TY  - JOUR
AU  - Milanović, Zorana
AU  - Ilić Božović, Anja
AU  - Bojić-Trbojević, Žanka
AU  - Hajduković, Ljiljana
AU  - Francuski Andrić, Jelena
AU  - Kovačević Filipović, Milica
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3631
AB  - Acute B. canis infection can lead to an acute phase reaction (APR) in dogs. The parasite invades red blood cells causing
anemia through immune-mediated hemolysis and possible erythropoietic suppression. A regenerative response of the
erythroid lineage during the babesiosis has not been described in extension. This research examines hematologic parameters
focusing on the absolute reticulocyte count and apolipoprotein A I (ApoA I) level on the day of admission and 14 days after
treatment with imidocarb-dipropionate in young (n=11) and adult (n=11) dogs naturally infected with B. canis. Metabolic
and inflammatory processes were characterized by analyzing protein and lipid profiles, as well as ApoA I at specified time
points. Automated analyzers were used to determine complete blood count and biochemical parameters, while ApoA I was
assessed using radioimmunoassay. The reticulocyte count was determined using a manual method by means of supravital
staining. Both young and adult dogs with acute B. canis infection showed non-regenerative anemia without difference.
Fourteen days after successful treatment with imidocarb-dipropionate, the anemia was corrected and a high reticulocyte
count was observed (p<0.05). This indicates that the erythroid regenerative response was efficient in young and adult dogs,
although vital signs, leukocyte count and triglyceride concentration suggest a more intense APR in young dogs. A decrease
in ApoA I in both groups 14 days after treatment (p<0.01) confirmed that this lipoprotein acts as a positive acute-phase
protein in acute B. canis infection in dogs, but further studies are needed to connect its role in erythroid lineage regeneration.
PB  - Saints Cyril and Methodius University of Skopje, Faculty of Veterinary Medicine
T2  - Macedonian Veterinary Review
T1  - Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs
VL  - 47
IS  - 1
DO  - 10.2478/macvetrev-2024-0011
ER  - 

@article{
author = "Milanović, Zorana and Ilić Božović, Anja and Bojić-Trbojević, Žanka and Hajduković, Ljiljana and Francuski Andrić, Jelena and Kovačević Filipović, Milica",
year = "2024",
abstract = "Acute B. canis infection can lead to an acute phase reaction (APR) in dogs. The parasite invades red blood cells causing
anemia through immune-mediated hemolysis and possible erythropoietic suppression. A regenerative response of the
erythroid lineage during the babesiosis has not been described in extension. This research examines hematologic parameters
focusing on the absolute reticulocyte count and apolipoprotein A I (ApoA I) level on the day of admission and 14 days after
treatment with imidocarb-dipropionate in young (n=11) and adult (n=11) dogs naturally infected with B. canis. Metabolic
and inflammatory processes were characterized by analyzing protein and lipid profiles, as well as ApoA I at specified time
points. Automated analyzers were used to determine complete blood count and biochemical parameters, while ApoA I was
assessed using radioimmunoassay. The reticulocyte count was determined using a manual method by means of supravital
staining. Both young and adult dogs with acute B. canis infection showed non-regenerative anemia without difference.
Fourteen days after successful treatment with imidocarb-dipropionate, the anemia was corrected and a high reticulocyte
count was observed (p<0.05). This indicates that the erythroid regenerative response was efficient in young and adult dogs,
although vital signs, leukocyte count and triglyceride concentration suggest a more intense APR in young dogs. A decrease
in ApoA I in both groups 14 days after treatment (p<0.01) confirmed that this lipoprotein acts as a positive acute-phase
protein in acute B. canis infection in dogs, but further studies are needed to connect its role in erythroid lineage regeneration.",
publisher = "Saints Cyril and Methodius University of Skopje, Faculty of Veterinary Medicine",
journal = "Macedonian Veterinary Review",
title = "Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs",
volume = "47",
number = "1",
doi = "10.2478/macvetrev-2024-0011"
}

Milanović, Z., Ilić Božović, A., Bojić-Trbojević, Ž., Hajduković, L., Francuski Andrić, J.,& Kovačević Filipović, M.. (2024). Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs. in Macedonian Veterinary Review
Saints Cyril and Methodius University of Skopje, Faculty of Veterinary Medicine., 47(1).
https://doi.org/10.2478/macvetrev-2024-0011

Milanović Z, Ilić Božović A, Bojić-Trbojević Ž, Hajduković L, Francuski Andrić J, Kovačević Filipović M. Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs. in Macedonian Veterinary Review. 2024;47(1).
doi:10.2478/macvetrev-2024-0011 .

Milanović, Zorana, Ilić Božović, Anja, Bojić-Trbojević, Žanka, Hajduković, Ljiljana, Francuski Andrić, Jelena, Kovačević Filipović, Milica, "Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs" in Macedonian Veterinary Review, 47, no. 1 (2024),
https://doi.org/10.2478/macvetrev-2024-0011 . .

