TY  - CONF
AU  - Bugarski, Dejan
AU  - Petrović, Tamaš
AU  - Lupulović, Diana
AU  - Lazić, Sava
AU  - Todorović, Dalibor
AU  - Savić, Sara
AU  - Bojkovski, Jovan
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3520
AB  - Инфекције дисајних органа говеда са својом мултифакторијалном етиологијом су сталан
проблем говедарске производње и истовремено изазов за ветеринарску струку. Најугроженије
узрасне и производне групе говеда, на фармама крава су телад старости до 4 месеца, а у
товилиштима телад на почетку това, најчешће у тежини између 100 и 250kg. На морбидитет, а
касније и на морталитет утичу услови држања, поступак са животињама, имуни статус,
изложеност микроорганизмима, врста присутних патогених микроорганизама.
Мултифакторијалност узрока овог обољења отежава постизање потпуне профилаксе те се пре у
овом случају може говорити о предузимању мера за смањење ризика и свођење трошкова које
узрокује обољење у прихватљив оквир. Штете које инфекције дисајних органа говеда наносе су
најизраженије у тову говеда. Са ветеринарског становишта, спровођење имунопрофилаксе се
сматра најделотворнијом и најлакшом методом у сузбијању респираторних инфекција говеда.
Међутим, у пракси се она не ретко показује као непотпуно успешна што доводи до незадовољства
власника животиња и његовог удаљавања од ветеринара. Узроци неуспеха спроведене
имунопрофилаксе су неподударање микрорганизама који су довели до инфекције и
микроорганизама чији су антигени коришћени приликом имунизације, затим статус животиње у
погледу инфекције приликом имунизације, могућност имуног одговора имунизоване јединке,
време имунизације с обзиром на технолошки процес узгоја и друго. Запажено је да се мења
приступ појединим микроорганизмима у односу на њихов значај. Испитивања у свету и у нашој
земљи указују на промене у етиологији, нарочито када су питању вируси. Питање је да ли су
промене у етиологији и оцени значаја стварне или се само услед побољшаних дијагностичких
могућности открива значај појединих узрочника који су раније били занемарени. Седамдесетих и
осамдесетих година прошлог века су се најзначајнијим вирусним узрочницима сматрали говеђи
херпес вирус 1 и вирус параинфлуенце 3, касније значај преузимају вирус дијареје говеда, говеђи
респираторни синцицијални вирус, а у новије време све више се запажа значај корона вируса
говеда. Истовремено, Mycoplasma bovis се такође почиње сматрати значајним узрочником
инфекција дисајних органа говеда. Бактеријске инфекције плућа су редован пратилац вирусних
инфекција, али у одређеним условима могу самостално да нанесу велику штету. Због тога се
сматра да је примена антибиотика неопходна и они се, када су у питању инфекције плућа, користе
поред терапијске и у профилактичку и метафилактичку сврху што повлачи за собом одговорност
ветеринара за контролисаном и одговорном употребом.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020
T1  - Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema
SP  - 51
EP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3520
ER  - 

@conference{
author = "Bugarski, Dejan and Petrović, Tamaš and Lupulović, Diana and Lazić, Sava and Todorović, Dalibor and Savić, Sara and Bojkovski, Jovan",
year = "2020",
abstract = "Инфекције дисајних органа говеда са својом мултифакторијалном етиологијом су сталан
проблем говедарске производње и истовремено изазов за ветеринарску струку. Најугроженије
узрасне и производне групе говеда, на фармама крава су телад старости до 4 месеца, а у
товилиштима телад на почетку това, најчешће у тежини између 100 и 250kg. На морбидитет, а
касније и на морталитет утичу услови држања, поступак са животињама, имуни статус,
изложеност микроорганизмима, врста присутних патогених микроорганизама.
Мултифакторијалност узрока овог обољења отежава постизање потпуне профилаксе те се пре у
овом случају може говорити о предузимању мера за смањење ризика и свођење трошкова које
узрокује обољење у прихватљив оквир. Штете које инфекције дисајних органа говеда наносе су
најизраженије у тову говеда. Са ветеринарског становишта, спровођење имунопрофилаксе се
сматра најделотворнијом и најлакшом методом у сузбијању респираторних инфекција говеда.
Међутим, у пракси се она не ретко показује као непотпуно успешна што доводи до незадовољства
власника животиња и његовог удаљавања од ветеринара. Узроци неуспеха спроведене
имунопрофилаксе су неподударање микрорганизама који су довели до инфекције и
микроорганизама чији су антигени коришћени приликом имунизације, затим статус животиње у
погледу инфекције приликом имунизације, могућност имуног одговора имунизоване јединке,
време имунизације с обзиром на технолошки процес узгоја и друго. Запажено је да се мења
приступ појединим микроорганизмима у односу на њихов значај. Испитивања у свету и у нашој
земљи указују на промене у етиологији, нарочито када су питању вируси. Питање је да ли су
промене у етиологији и оцени значаја стварне или се само услед побољшаних дијагностичких
могућности открива значај појединих узрочника који су раније били занемарени. Седамдесетих и
осамдесетих година прошлог века су се најзначајнијим вирусним узрочницима сматрали говеђи
херпес вирус 1 и вирус параинфлуенце 3, касније значај преузимају вирус дијареје говеда, говеђи
респираторни синцицијални вирус, а у новије време све више се запажа значај корона вируса
говеда. Истовремено, Mycoplasma bovis се такође почиње сматрати значајним узрочником
инфекција дисајних органа говеда. Бактеријске инфекције плућа су редован пратилац вирусних
инфекција, али у одређеним условима могу самостално да нанесу велику штету. Због тога се
сматра да је примена антибиотика неопходна и они се, када су у питању инфекције плућа, користе
поред терапијске и у профилактичку и метафилактичку сврху што повлачи за собом одговорност
ветеринара за контролисаном и одговорном употребом.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020",
title = "Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema",
pages = "51-62",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3520"
}

Bugarski, D., Petrović, T., Lupulović, D., Lazić, S., Todorović, D., Savić, S.,& Bojkovski, J.. (2020). Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema. in 31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020
Beograd : Srpsko veterinarsko društvo., 51-62.
https://hdl.handle.net/21.15107/rcub_veterinar_3520

Bugarski D, Petrović T, Lupulović D, Lazić S, Todorović D, Savić S, Bojkovski J. Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema. in 31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020. 2020;:51-62.
https://hdl.handle.net/21.15107/rcub_veterinar_3520 .

Bugarski, Dejan, Petrović, Tamaš, Lupulović, Diana, Lazić, Sava, Todorović, Dalibor, Savić, Sara, Bojkovski, Jovan, "Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema" in 31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020 (2020):51-62,
https://hdl.handle.net/21.15107/rcub_veterinar_3520 .

