčić, Olivera",
year = "2017",
abstract = "Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P<0.001 and P<0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin<10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer",
volume = "67",
number = "4",
pages = "508-524",
doi = "10.1515/acve-2017-0041"
}

Stojić, M., Fratrić, N., Kovacić, M., Ilić, V., Gvozdić, D., Savić, O., Doković, R.,& Valčić, O.. (2017). Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(4), 508-524.
https://doi.org/10.1515/acve-2017-0041

Stojić M, Fratrić N, Kovacić M, Ilić V, Gvozdić D, Savić O, Doković R, Valčić O. Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. in Acta Veterinaria-Beograd. 2017;67(4):508-524.
doi:10.1515/acve-2017-0041 .

Stojić, Milica, Fratrić, Natalija, Kovacić, Marijana, Ilić, Vesna, Gvozdić, Dragan, Savić, Olivera, Doković, Radojica, Valčić, Olivera, "Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer" in Acta Veterinaria-Beograd, 67, no. 4 (2017):508-524,
https://doi.org/10.1515/acve-2017-0041 . .

TY  - JOUR
AU  - Buac, Marijana
AU  - Mojsilović, Slavko
AU  - Mišić, Dušan
AU  - Vuković, Dejan
AU  - Savić, Olivera
AU  - Valčić, Olivera
AU  - Marković, Dragana
AU  - Gvozdić, Dragan
AU  - Ilić, Vesna
AU  - Fratrić, Natalija
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1403
AB  - In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes viability. The strongest effect of inhibition of cows granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation
VL  - 106
SP  - 135
EP  - 142
DO  - 10.1016/j.rvsc.2016.04.002
ER  - 

@article{
author = "Buac, Marijana and Mojsilović, Slavko and Mišić, Dušan and Vuković, Dejan and Savić, Olivera and Valčić, Olivera and Marković, Dragana and Gvozdić, Dragan and Ilić, Vesna and Fratrić, Natalija",
year = "2016",
abstract = "In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes viability. The strongest effect of inhibition of cows granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation",
volume = "106",
pages = "135-142",
doi = "10.1016/j.rvsc.2016.04.002"
}

Buac, M., Mojsilović, S., Mišić, D., Vuković, D., Savić, O., Valčić, O., Marković, D., Gvozdić, D., Ilić, V.,& Fratrić, N.. (2016). Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 106, 135-142.
https://doi.org/10.1016/j.rvsc.2016.04.002

Buac M, Mojsilović S, Mišić D, Vuković D, Savić O, Valčić O, Marković D, Gvozdić D, Ilić V, Fratrić N. Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. in Research in Veterinary Science. 2016;106:135-142.
doi:10.1016/j.rvsc.2016.04.002 .

Buac, Marijana, Mojsilović, Slavko, Mišić, Dušan, Vuković, Dejan, Savić, Olivera, Valčić, Olivera, Marković, Dragana, Gvozdić, Dragan, Ilić, Vesna, Fratrić, Natalija, "Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation" in Research in Veterinary Science, 106 (2016):135-142,
https://doi.org/10.1016/j.rvsc.2016.04.002 . .