TY  - JOUR
AU  - Ilić Božović, Anja
AU  - Đoković, Petar
AU  - Milanović, Zorana
AU  - Janjić, Filip
AU  - Spariosu, Kristina
AU  - Radonjić, Vladimir
AU  - Radaković, Milena
AU  - Magaš, Vladimir
AU  - Filipović, Dimitrije
AU  - Stanković, Sanja
AU  - Kovačević Filipović, Milica
AU  - Beletić, Andelo
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2934
AB  - Homocysteine (Hcy) was investigated as the biomarker of cardiac, renal, and gastrointestinal disorders
in dogs. Data about low Hcy concentrations in the systemic inflammatory response syndrome raised a hypothesis that
Hcy in dogs could be a negative acute-phase reactant. This survey compared Hcy concentrations, serum amyloid A
(SAA), and the routine laboratory parameters between healthy (HD, N=6) and dogs with inflammation of different
extent: mild (dirofilariosis (DIR), N=31), moderate (babesiosis (BAB), N=12), and severe (pyometra (PYO), N=8).
The BAB and PYO groups had lower Hcy er than HD. Also, the levels in the PYO group were below those in the
DIRO group. SAA had the inverse pattern. Across the groups, Hcy and SAA levels correlated negatively (ρ = -0.502,
P<0.001). Hcy and SAA correlated with the erythrocyte count, hematocrit, hemoglobin and mean cellular hemoglobin
concentrations, and neutrophil count, with correlations being positive for Hcy and negative for SAA. Among all dogs,
hemoglobin was the only independent predictor of Hcy concentration. Hcy levels in canine infections, decreased as
acute-phase reaction (APR) intensified. Also, they were related with the hematology changes accompanying the APR.
Further studies will establish the clinical potential of these alterations.
PB  - Hellenic Veterinary Medical Society
T2  - Journal of the Hellenic Veterinary Medical Society
T1  - Could homocysteine represent a negative acute phase reactant in canine infections-a pilot study?
VL  - 74
IS  - 1
SP  - 5463
EP  - 5470
DO  - 10.12681/jhvms.29529
ER  - 

@article{
author = "Ilić Božović, Anja and Đoković, Petar and Milanović, Zorana and Janjić, Filip and Spariosu, Kristina and Radonjić, Vladimir and Radaković, Milena and Magaš, Vladimir and Filipović, Dimitrije and Stanković, Sanja and Kovačević Filipović, Milica and Beletić, Andelo",
year = "2023",
abstract = "Homocysteine (Hcy) was investigated as the biomarker of cardiac, renal, and gastrointestinal disorders
in dogs. Data about low Hcy concentrations in the systemic inflammatory response syndrome raised a hypothesis that
Hcy in dogs could be a negative acute-phase reactant. This survey compared Hcy concentrations, serum amyloid A
(SAA), and the routine laboratory parameters between healthy (HD, N=6) and dogs with inflammation of different
extent: mild (dirofilariosis (DIR), N=31), moderate (babesiosis (BAB), N=12), and severe (pyometra (PYO), N=8).
The BAB and PYO groups had lower Hcy er than HD. Also, the levels in the PYO group were below those in the
DIRO group. SAA had the inverse pattern. Across the groups, Hcy and SAA levels correlated negatively (ρ = -0.502,
P<0.001). Hcy and SAA correlated with the erythrocyte count, hematocrit, hemoglobin and mean cellular hemoglobin
concentrations, and neutrophil count, with correlations being positive for Hcy and negative for SAA. Among all dogs,
hemoglobin was the only independent predictor of Hcy concentration. Hcy levels in canine infections, decreased as
acute-phase reaction (APR) intensified. Also, they were related with the hematology changes accompanying the APR.
Further studies will establish the clinical potential of these alterations.",
publisher = "Hellenic Veterinary Medical Society",
journal = "Journal of the Hellenic Veterinary Medical Society",
title = "Could homocysteine represent a negative acute phase reactant in canine infections-a pilot study?",
volume = "74",
number = "1",
pages = "5463-5470",
doi = "10.12681/jhvms.29529"
}

Ilić Božović, A., Đoković, P., Milanović, Z., Janjić, F., Spariosu, K., Radonjić, V., Radaković, M., Magaš, V., Filipović, D., Stanković, S., Kovačević Filipović, M.,& Beletić, A.. (2023). Could homocysteine represent a negative acute phase reactant in canine infections-a pilot study?. in Journal of the Hellenic Veterinary Medical Society
Hellenic Veterinary Medical Society., 74(1), 5463-5470.
https://doi.org/10.12681/jhvms.29529

Ilić Božović A, Đoković P, Milanović Z, Janjić F, Spariosu K, Radonjić V, Radaković M, Magaš V, Filipović D, Stanković S, Kovačević Filipović M, Beletić A. Could homocysteine represent a negative acute phase reactant in canine infections-a pilot study?. in Journal of the Hellenic Veterinary Medical Society. 2023;74(1):5463-5470.
doi:10.12681/jhvms.29529 .

Ilić Božović, Anja, Đoković, Petar, Milanović, Zorana, Janjić, Filip, Spariosu, Kristina, Radonjić, Vladimir, Radaković, Milena, Magaš, Vladimir, Filipović, Dimitrije, Stanković, Sanja, Kovačević Filipović, Milica, Beletić, Andelo, "Could homocysteine represent a negative acute phase reactant in canine infections-a pilot study?" in Journal of the Hellenic Veterinary Medical Society, 74, no. 1 (2023):5463-5470,
https://doi.org/10.12681/jhvms.29529 . .

TY  - JOUR
AU  - Milanović, Zorana
AU  - Beletić, Anđelo
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Andrić, Nenad
AU  - Božović, Anja Ilić
AU  - Spariosu, Kristina
AU  - Radaković, Milena
AU  - Ajtić, Jelena
AU  - Kovačević-Filipović, Milica
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1875
AB  - Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.
PB  - Elsevier Science Bv, Amsterdam
T2  - Veterinary Parasitology
T1  - Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis
VL  - 282
SP  - 109140
DO  - 10.1016/j.vetpar.2020.109140
ER  - 

@article{
author = "Milanović, Zorana and Beletić, Anđelo and Vekić, Jelena and Zeljković, Aleksandra and Andrić, Nenad and Božović, Anja Ilić and Spariosu, Kristina and Radaković, Milena and Ajtić, Jelena and Kovačević-Filipović, Milica",
year = "2020",
abstract = "Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Veterinary Parasitology",
title = "Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis",
volume = "282",
pages = "109140",
doi = "10.1016/j.vetpar.2020.109140"
}

Milanović, Z., Beletić, A., Vekić, J., Zeljković, A., Andrić, N., Božović, A. I., Spariosu, K., Radaković, M., Ajtić, J.,& Kovačević-Filipović, M.. (2020). Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis. in Veterinary Parasitology
Elsevier Science Bv, Amsterdam., 282, 109140.
https://doi.org/10.1016/j.vetpar.2020.109140

Milanović Z, Beletić A, Vekić J, Zeljković A, Andrić N, Božović AI, Spariosu K, Radaković M, Ajtić J, Kovačević-Filipović M. Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis. in Veterinary Parasitology. 2020;282:109140.
doi:10.1016/j.vetpar.2020.109140 .