TY  - JOUR
AU  - Petrović, Tamaš
AU  - Šekler, Milanko
AU  - Petrić, Dusan
AU  - Lazić, Sava
AU  - Debeljak, Zoran
AU  - Vidanović, Dejan
AU  - Ignjatović-Čupina, Aleksandra
AU  - Lazić, Gospava
AU  - Lupulović, Diana
AU  - Kolarević, Migo
AU  - Plavsić, Budimir
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1632
AB  - Studies conducted during the past few years have confirmed active West Nile virus (WNV) circulation in Serbia. Based on these studies and the epidemiological situation, the Veterinary Directorate of the Ministry of Agriculture and Environmental Protection launched national WNV surveillance programmes in 2014 and 2015. The programmes encompassed the territory of Serbia and were conducted by the veterinary service in collaboration with entomologists and ornithologists. The objective of the programmes was early detection of WNV and timely reporting to the public health service and local authorities to increase both clinical and mosquito control preparedness. The WNV surveillance programmes were based on direct and indirect surveillance of the presence of WNV by the serological testing of initially seronegative sentinel horses and chickens as well as through viral detection in pooled mosquito and wild bird samples. The most intense WNV circulation was observed in all seven districts of Vojvodina Province (northern Serbia) and Belgrade City, where most of the positive samples were detected among sentinel animals, mosquitoes and wild birds. The West Nile virus surveillance programmes in 2014 and 2015 showed satisfactory results in their capacity to indicate the spatial distribution of the risk for humans and their sensitivity to early detect viral circulation at the enzootic level. Most of the human cases were preceded by the detection of WNV circulation as part of the surveillance programmes. According to the existing data, it can be reasonably assumed that WNV infection, now an endemic infection in Serbia, will continue to present a significant problem for the veterinary service and public health.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Methodology and results of integrated WNV surveillance programmes in Serbia
VL  - 13
IS  - 4
SP  - e0195439
DO  - 10.1371/journal.pone.0195439
ER  - 

@article{
author = "Petrović, Tamaš and Šekler, Milanko and Petrić, Dusan and Lazić, Sava and Debeljak, Zoran and Vidanović, Dejan and Ignjatović-Čupina, Aleksandra and Lazić, Gospava and Lupulović, Diana and Kolarević, Migo and Plavsić, Budimir",
year = "2018",
abstract = "Studies conducted during the past few years have confirmed active West Nile virus (WNV) circulation in Serbia. Based on these studies and the epidemiological situation, the Veterinary Directorate of the Ministry of Agriculture and Environmental Protection launched national WNV surveillance programmes in 2014 and 2015. The programmes encompassed the territory of Serbia and were conducted by the veterinary service in collaboration with entomologists and ornithologists. The objective of the programmes was early detection of WNV and timely reporting to the public health service and local authorities to increase both clinical and mosquito control preparedness. The WNV surveillance programmes were based on direct and indirect surveillance of the presence of WNV by the serological testing of initially seronegative sentinel horses and chickens as well as through viral detection in pooled mosquito and wild bird samples. The most intense WNV circulation was observed in all seven districts of Vojvodina Province (northern Serbia) and Belgrade City, where most of the positive samples were detected among sentinel animals, mosquitoes and wild birds. The West Nile virus surveillance programmes in 2014 and 2015 showed satisfactory results in their capacity to indicate the spatial distribution of the risk for humans and their sensitivity to early detect viral circulation at the enzootic level. Most of the human cases were preceded by the detection of WNV circulation as part of the surveillance programmes. According to the existing data, it can be reasonably assumed that WNV infection, now an endemic infection in Serbia, will continue to present a significant problem for the veterinary service and public health.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Methodology and results of integrated WNV surveillance programmes in Serbia",
volume = "13",
number = "4",
pages = "e0195439",
doi = "10.1371/journal.pone.0195439"
}

Petrović, T., Šekler, M., Petrić, D., Lazić, S., Debeljak, Z., Vidanović, D., Ignjatović-Čupina, A., Lazić, G., Lupulović, D., Kolarević, M.,& Plavsić, B.. (2018). Methodology and results of integrated WNV surveillance programmes in Serbia. in PLoS One
Public Library Science, San Francisco., 13(4), e0195439.
https://doi.org/10.1371/journal.pone.0195439

Petrović T, Šekler M, Petrić D, Lazić S, Debeljak Z, Vidanović D, Ignjatović-Čupina A, Lazić G, Lupulović D, Kolarević M, Plavsić B. Methodology and results of integrated WNV surveillance programmes in Serbia. in PLoS One. 2018;13(4):e0195439.
doi:10.1371/journal.pone.0195439 .

Petrović, Tamaš, Šekler, Milanko, Petrić, Dusan, Lazić, Sava, Debeljak, Zoran, Vidanović, Dejan, Ignjatović-Čupina, Aleksandra, Lazić, Gospava, Lupulović, Diana, Kolarević, Migo, Plavsić, Budimir, "Methodology and results of integrated WNV surveillance programmes in Serbia" in PLoS One, 13, no. 4 (2018):e0195439,
https://doi.org/10.1371/journal.pone.0195439 . .

TY  - CONF
AU  - Prodanov-Radulović, Jasna
AU  - Petrović, Tamaš
AU  - Stojanov, Igor
AU  - Lupulović, Diana
AU  - Maričić, Doroteja
AU  - Petrović, Jelena
AU  - Bojkovski, Jovan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3955
AB  - Mycoplasmal pneumonia caused by Mycoplasma hyopneumoniae (Mhyo) is an important
respiratory pig disease that causes severe economic losses to swine industry. It is
considered to be most common porcine respiratory disease in commercial swine farms,
with low mortality but high morbidity. The aim of research was to evaluate the diagnostic
process of mycoplasmal pleumonia caused by Mhyo and implementation control measures
at a herd level. The research methods included clinical and gross pathological examination
of diseased and dead pigs, and control of respiratory organs at the slaughterhouse. The
material for examination included lungs, tonsils and lymph node samples of dead animals
and fatteners obtained at slaughter line (360 samples) and blood samples from selected
animals (75 samples). The following research laboratory methods were applied: standard
bacteriological diagnostic methods (aerobic and microaerophilic cultivation) and molecular
diagnostic methods (RT-PCR) for Mhyo. Serum samples were examined using
immunoenzyme (ELISA) test for Mhyo (IDEXX M. hyo). By examination of respiratory
organs at the slaughter line, no visible changes in lung tissue were observed only in 19.44%
of examined pigs. In others, examination of respiratory organs frequently revealed
pathological changes indicative for Mhyo infection. Serological examination revealed
presence of specific antibodies against Mhyo in 88% o f t ested s era s amples. B y
bacteriological examination the following bacteria was detected: Haemophilus parasuis,
Pasteurella multocida, Staphylococcus aureus, Mannheimia haemolytica. The obtained
results suggest the necessity of implementation of an updated medication and vaccination
program, taking into consideration the organization structure of farrow-to-finish herds in
the region.
PB  - Belgrade : Faculty of Agriculture
C3  - International Symposium on Animal Science (ISAS), Belgrade, 24 - 25 November, 2016
T1  - Diagnostics and control of mycoplasmal pneumonia (Mycoplasma hyopneumoniae) in farrow-to-finish swine herds
SP  - 360
EP  - 366
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3955
ER  - 

