TY  - CONF
AU  - Jovanović, Nemanja M.
AU  - Bisenić, Olga
AU  - Nenadović, Katarina
AU  - Bogunović, Danica
AU  - Rajković, Milan
AU  - Maletić, Milan
AU  - Ilić, Tamara
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3895
AB  - Пси су најпопуларнији кућни љубимци широм света. Близак контакт паса и људи повећава ризик преношења различитих зоонозних паразитских инфекција. С обзиром на значај ветеринарске медицине у очувању концепта Једног здравља, ово истраживање је имало за циљ да се идентификују интестинални паразити који могу имати зоонозни потенцијал и утичу на здравље паса и изврши процена фактора ризика (индивидуални и фактори средине) који условљавају појаву интестиналних паразитских инфекција. Истраживање је спроведено на територији Републике Србије на 382 власничка пса у току 2022. и 2023. године, квалитативним методама копролошке дијагностике са концентрацијом паразитских елемената. Укупна преваленција интестиналних паразита је била 58,7%, а утврђене су протозое: Cystoisospora spp. (9,2%), Sarcocystis spp. (4,5%), Neospora caninum/Hammondia spp. (3,7%), Giardia intestinalis (11,8%); нематоде: Toxocara canis (11,5%), Toxascaris leonina (4,2%), фам. Ancylostomatidae (38,0%), Trichuris vulpis (21,5%), Capillaria spp. (10,5%), трематода Alaria alata (1,6%) и цестоде фамилије Taeniidae (1,3%). Анализом индивидуалних фактора ризика, значајно виша (p<0,05) преваленција интестиналних паразита је утвђена код паса млађих од једне године (83,3%) и код паса старости од једне до пет година (64,4%). Анализом фактора ризика средине, значајно више (p<0,001) преваленцијe су утврђене у категоријама паса који се држе напољу (67,7%) и код паса који се држе у затвореном простору и напољу (72,0%), као и код категорије ловачких паса (81,6%). Додатно, значајно виша (p<0,05) преваленција интестиналних паразита је установљена и у категорији паса који се хране мешовито (72,5%). Резултати овог истраживања сугеришу да је неопходна едукација власника, спровођење редовних паразитолошких прегледа власничких паса и дехелминтизација у складу са налазима добијене копролошке дијагностике.
AB  - Dogs are the most popular pets worldwide. Close contact between dogs and people increases the risk of transmission of different zoonotic parasitic infections. Given the importance of veterinary medicine in preserving the One Health concept, the aim of this research was to identify the intestinal parasites that may have zoonotic potential and affect the health of dogs and to evaluate the risk factors (individual and environmental factors) that determine the occurrence of intestinal parasitic infections. The research was conducted on the territory of the Republic of Serbia in 2022 and 2023 on 382 owned dogs, using qualitative methods of coprological examination with concentration of parasitic elements. The overall prevalence of intestinal parasites was 58.7%, with detected protozoa: Cystoisospora spp. (9.2%), Sarcocystis spp. (4.5%), Neospora caninum/Hammondia spp. (3.7%), Giardia intestinalis (11.8%); nematoda: Toxocara canis (11.5%), Toxascaris leonina (4.2%), fam. Ancylostomatidae (38.0%), Trichuris vulpis (21,5%), Capillaria spp. (10.5%); trematoda: Alaria alata (1.6%) and cestodes from the Taeniidae family (1.3%). By analyzing the individual risk factors, a significantly higher (p<0.05) prevalence of intestinal parasites was found in dogs younger than one year (83.3%) and in dogs between one and five years old (64.4%). By analyzing the environmental risk factors, a significantly higher (p<0.001) prevalence was found in the dogs that were kept outdoor (67.7%) and indoor/outdoor (72.0%), as well as in the category of hunting dogs (81.6%). Additionally, a significantly higher (p<0.05) prevalence of intestinal parasites was found in the category of dogs fed combined diet (72.5%). Based on the results of coprological diagnostics, this research indicates the importance of educating dog owners, conducting routine parasitological tests on their pets and regular deworming strategies.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024
T1  - Intestinalne parazitske infekcije vlasničkih pasa i njihov značaj za jedno zdravlje u Republici Srbiji
T1  - Intestinal parasitic infections of owned dogs and their importance for one health in the Republic of Serbia
SP  - 46
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3895
ER  - 

@conference{
author = "Jovanović, Nemanja M. and Bisenić, Olga and Nenadović, Katarina and Bogunović, Danica and Rajković, Milan and Maletić, Milan and Ilić, Tamara",
year = "2024",
abstract = "Пси су најпопуларнији кућни љубимци широм света. Близак контакт паса и људи повећава ризик преношења различитих зоонозних паразитских инфекција. С обзиром на значај ветеринарске медицине у очувању концепта Једног здравља, ово истраживање је имало за циљ да се идентификују интестинални паразити који могу имати зоонозни потенцијал и утичу на здравље паса и изврши процена фактора ризика (индивидуални и фактори средине) који условљавају појаву интестиналних паразитских инфекција. Истраживање је спроведено на територији Републике Србије на 382 власничка пса у току 2022. и 2023. године, квалитативним методама копролошке дијагностике са концентрацијом паразитских елемената. Укупна преваленција интестиналних паразита је била 58,7%, а утврђене су протозое: Cystoisospora spp. (9,2%), Sarcocystis spp. (4,5%), Neospora caninum/Hammondia spp. (3,7%), Giardia intestinalis (11,8%); нематоде: Toxocara canis (11,5%), Toxascaris leonina (4,2%), фам. Ancylostomatidae (38,0%), Trichuris vulpis (21,5%), Capillaria spp. (10,5%), трематода Alaria alata (1,6%) и цестоде фамилије Taeniidae (1,3%). Анализом индивидуалних фактора ризика, значајно виша (p<0,05) преваленција интестиналних паразита је утвђена код паса млађих од једне године (83,3%) и код паса старости од једне до пет година (64,4%). Анализом фактора ризика средине, значајно више (p<0,001) преваленцијe су утврђене у категоријама паса који се држе напољу (67,7%) и код паса који се држе у затвореном простору и напољу (72,0%), као и код категорије ловачких паса (81,6%). Додатно, значајно виша (p<0,05) преваленција интестиналних паразита је установљена и у категорији паса који се хране мешовито (72,5%). Резултати овог истраживања сугеришу да је неопходна едукација власника, спровођење редовних паразитолошких прегледа власничких паса и дехелминтизација у складу са налазима добијене копролошке дијагностике., Dogs are the most popular pets worldwide. Close contact between dogs and people increases the risk of transmission of different zoonotic parasitic infections. Given the importance of veterinary medicine in preserving the One Health concept, the aim of this research was to identify the intestinal parasites that may have zoonotic potential and affect the health of dogs and to evaluate the risk factors (individual and environmental factors) that determine the occurrence of intestinal parasitic infections. The research was conducted on the territory of the Republic of Serbia in 2022 and 2023 on 382 owned dogs, using qualitative methods of coprological examination with concentration of parasitic elements. The overall prevalence of intestinal parasites was 58.7%, with detected protozoa: Cystoisospora spp. (9.2%), Sarcocystis spp. (4.5%), Neospora caninum/Hammondia spp. (3.7%), Giardia intestinalis (11.8%); nematoda: Toxocara canis (11.5%), Toxascaris leonina (4.2%), fam. Ancylostomatidae (38.0%), Trichuris vulpis (21,5%), Capillaria spp. (10.5%); trematoda: Alaria alata (1.6%) and cestodes from the Taeniidae family (1.3%). By analyzing the individual risk factors, a significantly higher (p<0.05) prevalence of intestinal parasites was found in dogs younger than one year (83.3%) and in dogs between one and five years old (64.4%). By analyzing the environmental risk factors, a significantly higher (p<0.001) prevalence was found in the dogs that were kept outdoor (67.7%) and indoor/outdoor (72.0%), as well as in the category of hunting dogs (81.6%). Additionally, a significantly higher (p<0.05) prevalence of intestinal parasites was found in the category of dogs fed combined diet (72.5%). Based on the results of coprological diagnostics, this research indicates the importance of educating dog owners, conducting routine parasitological tests on their pets and regular deworming strategies.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024",
title = "Intestinalne parazitske infekcije vlasničkih pasa i njihov značaj za jedno zdravlje u Republici Srbiji, Intestinal parasitic infections of owned dogs and their importance for one health in the Republic of Serbia",
pages = "46-47",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3895"
}

Jovanović, N. M., Bisenić, O., Nenadović, K., Bogunović, D., Rajković, M., Maletić, M.,& Ilić, T.. (2024). Intestinalne parazitske infekcije vlasničkih pasa i njihov značaj za jedno zdravlje u Republici Srbiji. in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 46-47.
https://hdl.handle.net/21.15107/rcub_veterinar_3895

Jovanović NM, Bisenić O, Nenadović K, Bogunović D, Rajković M, Maletić M, Ilić T. Intestinalne parazitske infekcije vlasničkih pasa i njihov značaj za jedno zdravlje u Republici Srbiji. in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024. 2024;:46-47.
https://hdl.handle.net/21.15107/rcub_veterinar_3895 .

Jovanović, Nemanja M., Bisenić, Olga, Nenadović, Katarina, Bogunović, Danica, Rajković, Milan, Maletić, Milan, Ilić, Tamara, "Intestinalne parazitske infekcije vlasničkih pasa i njihov značaj za jedno zdravlje u Republici Srbiji" in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024 (2024):46-47,
https://hdl.handle.net/21.15107/rcub_veterinar_3895 .

TY  - CONF
AU  - Ilić, Tamara
AU  - Aleksić, Nevenka
AU  - Bogunović, Danica
AU  - Rajković, Milan
AU  - Stepanović, Predrag
AU  - Jovanović, Nemanja M.
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3713
AB  - U okviru parazitologije poslednjih decenija u Srbiji gotovo isključivo su se izučavale infekcije prouzrokovane endoparazitima krvi, digestivnog i respiratornog sistema, kao i artropode – ektoparaziti kože
ili pak vektori oboljenja različite etiologije. U skladu sa time, favorizovane su koprološke, hematološke i dermatološke metode dijagnostike. Urološkim metodama nije se pridavao poseban značaj, jer se polazilo od pretpostavke da na ovom području ne postoje preduslovi za
infekciju domaćih životinja parazitima urinarnog sistema. Globalno zagrevanje, povećanje prosečne temperature i obilne padavine, kao i
povećana mobilnost ljudi i životinja uslovili su stvaranje povoljnih uslova za pojavu, održavanje i širenje infekcija koje ranije nisu bile prisutne. Izvesna parazitološka ispitivanja divljih životinja rezultirala su prvim nalazom nematode Capillaria plica 2019. godine kod crvenih lisica
sa područja Kolubarskog okruga. Kasnije je utvrđeno prisustvo i potvrđena dijagnoza urinarne kapilarioze kod lovačkih pasa u različitim
delovima Srbije. Ovaj nalaz podstakao je produžetak istraživanja mogućeg prisustva drugih nematoda urinarnog sistema divljih i domaćih
mesojeda. Jedna od njih je i vrsta Dioctophyma renale, koja poseduje visok zoonozni potencijal. Zbog rizika koje D. renale može imati po
zdravlje vlasnika inficiranih pasa i mogućnosti da njeno prisustvo ne
bude zapaženo, potrebno je doktorima veterinarske medicine u maloj praksi i na terenu sugerisati da u diferencijalnoj dijagnostici parazitskih infekcija i oboljenja urinarnog trakta mesojeda obrate pažnju i
na ove helmintoze.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024
T1  - Urinarne parazitoze mesojeda – dijagnostički pristup i značaj za veterinarsku praksu
SP  - 55
EP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3713
ER  - 

@conference{
author = "Ilić, Tamara and Aleksić, Nevenka and Bogunović, Danica and Rajković, Milan and Stepanović, Predrag and Jovanović, Nemanja M.",
year = "2024",
abstract = "U okviru parazitologije poslednjih decenija u Srbiji gotovo isključivo su se izučavale infekcije prouzrokovane endoparazitima krvi, digestivnog i respiratornog sistema, kao i artropode – ektoparaziti kože
ili pak vektori oboljenja različite etiologije. U skladu sa time, favorizovane su koprološke, hematološke i dermatološke metode dijagnostike. Urološkim metodama nije se pridavao poseban značaj, jer se polazilo od pretpostavke da na ovom području ne postoje preduslovi za
infekciju domaćih životinja parazitima urinarnog sistema. Globalno zagrevanje, povećanje prosečne temperature i obilne padavine, kao i
povećana mobilnost ljudi i životinja uslovili su stvaranje povoljnih uslova za pojavu, održavanje i širenje infekcija koje ranije nisu bile prisutne. Izvesna parazitološka ispitivanja divljih životinja rezultirala su prvim nalazom nematode Capillaria plica 2019. godine kod crvenih lisica
sa područja Kolubarskog okruga. Kasnije je utvrđeno prisustvo i potvrđena dijagnoza urinarne kapilarioze kod lovačkih pasa u različitim
delovima Srbije. Ovaj nalaz podstakao je produžetak istraživanja mogućeg prisustva drugih nematoda urinarnog sistema divljih i domaćih
mesojeda. Jedna od njih je i vrsta Dioctophyma renale, koja poseduje visok zoonozni potencijal. Zbog rizika koje D. renale može imati po
zdravlje vlasnika inficiranih pasa i mogućnosti da njeno prisustvo ne
bude zapaženo, potrebno je doktorima veterinarske medicine u maloj praksi i na terenu sugerisati da u diferencijalnoj dijagnostici parazitskih infekcija i oboljenja urinarnog trakta mesojeda obrate pažnju i
na ove helmintoze.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024",
title = "Urinarne parazitoze mesojeda – dijagnostički pristup i značaj za veterinarsku praksu",
pages = "55-67",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3713"
}

Ilić, T., Aleksić, N., Bogunović, D., Rajković, M., Stepanović, P.,& Jovanović, N. M.. (2024). Urinarne parazitoze mesojeda – dijagnostički pristup i značaj za veterinarsku praksu. in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 55-67.
https://hdl.handle.net/21.15107/rcub_veterinar_3713

Ilić T, Aleksić N, Bogunović D, Rajković M, Stepanović P, Jovanović NM. Urinarne parazitoze mesojeda – dijagnostički pristup i značaj za veterinarsku praksu. in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024. 2024;:55-67.
https://hdl.handle.net/21.15107/rcub_veterinar_3713 .

Ilić, Tamara, Aleksić, Nevenka, Bogunović, Danica, Rajković, Milan, Stepanović, Predrag, Jovanović, Nemanja M., "Urinarne parazitoze mesojeda – dijagnostički pristup i značaj za veterinarsku praksu" in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024 (2024):55-67,
https://hdl.handle.net/21.15107/rcub_veterinar_3713 .