Milanović, Zorana, Beletić, Anđelo, Vekić, Jelena, Zeljković, Aleksandra, Andrić, Nenad, Božović, Anja Ilić, Spariosu, Kristina, Radaković, Milena, Ajtić, Jelena, Kovačević-Filipović, Milica, "Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis" in Veterinary Parasitology, 282 (2020):109140,
https://doi.org/10.1016/j.vetpar.2020.109140 . .

TY  - JOUR
AU  - Janjić, Filip
AU  - Milanović, Zorana
AU  - Ilić Božović, Anja
AU  - Andrić, Nenad
AU  - Spariosu, Kristina
AU  - Ajtić, Jelena
AU  - Beletić, Anđelo
AU  - Kovačević-Filipović, Milica
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2214
AB  - Haematologic abnormalities possibly associated with exposure to vector-borne pathogens are rarely reported in clinically healthy outdoor dogs. Therefore, we analysed changes in the complete blood count (CBC) of clinically healthy outdoor dogs seroreactive to Anaplasma spp. and Babesia spp., with or without microfilariosis. Stray, shelter and hunting dogs, 81 in total, that were polymerase chain reaction negative for Anaplasma spp. and Babesia spp. were divided into groups according to their seroreactive status and results of a modified Knott’s test: seronegative to both Anaplasma spp. and Babesia spp. SN (N=26); seroreactive to A. phagocythophilum SR-A (N=12); seroreactive to B. canis, B. gibsoni and/or B. vogeli SR-B (N=25); and seroreactive to both of the pathogens SR-AB (N=8). These four groups were negative to microfilariosis, unlike the fifth group, seroreactive to either or to both of the pathogens and with microfilariosis SR-M (N=10). The frequencies of CBC alterations among all analysed dogs were: 0.35 – leucocytosis, 0.44 – granulocytosis, 0.28 – anaemia, 0.74 – microcytosis, 0.37 – increased mean cell haemoglobin concentration (MCHC) and 0.33 – thrombocytopenia. The frequency of alterations did not differ across the groups. An exception was the SR-M group wherein increased MCHC peaked with a frequency of 0.80, while in the 
other four groups, the frequency ranged between 0.10 and 0.50. Clinically healthy outdoor dogs have multiple CBC abnormalities, consistent with stress and low-level chronic inflammation, but not associated with a previous exposure to Anaplasma spp. or Babesia spp. The presence of microfilaria increases haemolysis in vitro.
AB  - Klinički zdravi psi koji borave na otvorenom i koji su izloženi patogenima koje prenose vektori mogu imati hematološke premećaje vezane za dejstvo tih patogena. Ipak, hematološke pretrage se retko izvode na toj populaciji pasa. Stoga je cilj našeg rada bio da utvrdimo promene u krvnoj slici zdravih pasa lutalica, pasa smeštenih u azilima i lovačkih pasa, seroreaktivnih na uzročnike bolesti iz rodova Anaplasma i Babesia, sa ili bez mikrofilarioze. U ovom radu je ispitana krvna slika 81 psa. U studiju su uključeni psi kod kojih lančanom reakcijom polimerizacije nije pokazano prisustvo DNK Anaplasma spp. i Babesia spp. Psi su prema serološkom statusu raspoređeni u sledeće grupe: seronegativni na Anaplasma spp. i Babesia spp. SN (N=26), seroreaktivni na A. phagocytophilum SR-A (N=12), seroreaktivni na B. canis, B. gibsoni i/ili B. vogeli SR-B (N=25) i seroreaktivni na oba navedena patogena SR-AB (N=8). Ove četiri grupe su bile negativne na mikrofilariozu, dok je peta grupa, seroreaktivna na neki ili oba patogena imala i mikrofilarije SR-M (N=10). Učestalost promena kompletne krvne slike među svim ispitivanim psima je bila sledeća: 0,35 za leukocitozu, 0,44 za granulocitozu, 0,28 za anemiju, 0,74 za mikrocitozu, 0,37 za povećanu prosečnu koncentraciju hemoglobina u eritrocitima i 0,33 za trombocitopeniju. Grupe se nisu razlikovale u pogledu učestalosti navedenih promena. Izuzetak je grupa SR-M u kojoj je učestalost povećanja vrednosti za prosečnu koncentraciju hemoglobina u eritrocitima iznosila 0,80. U ostale četiri grupe ta učestalost je iznosila između 0,10 i 0,50. Zaključak je da ispitivani psi imaju višestruke poremećaje krvne slike koji najverovatnije odražavaju stres i nizak stepen hronične inflamacije, ali ne i vezu sa prethodnim kontaktom sa Anaplasma spp. i Babesia spp. Prisustvo mikrofilarija izaziva hemolizu in vitro.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Hаematologic indices in clinically healthy outdoor dogs exposed to vector-borne pathogens
T1  - Hematološki parametri kod klinički zdravih pasa koji borave na otvorenom i izloženi su patogenima koje prenose vektori
VL  - 74
IS  - 2
SP  - 178
EP  - 186
DO  - 10.2298/VETGL191216005J
ER  - 