@conference{
author = "Prodanov-Radulović, Jasna and Petrović, Tamaš and Stojanov, Igor and Lupulović, Diana and Maričić, Doroteja and Petrović, Jelena and Bojkovski, Jovan",
year = "2016",
abstract = "Mycoplasmal pneumonia caused by Mycoplasma hyopneumoniae (Mhyo) is an important
respiratory pig disease that causes severe economic losses to swine industry. It is
considered to be most common porcine respiratory disease in commercial swine farms,
with low mortality but high morbidity. The aim of research was to evaluate the diagnostic
process of mycoplasmal pleumonia caused by Mhyo and implementation control measures
at a herd level. The research methods included clinical and gross pathological examination
of diseased and dead pigs, and control of respiratory organs at the slaughterhouse. The
material for examination included lungs, tonsils and lymph node samples of dead animals
and fatteners obtained at slaughter line (360 samples) and blood samples from selected
animals (75 samples). The following research laboratory methods were applied: standard
bacteriological diagnostic methods (aerobic and microaerophilic cultivation) and molecular
diagnostic methods (RT-PCR) for Mhyo. Serum samples were examined using
immunoenzyme (ELISA) test for Mhyo (IDEXX M. hyo). By examination of respiratory
organs at the slaughter line, no visible changes in lung tissue were observed only in 19.44%
of examined pigs. In others, examination of respiratory organs frequently revealed
pathological changes indicative for Mhyo infection. Serological examination revealed
presence of specific antibodies against Mhyo in 88% o f t ested s era s amples. B y
bacteriological examination the following bacteria was detected: Haemophilus parasuis,
Pasteurella multocida, Staphylococcus aureus, Mannheimia haemolytica. The obtained
results suggest the necessity of implementation of an updated medication and vaccination
program, taking into consideration the organization structure of farrow-to-finish herds in
the region.",
publisher = "Belgrade : Faculty of Agriculture",
journal = "International Symposium on Animal Science (ISAS), Belgrade, 24 - 25 November, 2016",
title = "Diagnostics and control of mycoplasmal pneumonia (Mycoplasma hyopneumoniae) in farrow-to-finish swine herds",
pages = "360-366",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3955"
}

Prodanov-Radulović, J., Petrović, T., Stojanov, I., Lupulović, D., Maričić, D., Petrović, J.,& Bojkovski, J.. (2016). Diagnostics and control of mycoplasmal pneumonia (Mycoplasma hyopneumoniae) in farrow-to-finish swine herds. in International Symposium on Animal Science (ISAS), Belgrade, 24 - 25 November, 2016
Belgrade : Faculty of Agriculture., 360-366.
https://hdl.handle.net/21.15107/rcub_veterinar_3955

Prodanov-Radulović J, Petrović T, Stojanov I, Lupulović D, Maričić D, Petrović J, Bojkovski J. Diagnostics and control of mycoplasmal pneumonia (Mycoplasma hyopneumoniae) in farrow-to-finish swine herds. in International Symposium on Animal Science (ISAS), Belgrade, 24 - 25 November, 2016. 2016;:360-366.
https://hdl.handle.net/21.15107/rcub_veterinar_3955 .

Prodanov-Radulović, Jasna, Petrović, Tamaš, Stojanov, Igor, Lupulović, Diana, Maričić, Doroteja, Petrović, Jelena, Bojkovski, Jovan, "Diagnostics and control of mycoplasmal pneumonia (Mycoplasma hyopneumoniae) in farrow-to-finish swine herds" in International Symposium on Animal Science (ISAS), Belgrade, 24 - 25 November, 2016 (2016):360-366,
https://hdl.handle.net/21.15107/rcub_veterinar_3955 .

TY  - JOUR
AU  - Lazić, Sava
AU  - Lupulović, Diana
AU  - Polaček, Vladimir
AU  - Valčić, Miroslav
AU  - Lazić, Gospava
AU  - Pasagić, Enisa
AU  - Petrović, Tamaš
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1180
AB  - The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specific antibodies in blood sera was performed by the virus neutralization test (VNT), and identification of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log 2. High seroprevalence was confirmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while EAV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confirmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specific antibodies was confirmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Detection of equine arteritis virus in the semen of stallions in the republic of Serbia
VL  - 65
IS  - 4
SP  - 557
EP  - 567
DO  - 10.1515/acve-2015-0047
ER  - 

@article{
author = "Lazić, Sava and Lupulović, Diana and Polaček, Vladimir and Valčić, Miroslav and Lazić, Gospava and Pasagić, Enisa and Petrović, Tamaš",
year = "2015",
abstract = "The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specific antibodies in blood sera was performed by the virus neutralization test (VNT), and identification of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log 2. High seroprevalence was confirmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while EAV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confirmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specific antibodies was confirmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Detection of equine arteritis virus in the semen of stallions in the republic of Serbia",
volume = "65",
number = "4",
pages = "557-567",
doi = "10.1515/acve-2015-0047"
}

Lazić, S., Lupulović, D., Polaček, V., Valčić, M., Lazić, G., Pasagić, E.,& Petrović, T.. (2015). Detection of equine arteritis virus in the semen of stallions in the republic of Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(4), 557-567.
https://doi.org/10.1515/acve-2015-0047

Lazić S, Lupulović D, Polaček V, Valčić M, Lazić G, Pasagić E, Petrović T. Detection of equine arteritis virus in the semen of stallions in the republic of Serbia. in Acta Veterinaria-Beograd. 2015;65(4):557-567.
doi:10.1515/acve-2015-0047 .

Lazić, Sava, Lupulović, Diana, Polaček, Vladimir, Valčić, Miroslav, Lazić, Gospava, Pasagić, Enisa, Petrović, Tamaš, "Detection of equine arteritis virus in the semen of stallions in the republic of Serbia" in Acta Veterinaria-Beograd, 65, no. 4 (2015):557-567,
https://doi.org/10.1515/acve-2015-0047 . .