TY  - JOUR
AU  - Jovanović, Nemanja M.
AU  - Bisenić, Olga
AU  - Nenadović, Katarina
AU  - Bogunović, Danica
AU  - Rajković, Milan
AU  - Maletić, Milan
AU  - Mirilović, Milorad
AU  - Ilić, Tamara
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3882
AB  - Dogs are the most popular pets worldwide. Close contact between dogs and people
increases the risk of transmission of various zoonotic parasitic infections. Given the importance of
veterinary medicine in preserving the One Health concept, the aim of this research was to identify
intestinal parasites that may have zoonotic potential and to evaluate risk factors (individual and
environmental). The research was conducted in Serbia in 2022 and 2023 on 382 owned dogs, using
qualitative methods of coprological examination with a concentration on parasitic elements. The overall
prevalence of intestinal parasites was 62.6%, with the following detected: protozoa: Cystoisospora
spp. (9.2%), Sarcocystis spp. (4.5%), Neospora caninum/Hammondia spp. (3.7%), Giardia intestinalis
(11.8%); nematoda: Toxocara canis (11.5%), Toxascaris leonina (4.2%), family Ancylostomatidae (38.0%),
Trichuris vulpis (21.5%), Capillaria spp. (10.5%); trematoda: Alaria alata (1.6%) and cestodes from the
Taeniidae family (1.3%). Factors like age, size and coat length, as well as the way of living, attitude
and diet were linked to a significantly higher (p < 0.05) prevalence of intestinal parasites. Based on the
results of coprological diagnostics, this research indicates the importance of educating dog owners,
conducting routine parasitological tests on their pets and regular deworming strategies.
PB  - MDPI
T2  - Animals
T1  - Gastrointestinal parasites in owned dogs in Serbia: Prevalence and risk factors
VL  - 14
IS  - 10
SP  - 1463
DO  - 10.3390/ani14101463
ER  - 

@article{
author = "Jovanović, Nemanja M. and Bisenić, Olga and Nenadović, Katarina and Bogunović, Danica and Rajković, Milan and Maletić, Milan and Mirilović, Milorad and Ilić, Tamara",
year = "2024",
abstract = "Dogs are the most popular pets worldwide. Close contact between dogs and people
increases the risk of transmission of various zoonotic parasitic infections. Given the importance of
veterinary medicine in preserving the One Health concept, the aim of this research was to identify
intestinal parasites that may have zoonotic potential and to evaluate risk factors (individual and
environmental). The research was conducted in Serbia in 2022 and 2023 on 382 owned dogs, using
qualitative methods of coprological examination with a concentration on parasitic elements. The overall
prevalence of intestinal parasites was 62.6%, with the following detected: protozoa: Cystoisospora
spp. (9.2%), Sarcocystis spp. (4.5%), Neospora caninum/Hammondia spp. (3.7%), Giardia intestinalis
(11.8%); nematoda: Toxocara canis (11.5%), Toxascaris leonina (4.2%), family Ancylostomatidae (38.0%),
Trichuris vulpis (21.5%), Capillaria spp. (10.5%); trematoda: Alaria alata (1.6%) and cestodes from the
Taeniidae family (1.3%). Factors like age, size and coat length, as well as the way of living, attitude
and diet were linked to a significantly higher (p < 0.05) prevalence of intestinal parasites. Based on the
results of coprological diagnostics, this research indicates the importance of educating dog owners,
conducting routine parasitological tests on their pets and regular deworming strategies.",
publisher = "MDPI",
journal = "Animals",
title = "Gastrointestinal parasites in owned dogs in Serbia: Prevalence and risk factors",
volume = "14",
number = "10",
pages = "1463",
doi = "10.3390/ani14101463"
}

Jovanović, N. M., Bisenić, O., Nenadović, K., Bogunović, D., Rajković, M., Maletić, M., Mirilović, M.,& Ilić, T.. (2024). Gastrointestinal parasites in owned dogs in Serbia: Prevalence and risk factors. in Animals
MDPI., 14(10), 1463.
https://doi.org/10.3390/ani14101463

Jovanović NM, Bisenić O, Nenadović K, Bogunović D, Rajković M, Maletić M, Mirilović M, Ilić T. Gastrointestinal parasites in owned dogs in Serbia: Prevalence and risk factors. in Animals. 2024;14(10):1463.
doi:10.3390/ani14101463 .

Jovanović, Nemanja M., Bisenić, Olga, Nenadović, Katarina, Bogunović, Danica, Rajković, Milan, Maletić, Milan, Mirilović, Milorad, Ilić, Tamara, "Gastrointestinal parasites in owned dogs in Serbia: Prevalence and risk factors" in Animals, 14, no. 10 (2024):1463,
https://doi.org/10.3390/ani14101463 . .

TY  - CONF
AU  - Rajković, Milan
AU  - Glavinić, Uroš
AU  - Bogunović, Danica
AU  - Jovanović, Nemanja M.
AU  - Ristanić, Marko
AU  - Blagojević, Jovan
AU  - Stanimirović, Zoran
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3865
AB  - Dirofilaria immitis је један од најчешћих паразита који погађа домаће и дивље
месоједе, а преносе га комарци из родова Culex, Aedes и Anopheles. Поред адулта
који су локализовани у плућним артеријама, циркулишуће микрофиларије,
такође, имају улогу у покретању инфламаторног одговора домаћина који је
кључан за патогенезу и даљи развој болести. Реактивне врсте кисеоника (ROS)
које настају током инфламаторног одговора су важан део одбрамбених
стратегија организама за елиминацију паразита, али могу довести и до
оксидативног оштећења различитих молекула укључујући и ДНК. Ово може
резултирати појавом мутација, грешака у репликацији и нестабилности читавог
генома домаћина. Стога је циљ овог истраживања била процена степена
оштећења ДНК у леукоцитима паса инфицираних врстом D. immitis, пре, током
и након примене антипаразитске терапије, употребом in vivo комет теста.
Додатно, праћена је њихова осетљивост на присуство мутагена (H2O2) у ex vivo
комет тесту. Коришћени су узорци пуне крви од 34 пса пореклом из Србије,
старости од једне до 13 година, оба пола. Процена генотоксичности је показала
да инфекција узрокована врстом D. immitis доводи до оштећења ДНК код
природно заражених паса, при чему се највећа оштећења ДНК јављају у групи
паса са тежим клиничким знацима. Третман ивермектином и доксициклином
смањио је оштећење ДНК у леукоцитима паса у свим групама и то услед
смањења интензитета инфекције и оксидативног стреса. Резултати ex vivo комет
теста показали су повећану осетљивост леукоцита на оштећење ДНК изазвано
водоокник-пероксидом (H2O2) пре терапије, док је примењена терапија довела
до повећања резистенције леукоцита на оштећење ДНК. Добијени резултати
указују да је благовремено постављање дијагнозе и отпочињање терапије од
великог значаја за смањење доказаних негативих ефеката инфекције врстом D.
immitis, укључујући и оштећење ДНК домаћина.
AB  - Dirofilaria immitis is one of the most common parasites affecting domestic and wild
carnivores, and it is transmitted by mosquitoes from the genera Culex, Aedes and
Anopheles. In addition to adults localized in the pulmonary arteries, circulating
microfilariae also plays a significant role in triggering the host's inflammatory
response, which is crucial for the pathogenesis and further development of the disease.
Reactive oxygen species (ROS) generated during the inflammatory response are an
important part of the defense of the organism in the elimination of the parasites but
can also lead to oxidative damage of various molecules including DNA. This can
result in mutations, replication errors and instability of the entire host genome.
Therefore, the aim of this study was to evaluate the level of DNA damage in
leukocytes of dogs infected with D. immitis, before, during and after the application
of antiparasitic therapy, using the in vivo comet assay. Additionally, their sensitivity
to the presence of a mutagen (H2O2) was monitored in the ex vivo comet test. Whole
blood samples from 34 dogs from Serbia, aged from one to 13 years, of both sexes,
were used. Genotoxicity assessment showed that D. immitis infection leads to DNA
damage in naturally infected dogs, with the greatest DNA damage occurring in the
group of dogs with severe clinical symptoms. Treatment with ivermectin and
doxycycline reduced DNA damage in dog leukocytes in all groups, and this was
consequently due to a reduction in the intensity of infection and oxidative stress. The
results of the ex vivo comet test showed an increased sensitivity of leukocytes to DNA
damage caused by hydrogen peroxide (H2O2) before therapy, while the applied
therapy led to an increase in the leukocyte resistance to DNA damage. The obtained
results indicate that timely diagnosis and initiation of therapy is of great importance
in reducing the proven negative effects of D. immitis infection, including DNA
damage of the host.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024
T1  - Procena efekta infekcije nematodom Dirofilaria immitis i primenjene antiparazitske terapije na  stepen oštećenja DNK pasa
SP  - 36
EP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3865
ER  - 

@conference{
author = "Rajković, Milan and Glavinić, Uroš and Bogunović, Danica and Jovanović, Nemanja M. and Ristanić, Marko and Blagojević, Jovan and Stanimirović, Zoran",
year = "2024",
abstract = "Dirofilaria immitis је један од најчешћих паразита који погађа домаће и дивље
месоједе, а преносе га комарци из родова Culex, Aedes и Anopheles. Поред адулта
који су локализовани у плућним артеријама, циркулишуће микрофиларије,
такође, имају улогу у покретању инфламаторног одговора домаћина који је
кључан за патогенезу и даљи развој болести. Реактивне врсте кисеоника (ROS)
које настају током инфламаторног одговора су важан део одбрамбених
стратегија организама за елиминацију паразита, али могу довести и до
оксидативног оштећења различитих молекула укључујући и ДНК. Ово може
резултирати појавом мутација, грешака у репликацији и нестабилности читавог
генома домаћина. Стога је циљ овог истраживања била процена степена
оштећења ДНК у леукоцитима паса инфицираних врстом D. immitis, пре, током
и након примене антипаразитске терапије, употребом in vivo комет теста.
Додатно, праћена је њихова осетљивост на присуство мутагена (H2O2) у ex vivo
комет тесту. Коришћени су узорци пуне крви од 34 пса пореклом из Србије,
старости од једне до 13 година, оба пола. Процена генотоксичности је показала
да инфекција узрокована врстом D. immitis доводи до оштећења ДНК код
природно заражених паса, при чему се највећа оштећења ДНК јављају у групи
паса са тежим клиничким знацима. Третман ивермектином и доксициклином
смањио је оштећење ДНК у леукоцитима паса у свим групама и то услед
смањења интензитета инфекције и оксидативног стреса. Резултати ex vivo комет
теста показали су повећану осетљивост леукоцита на оштећење ДНК изазвано
водоокник-пероксидом (H2O2) пре терапије, док је примењена терапија довела
до повећања резистенције леукоцита на оштећење ДНК. Добијени резултати
указују да је благовремено постављање дијагнозе и отпочињање терапије од
великог значаја за смањење доказаних негативих ефеката инфекције врстом D.
immitis, укључујући и оштећење ДНК домаћина., Dirofilaria immitis is one of the most common parasites affecting domestic and wild
carnivores, and it is transmitted by mosquitoes from the genera Culex, Aedes and
Anopheles. In addition to adults localized in the pulmonary arteries, circulating
microfilariae also plays a significant role in triggering the host's inflammatory
response, which is crucial for the pathogenesis and further development of the disease.
Reactive oxygen species (ROS) generated during the inflammatory response are an
important part of the defense of the organism in the elimination of the parasites but
can also lead to oxidative damage of various molecules including DNA. This can
result in mutations, replication errors and instability of the entire host genome.
Therefore, the aim of this study was to evaluate the level of DNA damage in
leukocytes of dogs infected with D. immitis, before, during and after the application
of antiparasitic therapy, using the in vivo comet assay. Additionally, their sensitivity
to the presence of a mutagen (H2O2) was monitored in the ex vivo comet test. Whole
blood samples from 34 dogs from Serbia, aged from one to 13 years, of both sexes,
were used. Genotoxicity assessment showed that D. immitis infection leads to DNA
damage in naturally infected dogs, with the greatest DNA damage occurring in the
group of dogs with severe clinical symptoms. Treatment with ivermectin and
doxycycline reduced DNA damage in dog leukocytes in all groups, and this was
consequently due to a reduction in the intensity of infection and oxidative stress. The
results of the ex vivo comet test showed an increased sensitivity of leukocytes to DNA
damage caused by hydrogen peroxide (H2O2) before therapy, while the applied
therapy led to an increase in the leukocyte resistance to DNA damage. The obtained
results indicate that timely diagnosis and initiation of therapy is of great importance
in reducing the proven negative effects of D. immitis infection, including DNA
damage of the host.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024",
title = "Procena efekta infekcije nematodom Dirofilaria immitis i primenjene antiparazitske terapije na  stepen oštećenja DNK pasa",
pages = "36-37",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3865"
}

Rajković, M., Glavinić, U., Bogunović, D., Jovanović, N. M., Ristanić, M., Blagojević, J.,& Stanimirović, Z.. (2024). Procena efekta infekcije nematodom Dirofilaria immitis i primenjene antiparazitske terapije na  stepen oštećenja DNK pasa. in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 36-37.
https://hdl.handle.net/21.15107/rcub_veterinar_3865

Rajković M, Glavinić U, Bogunović D, Jovanović NM, Ristanić M, Blagojević J, Stanimirović Z. Procena efekta infekcije nematodom Dirofilaria immitis i primenjene antiparazitske terapije na  stepen oštećenja DNK pasa. in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024. 2024;:36-37.
https://hdl.handle.net/21.15107/rcub_veterinar_3865 .

Rajković, Milan, Glavinić, Uroš, Bogunović, Danica, Jovanović, Nemanja M., Ristanić, Marko, Blagojević, Jovan, Stanimirović, Zoran, "Procena efekta infekcije nematodom Dirofilaria immitis i primenjene antiparazitske terapije na  stepen oštećenja DNK pasa" in XXVI Simpozijum epizootiologa i epidemiologa (XXVI Epizootiološki dani), Banja Koviljača, 10 - 12. april 2024 (2024):36-37,
https://hdl.handle.net/21.15107/rcub_veterinar_3865 .

TY  - JOUR
AU  - Jovanović, Namanja M.
AU  - Despotović, Darko
AU  - Stepanović, Predrag
AU  - Rajković, Milan
AU  - Ilić, Tamara
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2948
AB  - Acanthocheilonema (Dipetalonema) reconditum is a less pathogenic species of filaria from the superfamily Filarioidea, and which parasitizes in the subcutaneous connective tissue of dogs, hyenas and jackals. The results of epidemiological studies indicate the zoonotic potential of A. reconditum, bearing in mind it can cause infections with clinical disorders in humans. This filaria is spread globally and it is mostly described in geographical areas such as the Mediterranean Basin, the Middle East, South Africa, South America and Oceania, where it is the only or is the most common filaria that infects dogs. The prevalence and distribution of A. reconditum depend mainly on the vector population, impact of environmental factors, and lifestyle of pets and their owners. Apart from the standard parasitological techniques for differential diagnostics of filariae, more attention is being dedicated to the development of protocols that are based on the simultaneous detection of specific DNA regions in each type of individual filaria. Due to its importance for public health, effective vector control is required, as well as regular preventive examinations, reliable diagnostics and therapy for A. reconditum in dogs, and continuous cooperation between veterinary and medical surgeons.
PB  - University of Belgrade, Faculty of Veterinary Medicine
T2  - Veterinarski Glasnik
T1  - Clinical-parasitological and epidemiological review of the nematode Acanthocheilonema reconditum
VL  - 77
IS  - 1
SP  - 1
EP  - 15
DO  - 10.2298/VETGL220307008J
ER  - 

@article{
author = "Jovanović, Namanja M. and Despotović, Darko and Stepanović, Predrag and Rajković, Milan and Ilić, Tamara",
year = "2023",
abstract = "Acanthocheilonema (Dipetalonema) reconditum is a less pathogenic species of filaria from the superfamily Filarioidea, and which parasitizes in the subcutaneous connective tissue of dogs, hyenas and jackals. The results of epidemiological studies indicate the zoonotic potential of A. reconditum, bearing in mind it can cause infections with clinical disorders in humans. This filaria is spread globally and it is mostly described in geographical areas such as the Mediterranean Basin, the Middle East, South Africa, South America and Oceania, where it is the only or is the most common filaria that infects dogs. The prevalence and distribution of A. reconditum depend mainly on the vector population, impact of environmental factors, and lifestyle of pets and their owners. Apart from the standard parasitological techniques for differential diagnostics of filariae, more attention is being dedicated to the development of protocols that are based on the simultaneous detection of specific DNA regions in each type of individual filaria. Due to its importance for public health, effective vector control is required, as well as regular preventive examinations, reliable diagnostics and therapy for A. reconditum in dogs, and continuous cooperation between veterinary and medical surgeons.",
publisher = "University of Belgrade, Faculty of Veterinary Medicine",
journal = "Veterinarski Glasnik",
title = "Clinical-parasitological and epidemiological review of the nematode Acanthocheilonema reconditum",
volume = "77",
number = "1",
pages = "1-15",
doi = "10.2298/VETGL220307008J"
}

Jovanović, N. M., Despotović, D., Stepanović, P., Rajković, M.,& Ilić, T.. (2023). Clinical-parasitological and epidemiological review of the nematode Acanthocheilonema reconditum. in Veterinarski Glasnik
University of Belgrade, Faculty of Veterinary Medicine., 77(1), 1-15.
https://doi.org/10.2298/VETGL220307008J

Jovanović NM, Despotović D, Stepanović P, Rajković M, Ilić T. Clinical-parasitological and epidemiological review of the nematode Acanthocheilonema reconditum. in Veterinarski Glasnik. 2023;77(1):1-15.
doi:10.2298/VETGL220307008J .