@article{
author = "Janjić, Filip and Milanović, Zorana and Ilić Božović, Anja and Andrić, Nenad and Spariosu, Kristina and Ajtić, Jelena and Beletić, Anđelo and Kovačević-Filipović, Milica",
year = "2020",
abstract = "Haematologic abnormalities possibly associated with exposure to vector-borne pathogens are rarely reported in clinically healthy outdoor dogs. Therefore, we analysed changes in the complete blood count (CBC) of clinically healthy outdoor dogs seroreactive to Anaplasma spp. and Babesia spp., with or without microfilariosis. Stray, shelter and hunting dogs, 81 in total, that were polymerase chain reaction negative for Anaplasma spp. and Babesia spp. were divided into groups according to their seroreactive status and results of a modified Knott’s test: seronegative to both Anaplasma spp. and Babesia spp. SN (N=26); seroreactive to A. phagocythophilum SR-A (N=12); seroreactive to B. canis, B. gibsoni and/or B. vogeli SR-B (N=25); and seroreactive to both of the pathogens SR-AB (N=8). These four groups were negative to microfilariosis, unlike the fifth group, seroreactive to either or to both of the pathogens and with microfilariosis SR-M (N=10). The frequencies of CBC alterations among all analysed dogs were: 0.35 – leucocytosis, 0.44 – granulocytosis, 0.28 – anaemia, 0.74 – microcytosis, 0.37 – increased mean cell haemoglobin concentration (MCHC) and 0.33 – thrombocytopenia. The frequency of alterations did not differ across the groups. An exception was the SR-M group wherein increased MCHC peaked with a frequency of 0.80, while in the 
other four groups, the frequency ranged between 0.10 and 0.50. Clinically healthy outdoor dogs have multiple CBC abnormalities, consistent with stress and low-level chronic inflammation, but not associated with a previous exposure to Anaplasma spp. or Babesia spp. The presence of microfilaria increases haemolysis in vitro., Klinički zdravi psi koji borave na otvorenom i koji su izloženi patogenima koje prenose vektori mogu imati hematološke premećaje vezane za dejstvo tih patogena. Ipak, hematološke pretrage se retko izvode na toj populaciji pasa. Stoga je cilj našeg rada bio da utvrdimo promene u krvnoj slici zdravih pasa lutalica, pasa smeštenih u azilima i lovačkih pasa, seroreaktivnih na uzročnike bolesti iz rodova Anaplasma i Babesia, sa ili bez mikrofilarioze. U ovom radu je ispitana krvna slika 81 psa. U studiju su uključeni psi kod kojih lančanom reakcijom polimerizacije nije pokazano prisustvo DNK Anaplasma spp. i Babesia spp. Psi su prema serološkom statusu raspoređeni u sledeće grupe: seronegativni na Anaplasma spp. i Babesia spp. SN (N=26), seroreaktivni na A. phagocytophilum SR-A (N=12), seroreaktivni na B. canis, B. gibsoni i/ili B. vogeli SR-B (N=25) i seroreaktivni na oba navedena patogena SR-AB (N=8). Ove četiri grupe su bile negativne na mikrofilariozu, dok je peta grupa, seroreaktivna na neki ili oba patogena imala i mikrofilarije SR-M (N=10). Učestalost promena kompletne krvne slike među svim ispitivanim psima je bila sledeća: 0,35 za leukocitozu, 0,44 za granulocitozu, 0,28 za anemiju, 0,74 za mikrocitozu, 0,37 za povećanu prosečnu koncentraciju hemoglobina u eritrocitima i 0,33 za trombocitopeniju. Grupe se nisu razlikovale u pogledu učestalosti navedenih promena. Izuzetak je grupa SR-M u kojoj je učestalost povećanja vrednosti za prosečnu koncentraciju hemoglobina u eritrocitima iznosila 0,80. U ostale četiri grupe ta učestalost je iznosila između 0,10 i 0,50. Zaključak je da ispitivani psi imaju višestruke poremećaje krvne slike koji najverovatnije odražavaju stres i nizak stepen hronične inflamacije, ali ne i vezu sa prethodnim kontaktom sa Anaplasma spp. i Babesia spp. Prisustvo mikrofilarija izaziva hemolizu in vitro.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Hаematologic indices in clinically healthy outdoor dogs exposed to vector-borne pathogens, Hematološki parametri kod klinički zdravih pasa koji borave na otvorenom i izloženi su patogenima koje prenose vektori",
volume = "74",
number = "2",
pages = "178-186",
doi = "10.2298/VETGL191216005J"
}

Janjić, F., Milanović, Z., Ilić Božović, A., Andrić, N., Spariosu, K., Ajtić, J., Beletić, A.,& Kovačević-Filipović, M.. (2020). Hаematologic indices in clinically healthy outdoor dogs exposed to vector-borne pathogens. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 74(2), 178-186.
https://doi.org/10.2298/VETGL191216005J

Janjić F, Milanović Z, Ilić Božović A, Andrić N, Spariosu K, Ajtić J, Beletić A, Kovačević-Filipović M. Hаematologic indices in clinically healthy outdoor dogs exposed to vector-borne pathogens. in Veterinarski Glasnik. 2020;74(2):178-186.
doi:10.2298/VETGL191216005J .

Janjić, Filip, Milanović, Zorana, Ilić Božović, Anja, Andrić, Nenad, Spariosu, Kristina, Ajtić, Jelena, Beletić, Anđelo, Kovačević-Filipović, Milica, "Hаematologic indices in clinically healthy outdoor dogs exposed to vector-borne pathogens" in Veterinarski Glasnik, 74, no. 2 (2020):178-186,
https://doi.org/10.2298/VETGL191216005J . .