TY  - JOUR
AU  - Petrović, Tamaš
AU  - Lupulović, Diana
AU  - Petrić, Dušan
AU  - Vasić, Ana
AU  - Hrnjaković-Cvjetković, Ivana
AU  - Milošević, Vesna
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Lazić, Sava
AU  - Đuričić, Bosiljka
AU  - Plavšić, Budimir
AU  - Juan-Carlos, Saiz
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1205
AB  - West Nile virus (WNV) is a neurovirulent mosquito-borne Flavivirus with zoonotic potential. Virus is maintained in nature in an enzootic transmission cycle between avian hosts and mosquito vectors, but occasionally infects other vertebrates. The infection in horses and humans can be asymptomatic or it can have different clinical manifestations ranging from light febrile diseases to fatal meningoencephalitis. Recently, the number, frequency and severity of outbreaks with neurological consequences for birds, humans and horses have increased dramatically throughout central and south Europe, including Serbia, posing a serious veterinary and public health problem. The emergency of WNV infections in Serbia is described through the current epidemiology situation based on recent data on the incidence of WNV infection among virus natural hosts and vectors; sentinel (horses) and other animal species, and in human population. The results of the WNV serology studies conducted on horse blood samples collected in different occasions during the last six years, and the results of the serology studies conducted among other animal species like pigs, wild boars, roe deer and dogs in Serbia are presented and discussed. Also, the results of the first studies on WNV presence in mosquito vectors and in wild birds as virus natural hosts in Serbia are presented and analyzed. In addition, the data on the WNV serology studies conducted in human population in Serbia in the last few years, and the existing data of WNV outbreaks in 2012 and 2013 are included. Regarding the existing knowledge on WNV epidemiology situation, the crucial role of veterinary service in early detection of WNV presence and ongoing national program of WNV surveillance in sentinel animals, mosquitoes and wild birds are discussed.
AB  - Virus Zapadnog Nila (VZN) je neurovirulentni, komarcima prenosivi flavivirus zoonotskog potencijala. Virus se održava u prirodi u enzootskom ciklusu kruženja između ptica kao domaćina i komaraca kao vektora virusa ali povremeno može inficirati i druge kičmenjake. Infekcija ljudi i konja može proći asimptomatično ili sa različitom kliničkom slikom od blagog febrilnog stanja do letalnog meningoencefalitisa. Poslednjih godina u centralnoj i južnoj Evropi, kao i u Srbiji je dramatično povećan broj, učestalost i težina oboljenja sa neurološkim poremećajima kod ptica, ljudi i konja, predstavljući značajan problem veterinarskog i javnog zdravlja. Značaj problema infekcije VZN u Srbiji je opisan preko trenutne epizootiološko-epidemiološke situacije bazirane na skorašnjim podacima o prisustvu ove infekcije kod prirodnih domaćina i vektora, kod konja kao sentinel životinja i drugih vrsta životinja, kao i u humanoj populaciji. Opisani su rezultati seroloških ispitivanja sprovedenih u Srbiji na uzorcima krvnih seruma konja uzorkovanih u različitim periodima tokom poslednjih 6 godina, kao i rezultati seroloških ispitivanja sprovedenih na uzorcima krvnih seruma drugih životinjskih vrsta, kao što su svinje, divlje svinje, srndaći i psi. Takođe, prikazani su i analizirani rezultati prvih istraživanja prisustva virusa u komarcima vektorima i divljim pticama kao prirodnim domaćinima u Srbiji. Pored toga, predstavljeni su i rezultati seroloških istraživanja sprovedenih u humanoj populaciji u Srbiji tokom poslednjih nekoliko godina, kao i podaci o epidemijama u 2012. i 2013. godini. Na osnovu postojećeg znanja o epidemiološkoj situaciji, istaknuta je presudna uloga veterinarske službe u ranoj detekciji prisustva VZN, kao i tekući nacionalni program nadzora prisustva VZN kod sentinel životinja, komaraca i divljih ptica u Srbiji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - WNV infection: An emergent vector borne viral infection in Serbia: Current situation
T1  - Groznica zapadnog Nila - značajna vektorska virusna infekcija u Srbiji - aktuelna situacija
VL  - 69
IS  - 1-2
SP  - 111
EP  - 126
DO  - 10.2298/VETGL1502111P
ER  - 

@article{
author = "Petrović, Tamaš and Lupulović, Diana and Petrić, Dušan and Vasić, Ana and Hrnjaković-Cvjetković, Ivana and Milošević, Vesna and Vidanović, Dejan and Šekler, Milanko and Lazić, Sava and Đuričić, Bosiljka and Plavšić, Budimir and Juan-Carlos, Saiz",
year = "2015",
abstract = "West Nile virus (WNV) is a neurovirulent mosquito-borne Flavivirus with zoonotic potential. Virus is maintained in nature in an enzootic transmission cycle between avian hosts and mosquito vectors, but occasionally infects other vertebrates. The infection in horses and humans can be asymptomatic or it can have different clinical manifestations ranging from light febrile diseases to fatal meningoencephalitis. Recently, the number, frequency and severity of outbreaks with neurological consequences for birds, humans and horses have increased dramatically throughout central and south Europe, including Serbia, posing a serious veterinary and public health problem. The emergency of WNV infections in Serbia is described through the current epidemiology situation based on recent data on the incidence of WNV infection among virus natural hosts and vectors; sentinel (horses) and other animal species, and in human population. The results of the WNV serology studies conducted on horse blood samples collected in different occasions during the last six years, and the results of the serology studies conducted among other animal species like pigs, wild boars, roe deer and dogs in Serbia are presented and discussed. Also, the results of the first studies on WNV presence in mosquito vectors and in wild birds as virus natural hosts in Serbia are presented and analyzed. In addition, the data on the WNV serology studies conducted in human population in Serbia in the last few years, and the existing data of WNV outbreaks in 2012 and 2013 are included. Regarding the existing knowledge on WNV epidemiology situation, the crucial role of veterinary service in early detection of WNV presence and ongoing national program of WNV surveillance in sentinel animals, mosquitoes and wild birds are discussed., Virus Zapadnog Nila (VZN) je neurovirulentni, komarcima prenosivi flavivirus zoonotskog potencijala. Virus se održava u prirodi u enzootskom ciklusu kruženja između ptica kao domaćina i komaraca kao vektora virusa ali povremeno može inficirati i druge kičmenjake. Infekcija ljudi i konja može proći asimptomatično ili sa različitom kliničkom slikom od blagog febrilnog stanja do letalnog meningoencefalitisa. Poslednjih godina u centralnoj i južnoj Evropi, kao i u Srbiji je dramatično povećan broj, učestalost i težina oboljenja sa neurološkim poremećajima kod ptica, ljudi i konja, predstavljući značajan problem veterinarskog i javnog zdravlja. Značaj problema infekcije VZN u Srbiji je opisan preko trenutne epizootiološko-epidemiološke situacije bazirane na skorašnjim podacima o prisustvu ove infekcije kod prirodnih domaćina i vektora, kod konja kao sentinel životinja i drugih vrsta životinja, kao i u humanoj populaciji. Opisani su rezultati seroloških ispitivanja sprovedenih u Srbiji na uzorcima krvnih seruma konja uzorkovanih u različitim periodima tokom poslednjih 6 godina, kao i rezultati seroloških ispitivanja sprovedenih na uzorcima krvnih seruma drugih životinjskih vrsta, kao što su svinje, divlje svinje, srndaći i psi. Takođe, prikazani su i analizirani rezultati prvih istraživanja prisustva virusa u komarcima vektorima i divljim pticama kao prirodnim domaćinima u Srbiji. Pored toga, predstavljeni su i rezultati seroloških istraživanja sprovedenih u humanoj populaciji u Srbiji tokom poslednjih nekoliko godina, kao i podaci o epidemijama u 2012. i 2013. godini. Na osnovu postojećeg znanja o epidemiološkoj situaciji, istaknuta je presudna uloga veterinarske službe u ranoj detekciji prisustva VZN, kao i tekući nacionalni program nadzora prisustva VZN kod sentinel životinja, komaraca i divljih ptica u Srbiji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "WNV infection: An emergent vector borne viral infection in Serbia: Current situation, Groznica zapadnog Nila - značajna vektorska virusna infekcija u Srbiji - aktuelna situacija",
volume = "69",
number = "1-2",
pages = "111-126",
doi = "10.2298/VETGL1502111P"
}

Petrović, T., Lupulović, D., Petrić, D., Vasić, A., Hrnjaković-Cvjetković, I., Milošević, V., Vidanović, D., Šekler, M., Lazić, S., Đuričić, B., Plavšić, B.,& Juan-Carlos, S.. (2015). WNV infection: An emergent vector borne viral infection in Serbia: Current situation. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(1-2), 111-126.
https://doi.org/10.2298/VETGL1502111P

Petrović T, Lupulović D, Petrić D, Vasić A, Hrnjaković-Cvjetković I, Milošević V, Vidanović D, Šekler M, Lazić S, Đuričić B, Plavšić B, Juan-Carlos S. WNV infection: An emergent vector borne viral infection in Serbia: Current situation. in Veterinarski Glasnik. 2015;69(1-2):111-126.
doi:10.2298/VETGL1502111P .