Jovanović, Namanja M., Despotović, Darko, Stepanović, Predrag, Rajković, Milan, Ilić, Tamara, "Clinical-parasitological and epidemiological review of the nematode Acanthocheilonema reconditum" in Veterinarski Glasnik, 77, no. 1 (2023):1-15,
https://doi.org/10.2298/VETGL220307008J . .

TY  - GEN
AU  - Bogunović, Danica
AU  - Aleksić, Nevenka
AU  - Ilić, Tamara
AU  - Jovanović, Nemanja
AU  - Rajković, Milan
AU  - Kulišić, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3091
AB  - Kućni ljubimci danas često poprimaju status člana porodice, a
interakcije vlasnika i životinja su prisnije nego što su bile u prošlosti.
Međutim, različite studije pokazale su da vlasnici nisu dovoljno informisani
o postojanju infekcija uzrokovanih parazitima. Koncept „Jedno
zdravlje” podrazumeva multidisciplinarno delovanje na tri nivoa – očuvanje
zdravlja ljudi i životinja i očuvanje životne sredine. U vezi sa time,
značajno je što većina vrsta parazita ima veoma otporne razvojne
oblike u spoljašnjoj sredini, koja predstavlja izvor infekcije za životinje
i/ili ljude. Zbog velike gustine naseljenosti i ograničenog prostora, problem
kontaminacije parazitima i/ili njihovim preparazitskim stadijumima
naročito je izražen u gradskim sredinama. Sa druge strane, u ruralnim
oblastima javljaju se parazitske infekcije u čijem ciklusu razvoja
učestvuju domaće životinje koje se gaje radi proizvodnje mesa, čovek
i pas. Rizik od infekcije zavisi od vrste parazita, njegove otpornosti u
spoljašnjoj sredini, puteva prenošenja, načina držanja životinja i sprovođenja
preventivnih i zdravstvenih mera. Problem u svakodnevnoj
praksi predstavlja i nepravilna primena antiparazitika. Ovakvi postupci
doprinose razvoju rezistencije na antihelmintike i stvaraju rizik od održavanja
i širenja infekcija, prvenstveno protozoarne etiologije, na koje
ovi preparati ne deluju. Kako bi se u potpunosti očuvao koncept „Jednog
zdravlja”, veterinari imaju ključnu ulogu u edukaciji vlasnika o potencijalnim
rizicima, pravilnom sprovođenju prevencije i zdravstvene
zaštite ljubimaca, ali i o odgovornom vlasništvu.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
T2  - XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023
T1  - Kućni ljubimci i paraziti u kontekstu jednog zdravlja
SP  - 15
EP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3091
ER  - 

@misc{
author = "Bogunović, Danica and Aleksić, Nevenka and Ilić, Tamara and Jovanović, Nemanja and Rajković, Milan and Kulišić, Zoran",
year = "2023",
abstract = "Kućni ljubimci danas često poprimaju status člana porodice, a
interakcije vlasnika i životinja su prisnije nego što su bile u prošlosti.
Međutim, različite studije pokazale su da vlasnici nisu dovoljno informisani
o postojanju infekcija uzrokovanih parazitima. Koncept „Jedno
zdravlje” podrazumeva multidisciplinarno delovanje na tri nivoa – očuvanje
zdravlja ljudi i životinja i očuvanje životne sredine. U vezi sa time,
značajno je što većina vrsta parazita ima veoma otporne razvojne
oblike u spoljašnjoj sredini, koja predstavlja izvor infekcije za životinje
i/ili ljude. Zbog velike gustine naseljenosti i ograničenog prostora, problem
kontaminacije parazitima i/ili njihovim preparazitskim stadijumima
naročito je izražen u gradskim sredinama. Sa druge strane, u ruralnim
oblastima javljaju se parazitske infekcije u čijem ciklusu razvoja
učestvuju domaće životinje koje se gaje radi proizvodnje mesa, čovek
i pas. Rizik od infekcije zavisi od vrste parazita, njegove otpornosti u
spoljašnjoj sredini, puteva prenošenja, načina držanja životinja i sprovođenja
preventivnih i zdravstvenih mera. Problem u svakodnevnoj
praksi predstavlja i nepravilna primena antiparazitika. Ovakvi postupci
doprinose razvoju rezistencije na antihelmintike i stvaraju rizik od održavanja
i širenja infekcija, prvenstveno protozoarne etiologije, na koje
ovi preparati ne deluju. Kako bi se u potpunosti očuvao koncept „Jednog
zdravlja”, veterinari imaju ključnu ulogu u edukaciji vlasnika o potencijalnim
rizicima, pravilnom sprovođenju prevencije i zdravstvene
zaštite ljubimaca, ali i o odgovornom vlasništvu.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023",
title = "Kućni ljubimci i paraziti u kontekstu jednog zdravlja",
pages = "15-30",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3091"
}

Bogunović, D., Aleksić, N., Ilić, T., Jovanović, N., Rajković, M.,& Kulišić, Z.. (2023). Kućni ljubimci i paraziti u kontekstu jednog zdravlja. in XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 15-30.
https://hdl.handle.net/21.15107/rcub_veterinar_3091

Bogunović D, Aleksić N, Ilić T, Jovanović N, Rajković M, Kulišić Z. Kućni ljubimci i paraziti u kontekstu jednog zdravlja. in XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023. 2023;:15-30.
https://hdl.handle.net/21.15107/rcub_veterinar_3091 .

Bogunović, Danica, Aleksić, Nevenka, Ilić, Tamara, Jovanović, Nemanja, Rajković, Milan, Kulišić, Zoran, "Kućni ljubimci i paraziti u kontekstu jednog zdravlja" in XLIV seminar za inovacije znanja veterinara, Beograd, 24. februar 2023 (2023):15-30,
https://hdl.handle.net/21.15107/rcub_veterinar_3091 .

TY  - JOUR
AU  - Glavinić, Uroš
AU  - Rajković, Milan
AU  - Ristanić, Marko
AU  - Stevanović, Jevrosima
AU  - Vejnović, Branislav
AU  - Đelić, Ninoslav
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2942
AB  - Thymol is a natural essential oil derived from the plant Thymus vulgaris L. It is known to be beneficial for human and animal health and has been used in beekeeping practice against Varroa mite for years. In this study, the genotoxic and antigenotoxic potential of thymol were evaluated on the honey bee (Apis mellifera L.) continuous cell line AmE-711 for the first time. Using the Comet assay, three increasing concentrations (10, 100, and 1000 µg/mL) of thymol were tested. Negative control (non-treated cells) and positive control (cells treated with 100 µM H2O2) were also included. The absence of thymol cytotoxicity was confirmed with the Trypan blue exclusion test. Thymol in the concentration of 10 µg/mL did not increase DNA damage in AmE-711 honey bee cells, while 100 and 1000 µg/mL concentrations showed genotoxic effects. For testing the antigenotoxic effect, all concentrations of thymol were mixed and incubated with H2O2. The antigenotoxic effect against was absent at all concentrations (10, 100, 1000 μg/mL) tested. Moreover, thymol enhanced the H2O2-induced DNA migration in the Comet assay. The obtained results indicate genotoxic effects of thymol on cultured honey bee cells suggesting its careful application in beekeeping practice to avoid possible negative effects on honey bees. © 2023 by the authors.
PB  - MDPI
T2  - Insects
T1  - Genotoxic Potential of Thymol on Honey Bee DNA in the Comet Assay
VL  - 14
IS  - 5
SP  - 451
DO  - 10.3390/insects14050451
ER  - 

@article{
author = "Glavinić, Uroš and Rajković, Milan and Ristanić, Marko and Stevanović, Jevrosima and Vejnović, Branislav and Đelić, Ninoslav and Stanimirović, Zoran",
year = "2023",
abstract = "Thymol is a natural essential oil derived from the plant Thymus vulgaris L. It is known to be beneficial for human and animal health and has been used in beekeeping practice against Varroa mite for years. In this study, the genotoxic and antigenotoxic potential of thymol were evaluated on the honey bee (Apis mellifera L.) continuous cell line AmE-711 for the first time. Using the Comet assay, three increasing concentrations (10, 100, and 1000 µg/mL) of thymol were tested. Negative control (non-treated cells) and positive control (cells treated with 100 µM H2O2) were also included. The absence of thymol cytotoxicity was confirmed with the Trypan blue exclusion test. Thymol in the concentration of 10 µg/mL did not increase DNA damage in AmE-711 honey bee cells, while 100 and 1000 µg/mL concentrations showed genotoxic effects. For testing the antigenotoxic effect, all concentrations of thymol were mixed and incubated with H2O2. The antigenotoxic effect against was absent at all concentrations (10, 100, 1000 μg/mL) tested. Moreover, thymol enhanced the H2O2-induced DNA migration in the Comet assay. The obtained results indicate genotoxic effects of thymol on cultured honey bee cells suggesting its careful application in beekeeping practice to avoid possible negative effects on honey bees. © 2023 by the authors.",
publisher = "MDPI",
journal = "Insects",
title = "Genotoxic Potential of Thymol on Honey Bee DNA in the Comet Assay",
volume = "14",
number = "5",
pages = "451",
doi = "10.3390/insects14050451"
}

Glavinić, U., Rajković, M., Ristanić, M., Stevanović, J., Vejnović, B., Đelić, N.,& Stanimirović, Z.. (2023). Genotoxic Potential of Thymol on Honey Bee DNA in the Comet Assay. in Insects
MDPI., 14(5), 451.
https://doi.org/10.3390/insects14050451

Glavinić U, Rajković M, Ristanić M, Stevanović J, Vejnović B, Đelić N, Stanimirović Z. Genotoxic Potential of Thymol on Honey Bee DNA in the Comet Assay. in Insects. 2023;14(5):451.
doi:10.3390/insects14050451 .

Glavinić, Uroš, Rajković, Milan, Ristanić, Marko, Stevanović, Jevrosima, Vejnović, Branislav, Đelić, Ninoslav, Stanimirović, Zoran, "Genotoxic Potential of Thymol on Honey Bee DNA in the Comet Assay" in Insects, 14, no. 5 (2023):451,
https://doi.org/10.3390/insects14050451 . .

TY  - JOUR
AU  - Ristanić, Marko
AU  - Vučković, Jelena
AU  - Dominiković, Nina
AU  - Vučićević, Miloš
AU  - Rajković, Milan
AU  - Bogunović, Danica
AU  - Özvegy, József
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3140
AB  - Protozoa of the genus Giardia cause the disease giardiosis in a large number of animal
species, but also in humans. This disease can be either subclinical or followed by diarrhea,
malabsorption, weight loss and stunted growth. The aim of this study was to examine the
presence of Giardia sp. in different species of animals in Belgrade Zoo (asymptomatic
infection) and to compare the sensitivity of parasitological and molecular-genetic
methods. Examination of the presence of Giardia sp. protozoa was performed in 52
fecal samples, using fecal fl otation and molecular-genetic PCR method. No positive
samples were detected when deploying the parasitological method, while PRC analysis
revealed the presence of Giardia sp. in one animal - ring-tailed lemur (Lemur catta),
which indicates the advantage of the molecular-genetic method over the parasitological
one. The absence of Giardia sp. in other samples can be explained by adequate housing
conditions, successful implementation of hygienic and sanitary measures, as well as
good veterinary practice. Use of PCR method for testing the presence of Giardia sp.
showed a higher sensitivity when compared to the fecal fl otation method.
PB  - Beograd : Fakultet veterinarske medicine
T2  - Acta Veterinaria
T1  - Use of parasitological and molecular methods in Giardia sp. detection in animals held in captivity
VL  - 73
IS  - 2
SP  - 195
EP  - 204
DO  - 10.2478/acve-2023-0015
ER  - 

@article{
author = "Ristanić, Marko and Vučković, Jelena and Dominiković, Nina and Vučićević, Miloš and Rajković, Milan and Bogunović, Danica and Özvegy, József",
year = "2023",
abstract = "Protozoa of the genus Giardia cause the disease giardiosis in a large number of animal
species, but also in humans. This disease can be either subclinical or followed by diarrhea,
malabsorption, weight loss and stunted growth. The aim of this study was to examine the
presence of Giardia sp. in different species of animals in Belgrade Zoo (asymptomatic
infection) and to compare the sensitivity of parasitological and molecular-genetic
methods. Examination of the presence of Giardia sp. protozoa was performed in 52
fecal samples, using fecal fl otation and molecular-genetic PCR method. No positive
samples were detected when deploying the parasitological method, while PRC analysis
revealed the presence of Giardia sp. in one animal - ring-tailed lemur (Lemur catta),
which indicates the advantage of the molecular-genetic method over the parasitological
one. The absence of Giardia sp. in other samples can be explained by adequate housing
conditions, successful implementation of hygienic and sanitary measures, as well as
good veterinary practice. Use of PCR method for testing the presence of Giardia sp.
showed a higher sensitivity when compared to the fecal fl otation method.",
publisher = "Beograd : Fakultet veterinarske medicine",
journal = "Acta Veterinaria",
title = "Use of parasitological and molecular methods in Giardia sp. detection in animals held in captivity",
volume = "73",
number = "2",
pages = "195-204",
doi = "10.2478/acve-2023-0015"
}

Ristanić, M., Vučković, J., Dominiković, N., Vučićević, M., Rajković, M., Bogunović, D.,& Özvegy, J.. (2023). Use of parasitological and molecular methods in Giardia sp. detection in animals held in captivity. in Acta Veterinaria
Beograd : Fakultet veterinarske medicine., 73(2), 195-204.
https://doi.org/10.2478/acve-2023-0015

Ristanić M, Vučković J, Dominiković N, Vučićević M, Rajković M, Bogunović D, Özvegy J. Use of parasitological and molecular methods in Giardia sp. detection in animals held in captivity. in Acta Veterinaria. 2023;73(2):195-204.
doi:10.2478/acve-2023-0015 .

Ristanić, Marko, Vučković, Jelena, Dominiković, Nina, Vučićević, Miloš, Rajković, Milan, Bogunović, Danica, Özvegy, József, "Use of parasitological and molecular methods in Giardia sp. detection in animals held in captivity" in Acta Veterinaria, 73, no. 2 (2023):195-204,
https://doi.org/10.2478/acve-2023-0015 . .