TY  - JOUR
AU  - Milanović, Zorana
AU  - Vekić, Jelena
AU  - Radonjić, Vladimir
AU  - Božović-Ilić, Anja
AU  - Zeljković, Aleksandra
AU  - Janac, Jelena
AU  - Spasojevic-Kalimanovska, Vesna
AU  - Buch, Jesse
AU  - Chandrashekar, Ramaswamy
AU  - Bojic-Trbojević, Zanka
AU  - Hajduković, Ljiljana
AU  - Christopher, Mary M.
AU  - Kovačević-Filipović, Milica
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1778
AB  - Background Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results Dogs with B. canis infection had a marked APR (median SAA, 168.3 mu g/mL; range, 98.1-716.2 mu g/mL) compared with controls (3.2 mu g/mL, 2.0-4.2 mu g/mL) (P < .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P = .02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P = .02), and alpha-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P = .04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P = .02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho = .43; P = .03) and ApoA-1 (rho = .63, P < .001). Conclusions and Clinical Importance Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.
PB  - Wiley, Hoboken
T2  - Journal of Veterinary Internal Medicine
T1  - Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs
VL  - 33
IS  - 4
SP  - 1686
EP  - 1694
DO  - 10.1111/jvim.15537
ER  - 

@article{
author = "Milanović, Zorana and Vekić, Jelena and Radonjić, Vladimir and Božović-Ilić, Anja and Zeljković, Aleksandra and Janac, Jelena and Spasojevic-Kalimanovska, Vesna and Buch, Jesse and Chandrashekar, Ramaswamy and Bojic-Trbojević, Zanka and Hajduković, Ljiljana and Christopher, Mary M. and Kovačević-Filipović, Milica",
year = "2019",
abstract = "Background Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results Dogs with B. canis infection had a marked APR (median SAA, 168.3 mu g/mL; range, 98.1-716.2 mu g/mL) compared with controls (3.2 mu g/mL, 2.0-4.2 mu g/mL) (P < .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P = .02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P = .02), and alpha-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P = .04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P = .02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho = .43; P = .03) and ApoA-1 (rho = .63, P < .001). Conclusions and Clinical Importance Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.",
publisher = "Wiley, Hoboken",
journal = "Journal of Veterinary Internal Medicine",
title = "Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs",
volume = "33",
number = "4",
pages = "1686-1694",
doi = "10.1111/jvim.15537"
}

Milanović, Z., Vekić, J., Radonjić, V., Božović-Ilić, A., Zeljković, A., Janac, J., Spasojevic-Kalimanovska, V., Buch, J., Chandrashekar, R., Bojic-Trbojević, Z., Hajduković, L., Christopher, M. M.,& Kovačević-Filipović, M.. (2019). Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. in Journal of Veterinary Internal Medicine
Wiley, Hoboken., 33(4), 1686-1694.
https://doi.org/10.1111/jvim.15537

Milanović Z, Vekić J, Radonjić V, Božović-Ilić A, Zeljković A, Janac J, Spasojevic-Kalimanovska V, Buch J, Chandrashekar R, Bojic-Trbojević Z, Hajduković L, Christopher MM, Kovačević-Filipović M. Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. in Journal of Veterinary Internal Medicine. 2019;33(4):1686-1694.
doi:10.1111/jvim.15537 .

Milanović, Zorana, Vekić, Jelena, Radonjić, Vladimir, Božović-Ilić, Anja, Zeljković, Aleksandra, Janac, Jelena, Spasojevic-Kalimanovska, Vesna, Buch, Jesse, Chandrashekar, Ramaswamy, Bojic-Trbojević, Zanka, Hajduković, Ljiljana, Christopher, Mary M., Kovačević-Filipović, Milica, "Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs" in Journal of Veterinary Internal Medicine, 33, no. 4 (2019):1686-1694,
https://doi.org/10.1111/jvim.15537 . .

TY  - JOUR
AU  - Danilović-Luković, Jelena
AU  - Korac, Aleksandra
AU  - Milošević, Ivan
AU  - Lužajić, Tijana
AU  - Milanović, Zorana
AU  - Kovačević-Filipović, Milica
AU  - Radovanović, Anita
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1477
AB  - Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/ oocytes was significantly lower in the hypothyroid group. No significant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly reflecting that these proteins were not involved in the oogonia/ oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/ oocytes altered with the advanced process of autophagy.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats
VL  - 67
IS  - 1
SP  - 92
EP  - 106
DO  - 10.1515/acve-2017-0009
ER  - 

@article{
author = "Danilović-Luković, Jelena and Korac, Aleksandra and Milošević, Ivan and Lužajić, Tijana and Milanović, Zorana and Kovačević-Filipović, Milica and Radovanović, Anita",
year = "2017",
abstract = "Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/ oocytes was significantly lower in the hypothyroid group. No significant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly reflecting that these proteins were not involved in the oogonia/ oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/ oocytes altered with the advanced process of autophagy.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats",
volume = "67",
number = "1",
pages = "92-106",
doi = "10.1515/acve-2017-0009"
}

Danilović-Luković, J., Korac, A., Milošević, I., Lužajić, T., Milanović, Z., Kovačević-Filipović, M.,& Radovanović, A.. (2017). Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(1), 92-106.
https://doi.org/10.1515/acve-2017-0009

Danilović-Luković J, Korac A, Milošević I, Lužajić T, Milanović Z, Kovačević-Filipović M, Radovanović A. Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats. in Acta Veterinaria-Beograd. 2017;67(1):92-106.
doi:10.1515/acve-2017-0009 .