Petrović, Tamaš, Lupulović, Diana, Petrić, Dušan, Vasić, Ana, Hrnjaković-Cvjetković, Ivana, Milošević, Vesna, Vidanović, Dejan, Šekler, Milanko, Lazić, Sava, Đuričić, Bosiljka, Plavšić, Budimir, Juan-Carlos, Saiz, "WNV infection: An emergent vector borne viral infection in Serbia: Current situation" in Veterinarski Glasnik, 69, no. 1-2 (2015):111-126,
https://doi.org/10.2298/VETGL1502111P . .

TY  - JOUR
AU  - Toplak, Ivan
AU  - Lazić, Sava
AU  - Lupulović, Diana
AU  - Prodanov-Radulović, Jasna
AU  - Becskei, Zsolt
AU  - Došen, Radoslav
AU  - Petrović, Tamaš
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/936
AB  - Recent variants of porcine circovirus type 2 (PCV2) were obtained from tissues of domestic pigs with porcine circovirus associated disease and from randomly selected wild boar samples from Serbia and Slovenia. A 450-base-pair nucleotide sequence was obtained by PCR from the ORF2. The derived nucleotide and amino acid sequences were aligned and compared to the corresponding region of closely related PCV2 sequences determined in previous years and retrieved from the GenBank. The 30 Serbian and 17 Slovenian PCV2 sequences clustered into three previously determined genotypes (PCV2a: 7), (PCV2b: 38) and (PCV2d: 2). Three major variable regions, concerning 29 amino acid position substitutions within the ORF2, were observed, which further supports the segregation of the detected strains into three separate genotypes. This study indicates that PCV2b is the predominant genotype in Serbia and Slovenia and the detected PCV2 strains are closely related to those previously described in Europe and in other parts of the world.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia
VL  - 60
IS  - 3
SP  - 409
EP  - 420
DO  - 10.1556/AVet.2012.035
ER  - 

@article{
author = "Toplak, Ivan and Lazić, Sava and Lupulović, Diana and Prodanov-Radulović, Jasna and Becskei, Zsolt and Došen, Radoslav and Petrović, Tamaš",
year = "2012",
abstract = "Recent variants of porcine circovirus type 2 (PCV2) were obtained from tissues of domestic pigs with porcine circovirus associated disease and from randomly selected wild boar samples from Serbia and Slovenia. A 450-base-pair nucleotide sequence was obtained by PCR from the ORF2. The derived nucleotide and amino acid sequences were aligned and compared to the corresponding region of closely related PCV2 sequences determined in previous years and retrieved from the GenBank. The 30 Serbian and 17 Slovenian PCV2 sequences clustered into three previously determined genotypes (PCV2a: 7), (PCV2b: 38) and (PCV2d: 2). Three major variable regions, concerning 29 amino acid position substitutions within the ORF2, were observed, which further supports the segregation of the detected strains into three separate genotypes. This study indicates that PCV2b is the predominant genotype in Serbia and Slovenia and the detected PCV2 strains are closely related to those previously described in Europe and in other parts of the world.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia",
volume = "60",
number = "3",
pages = "409-420",
doi = "10.1556/AVet.2012.035"
}

Toplak, I., Lazić, S., Lupulović, D., Prodanov-Radulović, J., Becskei, Z., Došen, R.,& Petrović, T.. (2012). Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia. in Acta Veterinaria Hungarica
Akademiai Kiado Zrt, Budapest., 60(3), 409-420.
https://doi.org/10.1556/AVet.2012.035

Toplak I, Lazić S, Lupulović D, Prodanov-Radulović J, Becskei Z, Došen R, Petrović T. Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia. in Acta Veterinaria Hungarica. 2012;60(3):409-420.
doi:10.1556/AVet.2012.035 .

Toplak, Ivan, Lazić, Sava, Lupulović, Diana, Prodanov-Radulović, Jasna, Becskei, Zsolt, Došen, Radoslav, Petrović, Tamaš, "Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia" in Acta Veterinaria Hungarica, 60, no. 3 (2012):409-420,
https://doi.org/10.1556/AVet.2012.035 . .

TY  - JOUR
AU  - Lazić, Sava
AU  - Rogan, Dragan
AU  - Petrović, Tamaš
AU  - Bugarski, Dejan
AU  - Lupulović, Diana
AU  - Lazarević, Miodrag
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/723
AB  - The paper presents the results of investigations of the presence of the bovine herpesvirus-1 (BHV-1) in samples of blood serum from 106 cows and 107 of their calves (one cow had twins). Blood was sampled from the cows immediately after parturition, and from the calves before feeding on colostrum. The examined cows and their calves originated from 5 herds in which previous investigations had shown infection with the bovine herpesvirus-1. The determination of antibodies against BHV-1 was performed using the method of virus neutralization in culture of MDBK cells with 100 TCID/50 viruses (BHV-1, TN-41 Am. Bio Research, USA). Antibodies against BHV-1 were determined in all blood serum samples of cows and in 16 samples of precolostral blood serums of calves. The antibody titer values in cows ranged from 1:4 to 1:512, and in calves the determined values were from 1:2 to 1:16. The results indicate that cows that are seropositive to BHV-1 can deliver calves seropositive to BHV-1 in about 15% cases. This must be kept in mind in selecting cows for the production of breeding material, in particular bulls for reproduction centers, as well as in making a programme for the immunoprophylaxis of calves against BHV-1. .
AB  - U radu su prikazani rezultati ispitivanja prisustva antitela protiv goveđeg herpesvirusa-1 (BHV-1) u uzorcima krvnih seruma 106 krava i 107 njihove teladi (jedna krava je otelila blizance). Uzorkovanje krvi od krava je vršeno odmah nakon partusa, a od teladi pre ishrane kolostrumom. Ispitivane krave i njihova telad poticali su iz 5 zapata u kojima je ranijim ispitivanjima utvrđena infekcija goveđim herpesvirusom-1. Utvrđivanje antitela protiv BHV-1 vršeno je metodom virus neutralizacije na kulturi MDBK ćelija sa 100 TCID/50 virusa (BHV-1, TN-41 Am. Bio Research, USA). Antitela protiv BHV-1 utvrđena su u svim uzorcima krvnih seruma krava i u 16 uzoraka prekolostralnih krvnih seruma teladi. Vrednosti titra antitela iznosile su kod krava od 1:4 do 1:512, a kod teladi od 1:2 do 1:16. Rezultati ukazuju na to da krave koje su seropozitivne na BHV-1 mogu da otele telad seropozitivnu na BHV-1 u oko 15% slučajeva. Ovo se mora imati u vidu kada se vrši izbor krava za proizvodnju priplodnog materijala, a posebno bikova za reprocentre, kao i kada se pravi program imunoprofilakse teladi protiv BHV-1. .
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Investigations of presence of antibodies against bovine herpesvirus-1 in blood serum of calves prior to colostrum diet
T1  - Ispitivanje prisustva antitela protiv goveđeg herpesvirusa-1 u krvnom serumu teladi pre ishrane kolostrumom
VL  - 64
IS  - 1-2
SP  - 33
EP  - 41
DO  - 10.2298/VETGL1002033L
ER  - 