TY  - JOUR
AU  - Rajković, Milan
AU  - Glavinić, Uroš
AU  - Bogunović, Danica
AU  - Vejnović, Branislav
AU  - Davitkov, Darko
AU  - Đelić, Ninoslav
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3191
AB  - Parasitic diseases are considered to be a cause of oxidative stress which leads to oxidative damage of various
molecules including DNA. This can result in mutations, replication errors, and genome instability. Therefore, aim
of this study was to measure DNA damage induced by Dirofilaria immitis in the single cells such as dogs’ leukocytes using the comet assay. Also, we monitored the effects of antiparasitic treatment on mitigation of
sensitivity to DNA damage in leukocytes treated with H2O2 using the in vivo and ex vivo comet assay. The whole
blood samples from 34 dogs from Serbia were used, both males and females, from one to 13 years old, both pure
and mixed-breeds. A rapid immunochromatographic test (Antigen Rapid Heartworm Ag 2.0 Test Kit, Bionote,
Minnesota, USA) was used for the detection of D. immitis antigens. The modified Knott’s test and PCR were used
in the aim of detecting D. immitis microfilariae in dogs’ blood, and evaluating the number of circulating
microfilariae during the treatment. The genotoxicity evaluation showed that D. immitis infection resulted in DNA
damage in naturally infected dogs, with the highest DNA damage occurring in the group of dogs with severe
clinical signs. Treatment with ivermectin and doxycycline decreased DNA damage in leukocytes of dogs in all
groups, as the intensity of infection decreased due to applied therapy. Ex vivo comet assay results showed that
leukocytes exhibited decreased sensitivity to H2O2-induced DNA damage during treatment. The results of the
modified Knott’s test and PCR in our study showed that treatment with ivermectin and doxycycline was successful in decreasing the average number of microfilariae during the time and at the end eliminating them from
the dogs’ blood.
PB  - Elsevier
T2  - Veterinary Parasitology
T1  - “Slow kill” treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis
VL  - 322
SP  - 110008
DO  - 10.1016/j.vetpar.2023.110008
ER  - 

@article{
author = "Rajković, Milan and Glavinić, Uroš and Bogunović, Danica and Vejnović, Branislav and Davitkov, Darko and Đelić, Ninoslav and Stanimirović, Zoran",
year = "2023",
abstract = "Parasitic diseases are considered to be a cause of oxidative stress which leads to oxidative damage of various
molecules including DNA. This can result in mutations, replication errors, and genome instability. Therefore, aim
of this study was to measure DNA damage induced by Dirofilaria immitis in the single cells such as dogs’ leukocytes using the comet assay. Also, we monitored the effects of antiparasitic treatment on mitigation of
sensitivity to DNA damage in leukocytes treated with H2O2 using the in vivo and ex vivo comet assay. The whole
blood samples from 34 dogs from Serbia were used, both males and females, from one to 13 years old, both pure
and mixed-breeds. A rapid immunochromatographic test (Antigen Rapid Heartworm Ag 2.0 Test Kit, Bionote,
Minnesota, USA) was used for the detection of D. immitis antigens. The modified Knott’s test and PCR were used
in the aim of detecting D. immitis microfilariae in dogs’ blood, and evaluating the number of circulating
microfilariae during the treatment. The genotoxicity evaluation showed that D. immitis infection resulted in DNA
damage in naturally infected dogs, with the highest DNA damage occurring in the group of dogs with severe
clinical signs. Treatment with ivermectin and doxycycline decreased DNA damage in leukocytes of dogs in all
groups, as the intensity of infection decreased due to applied therapy. Ex vivo comet assay results showed that
leukocytes exhibited decreased sensitivity to H2O2-induced DNA damage during treatment. The results of the
modified Knott’s test and PCR in our study showed that treatment with ivermectin and doxycycline was successful in decreasing the average number of microfilariae during the time and at the end eliminating them from
the dogs’ blood.",
publisher = "Elsevier",
journal = "Veterinary Parasitology",
title = "“Slow kill” treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis",
volume = "322",
pages = "110008",
doi = "10.1016/j.vetpar.2023.110008"
}

Rajković, M., Glavinić, U., Bogunović, D., Vejnović, B., Davitkov, D., Đelić, N.,& Stanimirović, Z.. (2023). “Slow kill” treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis. in Veterinary Parasitology
Elsevier., 322, 110008.
https://doi.org/10.1016/j.vetpar.2023.110008

Rajković M, Glavinić U, Bogunović D, Vejnović B, Davitkov D, Đelić N, Stanimirović Z. “Slow kill” treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis. in Veterinary Parasitology. 2023;322:110008.
doi:10.1016/j.vetpar.2023.110008 .

Rajković, Milan, Glavinić, Uroš, Bogunović, Danica, Vejnović, Branislav, Davitkov, Darko, Đelić, Ninoslav, Stanimirović, Zoran, "“Slow kill” treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis" in Veterinary Parasitology, 322 (2023):110008,
https://doi.org/10.1016/j.vetpar.2023.110008 . .

TY  - CONF
AU  - Jovanović, Nemanja M.
AU  - Glavinić, Uroš
AU  - Rajković, Milan
AU  - Stevanović, Jevrosima
AU  - Ristanić, Marko
AU  - Ilić, Tamara
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3031
AB  - Један од водећих узрока губитака пчелињих заједница је неадекватна исхрана у
погледу квалитета, квантитета и разноврсности, што води повећању осетљивости
на патогене и пестициде. Одузимањем превелике количине меда и прихрана пчела
са чистим шећерним сирупом изазива енергетски и оксидативни стрес што
последично води ка повећаној осетљивости пчела на патогене, првенствено на
ендопаразита Nosema ceranae. У одсуству адекватне хране у пчелињим
заједницама, често се користе суплементи, чиме се може повећати отпорност пчела
према патогенима и одржавати добро кондиционо стање пчелињих заједница. Циљ
овог истраживања је био испитивање ефеката биљног суплемента (комерцијални
назив B+) у кошничким условима, анализирајући параметре јачине пчелињих
заједница (површина отвореног и затвореног легла, резерве меда и полена и број
адултних пчела) и количину ендопаразита N. ceranae. Експеримент је изведен у две
фазе, прва фаза у августу 2018. године и друга фаза у марту 2019. године.
Поређењем праћених параметара на крају прве фазе, уочено је повећање (p < 0,05)
резерви меда и полена и броја адултних пчела у заједницама прихрањиваним
суплементом B+. Током друге фазе, вредности свих посматраних параметара су
биле значајно више (p < 0,05) у заједницама које су у исхрани користиле суплемент
B+ у односу на оне које суплемент нису добијале. Додатно, број спора N. ceranae је
током експеримента био значајно нижи (p < 0,05) у заједницама прихрањиваним
суплементом B+ у односу на оне храњене без додатка суплемента. На основу
добијених резултата утврђени су позитивни ефекти код заједница које су кроз
исхрану користиле тестирани суплемент јер се на тај начин обезбеђују хранљиви
елементи којих нема у чистом шећерном сирупу. Коришћењем овог суплемента у
јесењој прихрани пчела превенира се развој микроспоридије N. ceranae, чиме се
пчелиње заједнице знатно боље зазимљавају. У пролећној прихрани, постиже се
боља припремљеност заједница за главну пашу и побољшање производних и
репродуктивних параметара пчелињих заједница
AB  - One of the main causes of bee colony losses is inadequate nutrition in terms of quality,
quantity and diversity, which leads to increased susceptibility to pathogens and
pesticides. Taking too much honey and feeding bees with pure saccharose syrup
causes energetic and oxidative stress, which in turn leads to increased sensitivity of
bees to pathogens, primarily the endoparasite Nosema ceranae. In the absence of
adequate food in bee colonies, supplements are often used, which can increase the
resistance of bees to pathogens and maintain good health condition of bee colonies.
The aim of this study was to examine the effects of herbal supplement (commercial
name B+) in hive conditions, analysing the colonies strength parameters (open and
sealed brood area; honey and pollen reserves and number of adult bees) and level of
N. ceranae. The experiment was conducted in two phases; the first phase was
conducted in August 2018, while the second phase was conducted in March 2019. An
increase (p <0.05) of honey and pollen reserves and the number of adult bees in the
colonies fed with B + supplement was noticed, when the examined parameters were
compared at the end of the first phase. In the second phase, the values of all observed
parameters were significantly higher (p <0.05) in the colonies that used the B+
supplement in their diet, compared to those that did not receive the supplement. In
addition, the number of N. ceranae spores was significantly lower (p <0.05) during
the experiment in colonies fed with B+ supplement, compared to those which were
fed without supplement. Based on the obtained results, positive effects were
determined in colonies that used the tested supplement through their diet, because the
supplement provides nutrients that are not present in pure saccharose syrup. The use
of this supplement in the autumn, prevents the development of microsporidia N.
ceranae, which helps in better wintering of bee colonies. Moreover, application of the
B+ supplement in the spring, provides better preparation of colonies for the main
grazing and improvement of production and reproductive parameters of bee colonies.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022
T1  - Suplement koji sadrži B-kompleks vitamina može da poboljša zdravlje pčela i proizvodne performanse zajednica
T1  - Supplement containing B-complex vitamins can improve bee health and increase colony performance
IS  - 140
EP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3031
ER  - 

@conference{
author = "Jovanović, Nemanja M. and Glavinić, Uroš and Rajković, Milan and Stevanović, Jevrosima and Ristanić, Marko and Ilić, Tamara and Stanimirović, Zoran",
year = "2022",
abstract = "Један од водећих узрока губитака пчелињих заједница је неадекватна исхрана у
погледу квалитета, квантитета и разноврсности, што води повећању осетљивости
на патогене и пестициде. Одузимањем превелике количине меда и прихрана пчела
са чистим шећерним сирупом изазива енергетски и оксидативни стрес што
последично води ка повећаној осетљивости пчела на патогене, првенствено на
ендопаразита Nosema ceranae. У одсуству адекватне хране у пчелињим
заједницама, често се користе суплементи, чиме се може повећати отпорност пчела
према патогенима и одржавати добро кондиционо стање пчелињих заједница. Циљ
овог истраживања је био испитивање ефеката биљног суплемента (комерцијални
назив B+) у кошничким условима, анализирајући параметре јачине пчелињих
заједница (површина отвореног и затвореног легла, резерве меда и полена и број
адултних пчела) и количину ендопаразита N. ceranae. Експеримент је изведен у две
фазе, прва фаза у августу 2018. године и друга фаза у марту 2019. године.
Поређењем праћених параметара на крају прве фазе, уочено је повећање (p < 0,05)
резерви меда и полена и броја адултних пчела у заједницама прихрањиваним
суплементом B+. Током друге фазе, вредности свих посматраних параметара су
биле значајно више (p < 0,05) у заједницама које су у исхрани користиле суплемент
B+ у односу на оне које суплемент нису добијале. Додатно, број спора N. ceranae је
током експеримента био значајно нижи (p < 0,05) у заједницама прихрањиваним
суплементом B+ у односу на оне храњене без додатка суплемента. На основу
добијених резултата утврђени су позитивни ефекти код заједница које су кроз
исхрану користиле тестирани суплемент јер се на тај начин обезбеђују хранљиви
елементи којих нема у чистом шећерном сирупу. Коришћењем овог суплемента у
јесењој прихрани пчела превенира се развој микроспоридије N. ceranae, чиме се
пчелиње заједнице знатно боље зазимљавају. У пролећној прихрани, постиже се
боља припремљеност заједница за главну пашу и побољшање производних и
репродуктивних параметара пчелињих заједница, One of the main causes of bee colony losses is inadequate nutrition in terms of quality,
quantity and diversity, which leads to increased susceptibility to pathogens and
pesticides. Taking too much honey and feeding bees with pure saccharose syrup
causes energetic and oxidative stress, which in turn leads to increased sensitivity of
bees to pathogens, primarily the endoparasite Nosema ceranae. In the absence of
adequate food in bee colonies, supplements are often used, which can increase the
resistance of bees to pathogens and maintain good health condition of bee colonies.
The aim of this study was to examine the effects of herbal supplement (commercial
name B+) in hive conditions, analysing the colonies strength parameters (open and
sealed brood area; honey and pollen reserves and number of adult bees) and level of
N. ceranae. The experiment was conducted in two phases; the first phase was
conducted in August 2018, while the second phase was conducted in March 2019. An
increase (p <0.05) of honey and pollen reserves and the number of adult bees in the
colonies fed with B + supplement was noticed, when the examined parameters were
compared at the end of the first phase. In the second phase, the values of all observed
parameters were significantly higher (p <0.05) in the colonies that used the B+
supplement in their diet, compared to those that did not receive the supplement. In
addition, the number of N. ceranae spores was significantly lower (p <0.05) during
the experiment in colonies fed with B+ supplement, compared to those which were
fed without supplement. Based on the obtained results, positive effects were
determined in colonies that used the tested supplement through their diet, because the
supplement provides nutrients that are not present in pure saccharose syrup. The use
of this supplement in the autumn, prevents the development of microsporidia N.
ceranae, which helps in better wintering of bee colonies. Moreover, application of the
B+ supplement in the spring, provides better preparation of colonies for the main
grazing and improvement of production and reproductive parameters of bee colonies.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022",
title = "Suplement koji sadrži B-kompleks vitamina može da poboljša zdravlje pčela i proizvodne performanse zajednica, Supplement containing B-complex vitamins can improve bee health and increase colony performance",
number = "140",
pages = "141",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3031"
}

Jovanović, N. M., Glavinić, U., Rajković, M., Stevanović, J., Ristanić, M., Ilić, T.,& Stanimirović, Z.. (2022). Suplement koji sadrži B-kompleks vitamina može da poboljša zdravlje pčela i proizvodne performanse zajednica. in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze.(140).
https://hdl.handle.net/21.15107/rcub_veterinar_3031

Jovanović NM, Glavinić U, Rajković M, Stevanović J, Ristanić M, Ilić T, Stanimirović Z. Suplement koji sadrži B-kompleks vitamina može da poboljša zdravlje pčela i proizvodne performanse zajednica. in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022. 2022;(140):null-141.
https://hdl.handle.net/21.15107/rcub_veterinar_3031 .

Jovanović, Nemanja M., Glavinić, Uroš, Rajković, Milan, Stevanović, Jevrosima, Ristanić, Marko, Ilić, Tamara, Stanimirović, Zoran, "Suplement koji sadrži B-kompleks vitamina može da poboljša zdravlje pčela i proizvodne performanse zajednica" in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022, no. 140 (2022),
https://hdl.handle.net/21.15107/rcub_veterinar_3031 .

TY  - CONF
AU  - Glavinić, Uroš
AU  - Ristanić, Marko
AU  - Jovanović, Nemanja M.
AU  - Rajković, Milan
AU  - Niketić, Mia
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2419
AB  - donosne pčele (Apis mellifera). Izaziva bolest nozemozu (nozematozu) i u zavisnosti od stepena infekcije i jačine pčelinjih društava,
ostavlja značajne posledice na zdravlje, reproduktivne i produktivne
sposobnosti pčela.
Antibiotik fumagilin je godinama bio jedini lek dostupan za kontrolu ovog patogena. Njegova primena dovedena je u pitanje zbog
nedostataka koji prate njegovu primenu (efikasnost, mortalitet pčela i pojava rezidua u pčelinjim proizvodima). Širom sveta se sprovode istraživanja sa ciljem pronalaska alternative za kontrolu nozeme.
U ovom radu prikazani su naši rezultati ispitivanja dijetetskih suplemenata na bazi prirodnih/biljnih ekstrakata (Medenko forte i ekstrakti gljiva Agaricus blazei i A. bisporus), timola i vitaminsko-mineralnog
aditiva (BEEWELL AminoPlus). Kod pčela tretiranih svim nabrojanim
suplementima i fumagilinom kao kontrolnim tretmanom, praćen je broj
spora N. ceranae, nivoi ekspresije gena značajnih za imunitet pčela
kao i parametri oksidativnog stresa. Svi preparati primenjivani su preventivno (tri dana pre infekcije), u trenutku inficiranja, kao i tri dana nakon infekcije sa N. ceranae, u cilju određivanja najboljeg momenta za
primenu. Dokazani su negativni efekti fumagilina kada je preventivno
primenjivan kod neinficiranih pčela. Kod inficiranih pčela većina testiranih suplemenata dovela je do smanjenja broja spora N. ceranae, a
pokazali su i zaštitni efekat u smislu sprečavanja imunosupresije i oksidativnog stresa.
AB  - The microsporidian Nosema ceranae is an obligate intestinal parasite of the honey
bee (Apis mellifera). It causes nosemosis and, depending on the infection level and
the strength of the infected bee colonies, causes significant health disorders, including
malfunction of reproductive and productive capabilities of bees. The antibiotic fumagillin
was the only drug available for Nosema control for many years. However, use of fumagillin
has been questioned due to the drawbacks related to its efficacy, bee mortality and the
presence of residues in bee products. Studies aimed to find alternatives for Nosema control
are being conducted worldwide. In this paper, our results related to diet supplements based
on natural/plant extracts (Medenko forte and extracts of Agaricus blazei and A. bisporus),
thymol and vitamin-mineral additive (BEEWELL AminoPlus) are presented. Bees were
treated with these supplements and fumagillin (control treatment) and gene expression
levels (important for bee immunity), as well as oxidative stress parameters were assessed.
In order to determine adequate time for application, all supplements were applied
preventively (three days before infection), at the time of infection, as well as three days after
N. ceranae infection. In uninfected bees, fumagillin demonstrated adverse effects when
applied prophylactically. In Nosema-infected bees, tested supplements mainly contributed
to the decrease of N. ceranae spore levels, but also exhibited a protective effect based on
level of immune-related gene expression and parameters of oxidative stress.
PB  - Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - Zbornik radova - XLIII seminar inovacija znanja veterinara
T1  - Novi trendovi u kontroli Nosema ceranae
T1  - Advances in Nosema ceranae control
SP  - 79
EP  - 91
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2419
ER  - 