Danilović-Luković, Jelena, Korac, Aleksandra, Milošević, Ivan, Lužajić, Tijana, Milanović, Zorana, Kovačević-Filipović, Milica, Radovanović, Anita, "Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats" in Acta Veterinaria-Beograd, 67, no. 1 (2017):92-106,
https://doi.org/10.1515/acve-2017-0009 . .

TY  - CONF
AU  - Kovačević Filipović, Milica
AU  - Francuski Andrić, Jelena
AU  - Davitkov, Darko
AU  - Ilić Božović, Anja
AU  - Milanović, Zorana
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3821
PB  - Beograd : Fakultet veterinarske medicine
C3  - Devetnaesto regionalno savetovanje iz kliničke patologije i terapije životinja Clinica veterinaria 2017, Fruška gora, 15 - 17. jun 2017
T1  - Pojedine vektorski prenosive bolesti pasa i mačaka – preporuke za dijagnostiku; značaj pregleda krvnog razmaza
SP  - 45
EP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3821
ER  - 

@conference{
author = "Kovačević Filipović, Milica and Francuski Andrić, Jelena and Davitkov, Darko and Ilić Božović, Anja and Milanović, Zorana",
year = "2017",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Devetnaesto regionalno savetovanje iz kliničke patologije i terapije životinja Clinica veterinaria 2017, Fruška gora, 15 - 17. jun 2017",
title = "Pojedine vektorski prenosive bolesti pasa i mačaka – preporuke za dijagnostiku; značaj pregleda krvnog razmaza",
pages = "45-46",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3821"
}

Kovačević Filipović, M., Francuski Andrić, J., Davitkov, D., Ilić Božović, A.,& Milanović, Z.. (2017). Pojedine vektorski prenosive bolesti pasa i mačaka – preporuke za dijagnostiku; značaj pregleda krvnog razmaza. in Devetnaesto regionalno savetovanje iz kliničke patologije i terapije životinja Clinica veterinaria 2017, Fruška gora, 15 - 17. jun 2017
Beograd : Fakultet veterinarske medicine., 45-46.
https://hdl.handle.net/21.15107/rcub_veterinar_3821

Kovačević Filipović M, Francuski Andrić J, Davitkov D, Ilić Božović A, Milanović Z. Pojedine vektorski prenosive bolesti pasa i mačaka – preporuke za dijagnostiku; značaj pregleda krvnog razmaza. in Devetnaesto regionalno savetovanje iz kliničke patologije i terapije životinja Clinica veterinaria 2017, Fruška gora, 15 - 17. jun 2017. 2017;:45-46.
https://hdl.handle.net/21.15107/rcub_veterinar_3821 .

Kovačević Filipović, Milica, Francuski Andrić, Jelena, Davitkov, Darko, Ilić Božović, Anja, Milanović, Zorana, "Pojedine vektorski prenosive bolesti pasa i mačaka – preporuke za dijagnostiku; značaj pregleda krvnog razmaza" in Devetnaesto regionalno savetovanje iz kliničke patologije i terapije životinja Clinica veterinaria 2017, Fruška gora, 15 - 17. jun 2017 (2017):45-46,
https://hdl.handle.net/21.15107/rcub_veterinar_3821 .

TY  - JOUR
AU  - Milanović, Zorana
AU  - Božović-Ilić, Anja
AU  - Francuski Andrić, Jelena
AU  - Radonjić, Vladimir
AU  - Beletić, Andelo
AU  - Kovačević-Filipović, Milica
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1520
AB  - Babesia canis and Dirofilaria immitis are emerging and geographically overlapping vector-borne pathogens in dogs. Infection with B. canis leads to acute-phase response (APR) that can be mild to severe and results in either non-complicated or complicated forms of the disease. The aim of this study was to determine whether acute B. canis infection is more severe in dogs with underlying asymptomatic D. immitis infection. Dogs of both sexes, different ages and breeds, with naturally occurring mono-infections with B. canis (n = 13) and D. immitis (n = 18) and co-infected dogs (n = 7) were enrolled as well as healthy controls (n = 15). Routine haematology and biochemistry, agarose gel electrophoresis (agEF) protein fraction separation and enzyme-linked immtmosorbent assay (ELISA) for serum amyloid A (SAA) were performed. Based on clinical and laboratory findings, sepsis was diagnosed in the majority of dogs with acute B. cants infection with or without underlying asymptomatic D. immitis infection. Overall, haematology, biochemistry and agEF pattern changes were induced and dictated by acute B. cards infection whether or not the dogs had an asymptomatic D. immitis infection. D. immitis infection slightly influenced the level of anaemia, slightly aggravated the level of dehydration and increased the concentration of gamma-globulins in acute-phase B. canis infection. D. immitis infection prevented B. canis-induced leukopenia. SAA equally increased in dogs with acute B. cans infection with or without underlying D. immitis infection. The level of SAA was not changed in dogs with asymptomatic D. immitis when compared to the controls. In conclusion, asymptomatic D. immitis infection does not influence overall APR after acute B. canis infection.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Ticks and Tick-Borne Diseases
T1  - Acute-phase response in Babesia canis and Dirofilaria immitis co-infections in dogs
VL  - 8
IS  - 6
SP  - 907
EP  - 914
DO  - 10.1016/j.ttbdis.2017.07.009
ER  - 