@article{
author = "Lazić, Sava and Rogan, Dragan and Petrović, Tamaš and Bugarski, Dejan and Lupulović, Diana and Lazarević, Miodrag",
year = "2010",
abstract = "The paper presents the results of investigations of the presence of the bovine herpesvirus-1 (BHV-1) in samples of blood serum from 106 cows and 107 of their calves (one cow had twins). Blood was sampled from the cows immediately after parturition, and from the calves before feeding on colostrum. The examined cows and their calves originated from 5 herds in which previous investigations had shown infection with the bovine herpesvirus-1. The determination of antibodies against BHV-1 was performed using the method of virus neutralization in culture of MDBK cells with 100 TCID/50 viruses (BHV-1, TN-41 Am. Bio Research, USA). Antibodies against BHV-1 were determined in all blood serum samples of cows and in 16 samples of precolostral blood serums of calves. The antibody titer values in cows ranged from 1:4 to 1:512, and in calves the determined values were from 1:2 to 1:16. The results indicate that cows that are seropositive to BHV-1 can deliver calves seropositive to BHV-1 in about 15% cases. This must be kept in mind in selecting cows for the production of breeding material, in particular bulls for reproduction centers, as well as in making a programme for the immunoprophylaxis of calves against BHV-1. ., U radu su prikazani rezultati ispitivanja prisustva antitela protiv goveđeg herpesvirusa-1 (BHV-1) u uzorcima krvnih seruma 106 krava i 107 njihove teladi (jedna krava je otelila blizance). Uzorkovanje krvi od krava je vršeno odmah nakon partusa, a od teladi pre ishrane kolostrumom. Ispitivane krave i njihova telad poticali su iz 5 zapata u kojima je ranijim ispitivanjima utvrđena infekcija goveđim herpesvirusom-1. Utvrđivanje antitela protiv BHV-1 vršeno je metodom virus neutralizacije na kulturi MDBK ćelija sa 100 TCID/50 virusa (BHV-1, TN-41 Am. Bio Research, USA). Antitela protiv BHV-1 utvrđena su u svim uzorcima krvnih seruma krava i u 16 uzoraka prekolostralnih krvnih seruma teladi. Vrednosti titra antitela iznosile su kod krava od 1:4 do 1:512, a kod teladi od 1:2 do 1:16. Rezultati ukazuju na to da krave koje su seropozitivne na BHV-1 mogu da otele telad seropozitivnu na BHV-1 u oko 15% slučajeva. Ovo se mora imati u vidu kada se vrši izbor krava za proizvodnju priplodnog materijala, a posebno bikova za reprocentre, kao i kada se pravi program imunoprofilakse teladi protiv BHV-1. .",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Investigations of presence of antibodies against bovine herpesvirus-1 in blood serum of calves prior to colostrum diet, Ispitivanje prisustva antitela protiv goveđeg herpesvirusa-1 u krvnom serumu teladi pre ishrane kolostrumom",
volume = "64",
number = "1-2",
pages = "33-41",
doi = "10.2298/VETGL1002033L"
}

Lazić, S., Rogan, D., Petrović, T., Bugarski, D., Lupulović, D.,& Lazarević, M.. (2010). Investigations of presence of antibodies against bovine herpesvirus-1 in blood serum of calves prior to colostrum diet. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(1-2), 33-41.
https://doi.org/10.2298/VETGL1002033L

Lazić S, Rogan D, Petrović T, Bugarski D, Lupulović D, Lazarević M. Investigations of presence of antibodies against bovine herpesvirus-1 in blood serum of calves prior to colostrum diet. in Veterinarski Glasnik. 2010;64(1-2):33-41.
doi:10.2298/VETGL1002033L .

Lazić, Sava, Rogan, Dragan, Petrović, Tamaš, Bugarski, Dejan, Lupulović, Diana, Lazarević, Miodrag, "Investigations of presence of antibodies against bovine herpesvirus-1 in blood serum of calves prior to colostrum diet" in Veterinarski Glasnik, 64, no. 1-2 (2010):33-41,
https://doi.org/10.2298/VETGL1002033L . .

TY  - JOUR
AU  - Lupulović, Diana
AU  - Milić, Nenad
AU  - Petrović, Tamaš
AU  - Prodanov, Jasna
AU  - Lazić, Sava
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/492
AB  - Parvoviral infection of swine is a disease which is manifested in reproductive disorders in sows and gilts in the form of anestria, premature births, miscarriages, mummified fetuses, the birth of poorly vital piglets and/or a reduced number of piglets in the litter. The infection is present in farms with intensive breeding conditions in the form of an endemic infection, all over the world, and also in our country. Timely diagnostics and adequate prophylaxis prevent the occurrence and spread of this disease. Experimental investigations covered 21 sows, divided into two experimental and a third, the control, group. Animals of the first experimental group were vaccinated once before exposure to the boar using an inactivated vaccine, Porcilis Parvo, and animals of the second experimental group were vaccinated twice at an interval of 3 weeks, also using an inactivated vaccine, Parvovax. Sows of the control group were not vaccinated. Blood samples were taken from all animals four times during the course of the experiment, and specific antibodies against the swine parvovirus were determined using the method of hemagglutination inhibition (HI test). The results of the investigations indicate that there was an increase in the titre of specific antibodies following the vaccination of persistently infected sows with the swine parvivirus, and that the present antibodies did not prevent the creating of an immune response. It was established following a comparison of the geometric mean values of antibody titres of vaccinated sows that there was a significant increase in the antibody level following the two vaccinations using the Parvovax vaccine, against the titre values in sows vaccinated once with the Porcilis Parvo vaccine. In control animals, the average value of the antibody level was many times lower in comparison with the established values in the experimental groups. This provides justification for the implementation of immunoprophylaxis against swine parvovirosis by the vaccination of sows and gilts before mating using inactivated vaccines.
AB  - Parvovirusna infekcija svinja je oboljenje koje se manifestuje reproduktivnim poremećajima kod krmača i nazimica u vidu anestrija, prevremenih porođaja, pobačaja, mumifikacijom plodova, rađanjem slabovitalne prasadi i/ili smanjenim brojem prasadi u leglu. Infekcija je na farmama sa intezivnim uzgojem svinja prisutna u vidu endemske infekcije, kako u svetu tako i kod nas. Sprečavanje pojave i širenja ovog obolenja obezbeđuju pravovremena dijagnostika i adekvatna imunoprofilaksa. Eksperimentalnim ispitivanjem obuhvaćena je 21 krmača, koje su svrstane u dve ogledne i treću, kontrolnu grupu. Prva ogledna grupa životinja vakcinisana je pre pripusta jednokratno, inaktivisanom vakcinom Porcilis Parvo, a druga ogledna grupa dvokratno, u intervalu od 3 nedelje, takođe inaktivisanom vakcinom Parvovax. Krmače kontrolne grupe nisu vakcinisane. Uzorkovanje krvi svih životinja vršeno je 4 puta tokom trajanja ogleda, a utvrđivanje specifičnih antitela protiv parvovirusa svinja vršeno je metodom inhibicije hemaglutinacije (HI test). Rezultati ispitivanja ukazuju na porast titra specifičnih antitela posle vakcinacije perzistentno inficiranih krmača parvovirusom svinja, odnosno prisutna antitela nisu sprečila stvaranje imunološkog odgovora. Poređenjem geometrijskih srednjih vrednosti titra antitela vakcinisanih krmača, ustanovljeno je da je značajan rast nivoa antitela nastao posle dvokratne vakcinacije Parvovax vakcinom u odnosu na vrednosti titra kod krmača koje su vakcinisane Porcilis Parvo vakcinom jednokratno. Kod kontrolnih životinja, prosečna vrednost nivoa antitela bila je višestruko niža u poređenju sa utvrđenim vrednostima kod oglednih grupa. Time se smatra opravdanim sprovođenje imunoprofilakse protiv parvoviroze svinja putem vakcinacije krmača i nazimica pre pripusta, inaktivisanim vakcinama.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Investigations of significance of vaccination against swine parvovirosis in persistently infected sows
T1  - Ispitivanje značaja vakcinacije protiv parvoviroze svinja kod perzistentno inficiranih krmača
VL  - 61
IS  - 5-6
SP  - 251
EP  - 260
DO  - 10.2298/VETGL0706251L
ER  - 