@conference{
author = "Glavinić, Uroš and Ristanić, Marko and Jovanović, Nemanja M. and Rajković, Milan and Niketić, Mia and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2022",
abstract = "donosne pčele (Apis mellifera). Izaziva bolest nozemozu (nozematozu) i u zavisnosti od stepena infekcije i jačine pčelinjih društava,
ostavlja značajne posledice na zdravlje, reproduktivne i produktivne
sposobnosti pčela.
Antibiotik fumagilin je godinama bio jedini lek dostupan za kontrolu ovog patogena. Njegova primena dovedena je u pitanje zbog
nedostataka koji prate njegovu primenu (efikasnost, mortalitet pčela i pojava rezidua u pčelinjim proizvodima). Širom sveta se sprovode istraživanja sa ciljem pronalaska alternative za kontrolu nozeme.
U ovom radu prikazani su naši rezultati ispitivanja dijetetskih suplemenata na bazi prirodnih/biljnih ekstrakata (Medenko forte i ekstrakti gljiva Agaricus blazei i A. bisporus), timola i vitaminsko-mineralnog
aditiva (BEEWELL AminoPlus). Kod pčela tretiranih svim nabrojanim
suplementima i fumagilinom kao kontrolnim tretmanom, praćen je broj
spora N. ceranae, nivoi ekspresije gena značajnih za imunitet pčela
kao i parametri oksidativnog stresa. Svi preparati primenjivani su preventivno (tri dana pre infekcije), u trenutku inficiranja, kao i tri dana nakon infekcije sa N. ceranae, u cilju određivanja najboljeg momenta za
primenu. Dokazani su negativni efekti fumagilina kada je preventivno
primenjivan kod neinficiranih pčela. Kod inficiranih pčela većina testiranih suplemenata dovela je do smanjenja broja spora N. ceranae, a
pokazali su i zaštitni efekat u smislu sprečavanja imunosupresije i oksidativnog stresa., The microsporidian Nosema ceranae is an obligate intestinal parasite of the honey
bee (Apis mellifera). It causes nosemosis and, depending on the infection level and
the strength of the infected bee colonies, causes significant health disorders, including
malfunction of reproductive and productive capabilities of bees. The antibiotic fumagillin
was the only drug available for Nosema control for many years. However, use of fumagillin
has been questioned due to the drawbacks related to its efficacy, bee mortality and the
presence of residues in bee products. Studies aimed to find alternatives for Nosema control
are being conducted worldwide. In this paper, our results related to diet supplements based
on natural/plant extracts (Medenko forte and extracts of Agaricus blazei and A. bisporus),
thymol and vitamin-mineral additive (BEEWELL AminoPlus) are presented. Bees were
treated with these supplements and fumagillin (control treatment) and gene expression
levels (important for bee immunity), as well as oxidative stress parameters were assessed.
In order to determine adequate time for application, all supplements were applied
preventively (three days before infection), at the time of infection, as well as three days after
N. ceranae infection. In uninfected bees, fumagillin demonstrated adverse effects when
applied prophylactically. In Nosema-infected bees, tested supplements mainly contributed
to the decrease of N. ceranae spore levels, but also exhibited a protective effect based on
level of immune-related gene expression and parameters of oxidative stress.",
publisher = "Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "Zbornik radova - XLIII seminar inovacija znanja veterinara",
title = "Novi trendovi u kontroli Nosema ceranae, Advances in Nosema ceranae control",
pages = "79-91",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2419"
}

Glavinić, U., Ristanić, M., Jovanović, N. M., Rajković, M., Niketić, M., Stevanović, J.,& Stanimirović, Z.. (2022). Novi trendovi u kontroli Nosema ceranae. in Zbornik radova - XLIII seminar inovacija znanja veterinara
Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 79-91.
https://hdl.handle.net/21.15107/rcub_veterinar_2419

Glavinić U, Ristanić M, Jovanović NM, Rajković M, Niketić M, Stevanović J, Stanimirović Z. Novi trendovi u kontroli Nosema ceranae. in Zbornik radova - XLIII seminar inovacija znanja veterinara. 2022;:79-91.
https://hdl.handle.net/21.15107/rcub_veterinar_2419 .

Glavinić, Uroš, Ristanić, Marko, Jovanović, Nemanja M., Rajković, Milan, Niketić, Mia, Stevanović, Jevrosima, Stanimirović, Zoran, "Novi trendovi u kontroli Nosema ceranae" in Zbornik radova - XLIII seminar inovacija znanja veterinara (2022):79-91,
https://hdl.handle.net/21.15107/rcub_veterinar_2419 .

TY  - JOUR
AU  - Ristanić, Marko
AU  - Nikšić, Aleksandar
AU  - Niketić, Mia
AU  - Jelisić, Stefan
AU  - Rajković, Milan
AU  - Glavinić, Uroš
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2480
AB  - Following the “one health” principle, we have conducted optimization of a protocol for β-casein genotyping in cattle in order to select cows with exclusively the A2A2 genotype. Gastrointestinal proteolysis of A1 β-casein in humans releases beta-casomorphin 7, which is believed to cause a number of diseases/conditions (diabetes mellitus type 1, ischemic heart disease, atherosclerosis, sudden infant death syndrome, autism, schizophrenia, gastrointestinal discomfort, and prolonged gastrointestinal passage time). On the contrary, A2 β-casein does not cause similar effects on human health, due to its different metabolism. DNA extraction was conducted from blood samples belonging to the laboratory archive of the Department of Biology, Faculty of Veterinary Medicine, University of Belgrade. Determination of genotypes was performed using the Allele Specific Polymerase Chain Reaction (AS-PCR) method. The amplification was preceded by determination of proper primer annealing temperature (65.50 °C), in order to ensure optimal genotyping results. The results obtained indicated a higher frequency of the A2 allele (0.56) compared to the A1 allele (0.44). Furthermore, in 7 out of 35 tested samples, the A1A1 genotype (20.00%) was found, in 17 samples, the A1A2 genotype (48.60%) was found, and in 11 samples, the A2A2 genotype (31.40%) was found. The molecular methods used ensured reliable β-casein genotyping that would enable selection of cows with the A2A2 β-casein genotype, implying production of.
AB  - Kazeini čine 80% ukupnih proteina mleka, dok β-kazein i njegove varijante A1 i A2 zauzimaju značajno mesto u istraživanjima različitih autora. Metabolisanjem A1 tipa kod ljudi oslobađa se betakazomorfin-7, koji može da izazove niz oboljenja (dijabetes melitus tip 1, ishemijsku bolest srca, arterosklerozu, sindrom iznenadne smrti odojčadi, autizam, šizofreniju, nelagodnost u gastrointestinalnom sistemu i produženo vreme gastrointestinalne pasaže). Nasuprot tome, metaboliti A2 tipa β-kazeina, usled drugačijeg metabolisanja, ne izazivaju slične efekte po zdravlje ljudi. Cilj ovog istraživanja je bio optimizacija protokola za utvrđivanje polimorfizma β-kazeinskog gena u selekciji krava sa A2A2 genotipom, a prateći principe “jednog zdravlja”. Za analize su korišćeni uzorci krvi iz laboratorijske arhive Katedre za biologiju FVM UB. Amplifikacija i detekcija aminokiselinske razlike između dva tipa β-kazeinskog proteina izvođena je metodom alelski specifične lančane reakcije polimeraze (Allele Specific Polymerase Chain Reaction-AS-PCR), kojoj je prethodila optimizacija temperature hibridizacije prajmera. Vizualizacija rezultata izvedena je pomoću gel-elektroforeze koja je omogućila uočavanje PCR amplikona specifičnih za oba različita alela β-kazeinskog gena, veličine 244 baznih parova. Analizama temperature hibridizacije prajmera, temperatura od 65,50 °C pokazala se kao optimalna. Dobijeni rezultati isptivanja su ukazali na veću frekvencu prisustva alela A2 (0,56) u odnosu na alel A1 (0,44) u ispitivanim uzorcima. Istovremeno od ukupno 35 ispitanih uzoraka kod 7 uzoraka krvi je bilo potvrđeno prisustvo genotipa A1A1 (20,0%), kod 17 uzoraka genotipa A1A2 (48,60%), odnosno kod 11 uzoraka genotipa A2A2 (31,40%). Korišćenjem ove i sličnih metoda u programima uzgoja goveda, mogla bi se izvršiti selekcija goveda koja imaju A2A2 genotip β-kazeina, što bi uslovilo proizvodnju kravljeg mleka bez neželjenog A1 β-kazeinskog proteina i svih njegovih implikacija po zdravlje ljudi.
PB  - Faculty of Veterinary Medicine, University of Belgrade
T2  - Veterinarski glasnik
T1  - Use of allele specific PCR to investigate the presence of β-casein polymorphism in Holstein-Friesian cows
T1  - Upotreba allele specific pcr-a u ispitivanju prisustva polimorfizma β -kazeina kod holštajn-frizijskih krava
VL  - 76
IS  - 1
SP  - 17
EP  - 24
DO  - 10.2298/VETGL211125004R
ER  - 

@article{
author = "Ristanić, Marko and Nikšić, Aleksandar and Niketić, Mia and Jelisić, Stefan and Rajković, Milan and Glavinić, Uroš and Stanimirović, Zoran",
year = "2022",
abstract = "Following the “one health” principle, we have conducted optimization of a protocol for β-casein genotyping in cattle in order to select cows with exclusively the A2A2 genotype. Gastrointestinal proteolysis of A1 β-casein in humans releases beta-casomorphin 7, which is believed to cause a number of diseases/conditions (diabetes mellitus type 1, ischemic heart disease, atherosclerosis, sudden infant death syndrome, autism, schizophrenia, gastrointestinal discomfort, and prolonged gastrointestinal passage time). On the contrary, A2 β-casein does not cause similar effects on human health, due to its different metabolism. DNA extraction was conducted from blood samples belonging to the laboratory archive of the Department of Biology, Faculty of Veterinary Medicine, University of Belgrade. Determination of genotypes was performed using the Allele Specific Polymerase Chain Reaction (AS-PCR) method. The amplification was preceded by determination of proper primer annealing temperature (65.50 °C), in order to ensure optimal genotyping results. The results obtained indicated a higher frequency of the A2 allele (0.56) compared to the A1 allele (0.44). Furthermore, in 7 out of 35 tested samples, the A1A1 genotype (20.00%) was found, in 17 samples, the A1A2 genotype (48.60%) was found, and in 11 samples, the A2A2 genotype (31.40%) was found. The molecular methods used ensured reliable β-casein genotyping that would enable selection of cows with the A2A2 β-casein genotype, implying production of., Kazeini čine 80% ukupnih proteina mleka, dok β-kazein i njegove varijante A1 i A2 zauzimaju značajno mesto u istraživanjima različitih autora. Metabolisanjem A1 tipa kod ljudi oslobađa se betakazomorfin-7, koji može da izazove niz oboljenja (dijabetes melitus tip 1, ishemijsku bolest srca, arterosklerozu, sindrom iznenadne smrti odojčadi, autizam, šizofreniju, nelagodnost u gastrointestinalnom sistemu i produženo vreme gastrointestinalne pasaže). Nasuprot tome, metaboliti A2 tipa β-kazeina, usled drugačijeg metabolisanja, ne izazivaju slične efekte po zdravlje ljudi. Cilj ovog istraživanja je bio optimizacija protokola za utvrđivanje polimorfizma β-kazeinskog gena u selekciji krava sa A2A2 genotipom, a prateći principe “jednog zdravlja”. Za analize su korišćeni uzorci krvi iz laboratorijske arhive Katedre za biologiju FVM UB. Amplifikacija i detekcija aminokiselinske razlike između dva tipa β-kazeinskog proteina izvođena je metodom alelski specifične lančane reakcije polimeraze (Allele Specific Polymerase Chain Reaction-AS-PCR), kojoj je prethodila optimizacija temperature hibridizacije prajmera. Vizualizacija rezultata izvedena je pomoću gel-elektroforeze koja je omogućila uočavanje PCR amplikona specifičnih za oba različita alela β-kazeinskog gena, veličine 244 baznih parova. Analizama temperature hibridizacije prajmera, temperatura od 65,50 °C pokazala se kao optimalna. Dobijeni rezultati isptivanja su ukazali na veću frekvencu prisustva alela A2 (0,56) u odnosu na alel A1 (0,44) u ispitivanim uzorcima. Istovremeno od ukupno 35 ispitanih uzoraka kod 7 uzoraka krvi je bilo potvrđeno prisustvo genotipa A1A1 (20,0%), kod 17 uzoraka genotipa A1A2 (48,60%), odnosno kod 11 uzoraka genotipa A2A2 (31,40%). Korišćenjem ove i sličnih metoda u programima uzgoja goveda, mogla bi se izvršiti selekcija goveda koja imaju A2A2 genotip β-kazeina, što bi uslovilo proizvodnju kravljeg mleka bez neželjenog A1 β-kazeinskog proteina i svih njegovih implikacija po zdravlje ljudi.",
publisher = "Faculty of Veterinary Medicine, University of Belgrade",
journal = "Veterinarski glasnik",
title = "Use of allele specific PCR to investigate the presence of β-casein polymorphism in Holstein-Friesian cows, Upotreba allele specific pcr-a u ispitivanju prisustva polimorfizma β -kazeina kod holštajn-frizijskih krava",
volume = "76",
number = "1",
pages = "17-24",
doi = "10.2298/VETGL211125004R"
}

Ristanić, M., Nikšić, A., Niketić, M., Jelisić, S., Rajković, M., Glavinić, U.,& Stanimirović, Z.. (2022). Use of allele specific PCR to investigate the presence of β-casein polymorphism in Holstein-Friesian cows. in Veterinarski glasnik
Faculty of Veterinary Medicine, University of Belgrade., 76(1), 17-24.
https://doi.org/10.2298/VETGL211125004R

Ristanić M, Nikšić A, Niketić M, Jelisić S, Rajković M, Glavinić U, Stanimirović Z. Use of allele specific PCR to investigate the presence of β-casein polymorphism in Holstein-Friesian cows. in Veterinarski glasnik. 2022;76(1):17-24.
doi:10.2298/VETGL211125004R .

Ristanić, Marko, Nikšić, Aleksandar, Niketić, Mia, Jelisić, Stefan, Rajković, Milan, Glavinić, Uroš, Stanimirović, Zoran, "Use of allele specific PCR to investigate the presence of β-casein polymorphism in Holstein-Friesian cows" in Veterinarski glasnik, 76, no. 1 (2022):17-24,
https://doi.org/10.2298/VETGL211125004R . .