@article{
author = "Milanović, Zorana and Božović-Ilić, Anja and Francuski Andrić, Jelena and Radonjić, Vladimir and Beletić, Andelo and Kovačević-Filipović, Milica",
year = "2017",
abstract = "Babesia canis and Dirofilaria immitis are emerging and geographically overlapping vector-borne pathogens in dogs. Infection with B. canis leads to acute-phase response (APR) that can be mild to severe and results in either non-complicated or complicated forms of the disease. The aim of this study was to determine whether acute B. canis infection is more severe in dogs with underlying asymptomatic D. immitis infection. Dogs of both sexes, different ages and breeds, with naturally occurring mono-infections with B. canis (n = 13) and D. immitis (n = 18) and co-infected dogs (n = 7) were enrolled as well as healthy controls (n = 15). Routine haematology and biochemistry, agarose gel electrophoresis (agEF) protein fraction separation and enzyme-linked immtmosorbent assay (ELISA) for serum amyloid A (SAA) were performed. Based on clinical and laboratory findings, sepsis was diagnosed in the majority of dogs with acute B. cants infection with or without underlying asymptomatic D. immitis infection. Overall, haematology, biochemistry and agEF pattern changes were induced and dictated by acute B. cards infection whether or not the dogs had an asymptomatic D. immitis infection. D. immitis infection slightly influenced the level of anaemia, slightly aggravated the level of dehydration and increased the concentration of gamma-globulins in acute-phase B. canis infection. D. immitis infection prevented B. canis-induced leukopenia. SAA equally increased in dogs with acute B. cans infection with or without underlying D. immitis infection. The level of SAA was not changed in dogs with asymptomatic D. immitis when compared to the controls. In conclusion, asymptomatic D. immitis infection does not influence overall APR after acute B. canis infection.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Ticks and Tick-Borne Diseases",
title = "Acute-phase response in Babesia canis and Dirofilaria immitis co-infections in dogs",
volume = "8",
number = "6",
pages = "907-914",
doi = "10.1016/j.ttbdis.2017.07.009"
}

Milanović, Z., Božović-Ilić, A., Francuski Andrić, J., Radonjić, V., Beletić, A.,& Kovačević-Filipović, M.. (2017). Acute-phase response in Babesia canis and Dirofilaria immitis co-infections in dogs. in Ticks and Tick-Borne Diseases
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 8(6), 907-914.
https://doi.org/10.1016/j.ttbdis.2017.07.009

Milanović Z, Božović-Ilić A, Francuski Andrić J, Radonjić V, Beletić A, Kovačević-Filipović M. Acute-phase response in Babesia canis and Dirofilaria immitis co-infections in dogs. in Ticks and Tick-Borne Diseases. 2017;8(6):907-914.
doi:10.1016/j.ttbdis.2017.07.009 .

Milanović, Zorana, Božović-Ilić, Anja, Francuski Andrić, Jelena, Radonjić, Vladimir, Beletić, Andelo, Kovačević-Filipović, Milica, "Acute-phase response in Babesia canis and Dirofilaria immitis co-infections in dogs" in Ticks and Tick-Borne Diseases, 8, no. 6 (2017):907-914,
https://doi.org/10.1016/j.ttbdis.2017.07.009 . .

TY  - JOUR
AU  - Kovacić, Marijana
AU  - Marković, Dragana
AU  - Maslovarić, Irina
AU  - Obrenović, Sonja
AU  - Grujic-Milanović, Jelica
AU  - Arsić, Aleksandra
AU  - Milanović, Zorana
AU  - Savić, Olivera
AU  - Fratrić, Natalija
AU  - Ilić, Vesna
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1478
AB  - Calf bronchopneumonia is complex multifactorial disease and for its accurate diagnosis and therapy, besides clinical examination, microbiologic, hematologic and biochemical analyses could be necessary. In general, additional analyses are not implemented, mainly because the disease biomarkers are not defined. To establish which analysis might be useful for determining the severity of the disease, we analyzed 23 three-month old calves with mild clinical signs of bronchopneumonia and 15 age-matched healthy calves. Pasteurella multocida was isolated from deep nasal swabs of diseased calves. Peripheral blood erythrocyte and leukocyte count of bronchopneumonic and healthy calves showed no difference. Serum proteins, lipoproteins and lipids were analyzed with spectrophotometry, agarose gel electrophoresis, non-reducing SDSPAGE, gel zymography, and thin-layer chromatography. The bronchopneumonic calves had an increased level of circulating immune complexes and a globulins, which contain some of the positive acute phase proteins. In diseased calves the increased concentration of total. globulins (IgG), due to an increased concentration of anionic. globulins (predominately IgG1), was detected. The increased concentration of anionic. globulins followed by increased concentration of transferrin (negative acute phase protein) and HDL cholesterol, decreased concentration of LDL-cholesterol, unchanged activity of matrix metalloproteases and leukocyte counts might reflect the obvious absence of generalized inflammation. A positive correlation was found between the acquired results and the appearance of mild clinical signs. Therefore, we believe that the parameters analyzed in the peripheral blood could be applied as reliable disease markers to distinguish between severe (inflammatory) and mild forms of calf bronchopneumonia and to predict a better outcome for these calves.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers
VL  - 67
IS  - 2
SP  - 207
EP  - 227
DO  - 10.1515/acve-2017-0018
ER  - 