@article{
author = "Lupulović, Diana and Milić, Nenad and Petrović, Tamaš and Prodanov, Jasna and Lazić, Sava",
year = "2007",
abstract = "Parvoviral infection of swine is a disease which is manifested in reproductive disorders in sows and gilts in the form of anestria, premature births, miscarriages, mummified fetuses, the birth of poorly vital piglets and/or a reduced number of piglets in the litter. The infection is present in farms with intensive breeding conditions in the form of an endemic infection, all over the world, and also in our country. Timely diagnostics and adequate prophylaxis prevent the occurrence and spread of this disease. Experimental investigations covered 21 sows, divided into two experimental and a third, the control, group. Animals of the first experimental group were vaccinated once before exposure to the boar using an inactivated vaccine, Porcilis Parvo, and animals of the second experimental group were vaccinated twice at an interval of 3 weeks, also using an inactivated vaccine, Parvovax. Sows of the control group were not vaccinated. Blood samples were taken from all animals four times during the course of the experiment, and specific antibodies against the swine parvovirus were determined using the method of hemagglutination inhibition (HI test). The results of the investigations indicate that there was an increase in the titre of specific antibodies following the vaccination of persistently infected sows with the swine parvivirus, and that the present antibodies did not prevent the creating of an immune response. It was established following a comparison of the geometric mean values of antibody titres of vaccinated sows that there was a significant increase in the antibody level following the two vaccinations using the Parvovax vaccine, against the titre values in sows vaccinated once with the Porcilis Parvo vaccine. In control animals, the average value of the antibody level was many times lower in comparison with the established values in the experimental groups. This provides justification for the implementation of immunoprophylaxis against swine parvovirosis by the vaccination of sows and gilts before mating using inactivated vaccines., Parvovirusna infekcija svinja je oboljenje koje se manifestuje reproduktivnim poremećajima kod krmača i nazimica u vidu anestrija, prevremenih porođaja, pobačaja, mumifikacijom plodova, rađanjem slabovitalne prasadi i/ili smanjenim brojem prasadi u leglu. Infekcija je na farmama sa intezivnim uzgojem svinja prisutna u vidu endemske infekcije, kako u svetu tako i kod nas. Sprečavanje pojave i širenja ovog obolenja obezbeđuju pravovremena dijagnostika i adekvatna imunoprofilaksa. Eksperimentalnim ispitivanjem obuhvaćena je 21 krmača, koje su svrstane u dve ogledne i treću, kontrolnu grupu. Prva ogledna grupa životinja vakcinisana je pre pripusta jednokratno, inaktivisanom vakcinom Porcilis Parvo, a druga ogledna grupa dvokratno, u intervalu od 3 nedelje, takođe inaktivisanom vakcinom Parvovax. Krmače kontrolne grupe nisu vakcinisane. Uzorkovanje krvi svih životinja vršeno je 4 puta tokom trajanja ogleda, a utvrđivanje specifičnih antitela protiv parvovirusa svinja vršeno je metodom inhibicije hemaglutinacije (HI test). Rezultati ispitivanja ukazuju na porast titra specifičnih antitela posle vakcinacije perzistentno inficiranih krmača parvovirusom svinja, odnosno prisutna antitela nisu sprečila stvaranje imunološkog odgovora. Poređenjem geometrijskih srednjih vrednosti titra antitela vakcinisanih krmača, ustanovljeno je da je značajan rast nivoa antitela nastao posle dvokratne vakcinacije Parvovax vakcinom u odnosu na vrednosti titra kod krmača koje su vakcinisane Porcilis Parvo vakcinom jednokratno. Kod kontrolnih životinja, prosečna vrednost nivoa antitela bila je višestruko niža u poređenju sa utvrđenim vrednostima kod oglednih grupa. Time se smatra opravdanim sprovođenje imunoprofilakse protiv parvoviroze svinja putem vakcinacije krmača i nazimica pre pripusta, inaktivisanim vakcinama.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Investigations of significance of vaccination against swine parvovirosis in persistently infected sows, Ispitivanje značaja vakcinacije protiv parvoviroze svinja kod perzistentno inficiranih krmača",
volume = "61",
number = "5-6",
pages = "251-260",
doi = "10.2298/VETGL0706251L"
}

Lupulović, D., Milić, N., Petrović, T., Prodanov, J.,& Lazić, S.. (2007). Investigations of significance of vaccination against swine parvovirosis in persistently infected sows. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(5-6), 251-260.
https://doi.org/10.2298/VETGL0706251L

Lupulović D, Milić N, Petrović T, Prodanov J, Lazić S. Investigations of significance of vaccination against swine parvovirosis in persistently infected sows. in Veterinarski Glasnik. 2007;61(5-6):251-260.
doi:10.2298/VETGL0706251L .

Lupulović, Diana, Milić, Nenad, Petrović, Tamaš, Prodanov, Jasna, Lazić, Sava, "Investigations of significance of vaccination against swine parvovirosis in persistently infected sows" in Veterinarski Glasnik, 61, no. 5-6 (2007):251-260,
https://doi.org/10.2298/VETGL0706251L . .