TY  - JOUR
AU  - Rajković, Milan
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Ristanić, Marko
AU  - Vejnović, Branislav
AU  - Goblirsch, Michael
AU  - Glavinić, Uroš
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2502
AB  - Mushroom extracts have been shown to contain bioactive compounds that confer health benefits to humans and animals, including honey bees. Honey bees are often challenged simultaneously with several abiotic (e.g., pesticides and poor nutrition) and biotic (e.g., parasites and pathogens) stressors. Mushroom extracts, for example, those made from Agaricus bisporus, may hold the potential to mitigate the negative effects of these stressors through actions on the honey bee’s immune system, metabolism, and other physiological processes. Exploring the health benefits of mushroom extracts for honey bees requires a basic understanding of their bioactive properties at the cellular level. The aim of the present study was to evaluate, for the first time, the genotoxic and antigenotoxic potential of A. bisporus on the honey bee cell line AmE-711 using the comet assay. Three increasing concentrations of A. bisporus water extract (100, 200, and 400 µg/mL) were tested. The cells in the negative control group were not treated either with A. bisporus extract or with H2O2. In the positive control group, DNA damage was induced with 100 µM H2O2. For antigenotoxic effect, tested extracts were mixed and incubated with H2O2. None of the tested concentrations exerted genotoxic potential, but all showed antigenotoxic effects against H2O2-induced DNA damage. The concentration of 200 μg/mL A. bisporus extract was the most effective in its action against DNA damage. To conclude, A. bisporus extract did not demonstrate genotoxic effects but showed promising antigenotoxic properties. AmE-711 cell line may serve as a cell culture system for genotoxicity investigations.
PB  - Taylor & Francis
T2  - Journal of Apicultural Research
T2  - Journal of Apicultural Research
T1  - Evaluation of genotoxic and genoprotective effects of Agaricus bisporus extract on AmE-711 honey bee cell line in the Comet assay
DO  - 10.1080/00218839.2022.2146367
ER  - 

@article{
author = "Rajković, Milan and Stanimirović, Zoran and Stevanović, Jevrosima and Ristanić, Marko and Vejnović, Branislav and Goblirsch, Michael and Glavinić, Uroš",
year = "2022",
abstract = "Mushroom extracts have been shown to contain bioactive compounds that confer health benefits to humans and animals, including honey bees. Honey bees are often challenged simultaneously with several abiotic (e.g., pesticides and poor nutrition) and biotic (e.g., parasites and pathogens) stressors. Mushroom extracts, for example, those made from Agaricus bisporus, may hold the potential to mitigate the negative effects of these stressors through actions on the honey bee’s immune system, metabolism, and other physiological processes. Exploring the health benefits of mushroom extracts for honey bees requires a basic understanding of their bioactive properties at the cellular level. The aim of the present study was to evaluate, for the first time, the genotoxic and antigenotoxic potential of A. bisporus on the honey bee cell line AmE-711 using the comet assay. Three increasing concentrations of A. bisporus water extract (100, 200, and 400 µg/mL) were tested. The cells in the negative control group were not treated either with A. bisporus extract or with H2O2. In the positive control group, DNA damage was induced with 100 µM H2O2. For antigenotoxic effect, tested extracts were mixed and incubated with H2O2. None of the tested concentrations exerted genotoxic potential, but all showed antigenotoxic effects against H2O2-induced DNA damage. The concentration of 200 μg/mL A. bisporus extract was the most effective in its action against DNA damage. To conclude, A. bisporus extract did not demonstrate genotoxic effects but showed promising antigenotoxic properties. AmE-711 cell line may serve as a cell culture system for genotoxicity investigations.",
publisher = "Taylor & Francis",
journal = "Journal of Apicultural Research, Journal of Apicultural Research",
title = "Evaluation of genotoxic and genoprotective effects of Agaricus bisporus extract on AmE-711 honey bee cell line in the Comet assay",
doi = "10.1080/00218839.2022.2146367"
}

Rajković, M., Stanimirović, Z., Stevanović, J., Ristanić, M., Vejnović, B., Goblirsch, M.,& Glavinić, U.. (2022). Evaluation of genotoxic and genoprotective effects of Agaricus bisporus extract on AmE-711 honey bee cell line in the Comet assay. in Journal of Apicultural Research
Taylor & Francis..
https://doi.org/10.1080/00218839.2022.2146367

Rajković M, Stanimirović Z, Stevanović J, Ristanić M, Vejnović B, Goblirsch M, Glavinić U. Evaluation of genotoxic and genoprotective effects of Agaricus bisporus extract on AmE-711 honey bee cell line in the Comet assay. in Journal of Apicultural Research. 2022;.
doi:10.1080/00218839.2022.2146367 .

Rajković, Milan, Stanimirović, Zoran, Stevanović, Jevrosima, Ristanić, Marko, Vejnović, Branislav, Goblirsch, Michael, Glavinić, Uroš, "Evaluation of genotoxic and genoprotective effects of Agaricus bisporus extract on AmE-711 honey bee cell line in the Comet assay" in Journal of Apicultural Research (2022),
https://doi.org/10.1080/00218839.2022.2146367 . .

TY  - GEN
AU  - Glavinić, Uroš
AU  - Vunduk, Jovana
AU  - Ristanić, Marko
AU  - Rajković, Milan
AU  - Stevanović, Jevrosima
AU  - Vejnović, Branislav
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3039
T2  - Tehničko rešenje kategorije M81 - verifikovano od strane Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije
T1  - Suplement za prihranu pčela na bazi vodenog ekstrakta gljive Agaricus bisporus
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3039
ER  - 

@misc{
author = "Glavinić, Uroš and Vunduk, Jovana and Ristanić, Marko and Rajković, Milan and Stevanović, Jevrosima and Vejnović, Branislav and Stanimirović, Zoran",
year = "2022",
journal = "Tehničko rešenje kategorije M81 - verifikovano od strane Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije",
title = "Suplement za prihranu pčela na bazi vodenog ekstrakta gljive Agaricus bisporus",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3039"
}

Glavinić, U., Vunduk, J., Ristanić, M., Rajković, M., Stevanović, J., Vejnović, B.,& Stanimirović, Z.. (2022). Suplement za prihranu pčela na bazi vodenog ekstrakta gljive Agaricus bisporus. in Tehničko rešenje kategorije M81 - verifikovano od strane Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije.
https://hdl.handle.net/21.15107/rcub_veterinar_3039

Glavinić U, Vunduk J, Ristanić M, Rajković M, Stevanović J, Vejnović B, Stanimirović Z. Suplement za prihranu pčela na bazi vodenog ekstrakta gljive Agaricus bisporus. in Tehničko rešenje kategorije M81 - verifikovano od strane Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije. 2022;.
https://hdl.handle.net/21.15107/rcub_veterinar_3039 .

Glavinić, Uroš, Vunduk, Jovana, Ristanić, Marko, Rajković, Milan, Stevanović, Jevrosima, Vejnović, Branislav, Stanimirović, Zoran, "Suplement za prihranu pčela na bazi vodenog ekstrakta gljive Agaricus bisporus" in Tehničko rešenje kategorije M81 - verifikovano od strane Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije (2022),
https://hdl.handle.net/21.15107/rcub_veterinar_3039 .

TY  - JOUR
AU  - Glavinić, Uroš
AU  - Stevanović, Jevrosima
AU  - Ristanić, Marko
AU  - Rajković, Milan
AU  - Davitkov, Dajana
AU  - Lakić, Nada
AU  - Stanimirović, Zoran
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2047
AB  - Depending on the infection level and colony strength, Nosema ceranae, a microsporidian endoparasite of the honey bee may have significant consequences on the health, reproduction and productivity of bee colonies. Despite exerting some side effects, fumagillin is most often used for Nosema control. In this study, in a cage experiment, N. ceranae infected bees were treated with fumagillin or the extract of Agaricus blazei mushroom, a possible alternative for Nosema control. Bee survival, Nosema spore loads, the expression levels of immune-related genes and parameters of oxidative stress were observed. Fumagillin treatment showed a negative effect on monitored parameters when applied preventively to non-infected bees, while a noticeable anti-Nosema effect and protection from Nosema-induced immunosuppression and oxidative stress were proven in Nosema-infected bees. However, a protective effect of the natural A. blazei extract was detected, without any side effects but with immunostimulatory activity in the preventive application. The results of this research suggest the potential of A. blazei extract for Nosema control, which needs to be further investigated.
PB  - MDPI
T2  - Insects
T1  - Potential of Fumagillin and Agaricus blazei Mushroom Extract to Reduce Nosema ceranae in Honey Bees
VL  - 12
IS  - 4
SP  - 282
DO  - 10.3390/insects12040282
ER  - 

@article{
author = "Glavinić, Uroš and Stevanović, Jevrosima and Ristanić, Marko and Rajković, Milan and Davitkov, Dajana and Lakić, Nada and Stanimirović, Zoran",
year = "2021",
abstract = "Depending on the infection level and colony strength, Nosema ceranae, a microsporidian endoparasite of the honey bee may have significant consequences on the health, reproduction and productivity of bee colonies. Despite exerting some side effects, fumagillin is most often used for Nosema control. In this study, in a cage experiment, N. ceranae infected bees were treated with fumagillin or the extract of Agaricus blazei mushroom, a possible alternative for Nosema control. Bee survival, Nosema spore loads, the expression levels of immune-related genes and parameters of oxidative stress were observed. Fumagillin treatment showed a negative effect on monitored parameters when applied preventively to non-infected bees, while a noticeable anti-Nosema effect and protection from Nosema-induced immunosuppression and oxidative stress were proven in Nosema-infected bees. However, a protective effect of the natural A. blazei extract was detected, without any side effects but with immunostimulatory activity in the preventive application. The results of this research suggest the potential of A. blazei extract for Nosema control, which needs to be further investigated.",
publisher = "MDPI",
journal = "Insects",
title = "Potential of Fumagillin and Agaricus blazei Mushroom Extract to Reduce Nosema ceranae in Honey Bees",
volume = "12",
number = "4",
pages = "282",
doi = "10.3390/insects12040282"
}

Glavinić, U., Stevanović, J., Ristanić, M., Rajković, M., Davitkov, D., Lakić, N.,& Stanimirović, Z.. (2021). Potential of Fumagillin and Agaricus blazei Mushroom Extract to Reduce Nosema ceranae in Honey Bees. in Insects
MDPI., 12(4), 282.
https://doi.org/10.3390/insects12040282

Glavinić U, Stevanović J, Ristanić M, Rajković M, Davitkov D, Lakić N, Stanimirović Z. Potential of Fumagillin and Agaricus blazei Mushroom Extract to Reduce Nosema ceranae in Honey Bees. in Insects. 2021;12(4):282.
doi:10.3390/insects12040282 .

Glavinić, Uroš, Stevanović, Jevrosima, Ristanić, Marko, Rajković, Milan, Davitkov, Dajana, Lakić, Nada, Stanimirović, Zoran, "Potential of Fumagillin and Agaricus blazei Mushroom Extract to Reduce Nosema ceranae in Honey Bees" in Insects, 12, no. 4 (2021):282,
https://doi.org/10.3390/insects12040282 . .

TY  - DATA
AU  - Glavinić, Uroš
AU  - Stevanović, Jevrosima
AU  - Ristanić, Marko
AU  - Rajković, Milan
AU  - Davitkov, Dajana
AU  - Lakić, Nada
AU  - Stanimirović, Zoran
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2048
PB  - MDPI
T2  - Insects
T1  - Supplementary data for article: Glavinic, U.; Stevanovic, J.; Ristanic, M.; Rajkovic, M.; Davitkov, D.; Lakic, N.; Stanimirovic, Z. Potential of Fumagillin and Agaricus Blazei Mushroom Extract to Reduce Nosema Ceranae in Honey Bees. Insects 2021, 12 (4), 282. https://doi.org/10.3390/insects12040282.
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2048
ER  - 

@misc{
author = "Glavinić, Uroš and Stevanović, Jevrosima and Ristanić, Marko and Rajković, Milan and Davitkov, Dajana and Lakić, Nada and Stanimirović, Zoran",
year = "2021",
publisher = "MDPI",
journal = "Insects",
title = "Supplementary data for article: Glavinic, U.; Stevanovic, J.; Ristanic, M.; Rajkovic, M.; Davitkov, D.; Lakic, N.; Stanimirovic, Z. Potential of Fumagillin and Agaricus Blazei Mushroom Extract to Reduce Nosema Ceranae in Honey Bees. Insects 2021, 12 (4), 282. https://doi.org/10.3390/insects12040282.",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2048"
}

Glavinić, U., Stevanović, J., Ristanić, M., Rajković, M., Davitkov, D., Lakić, N.,& Stanimirović, Z.. (2021). Supplementary data for article: Glavinic, U.; Stevanovic, J.; Ristanic, M.; Rajkovic, M.; Davitkov, D.; Lakic, N.; Stanimirovic, Z. Potential of Fumagillin and Agaricus Blazei Mushroom Extract to Reduce Nosema Ceranae in Honey Bees. Insects 2021, 12 (4), 282. https://doi.org/10.3390/insects12040282.. in Insects
MDPI..
https://hdl.handle.net/21.15107/rcub_veterinar_2048

Glavinić U, Stevanović J, Ristanić M, Rajković M, Davitkov D, Lakić N, Stanimirović Z. Supplementary data for article: Glavinic, U.; Stevanovic, J.; Ristanic, M.; Rajkovic, M.; Davitkov, D.; Lakic, N.; Stanimirovic, Z. Potential of Fumagillin and Agaricus Blazei Mushroom Extract to Reduce Nosema Ceranae in Honey Bees. Insects 2021, 12 (4), 282. https://doi.org/10.3390/insects12040282.. in Insects. 2021;.
https://hdl.handle.net/21.15107/rcub_veterinar_2048 .

Glavinić, Uroš, Stevanović, Jevrosima, Ristanić, Marko, Rajković, Milan, Davitkov, Dajana, Lakić, Nada, Stanimirović, Zoran, "Supplementary data for article: Glavinic, U.; Stevanovic, J.; Ristanic, M.; Rajkovic, M.; Davitkov, D.; Lakic, N.; Stanimirovic, Z. Potential of Fumagillin and Agaricus Blazei Mushroom Extract to Reduce Nosema Ceranae in Honey Bees. Insects 2021, 12 (4), 282. https://doi.org/10.3390/insects12040282." in Insects (2021),
https://hdl.handle.net/21.15107/rcub_veterinar_2048 .

TY  - JOUR
AU  - Trailović, Saša M.
AU  - Rajković, Milan
AU  - Marjanović, Đorđe
AU  - Neveu, Cédric
AU  - Charvet, Claude L.
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2232
AB  - Parascaris sp. is the only ascarid parasitic nematode in equids and one of the most threatening infectious organisms in horses. Only a limited number of compounds are available for treatment of horse helminthiasis, and Parascaris sp. worms have developed resistance to the three major anthelmintic families. In order to overcome the appearance of resistance, there is an urgent need for new therapeutic strategies. The active ingredients of herbal essential oils are potentially effective antiparasitic drugs. Carvacrol is one of the principal chemicals of essential oil from Origanum, Thymus, Coridothymus, Thymbra, Satureja and Lippia herbs. However, the antiparasitic mode of action of carvacrol is poorly understood. Here, the objective of the work was to characterize the activity of carvacrol on Parascaris sp. nicotinic acetylcholine receptor (nAChR) function both in vivo with the use of worm neuromuscular flap preparations and in vitro with two-electrode voltage-clamp electrophysiology on nAChRs expressed in Xenopus oocytes. We developed a neuromuscular contraction assay for Parascaris body flaps and obtained acetylcholine concentration-dependent contraction responses. Strikingly, we observed that 300 µM carvacrol fully and irreversibly abolished Parascaris sp. muscle contractions elicited by acetylcholine. Similarly, carvacrol antagonized acetylcholine-induced currents from both the nicotine-sensitive AChR and the morantel-sensitive AChR subtypes. Thus, we show for the first time that body muscle flap preparation is a tractable approach to investigating the pharmacology of Parascaris sp. neuromuscular system. Our results suggest an intriguing mode of action for carvacrol, being a potent antagonist of muscle nAChRs of Parascaris sp. worms, which may account for its antiparasitic potency.
T2  - Pharmaceuticals
T1  - Action of Carvacrol on Parascaris sp. and Antagonistic Effect on Nicotinic Acetylcholine Receptors
VL  - 14
IS  - 6
SP  - 505
DO  - 10.3390/ph14060505
ER  - 

@article{
author = "Trailović, Saša M. and Rajković, Milan and Marjanović, Đorđe and Neveu, Cédric and Charvet, Claude L.",
year = "2021",
abstract = "Parascaris sp. is the only ascarid parasitic nematode in equids and one of the most threatening infectious organisms in horses. Only a limited number of compounds are available for treatment of horse helminthiasis, and Parascaris sp. worms have developed resistance to the three major anthelmintic families. In order to overcome the appearance of resistance, there is an urgent need for new therapeutic strategies. The active ingredients of herbal essential oils are potentially effective antiparasitic drugs. Carvacrol is one of the principal chemicals of essential oil from Origanum, Thymus, Coridothymus, Thymbra, Satureja and Lippia herbs. However, the antiparasitic mode of action of carvacrol is poorly understood. Here, the objective of the work was to characterize the activity of carvacrol on Parascaris sp. nicotinic acetylcholine receptor (nAChR) function both in vivo with the use of worm neuromuscular flap preparations and in vitro with two-electrode voltage-clamp electrophysiology on nAChRs expressed in Xenopus oocytes. We developed a neuromuscular contraction assay for Parascaris body flaps and obtained acetylcholine concentration-dependent contraction responses. Strikingly, we observed that 300 µM carvacrol fully and irreversibly abolished Parascaris sp. muscle contractions elicited by acetylcholine. Similarly, carvacrol antagonized acetylcholine-induced currents from both the nicotine-sensitive AChR and the morantel-sensitive AChR subtypes. Thus, we show for the first time that body muscle flap preparation is a tractable approach to investigating the pharmacology of Parascaris sp. neuromuscular system. Our results suggest an intriguing mode of action for carvacrol, being a potent antagonist of muscle nAChRs of Parascaris sp. worms, which may account for its antiparasitic potency.",
journal = "Pharmaceuticals",
title = "Action of Carvacrol on Parascaris sp. and Antagonistic Effect on Nicotinic Acetylcholine Receptors",
volume = "14",
number = "6",
pages = "505",
doi = "10.3390/ph14060505"
}

Trailović, S. M., Rajković, M., Marjanović, Đ., Neveu, C.,& Charvet, C. L.. (2021). Action of Carvacrol on Parascaris sp. and Antagonistic Effect on Nicotinic Acetylcholine Receptors. in Pharmaceuticals, 14(6), 505.
https://doi.org/10.3390/ph14060505

Trailović SM, Rajković M, Marjanović Đ, Neveu C, Charvet CL. Action of Carvacrol on Parascaris sp. and Antagonistic Effect on Nicotinic Acetylcholine Receptors. in Pharmaceuticals. 2021;14(6):505.
doi:10.3390/ph14060505 .