@article{
author = "Kovacić, Marijana and Marković, Dragana and Maslovarić, Irina and Obrenović, Sonja and Grujic-Milanović, Jelica and Arsić, Aleksandra and Milanović, Zorana and Savić, Olivera and Fratrić, Natalija and Ilić, Vesna",
year = "2017",
abstract = "Calf bronchopneumonia is complex multifactorial disease and for its accurate diagnosis and therapy, besides clinical examination, microbiologic, hematologic and biochemical analyses could be necessary. In general, additional analyses are not implemented, mainly because the disease biomarkers are not defined. To establish which analysis might be useful for determining the severity of the disease, we analyzed 23 three-month old calves with mild clinical signs of bronchopneumonia and 15 age-matched healthy calves. Pasteurella multocida was isolated from deep nasal swabs of diseased calves. Peripheral blood erythrocyte and leukocyte count of bronchopneumonic and healthy calves showed no difference. Serum proteins, lipoproteins and lipids were analyzed with spectrophotometry, agarose gel electrophoresis, non-reducing SDSPAGE, gel zymography, and thin-layer chromatography. The bronchopneumonic calves had an increased level of circulating immune complexes and a globulins, which contain some of the positive acute phase proteins. In diseased calves the increased concentration of total. globulins (IgG), due to an increased concentration of anionic. globulins (predominately IgG1), was detected. The increased concentration of anionic. globulins followed by increased concentration of transferrin (negative acute phase protein) and HDL cholesterol, decreased concentration of LDL-cholesterol, unchanged activity of matrix metalloproteases and leukocyte counts might reflect the obvious absence of generalized inflammation. A positive correlation was found between the acquired results and the appearance of mild clinical signs. Therefore, we believe that the parameters analyzed in the peripheral blood could be applied as reliable disease markers to distinguish between severe (inflammatory) and mild forms of calf bronchopneumonia and to predict a better outcome for these calves.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers",
volume = "67",
number = "2",
pages = "207-227",
doi = "10.1515/acve-2017-0018"
}

Kovacić, M., Marković, D., Maslovarić, I., Obrenović, S., Grujic-Milanović, J., Arsić, A., Milanović, Z., Savić, O., Fratrić, N.,& Ilić, V.. (2017). Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(2), 207-227.
https://doi.org/10.1515/acve-2017-0018

Kovacić M, Marković D, Maslovarić I, Obrenović S, Grujic-Milanović J, Arsić A, Milanović Z, Savić O, Fratrić N, Ilić V. Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers. in Acta Veterinaria-Beograd. 2017;67(2):207-227.
doi:10.1515/acve-2017-0018 .

Kovacić, Marijana, Marković, Dragana, Maslovarić, Irina, Obrenović, Sonja, Grujic-Milanović, Jelica, Arsić, Aleksandra, Milanović, Zorana, Savić, Olivera, Fratrić, Natalija, Ilić, Vesna, "Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers" in Acta Veterinaria-Beograd, 67, no. 2 (2017):207-227,
https://doi.org/10.1515/acve-2017-0018 . .

TY  - CONF
AU  - Kovačević Filipović, Milica
AU  - Ilić, Anja
AU  - Milanović, Zorana
AU  - Bogdanović, Dragan
AU  - Radonjić, Vladimir
AU  - Francuski Andrić, Jelena
AU  - Andrić, Nenad
AU  - Thatcher, Brendon
AU  - Leutenegger, Christian
AU  - Tyrrell, Phyllis
AU  - Mestek, Antony
AU  - Ramaswamy, Chandrashekar
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3644
PB  - Journal of Veterinary Internal Medicine
C3  - 2016 ACVIM Forum Research Abstract Program Denver, Colorado, June 9 – 10, 2016
T1  - Molecular analysis of canine babesia prevalence and exposure to anaplasma, ehrlichia, borrelia and dirofilaria in most populated Serbian region – Belgrade area
SP  - 1474
EP  - 1474
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3644
ER  - 

@conference{
author = "Kovačević Filipović, Milica and Ilić, Anja and Milanović, Zorana and Bogdanović, Dragan and Radonjić, Vladimir and Francuski Andrić, Jelena and Andrić, Nenad and Thatcher, Brendon and Leutenegger, Christian and Tyrrell, Phyllis and Mestek, Antony and Ramaswamy, Chandrashekar",
year = "2016",
publisher = "Journal of Veterinary Internal Medicine",
journal = "2016 ACVIM Forum Research Abstract Program Denver, Colorado, June 9 – 10, 2016",
title = "Molecular analysis of canine babesia prevalence and exposure to anaplasma, ehrlichia, borrelia and dirofilaria in most populated Serbian region – Belgrade area",
pages = "1474-1474",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3644"
}

Kovačević Filipović, M., Ilić, A., Milanović, Z., Bogdanović, D., Radonjić, V., Francuski Andrić, J., Andrić, N., Thatcher, B., Leutenegger, C., Tyrrell, P., Mestek, A.,& Ramaswamy, C.. (2016). Molecular analysis of canine babesia prevalence and exposure to anaplasma, ehrlichia, borrelia and dirofilaria in most populated Serbian region – Belgrade area. in 2016 ACVIM Forum Research Abstract Program Denver, Colorado, June 9 – 10, 2016
Journal of Veterinary Internal Medicine., 1474-1474.
https://hdl.handle.net/21.15107/rcub_veterinar_3644

Kovačević Filipović M, Ilić A, Milanović Z, Bogdanović D, Radonjić V, Francuski Andrić J, Andrić N, Thatcher B, Leutenegger C, Tyrrell P, Mestek A, Ramaswamy C. Molecular analysis of canine babesia prevalence and exposure to anaplasma, ehrlichia, borrelia and dirofilaria in most populated Serbian region – Belgrade area. in 2016 ACVIM Forum Research Abstract Program Denver, Colorado, June 9 – 10, 2016. 2016;:1474-1474.
https://hdl.handle.net/21.15107/rcub_veterinar_3644 .

Kovačević Filipović, Milica, Ilić, Anja, Milanović, Zorana, Bogdanović, Dragan, Radonjić, Vladimir, Francuski Andrić, Jelena, Andrić, Nenad, Thatcher, Brendon, Leutenegger, Christian, Tyrrell, Phyllis, Mestek, Antony, Ramaswamy, Chandrashekar, "Molecular analysis of canine babesia prevalence and exposure to anaplasma, ehrlichia, borrelia and dirofilaria in most populated Serbian region – Belgrade area" in 2016 ACVIM Forum Research Abstract Program Denver, Colorado, June 9 – 10, 2016 (2016):1474-1474,
https://hdl.handle.net/21.15107/rcub_veterinar_3644 .