TY  - JOUR
AU  - Prodanov, Jasna
AU  - Došen, Radoslav
AU  - Petrović, Tamaš
AU  - Lupulović, Diana
AU  - Valčić, Miroslav
AU  - Polaček, Vladimir
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/490
AB  - Intrauterine infection of the fetus occurs if a pregnant non-vaccinated sow is infected with the virus of classical swine plague (CSF). The infection of the fetus results in the occurrence of viremia and the distribution of the virus in fetal tissue is similar to the distribution which is established in post-natal infected swine. The objective of these investigations was to determine intrauterine (transplacental) infection in the event of the appearance of CSF in different periods of pregnancy in non-vaccinated and vaccinated sows. The examined material were organs and tissue of fetuses within two examined cases of CSF in non-immune pregnant sows. In the third examined case of CSF, the material comprised the blood of piglets before suckling the colostrum, animals originating from vaccinated sows, at a farm in which CSF had been diagnosed. Samples of tissue and blood of the piglets were examined for the presence of antigens and specific antibodies against the CSF virus using the immunoenzyme technique (ELISA). Even though the investigations were performed on a small number of samples, the obtained results raise the question of the possibility of the occurrence of intrauterine infection with a CSF field virus in sows vaccinated with the C-strain of CSF. The syndrome of a carrier sow and persistent infections are the chief problem factors that need to be considered within the programme of curbing and eradicating classical swine plague.
AB  - Ukoliko se suprasna nevakcinisana krmača inficira virusom klasične kuge svinja (KKS) dolazi do nastanka intrauterine infekcije fetusa. Posledica infekcije fetusa je pojava viremije. Distribucija virusa u njihovim tkivima je slična onoj koja se ustanovljava kod postnatalno inficiranih svinja. Cilj ispitivanja je bio ustanovljavanje intrauterine (transplacentarne) infekcije u slučaju pojave KKS u različitim periodima suprasnosti kod nevakcinisanih i vakcinisanih krmača. U okviru dva slučaja KKS kod neimunih suprasnih krmača materijal za ispitivanja je obuhvatao organe i tkiva fetusa. U trećem ispitivanom slučaju KKS, materijal je obuhvatao krv prasadi pre sisanja kolostruma, koja su bila poreklom od vakcinisanih krmača, na farmi gde je dijagnostikovana KKS. Uzorci tkiva i krvi prasadi su ispitivani na prisustvo antigena i specifičnih antitela protiv virusa KKS imunoenzimskom (ELISA) tehnikom. Iako su ispitivanja obavljena na malom broju uzoraka, dobijeni rezultati nameću pitanje mogućnosti nastanka intrauterine infekcije terenskim virusom KKS kod krmača vakcinisanih K-sojem virusa KKS. Sindrom krmače kliconoše i perzistentne infekcije su glavni otežavajući faktori koje je potrebno dodatno sagledavati u okviru programa suzbijanja i eradikacije KKS.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Significance of determining intrauterine infections with classical swine plague virus within programme of curbing and eradicating this disease
T1  - Značaj ustanovljavanja intrauterinih infekcija virusom klasične kuge svinja u sklopu programa suzbijanja i iskorenjivanja oboljenja
VL  - 61
IS  - 3-4
SP  - 163
EP  - 171
DO  - 10.2298/VETGL0704163P
ER  - 

@article{
author = "Prodanov, Jasna and Došen, Radoslav and Petrović, Tamaš and Lupulović, Diana and Valčić, Miroslav and Polaček, Vladimir",
year = "2007",
abstract = "Intrauterine infection of the fetus occurs if a pregnant non-vaccinated sow is infected with the virus of classical swine plague (CSF). The infection of the fetus results in the occurrence of viremia and the distribution of the virus in fetal tissue is similar to the distribution which is established in post-natal infected swine. The objective of these investigations was to determine intrauterine (transplacental) infection in the event of the appearance of CSF in different periods of pregnancy in non-vaccinated and vaccinated sows. The examined material were organs and tissue of fetuses within two examined cases of CSF in non-immune pregnant sows. In the third examined case of CSF, the material comprised the blood of piglets before suckling the colostrum, animals originating from vaccinated sows, at a farm in which CSF had been diagnosed. Samples of tissue and blood of the piglets were examined for the presence of antigens and specific antibodies against the CSF virus using the immunoenzyme technique (ELISA). Even though the investigations were performed on a small number of samples, the obtained results raise the question of the possibility of the occurrence of intrauterine infection with a CSF field virus in sows vaccinated with the C-strain of CSF. The syndrome of a carrier sow and persistent infections are the chief problem factors that need to be considered within the programme of curbing and eradicating classical swine plague., Ukoliko se suprasna nevakcinisana krmača inficira virusom klasične kuge svinja (KKS) dolazi do nastanka intrauterine infekcije fetusa. Posledica infekcije fetusa je pojava viremije. Distribucija virusa u njihovim tkivima je slična onoj koja se ustanovljava kod postnatalno inficiranih svinja. Cilj ispitivanja je bio ustanovljavanje intrauterine (transplacentarne) infekcije u slučaju pojave KKS u različitim periodima suprasnosti kod nevakcinisanih i vakcinisanih krmača. U okviru dva slučaja KKS kod neimunih suprasnih krmača materijal za ispitivanja je obuhvatao organe i tkiva fetusa. U trećem ispitivanom slučaju KKS, materijal je obuhvatao krv prasadi pre sisanja kolostruma, koja su bila poreklom od vakcinisanih krmača, na farmi gde je dijagnostikovana KKS. Uzorci tkiva i krvi prasadi su ispitivani na prisustvo antigena i specifičnih antitela protiv virusa KKS imunoenzimskom (ELISA) tehnikom. Iako su ispitivanja obavljena na malom broju uzoraka, dobijeni rezultati nameću pitanje mogućnosti nastanka intrauterine infekcije terenskim virusom KKS kod krmača vakcinisanih K-sojem virusa KKS. Sindrom krmače kliconoše i perzistentne infekcije su glavni otežavajući faktori koje je potrebno dodatno sagledavati u okviru programa suzbijanja i eradikacije KKS.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Significance of determining intrauterine infections with classical swine plague virus within programme of curbing and eradicating this disease, Značaj ustanovljavanja intrauterinih infekcija virusom klasične kuge svinja u sklopu programa suzbijanja i iskorenjivanja oboljenja",
volume = "61",
number = "3-4",
pages = "163-171",
doi = "10.2298/VETGL0704163P"
}

Prodanov, J., Došen, R., Petrović, T., Lupulović, D., Valčić, M.,& Polaček, V.. (2007). Significance of determining intrauterine infections with classical swine plague virus within programme of curbing and eradicating this disease. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(3-4), 163-171.
https://doi.org/10.2298/VETGL0704163P

Prodanov J, Došen R, Petrović T, Lupulović D, Valčić M, Polaček V. Significance of determining intrauterine infections with classical swine plague virus within programme of curbing and eradicating this disease. in Veterinarski Glasnik. 2007;61(3-4):163-171.
doi:10.2298/VETGL0704163P .

Prodanov, Jasna, Došen, Radoslav, Petrović, Tamaš, Lupulović, Diana, Valčić, Miroslav, Polaček, Vladimir, "Significance of determining intrauterine infections with classical swine plague virus within programme of curbing and eradicating this disease" in Veterinarski Glasnik, 61, no. 3-4 (2007):163-171,
https://doi.org/10.2298/VETGL0704163P . .