Trailović, Saša M., Rajković, Milan, Marjanović, Đorđe, Neveu, Cédric, Charvet, Claude L., "Action of Carvacrol on Parascaris sp. and Antagonistic Effect on Nicotinic Acetylcholine Receptors" in Pharmaceuticals, 14, no. 6 (2021):505,
https://doi.org/10.3390/ph14060505 . .

TY  - JOUR
AU  - Glavinić, Uroš
AU  - Rajković, Milan
AU  - Vunduk, Jovana
AU  - Vejnović, Branislav
AU  - Stevanović, Jevrosima
AU  - Milenković, Ivanka
AU  - Stanimirović, Zoran
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2234
AB  - Agaricus bisporus water crude extract was tested on honey bees for the first time. The first part of the cage experiment was set for selecting one concentration of the A. bisporus extract. Concentration of 200 µg/g was further tested in the second part of the experiment where bee survival and food consumption were monitored together with Nosema infection level and expression of five genes (abaecin, hymenoptaecin, defensin, apidaecin, and vitellogenin) that were evaluated in bees sampled on days 7 and 15. Survival rate of Nosema-infected bees was significantly greater in groups fed with A. bisporus-enriched syrup compared to those fed with a pure sucrose syrup. Besides, the anti-Nosema effect of A. bisporus extract was greatest when applied from the third day which coincides with the time of infection with N. ceranae. Daily food consumption did not differ between the groups indicating good acceptability and palatability of the extract. A. bisporus extract showed a stimulative effect on four out of five monitored genes. Both anti-Nosema and nutrigenomic effects of A. bisporus extract were observed when supplementation started at the moment of N. ceranae infection or preventively (before or simultaneously with the infection).
PB  - MDPI
T2  - Insects
T1  - Effects of Agaricus bisporus Mushroom Extract on Honey Bees Infected with Nosema ceranae
VL  - 12
IS  - 10
SP  - 915
DO  - 10.3390/insects12100915
ER  - 

@article{
author = "Glavinić, Uroš and Rajković, Milan and Vunduk, Jovana and Vejnović, Branislav and Stevanović, Jevrosima and Milenković, Ivanka and Stanimirović, Zoran",
year = "2021",
abstract = "Agaricus bisporus water crude extract was tested on honey bees for the first time. The first part of the cage experiment was set for selecting one concentration of the A. bisporus extract. Concentration of 200 µg/g was further tested in the second part of the experiment where bee survival and food consumption were monitored together with Nosema infection level and expression of five genes (abaecin, hymenoptaecin, defensin, apidaecin, and vitellogenin) that were evaluated in bees sampled on days 7 and 15. Survival rate of Nosema-infected bees was significantly greater in groups fed with A. bisporus-enriched syrup compared to those fed with a pure sucrose syrup. Besides, the anti-Nosema effect of A. bisporus extract was greatest when applied from the third day which coincides with the time of infection with N. ceranae. Daily food consumption did not differ between the groups indicating good acceptability and palatability of the extract. A. bisporus extract showed a stimulative effect on four out of five monitored genes. Both anti-Nosema and nutrigenomic effects of A. bisporus extract were observed when supplementation started at the moment of N. ceranae infection or preventively (before or simultaneously with the infection).",
publisher = "MDPI",
journal = "Insects",
title = "Effects of Agaricus bisporus Mushroom Extract on Honey Bees Infected with Nosema ceranae",
volume = "12",
number = "10",
pages = "915",
doi = "10.3390/insects12100915"
}

Glavinić, U., Rajković, M., Vunduk, J., Vejnović, B., Stevanović, J., Milenković, I.,& Stanimirović, Z.. (2021). Effects of Agaricus bisporus Mushroom Extract on Honey Bees Infected with Nosema ceranae. in Insects
MDPI., 12(10), 915.
https://doi.org/10.3390/insects12100915

Glavinić U, Rajković M, Vunduk J, Vejnović B, Stevanović J, Milenković I, Stanimirović Z. Effects of Agaricus bisporus Mushroom Extract on Honey Bees Infected with Nosema ceranae. in Insects. 2021;12(10):915.
doi:10.3390/insects12100915 .

Glavinić, Uroš, Rajković, Milan, Vunduk, Jovana, Vejnović, Branislav, Stevanović, Jevrosima, Milenković, Ivanka, Stanimirović, Zoran, "Effects of Agaricus bisporus Mushroom Extract on Honey Bees Infected with Nosema ceranae" in Insects, 12, no. 10 (2021):915,
https://doi.org/10.3390/insects12100915 . .

TY  - DATA
AU  - Glavinić, Uroš
AU  - Rajković, Milan
AU  - Vunduk, Jovana
AU  - Vejnović, Branislav
AU  - Stevanović, Jevrosima
AU  - Milenković, Ivanka
AU  - Stanimirović, Zoran
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2235
PB  - MDPI
T2  - Insects
T1  - Supplementary data for article: Glavinić, U.; Rajković, M.; Vunduk, J.; Vejnović, B.; Stevanović, J.; Milenković, I.; Stanimirović, Z. Effects of Agaricus bisporus Mushroom Extract on Honey Bees Infected with Nosema ceranae. Insects 2021, 12 (10). https://doi.org/10.3390/insects12100915.
VL  - 12
IS  - 10
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2235
ER  - 

@misc{
author = "Glavinić, Uroš and Rajković, Milan and Vunduk, Jovana and Vejnović, Branislav and Stevanović, Jevrosima and Milenković, Ivanka and Stanimirović, Zoran",
year = "2021",
publisher = "MDPI",
journal = "Insects",
title = "Supplementary data for article: Glavinić, U.; Rajković, M.; Vunduk, J.; Vejnović, B.; Stevanović, J.; Milenković, I.; Stanimirović, Z. Effects of Agaricus bisporus Mushroom Extract on Honey Bees Infected with Nosema ceranae. Insects 2021, 12 (10). https://doi.org/10.3390/insects12100915.",
volume = "12",
number = "10",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2235"
}

Glavinić, U., Rajković, M., Vunduk, J., Vejnović, B., Stevanović, J., Milenković, I.,& Stanimirović, Z.. (2021). Supplementary data for article: Glavinić, U.; Rajković, M.; Vunduk, J.; Vejnović, B.; Stevanović, J.; Milenković, I.; Stanimirović, Z. Effects of Agaricus bisporus Mushroom Extract on Honey Bees Infected with Nosema ceranae. Insects 2021, 12 (10). https://doi.org/10.3390/insects12100915.. in Insects
MDPI., 12(10).
https://hdl.handle.net/21.15107/rcub_veterinar_2235

Glavinić U, Rajković M, Vunduk J, Vejnović B, Stevanović J, Milenković I, Stanimirović Z. Supplementary data for article: Glavinić, U.; Rajković, M.; Vunduk, J.; Vejnović, B.; Stevanović, J.; Milenković, I.; Stanimirović, Z. Effects of Agaricus bisporus Mushroom Extract on Honey Bees Infected with Nosema ceranae. Insects 2021, 12 (10). https://doi.org/10.3390/insects12100915.. in Insects. 2021;12(10).
https://hdl.handle.net/21.15107/rcub_veterinar_2235 .

Glavinić, Uroš, Rajković, Milan, Vunduk, Jovana, Vejnović, Branislav, Stevanović, Jevrosima, Milenković, Ivanka, Stanimirović, Zoran, "Supplementary data for article: Glavinić, U.; Rajković, M.; Vunduk, J.; Vejnović, B.; Stevanović, J.; Milenković, I.; Stanimirović, Z. Effects of Agaricus bisporus Mushroom Extract on Honey Bees Infected with Nosema ceranae. Insects 2021, 12 (10). https://doi.org/10.3390/insects12100915." in Insects, 12, no. 10 (2021),
https://hdl.handle.net/21.15107/rcub_veterinar_2235 .

TY  - JOUR
AU  - Rajković, Milan
AU  - Glavinić, Uroš
AU  - Ristanić, Marko
AU  - Ćosić, Milivoje
AU  - Dimitrijević-Srećković, Vesna
AU  - Ilić, Iva
AU  - Đelić, Ninoslav
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2239
AB  - Diabetes is one of the main health concerns, especially in developed countries. During the last few decades, the percentage of diabetic persons is constantly increasing. Although the genetic factors have a strong influence in the development of diabetes, environmental influence (physical inactivity, inadequate nutrition leading to obesity) also have an important impact. The main objective of this investigation was to evaluate the possible influence of organic sprouted whole wheat grain flourless bread Tonus® (product of Trivit, Becej, Serbia) on DNA damage at various stages of progression through type 2 diabetes mellitus (T2DM). In addition to control (non-diabetic) subjects we analyzed obese, pre-diabetic and diabetic patients, for a total of four experimental groups. All subjects used to eat Mediterranean diet for at least two years before being included in our study. In each of four groups we had five persons practicing the Mediterranean diet but instead of bread they consumed flourless bread Tonus®. The DNA damage was evaluated on peripheral blood mononuclear cells by alkaline single cell gel electrophoresis (Comet) assay at the very beginning (before starting the Tonus® bread diet), and after exactly the three months of consumption of Tonus® bread. Statistical analysis revealed that only in patients with type
AB  - Dijabetes predstavlja jedan od glavnih zdravstvenih problema, naročito u razvijenim zemljama. Tokom poslednjih nekoliko decenija neprekidno se povećava procenat osoba obolelih od dijabetesa. Mada genetički faktori imaju snažan uticaj na razvoj dijabetesa, uticaji sredine (fizička neaktivnost, nepravilna ishrana koja dovodi do gojaznosti) takođe imaju veliki uticaj na razvoj ove bolesti. Tokom nekoliko poslednjih decenija procenat osoba sa dijabetesom se konstantno povećava. Glavni cilj ovog istraživanja bio je da se proceni mogući uticaj organskog hleba bez brašna od celih proklijalih zrna pšenice, Tonus® (Trivit, Bečej, Serbia) na oštećenja DNK u različitim stadijumima progresije kroz tip 2 dijabetes melitus. Pored kontrolne grupe (zdrave osobe), analizirali smo gojazne, predijabetičare i dijabetičare, što čini ukupno četiri eksperimentalne grupe. Sve osobe su koristile Mediteransku ishranu najmanje dve godine pre nego su uključene u ovu studiju. U svakoj grupi imali smo pet osoba koje su upražnjavale Mediteransku ishranu, ali su umesto uobičajenog hleba konzumirali hleb bez brašna Tonus®. Oštećenja DNK su evaluirana alkalnom verzijom elektoroforeze DNK pojedinačnih ćelija (komet test) na samom početku (pre uvođenja hleba Tonus® u ishranu) i tačno nakon tri meseca konzumiranja hleba Tonus®. Statistička analiza otkrila je da samo kod pacijenata sa dijabetesom tipa 2 konzumiranje Tonus hleba dovodi do smanjenja oštećenja DNK u poređenju sa stepenom oštećenja DNK kod ovih pacijenata pre nego su otpočeli dijetu sa hlebom Tonus. Pretpostaljamo da je pad hiperinsulinemije usled konzumiranja hleba Tonus mogao da bude jedan od glavnih uzroka smanjenja oštećenja DNK.
PB  - Faculty of Veterinary Medicine, Belgrade University
T2  - Acta Veterinaria
T2  - Acta Veterinaria-Beograd
T1  - Does organic sprouted whole wheat grain flourless bread decreases DNA damage in diabetic patients?
T1  - Da li organski hleb bez brašna od celih proklijalih zrna pšenice smanjuje oštećenja DNK kod pacijenata sa dijabetesom?
VL  - 71
IS  - 3
SP  - 273
EP  - 284
DO  - 10.2478/acve-2021-0024
ER  - 

@article{
author = "Rajković, Milan and Glavinić, Uroš and Ristanić, Marko and Ćosić, Milivoje and Dimitrijević-Srećković, Vesna and Ilić, Iva and Đelić, Ninoslav",
year = "2021",
abstract = "Diabetes is one of the main health concerns, especially in developed countries. During the last few decades, the percentage of diabetic persons is constantly increasing. Although the genetic factors have a strong influence in the development of diabetes, environmental influence (physical inactivity, inadequate nutrition leading to obesity) also have an important impact. The main objective of this investigation was to evaluate the possible influence of organic sprouted whole wheat grain flourless bread Tonus® (product of Trivit, Becej, Serbia) on DNA damage at various stages of progression through type 2 diabetes mellitus (T2DM). In addition to control (non-diabetic) subjects we analyzed obese, pre-diabetic and diabetic patients, for a total of four experimental groups. All subjects used to eat Mediterranean diet for at least two years before being included in our study. In each of four groups we had five persons practicing the Mediterranean diet but instead of bread they consumed flourless bread Tonus®. The DNA damage was evaluated on peripheral blood mononuclear cells by alkaline single cell gel electrophoresis (Comet) assay at the very beginning (before starting the Tonus® bread diet), and after exactly the three months of consumption of Tonus® bread. Statistical analysis revealed that only in patients with type, Dijabetes predstavlja jedan od glavnih zdravstvenih problema, naročito u razvijenim zemljama. Tokom poslednjih nekoliko decenija neprekidno se povećava procenat osoba obolelih od dijabetesa. Mada genetički faktori imaju snažan uticaj na razvoj dijabetesa, uticaji sredine (fizička neaktivnost, nepravilna ishrana koja dovodi do gojaznosti) takođe imaju veliki uticaj na razvoj ove bolesti. Tokom nekoliko poslednjih decenija procenat osoba sa dijabetesom se konstantno povećava. Glavni cilj ovog istraživanja bio je da se proceni mogući uticaj organskog hleba bez brašna od celih proklijalih zrna pšenice, Tonus® (Trivit, Bečej, Serbia) na oštećenja DNK u različitim stadijumima progresije kroz tip 2 dijabetes melitus. Pored kontrolne grupe (zdrave osobe), analizirali smo gojazne, predijabetičare i dijabetičare, što čini ukupno četiri eksperimentalne grupe. Sve osobe su koristile Mediteransku ishranu najmanje dve godine pre nego su uključene u ovu studiju. U svakoj grupi imali smo pet osoba koje su upražnjavale Mediteransku ishranu, ali su umesto uobičajenog hleba konzumirali hleb bez brašna Tonus®. Oštećenja DNK su evaluirana alkalnom verzijom elektoroforeze DNK pojedinačnih ćelija (komet test) na samom početku (pre uvođenja hleba Tonus® u ishranu) i tačno nakon tri meseca konzumiranja hleba Tonus®. Statistička analiza otkrila je da samo kod pacijenata sa dijabetesom tipa 2 konzumiranje Tonus hleba dovodi do smanjenja oštećenja DNK u poređenju sa stepenom oštećenja DNK kod ovih pacijenata pre nego su otpočeli dijetu sa hlebom Tonus. Pretpostaljamo da je pad hiperinsulinemije usled konzumiranja hleba Tonus mogao da bude jedan od glavnih uzroka smanjenja oštećenja DNK.",
publisher = "Faculty of Veterinary Medicine, Belgrade University",
journal = "Acta Veterinaria, Acta Veterinaria-Beograd",
title = "Does organic sprouted whole wheat grain flourless bread decreases DNA damage in diabetic patients?, Da li organski hleb bez brašna od celih proklijalih zrna pšenice smanjuje oštećenja DNK kod pacijenata sa dijabetesom?",
volume = "71",
number = "3",
pages = "273-284",
doi = "10.2478/acve-2021-0024"
}

Rajković, M., Glavinić, U., Ristanić, M., Ćosić, M., Dimitrijević-Srećković, V., Ilić, I.,& Đelić, N.. (2021). Does organic sprouted whole wheat grain flourless bread decreases DNA damage in diabetic patients?. in Acta Veterinaria
Faculty of Veterinary Medicine, Belgrade University., 71(3), 273-284.
https://doi.org/10.2478/acve-2021-0024

Rajković M, Glavinić U, Ristanić M, Ćosić M, Dimitrijević-Srećković V, Ilić I, Đelić N. Does organic sprouted whole wheat grain flourless bread decreases DNA damage in diabetic patients?. in Acta Veterinaria. 2021;71(3):273-284.
doi:10.2478/acve-2021-0024 .

Rajković, Milan, Glavinić, Uroš, Ristanić, Marko, Ćosić, Milivoje, Dimitrijević-Srećković, Vesna, Ilić, Iva, Đelić, Ninoslav, "Does organic sprouted whole wheat grain flourless bread decreases DNA damage in diabetic patients?" in Acta Veterinaria, 71, no. 3 (2021):273-284,
https://doi.org/10.2478/acve-2021-0024 . .

TY  - CONF
AU  - Marjanović, Đorđe S.
AU  - Rajković, Milan
AU  - Neveu, Cédric
AU  - Trailović, Saša
AU  - Charvet, Claude
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3417
AB  - Parascaris sp. is the only ascarid parasitic nematode in equids and one of the most threatening infectious organisms in horses. Only a limited number of compounds are available for treatment of horse helminthiasis and Parascaris sp. worms have developed resistance to the three major anthelmintic families. In order to overcome the appearance of resistance, there is an urgent need for new therapeutic strategies. The active ingredients of herbal essential oils are potentially effective antiparasitic drugs. Carvacrol is one of the principal chemical of essential oil from Origanum, Thymus, Coridothymus, Thymbra, Satureja and Lippia herbs. However the antiparasitic mode of action of carvacrol is poorly understood so far. Here, the objective of the work was to characterize and investigate the activity of carvacrol on Parascaris sp. nicotinic acetylcholine receptors (AChRs) function both in vivo with the use of worm neuro-muscular flap preparations and in vitro with elecrophysiology on nAChRs expressed in Xenopus oocytes. Strikingly, we observed that carvacrol fully abolished Parascaris sp. muscle contractions elicited by acetylcholine. Conversely, carvacrol inhibited acetylcholine-induced currents on both the nicotine-sentitive AChR and the morantel-sensitive AChR subtypes. Thus, we show for the first time that the body muscle flap preparation is a tractable approach to investigate the pharmacology of Parascaris sp. neuro-muscular system. Our results suggest an intriguing mode of action for carvacrol being a potent antagonist of muscle nAChRs of Parascaris sp. worms which may account for its antiparasitic potency.
C3  - 28-th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP 2021), Dublin, 2021
T1  - Antagonistic effect of carvacrol on nicotinic acetylcholine receptors from the horse parasite Parascaris sp.
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3417
ER  - 

@conference{
author = "Marjanović, Đorđe S. and Rajković, Milan and Neveu, Cédric and Trailović, Saša and Charvet, Claude",
year = "2021",
abstract = "Parascaris sp. is the only ascarid parasitic nematode in equids and one of the most threatening infectious organisms in horses. Only a limited number of compounds are available for treatment of horse helminthiasis and Parascaris sp. worms have developed resistance to the three major anthelmintic families. In order to overcome the appearance of resistance, there is an urgent need for new therapeutic strategies. The active ingredients of herbal essential oils are potentially effective antiparasitic drugs. Carvacrol is one of the principal chemical of essential oil from Origanum, Thymus, Coridothymus, Thymbra, Satureja and Lippia herbs. However the antiparasitic mode of action of carvacrol is poorly understood so far. Here, the objective of the work was to characterize and investigate the activity of carvacrol on Parascaris sp. nicotinic acetylcholine receptors (AChRs) function both in vivo with the use of worm neuro-muscular flap preparations and in vitro with elecrophysiology on nAChRs expressed in Xenopus oocytes. Strikingly, we observed that carvacrol fully abolished Parascaris sp. muscle contractions elicited by acetylcholine. Conversely, carvacrol inhibited acetylcholine-induced currents on both the nicotine-sentitive AChR and the morantel-sensitive AChR subtypes. Thus, we show for the first time that the body muscle flap preparation is a tractable approach to investigate the pharmacology of Parascaris sp. neuro-muscular system. Our results suggest an intriguing mode of action for carvacrol being a potent antagonist of muscle nAChRs of Parascaris sp. worms which may account for its antiparasitic potency.",
journal = "28-th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP 2021), Dublin, 2021",
title = "Antagonistic effect of carvacrol on nicotinic acetylcholine receptors from the horse parasite Parascaris sp.",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3417"
}

Marjanović, Đ. S., Rajković, M., Neveu, C., Trailović, S.,& Charvet, C.. (2021). Antagonistic effect of carvacrol on nicotinic acetylcholine receptors from the horse parasite Parascaris sp.. in 28-th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP 2021), Dublin, 2021.
https://hdl.handle.net/21.15107/rcub_veterinar_3417

Marjanović ĐS, Rajković M, Neveu C, Trailović S, Charvet C. Antagonistic effect of carvacrol on nicotinic acetylcholine receptors from the horse parasite Parascaris sp.. in 28-th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP 2021), Dublin, 2021. 2021;.
https://hdl.handle.net/21.15107/rcub_veterinar_3417 .

Marjanović, Đorđe S., Rajković, Milan, Neveu, Cédric, Trailović, Saša, Charvet, Claude, "Antagonistic effect of carvacrol on nicotinic acetylcholine receptors from the horse parasite Parascaris sp." in 28-th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP 2021), Dublin, 2021 (2021),
https://hdl.handle.net/21.15107/rcub_veterinar_3417 .

TY  - JOUR
AU  - Rajković, Milan
AU  - Vučićević, Ivana
AU  - Vučićević, Miloš
AU  - Dosenović, Milan
AU  - Charvet, L. Claude
AU  - Resanović, Radmila
AU  - Trailović, Saša
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1696
AB  - We present a case of Ascaridia galli infection in laying hens on a farm in central Serbia. During the rearing period on litter, ascaridosis was diagnosed at 15 weeks of age by routine parasitological fecal examination. Pullets were treated with flubendazole for one week, and two weeks later the hens were moved to battery cages. The production results were within technological standards until the 23rd week and the medical health status was without any clinical symptoms. After that period weight loss began, the egg production dropped to 70% and eggs were of poor quality. Subsequently, severe feather pecking and an increase of mortality were reported. The postmortal examination showed severe anemia and several gross lesions in the liver, intestines, lungs, and kidneys. Different sizes of A. galli were found in the lumen of the duodenum and jejunum. Gross changes of the intestinal mucosa were present, such as a thickened intestinal wall with hemorrhagic spots, inflammation and necrotic patches. Histopathological examination showed marked changes in the intestines, liver and kidneys. All visible live parasites were collected and stored in Earles balanced salts, and females were used for in vitro susceptibility testing. Median lethal concentration (LC50) of piperazine, levamisole and carvacrol for A. galli was 119.7 mu M, 2.71 mu M and 3.26 mu M, were applied, respectively. Based on our results, it is likely that reinfection occurred after completed dehelmintization. In relation to the new circumstances and the regulation for laying hen welfare the deworming protocol should be changed in order to ensure successful dehelmintization. In order to prevent reinfection the treatment must be done at the end of the rearing period and thus be maximally effective.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Ascaridia galli infection in laying hens and the results of in vitro efficacy of levamisole, piperazine and carvacrol, whether is necessary to change the deworming protocols?
VL  - 69
IS  - 4
SP  - 414
EP  - 425
DO  - 10.2478/acve-2019-0035
ER  - 

@article{
author = "Rajković, Milan and Vučićević, Ivana and Vučićević, Miloš and Dosenović, Milan and Charvet, L. Claude and Resanović, Radmila and Trailović, Saša",
year = "2019",
abstract = "We present a case of Ascaridia galli infection in laying hens on a farm in central Serbia. During the rearing period on litter, ascaridosis was diagnosed at 15 weeks of age by routine parasitological fecal examination. Pullets were treated with flubendazole for one week, and two weeks later the hens were moved to battery cages. The production results were within technological standards until the 23rd week and the medical health status was without any clinical symptoms. After that period weight loss began, the egg production dropped to 70% and eggs were of poor quality. Subsequently, severe feather pecking and an increase of mortality were reported. The postmortal examination showed severe anemia and several gross lesions in the liver, intestines, lungs, and kidneys. Different sizes of A. galli were found in the lumen of the duodenum and jejunum. Gross changes of the intestinal mucosa were present, such as a thickened intestinal wall with hemorrhagic spots, inflammation and necrotic patches. Histopathological examination showed marked changes in the intestines, liver and kidneys. All visible live parasites were collected and stored in Earles balanced salts, and females were used for in vitro susceptibility testing. Median lethal concentration (LC50) of piperazine, levamisole and carvacrol for A. galli was 119.7 mu M, 2.71 mu M and 3.26 mu M, were applied, respectively. Based on our results, it is likely that reinfection occurred after completed dehelmintization. In relation to the new circumstances and the regulation for laying hen welfare the deworming protocol should be changed in order to ensure successful dehelmintization. In order to prevent reinfection the treatment must be done at the end of the rearing period and thus be maximally effective.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Ascaridia galli infection in laying hens and the results of in vitro efficacy of levamisole, piperazine and carvacrol, whether is necessary to change the deworming protocols?",
volume = "69",
number = "4",
pages = "414-425",
doi = "10.2478/acve-2019-0035"
}

Rajković, M., Vučićević, I., Vučićević, M., Dosenović, M., Charvet, L. C., Resanović, R.,& Trailović, S.. (2019). Ascaridia galli infection in laying hens and the results of in vitro efficacy of levamisole, piperazine and carvacrol, whether is necessary to change the deworming protocols?. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(4), 414-425.
https://doi.org/10.2478/acve-2019-0035

Rajković M, Vučićević I, Vučićević M, Dosenović M, Charvet LC, Resanović R, Trailović S. Ascaridia galli infection in laying hens and the results of in vitro efficacy of levamisole, piperazine and carvacrol, whether is necessary to change the deworming protocols?. in Acta Veterinaria-Beograd. 2019;69(4):414-425.
doi:10.2478/acve-2019-0035 .

Rajković, Milan, Vučićević, Ivana, Vučićević, Miloš, Dosenović, Milan, Charvet, L. Claude, Resanović, Radmila, Trailović, Saša, "Ascaridia galli infection in laying hens and the results of in vitro efficacy of levamisole, piperazine and carvacrol, whether is necessary to change the deworming protocols?" in Acta Veterinaria-Beograd, 69, no. 4 (2019):414-425,
https://doi.org/10.2478/acve-2019-0035 . .

TY  - CONF
AU  - Stevanović, Jevrosima
AU  - Stanišić, Ljubodrag
AU  - Rajković, Milan
AU  - Ristanić, Marko
AU  - Dimitrijević, Vladimir
AU  - Stojanović, Srđan
AU  - Stanimirović, Zoran
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2864
AB  - Molekularno-genetička karakterizacija autohtonih rasa ţivotinja je značajna jer
doprinosi razvoju efektivnih planova za odrţivo korišćenje i konzervaciju animalnih genetskih resursa (AnGR). U ovom radu prikazani su najvaţniji rezultati
molekularno-genetičkih istraţivanja koje su autori ostvarili u okrivu projekata
III460021
i D310281 analizom autohtonih rasa magaraca, goveda, pasa, pčela i
njihovih parazita sa teritorije Srbije, Revijalno su predstavljeni i najznačajniji
rezultati drugih istraţivanja, ali samo kada je reč o ovcama
AB  - Molecular genetic characterization of indigenous animal breeds is important as
it contributes to the development of effective plans for sustainable use and
conservation of AnGR. This paper presents the most important results of
molecular genetic investigation the authors performed within the projects
III46002 and i D31028 by the analysis of indigenous breeds of donkeys, cattle,
dogs, bees and their parasites from the territory of Serbia. The most significant
results of other studies were presented in the case of sheep.
PB  - Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu
C3  - Drugi simpozijum Zaštita agrobiodiverziterta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 28 - 30. jun, 2019
T1  - Molekularno-genetička karakterizacija nekih autohtonih rasa životinja u Srbiji
SP  - 10
EP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2864
ER  - 

@conference{
author = "Stevanović, Jevrosima and Stanišić, Ljubodrag and Rajković, Milan and Ristanić, Marko and Dimitrijević, Vladimir and Stojanović, Srđan and Stanimirović, Zoran",
year = "2019",
abstract = "Molekularno-genetička karakterizacija autohtonih rasa ţivotinja je značajna jer
doprinosi razvoju efektivnih planova za odrţivo korišćenje i konzervaciju animalnih genetskih resursa (AnGR). U ovom radu prikazani su najvaţniji rezultati
molekularno-genetičkih istraţivanja koje su autori ostvarili u okrivu projekata
III460021
i D310281 analizom autohtonih rasa magaraca, goveda, pasa, pčela i
njihovih parazita sa teritorije Srbije, Revijalno su predstavljeni i najznačajniji
rezultati drugih istraţivanja, ali samo kada je reč o ovcama, Molecular genetic characterization of indigenous animal breeds is important as
it contributes to the development of effective plans for sustainable use and
conservation of AnGR. This paper presents the most important results of
molecular genetic investigation the authors performed within the projects
III46002 and i D31028 by the analysis of indigenous breeds of donkeys, cattle,
dogs, bees and their parasites from the territory of Serbia. The most significant
results of other studies were presented in the case of sheep.",
publisher = "Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu",
journal = "Drugi simpozijum Zaštita agrobiodiverziterta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 28 - 30. jun, 2019",
title = "Molekularno-genetička karakterizacija nekih autohtonih rasa životinja u Srbiji",
pages = "10-18",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2864"
}

Stevanović, J., Stanišić, L., Rajković, M., Ristanić, M., Dimitrijević, V., Stojanović, S.,& Stanimirović, Z.. (2019). Molekularno-genetička karakterizacija nekih autohtonih rasa životinja u Srbiji. in Drugi simpozijum Zaštita agrobiodiverziterta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 28 - 30. jun, 2019
Beograd : Fakultet veterinarske medicine, Univerzitet u Beogradu., 10-18.
https://hdl.handle.net/21.15107/rcub_veterinar_2864

Stevanović J, Stanišić L, Rajković M, Ristanić M, Dimitrijević V, Stojanović S, Stanimirović Z. Molekularno-genetička karakterizacija nekih autohtonih rasa životinja u Srbiji. in Drugi simpozijum Zaštita agrobiodiverziterta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 28 - 30. jun, 2019. 2019;:10-18.
https://hdl.handle.net/21.15107/rcub_veterinar_2864 .

Stevanović, Jevrosima, Stanišić, Ljubodrag, Rajković, Milan, Ristanić, Marko, Dimitrijević, Vladimir, Stojanović, Srđan, Stanimirović, Zoran, "Molekularno-genetička karakterizacija nekih autohtonih rasa životinja u Srbiji" in Drugi simpozijum Zaštita agrobiodiverziterta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 28 - 30. jun, 2019 (2019):10-18,
https://hdl.handle.net/21.15107/rcub_veterinar_2864 .